Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004947.01.T01 | GAU16916.1 | 51.948 | 77 | 36 | 1 | 1 | 76 | 38 | 114 | 2.64E-15 | 75.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004947.01.T01 | Q1RSH2 | 51.948 | 77 | 36 | 1 | 1 | 76 | 142 | 218 | 1.01e-14 | 75.5 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004947.01 | MsG0180006047.01 | 0.846291 | 2.382256e-59 | 4.080565e-56 |
| MsG0180004947.01 | MsG0280007272.01 | 0.818317 | 2.087882e-52 | 1.557628e-49 |
| MsG0180004947.01 | MsG0280007369.01 | 0.806788 | 6.938064e-50 | 3.799698e-47 |
| MsG0180004947.01 | MsG0280007391.01 | 0.804881 | 1.746107e-49 | 9.107338e-47 |
| MsG0180004947.01 | MsG0280011053.01 | 0.829003 | 6.563232e-55 | 6.631352e-52 |
| MsG0180004947.01 | MsG0580028416.01 | 0.848542 | 5.736463e-60 | 1.056276e-56 |
| MsG0180004947.01 | MsG0580029758.01 | 0.806372 | 8.494136e-50 | 4.602586e-47 |
| MsG0180004947.01 | MsG0680031802.01 | 0.809537 | 1.802026e-50 | 1.060821e-47 |
| MsG0180004947.01 | MsG0680033951.01 | 0.809161 | 2.169547e-50 | 1.264460e-47 |
| MsG0180004947.01 | MsG0780036514.01 | 0.819398 | 1.186102e-52 | 9.116971e-50 |
| MsG0180004947.01 | MsG0780036854.01 | 0.802505 | 5.434789e-49 | 2.666772e-46 |
| MsG0180004947.01 | MsG0780038925.01 | 0.848972 | 4.358602e-60 | 8.138902e-57 |
| MsG0180004947.01 | MsG0780038930.01 | 0.823707 | 1.197985e-53 | 1.039566e-50 |
| MsG0180004947.01 | MsG0880042843.01 | 0.804875 | 1.751062e-49 | 9.131880e-47 |
| MsG0180004947.01 | MsG0880047375.01 | 0.802091 | 6.612357e-49 | 3.210578e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004947.01.T01 | MTR_1g090150 | 51.948 | 77 | 36 | 1 | 1 | 76 | 142 | 218 | 2.57e-18 | 75.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCATTTCCCCACCTTCACTC+TGG | 0.346021 | 1:+84769719 | MsG0180004947.01.T01:CDS |
| TTCATTGGTGGCAACACAAT+TGG | 0.349905 | 1:+84769612 | MsG0180004947.01.T01:CDS |
| ACTCTGGGTCAAGAATTTCT+TGG | 0.351083 | 1:+84769735 | MsG0180004947.01.T01:CDS |
| CATTTCCCCACCTTCACTCT+GGG | 0.421521 | 1:+84769720 | MsG0180004947.01.T01:CDS |
| GCTACTTGTTGAGTACTTCA+AGG | 0.437991 | 1:+84769793 | MsG0180004947.01.T01:CDS |
| GGATTGGCAGACATAGCCTT+AGG | 0.466349 | 1:+84769633 | MsG0180004947.01.T01:CDS |
| CCAGTCATGGAAGAAATTGT+TGG | 0.492761 | 1:+84769675 | MsG0180004947.01.T01:CDS |
| TGGCAGACATAGCCTTAGGT+TGG | 0.497374 | 1:+84769637 | MsG0180004947.01.T01:CDS |
| CCAACAATTTCTTCCATGAC+TGG | 0.525633 | 1:-84769675 | None:intergenic |
| TTCTTGACCCAGAGTGAAGG+TGG | 0.531542 | 1:-84769727 | None:intergenic |
| GTACTCAACAAGTAGCTCAT+GGG | 0.541505 | 1:-84769786 | None:intergenic |
| TGGTGGCAACACAATTGGAT+TGG | 0.546011 | 1:+84769617 | MsG0180004947.01.T01:CDS |
| TGCACATACTCTGCCAGTCA+TGG | 0.554228 | 1:+84769662 | MsG0180004947.01.T01:CDS |
| GGTAGTGAAAAGATGTTCAT+TGG | 0.554234 | 1:+84769597 | None:intergenic |
| AGTACTCAACAAGTAGCTCA+TGG | 0.570685 | 1:-84769787 | None:intergenic |
| AGTATGTGCAATCCAACCTA+AGG | 0.590838 | 1:-84769649 | None:intergenic |
| TTGAGTACTTCAAGGAAAGG+AGG | 0.598211 | 1:+84769801 | MsG0180004947.01.T01:CDS |
| TTGTTGAGTACTTCAAGGAA+AGG | 0.601650 | 1:+84769798 | MsG0180004947.01.T01:CDS |
