AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004510.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004510.01.T01 MTR_1g081420 67.901 162 13 3 1 126 1 159 7.84e-66 199
MsG0180004510.01.T01 MTR_1g081550 52.903 155 32 3 15 128 11 165 6.06e-45 144
MsG0180004510.01.T01 MTR_1g081450 80.220 91 3 1 1 76 1 91 1.32e-43 143
MsG0180004510.01.T01 MTR_1g081450 93.939 66 4 0 72 137 157 222 1.95e-36 124
MsG0180004510.01.T01 MTR_2g045280 47.500 160 43 3 12 130 39 198 3.49e-38 128
MsG0180004510.01.T01 MTR_4g124190 45.912 159 45 3 13 130 26 184 8.17e-37 124
MsG0180004510.01.T01 MTR_3g005755 50.725 138 29 3 13 128 75 195 2.21e-32 116
MsG0180004510.01.T01 MTR_1g081440 61.290 62 18 1 15 76 18 73 1.24e-19 78.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004510.01.T01 AT5G28050 42.500 160 51 3 12 130 24 183 1.13e-34 119
MsG0180004510.01.T01 AT5G28050 42.500 160 51 3 12 130 43 202 1.56e-34 119
MsG0180004510.01.T01 AT5G28050 42.500 160 51 3 12 130 129 288 6.87e-34 120

Find 21 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGGATTTAGTAATTATAT+TGG 0.292521 1:+78991831 MsG0180004510.01.T01:CDS
ATCAAGCAGAGAATGGTTTA+TGG 0.295789 1:+78991783 MsG0180004510.01.T01:intron
GCAGAAGCTGCAGGATCAAT+TGG 0.398102 1:+78991813 MsG0180004510.01.T01:CDS
CGAACAATCAGTGCACCATA+TGG 0.427952 1:-78990676 None:intergenic
GCATATGAAGCTGTTGAATC+TGG 0.453061 1:+78990643 MsG0180004510.01.T01:CDS
GCGGCTACTCCTGCTGCTAA+TGG 0.461836 1:+78990477 MsG0180004510.01.T01:CDS
TTTAGTAATTATATTGGCAA+GGG 0.530272 1:+78991837 MsG0180004510.01.T01:CDS
GAACAATCAGTGCACCATAT+GGG 0.543114 1:-78990675 None:intergenic
ATTTAGTAATTATATTGGCA+AGG 0.547598 1:+78991836 MsG0180004510.01.T01:CDS
GGAGCCACAGCAGAAGCTGC+AGG 0.548767 1:+78991804 MsG0180004510.01.T01:CDS
ATGATCTCAGCATGAGCAGT+TGG 0.550399 1:-78990751 None:intergenic
TTAGTATTACCATTAGCAGC+AGG 0.551637 1:-78990486 None:intergenic
GAAGACAAAGGACAAGGTAA+AGG 0.557065 1:+78991932 MsG0180004510.01.T01:CDS
TGATCCTGCAGCTTCTGCTG+TGG 0.574900 1:-78991808 None:intergenic
TCTGGTGATGGATACCCATA+TGG 0.575775 1:+78990661 MsG0180004510.01.T01:CDS
AGAAGTATTTGAGAAGACAA+AGG 0.606818 1:+78991920 MsG0180004510.01.T01:CDS
GAAGCTGTTGAATCTGGTGA+TGG 0.621594 1:+78990649 MsG0180004510.01.T01:CDS
TCTCGTTATCCCAGCTGCAG+AGG 0.636736 1:+78990582 MsG0180004510.01.T01:intron
CATCATGGAACATGATGCTG+CGG 0.653351 1:+78990458 None:intergenic
ATTTGAGAAGACAAAGGACA+AGG 0.678760 1:+78991926 MsG0180004510.01.T01:CDS
TGAGATCATTGCTATAAGAG+AGG 0.679141 1:+78990765 MsG0180004510.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATGTATGATTTTTTTTAA+TGG - Chr1:78990981-78991000 None:intergenic 10.0%
