Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001465.01.T01 | RHN78268.1 | 95.833 | 96 | 4 | 0 | 1 | 96 | 1 | 96 | 3.50E-57 | 188 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001465.01.T01 | A0A396JPS6 | 95.833 | 96 | 4 | 0 | 1 | 96 | 1 | 96 | 1.67e-57 | 188 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000741.01 | MsG0180001465.01 | 0.801943 | 7.094206e-49 | 3.431400e-46 |
| MsG0180001465.01 | MsG0180001662.01 | 0.806970 | 6.351851e-50 | 3.495076e-47 |
| MsG0180001465.01 | MsG0180003807.01 | 0.820248 | 7.582706e-53 | 5.968311e-50 |
| MsG0180001465.01 | MsG0280010109.01 | 0.809449 | 1.882349e-50 | 1.105478e-47 |
| MsG0180001465.01 | MsG0280010599.01 | 0.812850 | 3.445447e-51 | 2.214401e-48 |
| MsG0180001465.01 | MsG0280011201.01 | 0.816959 | 4.224785e-52 | 3.036968e-49 |
| MsG0180001465.01 | MsG0380012034.01 | 0.807299 | 5.409022e-50 | 3.001944e-47 |
| MsG0180001465.01 | MsG0380013686.01 | 0.806265 | 8.946151e-50 | 4.834569e-47 |
| MsG0180001465.01 | MsG0380015980.01 | 0.806688 | 7.286581e-50 | 3.980165e-47 |
| MsG0180001465.01 | MsG0480019453.01 | 0.808980 | 2.372196e-50 | 1.375808e-47 |
| MsG0180001465.01 | MsG0480020505.01 | 0.803193 | 3.917068e-49 | 1.956130e-46 |
| MsG0180001465.01 | MsG0480022203.01 | 0.822721 | 2.035759e-53 | 1.717762e-50 |
| MsG0180001465.01 | MsG0680034235.01 | 0.809093 | 2.243221e-50 | 1.305010e-47 |
| MsG0180001465.01 | MsG0780039717.01 | 0.812389 | 4.345725e-51 | 2.759565e-48 |
| MsG0180001465.01 | MsG0880043480.01 | 0.803428 | 3.502271e-49 | 1.759533e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001465.01.T01 | MTR_1g036520 | 95.833 | 96 | 4 | 0 | 1 | 96 | 1 | 96 | 2.43e-57 | 190 |
| MsG0180001465.01.T01 | MTR_0006s0230 | 94.792 | 96 | 5 | 0 | 1 | 96 | 1 | 96 | 2.61e-56 | 187 |
| MsG0180001465.01.T01 | MTR_1g037010 | 94.792 | 96 | 5 | 0 | 1 | 96 | 1 | 96 | 2.81e-56 | 187 |
| MsG0180001465.01.T01 | MTR_1g037250 | 95.833 | 96 | 4 | 0 | 1 | 96 | 1 | 96 | 3.32e-56 | 187 |
| MsG0180001465.01.T01 | MTR_1g051670 | 81.818 | 99 | 18 | 0 | 1 | 99 | 1 | 99 | 3.63e-54 | 170 |
| MsG0180001465.01.T01 | MTR_0006s0080 | 88.542 | 96 | 11 | 0 | 1 | 96 | 1 | 96 | 1.43e-52 | 177 |
| MsG0180001465.01.T01 | MTR_0069s0040 | 83.838 | 99 | 16 | 0 | 1 | 99 | 1 | 99 | 7.78e-51 | 172 |
| MsG0180001465.01.T01 | MTR_1g051585 | 76.147 | 109 | 26 | 0 | 1 | 109 | 1 | 109 | 4.36e-50 | 170 |
| MsG0180001465.01.T01 | MTR_8g059345 | 85.417 | 96 | 14 | 0 | 1 | 96 | 1 | 96 | 7.08e-50 | 169 |
| MsG0180001465.01.T01 | MTR_1g037130 | 83.333 | 96 | 16 | 0 | 1 | 96 | 1 | 96 | 2.50e-49 | 167 |
