Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012565.01.T01 | XP_003598997.1 | 91.011 | 623 | 52 | 1 | 6 | 628 | 2 | 620 | 0 | 1137 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012565.01.T01 | Q9FNR3 | 42.265 | 627 | 342 | 7 | 14 | 627 | 20 | 639 | 1.50E-158 | 472 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012565.01.T01 | G7IZB2 | 91.011 | 623 | 52 | 1 | 6 | 628 | 2 | 620 | 0.0 | 1137 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000599.01 | MsG0380012565.01 | 0.835570 | 1.553953e-56 | 1.907690e-53 |
| MsG0180000782.01 | MsG0380012565.01 | 0.813253 | 2.810470e-51 | 1.826040e-48 |
| MsG0180000977.01 | MsG0380012565.01 | 0.809104 | 2.230978e-50 | 1.298291e-47 |
| MsG0180004246.01 | MsG0380012565.01 | 0.819632 | 1.048840e-52 | 8.114740e-50 |
| MsG0180004765.01 | MsG0380012565.01 | 0.830729 | 2.492273e-55 | 2.648469e-52 |
| MsG0180005730.01 | MsG0380012565.01 | 0.801366 | 9.314625e-49 | 4.440387e-46 |
| MsG0180005953.01 | MsG0380012565.01 | 0.841314 | 5.123732e-58 | 7.498754e-55 |
| MsG0180006180.01 | MsG0380012565.01 | 0.820611 | 6.258614e-53 | 4.975580e-50 |
| MsG0280006309.01 | MsG0380012565.01 | 0.808122 | 3.615987e-50 | 2.050441e-47 |
| MsG0280011255.01 | MsG0380012565.01 | 0.805206 | 1.493140e-49 | 7.852518e-47 |
| MsG0280011343.01 | MsG0380012565.01 | 0.804864 | 1.760546e-49 | 9.178411e-47 |
| MsG0380012312.01 | MsG0380012565.01 | 0.815140 | 1.076805e-51 | 7.363137e-49 |
| MsG0380012563.01 | MsG0380012565.01 | 0.838774 | 2.355186e-57 | 3.187019e-54 |
| MsG0380012564.01 | MsG0380012565.01 | 0.885407 | 8.349101e-72 | 5.724435e-68 |
| MsG0380012565.01 | MsG0380014386.01 | 0.805985 | 1.024537e-49 | 5.497317e-47 |
| MsG0380012565.01 | MsG0380015847.01 | -0.822616 | 2.153402e-53 | 1.811765e-50 |
| MsG0380012565.01 | MsG0380015884.01 | 0.806799 | 6.901523e-50 | 3.780779e-47 |
| MsG0380012565.01 | MsG0380015885.01 | 0.827907 | 1.206907e-54 | 1.181154e-51 |
| MsG0380012565.01 | MsG0380015891.01 | 0.832291 | 1.027859e-55 | 1.143714e-52 |
| MsG0380012565.01 | MsG0380017202.01 | 0.804130 | 2.503815e-49 | 1.280785e-46 |
| MsG0380012565.01 | MsG0480018653.01 | 0.807518 | 4.861839e-50 | 2.713652e-47 |
| MsG0380012565.01 | MsG0480018677.01 | 0.805398 | 1.360650e-49 | 7.191139e-47 |
| MsG0380012565.01 | MsG0480020024.01 | 0.808528 | 2.962561e-50 | 1.697947e-47 |
| MsG0380012565.01 | MsG0480020486.01 | 0.814597 | 1.420864e-51 | 9.574928e-49 |
| MsG0380012565.01 | MsG0480022355.01 | 0.822816 | 1.934822e-53 | 1.636961e-50 |
