Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004143.01.T01 | XP_003590857.2 | 97.015 | 201 | 1 | 2 | 1 | 200 | 1 | 197 | 7.92E-138 | 393 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004143.01.T01 | O82268 | 81.595 | 163 | 20 | 1 | 35 | 187 | 23 | 185 | 4.64E-83 | 248 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004143.01.T01 | G7I5I7 | 97.015 | 201 | 1 | 2 | 1 | 200 | 1 | 197 | 3.78e-138 | 393 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000178.01 | MsG0180004143.01 | 0.810239 | 1.272165e-50 | 7.628471e-48 |
| MsG0180000353.01 | MsG0180004143.01 | 0.803489 | 3.401518e-49 | 1.711621e-46 |
| MsG0180000373.01 | MsG0180004143.01 | 0.809356 | 1.970545e-50 | 1.154494e-47 |
| MsG0180003361.01 | MsG0180004143.01 | 0.870220 | 1.720267e-66 | 6.639706e-63 |
| MsG0180003995.01 | MsG0180004143.01 | 0.801205 | 1.005158e-48 | 4.772054e-46 |
| MsG0180004143.01 | MsG0280007414.01 | 0.846830 | 1.697664e-59 | 2.959202e-56 |
| MsG0180004143.01 | MsG0280010491.01 | 0.806435 | 8.238765e-50 | 4.471375e-47 |
| MsG0180004143.01 | MsG0280010650.01 | 0.805125 | 1.552689e-49 | 8.148874e-47 |
| MsG0180004143.01 | MsG0280011258.01 | 0.836165 | 1.097944e-56 | 1.372440e-53 |
| MsG0180004143.01 | MsG0280011282.01 | 0.820304 | 7.360297e-53 | 5.802242e-50 |
| MsG0180004143.01 | MsG0380013475.01 | 0.801275 | 9.723617e-49 | 4.624611e-46 |
| MsG0180004143.01 | MsG0380017869.01 | 0.809790 | 1.589614e-50 | 9.421045e-48 |
| MsG0180004143.01 | MsG0480018919.01 | 0.810566 | 1.081304e-50 | 6.540279e-48 |
| MsG0180004143.01 | MsG0580024489.01 | 0.810335 | 1.213159e-50 | 7.292708e-48 |
| MsG0180004143.01 | MsG0580025740.01 | 0.810356 | 1.200339e-50 | 7.219868e-48 |
| MsG0180004143.01 | MsG0580026610.01 | 0.826997 | 1.994917e-54 | 1.901256e-51 |
| MsG0180004143.01 | MsG0580028176.01 | 0.801377 | 9.264670e-49 | 4.417722e-46 |
| MsG0180004143.01 | MsG0580029640.01 | 0.816019 | 6.862293e-52 | 4.807214e-49 |
| MsG0180004143.01 | MsG0780036147.01 | 0.815316 | 9.842098e-52 | 6.762838e-49 |
| MsG0180004143.01 | MsG0780036477.01 | 0.809410 | 1.918124e-50 | 1.125422e-47 |
| MsG0180004143.01 | MsG0780038424.01 | 0.826992 | 2.001007e-54 | 1.906738e-51 |
| MsG0180004143.01 | MsG0880041860.01 | 0.809561 | 1.780581e-50 | 1.048873e-47 |
| MsG0180004143.01 | MsG0880042961.01 | 0.850778 | 1.362811e-60 | 2.699319e-57 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004143.01.T01 | MTR_1g075990 | 97.015 | 201 | 1 | 2 | 1 | 200 | 1 | 197 | 9.59e-142 | 393 |
