AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003994.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003994.01.T01 MTR_1g072470 93.805 226 14 0 5 230 74 299 4.90e-157 437
MsG0180003994.01.T01 MTR_4g107220 74.009 227 57 2 5 230 73 298 3.45e-123 351
MsG0180003994.01.T01 MTR_1g072500 77.434 226 46 3 5 230 72 292 4.90e-123 351
MsG0180003994.01.T01 MTR_1g062540 74.449 227 56 2 6 230 75 301 5.16e-122 348
MsG0180003994.01.T01 MTR_1g072490 73.894 226 55 3 5 230 72 293 3.26e-120 343
MsG0180003994.01.T01 MTR_1g062500 71.366 227 61 4 6 230 73 297 1.13e-112 325
MsG0180003994.01.T01 MTR_1g072490 65.644 163 52 3 5 167 72 230 1.54e-70 215
MsG0180003994.01.T01 MTR_1g062440 73.228 127 34 0 104 230 57 183 2.28e-66 203
MsG0180003994.01.T01 MTR_1g062530 66.061 165 52 4 5 167 72 234 3.76e-66 204
MsG0180003994.01.T01 MTR_1g062510 88.679 53 6 0 178 230 14 66 8.99e-29 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003994.01.T01 AT1G03210 53.947 228 88 5 5 230 74 286 3.70e-79 239
MsG0180003994.01.T01 AT4G02860 52.402 229 98 5 5 230 93 313 5.31e-78 237
MsG0180003994.01.T01 AT1G03210 46.850 254 94 4 5 230 74 314 3.38e-73 225
MsG0180003994.01.T01 AT4G02850 45.455 242 104 2 5 230 77 306 2.07e-71 220

Find 46 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTCATTGACTCCAAATTT+TGG 0.097056 1:+71517776 None:intergenic
TTAACAATTACATCAAATTT+TGG 0.174266 1:+71517878 None:intergenic
TCGAATTTGAACTCTGTAAT+AGG 0.182305 1:+71518155 None:intergenic
TGTCCTCTTTATATCAATTA+TGG 0.209150 1:+71518094 None:intergenic
ACTAGGGAAGTGTGATTTAA+AGG 0.229073 1:-71517573 MsG0180003994.01.T01:CDS
ATTGAATTTGCGACACTATC+TGG 0.230222 1:-71518290 MsG0180003994.01.T01:CDS
GTGTGATTTAAAGGCTTATC+AGG 0.308479 1:-71517564 MsG0180003994.01.T01:intron
GGATGCATCTTATATTGAAT+TGG 0.326398 1:-71518192 MsG0180003994.01.T01:CDS
TGCACTCTTTGCATCTGGTT+TGG 0.350466 1:-71518330 MsG0180003994.01.T01:CDS
TATAAAGAGGACACAAATTC+AGG 0.350811 1:-71518084 MsG0180003994.01.T01:CDS
GATTTGAAGAATTTGCAAAA+TGG 0.352659 1:-71518224 MsG0180003994.01.T01:CDS
TCACATGCACTCTTTGCATC+TGG 0.361453 1:-71518335 MsG0180003994.01.T01:CDS
CCCTAGTTTCTTGCTCCAAT+AGG 0.378188 1:+71517589 None:intergenic
TTCACTTATTTCTCAAGCAT+TGG 0.393639 1:-71518126 MsG0180003994.01.T01:CDS
CACATCAGATACAGAATGTT+AGG 0.398818 1:+71517328 None:intergenic
ATTGTGGATTGGCATCCTAT+TGG 0.402709 1:-71517604 MsG0180003994.01.T01:CDS
CGAATTTGAACTCTGTAATA+GGG 0.410851 1:+71518156 None:intergenic