| MsG0180001465.01.T01 | MTR_0006s0140 | 80.208 | 96 | 19 | 0 | 1 | 96 | 1 | 96 | 5.37e-48 | 157 |
| MsG0180001465.01.T01 | MTR_6g463150 | 84.375 | 96 | 15 | 0 | 1 | 96 | 1 | 96 | 5.60e-48 | 164 |
| MsG0180001465.01.T01 | MTR_1g037150 | 79.167 | 96 | 20 | 0 | 1 | 96 | 1 | 96 | 6.56e-48 | 164 |
| MsG0180001465.01.T01 | MTR_0006s0240 | 79.167 | 96 | 20 | 0 | 1 | 96 | 1 | 96 | 4.41e-47 | 161 |
| MsG0180001465.01.T01 | MTR_1g442750 | 80.808 | 99 | 19 | 0 | 1 | 99 | 1 | 99 | 5.56e-47 | 161 |
| MsG0180001465.01.T01 | MTR_0068s0080 | 81.250 | 96 | 18 | 0 | 1 | 96 | 1 | 96 | 5.71e-47 | 161 |
| MsG0180001465.01.T01 | MTR_0068s0080 | 81.250 | 96 | 18 | 0 | 1 | 96 | 1 | 96 | 8.53e-47 | 160 |
| MsG0180001465.01.T01 | MTR_1g036800 | 80.208 | 96 | 19 | 0 | 1 | 96 | 1 | 96 | 1.24e-45 | 157 |
| MsG0180001465.01.T01 | MTR_1g041550 | 79.167 | 96 | 20 | 0 | 1 | 96 | 1 | 96 | 1.58e-45 | 157 |
| MsG0180001465.01.T01 | MTR_1480s0010 | 89.474 | 76 | 8 | 0 | 1 | 76 | 1 | 76 | 6.17e-45 | 140 |
| MsG0180001465.01.T01 | MTR_2g089975 | 79.167 | 96 | 20 | 0 | 1 | 96 | 1 | 96 | 7.27e-45 | 155 |
| MsG0180001465.01.T01 | MTR_0406s0030 | 77.083 | 96 | 22 | 0 | 1 | 96 | 1 | 96 | 1.89e-44 | 154 |
| MsG0180001465.01.T01 | MTR_1g044020 | 70.833 | 96 | 28 | 0 | 1 | 96 | 1 | 96 | 1.14e-40 | 143 |
| MsG0180001465.01.T01 | MTR_1g036860 | 69.792 | 96 | 29 | 0 | 1 | 96 | 1 | 96 | 1.23e-37 | 132 |
| MsG0180001465.01.T01 | MTR_1g036750 | 63.542 | 96 | 3 | 1 | 1 | 96 | 1 | 64 | 4.08e-28 | 107 |
| MsG0180001465.01.T01 | MTR_1g050402 | 45.946 | 111 | 55 | 1 | 1 | 111 | 1 | 106 | 9.24e-27 | 103 |
| MsG0180001465.01.T01 | MTR_2g072020 | 45.833 | 96 | 52 | 0 | 1 | 96 | 1 | 96 | 3.04e-24 | 96.3 |
| MsG0180001465.01.T01 | MTR_2g072820 | 42.708 | 96 | 55 | 0 | 1 | 96 | 1 | 96 | 1.65e-23 | 94.4 |
| MsG0180001465.01.T01 | MTR_2g072080 | 43.000 | 100 | 57 | 0 | 1 | 100 | 1 | 100 | 2.24e-23 | 94.0 |
| MsG0180001465.01.T01 | MTR_2g072150 | 42.708 | 96 | 55 | 0 | 1 | 96 | 1 | 96 | 2.10e-21 | 88.2 |
| MsG0180001465.01.T01 | MTR_2g072030 | 41.667 | 96 | 56 | 0 | 1 | 96 | 1 | 96 | 3.61e-21 | 87.4 |
| MsG0180001465.01.T01 | MTR_2g071820 | 47.059 | 85 | 45 | 0 | 1 | 85 | 1 | 85 | 8.49e-20 | 83.6 |
| MsG0180001465.01.T01 | MTR_2g071890 | 44.706 | 85 | 47 | 0 | 1 | 85 | 1 | 85 | 1.02e-19 | 83.6 |
| MsG0180001465.01.T01 | MTR_2g072110 | 36.458 | 96 | 61 | 0 | 1 | 96 | 1 | 96 | 1.86e-19 | 82.8 |
| MsG0180001465.01.T01 | MTR_2g072110 | 36.458 | 96 | 61 | 0 | 1 | 96 | 1 | 96 | 6.77e-19 | 80.9 |