| MsG0380012565.01 | MsG0480022537.01 | 0.806039 | 9.981696e-50 | 5.362988e-47 |
| MsG0380012565.01 | MsG0480022918.01 | 0.817057 | 4.017906e-52 | 2.895738e-49 |
| MsG0380012565.01 | MsG0580025827.01 | 0.816041 | 6.782538e-52 | 4.754401e-49 |
| MsG0380012565.01 | MsG0580026173.01 | 0.805570 | 1.252726e-49 | 6.649864e-47 |
| MsG0380012565.01 | MsG0580027955.01 | 0.825580 | 4.339116e-54 | 3.970937e-51 |
| MsG0380012565.01 | MsG0580027980.01 | 0.818451 | 1.947112e-52 | 1.458175e-49 |
| MsG0380012565.01 | MsG0680030680.01 | 0.816288 | 5.974350e-52 | 4.216264e-49 |
| MsG0380012565.01 | MsG0680030838.01 | 0.823929 | 1.062734e-53 | 9.278728e-51 |
| MsG0380012565.01 | MsG0680030895.01 | 0.827873 | 1.229716e-54 | 1.202314e-51 |
| MsG0380012565.01 | MsG0680030905.01 | 0.813886 | 2.039291e-51 | 1.348051e-48 |
| MsG0380012565.01 | MsG0680032254.01 | 0.808689 | 2.736889e-50 | 1.575125e-47 |
| MsG0380012565.01 | MsG0680032413.01 | 0.816921 | 4.310709e-52 | 3.095128e-49 |
| MsG0380012565.01 | MsG0680034013.01 | 0.807200 | 5.675911e-50 | 3.141944e-47 |
| MsG0380012565.01 | MsG0680035596.01 | 0.814153 | 1.781073e-51 | 1.185864e-48 |
| MsG0380012565.01 | MsG0680035707.01 | 0.810532 | 1.099784e-50 | 6.645851e-48 |
| MsG0380012565.01 | MsG0780039336.01 | 0.804378 | 2.223216e-49 | 1.144541e-46 |
| MsG0380012565.01 | MsG0780039438.01 | 0.821353 | 4.223844e-53 | 3.428193e-50 |
| MsG0380012565.01 | MsG0780040278.01 | 0.832108 | 1.140657e-55 | 1.262467e-52 |
| MsG0380012565.01 | MsG0780040431.01 | 0.804033 | 2.622610e-49 | 1.338272e-46 |
| MsG0380012565.01 | MsG0880042416.01 | 0.823374 | 1.433713e-53 | 1.232133e-50 |
| MsG0380012565.01 | MsG0880042576.01 | 0.808656 | 2.782714e-50 | 1.600065e-47 |
| MsG0380012565.01 | MsG0880042622.01 | 0.804638 | 1.961871e-49 | 1.016775e-46 |
| MsG0380012565.01 | MsG0880043157.01 | 0.805124 | 1.552829e-49 | 8.149570e-47 |
| MsG0380012565.01 | MsG0880044538.01 | 0.803655 | 3.142287e-49 | 1.587870e-46 |
| MsG0380012565.01 | MsG0880044892.01 | 0.817310 | 3.523901e-52 | 2.557207e-49 |
| MsG0380012565.01 | MsG0880045668.01 | 0.812460 | 4.193345e-51 | 2.667899e-48 |
| MsG0380012565.01 | MsG0880045765.01 | 0.814862 | 1.241116e-51 | 8.423219e-49 |
| MsG0380012565.01 | MsG0880045783.01 | 0.801232 | 9.924562e-49 | 4.715066e-46 |
| MsG0380012565.01 | MsG0880045992.01 | 0.816700 | 4.831723e-52 | 3.448171e-49 |
| MsG0380012565.01 | MsG0880046334.01 | -0.803076 | 4.142131e-49 | 2.062135e-46 |