!! AATATAGTTCAAATTTCATA+AGG + Chr1:78991314-78991333 MsG0180004510.01.T01:intron 15.0%
!! ATATAGTTCAAATTTCATAA+GGG + Chr1:78991315-78991334 MsG0180004510.01.T01:intron 15.0%
!! ATTGGATTTAGTAATTATAT+TGG + Chr1:78991831-78991850 MsG0180004510.01.T01:CDS 15.0%
!!! AAAAAACACATTTTAACAAA+TGG - Chr1:78991123-78991142 None:intergenic 15.0%
!!! TATTCATTTTTCAAAAATCA+AGG + Chr1:78991673-78991692 MsG0180004510.01.T01:intron 15.0%
!! ATTCCAATTAGTATAACATA+AGG - Chr1:78991560-78991579 None:intergenic 20.0%
!! ATTTAGTAATTATATTGGCA+AGG + Chr1:78991836-78991855 MsG0180004510.01.T01:CDS 20.0%
!! TAAACAGAAATTATGATATC+TGG + Chr1:78991195-78991214 MsG0180004510.01.T01:intron 20.0%
!! TTACCTTATGTTATACTAAT+TGG + Chr1:78991554-78991573 MsG0180004510.01.T01:intron 20.0%
!! TTTAGTAATTATATTGGCAA+GGG + Chr1:78991837-78991856 MsG0180004510.01.T01:CDS 20.0%
!!! AATAGTTTGTTAAATGAAAG+AGG + Chr1:78991011-78991030 MsG0180004510.01.T01:intron 20.0%
!!! CTTCATATATTATTGCTTTT+TGG + Chr1:78990520-78990539 MsG0180004510.01.T01:intron 20.0%
!!! GACAATTTGATATTTTGAAT+AGG - Chr1:78991246-78991265 None:intergenic 20.0%
! AGAAATTATGATATCTGGAT+CGG + Chr1:78991200-78991219 MsG0180004510.01.T01:intron 25.0%
! ATTTGAATAACAAATTGAGG+AGG + Chr1:78991451-78991470 MsG0180004510.01.T01:intron 25.0%
! CACATTTGAATAACAAATTG+AGG + Chr1:78991448-78991467 MsG0180004510.01.T01:intron 25.0%
! TTCGAGATAAAAAAGATAGA+TGG + Chr1:78991876-78991895 MsG0180004510.01.T01:CDS 25.0%
!! AATACTTAGAAGTACATTTC+AGG - Chr1:78990561-78990580 None:intergenic 25.0%
!! ATAAGATTCTTCAGGTTATT+CGG - Chr1:78991375-78991394 None:intergenic 25.0%
!!! ATATTATTGCTTTTTGGTCA+AGG + Chr1:78990526-78990545 MsG0180004510.01.T01:intron 25.0%
!!! GTAATTCGACATTTTTTTGA+GGG + Chr1:78990836-78990855 MsG0180004510.01.T01:intron 25.0%
!!! TGTAATTCGACATTTTTTTG+AGG + Chr1:78990835-78990854 MsG0180004510.01.T01:intron 25.0%
AAGAATTTAGGGAGAAACAA+AGG - Chr1:78991155-78991174 None:intergenic 30.0%
AGTTCAAATTTCATAAGGGA+CGG + Chr1:78991319-78991338 MsG0180004510.01.T01:intron 30.0%
ATGATTGATATGGTTGAATG+AGG + Chr1:78991079-78991098 MsG0180004510.01.T01:intron 30.0%
ATGCCGAAGTAAAGAATTTA+GGG - Chr1:78991166-78991185 None:intergenic 30.0%
GTTTCCAACAATGATTGATA+TGG + Chr1:78991069-78991088 MsG0180004510.01.T01:intron 30.0%
TATGCCGAAGTAAAGAATTT+AGG - Chr1:78991167-78991186 None:intergenic 30.0%
TATTGTGAAGACAAAATGAG+AGG - Chr1:78991712-78991731 None:intergenic 30.0%
TCAACCATATCAATCATTGT+TGG - Chr1:78991076-78991095 None:intergenic 30.0%
TCTACAAGTTGTTAACTCAA+TGG + Chr1:78991264-78991283 MsG0180004510.01.T01:intron 30.0%
TCTCCCTAAATTCTTTACTT+CGG + Chr1:78991160-78991179 MsG0180004510.01.T01:intron 30.0%
TGATTGATATGGTTGAATGA+GGG + Chr1:78991080-78991099 MsG0180004510.01.T01:intron 30.0%