| MsG0180004143.01.T01 | MTR_1g442860 | 71.958 | 189 | 44 | 2 | 1 | 180 | 1 | 189 | 4.99e-91 | 265 |
| MsG0180004143.01.T01 | MTR_4g094428 | 81.119 | 143 | 22 | 1 | 44 | 186 | 39 | 176 | 3.00e-86 | 254 |
| MsG0180004143.01.T01 | MTR_5g072510 | 78.571 | 140 | 30 | 0 | 50 | 189 | 31 | 170 | 4.68e-84 | 248 |
| MsG0180004143.01.T01 | MTR_7g097030 | 93.333 | 135 | 9 | 0 | 52 | 186 | 60 | 194 | 1.04e-82 | 244 |
| MsG0180004143.01.T01 | MTR_1g069825 | 94.656 | 131 | 7 | 0 | 56 | 186 | 52 | 182 | 1.47e-81 | 242 |
| MsG0180004143.01.T01 | MTR_2g016110 | 71.622 | 148 | 42 | 0 | 43 | 190 | 22 | 169 | 1.87e-74 | 223 |
| MsG0180004143.01.T01 | MTR_3g031830 | 80.597 | 134 | 26 | 0 | 56 | 189 | 42 | 175 | 3.36e-74 | 223 |
| MsG0180004143.01.T01 | MTR_4g079930 | 68.707 | 147 | 41 | 1 | 33 | 179 | 11 | 152 | 2.55e-73 | 219 |
| MsG0180004143.01.T01 | MTR_2g092950 | 67.550 | 151 | 43 | 1 | 29 | 179 | 2 | 146 | 2.84e-73 | 219 |
| MsG0180004143.01.T01 | MTR_2g076400 | 75.182 | 137 | 34 | 0 | 54 | 190 | 31 | 167 | 1.06e-72 | 218 |
| MsG0180004143.01.T01 | MTR_7g115700 | 65.217 | 161 | 49 | 3 | 28 | 187 | 4 | 158 | 2.87e-71 | 214 |
| MsG0180004143.01.T01 | MTR_1g080210 | 83.200 | 125 | 21 | 0 | 56 | 180 | 40 | 164 | 1.10e-68 | 207 |
| MsG0180004143.01.T01 | MTR_8g086350 | 73.016 | 126 | 34 | 0 | 54 | 179 | 29 | 154 | 1.80e-57 | 179 |
| MsG0180004143.01.T01 | MTR_1g114020 | 74.510 | 51 | 13 | 0 | 49 | 99 | 64 | 114 | 3.59e-21 | 86.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004143.01.T01 | AT2G31160 | 81.595 | 163 | 20 | 1 | 35 | 187 | 23 | 185 | 4.74e-84 | 248 |
| MsG0180004143.01.T01 | AT1G07090 | 78.723 | 141 | 27 | 1 | 47 | 187 | 25 | 162 | 1.48e-83 | 246 |
| MsG0180004143.01.T01 | AT3G23290 | 78.235 | 170 | 29 | 1 | 19 | 180 | 3 | 172 | 5.28e-82 | 242 |
| MsG0180004143.01.T01 | AT5G58500 | 76.596 | 141 | 32 | 1 | 55 | 194 | 18 | 158 | 4.76e-80 | 236 |
| MsG0180004143.01.T01 | AT5G28490 | 81.379 | 145 | 23 | 1 | 41 | 185 | 14 | 154 | 9.06e-74 | 221 |
| MsG0180004143.01.T01 | AT3G04510 | 82.308 | 130 | 23 | 0 | 56 | 185 | 33 | 162 | 1.08e-70 | 214 |
| MsG0180004143.01.T01 | AT2G42610 | 71.233 | 146 | 39 | 1 | 42 | 187 | 14 | 156 | 1.85e-70 | 212 |
| MsG0180004143.01.T01 | AT2G42610 | 71.233 | 146 | 39 | 1 | 42 | 187 | 14 | 156 | 1.85e-70 | 212 |
| MsG0180004143.01.T01 | AT1G78815 | 70.079 | 127 | 38 | 0 | 53 | 179 | 37 | 163 | 5.70e-66 | 201 |