TCCTAGGTTGTAGTCACATC+AGG 0.446974 1:-71517923 MsG0180003994.01.T01:intron
TCTGGATCATATTATAATAC+AGG 0.449544 1:-71518390 MsG0180003994.01.T01:CDS
AGTGATGGAAGGATGTGTTT+TGG 0.449987 1:-71517363 MsG0180003994.01.T01:CDS
CAATGTGCACTCCCACAAAC+AGG 0.466512 1:+71517623 None:intergenic
AACTCTGTAATAGGGTCAGC+AGG 0.471059 1:+71518164 None:intergenic
CTTATTTCTCAAGCATTGGA+TGG 0.479686 1:-71518122 MsG0180003994.01.T01:CDS
GGGATTGCTCCTCCAGAGTC+GGG 0.485744 1:-71517827 MsG0180003994.01.T01:CDS
TGGGAGTGCACATTGTGGAT+TGG 0.496288 1:-71517615 MsG0180003994.01.T01:CDS
GATGTAATTGTTAAATGTCC+TGG 0.498911 1:-71517869 MsG0180003994.01.T01:CDS
TAGAAATCAAATCCCGACTC+TGG 0.499245 1:+71517815 None:intergenic
TCCTATTGGAGCAAGAAACT+AGG 0.499747 1:-71517590 MsG0180003994.01.T01:CDS
CTTCCATAATTGATATAAAG+AGG 0.515089 1:-71518097 MsG0180003994.01.T01:CDS
ATTGTTAAATGTCCTGGAAG+GGG 0.544587 1:-71517863 MsG0180003994.01.T01:CDS
TTTGCAAAATGGTAAAGCTA+AGG 0.557399 1:-71518213 MsG0180003994.01.T01:CDS
TAATTGTTAAATGTCCTGGA+AGG 0.564168 1:-71517865 MsG0180003994.01.T01:CDS
CCTATTGGAGCAAGAAACTA+GGG 0.566460 1:-71517589 MsG0180003994.01.T01:CDS
AGGGATTGCTCCTCCAGAGT+CGG 0.567221 1:-71517828 MsG0180003994.01.T01:CDS
CCCAAAATTTGGAGTCAATG+AGG 0.570026 1:-71517777 MsG0180003994.01.T01:intron
TGGGAAGGCTATTACAGTGA+TGG 0.573234 1:-71517378 MsG0180003994.01.T01:CDS
AATTGTTAAATGTCCTGGAA+GGG 0.577074 1:-71517864 MsG0180003994.01.T01:CDS
GTGAAGCTGCTAATGTGGCA+TGG 0.580569 1:+71518354 None:intergenic
TTTGTTCAGGCATCAGCTAG+AGG 0.605347 1:-71517452 MsG0180003994.01.T01:intron
TGAAGCTGCTAATGTGGCAT+GGG 0.609399 1:+71518355 None:intergenic
GTTTGTGGGAGTGCACATTG+TGG 0.618544 1:-71517620 MsG0180003994.01.T01:CDS
AAGGCTATTACAGTGATGGA+AGG 0.629492 1:-71517374 MsG0180003994.01.T01:CDS
TTGTTAAATGTCCTGGAAGG+GGG 0.633343 1:-71517862 MsG0180003994.01.T01:CDS
GTTCAGGCATCAGCTAGAGG+AGG 0.652622 1:-71517449 MsG0180003994.01.T01:intron
TGCATGTGAAGCTGCTAATG+TGG 0.657511 1:+71518349 None:intergenic
AAATCAAATCCCGACTCTGG+AGG 0.679685 1:+71517818 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGAAATAAAAAAGCATATT+AGG + Chr1:71517689-71517708 None:intergenic 15.0%
!!! TTAACAATTACATCAAATTT+TGG + Chr1:71517855-71517874 None:intergenic 15.0%
!! AAAAAAATCTCAATGATCAA+TGG - Chr1:71517476-71517495 MsG0180003994.01.T01:intron 20.0%
!! CAAAAAACAACAAAAGTAAA+CGG + Chr1:71517750-71517769 None:intergenic 20.0%
!!! AGTAACTCATTTTTCTTTTT+AGG - Chr1:71518082-71518101 MsG0180003994.01.T01:CDS 20.0%
