AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000401.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000401.01.T01 MTR_1g016750 81.410 156 20 3 13 159 79 234 1.57e-74 224
MsG0180000401.01.T01 MTR_1g016750 100.000 35 0 0 1 35 1 35 1.53e-16 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000401.01.T01 AT5G42040 64.706 187 31 1 1 152 1 187 8.39e-78 231
MsG0180000401.01.T01 AT1G64520 85.577 104 15 0 49 152 118 221 4.85e-61 189

Find 39 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAGAAACTTCTGTGAATT+TGG 0.038934 1:+5540127 None:intergenic
TGGGATCCATATCGTGATTT+TGG 0.114449 1:+5540147 None:intergenic
GGAGTTGGAGCAATCCTTTA+TGG 0.271340 1:-5537108 MsG0180000401.01.T01:CDS
TGTGTATTTCATGGATCTTT+TGG 0.284808 1:-5537030 MsG0180000401.01.T01:CDS
TTCCAAGAGTTCCTGGTCTT+TGG 0.286082 1:+5536582 None:intergenic
AAATCTCCTTTCCCAGCTTA+AGG 0.331927 1:-5540051 MsG0180000401.01.T01:intron
GGAACTCTTGGAATATATTA+AGG 0.340067 1:-5536572 MsG0180000401.01.T01:CDS
TGCTGAATTCCATACTGAAT+TGG 0.350776 1:-5537180 MsG0180000401.01.T01:CDS
TGAGAAACTTCTGTGAATTT+GGG 0.351752 1:+5540128 None:intergenic
TAATATATTCCAAGAGTTCC+TGG 0.370117 1:+5536575 None:intergenic
TGAGACTTCTTGTGCAGAAT+AGG 0.396052 1:-5537205 MsG0180000401.01.T01:CDS
TGAAACATATGTGTATTTCA+TGG 0.407221 1:-5537039 MsG0180000401.01.T01:CDS
AAAGCAATTCCAATTCAGTA+TGG 0.408550 1:+5537171 None:intergenic
CAATTCCCAAAATCACGATA+TGG 0.413083 1:-5540153 MsG0180000401.01.T01:exon
CACCAAAGACCAGGAACTCT+TGG 0.427127 1:-5536584 MsG0180000401.01.T01:CDS
ATTGCTTTCATCCACTGCTC+TGG 0.436359 1:-5537156 MsG0180000401.01.T01:CDS
GTTGTATGCCCCTTCCATAA+AGG 0.451259 1:+5537094 None:intergenic
CCTCAGGAGTACCCAATACT+TGG 0.457349 1:-5537239 MsG0180000401.01.T01:intron
ACAGGTTGAGACCAAGTATT+GGG 0.478577 1:+5537228 None:intergenic
GGTTAGAGTGTACCTTAAGC+TGG 0.480510 1:+5540039 None:intergenic
TCTGCACAAGAAGTCTCAAC+AGG 0.487747 1:+5537210 None:intergenic
TATTAAGCATGCCGTGGAGT+TGG 0.497949 1:-5537123 MsG0180000401.01.T01:CDS
GGAGCAATCCTTTATGGAAG+GGG 0.535947 1:-5537102 MsG0180000401.01.T01:CDS
TTGGAGCAATCCTTTATGGA+AGG 0.551519 1:-5537104 MsG0180000401.01.T01:CDS
AACAGGTTGAGACCAAGTAT+TGG 0.562540 1:+5537227 None:intergenic
GTTAGAGTGTACCTTAAGCT+GGG 0.574520 1:+5540040 None:intergenic
TGGAGCAATCCTTTATGGAA+GGG 0.575444 1:-5537103 MsG0180000401.01.T01:CDS
ACGTTCAGAGATGAGATAGC+AGG 0.577525 1:-5536667 MsG0180000401.01.T01:intron
GATAGCAGGATGCAGTGAGA+AGG 0.587670 1:-5536653 MsG0180000401.01.T01:CDS
TCCACGGCATGCTTAATACA+AGG 0.594862 1:+5537128 None:intergenic
AGTGTACCTTAAGCTGGGAA+AGG 0.604789 1:+5540045 None:intergenic
ACAAGGATTCTCCAGAGCAG+TGG 0.619513 1:+5537145 None:intergenic
AAAGGATTGCTCCAACTCCA+CGG 0.630188 1:+5537112 None:intergenic