| AGTGAAAAGATGTTCATTGG+TGG | 0.611042 | 1:+84769600 | None:intergenic |
| CTTGACCCAGAGTGAAGGTG+GGG | 0.613279 | 1:-84769725 | None:intergenic |
| AAATTCTTGACCCAGAGTGA+AGG | 0.613532 | 1:-84769730 | None:intergenic |
| TCTTGACCCAGAGTGAAGGT+GGG | 0.622407 | 1:-84769726 | None:intergenic |
| TACTCAACAAGTAGCTCATG+GGG | 0.634231 | 1:-84769785 | None:intergenic |
| GTACTTCAAGGAAAGGAGGA+AGG | 0.655832 | 1:+84769805 | MsG0180004947.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTGAGATTGTTTTTTATAGC+AGG | - | Chr1:84769766-84769785 | None:intergenic | 30.0% |
| TTGTTGAGTACTTCAAGGAA+AGG | + | Chr1:84769798-84769817 | MsG0180004947.01.T01:CDS | 35.0% | |
| AAATTCTTGACCCAGAGTGA+AGG | - | Chr1:84769733-84769752 | None:intergenic | 40.0% | |
| ACTCTGGGTCAAGAATTTCT+TGG | + | Chr1:84769735-84769754 | MsG0180004947.01.T01:CDS | 40.0% | |
| AGTACTCAACAAGTAGCTCA+TGG | - | Chr1:84769790-84769809 | None:intergenic | 40.0% | |
| AGTATGTGCAATCCAACCTA+AGG | - | Chr1:84769652-84769671 | None:intergenic | 40.0% | |
| CCAACAATTTCTTCCATGAC+TGG | - | Chr1:84769678-84769697 | None:intergenic | 40.0% | |
| CCAGTCATGGAAGAAATTGT+TGG | + | Chr1:84769675-84769694 | MsG0180004947.01.T01:CDS | 40.0% | |
| GTACTCAACAAGTAGCTCAT+GGG | - | Chr1:84769789-84769808 | None:intergenic | 40.0% | |
| TACTCAACAAGTAGCTCATG+GGG | - | Chr1:84769788-84769807 | None:intergenic | 40.0% | |
| TTGAGTACTTCAAGGAAAGG+AGG | + | Chr1:84769801-84769820 | MsG0180004947.01.T01:CDS | 40.0% | |
| ! | GCTACTTGTTGAGTACTTCA+AGG | + | Chr1:84769793-84769812 | MsG0180004947.01.T01:CDS | 40.0% |
| ! | TTCATTGGTGGCAACACAAT+TGG | + | Chr1:84769612-84769631 | MsG0180004947.01.T01:CDS | 40.0% |
| GTACTTCAAGGAAAGGAGGA+AGG | + | Chr1:84769805-84769824 | MsG0180004947.01.T01:CDS | 45.0% | |
| TGGTGGCAACACAATTGGAT+TGG | + | Chr1:84769617-84769636 | MsG0180004947.01.T01:CDS | 45.0% | |
| CATTTCCCCACCTTCACTCT+GGG | + | Chr1:84769720-84769739 | MsG0180004947.01.T01:CDS | 50.0% | |
| TCTTGACCCAGAGTGAAGGT+GGG | - | Chr1:84769729-84769748 | None:intergenic | 50.0% | |
| TGCACATACTCTGCCAGTCA+TGG | + | Chr1:84769662-84769681 | MsG0180004947.01.T01:CDS | 50.0% | |
| TTCTTGACCCAGAGTGAAGG+TGG | - | Chr1:84769730-84769749 | None:intergenic | 50.0% | |
| ! | GGATTGGCAGACATAGCCTT+AGG | + | Chr1:84769633-84769652 | MsG0180004947.01.T01:CDS | 50.0% |
| ! | TGGCAGACATAGCCTTAGGT+TGG | + | Chr1:84769637-84769656 | MsG0180004947.01.T01:CDS | 50.0% |
| CTTGACCCAGAGTGAAGGTG+GGG | - | Chr1:84769728-84769747 | None:intergenic | 55.0% | |
| GCATTTCCCCACCTTCACTC+TGG | + | Chr1:84769719-84769738 | MsG0180004947.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 84769609 | 84769842 | 84769609 | ID=MsG0180004947.01;Name=MsG0180004947.01 |
| Chr1 | mRNA | 84769609 | 84769842 | 84769609 | ID=MsG0180004947.01.T01;Parent=MsG0180004947.01;Name=MsG0180004947.01.T01;_AED=0.33;_eAED=0.33;_QI=0|-1|0|1|-1|0|1|0|77 |
| Chr1 | exon | 84769609 | 84769842 | 84769609 | ID=MsG0180004947.01.T01:exon:9389;Parent=MsG0180004947.01.T01 |
| Chr1 | CDS | 84769609 | 84769842 | 84769609 | ID=MsG0180004947.01.T01:cds;Parent=MsG0180004947.01.T01 |
| Gene Sequence |
| Protein sequence |