!!! ATTGATCATTGAGATTTTTT+TGG + Chr1:71517477-71517496 None:intergenic 20.0%
! AGATTGCAATATATTTGTTC+AGG - Chr1:71518265-71518284 MsG0180003994.01.T01:CDS 25.0%
! CTTCCATAATTGATATAAAG+AGG - Chr1:71517633-71517652 MsG0180003994.01.T01:intron 25.0%
! GATAATAAGCTTCATAATGT+TGG + Chr1:71517782-71517801 None:intergenic 25.0%
! GATTTGAAGAATTTGCAAAA+TGG - Chr1:71517506-71517525 MsG0180003994.01.T01:intron 25.0%
! TGTCCTCTTTATATCAATTA+TGG + Chr1:71517639-71517658 None:intergenic 25.0%
AATTGTTAAATGTCCTGGAA+GGG - Chr1:71517866-71517885 MsG0180003994.01.T01:CDS 30.0%
CCTGTATTATAATATGATCC+AGG + Chr1:71517343-71517362 None:intergenic 30.0%
CGAATTTGAACTCTGTAATA+GGG + Chr1:71517577-71517596 None:intergenic 30.0%
CTTCATTCATTGCATAGTTA+AGG + Chr1:71518028-71518047 None:intergenic 30.0%
GACAAAGATAATCACAATGA+GGG + Chr1:71518227-71518246 None:intergenic 30.0%
GATGTAATTGTTAAATGTCC+TGG - Chr1:71517861-71517880 MsG0180003994.01.T01:CDS 30.0%
GGATGCATCTTATATTGAAT+TGG - Chr1:71517538-71517557 MsG0180003994.01.T01:intron 30.0%
TAATTGTTAAATGTCCTGGA+AGG - Chr1:71517865-71517884 MsG0180003994.01.T01:CDS 30.0%
TATAAAGAGGACACAAATTC+AGG - Chr1:71517646-71517665 MsG0180003994.01.T01:intron 30.0%
TCGAATTTGAACTCTGTAAT+AGG + Chr1:71517578-71517597 None:intergenic 30.0%
TGACAAAGATAATCACAATG+AGG + Chr1:71518228-71518247 None:intergenic 30.0%
TGTGATTATCTTTGTCATTG+TGG - Chr1:71518231-71518250 MsG0180003994.01.T01:CDS 30.0%
TTTGCAAAATGGTAAAGCTA+AGG - Chr1:71517517-71517536 MsG0180003994.01.T01:intron 30.0%
! TTCACTTATTTCTCAAGCAT+TGG - Chr1:71517604-71517623 MsG0180003994.01.T01:CDS 30.0%
ACCATCACGAATGATATCTT+AGG - Chr1:71517723-71517742 MsG0180003994.01.T01:intron 35.0%
AGCTTATTATCATCTCTCCT+AGG - Chr1:71517791-71517810 MsG0180003994.01.T01:CDS 35.0%
ATTGAATTTGCGACACTATC+TGG - Chr1:71517440-71517459 MsG0180003994.01.T01:CDS 35.0%
ATTGTTAAATGTCCTGGAAG+GGG - Chr1:71517867-71517886 MsG0180003994.01.T01:CDS 35.0%
GTGTGATTTAAAGGCTTATC+AGG - Chr1:71518166-71518185 MsG0180003994.01.T01:CDS 35.0%
TCCTAAGATATCATTCGTGA+TGG + Chr1:71517727-71517746 None:intergenic 35.0%
! ACTAGGGAAGTGTGATTTAA+AGG - Chr1:71518157-71518176 MsG0180003994.01.T01:CDS 35.0%
! CGATTTTTCTGCCCAAAATT+TGG - Chr1:71517942-71517961 MsG0180003994.01.T01:intron 35.0%
!! CTTATTTCTCAAGCATTGGA+TGG - Chr1:71517608-71517627 MsG0180003994.01.T01:CDS 35.0%
!!! AAACAAAGAGTGTTTTTGCG+TGG - Chr1:71518332-71518351 MsG0180003994.01.T01:CDS 35.0%
!!! AACAAAGAGTGTTTTTGCGT+GGG - Chr1:71518333-71518352 MsG0180003994.01.T01:CDS 35.0%