| MsG0380012565.01 | MsG0880047394.01 | 0.810798 | 9.636129e-51 | 5.864627e-48 |
| MsG0280006592.01 | MsG0380012565.01 | 0.806253 | 8.998852e-50 | 4.861546e-47 |
| MsG0280006914.01 | MsG0380012565.01 | 0.822339 | 2.498178e-53 | 2.085138e-50 |
| MsG0280006937.01 | MsG0380012565.01 | 0.840853 | 6.768249e-58 | 9.766374e-55 |
| MsG0280007877.01 | MsG0380012565.01 | 0.815111 | 1.092717e-51 | 7.466204e-49 |
| MsG0280008101.01 | MsG0380012565.01 | 0.811249 | 7.694900e-51 | 4.739462e-48 |
| MsG0280008899.01 | MsG0380012565.01 | 0.807904 | 4.023836e-50 | 2.268452e-47 |
| MsG0280009507.01 | MsG0380012565.01 | 0.800846 | 1.190068e-48 | 5.599036e-46 |
| MsG0280009757.01 | MsG0380012565.01 | 0.806305 | 8.774624e-50 | 4.746489e-47 |
| MsG0280009960.01 | MsG0380012565.01 | 0.801293 | 9.639310e-49 | 4.586715e-46 |
| MsG0280010216.01 | MsG0380012565.01 | 0.806769 | 7.003146e-50 | 3.833445e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0780036548.01 | MsG0380012565.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012565.01.T01 | MTR_3g025900 | 91.011 | 623 | 52 | 1 | 6 | 628 | 2 | 620 | 0.0 | 1137 |
| MsG0380012565.01.T01 | MTR_7g013770 | 40.625 | 672 | 351 | 8 | 1 | 627 | 9 | 677 | 7.42e-172 | 505 |
| MsG0380012565.01.T01 | MTR_4g103540 | 37.918 | 538 | 307 | 8 | 111 | 627 | 160 | 691 | 7.30e-114 | 356 |
| MsG0380012565.01.T01 | MTR_8g021330 | 34.835 | 577 | 328 | 11 | 83 | 622 | 46 | 611 | 1.56e-99 | 317 |
| MsG0380012565.01.T01 | MTR_7g026630 | 34.842 | 442 | 272 | 7 | 200 | 628 | 221 | 659 | 1.89e-85 | 281 |
| MsG0380012565.01.T01 | MTR_1g090620 | 30.511 | 567 | 330 | 13 | 83 | 614 | 102 | 639 | 8.24e-84 | 276 |
| MsG0380012565.01.T01 | MTR_5g073450 | 30.962 | 520 | 339 | 9 | 114 | 620 | 125 | 637 | 1.01e-82 | 273 |
| MsG0380012565.01.T01 | MTR_1g057560 | 30.534 | 524 | 315 | 8 | 113 | 614 | 127 | 623 | 1.53e-80 | 267 |
| MsG0380012565.01.T01 | MTR_6g033310 | 30.975 | 523 | 329 | 8 | 113 | 618 | 220 | 727 | 1.96e-77 | 261 |
| MsG0380012565.01.T01 | MTR_3g031130 | 32.880 | 441 | 279 | 6 | 189 | 620 | 191 | 623 | 7.19e-75 | 252 |
| MsG0380012565.01.T01 | MTR_5g073460 | 31.209 | 455 | 302 | 5 | 175 | 620 | 244 | 696 | 5.92e-68 | 234 |
| MsG0380012565.01.T01 | MTR_2g096230 | 30.443 | 542 | 341 | 11 | 80 | 615 | 69 | 580 | 7.70e-67 | 229 |
| MsG0380012565.01.T01 | MTR_1g067460 | 28.626 | 524 | 323 | 12 | 117 | 614 | 101 | 599 | 2.80e-59 | 209 |
| MsG0380012565.01.T01 | MTR_4g062330 | 28.599 | 514 | 341 | 9 | 116 | 615 | 102 | 603 | 2.80e-58 | 207 |