TCTGTCGAGCACTCAAGACA+CGG 0.643446 1:+5537072 None:intergenic
TCCTTGTATTAAGCATGCCG+TGG 0.644930 1:-5537129 MsG0180000401.01.T01:CDS
GTTGCTGTTCACCAAAGACC+AGG 0.651601 1:-5536593 MsG0180000401.01.T01:CDS
AAATACACATATGTTTCATG+TGG 0.652663 1:+5537044 None:intergenic
ACTCTTGGAATATATTAAGG+AGG 0.657443 1:-5536569 MsG0180000401.01.T01:CDS
CTGGGAAAGGAGATTTGAAG+CGG 0.672348 1:+5540058 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTAAAAAAAATAAATAGA+TGG + Chr1:5539837-5539856 None:intergenic 10.0%
!! ATTTGTATAAAAAAATTAAG+AGG - Chr1:5536874-5536893 MsG0180000401.01.T01:intron 10.0%
!! TATATGTATATTACTAAAAT+AGG + Chr1:5538550-5538569 None:intergenic 10.0%
!! TTTGTATAAAAAAATTAAGA+GGG - Chr1:5536875-5536894 MsG0180000401.01.T01:intron 10.0%
!!! AAATTAATTATGTCAATTTT+GGG - Chr1:5536812-5536831 MsG0180000401.01.T01:intron 10.0%
!!! TAAATTAATTATGTCAATTT+TGG - Chr1:5536811-5536830 MsG0180000401.01.T01:intron 10.0%
!!! TTTATTTTGTATAATTGATA+TGG + Chr1:5538992-5539011 None:intergenic 10.0%
!! AATCTTAAATTAAATTAGGT+TGG - Chr1:5538763-5538782 MsG0180000401.01.T01:intron 15.0%
!! AATTATTTCATAATCAATTC+TGG - Chr1:5538695-5538714 MsG0180000401.01.T01:intron 15.0%
!! AGTGAATCTTAAATTAAATT+AGG - Chr1:5538759-5538778 MsG0180000401.01.T01:intron 15.0%
!! ATTACTAATAACACAAAATA+CGG + Chr1:5538482-5538501 None:intergenic 15.0%
!! TTGTATAAAAAAATTAAGAG+GGG - Chr1:5536876-5536895 MsG0180000401.01.T01:intron 15.0%
!!! CACTAAAATCTTTTATTTTT+CGG + Chr1:5537339-5537358 None:intergenic 15.0%
!!! CTAAAATATGTTTCTTTTAT+AGG + Chr1:5537103-5537122 None:intergenic 15.0%
!!! TAACATAATTGATTTATGTT+TGG + Chr1:5538639-5538658 None:intergenic 15.0%
!!! TATTGTAAATGACATTTTAA+TGG - Chr1:5538113-5538132 MsG0180000401.01.T01:intron 15.0%
!! AAATTCTCGACAAATAATTA+TGG + Chr1:5537722-5537741 None:intergenic 20.0%
!! ATATGAGTGAATCTTAAATT+AGG - Chr1:5538087-5538106 MsG0180000401.01.T01:intron 20.0%
!! GAACAAAAAAGTAAAAATAC+AGG + Chr1:5537999-5538018 None:intergenic 20.0%
!! TAATAAACTCAGTTATTGTA+GGG - Chr1:5537677-5537696 MsG0180000401.01.T01:intron 20.0%
!! TGTGTTAATTTATGATGTTT+GGG + Chr1:5538595-5538614 None:intergenic 20.0%
!! TTAATAAACTCAGTTATTGT+AGG - Chr1:5537676-5537695 MsG0180000401.01.T01:intron 20.0%
!! TTATGGAAAACTAAATGATA+AGG + Chr1:5537705-5537724 None:intergenic 20.0%
!! TTGTATAATTGATATGGAAA+AGG + Chr1:5538986-5539005 None:intergenic 20.0%
!! TTGTGTTAATTTATGATGTT+TGG + Chr1:5538596-5538615 None:intergenic 20.0%
!!! AAAAGAAACATATTTTAGAG+AGG - Chr1:5537105-5537124 MsG0180000401.01.T01:CDS 20.0%
!!! ACAAGAGTTTTTACTAAAAT+TGG - Chr1:5538918-5538937 MsG0180000401.01.T01:intron 20.0%
!!! AGATAGATAAATTTTCTATC+TGG - Chr1:5538826-5538845 MsG0180000401.01.T01:intron 20.0%
!!! ATTTTTTTTACTTCTGAAGT+TGG - Chr1:5539844-5539863 MsG0180000401.01.T01:intron 20.0%