| MsG0180004143.01.T01 | AT4G18610 | 69.841 | 126 | 37 | 1 | 55 | 179 | 36 | 161 | 5.25e-63 | 194 |
| MsG0180004143.01.T01 | AT1G16910 | 65.909 | 132 | 45 | 0 | 53 | 184 | 20 | 151 | 2.31e-62 | 191 |
Find 35 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTAGAGTGTTTGTCGTTGC+TGG | 0.294103 | 1:+73939933 | None:intergenic |
| AGAAGAGACTGGAACACTTT+TGG | 0.322091 | 1:-73939891 | MsG0180004143.01.T01:CDS |
| TTAAGGTATCTTGATCAGTT+TGG | 0.338884 | 1:-73939798 | MsG0180004143.01.T01:CDS |
| TAAGGTATCTTGATCAGTTT+GGG | 0.342770 | 1:-73939797 | MsG0180004143.01.T01:CDS |
| GACTCTTTCTTCGTGAAGTT+AGG | 0.348358 | 1:-73939593 | MsG0180004143.01.T01:CDS |
| GGACAAGGTGCAGGTGGGTT+TGG | 0.354086 | 1:+73939732 | None:intergenic |
| TTGTCCTCTCAGACAAGCTT+GGG | 0.367576 | 1:-73939709 | MsG0180004143.01.T01:CDS |
| GTCTTCTGTGGTTGTTGTGT+TGG | 0.384530 | 1:+73940004 | None:intergenic |
| AAACCTGAAGATAACCCTTT+TGG | 0.390636 | 1:-73939630 | MsG0180004143.01.T01:CDS |
| GGTGGTGGAGGTTGAATTTG+AGG | 0.396468 | 1:+73939516 | None:intergenic |
| ACTTAATTAGTTGCATTTGA+TGG | 0.399489 | 1:+73939492 | None:intergenic |
| GCACCAAAAGGGTTATCTTC+AGG | 0.403413 | 1:+73939627 | None:intergenic |
| ACTCTTTCTTCGTGAAGTTA+GGG | 0.427767 | 1:-73939592 | MsG0180004143.01.T01:CDS |
| CTTGTCCTCTCAGACAAGCT+TGG | 0.437451 | 1:-73939710 | MsG0180004143.01.T01:CDS |
| GCATTTGATGGTGGTGGTGG+AGG | 0.439596 | 1:+73939504 | None:intergenic |
| GTTGCATTTGATGGTGGTGG+TGG | 0.444483 | 1:+73939501 | None:intergenic |
| TGATCAGTTTGGGAAAACTA+AGG | 0.457631 | 1:-73939787 | MsG0180004143.01.T01:CDS |
| GGAAGTCTTGATGCATTGAT+TGG | 0.461550 | 1:-73939687 | MsG0180004143.01.T01:CDS |
| CTAACAGCACGAGCACCAAA+AGG | 0.492363 | 1:+73939615 | None:intergenic |
| AGAGTGAAAGTGGTGGTCTA+TGG | 0.493036 | 1:+73939853 | None:intergenic |
| TTAGTTGCATTTGATGGTGG+TGG | 0.493298 | 1:+73939498 | None:intergenic |
| CTTTCACTCTCACGTTGTAG+TGG | 0.531680 | 1:-73939840 | MsG0180004143.01.T01:CDS |
| AGAGGACAAGGACAAGGTGC+AGG | 0.565916 | 1:+73939723 | None:intergenic |
| CAAGCTTGTCTGAGAGGACA+AGG | 0.577230 | 1:+73939711 | None:intergenic |
| CTACAACGTGAGAGTGAAAG+TGG | 0.580478 | 1:+73939843 | None:intergenic |
| GGACAAGGACAAGGTGCAGG+TGG | 0.580568 | 1:+73939726 | None:intergenic |
| CTTCCCCAAGCTTGTCTGAG+AGG | 0.586547 | 1:+73939705 | None:intergenic |
| TAACAGCACGAGCACCAAAA+GGG | 0.587993 | 1:+73939616 | None:intergenic |
| GACTCTCAATCTAAAGCTAG+AGG | 0.600860 | 1:-73939570 | MsG0180004143.01.T01:CDS |
