AlfalfaGEDB | MsG0180003995.01

M. sativa cultivar ZhongmuNo.1 / MsG0180003995.01

KEGG: -
GO: GO:0003824 GO:0009058
PFAM: PF02567

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003995.01.T01	XP_003590670.1	70	100	5	1	1	100	3	77	2.73E-37	137

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003995.01.T01	Q9KG32	50.769	65	28	1	36	100	9	69	8.62E-14	67.8

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003995.01.T01	G7IEF2	70.000	100	5	1	1	100	3	77	1.31e-37	137

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180003361.01	MsG0180003995.01	0.824074	9.831215e-54	8.617994e-51
MsG0180003994.01	MsG0180003995.01	0.818450	1.948285e-52	1.459003e-49
MsG0180003995.01	MsG0180004143.01	0.801205	1.005158e-48	4.772054e-46
MsG0180003995.01	MsG0180004970.01	0.810041	1.403802e-50	8.374671e-48
MsG0180003995.01	MsG0280011034.01	0.807614	4.636959e-50	2.594480e-47
MsG0180003995.01	MsG0280011258.01	0.807155	5.804498e-50	3.209265e-47
MsG0180003995.01	MsG0380016140.01	0.802401	5.709614e-49	2.794311e-46
MsG0180003995.01	MsG0480019710.01	0.807860	4.111721e-50	2.315399e-47
MsG0180003995.01	MsG0580024489.01	0.830637	2.624928e-55	2.781794e-52
MsG0180003995.01	MsG0580025614.01	0.806922	6.501970e-50	3.573216e-47
MsG0180003995.01	MsG0580028176.01	0.800925	1.146510e-48	5.405005e-46
MsG0180003995.01	MsG0680030949.01	0.827061	1.926239e-54	1.839213e-51
MsG0180003995.01	MsG0780036752.01	0.803667	3.124479e-49	1.579351e-46
MsG0180003995.01	MsG0880041860.01	0.824041	1.000483e-53	8.761729e-51
MsG0180003995.01	MsG0880042961.01	0.807697	4.454243e-50	2.497603e-47
MsG0180003995.01	MsG0880043516.01	0.807774	4.289958e-50	2.410368e-47
MsG0180003995.01	MsG0880047226.01	0.831714	1.427075e-55	1.561576e-52
MsG0180003995.01	MsG0880047666.01	0.802402	5.706874e-49	2.793047e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003995.01.T01	MTR_1g072470	70.000	100	5	1	1	100	3	77	3.31e-41	137
MsG0180003995.01.T01	MTR_1g062500	58.000	100	17	1	1	100	1	75	1.05e-32	115
MsG0180003995.01.T01	MTR_1g062530	76.923	65	15	0	36	100	11	75	1.48e-32	113
MsG0180003995.01.T01	MTR_1g072500	57.000	100	18	1	1	100	1	75	3.10e-32	114
MsG0180003995.01.T01	MTR_1g062540	57.843	102	16	2	1	100	1	77	4.25e-32	114
MsG0180003995.01.T01	MTR_1g072490	56.000	100	19	1	1	100	1	75	2.23e-31	110
MsG0180003995.01.T01	MTR_4g107220	58.000	100	16	2	1	99	1	75	6.25e-31	110
MsG0180003995.01.T01	MTR_1g072490	56.000	100	19	1	1	100	1	75	1.26e-30	110

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003995.01.T01	AT4G02850	61.194	67	24	1	36	100	14	80	1.83e-24	94.4
MsG0180003995.01.T01	AT1G03210	67.164	67	19	2	36	100	12	77	2.25e-24	94.0
MsG0180003995.01.T01	AT1G03210	67.164	67	19	2	36	100	12	77	4.81e-24	92.8
MsG0180003995.01.T01	AT4G02860	65.672	67	20	2	36	100	31	96	5.06e-24	93.2