!!! CGAAAAATAAAAGATTTTAG+TGG - Chr1:5537337-5537356 MsG0180000401.01.T01:intron 20.0%
! AAAAAAAACAAGCAACTGAA+TGG + Chr1:5537943-5537962 None:intergenic 25.0%
! AAATACACATATGTTTCATG+TGG + Chr1:5539716-5539735 None:intergenic 25.0%
! AATTAATATGATCGAGTCAT+GGG + Chr1:5539031-5539050 None:intergenic 25.0%
! ATAACCTAATGACCTAAATA+AGG - Chr1:5536989-5537008 MsG0180000401.01.T01:intron 25.0%
! CACTCATATGATATTTGATA+AGG + Chr1:5538076-5538095 None:intergenic 25.0%
! CTACAAGGTAATATACTATA+AGG + Chr1:5539364-5539383 None:intergenic 25.0%
! GATATTCTTGTGAAGTAAAA+TGG - Chr1:5537147-5537166 MsG0180000401.01.T01:CDS 25.0%
! TAAAAATAGTACCTAATTGC+TGG - Chr1:5537763-5537782 MsG0180000401.01.T01:intron 25.0%
! TAATTAATATGATCGAGTCA+TGG + Chr1:5539032-5539051 None:intergenic 25.0%
! TCATATGATATTTGATAAGG+TGG + Chr1:5538073-5538092 None:intergenic 25.0%
! TGAAACATATGTGTATTTCA+TGG - Chr1:5539718-5539737 MsG0180000401.01.T01:intron 25.0%
! TGTTGAGCTATTGTAATTTA+TGG - Chr1:5539457-5539476 MsG0180000401.01.T01:intron 25.0%
! TTGAAAGAAATCTCTTTCAA+AGG + Chr1:5539209-5539228 None:intergenic 25.0%
!! AGATAAATTTTCTATCTGGT+TGG - Chr1:5538830-5538849 MsG0180000401.01.T01:intron 25.0%
!! ATTGTGTACTTGTTAACAAA+GGG + Chr1:5539272-5539291 None:intergenic 25.0%
!! CTTCTTGTTGAAAATTTTCA+GGG - Chr1:5539132-5539151 MsG0180000401.01.T01:intron 25.0%
!! CTTGATACTTGTTAGTTATT+GGG - Chr1:5537444-5537463 MsG0180000401.01.T01:intron 25.0%
!! TATTGTGTACTTGTTAACAA+AGG + Chr1:5539273-5539292 None:intergenic 25.0%
!! TCTTCTTGTTGAAAATTTTC+AGG - Chr1:5539131-5539150 MsG0180000401.01.T01:intron 25.0%
!! TCTTGATACTTGTTAGTTAT+TGG - Chr1:5537443-5537462 MsG0180000401.01.T01:intron 25.0%
!! TGAATATAATTGCGCATTTT+GGG - Chr1:5537195-5537214 MsG0180000401.01.T01:CDS 25.0%
!! TTCTTGTTGAAAATTTTCAG+GGG - Chr1:5539133-5539152 MsG0180000401.01.T01:intron 25.0%
!!! AAATAAAAGATTTTAGTGGC+AGG - Chr1:5537341-5537360 MsG0180000401.01.T01:intron 25.0%
!!! AAATTAAATTAGGTTGGAAG+AGG - Chr1:5538769-5538788 MsG0180000401.01.T01:intron 25.0%
!!! TGACAAATACTGTATTTTAG+AGG - Chr1:5538885-5538904 MsG0180000401.01.T01:intron 25.0%
AAACCTAATACCCAATAGAA+AGG - Chr1:5537395-5537414 MsG0180000401.01.T01:intron 30.0%
AAACTAGTTAGCAATTGACA+TGG + Chr1:5540060-5540079 None:intergenic 30.0%
AAACTTTGTCTAACCGTTTA+AGG + Chr1:5537603-5537622 None:intergenic 30.0%
AAAGAAACTAGGGTTTAGTA+GGG + Chr1:5536742-5536761 None:intergenic 30.0%
AAAGCAATTCCAATTCAGTA+TGG + Chr1:5539589-5539608 None:intergenic 30.0%
ACATAAGTGTTTGTGAGAAA+CGG - Chr1:5537241-5537260 MsG0180000401.01.T01:intron 30.0%
ACTCTTGGAATATATTAAGG+AGG - Chr1:5540188-5540207 MsG0180000401.01.T01:five_prime_UTR 30.0%
AGCAATAGTTTCCTTTCTAT+TGG + Chr1:5537409-5537428 None:intergenic 30.0%
