Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180006174.01.T01 | RHN82725.1 | 95.122 | 246 | 10 | 1 | 1 | 246 | 1 | 244 | 2.73E-160 | 454 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180006174.01.T01 | Q9LQQ0 | 34.5 | 200 | 100 | 6 | 49 | 219 | 160 | 357 | 2.32E-23 | 100 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180006174.01.T01 | A0A396K548 | 95.122 | 246 | 10 | 1 | 1 | 246 | 1 | 244 | 1.31e-160 | 454 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080049023.01 | MsG0180006174.01 | -0.817613 | 3.012064e-52 | 2.204027e-49 |
| MsG0180003320.01 | MsG0180006174.01 | 0.809944 | 1.472936e-50 | 8.764566e-48 |
| MsG0180006174.01 | MsG0280007653.01 | -0.803630 | 3.180746e-49 | 1.606286e-46 |
| MsG0180006174.01 | MsG0380014735.01 | -0.804251 | 2.362594e-49 | 1.212257e-46 |
| MsG0180006174.01 | MsG0480020904.01 | 0.822525 | 2.261086e-53 | 1.897314e-50 |
| MsG0180006174.01 | MsG0480021433.01 | 0.802072 | 6.674247e-49 | 3.239016e-46 |
| MsG0180006174.01 | MsG0480022298.01 | 0.815221 | 1.032882e-51 | 7.078593e-49 |
| MsG0180006174.01 | MsG0480022428.01 | 0.810644 | 1.040563e-50 | 6.306914e-48 |
| MsG0180006174.01 | MsG0480022886.01 | 0.800219 | 1.596917e-48 | 7.394529e-46 |
| MsG0180006174.01 | MsG0480023322.01 | 0.810069 | 1.384711e-50 | 8.266474e-48 |
| MsG0180006174.01 | MsG0780038307.01 | 0.801865 | 7.358510e-49 | 3.552485e-46 |
| MsG0180006174.01 | MsG0780039916.01 | 0.804787 | 1.827019e-49 | 9.505601e-47 |
| MsG0180006174.01 | MsG0880043550.01 | 0.832418 | 9.559420e-56 | 1.067879e-52 |
| MsG0180006174.01 | MsG0880044317.01 | -0.802082 | 6.640219e-49 | 3.223370e-46 |
| MsG0180006174.01 | MsG0880045852.01 | 0.803772 | 2.971645e-49 | 1.506199e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180006174.01.T01 | MTR_1g115515 | 94.958 | 238 | 10 | 1 | 9 | 246 | 1 | 236 | 1.66e-157 | 436 |
| MsG0180006174.01.T01 | MTR_1g115510 | 35.593 | 236 | 106 | 10 | 51 | 246 | 123 | 352 | 1.81e-22 | 94.7 |
| MsG0180006174.01.T01 | MTR_1g046800 | 32.057 | 209 | 110 | 5 | 51 | 234 | 39 | 240 | 3.81e-22 | 92.0 |
| MsG0180006174.01.T01 | MTR_7g021300 | 41.538 | 130 | 70 | 2 | 116 | 241 | 259 | 386 | 6.51e-22 | 93.6 |
| MsG0180006174.01.T01 | MTR_4g062470 | 47.573 | 103 | 51 | 3 | 118 | 219 | 178 | 278 | 1.19e-21 | 91.7 |
| MsG0180006174.01.T01 | MTR_4g062520 | 47.619 | 105 | 48 | 4 | 118 | 219 | 177 | 277 | 1.73e-21 | 91.3 |