| MsG0380012565.01.T01 | MTR_1g067480 | 27.660 | 517 | 340 | 12 | 117 | 616 | 103 | 602 | 3.07e-58 | 206 |
| MsG0380012565.01.T01 | MTR_1g117090 | 28.986 | 552 | 334 | 9 | 113 | 614 | 123 | 666 | 1.54e-57 | 206 |
| MsG0380012565.01.T01 | MTR_5g073620 | 26.754 | 456 | 289 | 9 | 183 | 628 | 281 | 701 | 3.15e-43 | 167 |
| MsG0380012565.01.T01 | MTR_5g073620 | 28.994 | 169 | 113 | 3 | 452 | 614 | 899 | 1066 | 3.33e-11 | 67.0 |
| MsG0380012565.01.T01 | MTR_5g074130 | 25.799 | 438 | 298 | 9 | 189 | 614 | 251 | 673 | 2.01e-32 | 134 |
| MsG0380012565.01.T01 | MTR_5g073480 | 24.766 | 428 | 257 | 6 | 183 | 605 | 253 | 620 | 3.14e-32 | 132 |
| MsG0380012565.01.T01 | MTR_1g009620 | 24.117 | 651 | 403 | 18 | 7 | 627 | 73 | 662 | 8.19e-32 | 131 |
| MsG0380012565.01.T01 | MTR_3g464010 | 26.106 | 452 | 287 | 13 | 184 | 614 | 241 | 666 | 1.47e-29 | 124 |
| MsG0380012565.01.T01 | MTR_4g008260 | 28.424 | 387 | 245 | 12 | 251 | 623 | 278 | 646 | 5.87e-27 | 116 |
| MsG0380012565.01.T01 | MTR_1g017910 | 26.630 | 368 | 244 | 10 | 243 | 588 | 297 | 660 | 1.57e-25 | 112 |
| MsG0380012565.01.T01 | MTR_5g073770 | 30.460 | 174 | 119 | 1 | 183 | 354 | 194 | 367 | 2.57e-21 | 97.1 |
| MsG0380012565.01.T01 | MTR_5g073920 | 24.658 | 438 | 293 | 13 | 189 | 614 | 245 | 657 | 7.34e-21 | 97.8 |
| MsG0380012565.01.T01 | MTR_5g093530 | 25.868 | 317 | 201 | 8 | 275 | 589 | 258 | 542 | 3.63e-20 | 95.1 |
| MsG0380012565.01.T01 | MTR_0229s0040 | 31.522 | 184 | 109 | 4 | 448 | 618 | 31 | 210 | 4.64e-19 | 87.4 |
| MsG0380012565.01.T01 | MTR_5g073900 | 24.619 | 394 | 229 | 11 | 207 | 586 | 1 | 340 | 2.22e-16 | 81.6 |
| MsG0380012565.01.T01 | MTR_5g073780 | 28.402 | 169 | 114 | 3 | 452 | 614 | 54 | 221 | 6.26e-13 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012565.01.T01 | AT5G61010 | 42.265 | 627 | 342 | 7 | 14 | 627 | 20 | 639 | 1.53e-159 | 472 |
| MsG0380012565.01.T01 | AT5G61010 | 42.265 | 627 | 342 | 7 | 14 | 627 | 20 | 639 | 1.53e-159 | 472 |
| MsG0380012565.01.T01 | AT3G29400 | 37.462 | 662 | 360 | 13 | 8 | 627 | 9 | 658 | 3.18e-134 | 408 |
| MsG0380012565.01.T01 | AT5G50380 | 38.276 | 580 | 306 | 10 | 80 | 627 | 123 | 682 | 1.01e-127 | 392 |
| MsG0380012565.01.T01 | AT3G14090 | 35.019 | 534 | 304 | 8 | 116 | 614 | 86 | 611 | 7.02e-103 | 325 |
| MsG0380012565.01.T01 | AT1G54090 | 32.650 | 585 | 333 | 9 | 83 | 622 | 49 | 617 | 7.76e-94 | 302 |
| MsG0380012565.01.T01 | AT1G72470 | 33.390 | 587 | 329 | 10 | 83 | 622 | 56 | 627 | 2.90e-92 | 298 |