Find 18 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTGACAGGAATCAAATAAC+AGG	0.382844	1:+71522644	None:intergenic
AAACCATCTAAGACGAAAAC+GGG	0.395279	1:+71522608	None:intergenic
TCTAACAAACACACTGCTGC+AGG	0.436665	1:+71522726	None:intergenic
GGATTGGAGGTTCCTTTGAC+AGG	0.467184	1:+71522630	None:intergenic
CTCACCACATAGTATTTCAC+TGG	0.469841	1:+71522855	None:intergenic
TCTAAGACGAAAACGGGGAT+TGG	0.481220	1:+71522614	None:intergenic
TAAACCATCTAAGACGAAAA+CGG	0.482204	1:+71522607	None:intergenic
TTTACTGAGTTGGCGTTCAA+GGG	0.488387	1:-71522753	MsG0180003995.01.T01:CDS
AACAAGAGAAGGACGATGAA+TGG	0.491833	1:-71522704	MsG0180003995.01.T01:CDS
AAGACGAAAACGGGGATTGG+AGG	0.545421	1:+71522617	None:intergenic
TGTTATTTGATTCCTGTCAA+AGG	0.547040	1:-71522642	MsG0180003995.01.T01:CDS
TAATGCTGATGTTATTACAC+AGG	0.557294	1:-71522784	MsG0180003995.01.T01:CDS
ATGGTTTACTCATATTGTTG+AGG	0.608452	1:-71522592	MsG0180003995.01.T01:CDS
TTACTGAGTTGGCGTTCAAG+GGG	0.614038	1:-71522752	MsG0180003995.01.T01:CDS
GTGTTTGTTAGAACAAGAGA+AGG	0.635175	1:-71522715	MsG0180003995.01.T01:CDS
TAAACCAGTGAAATACTATG+TGG	0.642454	1:-71522859	MsG0180003995.01.T01:CDS
CTAACAAACACACTGCTGCA+GGG	0.643974	1:+71522727	None:intergenic
AACCATCTAAGACGAAAACG+GGG	0.726239	1:+71522609	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATGGTTTACTCATATTGTTG+AGG	-	Chr1:71522862-71522881	MsG0180003995.01.T01:CDS	30.0%
	TAAACCAGTGAAATACTATG+TGG	-	Chr1:71522595-71522614	MsG0180003995.01.T01:CDS	30.0%
	TAATGCTGATGTTATTACAC+AGG	-	Chr1:71522670-71522689	MsG0180003995.01.T01:CDS	30.0%
	TTTGACAGGAATCAAATAAC+AGG	+	Chr1:71522813-71522832	None:intergenic	30.0%
!	TAAACCATCTAAGACGAAAA+CGG	+	Chr1:71522850-71522869	None:intergenic	30.0%
!	TGTTATTTGATTCCTGTCAA+AGG	-	Chr1:71522812-71522831	MsG0180003995.01.T01:CDS	30.0%
!	AAACCATCTAAGACGAAAAC+GGG	+	Chr1:71522849-71522868	None:intergenic	35.0%
!	GTGTTTGTTAGAACAAGAGA+AGG	-	Chr1:71522739-71522758	MsG0180003995.01.T01:CDS	35.0%
	AACAAGAGAAGGACGATGAA+TGG	-	Chr1:71522750-71522769	MsG0180003995.01.T01:CDS	40.0%
	CTCACCACATAGTATTTCAC+TGG	+	Chr1:71522602-71522621	None:intergenic	40.0%
!	AACCATCTAAGACGAAAACG+GGG	+	Chr1:71522848-71522867	None:intergenic	40.0%
!	TTTACTGAGTTGGCGTTCAA+GGG	-	Chr1:71522701-71522720	MsG0180003995.01.T01:CDS	40.0%
!!	GGTTGATGCTTTTACTGAGT+TGG	-	Chr1:71522691-71522710	MsG0180003995.01.T01:CDS	40.0%
!!	TTTTACTGAGTTGGCGTTCA+AGG	-	Chr1:71522700-71522719	MsG0180003995.01.T01:CDS	40.0%
	CTAACAAACACACTGCTGCA+GGG	+	Chr1:71522730-71522749	None:intergenic	45.0%
	TCTAACAAACACACTGCTGC+AGG	+	Chr1:71522731-71522750	None:intergenic	45.0%
!	ATCCCCGTTTTCGTCTTAGA+TGG	-	Chr1:71522843-71522862	MsG0180003995.01.T01:CDS	45.0%
!	TCTAAGACGAAAACGGGGAT+TGG	+	Chr1:71522843-71522862	None:intergenic	45.0%
!	TTACTGAGTTGGCGTTCAAG+GGG	-	Chr1:71522702-71522721	MsG0180003995.01.T01:CDS	45.0%
	AAGACGAAAACGGGGATTGG+AGG	+	Chr1:71522840-71522859	None:intergenic	50.0%
	GGATTGGAGGTTCCTTTGAC+AGG	+	Chr1:71522827-71522846	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	71522587	71522889	71522587	ID=MsG0180003995.01;Name=MsG0180003995.01
Chr1	mRNA	71522587	71522889	71522587	ID=MsG0180003995.01.T01;Parent=MsG0180003995.01;Name=MsG0180003995.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|100
Chr1	exon	71522587	71522889	71522587	ID=MsG0180003995.01.T01:exon:30875;Parent=MsG0180003995.01.T01
Chr1	CDS	71522587	71522889	71522587	ID=MsG0180003995.01.T01:cds;Parent=MsG0180003995.01.T01

Gene Sequence	>MsG0180003995.01.T01 ATGTCAAATAAACCAGTGAAATACTATGTGGTGAGTTTATTTTGTTTGAATGTGAACTTTGTATTCATTGATGAATTAGTTAATAATGCTGATGTTATTACACAGGTTGATGCTTTTACTGAGTTGGCGTTCAAGGGGAACCCTGCAGCAGTGTGTTTGTTAGAACAAGAGAAGGACGATGAATGGTTGCAAGCATTAGCAGCTGAGTTCAATATCAATGTCACCTGTTATTTGATTCCTGTCAAAGGAACCTCCAATCCCCGTTTTCGTCTTAGATGGTTTACTCATATTGTTGAGGTATAG
Protein sequence	>MsG0180003995.01.T01 MSNKPVKYYVVSLFCLNVNFVFIDELVNNADVITQVDAFTELAFKGNPAAVCLLEQEKDDEWLQALAAEFNINVTCYLIPVKGTSNPRFRLRWFTHIVEV*