| MsG0180006174.01.T01 | MTR_1g046500 | 32.536 | 209 | 109 | 6 | 51 | 234 | 39 | 240 | 3.66e-21 | 89.4 |
| MsG0180006174.01.T01 | MTR_4g062460 | 32.000 | 200 | 106 | 6 | 51 | 222 | 38 | 235 | 2.20e-18 | 82.0 |
| MsG0180006174.01.T01 | MTR_1g046490 | 28.910 | 211 | 116 | 4 | 51 | 234 | 23 | 226 | 2.92e-18 | 81.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180006174.01.T01 | AT2G28670 | 50.943 | 106 | 50 | 2 | 114 | 219 | 91 | 194 | 2.36e-24 | 97.4 |
| MsG0180006174.01.T01 | AT1G07730 | 34.500 | 200 | 100 | 6 | 49 | 219 | 160 | 357 | 2.37e-24 | 100 |
| MsG0180006174.01.T01 | AT2G28670 | 34.653 | 202 | 99 | 5 | 49 | 219 | 205 | 404 | 1.62e-23 | 98.6 |
| MsG0180006174.01.T01 | AT4G13580 | 32.338 | 201 | 106 | 5 | 55 | 232 | 39 | 232 | 1.17e-22 | 93.2 |
| MsG0180006174.01.T01 | AT3G24020 | 32.394 | 213 | 114 | 6 | 55 | 244 | 38 | 243 | 3.76e-22 | 92.0 |
| MsG0180006174.01.T01 | AT2G39430 | 31.513 | 238 | 115 | 9 | 51 | 244 | 87 | 320 | 2.52e-21 | 91.3 |
| MsG0180006174.01.T01 | AT3G55230 | 29.832 | 238 | 121 | 8 | 51 | 245 | 70 | 304 | 6.45e-20 | 87.0 |
Find 54 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCATTTCAAGAGCTGGAATT+TGG | 0.209367 | 1:+101000066 | MsG0180006174.01.T01:CDS |
| GGTTTCTCTGTATTAATATT+AGG | 0.221299 | 1:-100999898 | None:intergenic |
| AGCTGAGGAAAATTTGTTTA+TGG | 0.235026 | 1:-100999782 | None:intergenic |
| ATTGTTGCATTGGTTCTCTT+TGG | 0.277906 | 1:-100999706 | None:intergenic |
| GATGGCCATGGTAGTGATTA+GGG | 0.312185 | 1:-100999825 | None:intergenic |
| GTTTGAGTTGATTGTGGAAT+TGG | 0.329326 | 1:-100999994 | None:intergenic |
| TGATGGCCATGGTAGTGATT+AGG | 0.343261 | 1:-100999826 | None:intergenic |
| GAGTCACATGTTGCAGTTAT+TGG | 0.351309 | 1:+101000291 | MsG0180006174.01.T01:CDS |
| CTTCTCTTCTAGCAATTGTC+AGG | 0.383847 | 1:+100999740 | MsG0180006174.01.T01:CDS |
| GGAATTGGAATCCGTCCATT+TGG | 0.401351 | 1:-100999979 | None:intergenic |
| GTTAAGGTTGTGGATAAAGT+AGG | 0.408640 | 1:+101000354 | MsG0180006174.01.T01:CDS |
| GTAGGGTCTAGTACAGTAGA+AGG | 0.416844 | 1:+101000372 | MsG0180006174.01.T01:CDS |
| GGAAAGTACCATGGTGCTAA+TGG | 0.420735 | 1:+101000321 | MsG0180006174.01.T01:CDS |
| TCTTGAAATGCTGCTCTAGT+AGG | 0.424130 | 1:-101000054 | None:intergenic |
| TAATGGCTATGCTGTTGTTA+AGG | 0.440993 | 1:+101000338 | MsG0180006174.01.T01:CDS |
| ACACTTGATCTTTCCACCAT+TGG | 0.449523 | 1:+101000021 | MsG0180006174.01.T01:CDS |
| GTTGCAGTTATTGGTGGTAC+TGG | 0.455685 | 1:+101000300 | MsG0180006174.01.T01:CDS |