TTGTTAAATGAAAGAGGTCT+TGG + Chr1:78991017-78991036 MsG0180004510.01.T01:intron 30.0%
! TGCTGAAAGTTCATTTTGTT+TGG + Chr1:78991492-78991511 MsG0180004510.01.T01:intron 30.0%
!! AGAAGTATTTGAGAAGACAA+AGG + Chr1:78991920-78991939 MsG0180004510.01.T01:CDS 30.0%
ACTAATTGGAATTTACCTGC+AGG + Chr1:78991568-78991587 MsG0180004510.01.T01:intron 35.0%
ATATATGTCCATACAGCAGA+TGG - Chr1:78991399-78991418 None:intergenic 35.0%
ATATCTGGATCGGTATAATC+TGG + Chr1:78991210-78991229 MsG0180004510.01.T01:intron 35.0%
ATCAAGCAGAGAATGGTTTA+TGG + Chr1:78991783-78991802 MsG0180004510.01.T01:intron 35.0%
ATCATTGTTGGAAACCAAAG+TGG - Chr1:78991064-78991083 None:intergenic 35.0%
ATCTGCTGTATGGACATATA+TGG + Chr1:78991398-78991417 MsG0180004510.01.T01:intron 35.0%
ATCTTAATATCAGGCCACTT+TGG + Chr1:78991047-78991066 MsG0180004510.01.T01:intron 35.0%
ATCTTATACCATCTGCTGTA+TGG + Chr1:78991388-78991407 MsG0180004510.01.T01:intron 35.0%
ATTTGAGAAGACAAAGGACA+AGG + Chr1:78991926-78991945 MsG0180004510.01.T01:CDS 35.0%
CAGATGGTATAAGATTCTTC+AGG - Chr1:78991383-78991402 None:intergenic 35.0%
CTAAGTGATTAAAAGAGACG+AGG + Chr1:78990882-78990901 MsG0180004510.01.T01:intron 35.0%
GAATAACAAATTGAGGAGGT+TGG + Chr1:78991455-78991474 MsG0180004510.01.T01:intron 35.0%
TATTCACATCAAGCAGAGAA+TGG + Chr1:78991776-78991795 MsG0180004510.01.T01:intron 35.0%
TGAGATCATTGCTATAAGAG+AGG + Chr1:78990765-78990784 MsG0180004510.01.T01:CDS 35.0%
TTAGTATTACCATTAGCAGC+AGG - Chr1:78990489-78990508 None:intergenic 35.0%
! ATCTCAGTATCAGACAGTTT+TGG + Chr1:78991421-78991440 MsG0180004510.01.T01:intron 35.0%
! GTTAGTGTTTTCTCTTTGTG+AGG - Chr1:78990931-78990950 None:intergenic 35.0%
!! GGAGTGTTGATCTTAATATC+AGG + Chr1:78991038-78991057 MsG0180004510.01.T01:intron 35.0%
!!! TCGACATTTTTTTGAGGGTT+GGG + Chr1:78990841-78990860 MsG0180004510.01.T01:intron 35.0%
!!! TTCGACATTTTTTTGAGGGT+TGG + Chr1:78990840-78990859 MsG0180004510.01.T01:intron 35.0%
GAACAATCAGTGCACCATAT+GGG - Chr1:78990678-78990697 None:intergenic 40.0%
GAAGACAAAGGACAAGGTAA+AGG + Chr1:78991932-78991951 MsG0180004510.01.T01:CDS 40.0%
GATATGGTTGAATGAGGGAT+TGG + Chr1:78991085-78991104 MsG0180004510.01.T01:intron 40.0%
GCATATGAAGCTGTTGAATC+TGG + Chr1:78990643-78990662 MsG0180004510.01.T01:CDS 40.0%
TGAATAGCACCAAAGCACAT+TGG - Chr1:78991659-78991678 None:intergenic 40.0%
ATGATCTCAGCATGAGCAGT+TGG - Chr1:78990754-78990773 None:intergenic 45.0%
CGAACAATCAGTGCACCATA+TGG - Chr1:78990679-78990698 None:intergenic 45.0%
CTGGATAAAGTTTCCCTTGC+TGG + Chr1:78991602-78991621 MsG0180004510.01.T01:intron 45.0%
GAACCTTGTCCAATGTGCTT+TGG + Chr1:78991647-78991666 MsG0180004510.01.T01:intron 45.0%
