AlfalfaGEDB | MsG0280011034.01

M. sativa cultivar ZhongmuNo.1 / MsG0280011034.01

KEGG: -
GO: GO:0004252 GO:0016021
PFAM: PF01694

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011034.01.T01	XP_003597357.1	82.812	384	14	3	1	333	1	383	0	589

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011034.01.T01	Q0WQX7	50.794	378	132	4	6	332	15	389	4.68E-132	384

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011034.01.T01	G7IHP5	82.812	384	14	3	1	333	1	383	0.0	589

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048700.01	MsG0280011034.01	0.802994	4.307614e-49	2.140063e-46
MsG0080048829.01	MsG0280011034.01	0.806869	6.672376e-50	3.661885e-47
MsG0080049140.01	MsG0280011034.01	0.802761	4.812279e-49	2.376831e-46
MsG0180000195.01	MsG0280011034.01	0.801928	7.142945e-49	3.453790e-46
MsG0180000300.01	MsG0280011034.01	0.805300	1.426534e-49	7.520397e-47
MsG0180000353.01	MsG0280011034.01	0.825586	4.323680e-54	3.957615e-51
MsG0180000360.01	MsG0280011034.01	0.818107	2.329540e-52	1.727843e-49
MsG0180001565.01	MsG0280011034.01	0.825699	4.064771e-54	3.732729e-51
MsG0180003943.01	MsG0280011034.01	0.811504	6.774732e-51	4.201050e-48
MsG0180003995.01	MsG0280011034.01	0.807614	4.636959e-50	2.594480e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011034.01.T01	MTR_2g097210	82.812	384	14	3	1	333	1	383	0.0	589
MsG0280011034.01.T01	MTR_2g097210	79.070	301	11	3	1	250	1	300	2.35e-165	462
MsG0280011034.01.T01	MTR_4g021755	50.327	306	98	3	37	291	34	336	2.88e-109	324
MsG0280011034.01.T01	MTR_7g498280	47.647	340	111	5	1	287	1	326	1.41e-86	263
MsG0280011034.01.T01	MTR_7g061780	47.541	305	103	4	23	275	4	303	2.92e-86	263
MsG0280011034.01.T01	MTR_1g101310	42.249	329	120	5	16	288	5	319	1.45e-74	233
MsG0280011034.01.T01	MTR_3g073530	32.891	377	189	7	13	331	9	379	2.09e-66	214
MsG0280011034.01.T01	MTR_2g103600	41.455	275	102	4	44	261	30	302	7.04e-63	202
MsG0280011034.01.T01	MTR_2g088290	32.114	246	118	8	65	289	1	218	8.03e-26	103

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011034.01.T01	AT2G29050	50.794	378	132	4	6	332	15	389	4.78e-133	384
MsG0280011034.01.T01	AT3G53780	49.570	349	117	4	35	332	54	394	1.41e-110	327
MsG0280011034.01.T01	AT1G63120	50.340	294	87	4	44	284	30	317	8.80e-88	266
MsG0280011034.01.T01	AT5G07250	48.171	328	104	5	21	286	22	345	1.49e-84	259
MsG0280011034.01.T01	AT1G12750	43.934	305	115	4	33	284	6	307	5.44e-82	251
MsG0280011034.01.T01	AT1G12750	43.934	305	115	4	33	284	6	307	5.44e-82	251
MsG0280011034.01.T01	AT1G12750	43.934	305	115	4	33	284	6	307	5.44e-82	251
MsG0280011034.01.T01	AT1G12750	43.934	305	115	4	33	284	6	307	5.44e-82	251
MsG0280011034.01.T01	AT1G12750	43.934	305	115	4	33	284	6	307	5.44e-82	251
MsG0280011034.01.T01	AT3G53780	47.464	276	88	3	108	332	1	270	2.73e-81	248