| TGCTAGAAGAGAAGGGAGAT+TGG | 0.464219 | 1:-100999731 | None:intergenic |
| GAGATTGGCCATTGTTGCAT+TGG | 0.466148 | 1:-100999716 | None:intergenic |
| TTGAAGGGTGAGTATGAAGA+CGG | 0.484606 | 1:+101000231 | MsG0180006174.01.T01:CDS |
| CTATGCTGTTGTTAAGGTTG+TGG | 0.492544 | 1:+101000344 | MsG0180006174.01.T01:CDS |
| AGAGAAACCTTCCACTACTA+AGG | 0.495734 | 1:+100999912 | MsG0180006174.01.T01:CDS |
| CTGACAATTGCTAGAAGAGA+AGG | 0.504357 | 1:-100999739 | None:intergenic |
| TAGAGCAGCATTTCAAGAGC+TGG | 0.504564 | 1:+101000059 | MsG0180006174.01.T01:CDS |
| GACGAGGAACTACTAGTTGA+AGG | 0.506266 | 1:+101000105 | MsG0180006174.01.T01:CDS |
| TATGTAGCTAGTGAAGAGGA+TGG | 0.509172 | 1:+101000174 | MsG0180006174.01.T01:CDS |
| GTTTGTGTTTGAGTTGATTG+TGG | 0.515184 | 1:-101000000 | None:intergenic |
| TGAAGGGTGAGTATGAAGAC+GGG | 0.516269 | 1:+101000232 | MsG0180006174.01.T01:CDS |
| TTAAGGTTGTGGATAAAGTA+GGG | 0.516469 | 1:+101000355 | MsG0180006174.01.T01:CDS |
| TCACTGTCATAGCAACCATA+TGG | 0.517871 | 1:-101000203 | None:intergenic |
| GAAGATGAACTAAAGAAACT+TGG | 0.528902 | 1:+101000135 | MsG0180006174.01.T01:CDS |
| TCTGTATTAATATTAGGACT+AGG | 0.530173 | 1:-100999892 | None:intergenic |
| ATTGGAATCCGTCCATTTGG+AGG | 0.538678 | 1:-100999976 | None:intergenic |
| CCATGGTAGTGATTAGGGGT+TGG | 0.550854 | 1:-100999820 | None:intergenic |
| AAGAAACTTGGAAAAGCAGA+AGG | 0.552239 | 1:+101000147 | MsG0180006174.01.T01:CDS |
| AGAGGATGGAAGTAGCCATA+TGG | 0.562281 | 1:+101000188 | MsG0180006174.01.T01:CDS |
| TTTGTGACCTTAGTAGTGGA+AGG | 0.562764 | 1:-100999919 | None:intergenic |
| TTGATTTGTGACCTTAGTAG+TGG | 0.562901 | 1:-100999923 | None:intergenic |
| AAAGAGAACCAATGCAACAA+TGG | 0.584299 | 1:+100999708 | MsG0180006174.01.T01:CDS |
| ATGTTATTCTATGATGGCCA+TGG | 0.591384 | 1:-100999837 | None:intergenic |
| GGTGTATGTAGCTAGTGAAG+AGG | 0.591556 | 1:+101000170 | MsG0180006174.01.T01:CDS |
| TAAAATACACGTCATACAGA+AGG | 0.595028 | 1:-101000416 | None:intergenic |
| AGAAACTTGGAAAAGCAGAA+GGG | 0.603723 | 1:+101000148 | MsG0180006174.01.T01:CDS |
| TCACATGTTGCAGTTATTGG+TGG | 0.626055 | 1:+101000294 | MsG0180006174.01.T01:CDS |
| TGACAATTGCTAGAAGAGAA+GGG | 0.634492 | 1:-100999738 | None:intergenic |
| AGGAAAAGAGAAACCAATGG+TGG | 0.644162 | 1:-101000034 | None:intergenic |
| GAAACTTGGAAAAGCAGAAG+GGG | 0.645048 | 1:+101000149 | MsG0180006174.01.T01:CDS |
| AGTAGGAAAAGAGAAACCAA+TGG | 0.647659 | 1:-101000037 | None:intergenic |