| MsG0380012565.01.T01 | AT5G03540 | 32.588 | 537 | 310 | 8 | 101 | 614 | 6 | 513 | 1.09e-88 | 285 |
| MsG0380012565.01.T01 | AT5G03540 | 31.777 | 557 | 326 | 9 | 83 | 614 | 101 | 628 | 3.27e-88 | 287 |
| MsG0380012565.01.T01 | AT5G03540 | 30.716 | 573 | 337 | 9 | 83 | 614 | 101 | 654 | 2.64e-85 | 280 |
| MsG0380012565.01.T01 | AT5G52340 | 31.061 | 528 | 323 | 10 | 101 | 614 | 121 | 621 | 5.03e-79 | 263 |
| MsG0380012565.01.T01 | AT5G58430 | 33.257 | 436 | 272 | 7 | 189 | 617 | 196 | 619 | 3.51e-76 | 255 |
| MsG0380012565.01.T01 | AT1G07000 | 28.483 | 646 | 372 | 16 | 8 | 617 | 6 | 597 | 4.45e-74 | 249 |
| MsG0380012565.01.T01 | AT5G13150 | 31.070 | 486 | 303 | 8 | 159 | 621 | 173 | 649 | 4.85e-69 | 237 |
| MsG0380012565.01.T01 | AT1G07725 | 29.381 | 565 | 347 | 13 | 83 | 628 | 72 | 603 | 6.20e-67 | 230 |
| MsG0380012565.01.T01 | AT5G13990 | 31.273 | 534 | 338 | 12 | 110 | 628 | 174 | 693 | 7.04e-67 | 232 |
| MsG0380012565.01.T01 | AT3G09520 | 28.942 | 539 | 348 | 10 | 97 | 616 | 84 | 606 | 5.30e-64 | 223 |
| MsG0380012565.01.T01 | AT2G28640 | 30.233 | 516 | 324 | 11 | 114 | 620 | 94 | 582 | 1.05e-63 | 221 |
| MsG0380012565.01.T01 | AT5G59730 | 30.275 | 545 | 341 | 11 | 95 | 617 | 72 | 599 | 1.50e-63 | 221 |
| MsG0380012565.01.T01 | AT5G59730 | 30.275 | 545 | 341 | 11 | 95 | 617 | 72 | 599 | 1.66e-63 | 221 |
| MsG0380012565.01.T01 | AT2G39380 | 28.627 | 517 | 343 | 8 | 117 | 618 | 107 | 612 | 1.11e-62 | 219 |
| MsG0380012565.01.T01 | AT3G55150 | 29.231 | 520 | 336 | 8 | 117 | 618 | 107 | 612 | 3.12e-59 | 209 |
| MsG0380012565.01.T01 | AT5G52350 | 28.226 | 496 | 321 | 9 | 134 | 615 | 102 | 576 | 8.89e-57 | 202 |
| MsG0380012565.01.T01 | AT2G28650 | 29.808 | 416 | 278 | 5 | 200 | 614 | 152 | 554 | 8.21e-50 | 182 |
| MsG0380012565.01.T01 | AT3G09530 | 25.465 | 538 | 369 | 9 | 95 | 614 | 77 | 600 | 2.44e-49 | 182 |
| MsG0380012565.01.T01 | AT4G31540 | 25.702 | 463 | 310 | 10 | 174 | 618 | 212 | 658 | 1.76e-33 | 136 |
| MsG0380012565.01.T01 | AT1G51640 | 24.777 | 448 | 291 | 14 | 197 | 615 | 196 | 626 | 1.69e-21 | 99.8 |
Find 86 sgRNAs with CRISPR-Local
Find 99 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTAATTTCTTTGGAGATTTC+TGG | 0.100464 | 3:+19869989 | MsG0380012565.01.T01:CDS |
| TGATCTACAATGTTATATTT+TGG | 0.148649 | 3:+19870329 | MsG0380012565.01.T01:CDS |
| TACCGAACTCCGAGATATTT+TGG | 0.173433 | 3:-19869401 | None:intergenic |
| GGAGAAACAAAGCTGATAAA+TGG | 0.222224 | 3:-19869380 | None:intergenic |
| AAAACCCTATGTAATTTCTT+TGG | 0.238723 | 3:+19869979 | MsG0380012565.01.T01:CDS |