!!! ACCTCATTGACTCCAAATTT+TGG + Chr1:71517957-71517976 None:intergenic 35.0%
!!! CCTCATTGACTCCAAATTTT+GGG + Chr1:71517956-71517975 None:intergenic 35.0%
!!! CTTTTTAGGATCCTGTTTGT+GGG - Chr1:71518096-71518115 MsG0180003994.01.T01:CDS 35.0%
!!! TCTTTTTAGGATCCTGTTTG+TGG - Chr1:71518095-71518114 MsG0180003994.01.T01:CDS 35.0%
CCTATTGGAGCAAGAAACTA+GGG - Chr1:71518141-71518160 MsG0180003994.01.T01:CDS 40.0%
GAAGGGGGATAATTGTTACA+GGG - Chr1:71517883-71517902 MsG0180003994.01.T01:CDS 40.0%
TAGAAATCAAATCCCGACTC+TGG + Chr1:71517918-71517937 None:intergenic 40.0%
TCCTATTGGAGCAAGAAACT+AGG - Chr1:71518140-71518159 MsG0180003994.01.T01:CDS 40.0%
TTGTTAAATGTCCTGGAAGG+GGG - Chr1:71517868-71517887 MsG0180003994.01.T01:CDS 40.0%
! AAGGCTATTACAGTGATGGA+AGG - Chr1:71518356-71518375 MsG0180003994.01.T01:CDS 40.0%
! CCCAAAATTTGGAGTCAATG+AGG - Chr1:71517953-71517972 MsG0180003994.01.T01:intron 40.0%
!! AGTGATGGAAGGATGTGTTT+TGG - Chr1:71518367-71518386 MsG0180003994.01.T01:CDS 40.0%
!! ATTGTGGATTGGCATCCTAT+TGG - Chr1:71518126-71518145 MsG0180003994.01.T01:CDS 40.0%
AAATCAAATCCCGACTCTGG+AGG + Chr1:71517915-71517934 None:intergenic 45.0%
AACTCTGTAATAGGGTCAGC+AGG + Chr1:71517569-71517588 None:intergenic 45.0%
CCCTAGTTTCTTGCTCCAAT+AGG + Chr1:71518144-71518163 None:intergenic 45.0%
GGAAGGGGGATAATTGTTAC+AGG - Chr1:71517882-71517901 MsG0180003994.01.T01:CDS 45.0%
GTAACAATTATCCCCCTTCC+AGG + Chr1:71517882-71517901 None:intergenic 45.0%
TCACATGCACTCTTTGCATC+TGG - Chr1:71517395-71517414 MsG0180003994.01.T01:CDS 45.0%
TCCTAGGTTGTAGTCACATC+AGG - Chr1:71517807-71517826 MsG0180003994.01.T01:CDS 45.0%
TCCTGATGTGACTACAACCT+AGG + Chr1:71517811-71517830 None:intergenic 45.0%
TGAAGCTGCTAATGTGGCAT+GGG + Chr1:71517378-71517397 None:intergenic 45.0%
TGCACTCTTTGCATCTGGTT+TGG - Chr1:71517400-71517419 MsG0180003994.01.T01:CDS 45.0%
TGCATGTGAAGCTGCTAATG+TGG + Chr1:71517384-71517403 None:intergenic 45.0%
TGGGAAGGCTATTACAGTGA+TGG - Chr1:71518352-71518371 MsG0180003994.01.T01:CDS 45.0%
TTTGTTCAGGCATCAGCTAG+AGG - Chr1:71518278-71518297 MsG0180003994.01.T01:CDS 45.0%
!!! AAGAGTGTTTTTGCGTGGGA+AGG - Chr1:71518337-71518356 MsG0180003994.01.T01:CDS 45.0%
CAATGTGCACTCCCACAAAC+AGG + Chr1:71518110-71518129 None:intergenic 50.0%
GTGAAGCTGCTAATGTGGCA+TGG + Chr1:71517379-71517398 None:intergenic 50.0%
! GTTTGTGGGAGTGCACATTG+TGG - Chr1:71518110-71518129 MsG0180003994.01.T01:CDS 50.0%
! TGGGAGTGCACATTGTGGAT+TGG - Chr1:71518115-71518134 MsG0180003994.01.T01:CDS 50.0%