| ATTACCAAGAATTCTAGCTG+AGG | 0.661099 | 1:-100999797 | None:intergenic |
| CCAACCCCTAATCACTACCA+TGG | 0.665385 | 1:+100999820 | MsG0180006174.01.T01:CDS |
| CAGCATAGCCATTAGCACCA+TGG | 0.679074 | 1:-101000329 | None:intergenic |
| ATGGCCATGGTAGTGATTAG+GGG | 0.679512 | 1:-100999824 | None:intergenic |
| ATCAGTAACATCGATCGACG+AGG | 0.719628 | 1:+101000089 | MsG0180006174.01.T01:CDS |
| GGTGGTACTGGAAAGTACCA+TGG | 0.742516 | 1:+101000312 | MsG0180006174.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAAAAATGTTATTCTATGA+TGG | - | Chr1:100999846-100999865 | None:intergenic | 15.0% |
| ! | GGTTTCTCTGTATTAATATT+AGG | - | Chr1:100999901-100999920 | None:intergenic | 25.0% |
| ! | TCTGTATTAATATTAGGACT+AGG | - | Chr1:100999895-100999914 | None:intergenic | 25.0% |
| GAAGATGAACTAAAGAAACT+TGG | + | Chr1:101000135-101000154 | MsG0180006174.01.T01:CDS | 30.0% | |
| TAAAATACACGTCATACAGA+AGG | - | Chr1:101000419-101000438 | None:intergenic | 30.0% | |
| TTAAGGTTGTGGATAAAGTA+GGG | + | Chr1:101000355-101000374 | MsG0180006174.01.T01:CDS | 30.0% | |
| ! | AGCTGAGGAAAATTTGTTTA+TGG | - | Chr1:100999785-100999804 | None:intergenic | 30.0% |
| !!! | ATGACAGTGAGTTTTTTGAA+GGG | + | Chr1:101000216-101000235 | MsG0180006174.01.T01:CDS | 30.0% |
| !!! | TATGACAGTGAGTTTTTTGA+AGG | + | Chr1:101000215-101000234 | MsG0180006174.01.T01:CDS | 30.0% |
| AAAGAGAACCAATGCAACAA+TGG | + | Chr1:100999708-100999727 | MsG0180006174.01.T01:CDS | 35.0% | |
| AAGAAACTTGGAAAAGCAGA+AGG | + | Chr1:101000147-101000166 | MsG0180006174.01.T01:CDS | 35.0% | |
| AGAAACTTGGAAAAGCAGAA+GGG | + | Chr1:101000148-101000167 | MsG0180006174.01.T01:CDS | 35.0% | |
| AGTAGGAAAAGAGAAACCAA+TGG | - | Chr1:101000040-101000059 | None:intergenic | 35.0% | |
| ATGTTATTCTATGATGGCCA+TGG | - | Chr1:100999840-100999859 | None:intergenic | 35.0% | |
| GTTAAGGTTGTGGATAAAGT+AGG | + | Chr1:101000354-101000373 | MsG0180006174.01.T01:CDS | 35.0% | |
| TAATGGCTATGCTGTTGTTA+AGG | + | Chr1:101000338-101000357 | MsG0180006174.01.T01:CDS | 35.0% | |
| TGACAATTGCTAGAAGAGAA+GGG | - | Chr1:100999741-100999760 | None:intergenic | 35.0% | |
| TTTGGAGGAAAAAATCCTAG+AGG | - | Chr1:100999964-100999983 | None:intergenic | 35.0% | |
| ! | ATTACCAAGAATTCTAGCTG+AGG | - | Chr1:100999800-100999819 | None:intergenic | 35.0% |
| ! | GGAAAAAATCCTAGAGGTTT+TGG | - | Chr1:100999958-100999977 | None:intergenic | 35.0% |
| ! | TTACCTTTTCCAAAACCTCT+AGG | + | Chr1:100999946-100999965 | MsG0180006174.01.T01:CDS | 35.0% |