| CATCGAAAATGTGCTTAAAT+TGG | 0.243970 | 3:+19869240 | MsG0380012565.01.T01:CDS |
| CATCGACAATTAGAAGATTA+AGG | 0.252964 | 3:-19869750 | None:intergenic |
| GCCATATCTTCATCAGTTCT+AGG | 0.258626 | 3:+19869167 | MsG0380012565.01.T01:CDS |
| ATCAAGGCACTTGTTGAATA+TGG | 0.262018 | 3:+19869721 | MsG0380012565.01.T01:CDS |
| ACTTGACATGTATGAGGTTT+TGG | 0.277794 | 3:+19869492 | MsG0380012565.01.T01:CDS |
| TTTGTTGCAAAGTTTGAGTT+AGG | 0.281815 | 3:-19868793 | None:intergenic |
| CGGCCTCACCAAAATTTAAA+AGG | 0.284169 | 3:-19869429 | None:intergenic |
| TCTTCGCGAAGTTCTTTATC+AGG | 0.287927 | 3:-19870204 | None:intergenic |
| ATGAGTTTGGAGACAAATAT+TGG | 0.301189 | 3:+19868722 | MsG0380012565.01.T01:CDS |
| CCAGGAAAACATTTATTTGA+TGG | 0.305526 | 3:-19869658 | None:intergenic |
| TGAGAATGGTGCAAAGATTT+TGG | 0.309875 | 3:-19870369 | None:intergenic |
| CAATTGTGTAAGCAGATTCT+TGG | 0.317014 | 3:+19869346 | MsG0380012565.01.T01:CDS |
| CACAAACTTCTAAGAAGCTT+TGG | 0.327792 | 3:+19869583 | MsG0380012565.01.T01:CDS |
| TGAAGATAAGGTAATGAGTT+TGG | 0.328772 | 3:+19868709 | MsG0380012565.01.T01:CDS |
| TTTGATTGATTGAACACAAT+TGG | 0.329865 | 3:-19868640 | None:intergenic |
| GCAAGATAAAGAAATGGATT+TGG | 0.330593 | 3:+19869281 | MsG0380012565.01.T01:CDS |
| GTTCAATTGCTTGCAACTTA+AGG | 0.341266 | 3:+19869833 | MsG0380012565.01.T01:CDS |
| GAACTTGTTCAAAGAGCTAA+TGG | 0.347236 | 3:+19868851 | MsG0380012565.01.T01:CDS |
| CAAAAGTTTGATTCTGTGAA+TGG | 0.352739 | 3:+19868812 | MsG0380012565.01.T01:CDS |
| ATACACAATATCAACTCTAT+TGG | 0.354741 | 3:-19868972 | None:intergenic |
| TCTTCACAAGCTGAGATCAT+AGG | 0.372051 | 3:-19868515 | None:intergenic |
| CAAGATAAAGAAATGGATTT+GGG | 0.378488 | 3:+19869282 | MsG0380012565.01.T01:CDS |
| TCGCGAAGATCTACAAATAT+CGG | 0.379075 | 3:+19870218 | MsG0380012565.01.T01:CDS |
| TCCTAGAACTGATGAAGATA+TGG | 0.393569 | 3:-19869168 | None:intergenic |
| AGCGAGCAGTGCTATAGATT+TGG | 0.401050 | 3:+19869069 | MsG0380012565.01.T01:CDS |
| ATTGTGTATGATGGATCATT+TGG | 0.401464 | 3:+19868986 | MsG0380012565.01.T01:CDS |
| CATTCATTACATGGTTCAAA+AGG | 0.407674 | 3:+19869945 | MsG0380012565.01.T01:CDS |
| ATCTATAGCACTGCTCGCTT+CGG | 0.413945 | 3:-19869065 | None:intergenic |
| CTTGGATTTGTTGCACAAGT+TGG | 0.424158 | 3:-19869878 | None:intergenic |
| TTCACTTTCTATGTTGAGAA+TGG | 0.431758 | 3:-19870383 | None:intergenic |
| CTCCAAAATATCTCGGAGTT+CGG | 0.439692 | 3:+19869399 | MsG0380012565.01.T01:CDS |
| TGCAGCTCAACACATTATAA+AGG | 0.458305 | 3:+19868556 | MsG0380012565.01.T01:CDS |