AGGTTAATAATGTTGTGCTT+CGG + Chr1:5539991-5540010 None:intergenic 30.0%
ATGGAAAAACTATACAACAG+AGG + Chr1:5540027-5540046 None:intergenic 30.0%
ATTGAACTCAACCGTTAAAT+TGG - Chr1:5539807-5539826 MsG0180000401.01.T01:intron 30.0%
CAAGAAAGCAAATAATGAGT+TGG + Chr1:5539439-5539458 None:intergenic 30.0%
CTAATGACCTAAATAAGGAA+CGG - Chr1:5536994-5537013 MsG0180000401.01.T01:intron 30.0%
GATAATACTTTCACGTTTCT+TGG + Chr1:5539393-5539412 None:intergenic 30.0%
GCAATAGTTTCCTTTCTATT+GGG + Chr1:5537408-5537427 None:intergenic 30.0%
GGAACTCTTGGAATATATTA+AGG - Chr1:5540185-5540204 MsG0180000401.01.T01:five_prime_UTR 30.0%
GTAGTTCCTGCAATAAAATT+TGG - Chr1:5539965-5539984 MsG0180000401.01.T01:intron 30.0%
GTTTCTTGGTATATACTACA+AGG + Chr1:5539379-5539398 None:intergenic 30.0%
TAAGATAATAACTGCTTGTC+AGG - Chr1:5539059-5539078 MsG0180000401.01.T01:intron 30.0%
TAATATATTCCAAGAGTTCC+TGG + Chr1:5540185-5540204 None:intergenic 30.0%
TAGATGGAGTTCCAATTTAA+CGG + Chr1:5539821-5539840 None:intergenic 30.0%
TATCTATCTTCTACTTACCA+TGG + Chr1:5538815-5538834 None:intergenic 30.0%
TGACAACAATTATTACCTGA+TGG + Chr1:5539759-5539778 None:intergenic 30.0%
TGAGAAACTTCTGTGAATTT+GGG + Chr1:5536632-5536651 None:intergenic 30.0%
TTGTCCTTAGTGTAAAATTG+AGG - Chr1:5539174-5539193 MsG0180000401.01.T01:intron 30.0%
TTGTGAAGTAAAATGGTTGT+GGG - Chr1:5537154-5537173 MsG0180000401.01.T01:CDS 30.0%
TTGTTAGTTATTGGGCTTAT+AGG - Chr1:5537452-5537471 MsG0180000401.01.T01:intron 30.0%
TTTCCTTTCTATTGGGTATT+AGG + Chr1:5537401-5537420 None:intergenic 30.0%
TTTCTCACAAACACTTATGT+GGG + Chr1:5537241-5537260 None:intergenic 30.0%
! CAATTTTACACTAAGGACAA+CGG + Chr1:5539174-5539193 None:intergenic 30.0%
! GTGAATATAATTGCGCATTT+TGG - Chr1:5537194-5537213 MsG0180000401.01.T01:CDS 30.0%
! TGATCCTCAATTTTACACTA+AGG + Chr1:5539181-5539200 None:intergenic 30.0%
! TGTGTATTTCATGGATCTTT+TGG - Chr1:5539727-5539746 MsG0180000401.01.T01:intron 30.0%
! TGTTAGAGAAATTAGTTTCG+AGG + Chr1:5539897-5539916 None:intergenic 30.0%
! TTCTCAATGTGACTTTTTAG+TGG - Chr1:5537807-5537826 MsG0180000401.01.T01:intron 30.0%
! TTGTGTACTTGTTAACAAAG+GGG + Chr1:5539271-5539290 None:intergenic 30.0%
!! AAGATGGTTTTTCAGAAACT+GGG - Chr1:5537025-5537044 MsG0180000401.01.T01:CDS 30.0%
!! CATTTTTCCGTTCCTTATTT+AGG + Chr1:5537004-5537023 None:intergenic 30.0%
!! TAAGATGGTTTTTCAGAAAC+TGG - Chr1:5537024-5537043 MsG0180000401.01.T01:CDS 30.0%
!!! CATGTCTATTGTTTTAGATC+CGG + Chr1:5539321-5539340 None:intergenic 30.0%
!!! GACATTTTAATGGATTATCC+TGG - Chr1:5538123-5538142 MsG0180000401.01.T01:intron 30.0%
!!! TTTTAAGACTATAGCCAGTT+TGG + Chr1:5537748-5537767 None:intergenic 30.0%
AAACTGGGTTTACTCTTGAT+TGG - Chr1:5537040-5537059 MsG0180000401.01.T01:CDS 35.0%
AAGGCAATAGTTAAGTGATC+AGG + Chr1:5539345-5539364 None:intergenic 35.0%