| ! | TTTTCCTCAGCTAGAATTCT+TGG | + | Chr1:100999793-100999812 | MsG0180006174.01.T01:CDS | 35.0% |
| !! | ATTGTTGCATTGGTTCTCTT+TGG | - | Chr1:100999709-100999728 | None:intergenic | 35.0% |
| !! | GTTTGAGTTGATTGTGGAAT+TGG | - | Chr1:100999997-101000016 | None:intergenic | 35.0% |
| !! | GTTTGTGTTTGAGTTGATTG+TGG | - | Chr1:101000003-101000022 | None:intergenic | 35.0% |
| !! | TTGATTTGTGACCTTAGTAG+TGG | - | Chr1:100999926-100999945 | None:intergenic | 35.0% |
| !!! | AATCCTAGAGGTTTTGGAAA+AGG | - | Chr1:100999952-100999971 | None:intergenic | 35.0% |
| !!! | CTAGGATTTTTTCCTCCAAA+TGG | + | Chr1:100999964-100999983 | MsG0180006174.01.T01:CDS | 35.0% |
| !!! | GATTTTTTCCTCCAAATGGA+CGG | + | Chr1:100999968-100999987 | MsG0180006174.01.T01:CDS | 35.0% |
| !!! | TGAGACTTTTTGGAGTTCAT+AGG | + | Chr1:101000256-101000275 | MsG0180006174.01.T01:CDS | 35.0% |
| ACACTTGATCTTTCCACCAT+TGG | + | Chr1:101000021-101000040 | MsG0180006174.01.T01:CDS | 40.0% | |
| AGAGAAACCTTCCACTACTA+AGG | + | Chr1:100999912-100999931 | MsG0180006174.01.T01:CDS | 40.0% | |
| AGGAAAAGAGAAACCAATGG+TGG | - | Chr1:101000037-101000056 | None:intergenic | 40.0% | |
| CTATGCTGTTGTTAAGGTTG+TGG | + | Chr1:101000344-101000363 | MsG0180006174.01.T01:CDS | 40.0% | |
| CTGACAATTGCTAGAAGAGA+AGG | - | Chr1:100999742-100999761 | None:intergenic | 40.0% | |
| CTTCTCTTCTAGCAATTGTC+AGG | + | Chr1:100999740-100999759 | MsG0180006174.01.T01:CDS | 40.0% | |
| GAAACTTGGAAAAGCAGAAG+GGG | + | Chr1:101000149-101000168 | MsG0180006174.01.T01:CDS | 40.0% | |
| GAGTCACATGTTGCAGTTAT+TGG | + | Chr1:101000291-101000310 | MsG0180006174.01.T01:CDS | 40.0% | |
| GCATTTCAAGAGCTGGAATT+TGG | + | Chr1:101000066-101000085 | MsG0180006174.01.T01:CDS | 40.0% | |
| TATGTAGCTAGTGAAGAGGA+TGG | + | Chr1:101000174-101000193 | MsG0180006174.01.T01:CDS | 40.0% | |
| TCACATGTTGCAGTTATTGG+TGG | + | Chr1:101000294-101000313 | MsG0180006174.01.T01:CDS | 40.0% | |
| TCACTGTCATAGCAACCATA+TGG | - | Chr1:101000206-101000225 | None:intergenic | 40.0% | |
| TCTTGAAATGCTGCTCTAGT+AGG | - | Chr1:101000057-101000076 | None:intergenic | 40.0% | |
| TTGAAGGGTGAGTATGAAGA+CGG | + | Chr1:101000231-101000250 | MsG0180006174.01.T01:CDS | 40.0% | |
| ! | TTTGTGACCTTAGTAGTGGA+AGG | - | Chr1:100999922-100999941 | None:intergenic | 40.0% |
| AGAGGATGGAAGTAGCCATA+TGG | + | Chr1:101000188-101000207 | MsG0180006174.01.T01:CDS | 45.0% | |