| GAATCAAAAGACATATAATC+AGG | 0.459827 | 3:-19868950 | None:intergenic |
| GCTTAAATGTGTTGAAGATA+AGG | 0.460156 | 3:+19868697 | MsG0380012565.01.T01:CDS |
| TGAGAAGTGGATTAAGTATA+CGG | 0.464454 | 3:+19870302 | MsG0380012565.01.T01:CDS |
| CAGTTCTAGGAGAGAAGCCT+TGG | 0.468878 | 3:+19869180 | MsG0380012565.01.T01:CDS |
| TGAATCGCAAGATAAAGAAA+TGG | 0.475005 | 3:+19869275 | MsG0380012565.01.T01:CDS |
| TTTGTTTCTCCAAAATATCT+CGG | 0.480530 | 3:+19869392 | MsG0380012565.01.T01:CDS |
| TTGTTTACTTGACATGTATG+AGG | 0.480736 | 3:+19869486 | MsG0380012565.01.T01:CDS |
| TTTGGTGAGGCCGTAACAAT+GGG | 0.483386 | 3:+19869439 | MsG0380012565.01.T01:CDS |
| GTTTGTTTCAAATTACCATC+AGG | 0.487592 | 3:+19869138 | MsG0380012565.01.T01:CDS |
| CCGGTATAACTTCGGTAAGC+AGG | 0.491544 | 3:-19870258 | None:intergenic |
| GTCAAGGCTTGAGGATGAGT+TGG | 0.492679 | 3:+19868892 | MsG0380012565.01.T01:CDS |
| ATCGTTCATACGTATCTAGT+TGG | 0.494827 | 3:+19869316 | MsG0380012565.01.T01:CDS |
| GAAGTATACAAAACACAGAC+AGG | 0.498418 | 3:+19870171 | MsG0380012565.01.T01:CDS |
| AGCAACATCAGTGTATAACT+TGG | 0.499600 | 3:-19869896 | None:intergenic |
| GAATGGAAATTTGAAGCATA+AGG | 0.507338 | 3:+19868829 | MsG0380012565.01.T01:CDS |
| CCTGCTTACCGAAGTTATAC+CGG | 0.507813 | 3:+19870258 | MsG0380012565.01.T01:CDS |
| TTGTTGCAAAGTTTGAGTTA+GGG | 0.513478 | 3:-19868792 | None:intergenic |
| CGTCTAACATTGATGAGAAG+TGG | 0.516919 | 3:+19870289 | MsG0380012565.01.T01:CDS |
| AGAGTTGATATTGTGTATGA+TGG | 0.520793 | 3:+19868977 | MsG0380012565.01.T01:CDS |
| GATGAATAACATTCATTACA+TGG | 0.527155 | 3:+19869936 | MsG0380012565.01.T01:CDS |
| AATCTCCAAAGAAATTACAT+AGG | 0.528067 | 3:-19869984 | None:intergenic |
| TCATGACATATCTTGTAAGA+TGG | 0.530309 | 3:-19869693 | None:intergenic |
| ATCTCCAAAGAAATTACATA+GGG | 0.535733 | 3:-19869983 | None:intergenic |
| TGGATCATTTGGTTCAATTG+AGG | 0.538106 | 3:+19868997 | MsG0380012565.01.T01:CDS |
| TTCTGGCTTCGCCAACATGT+TGG | 0.541758 | 3:+19870006 | MsG0380012565.01.T01:CDS |
| GTGTTCAATCAATCAAAGAA+AGG | 0.555579 | 3:+19868646 | MsG0380012565.01.T01:CDS |
| GAAGCTATGAGAAGGTGACT+TGG | 0.564885 | 3:+19870049 | MsG0380012565.01.T01:CDS |
| AATGTTAGACGAGTTTCTAC+CGG | 0.566162 | 3:-19870277 | None:intergenic |
| AAGCAGATTCTTGGAGACTT+CGG | 0.569927 | 3:+19869355 | MsG0380012565.01.T01:CDS |
| AGATATGGCAAAACTCCTGA+TGG | 0.585596 | 3:-19869153 | None:intergenic |
| AGTTGTTATGTCAAGGCTTG+AGG | 0.587807 | 3:+19868883 | MsG0380012565.01.T01:CDS |
