AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380015980.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 14 sgRNAs with CRISPR-Local

Find 15 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGATTGCATGGTGAAATT+AGG 0.142450 3:+77437061 None:intergenic
GCTTCGGAAGCAGCTGAATA+AGG 0.294418 3:+77436983 None:intergenic
AAAGAACGACGCGATTGAAT+TGG 0.324086 3:+77437091 None:intergenic
AAGATTGCATGGTGAAATTA+GGG 0.336979 3:+77437062 None:intergenic
CGATTGAATTGGATTGGTTA+AGG 0.371271 3:+77437102 None:intergenic
GATCGAATGCCGAACGAAAT+CGG 0.380339 3:+77437005 None:intergenic
ATTCCGTCTCCGATTTCGTT+CGG 0.450786 3:-77437014 MsG0380015980.01.T01:CDS
ACGACGCGATTGAATTGGAT+TGG 0.484793 3:+77437096 None:intergenic
ACATCCGACAGAAGACATAC+TGG 0.490917 3:+77437039 None:intergenic
ATGCCGAACGAAATCGGAGA+CGG 0.560269 3:+77437011 None:intergenic
AGACATACTGGAAGATTGCA+TGG 0.601467 3:+77437051 None:intergenic
TCTTCCAGTATGTCTTCTGT+CGG 0.632142 3:-77437043 MsG0380015980.01.T01:CDS
GAGTGAGAATACGAAGAGCG+AGG 0.672758 3:+77436945 None:intergenic
TGAGAATACGAAGAGCGAGG+TGG 0.703974 3:+77436948 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAGATTGCATGGTGAAATTA+GGG + Chr3:77437008-77437027 None:intergenic 30.0%
GAAGATTGCATGGTGAAATT+AGG + Chr3:77437009-77437028 None:intergenic 35.0%
! GTGGTTTTCAGAAAAAGCTT+CGG + Chr3:77437103-77437122 None:intergenic 35.0%
!! CGATTGAATTGGATTGGTTA+AGG + Chr3:77436968-77436987 None:intergenic 35.0%
AAAGAACGACGCGATTGAAT+TGG + Chr3:77436979-77436998 None:intergenic 40.0%
AGACATACTGGAAGATTGCA+TGG + Chr3:77437019-77437038 None:intergenic 40.0%
TCTTCCAGTATGTCTTCTGT+CGG - Chr3:77437024-77437043 MsG0380015980.01.T01:CDS 40.0%
ACATCCGACAGAAGACATAC+TGG + Chr3:77437031-77437050 None:intergenic 45.0%
ACGACGCGATTGAATTGGAT+TGG + Chr3:77436974-77436993 None:intergenic 45.0%
ATTCCGTCTCCGATTTCGTT+CGG - Chr3:77437053-77437072 MsG0380015980.01.T01:CDS 45.0%
GATCGAATGCCGAACGAAAT+CGG + Chr3:77437065-77437084 None:intergenic 45.0%
ATGCCGAACGAAATCGGAGA+CGG + Chr3:77437059-77437078 None:intergenic 50.0%
GAGTGAGAATACGAAGAGCG+AGG + Chr3:77437125-77437144 None:intergenic 50.0%
GCTTCGGAAGCAGCTGAATA+AGG + Chr3:77437087-77437106 None:intergenic 50.0%
TGAGAATACGAAGAGCGAGG+TGG + Chr3:77437122-77437141 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr3 gene 77436940 77437149 77436940 ID=MsG0380015980.01;Name=MsG0380015980.01
Chr3 mRNA 77436940 77437149 77436940 ID=MsG0380015980.01.T01;Parent=MsG0380015980.01;Name=MsG0380015980.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|69
Chr3 exon 77436940 77437149 77436940 ID=MsG0380015980.01.T01:exon:25099;Parent=MsG0380015980.01.T01
Chr3 CDS 77436940 77437149 77436940 ID=MsG0380015980.01.T01:cds;Parent=MsG0380015980.01.T01
Gene Sequence

>MsG0380015980.01.T01

ATGGAATTACGTTCGATTCAATTCTCCTTAACCAATCCAATTCAATCGCGTCGTTCTTTTCAAAACCCTAATTTCACCATGCAATCTTCCAGTATGTCTTCTGTCGGATGTCGATTCCGTCTCCGATTTCGTTCGGCATTCGATCCTTATTCAGCTGCTTCCGAAGCTTTTTCTGAAAACCACCTCGCTCTTCGTATTCTCACTCTATAG

Protein sequence

>MsG0380015980.01.T01

MELRSIQFSLTNPIQSRRSFQNPNFTMQSSSMSSVGCRFRLRFRSAFDPYSAASEAFSENHLALRILTL*