AGAAAGAATCACCAGCAATT+AGG + Chr1:5537777-5537796 None:intergenic 35.0%
AGGAACGGAAAAATGTAAGA+TGG - Chr1:5537009-5537028 MsG0180000401.01.T01:intron 35.0%
AGGAGAATGAACAAAGAGAA+AGG + Chr1:5538444-5538463 None:intergenic 35.0%
ATAAGTGTTTGTGAGAAACG+GGG - Chr1:5537243-5537262 MsG0180000401.01.T01:intron 35.0%
ATTAGGGTAGGAAAGAAACT+AGG + Chr1:5536753-5536772 None:intergenic 35.0%
ATTAGGTGTATCTGCAAACA+AGG + Chr1:5537880-5537899 None:intergenic 35.0%
CAATTCCCAAAATCACGATA+TGG - Chr1:5536604-5536623 MsG0180000401.01.T01:CDS 35.0%
CATAAGTGTTTGTGAGAAAC+GGG - Chr1:5537242-5537261 MsG0180000401.01.T01:intron 35.0%
CTAGAAGCTAAATCCTAACT+GGG + Chr1:5537521-5537540 None:intergenic 35.0%
CTGAGAAACTTCTGTGAATT+TGG + Chr1:5536633-5536652 None:intergenic 35.0%
CTTGTGAAGTAAAATGGTTG+TGG - Chr1:5537153-5537172 MsG0180000401.01.T01:CDS 35.0%
GAAAGAAACTAGGGTTTAGT+AGG + Chr1:5536743-5536762 None:intergenic 35.0%
GGAGAATGAACAAAGAGAAA+GGG + Chr1:5538443-5538462 None:intergenic 35.0%
GTTTCTCACAAACACTTATG+TGG + Chr1:5537242-5537261 None:intergenic 35.0%
TAATCTCAGACTGTTGAATC+CGG - Chr1:5539299-5539318 MsG0180000401.01.T01:intron 35.0%
TGATTCTTTGCCTATGAGAA+AGG - Chr1:5537577-5537596 MsG0180000401.01.T01:intron 35.0%
TGCTGAATTCCATACTGAAT+TGG - Chr1:5539577-5539596 MsG0180000401.01.T01:intron 35.0%
TTAAGAGGGGTTGTTAGAAA+CGG - Chr1:5536889-5536908 MsG0180000401.01.T01:intron 35.0%
TTAGGGTAGGAAAGAAACTA+GGG + Chr1:5536752-5536771 None:intergenic 35.0%
TTAGGTGTATCTGCAAACAA+GGG + Chr1:5537879-5537898 None:intergenic 35.0%
TTGACATGGATGTGCAAATA+TGG + Chr1:5540046-5540065 None:intergenic 35.0%
! ATAACCTTTCTGTCTGGTTT+AGG - Chr1:5538248-5538267 MsG0180000401.01.T01:intron 35.0%
! CAACACACAGCATTGAATTA+GGG + Chr1:5536769-5536788 None:intergenic 35.0%
! CGTTCCTTATTTAGGTCATT+AGG + Chr1:5536996-5537015 None:intergenic 35.0%
! TGGGTAGTTTGCATCAAAAT+TGG + Chr1:5538576-5538595 None:intergenic 35.0%
!! AGGATTTAGCTTCTAGCTTA+TGG - Chr1:5537525-5537544 MsG0180000401.01.T01:intron 35.0%
!! CATGCTTTGAAGAACAAAAC+AGG + Chr1:5538216-5538235 None:intergenic 35.0%
!! CTGTTTTGTTCTTCAAAGCA+TGG - Chr1:5538214-5538233 MsG0180000401.01.T01:intron 35.0%
!! GGATTTAGCTTCTAGCTTAT+GGG - Chr1:5537526-5537545 MsG0180000401.01.T01:intron 35.0%
!! TACGGATTAGAAAACAATGC+AGG + Chr1:5538464-5538483 None:intergenic 35.0%
!! TATCGTGATTTTGGGAATTG+CGG + Chr1:5536604-5536623 None:intergenic 35.0%
!! TTGTTTTGTTCCTCAAACCA+TGG - Chr1:5538795-5538814 MsG0180000401.01.T01:intron 35.0%
!! TTTACTTCTGAAGTTGGACT+TGG - Chr1:5539850-5539869 MsG0180000401.01.T01:intron 35.0%
AAATCTCCTTTCCCAGCTTA+AGG - Chr1:5536706-5536725 MsG0180000401.01.T01:intron 40.0%
AACCTTACTACACTGATGCA+CGG - Chr1:5539232-5539251 MsG0180000401.01.T01:intron 40.0%