| TAATTAGTTGCATTTGATGG+TGG | 0.602526 | 1:+73939495 | None:intergenic |
| AGAGTGTTTGTCGTTGCTGG+TGG | 0.637477 | 1:+73939936 | None:intergenic |
| GACAAGGACAAGGTGCAGGT+GGG | 0.650602 | 1:+73939727 | None:intergenic |
| TGTCCTCTCAGACAAGCTTG+GGG | 0.658773 | 1:-73939708 | MsG0180004143.01.T01:CDS |
| TGTCTGAGAGGACAAGGACA+AGG | 0.664143 | 1:+73939717 | None:intergenic |
| CAACGTGAGAGTGAAAGTGG+TGG | 0.746060 | 1:+73939846 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CACATGTTCTTGAATTTTTA+AGG | - | Chr1:73939753-73939772 | MsG0180004143.01.T01:CDS | 25.0% |
| AGAATCAAAAAAGAAGAGAC+TGG | - | Chr1:73939666-73939685 | MsG0180004143.01.T01:CDS | 30.0% | |
| TAATTAGTTGCATTTGATGG+TGG | + | Chr1:73940076-73940095 | None:intergenic | 30.0% | |
| ! | TAAGGTATCTTGATCAGTTT+GGG | - | Chr1:73939771-73939790 | MsG0180004143.01.T01:CDS | 30.0% |
| ! | TTAAGGTATCTTGATCAGTT+TGG | - | Chr1:73939770-73939789 | MsG0180004143.01.T01:CDS | 30.0% |
| !!! | TCACAGATTTGTCCATTTTT+TGG | - | Chr1:73939809-73939828 | MsG0180004143.01.T01:CDS | 30.0% |
| ACTCTTTCTTCGTGAAGTTA+GGG | - | Chr1:73939976-73939995 | MsG0180004143.01.T01:CDS | 35.0% | |
| TGATCAGTTTGGGAAAACTA+AGG | - | Chr1:73939781-73939800 | MsG0180004143.01.T01:CDS | 35.0% | |
| ! | AAACCTGAAGATAACCCTTT+TGG | - | Chr1:73939938-73939957 | MsG0180004143.01.T01:CDS | 35.0% |
| !! | GATGTTTTTGTTGTCTTCTG+TGG | + | Chr1:73939579-73939598 | None:intergenic | 35.0% |
| GACTCTTTCTTCGTGAAGTT+AGG | - | Chr1:73939975-73939994 | MsG0180004143.01.T01:CDS | 40.0% | |
| GGGTTTGGATGACCAAAAAA+TGG | + | Chr1:73939824-73939843 | None:intergenic | 40.0% | |
| ! | AGAAGAGACTGGAACACTTT+TGG | - | Chr1:73939677-73939696 | MsG0180004143.01.T01:CDS | 40.0% |
| ! | GACTCTCAATCTAAAGCTAG+AGG | - | Chr1:73939998-73940017 | MsG0180004143.01.T01:CDS | 40.0% |
| ! | GGAAGTCTTGATGCATTGAT+TGG | - | Chr1:73939881-73939900 | MsG0180004143.01.T01:CDS | 40.0% |
| !! | CTGCTTTTGAAGAGAATGGT+GGG | - | Chr1:73939915-73939934 | MsG0180004143.01.T01:CDS | 40.0% |
| !! | TTAGTTGCATTTGATGGTGG+TGG | + | Chr1:73940073-73940092 | None:intergenic | 40.0% |
| !!! | AGAGCTGCTTTTGAAGAGAA+TGG | - | Chr1:73939911-73939930 | MsG0180004143.01.T01:CDS | 40.0% |
| AGAGTGAAAGTGGTGGTCTA+TGG | + | Chr1:73939718-73939737 | None:intergenic | 45.0% | |
| CTACAACGTGAGAGTGAAAG+TGG | + | Chr1:73939728-73939747 | None:intergenic | 45.0% | |
| CTTTCACTCTCACGTTGTAG+TGG | - | Chr1:73939728-73939747 | MsG0180004143.01.T01:CDS | 45.0% | |