| MsG0180001465.01.T01 | MTR_2g072090 | 38.542 | 96 | 59 | 0 | 1 | 96 | 5 | 100 | 9.86e-19 | 80.5 |
| MsG0180001465.01.T01 | MTR_2g071870 | 38.542 | 96 | 59 | 0 | 1 | 96 | 5 | 100 | 9.99e-19 | 80.5 |
| MsG0180001465.01.T01 | MTR_2g071960 | 37.500 | 96 | 60 | 0 | 1 | 96 | 5 | 100 | 3.35e-18 | 79.0 |
| MsG0180001465.01.T01 | MTR_2g071850 | 37.500 | 96 | 60 | 0 | 1 | 96 | 5 | 100 | 4.41e-18 | 78.6 |
| MsG0180001465.01.T01 | MTR_4g025470 | 49.275 | 69 | 35 | 0 | 28 | 96 | 4 | 72 | 5.02e-18 | 78.6 |
| MsG0180001465.01.T01 | MTR_4g025430 | 49.275 | 69 | 35 | 0 | 28 | 96 | 4 | 72 | 8.28e-18 | 77.8 |
| MsG0180001465.01.T01 | MTR_2g071940 | 38.542 | 96 | 59 | 0 | 1 | 96 | 5 | 100 | 1.14e-17 | 77.4 |
| MsG0180001465.01.T01 | MTR_2g071900 | 39.583 | 96 | 54 | 1 | 1 | 96 | 5 | 96 | 2.93e-16 | 73.6 |
| MsG0180001465.01.T01 | MTR_2g071980 | 36.458 | 96 | 57 | 1 | 1 | 96 | 5 | 96 | 2.32e-14 | 68.2 |
| MsG0180001465.01.T01 | MTR_2g075970 | 36.264 | 91 | 58 | 0 | 6 | 96 | 3 | 93 | 3.50e-13 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTAAAAGTGAAAGGGAAAA+TGG | 0.313407 | 1:+21761483 | MsG0180001465.01.T01:CDS |
| GAATATACCGTTGTGCCTAT+TGG | 0.372128 | 1:+21761360 | MsG0180001465.01.T01:CDS |
| AGGTAACTTCAAGACATTAA+AGG | 0.373324 | 1:+21761413 | MsG0180001465.01.T01:CDS |
| GTTAGGTGTGATGAAATTTA+CGG | 0.416577 | 1:+21761603 | MsG0180001465.01.T01:CDS |
| TAACTTGCTTGACGTCCAAT+AGG | 0.441814 | 1:-21761375 | None:intergenic |
| TTCTTTCTCTTGCAGCCTCA+AGG | 0.459948 | 1:-21761449 | None:intergenic |
| ATTCTCGCAATGCAAATGTT+AGG | 0.467921 | 1:+21761586 | MsG0180001465.01.T01:CDS |
| TGCATTCAGTTAAAAGTGAA+AGG | 0.490594 | 1:+21761475 | MsG0180001465.01.T01:CDS |
| AAAAGATGTTCTGAATTGGT+TGG | 0.520491 | 1:+21761518 | MsG0180001465.01.T01:CDS |
| TCTGAATTGGTTGGAGAAAG+TGG | 0.527689 | 1:+21761527 | MsG0180001465.01.T01:CDS |
| GCATTCAGTTAAAAGTGAAA+GGG | 0.561889 | 1:+21761476 | MsG0180001465.01.T01:CDS |
| ATTGGTTGGAGAAAGTGGAC+GGG | 0.577110 | 1:+21761532 | MsG0180001465.01.T01:CDS |
| AGTGGACGGGGTCATTAAAG+AGG | 0.589878 | 1:+21761545 | MsG0180001465.01.T01:CDS |
| AATTGGTTGGAGAAAGTGGA+CGG | 0.609642 | 1:+21761531 | MsG0180001465.01.T01:CDS |
| TTGGTTGGAGAAAGTGGACG+GGG | 0.646949 | 1:+21761533 | MsG0180001465.01.T01:CDS |
| GGATCATGTTGAAGACCTTG+AGG | 0.651582 | 1:+21761434 | MsG0180001465.01.T01:CDS |
| AGTTACTTGATATTCTACAA+AGG | 0.666592 | 1:+21761393 | MsG0180001465.01.T01:CDS |
| ACAGTTATAGACACAAAACA+TGG | 0.711501 | 1:+21761634 | MsG0180001465.01.T01:CDS |