AACGGTTGAGTTCAATCATC+AGG + Chr1:5539803-5539822 None:intergenic 40.0%
AACTGTTCTATCTGTTGTGC+TGG - Chr1:5538365-5538384 MsG0180000401.01.T01:intron 40.0%
ACGGTGTGTTTACTCTTGAT+TGG - Chr1:5536908-5536927 MsG0180000401.01.T01:intron 40.0%
AGTTCAATCATCAGGTAGGT+AGG + Chr1:5539795-5539814 None:intergenic 40.0%
CCTATGAGAAAGGCCTTAAA+CGG - Chr1:5537587-5537606 MsG0180000401.01.T01:intron 40.0%
CTTCTACTTACCATGGTTTG+AGG + Chr1:5538808-5538827 None:intergenic 40.0%
GCTAGAAGCTAAATCCTAAC+TGG + Chr1:5537522-5537541 None:intergenic 40.0%
GGATAGATAACCTTTCTGTC+TGG - Chr1:5538242-5538261 MsG0180000401.01.T01:intron 40.0%
GTTAGAGTGTACCTTAAGCT+GGG + Chr1:5536720-5536739 None:intergenic 40.0%
GTTGAGTTCAATCATCAGGT+AGG + Chr1:5539799-5539818 None:intergenic 40.0%
TAACTGCTTGTCAGGTGAAT+AGG - Chr1:5539067-5539086 MsG0180000401.01.T01:intron 40.0%
TAAGTGTTTGTGAGAAACGG+GGG - Chr1:5537244-5537263 MsG0180000401.01.T01:intron 40.0%
TACAGGTAAGAAAACAACGC+AGG + Chr1:5537982-5538001 None:intergenic 40.0%
TACGGGTAAGAAAACAATGC+AGG + Chr1:5538027-5538046 None:intergenic 40.0%
TGAGACTTCTTGTGCAGAAT+AGG - Chr1:5539552-5539571 MsG0180000401.01.T01:intron 40.0%
TGGAGCAATCCTTTATGGAA+GGG - Chr1:5539654-5539673 MsG0180000401.01.T01:intron 40.0%
TGGTTTATACTGACCCAGTT+AGG - Chr1:5537505-5537524 MsG0180000401.01.T01:intron 40.0%
TTCATTCCAATCAGTTGCTC+TGG - Chr1:5538413-5538432 MsG0180000401.01.T01:intron 40.0%
TTGGAGCAATCCTTTATGGA+AGG - Chr1:5539653-5539672 MsG0180000401.01.T01:intron 40.0%
TTTGTCATGCAGCAAGACAA+AGG + Chr1:5538872-5538891 None:intergenic 40.0%
! AACAGGTTGAGACCAAGTAT+TGG + Chr1:5539533-5539552 None:intergenic 40.0%
! ACACAGCATTGAATTAGGGT+AGG + Chr1:5536765-5536784 None:intergenic 40.0%
! ACAGGTTGAGACCAAGTATT+GGG + Chr1:5539532-5539551 None:intergenic 40.0%
! ATCTTTTGGCAAAGACCATC+AGG - Chr1:5539741-5539760 MsG0180000401.01.T01:intron 40.0%
! CTTCGGCCAAATTTTATTGC+AGG + Chr1:5539974-5539993 None:intergenic 40.0%
! GCAACACACAGCATTGAATT+AGG + Chr1:5536770-5536789 None:intergenic 40.0%
! GGGATCCATATCGTGATTTT+GGG + Chr1:5536612-5536631 None:intergenic 40.0%
! GTCGAGAATTTTGTCCAAAC+TGG - Chr1:5537731-5537750 MsG0180000401.01.T01:intron 40.0%
! GTTAGCTCTTGAACTGCATT+AGG + Chr1:5537897-5537916 None:intergenic 40.0%
! TAGTGGCATTTTTCACTTGC+AGG - Chr1:5537824-5537843 MsG0180000401.01.T01:intron 40.0%
! TGGGATCCATATCGTGATTT+TGG + Chr1:5536613-5536632 None:intergenic 40.0%
! TTGGGAATTGCGGTTTTCAA+AGG + Chr1:5536594-5536613 None:intergenic 40.0%
!! ACTTGCAGGTGTTACTAACA+GGG - Chr1:5537838-5537857 MsG0180000401.01.T01:intron 40.0%
!! AGATTTTAGTGGCAGGTTGT+TGG - Chr1:5537348-5537367 MsG0180000401.01.T01:intron 40.0%
!! CTCTTGATTGGTTGTTTCTG+TGG - Chr1:5537052-5537071 MsG0180000401.01.T01:CDS 40.0%
!! GAAGTGTGTGTTTAGTTCTG+CGG + Chr1:5538724-5538743 None:intergenic 40.0%
