Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005001.01.T01 | XP_013469311.1 | 88.889 | 135 | 13 | 1 | 1 | 133 | 1 | 135 | 2.32E-76 | 233 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005001.01.T01 | B7FH61 | 88.889 | 135 | 13 | 1 | 1 | 133 | 1 | 135 | 1.11e-76 | 233 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000240.01 | MsG0180005001.01 | 0.819394 | 1.188613e-52 | 9.134965e-50 |
| MsG0180003767.01 | MsG0180005001.01 | 0.815646 | 8.310748e-52 | 5.762897e-49 |
| MsG0180005001.01 | MsG0180006064.01 | 0.837075 | 6.435961e-57 | 8.267267e-54 |
| MsG0180005001.01 | MsG0280006711.01 | 0.843839 | 1.094844e-58 | 1.734723e-55 |
| MsG0180005001.01 | MsG0280010146.01 | 0.825039 | 5.825686e-54 | 5.250338e-51 |
| MsG0180005001.01 | MsG0280010532.01 | 0.813651 | 2.298122e-51 | 1.509477e-48 |
| MsG0180005001.01 | MsG0280010604.01 | 0.802887 | 4.531523e-49 | 2.245340e-46 |
| MsG0180005001.01 | MsG0280011059.01 | 0.826413 | 2.750497e-54 | 2.577714e-51 |
| MsG0180005001.01 | MsG0380016191.01 | 0.833543 | 5.021908e-56 | 5.801715e-53 |
| MsG0180005001.01 | MsG0380016347.01 | 0.818510 | 1.888111e-52 | 1.416129e-49 |
| MsG0180005001.01 | MsG0480023400.01 | 0.801628 | 8.231723e-49 | 3.949926e-46 |
| MsG0180005001.01 | MsG0480023936.01 | 0.803405 | 3.541189e-49 | 1.778026e-46 |
| MsG0180005001.01 | MsG0580024790.01 | 0.821071 | 4.905001e-53 | 3.950384e-50 |
| MsG0180005001.01 | MsG0580025369.01 | 0.805870 | 1.083417e-49 | 5.795974e-47 |
| MsG0180005001.01 | MsG0680031805.01 | 0.806036 | 9.996247e-50 | 5.370441e-47 |
| MsG0180005001.01 | MsG0880045010.01 | 0.807790 | 4.254424e-50 | 2.391501e-47 |
| MsG0180005001.01 | MsG0880046289.01 | 0.817355 | 3.442915e-52 | 2.501535e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005001.01.T01 | MTR_1g090837 | 88.889 | 135 | 13 | 1 | 1 | 133 | 1 | 135 | 2.81e-80 | 233 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACGGCGGAGATTCGCAGTTT+TGG | 0.368913 | 1:+85489352 | None:intergenic |
| TTCTTGTAAGAGGAAGCTTA+TGG | 0.390438 | 1:-85489095 | MsG0180005001.01.T01:CDS |
| AGCCGTTTGTTTCTTGTAAG+AGG | 0.398994 | 1:-85489105 | MsG0180005001.01.T01:CDS |
| GACATCGTGTCGGCAACGTC+AGG | 0.417129 | 1:+85489280 | None:intergenic |
| AAGCAAGATACCGGAGATAC+AGG | 0.441445 | 1:-85489155 | MsG0180005001.01.T01:CDS |
| TTACAAGAAACAAACGGCTT+CGG | 0.447163 | 1:+85489109 | None:intergenic |
| TTTCTTTCGACGTCGGAGTC+CGG | 0.456673 | 1:+85489176 | None:intergenic |
| GTGAAATCGATGAGAGTTAC+CGG | 0.456812 | 1:-85489195 | MsG0180005001.01.T01:CDS |
| ACAAGTTCTCTTCGGAAAAG+TGG | 0.496245 | 1:+85489015 | None:intergenic |
| GTACAAGGACAAGTTCTCTT+CGG | 0.515314 | 1:+85489007 | None:intergenic |
| GGCGGACAATCCTGTATCTC+CGG | 0.535721 | 1:+85489145 | None:intergenic |
| TTCCTCTTACAAGAAACAAA+CGG | 0.536839 | 1:+85489103 | None:intergenic |
| ATCTTGCTTTCTTTCGACGT+CGG | 0.541967 | 1:+85489169 | None:intergenic |
| GTCGAAAGAAAGCAAGATAC+CGG | 0.550012 | 1:-85489164 | MsG0180005001.01.T01:CDS |
| ATTGAGAAAGAAAACGTTGA+CGG | 0.551569 | 1:-85489232 | MsG0180005001.01.T01:CDS |
| GTTGACGGTGCTGTTGTTGA+CGG | 0.553828 | 1:-85489217 | MsG0180005001.01.T01:CDS |
| GTCGTTCTCGATGACGTCGA+CGG | 0.585755 | 1:+85489333 | None:intergenic |
| TCAGGGACTTGATCGGAGGA+AGG | 0.617553 | 1:+85489298 | None:intergenic |
| TAATTGCTGGAGGTGAAGAT+CGG | 0.620642 | 1:+85489375 | None:intergenic |
| TGCCGACACGATGTCGTCGT+CGG | 0.622733 | 1:-85489272 | MsG0180005001.01.T01:CDS |
| GGCAACGTCAGGGACTTGAT+CGG | 0.627390 | 1:+85489291 | None:intergenic |
| ACATCGTGTCGGCAACGTCA+GGG | 0.631276 | 1:+85489281 | None:intergenic |
| TGCCGACGACGACATCGTGT+CGG | 0.644546 | 1:+85489270 | None:intergenic |