| TTGACGTCCAATAGGCACAA+CGG | 0.724308 | 1:-21761367 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAAATTTTAATTTCTATTGT+TGG | + | Chr1:21761327-21761346 | MsG0180001465.01.T01:CDS | 15.0% |
| ! | AGTTACTTGATATTCTACAA+AGG | + | Chr1:21761393-21761412 | MsG0180001465.01.T01:CDS | 25.0% |
| !! | TTGAAAAAGATGTTCTGAAT+TGG | + | Chr1:21761514-21761533 | MsG0180001465.01.T01:CDS | 25.0% |
| ACAGTTATAGACACAAAACA+TGG | + | Chr1:21761634-21761653 | MsG0180001465.01.T01:CDS | 30.0% | |
| AGGTAACTTCAAGACATTAA+AGG | + | Chr1:21761413-21761432 | MsG0180001465.01.T01:CDS | 30.0% | |
| GCATTCAGTTAAAAGTGAAA+GGG | + | Chr1:21761476-21761495 | MsG0180001465.01.T01:CDS | 30.0% | |
| GTTAAAAGTGAAAGGGAAAA+TGG | + | Chr1:21761483-21761502 | MsG0180001465.01.T01:CDS | 30.0% | |
| GTTAGGTGTGATGAAATTTA+CGG | + | Chr1:21761603-21761622 | MsG0180001465.01.T01:CDS | 30.0% | |
| TGCATTCAGTTAAAAGTGAA+AGG | + | Chr1:21761475-21761494 | MsG0180001465.01.T01:CDS | 30.0% | |
| ! | AAAAGATGTTCTGAATTGGT+TGG | + | Chr1:21761518-21761537 | MsG0180001465.01.T01:CDS | 30.0% |
| ATTCTCGCAATGCAAATGTT+AGG | + | Chr1:21761586-21761605 | MsG0180001465.01.T01:CDS | 35.0% | |
| AATTGGTTGGAGAAAGTGGA+CGG | + | Chr1:21761531-21761550 | MsG0180001465.01.T01:CDS | 40.0% | |
| GAATATACCGTTGTGCCTAT+TGG | + | Chr1:21761360-21761379 | MsG0180001465.01.T01:CDS | 40.0% | |
| TCTGAATTGGTTGGAGAAAG+TGG | + | Chr1:21761527-21761546 | MsG0180001465.01.T01:CDS | 40.0% | |
| !! | TAACTTGCTTGACGTCCAAT+AGG | - | Chr1:21761378-21761397 | None:intergenic | 40.0% |
| ATTGGTTGGAGAAAGTGGAC+GGG | + | Chr1:21761532-21761551 | MsG0180001465.01.T01:CDS | 45.0% | |
| GGATCATGTTGAAGACCTTG+AGG | + | Chr1:21761434-21761453 | MsG0180001465.01.T01:CDS | 45.0% | |
| TTCTTTCTCTTGCAGCCTCA+AGG | - | Chr1:21761452-21761471 | None:intergenic | 45.0% | |
| TTGACGTCCAATAGGCACAA+CGG | - | Chr1:21761370-21761389 | None:intergenic | 45.0% | |
| AGTGGACGGGGTCATTAAAG+AGG | + | Chr1:21761545-21761564 | MsG0180001465.01.T01:CDS | 50.0% | |
| TTGGTTGGAGAAAGTGGACG+GGG | + | Chr1:21761533-21761552 | MsG0180001465.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 21761324 | 21761668 | 21761324 | ID=MsG0180001465.01;Name=MsG0180001465.01 |
| Chr1 | mRNA | 21761324 | 21761668 | 21761324 | ID=MsG0180001465.01.T01;Parent=MsG0180001465.01;Name=MsG0180001465.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|114 |
| Chr1 | exon | 21761324 | 21761668 | 21761324 | ID=MsG0180001465.01.T01:exon:13665;Parent=MsG0180001465.01.T01 |
| Chr1 | CDS | 21761324 | 21761668 | 21761324 | ID=MsG0180001465.01.T01:cds;Parent=MsG0180001465.01.T01 |
| Gene Sequence |
| Protein sequence |