!! GATTTTAGTGGCAGGTTGTT+GGG - Chr1:5537349-5537368 MsG0180000401.01.T01:intron 40.0%
AAAGGATTGCTCCAACTCCA+CGG + Chr1:5539648-5539667 None:intergenic 45.0%
AACGGGGGTTTACTCTTGAT+TGG - Chr1:5537259-5537278 MsG0180000401.01.T01:intron 45.0%
ACGTTCAGAGATGAGATAGC+AGG - Chr1:5540090-5540109 MsG0180000401.01.T01:CDS 45.0%
AGGTTGTTGGGTGGCAATTT+GGG - Chr1:5537361-5537380 MsG0180000401.01.T01:intron 45.0%
AGTGTACCTTAAGCTGGGAA+AGG + Chr1:5536715-5536734 None:intergenic 45.0%
ATCACAAAGAGCTCATAGCC+AGG + Chr1:5538144-5538163 None:intergenic 45.0%
ATTGCTTTCATCCACTGCTC+TGG - Chr1:5539601-5539620 MsG0180000401.01.T01:intron 45.0%
CTGGGAAAGGAGATTTGAAG+CGG + Chr1:5536702-5536721 None:intergenic 45.0%
GATCCACTTCCAACTATGAC+AGG + Chr1:5538194-5538213 None:intergenic 45.0%
GCAGCTTGAATTCATAGCTC+TGG - Chr1:5537485-5537504 MsG0180000401.01.T01:intron 45.0%
GGAGCAATCCTTTATGGAAG+GGG - Chr1:5539655-5539674 MsG0180000401.01.T01:intron 45.0%
GGAGTTGGAGCAATCCTTTA+TGG - Chr1:5539649-5539668 MsG0180000401.01.T01:intron 45.0%
GGTTAGAGTGTACCTTAAGC+TGG + Chr1:5536721-5536740 None:intergenic 45.0%
GTTCTTCAAAGCATGGCAGT+AGG - Chr1:5538221-5538240 MsG0180000401.01.T01:intron 45.0%
GTTGTATGCCCCTTCCATAA+AGG + Chr1:5539666-5539685 None:intergenic 45.0%
TACCGTGCATCAGTGTAGTA+AGG + Chr1:5539237-5539256 None:intergenic 45.0%
TATCTGCAAACAAGGGTGGA+AGG + Chr1:5537872-5537891 None:intergenic 45.0%
TATTAAGCATGCCGTGGAGT+TGG - Chr1:5539634-5539653 MsG0180000401.01.T01:intron 45.0%
TCCAACTATGACAGGAGGTA+TGG + Chr1:5538186-5538205 None:intergenic 45.0%
TCCACCTAAACCAGACAGAA+AGG + Chr1:5538255-5538274 None:intergenic 45.0%
TCCACGGCATGCTTAATACA+AGG + Chr1:5539632-5539651 None:intergenic 45.0%
TCCATACCTCCTGTCATAGT+TGG - Chr1:5538182-5538201 MsG0180000401.01.T01:intron 45.0%
TCCTTGTATTAAGCATGCCG+TGG - Chr1:5539628-5539647 MsG0180000401.01.T01:intron 45.0%
TCTGCACAAGAAGTCTCAAC+AGG + Chr1:5539550-5539569 None:intergenic 45.0%
! ACCTTTCTGTCTGGTTTAGG+TGG - Chr1:5538251-5538270 MsG0180000401.01.T01:intron 45.0%
! CCGTTTAAGGCCTTTCTCAT+AGG + Chr1:5537590-5537609 None:intergenic 45.0%
! TTCCAAGAGTTCCTGGTCTT+TGG + Chr1:5540178-5540197 None:intergenic 45.0%
!! CACTTGCAGGTGTTACTAAC+AGG - Chr1:5537837-5537856 MsG0180000401.01.T01:intron 45.0%
!!! CTTTTATTAAAAAAAAAATA+CGG + Chr1:5538045-5538064 None:intergenic 5.0%
!!! TTTTATTAAAAAAAAAATAC+GGG + Chr1:5538044-5538063 None:intergenic 5.0%
ACAAGGATTCTCCAGAGCAG+TGG + Chr1:5539615-5539634 None:intergenic 50.0%
CACCAAAGACCAGGAACTCT+TGG - Chr1:5540173-5540192 MsG0180000401.01.T01:five_prime_UTR 50.0%
CAGGTTGTTGGGTGGCAATT+TGG - Chr1:5537360-5537379 MsG0180000401.01.T01:intron 50.0%
CCACTTCCAACTATGACAGG+AGG + Chr1:5538191-5538210 None:intergenic 50.0%