| GGAGATACAGGATTGTCCGC+CGG | 0.647121 | 1:-85489143 | MsG0180005001.01.T01:CDS |
| GAGGTGAAGATCGGTAGACA+TGG | 0.661979 | 1:+85489384 | None:intergenic |
| AACGTCAGGGACTTGATCGG+AGG | 0.670622 | 1:+85489294 | None:intergenic |
| GTTCTCGATGACGTCGACGG+CGG | 0.690884 | 1:+85489336 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATTGAGAAAGAAAACGTTGA+CGG | - | Chr1:85489153-85489172 | MsG0180005001.01.T01:CDS | 30.0% | |
| TTCCTCTTACAAGAAACAAA+CGG | + | Chr1:85489285-85489304 | None:intergenic | 30.0% | |
| TTACAAGAAACAAACGGCTT+CGG | + | Chr1:85489279-85489298 | None:intergenic | 35.0% | |
| TTCTTGTAAGAGGAAGCTTA+TGG | - | Chr1:85489290-85489309 | MsG0180005001.01.T01:CDS | 35.0% | |
| ACAAGTTCTCTTCGGAAAAG+TGG | + | Chr1:85489373-85489392 | None:intergenic | 40.0% | |
| ATCTTGCTTTCTTTCGACGT+CGG | + | Chr1:85489219-85489238 | None:intergenic | 40.0% | |
| GTACAAGGACAAGTTCTCTT+CGG | + | Chr1:85489381-85489400 | None:intergenic | 40.0% | |
| GTCGAAAGAAAGCAAGATAC+CGG | - | Chr1:85489221-85489240 | MsG0180005001.01.T01:CDS | 40.0% | |
| GTGAAATCGATGAGAGTTAC+CGG | - | Chr1:85489190-85489209 | MsG0180005001.01.T01:CDS | 40.0% | |
| TAATTGCTGGAGGTGAAGAT+CGG | + | Chr1:85489013-85489032 | None:intergenic | 40.0% | |
| ! | AGCCGTTTGTTTCTTGTAAG+AGG | - | Chr1:85489280-85489299 | MsG0180005001.01.T01:CDS | 40.0% |
| !! | TTCGCAGTTTTGGTAATTGC+TGG | + | Chr1:85489026-85489045 | None:intergenic | 40.0% |
| AAGCAAGATACCGGAGATAC+AGG | - | Chr1:85489230-85489249 | MsG0180005001.01.T01:CDS | 45.0% | |
| ! | ACAAACGGCTTCGGTTTTCT+CGG | + | Chr1:85489270-85489289 | None:intergenic | 45.0% |
| !! | GCAGTTTTGGTAATTGCTGG+AGG | + | Chr1:85489023-85489042 | None:intergenic | 45.0% |
| TTTCTTTCGACGTCGGAGTC+CGG | + | Chr1:85489212-85489231 | None:intergenic | 50.0% | |
| !! | GTTGACGGTGCTGTTGTTGA+CGG | - | Chr1:85489168-85489187 | MsG0180005001.01.T01:CDS | 50.0% |
| AACGTCAGGGACTTGATCGG+AGG | + | Chr1:85489094-85489113 | None:intergenic | 55.0% | |
| GGAGATACAGGATTGTCCGC+CGG | - | Chr1:85489242-85489261 | MsG0180005001.01.T01:CDS | 55.0% | |
| GGCAACGTCAGGGACTTGAT+CGG | + | Chr1:85489097-85489116 | None:intergenic | 55.0% | |
| GGCGGACAATCCTGTATCTC+CGG | + | Chr1:85489243-85489262 | None:intergenic | 55.0% | |
| GTCGTTCTCGATGACGTCGA+CGG | + | Chr1:85489055-85489074 | None:intergenic | 55.0% | |
| TCAGGGACTTGATCGGAGGA+AGG | + | Chr1:85489090-85489109 | None:intergenic | 55.0% | |
| ! | ACATCGTGTCGGCAACGTCA+GGG | + | Chr1:85489107-85489126 | None:intergenic | 55.0% |
| ! | ACGGCGGAGATTCGCAGTTT+TGG | + | Chr1:85489036-85489055 | None:intergenic | 55.0% |
| GTTCTCGATGACGTCGACGG+CGG | + | Chr1:85489052-85489071 | None:intergenic | 60.0% | |
| TGCCGACACGATGTCGTCGT+CGG | - | Chr1:85489113-85489132 | MsG0180005001.01.T01:CDS | 60.0% | |
| TGCCGACGACGACATCGTGT+CGG | + | Chr1:85489118-85489137 | None:intergenic | 60.0% | |
| ! | GACATCGTGTCGGCAACGTC+AGG | + | Chr1:85489108-85489127 | None:intergenic | 60.0% |
| ! | GGCTTCGGTTTTCTCGGCGC+CGG | + | Chr1:85489264-85489283 | None:intergenic | 65.0% |
| !! | TTCGGTTTTCTCGGCGCCGG+CGG | + | Chr1:85489261-85489280 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 85489003 | 85489404 | 85489003 | ID=MsG0180005001.01;Name=MsG0180005001.01 |
| Chr1 | mRNA | 85489003 | 85489404 | 85489003 | ID=MsG0180005001.01.T01;Parent=MsG0180005001.01;Name=MsG0180005001.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|133 |
| Chr1 | exon | 85489003 | 85489404 | 85489003 | ID=MsG0180005001.01.T01:exon:14041;Parent=MsG0180005001.01.T01 |
| Chr1 | CDS | 85489003 | 85489404 | 85489003 | ID=MsG0180005001.01.T01:cds;Parent=MsG0180005001.01.T01 |
| Gene Sequence |
| Protein sequence |