| ATCAGTAACATCGATCGACG+AGG | + | Chr1:101000089-101000108 | MsG0180006174.01.T01:CDS | 45.0% | |
| ATTGGAATCCGTCCATTTGG+AGG | - | Chr1:100999979-100999998 | None:intergenic | 45.0% | |
| GACGAGGAACTACTAGTTGA+AGG | + | Chr1:101000105-101000124 | MsG0180006174.01.T01:CDS | 45.0% | |
| GGAATTGGAATCCGTCCATT+TGG | - | Chr1:100999982-101000001 | None:intergenic | 45.0% | |
| GGTGTATGTAGCTAGTGAAG+AGG | + | Chr1:101000170-101000189 | MsG0180006174.01.T01:CDS | 45.0% | |
| GTAGGGTCTAGTACAGTAGA+AGG | + | Chr1:101000372-101000391 | MsG0180006174.01.T01:CDS | 45.0% | |
| TAGAGCAGCATTTCAAGAGC+TGG | + | Chr1:101000059-101000078 | MsG0180006174.01.T01:CDS | 45.0% | |
| TGAAGGGTGAGTATGAAGAC+GGG | + | Chr1:101000232-101000251 | MsG0180006174.01.T01:CDS | 45.0% | |
| TGCTAGAAGAGAAGGGAGAT+TGG | - | Chr1:100999734-100999753 | None:intergenic | 45.0% | |
| ! | ATGGCCATGGTAGTGATTAG+GGG | - | Chr1:100999827-100999846 | None:intergenic | 45.0% |
| ! | GAGATTGGCCATTGTTGCAT+TGG | - | Chr1:100999719-100999738 | None:intergenic | 45.0% |
| ! | GATGGCCATGGTAGTGATTA+GGG | - | Chr1:100999828-100999847 | None:intergenic | 45.0% |
| ! | GTTGCAGTTATTGGTGGTAC+TGG | + | Chr1:101000300-101000319 | MsG0180006174.01.T01:CDS | 45.0% |
| ! | TGATGGCCATGGTAGTGATT+AGG | - | Chr1:100999829-100999848 | None:intergenic | 45.0% |
| !! | GGAAAGTACCATGGTGCTAA+TGG | + | Chr1:101000321-101000340 | MsG0180006174.01.T01:CDS | 45.0% |
| !!! | GAAGACGGGTTGAGACTTTT+TGG | + | Chr1:101000246-101000265 | MsG0180006174.01.T01:CDS | 45.0% |
| CAGCATAGCCATTAGCACCA+TGG | - | Chr1:101000332-101000351 | None:intergenic | 50.0% | |
| CCAACCCCTAATCACTACCA+TGG | + | Chr1:100999820-100999839 | MsG0180006174.01.T01:CDS | 50.0% | |
| ! | CCATGGTAGTGATTAGGGGT+TGG | - | Chr1:100999823-100999842 | None:intergenic | 50.0% |
| !! | GGTGGTACTGGAAAGTACCA+TGG | + | Chr1:101000312-101000331 | MsG0180006174.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 100999703 | 101000443 | 100999703 | ID=MsG0180006174.01;Name=MsG0180006174.01 |
| Chr1 | mRNA | 100999703 | 101000443 | 100999703 | ID=MsG0180006174.01.T01;Parent=MsG0180006174.01;Name=MsG0180006174.01.T01;_AED=0.34;_eAED=0.34;_QI=0|-1|0|1|-1|1|1|0|246 |
| Chr1 | exon | 100999703 | 101000443 | 100999703 | ID=MsG0180006174.01.T01:exon:12777;Parent=MsG0180006174.01.T01 |
| Chr1 | CDS | 100999703 | 101000443 | 100999703 | ID=MsG0180006174.01.T01:cds;Parent=MsG0180006174.01.T01 |
| Gene Sequence |
| Protein sequence |