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Find 100 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTCACGTAAAGGAAGA+AGG	0.547703	2:+78694990	None:intergenic
AAAATCATAATCATGAGTGG+AGG	0.633390	2:-78695299	MsG0280011034.01.T01:exon
AAAATCATCTATAATCACGG+AGG	0.711965	2:-78695398	MsG0280011034.01.T01:five_prime_UTR
AAACAATTCTCCACAGACAA+AGG	0.590975	2:-78695455	MsG0280011034.01.T01:five_prime_UTR
AAAGTGGTTCATGAACACGA+AGG	0.793505	2:-78694079	MsG0280011034.01.T01:CDS
AAATCACGATCACGAGTGAC+GGG	0.541550	2:+78695194	None:intergenic
AAATCATAATCATGAGTGGA+GGG	0.582372	2:-78695298	MsG0280011034.01.T01:exon
AACAAATTCCTAACTTTGTC+AGG	0.325137	2:-78695506	MsG0280011034.01.T01:five_prime_UTR
AACAAGACCCTGACAAAGTT+AGG	0.404982	2:+78695498	None:intergenic
AACAATGAAAATAATAACGT+TGG	0.597447	2:+78695121	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAACCTAATAGATGTGTG+AGG	-	Chr2:78693619-78693638	MsG0280011034.01.T01:intron	30.0%
AAAAAGAAGACTCAACATGT+TGG	+	Chr2:78693978-78693997	None:intergenic	30.0%
AAAACCTAATAGATGTGTGA+GGG	-	Chr2:78693620-78693639	MsG0280011034.01.T01:intron	30.0%
AAAATCATAATCATGAGTGG+AGG	-	Chr2:78692679-78692698	MsG0280011034.01.T01:exon	30.0%
AAAATCATCTATAATCACGG+AGG	-	Chr2:78692580-78692599	MsG0280011034.01.T01:three_prime_UTR	30.0%
AAATCATAATCATGAGTGGA+GGG	-	Chr2:78692680-78692699	MsG0280011034.01.T01:CDS	30.0%
AACAAAAACGTGTGTTTGTA+AGG	-	Chr2:78693578-78693597	MsG0280011034.01.T01:intron	30.0%
AACAAATTCCTAACTTTGTC+AGG	-	Chr2:78692472-78692491	MsG0280011034.01.T01:three_prime_UTR	30.0%
AACATGTGTTTATAAATGGG+GGG	-	Chr2:78693440-78693459	MsG0280011034.01.T01:CDS	30.0%
ACAAATTCCTAACTTTGTCA+GGG	-	Chr2:78692473-78692492	MsG0280011034.01.T01:three_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	78692422	78695578	78692422	ID=MsG0280011034.01;Name=MsG0280011034.01
Chr2	mRNA	78692422	78695578	78692422	ID=MsG0280011034.01.T01;Parent=MsG0280011034.01;Name=MsG0280011034.01.T01;_AED=0.39;_eAED=0.39;_QI=269\|0.75\|0.8\|1\|1\|1\|5\|258\|333
Chr2	exon	78692422	78692805	78692422	ID=MsG0280011034.01.T01:exon:21327;Parent=MsG0280011034.01.T01
Chr2	exon	78692941	78693067	78692941	ID=MsG0280011034.01.T01:exon:21326;Parent=MsG0280011034.01.T01
Chr2	exon	78693219	78693481	78693219	ID=MsG0280011034.01.T01:exon:21325;Parent=MsG0280011034.01.T01
Chr2	exon	78693958	78694132	78693958	ID=MsG0280011034.01.T01:exon:21324;Parent=MsG0280011034.01.T01
Chr2	exon	78694999	78695578	78694999	ID=MsG0280011034.01.T01:exon:21323;Parent=MsG0280011034.01.T01
Chr2	five_prime_UTR	78695310	78695578	78695310	ID=MsG0280011034.01.T01:five_prime_utr;Parent=MsG0280011034.01.T01
Chr2	CDS	78694999	78695309	78694999	ID=MsG0280011034.01.T01:cds;Parent=MsG0280011034.01.T01
Chr2	CDS	78693958	78694132	78693958	ID=MsG0280011034.01.T01:cds;Parent=MsG0280011034.01.T01
Chr2	CDS	78693219	78693481	78693219	ID=MsG0280011034.01.T01:cds;Parent=MsG0280011034.01.T01
Chr2	CDS	78692941	78693067	78692941	ID=MsG0280011034.01.T01:cds;Parent=MsG0280011034.01.T01
Chr2	CDS	78692680	78692805	78692680	ID=MsG0280011034.01.T01:cds;Parent=MsG0280011034.01.T01
Chr2	three_prime_UTR	78692422	78692679	78692422	ID=MsG0280011034.01.T01:three_prime_utr;Parent=MsG0280011034.01.T01

