AlfalfaGEDB | MsG0480022428.01

M. sativa cultivar ZhongmuNo.1 / MsG0480022428.01

KEGG: -
GO: GO:0004672 GO:0005524 GO:0006468
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022428.01.T01	KEH30484.1	94.167	360	21	0	1	360	1	360	0	662
MsG0480022428.01.T02	XP_003607181.2	96.899	258	8	0	2	259	387	644	2.63E-180	520

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022428.01.T01	O04086	47.887	71	36	1	288	357	282	352	3.03E-11	68.2
MsG0480022428.01.T02	Q5XF57	46.875	192	97	2	5	196	355	541	6.13E-58	196

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022428.01.T01	A0A072UL58	94.167	360	21	0	1	360	1	360	0.0	662
MsG0480022428.01.T02	G7JMD5	96.899	258	8	0	2	259	387	644	1.26e-180	520

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047967.01	MsG0480022428.01	-0.821184	4.621556e-53	3.733581e-50
MsG0080048134.01	MsG0480022428.01	0.824001	1.022203e-53	8.941913e-51
MsG0080048347.01	MsG0480022428.01	0.805596	1.236530e-49	6.568441e-47
MsG0080048595.01	MsG0480022428.01	0.808703	2.718693e-50	1.565227e-47
MsG0080048694.01	MsG0480022428.01	-0.808992	2.357594e-50	1.367808e-47
MsG0080048695.01	MsG0480022428.01	-0.809653	1.701657e-50	1.004853e-47
MsG0080048889.01	MsG0480022428.01	0.900586	6.318898e-78	8.228008e-74
MsG0080048980.01	MsG0480022428.01	0.854113	1.527656e-61	3.376025e-58
MsG0080048992.01	MsG0480022428.01	-0.821598	3.707327e-53	3.029543e-50
MsG0080049008.01	MsG0480022428.01	0.844240	8.545347e-59	1.370986e-55

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022428.01.T01	MTR_4g073140	94.167	360	21	0	1	360	1	360	0.0	662
MsG0480022428.01.T01	MTR_4g073140	94.167	360	21	0	1	360	1	360	0.0	660
MsG0480022428.01.T01	MTR_4g073140	94.167	360	21	0	1	360	1	360	0.0	659
MsG0480022428.01.T01	MTR_3g117850	53.406	367	118	9	1	353	1	328	2.25e-116	351
MsG0480022428.01.T01	MTR_3g117850	48.092	262	86	7	105	353	5	229	2.98e-64	213
MsG0480022428.01.T01	MTR_1g029690	34.223	412	211	10	6	363	12	417	8.10e-53	186
MsG0480022428.01.T01	MTR_3g096555	34.359	390	190	12	6	363	11	366	3.63e-48	172
MsG0480022428.01.T01	MTR_3g096555	33.416	404	217	11	6	363	11	408	2.10e-47	171
MsG0480022428.01.T01	MTR_3g117850	45.291	223	73	6	143	353	14	199	4.83e-46	164
MsG0480022428.01.T01	MTR_3g088640	42.857	147	69	3	4	135	11	157	4.22e-26	110

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022428.01.T01	AT2G16750	38.567	363	174	9	4	356	10	333	2.12e-64	216
MsG0480022428.01.T01	AT2G16750	38.567	363	174	9	4	356	10	333	2.12e-64	216
MsG0480022428.01.T01	AT5G63940	30.562	409	225	11	7	362	21	423	9.10e-43	158
MsG0480022428.01.T01	AT4G35030	38.710	186	80	6	178	356	2	160	7.73e-28	111
MsG0480022428.01.T01	AT4G35030	38.710	186	80	6	178	356	2	160	7.73e-28	111
MsG0480022428.01.T01	AT4G35030	38.710	186	80	6	178	356	2	160	8.67e-28	112
MsG0480022428.01.T01	AT1G77280	35.714	182	111	2	186	363	287	466	1.14e-27	115
MsG0480022428.01.T01	AT1G77280	42.069	145	68	3	6	135	19	162	1.94e-25	108
MsG0480022428.01.T01	AT1G77280	35.714	182	111	2	186	363	287	466	1.14e-27	115
MsG0480022428.01.T01	AT1G77280	42.069	145	68	3	6	135	19	162	1.94e-25	108