CCTCCTGTCATAGTTGGAAG+TGG - Chr1:5538188-5538207 MsG0180000401.01.T01:intron 50.0%
GATAGCAGGATGCAGTGAGA+AGG - Chr1:5540104-5540123 MsG0180000401.01.T01:CDS 50.0%
GGAAGACCAGAGCAACTGAT+TGG + Chr1:5538422-5538441 None:intergenic 50.0%
GGTGTATCTGCAAACAAGGG+TGG + Chr1:5537876-5537895 None:intergenic 50.0%
TCTGTCGAGCACTCAAGACA+CGG + Chr1:5539688-5539707 None:intergenic 50.0%
TGGGTACTCCTGAGGAGATT+GGG + Chr1:5539513-5539532 None:intergenic 50.0%
TTGGGTACTCCTGAGGAGAT+TGG + Chr1:5539514-5539533 None:intergenic 50.0%
TTTAGTGGCAGGTTGTTGGG+TGG - Chr1:5537352-5537371 MsG0180000401.01.T01:intron 50.0%
! GTTGCTGTTCACCAAAGACC+AGG - Chr1:5540164-5540183 MsG0180000401.01.T01:five_prime_UTR 50.0%
!! CCAAGTATTGGGTACTCCTG+AGG + Chr1:5539521-5539540 None:intergenic 50.0%
!! CCTCAGGAGTACCCAATACT+TGG - Chr1:5539518-5539537 MsG0180000401.01.T01:intron 50.0%
GGGTACTCCTGAGGAGATTG+GGG + Chr1:5539512-5539531 None:intergenic 55.0%
TCGTCTTCCCCAATCTCCTC+AGG - Chr1:5539502-5539521 MsG0180000401.01.T01:intron 55.0%
! ACAGAGGTGTGCGAGCTTCA+AGG + Chr1:5540011-5540030 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 5536546 5540233 5536546 ID=MsG0180000401.01;Name=MsG0180000401.01
Chr1 mRNA 5536546 5540233 5536546 ID=MsG0180000401.01.T01;Parent=MsG0180000401.01;Name=MsG0180000401.01.T01;_AED=0.44;_eAED=0.46;_QI=77|0.5|0.33|1|1|1|3|0|159
Chr1 exon 5540052 5540233 5540052 ID=MsG0180000401.01.T01:exon:2702;Parent=MsG0180000401.01.T01
Chr1 exon 5537017 5537255 5537017 ID=MsG0180000401.01.T01:exon:2703;Parent=MsG0180000401.01.T01
Chr1 exon 5536546 5536681 5536546 ID=MsG0180000401.01.T01:exon:2704;Parent=MsG0180000401.01.T01
Chr1 five_prime_UTR 5540157 5540233 5540157 ID=MsG0180000401.01.T01:five_prime_utr;Parent=MsG0180000401.01.T01
Chr1 CDS 5540052 5540156 5540052 ID=MsG0180000401.01.T01:cds;Parent=MsG0180000401.01.T01
Chr1 CDS 5537017 5537255 5537017 ID=MsG0180000401.01.T01:cds;Parent=MsG0180000401.01.T01
Chr1 CDS 5536546 5536681 5536546 ID=MsG0180000401.01.T01:cds;Parent=MsG0180000401.01.T01
Gene Sequence

>MsG0180000401.01.T01

ATGGATCCCAAATTCACAGAAGTTTCTCAGTTGTTCGATCGATTCAAAGCTGCTTTTCTCAGAAACGACTTCGATTCCGCTTCAAATCTCCTTTCCCAGCTTAAGGAGTACCCAATACTTGGTCTCAACCTGTTGAGACTTCTTGTGCAGAATAGGATTGCTGAATTCCATACTGAATTGGAATTGCTTTCATCCACTGCTCTGGAGAATCCTTGTATTAAGCATGCCGTGGAGTTGGAGCAATCCTTTATGGAAGGGGCATACAACCGTGTCTTGAGTGCTCGACAGACAGTACCACATGAAACATATGTGTATTTCATGGATCTTTTGGCAAAGACCATCAGAGATGAGATAGCAGGATGCAGTGAGAAGGCATATGACTATCTTTCAATTAATGATGCTAAGCAGATGTTGCTGTTCACCAAAGACCAGGAACTCTTGGAATATATTAAGGAGGTGAGAAATGTCACTGTACATTGA
Protein sequence

>MsG0180000401.01.T01

MDPKFTEVSQLFDRFKAAFLRNDFDSASNLLSQLKEYPILGLNLLRLLVQNRIAEFHTELELLSSTALENPCIKHAVELEQSFMEGAYNRVLSARQTVPHETYVYFMDLLAKTIRDEIAGCSEKAYDYLSINDAKQMLLFTKDQELLEYIKEVRNVTVH*