| GCACCAAAAGGGTTATCTTC+AGG | + | Chr1:73939944-73939963 | None:intergenic | 45.0% | |
| GTCTTCTGTGGTTGTTGTGT+TGG | + | Chr1:73939567-73939586 | None:intergenic | 45.0% | |
| TAACAGCACGAGCACCAAAA+GGG | + | Chr1:73939955-73939974 | None:intergenic | 45.0% | |
| TTGTCCTCTCAGACAAGCTT+GGG | - | Chr1:73939859-73939878 | MsG0180004143.01.T01:CDS | 45.0% | |
| ! | CTTAGAGTGTTTGTCGTTGC+TGG | + | Chr1:73939638-73939657 | None:intergenic | 45.0% |
| !!! | GCTGCTTTTGAAGAGAATGG+TGG | - | Chr1:73939914-73939933 | MsG0180004143.01.T01:CDS | 45.0% |
| CAACGTGAGAGTGAAAGTGG+TGG | + | Chr1:73939725-73939744 | None:intergenic | 50.0% | |
| CAAGCTTGTCTGAGAGGACA+AGG | + | Chr1:73939860-73939879 | None:intergenic | 50.0% | |
| CTAACAGCACGAGCACCAAA+AGG | + | Chr1:73939956-73939975 | None:intergenic | 50.0% | |
| CTTGTCCTCTCAGACAAGCT+TGG | - | Chr1:73939858-73939877 | MsG0180004143.01.T01:CDS | 50.0% | |
| TGTCCTCTCAGACAAGCTTG+GGG | - | Chr1:73939860-73939879 | MsG0180004143.01.T01:CDS | 50.0% | |
| TGTCTGAGAGGACAAGGACA+AGG | + | Chr1:73939854-73939873 | None:intergenic | 50.0% | |
| ! | AGAGTGTTTGTCGTTGCTGG+TGG | + | Chr1:73939635-73939654 | None:intergenic | 50.0% |
| !! | GGTGGTGGAGGTTGAATTTG+AGG | + | Chr1:73940055-73940074 | None:intergenic | 50.0% |
| !! | GTTGCATTTGATGGTGGTGG+TGG | + | Chr1:73940070-73940089 | None:intergenic | 50.0% |
| AGAGGACAAGGACAAGGTGC+AGG | + | Chr1:73939848-73939867 | None:intergenic | 55.0% | |
| CTTCCCCAAGCTTGTCTGAG+AGG | + | Chr1:73939866-73939885 | None:intergenic | 55.0% | |
| GACAAGGACAAGGTGCAGGT+GGG | + | Chr1:73939844-73939863 | None:intergenic | 55.0% | |
| !! | GCATTTGATGGTGGTGGTGG+AGG | + | Chr1:73940067-73940086 | None:intergenic | 55.0% |
| GGACAAGGACAAGGTGCAGG+TGG | + | Chr1:73939845-73939864 | None:intergenic | 60.0% | |
| ! | GGACAAGGTGCAGGTGGGTT+TGG | + | Chr1:73939839-73939858 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 73939494 | 73940096 | 73939494 | ID=MsG0180004143.01;Name=MsG0180004143.01 |
| Chr1 | mRNA | 73939494 | 73940096 | 73939494 | ID=MsG0180004143.01.T01;Parent=MsG0180004143.01;Name=MsG0180004143.01.T01;_AED=0.36;_eAED=0.36;_QI=0|-1|0|1|-1|1|1|0|200 |
| Chr1 | exon | 73939494 | 73940096 | 73939494 | ID=MsG0180004143.01.T01:exon:5419;Parent=MsG0180004143.01.T01 |
| Chr1 | CDS | 73939494 | 73940096 | 73939494 | ID=MsG0180004143.01.T01:cds;Parent=MsG0180004143.01.T01 |
| Gene Sequence |
| Protein sequence |