GAAGCTGTTGAATCTGGTGA+TGG + Chr1:78990649-78990668 MsG0180004510.01.T01:CDS 45.0%
GACGAGGAGTTAAAAGAACG+TGG + Chr1:78990898-78990917 MsG0180004510.01.T01:intron 45.0%
GTACATTTCACAGCCAGCAA+GGG - Chr1:78991618-78991637 None:intergenic 45.0%
TGTACATTTCACAGCCAGCA+AGG - Chr1:78991619-78991638 None:intergenic 45.0%
!! TCTGGTGATGGATACCCATA+TGG + Chr1:78990661-78990680 MsG0180004510.01.T01:CDS 45.0%
CCTGCAGGCTTGTAAAAAGC+TGG + Chr1:78991583-78991602 MsG0180004510.01.T01:intron 50.0%
GCACCAAAGCACATTGGACA+AGG - Chr1:78991653-78991672 None:intergenic 50.0%
GCAGAAGCTGCAGGATCAAT+TGG + Chr1:78991813-78991832 MsG0180004510.01.T01:CDS 50.0%
TGTCTCTATCCTCTGCAGCT+GGG - Chr1:78990594-78990613 None:intergenic 50.0%
! CCAGCTTTTTACAAGCCTGC+AGG - Chr1:78991586-78991605 None:intergenic 50.0%
CTGTCTCTATCCTCTGCAGC+TGG - Chr1:78990595-78990614 None:intergenic 55.0%
TCTCGTTATCCCAGCTGCAG+AGG + Chr1:78990582-78990601 MsG0180004510.01.T01:intron 55.0%
TGATCCTGCAGCTTCTGCTG+TGG - Chr1:78991811-78991830 None:intergenic 55.0%
GCGGCTACTCCTGCTGCTAA+TGG + Chr1:78990477-78990496 MsG0180004510.01.T01:CDS 60.0%
GGAGCCACAGCAGAAGCTGC+AGG + Chr1:78991804-78991823 MsG0180004510.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 78990462 78991977 78990462 ID=MsG0180004510.01;Name=MsG0180004510.01
Chr1 mRNA 78990462 78991977 78990462 ID=MsG0180004510.01.T01;Parent=MsG0180004510.01;Name=MsG0180004510.01.T01;_AED=0.46;_eAED=0.48;_QI=0|0|0|1|1|1|3|0|137
Chr1 exon 78990462 78990498 78990462 ID=MsG0180004510.01.T01:exon:15266;Parent=MsG0180004510.01.T01
Chr1 exon 78990596 78990786 78990596 ID=MsG0180004510.01.T01:exon:15267;Parent=MsG0180004510.01.T01
Chr1 exon 78991792 78991977 78991792 ID=MsG0180004510.01.T01:exon:15268;Parent=MsG0180004510.01.T01
Chr1 CDS 78990462 78990498 78990462 ID=MsG0180004510.01.T01:cds;Parent=MsG0180004510.01.T01
Chr1 CDS 78990596 78990786 78990596 ID=MsG0180004510.01.T01:cds;Parent=MsG0180004510.01.T01
Chr1 CDS 78991792 78991977 78991792 ID=MsG0180004510.01.T01:cds;Parent=MsG0180004510.01.T01
Gene Sequence

>MsG0180004510.01.T01

ATGGAACATGATGCTGCGGCTACTCCTGCTGCTAATGCTGCAGAGGATAGAGACAGCAAATTCATAATAAAATCTGTTGAAGAAGCATATGAAGCTGTTGAATCTGGTGATGGATACCCATATGGTGCACTGATTGTTCGAAATGATGAAGTTATTGTAAGCTGTCATAACATGTTTTTAAGAAACAAAGAGCCAACTGCTCATGCTGAGATCATTGCTATAAGAGAGAGAATGGTTTATGGAGCCACAGCAGAAGCTGCAGGATCAATTGGATTTAGTAATTATATTGGCAAGGGCACTGATTTTCATAAGTTCGAGATAAAAAAGATAGATGGTATTGCTGCTGAAATTGCAGAAGAAGTATTTGAGAAGACAAAGGACAAGGTAAAGGCTAATCTTAAACGATCGCGTTAA

Protein sequence

>MsG0180004510.01.T01

MEHDAAATPAANAAEDRDSKFIIKSVEEAYEAVESGDGYPYGALIVRNDEVIVSCHNMFLRNKEPTAHAEIIAIRERMVYGATAEAAGSIGFSNYIGKGTDFHKFEIKKIDGIAAEIAEEVFEKTKDKVKANLKRSR*