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Find 100 sgRNAs with CRISPR-Local

Find 235 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAGGTTTGTGTTGGTT+GGG	0.444879	4:+72488813	MsG0480022428.01.T01:CDS
AAACATTTGACGGATCAGAA+AGG	0.597247	4:+72490069	MsG0480022428.01.T01:CDS
AAGATTCAAGAATCGTCTTC+GGG	0.529437	4:+72489558	MsG0480022428.01.T01:CDS
AATATCCCTTCTAGGCCTTT+TGG	0.243251	4:-72490362	None:intergenic
AATGGATGTAGGAAAGAACA+AGG	0.609430	4:+72490958	MsG0480022428.01.T01:intron
AATTGAGAAGCTGTCTGCTA+TGG	0.541023	4:-72488850	None:intergenic
ACACAATGACAGTTGAAAAG+AGG	0.481746	4:+72488798	MsG0480022428.01.T01:exon
ACAGCAATAACACAATCACC+AGG	0.707375	4:-72488896	None:intergenic
AGATGTCTGAATTCGATTTA+CGG	0.396484	4:-72490160	None:intergenic
AGCTTAGAGGAAAATCTACA+TGG	0.638566	4:+72490848	MsG0480022428.01.T01:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAACAAGAGCCTAATAGA+TGG	+	Chr4:72489144-72489163	MsG0480022428.01.T01:CDS	30.0%
AAAACATGTTTCAATTGCAG+AGG	+	Chr4:72489470-72489489	MsG0480022428.01.T01:intron	30.0%
AAAACCAATAATAGAGAGTG+GGG	+	Chr4:72491772-72491791	MsG0480022428.01.T01:three_prime_UTR	30.0%
AAAGTTGTCGAGACTAAATT+TGG	+	Chr4:72491230-72491249	MsG0480022428.01.T01:intron	30.0%
ACTTCGTCCTAAATTTAATC+AGG	+	Chr4:72491898-72491917	MsG0480022428.01.T01:three_prime_UTR	30.0%
AGATACTGAAGATCCTAAAA+GGG	+	Chr4:72492111-72492130	MsG0480022428.01.T01:intron	30.0%
AGATGTCTGAATTCGATTTA+CGG	-	Chr4:72490163-72490182	None:intergenic	30.0%
AGTTGTACGATGTCTAAATT+TGG	-	Chr4:72491205-72491224	None:intergenic	30.0%
CAAAACATTACTCAACTACT+TGG	+	Chr4:72490770-72490789	MsG0480022428.01.T01:CDS	30.0%
CAAAACCAATAATAGAGAGT+GGG	+	Chr4:72491771-72491790	MsG0480022428.01.T01:three_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	72488704	72492382	72488704	ID=MsG0480022428.01;Name=MsG0480022428.01
Chr4	mRNA	72488704	72492382	72488704	ID=MsG0480022428.01.T01;Parent=MsG0480022428.01;Name=MsG0480022428.01.T01;_AED=0.25;_eAED=0.25;_QI=99\|1\|1\|1\|0.88\|0.8\|10\|878\|363
Chr4	exon	72490969	72491132	72490969	ID=MsG0480022428.01.T01:exon:20576;Parent=MsG0480022428.01.T01
Chr4	exon	72490622	72490869	72490622	ID=MsG0480022428.01.T01:exon:20575;Parent=MsG0480022428.01.T01
Chr4	exon	72489976	72490458	72489976	ID=MsG0480022428.01.T01:exon:20574;Parent=MsG0480022428.01.T01
Chr4	exon	72489490	72489605	72489490	ID=MsG0480022428.01.T01:exon:20573;Parent=MsG0480022428.01.T01
Chr4	exon	72489292	72489386	72489292	ID=MsG0480022428.01.T01:exon:20572;Parent=MsG0480022428.01.T01
Chr4	exon	72489133	72489206	72489133	ID=MsG0480022428.01.T01:exon:20571;Parent=MsG0480022428.01.T01
Chr4	exon	72488704	72488944	72488704	ID=MsG0480022428.01.T01:exon:20570;Parent=MsG0480022428.01.T01
Chr4	five_prime_UTR	72488704	72488802	72488704	ID=MsG0480022428.01.T01:five_prime_utr;Parent=MsG0480022428.01.T01
Chr4	CDS	72488803	72488944	72488803	ID=MsG0480022428.01.T01:cds;Parent=MsG0480022428.01.T01
Chr4	CDS	72489133	72489206	72489133	ID=MsG0480022428.01.T01:cds;Parent=MsG0480022428.01.T01
Chr4	CDS	72489292	72489386	72489292	ID=MsG0480022428.01.T01:cds;Parent=MsG0480022428.01.T01
Chr4	CDS	72489490	72489605	72489490	ID=MsG0480022428.01.T01:cds;Parent=MsG0480022428.01.T01
Chr4	CDS	72489976	72490458	72489976	ID=MsG0480022428.01.T01:cds;Parent=MsG0480022428.01.T01
Chr4	CDS	72490622	72490803	72490622	ID=MsG0480022428.01.T01:cds;Parent=MsG0480022428.01.T01
Chr4	three_prime_UTR	72490804	72490869	72490804	ID=MsG0480022428.01.T01:three_prime_utr;Parent=MsG0480022428.01.T01
Chr4	three_prime_UTR	72490969	72491132	72490969	ID=MsG0480022428.01.T01:three_prime_utr;Parent=MsG0480022428.01.T01
Chr4	three_prime_UTR	72491344	72491571	72491344	ID=MsG0480022428.01.T01:three_prime_utr;Parent=MsG0480022428.01.T01
Chr4	three_prime_UTR	72491770	72491919	72491770	ID=MsG0480022428.01.T01:three_prime_utr;Parent=MsG0480022428.01.T01
Chr4	three_prime_UTR	72492113	72492382	72492113	ID=MsG0480022428.01.T01:three_prime_utr;Parent=MsG0480022428.01.T01
Chr4	mRNA	72490959	72492382	72490959	ID=MsG0480022428.01.T02;Parent=MsG0480022428.01;Name=MsG0480022428.01.T02;_AED=0.40;_eAED=0.40;_QI=0\|1\|0.75\|1\|1\|1\|4\|42\|259
Chr4	exon	72490959	72491132	72490959	ID=MsG0480022428.01.T02:exon:20580;Parent=MsG0480022428.01.T02
Chr4	CDS	72490959	72491132	72490959	ID=MsG0480022428.01.T02:cds;Parent=MsG0480022428.01.T02
Chr4	CDS	72491344	72491571	72491344	ID=MsG0480022428.01.T02:cds;Parent=MsG0480022428.01.T02
Chr4	CDS	72491770	72491919	72491770	ID=MsG0480022428.01.T02:cds;Parent=MsG0480022428.01.T02
Chr4	CDS	72492113	72492340	72492113	ID=MsG0480022428.01.T02:cds;Parent=MsG0480022428.01.T02
Chr4	three_prime_UTR	72492341	72492382	72492341	ID=MsG0480022428.01.T02:three_prime_utr;Parent=MsG0480022428.01.T02