Gene Sequence

>MsG0280011034.01.T01

ATGAGTGGAGGGAGGAACCCACATGGTGGCACCACCGTTGAGGTTAAGGTTCATCCTCGCCGTGGGTCTCCGTCTTCGTCGTCGTCGAATCCTCCCGTCACTCGTGATCGTGATTTCAGGCTCTTTAAGCAATGGGTTCCATGGCTTGTTCCAACTTTTGTGTTTGCCAACGTTATTATTTTCATTGTTACAATGTATGTTAATGATTGTCCTGAGAATGCATTTCATGGTACTTGTGTTGCTCCTTTTCTTGGAAGATTCTCCTTTCAACCACTTAAAGAAAACCCTCTCTTTGGTCCTTCTTCCTTTACATTACAGGAGATGGGTGCTCTAGAAGTAGATAAAGTGGTTCATGAACACGAAGGTTGGCGCCTTGTCTCTTGTATATGGTTGCATGGTGGGGCTATCCATGTACTTGCCAACATGTTGAGTCTTCTTTTTATTGGAATTCGGCTTGAACAAGAATTTGGATTTGGTATGGTTCTGATGGCTGCATTGTTGACTCTTATAGTCATTGTTGTAATAAATTTAGCTGTTGGAGTCCTTCCACATGTTGACAATTTCGCTCATCTAGGAGGATTTGGTTCTGGCTTTCTGCTTGGATTTGTCCTTTTGATTCGTCCTCAGTTTAAATGGATAAGCCAACAACAGTCTAAATCTGGATCTCATGCATTTTCTGTTAAACACAATAAGTACTTACCTTATCAATATGTGTTGTGGGTTTTGTCTTTCCTATTACTCATTGCAGGATTCATTACTGGGATGGTTCTACTCTTTCGAGGTGTTAACTTGAATGAACGTTGCTCGTGGTGTCATTATTTAAGTTGTGTTCCTACCTCAAAATGGAGTTGTCAGGAACAAAGCATTTATTGTGAGACAACGGAATTCGGAAATCAACTTAACATCACATGCATGAGCAATGGTAGAAGTGACATTTATCCTCTGTCGAATACTAGCTCTTCTGATAGACAACAACTTTGTTCGCAACTTTGCCATGGATAG

Protein sequence

>MsG0280011034.01.T01

MSGGRNPHGGTTVEVKVHPRRGSPSSSSSNPPVTRDRDFRLFKQWVPWLVPTFVFANVIIFIVTMYVNDCPENAFHGTCVAPFLGRFSFQPLKENPLFGPSSFTLQEMGALEVDKVVHEHEGWRLVSCIWLHGGAIHVLANMLSLLFIGIRLEQEFGFGMVLMAALLTLIVIVVINLAVGVLPHVDNFAHLGGFGSGFLLGFVLLIRPQFKWISQQQSKSGSHAFSVKHNKYLPYQYVLWVLSFLLLIAGFITGMVLLFRGVNLNERCSWCHYLSCVPTSKWSCQEQSIYCETTEFGNQLNITCMSNGRSDIYPLSNTSSSDRQQLCSQLCHG*