AGGGATTGCTCCTCCAGAGT+CGG - Chr1:71517902-71517921 MsG0180003994.01.T01:CDS 55.0%
GTTCAGGCATCAGCTAGAGG+AGG - Chr1:71518281-71518300 MsG0180003994.01.T01:CDS 55.0%
GGGATTGCTCCTCCAGAGTC+GGG - Chr1:71517903-71517922 MsG0180003994.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 71517341 71518411 71517341 ID=MsG0180003994.01;Name=MsG0180003994.01
Chr1 mRNA 71517341 71518411 71517341 ID=MsG0180003994.01.T01;Parent=MsG0180003994.01;Name=MsG0180003994.01.T01;_AED=0.48;_eAED=0.48;_QI=0|1|0.75|1|1|1|4|17|230
Chr1 exon 71517341 71517465 71517341 ID=MsG0180003994.01.T01:exon:30874;Parent=MsG0180003994.01.T01
Chr1 exon 71517565 71517648 71517565 ID=MsG0180003994.01.T01:exon:30873;Parent=MsG0180003994.01.T01
Chr1 exon 71517778 71517939 71517778 ID=MsG0180003994.01.T01:exon:30872;Parent=MsG0180003994.01.T01
Chr1 exon 71518073 71518411 71518073 ID=MsG0180003994.01.T01:exon:30871;Parent=MsG0180003994.01.T01
Chr1 CDS 71518073 71518411 71518073 ID=MsG0180003994.01.T01:cds;Parent=MsG0180003994.01.T01
Chr1 CDS 71517778 71517939 71517778 ID=MsG0180003994.01.T01:cds;Parent=MsG0180003994.01.T01
Chr1 CDS 71517565 71517648 71517565 ID=MsG0180003994.01.T01:cds;Parent=MsG0180003994.01.T01
Chr1 CDS 71517358 71517465 71517358 ID=MsG0180003994.01.T01:cds;Parent=MsG0180003994.01.T01
Chr1 three_prime_UTR 71517341 71517357 71517341 ID=MsG0180003994.01.T01:three_prime_utr;Parent=MsG0180003994.01.T01
Gene Sequence

>MsG0180003994.01.T01

CTGGATCATATTATAATACAGGTTAATATTTGTGCCCATGCCACATTAGCAGCTTCACATGCACTCTTTGCATCTGGTTTGGTGAACAACAATGTTACTATTGAATTTGCGACACTATCTGGAATATTAACTGCCAAAAAAATCTCAATGATCAATGGCACTAGTGATTTGAAGAATTTGCAAAATGGTAAAGCTAAGGATGCATCTTATATTGAATTGGATTTTCCTGCTGACCCTATTACAGAGTTCAAATTCGATGATACTTCACTTATTTCTCAAGCATTGGATGGAGCTTCCATAATTGATATAAAGAGGACACAAATTCAGGATGACTTACTTGTTGTAGTCACATCAGGAAAGAATGTCAGAGAAGTGCAACCAAAATTTGATGTAATTGTTAAATGTCCTGGAAGGGGGATAATTGTTACAGGGATTGCTCCTCCAGAGTCGGGATTTGATTTCTATAGTCGATTTTTCTGCCCAAAATTTGGAGTCAATGAGGATCCTGTTTGTGGGAGTGCACATTGTGGATTGGCATCCTATTGGAGCAAGAAACTAGGGAAGTGTGATTTAAAGGCTTATCAGGCATCAGCTAGAGGAGGAATTCTGAATATTCATATTGATGAACAGAAACAAAGAGTGTTTTTGCGTGGGAAGGCTATTACAGTGATGGAAGGATGTGTTTTGGTCTAG

Protein sequence

>MsG0180003994.01.T01

LDHIIIQVNICAHATLAASHALFASGLVNNNVTIEFATLSGILTAKKISMINGTSDLKNLQNGKAKDASYIELDFPADPITEFKFDDTSLISQALDGASIIDIKRTQIQDDLLVVVTSGKNVREVQPKFDVIVKCPGRGIIVTGIAPPESGFDFYSRFFCPKFGVNEDPVCGSAHCGLASYWSKKLGKCDLKAYQASARGGILNIHIDEQKQRVFLRGKAITVMEGCVLV*