Gene Sequence

>MsG0480022428.01.T01

ATGACAGTTGAAAAGAGGTTTGTGTTGGTTGGGATTAGAATAGATAGCCATAGCAGACAGCTTCTCAATTGGGCTCTTGTTAAAGTTGCTGAACCTGGTGATTGTGTTATTGCTGTTCATGTAGTTAAAACTAAAAGCTCAGATTATGTTTCTAAAAACAAGAGCCTAATAGATGGATATTTAGAGGTTTATGAAGGCTTGTGTGATGTGAAAAAGGTTGGTCTTTCTAGTCAAATCATAACGGGAAAGTCCATTAAAAATATCCTTGTTAGAGAAGCAAAAAACCAAGGTGCTTTGGCTCTAGTGGTAGGAGGTAAAGCTGCCACGGCTAAATATTGCGCCAAACGTCTTCCACCCACCACCAATGTTCTTGCCATCCAAGATTCAAGAATCGTCTTCGGGAGGTGCAATAATAAACGACCACCAGGTAGTCTTATATTGGATCCAAGACCAAGTTTAACAAGCATAGATAACCTGAGTGAGAAATCACCACACCAAAACACCAAGGAATCGAAAGAAGAAACATTTGACGGATCAGAAAGGCAAAAGTCACGATCGATATCCATGTTTGCAGACGATCCTTATAAGCGGAAGCTTGGATGGCCTCTGCTCCGTAAATCGAATTCAGACATCTCACAAAGTCATCATGGAAGGGGTATTTCAGTAGTACAATGGGTAATGAGCTTACCAGATCGTTCACCACGTAGTAGTCCTCAATCATCTTCCTCAGACGAAAATCCATTCGAAAGGAGCATAAGTGACCTTCTTGAGGATGAGAGCTTCAAAAATCATTTACCTCCATCAGTTGTACTCCCAAAAGGCCTAGAAGGGATATTAAATGTAAATTCACTCAATTGCAAATGGCTCAGCCTTGAGGCTCTCAAGTCTTGTACCAGTCAATTCTCTTCAGAAAATTTGATTGGGAAAGGAGGAAGCAACCGTGTGTACAAAGGAATTCTCCCTGATGGAAAACCAATTGCAGTTAAAGTATTGCGATCATCAAAAGAAGCATGGAAAGATTTTGCCTTCGAGATGGAAATAATATCATCATTGAATCACAAAACATTACTCAACTACTTGGAATTTGCGTAG

>MsG0480022428.01.T02

ATGGATGTAGGAAAGAACAAGGATGGATCTCTTTTGTCATGGGAAGTGAGATTTAATGTAGCTGTTGGAGTAGCTGAGGCCCTTGATTACCTACACACAGAAGCTTCGAAGCATGTCATACATAGAGATGTCAAGTCTTCAAACATTCTTCTTTCCAAGGAGCTTGAGCCACAATTGTCTGATTTTGGACTTGCAATATGGGGACCAACAACAACATCCTTTTTGACACAAGAAGATGTAGTAGGAACATTTGGTTATCTTGCTCCTGAATACTTCATGTATGGAAAAGTTAGTGACAAGATAGATGTCTATGCCTTTGGTGTAGTACTACTTGAACTACTATCAGGTAGGGAACCAATCAGCTCTGAACCTTGCAAAGGACAGGAGAGCTTGGTAGCTTGGGCAAAACCAATAATAGAGAGTGGGGATATTAACGGTTTGTTGGACCCAAAATTAGAAGGGAAGTTTGATGAGACTCAAATGAAAAGAATGGTTTTGGCAGCGTCTTTATGCATCGCACGGGCTGCTAGACTTCGTCCTAAATTTAATCAGATACTGAAGATCCTAAAAGGGAACAATGAAGATGAATATTGCTTCAATGAGTATTCAGAAAATGAAGAAAACATTGATGATGAAGTGTATCCAAATTCAAGTACAGAGTTACACCTATCTCTTGCATTACTTGGTGTTGATGATGACAGTACATCATGTAGTAGTACAGACCACAACTACAGTGAGAATTTGAAAGATCAATGGAGCATGTCTTTGAGTTTTAACTAG

Protein sequence

>MsG0480022428.01.T01

MTVEKRFVLVGIRIDSHSRQLLNWALVKVAEPGDCVIAVHVVKTKSSDYVSKNKSLIDGYLEVYEGLCDVKKVGLSSQIITGKSIKNILVREAKNQGALALVVGGKAATAKYCAKRLPPTTNVLAIQDSRIVFGRCNNKRPPGSLILDPRPSLTSIDNLSEKSPHQNTKESKEETFDGSERQKSRSISMFADDPYKRKLGWPLLRKSNSDISQSHHGRGISVVQWVMSLPDRSPRSSPQSSSSDENPFERSISDLLEDESFKNHLPPSVVLPKGLEGILNVNSLNCKWLSLEALKSCTSQFSSENLIGKGGSNRVYKGILPDGKPIAVKVLRSSKEAWKDFAFEMEIISSLNHKTLLNYLEFA*

>MsG0480022428.01.T02

MDVGKNKDGSLLSWEVRFNVAVGVAEALDYLHTEASKHVIHRDVKSSNILLSKELEPQLSDFGLAIWGPTTTSFLTQEDVVGTFGYLAPEYFMYGKVSDKIDVYAFGVVLLELLSGREPISSEPCKGQESLVAWAKPIIESGDINGLLDPKLEGKFDETQMKRMVLAASLCIARAARLRPKFNQILKILKGNNEDEYCFNEYSENEENIDDEVYPNSSTELHLSLALLGVDDDSTSCSSTDHNYSENLKDQWSMSLSFN*