| CGTGTGTGCTCCCATTGTTA+CGG | 0.593909 | 3:-19869449 | None:intergenic |
| GATGCGTCGTGGAGCAGTGA+TGG | 0.601043 | 3:+19869034 | MsG0380012565.01.T01:CDS |
| GCTGAAACATTCCAACATGT+TGG | 0.601281 | 3:-19870017 | None:intergenic |
| GAGGAAAGTTTATCGAATTG+CGG | 0.602785 | 3:+19870096 | MsG0380012565.01.T01:CDS |
| AGTACTTTCTGTGTTTAGTG+AGG | 0.603239 | 3:+19870077 | MsG0380012565.01.T01:CDS |
| AATGAGAAAGTACTCATCCA+AGG | 0.603868 | 3:-19869197 | None:intergenic |
| AGATGGTGAACACCTCCACC+AGG | 0.608901 | 3:-19869676 | None:intergenic |
| AAAATGTGCTTAAATTGGAG+TGG | 0.609715 | 3:+19869245 | MsG0380012565.01.T01:CDS |
| TGAAACTTCGACTGATCTTG+CGG | 0.612837 | 3:+19869771 | MsG0380012565.01.T01:CDS |
| AGTTTCTACCGGTATAACTT+CGG | 0.613307 | 3:-19870266 | None:intergenic |
| GCACAAGTTGGATTCTAGAG+TGG | 0.627118 | 3:-19869866 | None:intergenic |
| TTATGCTAGAAGCTATGAGA+AGG | 0.634466 | 3:+19870041 | MsG0380012565.01.T01:CDS |
| ATATGTCATGAATTACATCA+AGG | 0.636314 | 3:+19869705 | MsG0380012565.01.T01:CDS |
| TAGCACTGCTCGCTTCGGAT+CGG | 0.659065 | 3:-19869060 | None:intergenic |
| GCGTCGTGGAGCAGTGATGG+TGG | 0.670396 | 3:+19869037 | MsG0380012565.01.T01:CDS |
| TATACAAAACACAGACAGGG+TGG | 0.686135 | 3:+19870175 | MsG0380012565.01.T01:CDS |
| TTGTGAAGAAGATCAAGACA+TGG | 0.702934 | 3:+19868529 | MsG0380012565.01.T01:CDS |
| AAGTATACAAAACACAGACA+GGG | 0.732452 | 3:+19870172 | MsG0380012565.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTGAGTATTTAAAAATTGT+TGG | + | Chr3:19868758-19868777 | MsG0380012565.01.T01:CDS | 20.0% |
| !!! | AAGTAAACAAAACAGTTTTT+CGG | - | Chr3:19869476-19869495 | None:intergenic | 20.0% |
| !!! | TGATCTACAATGTTATATTT+TGG | + | Chr3:19870329-19870348 | MsG0380012565.01.T01:CDS | 20.0% |
| ! | AAAACCCTATGTAATTTCTT+TGG | + | Chr3:19869979-19869998 | MsG0380012565.01.T01:CDS | 25.0% |
| ! | AATCTCCAAAGAAATTACAT+AGG | - | Chr3:19869987-19870006 | None:intergenic | 25.0% |
| ! | ATACACAATATCAACTCTAT+TGG | - | Chr3:19868975-19868994 | None:intergenic | 25.0% |
| ! | ATATGTCATGAATTACATCA+AGG | + | Chr3:19869705-19869724 | MsG0380012565.01.T01:CDS | 25.0% |
| ! | ATCTCCAAAGAAATTACATA+GGG | - | Chr3:19869986-19870005 | None:intergenic | 25.0% |
| ! | GAATCAAAAGACATATAATC+AGG | - | Chr3:19868953-19868972 | None:intergenic | 25.0% |
| ! | GATGAATAACATTCATTACA+TGG | + | Chr3:19869936-19869955 | MsG0380012565.01.T01:CDS | 25.0% |
| ! | TTTGATTGATTGAACACAAT+TGG | - | Chr3:19868643-19868662 | None:intergenic | 25.0% |
| !! | AGTAAACAAAACAGTTTTTC+GGG | - | Chr3:19869475-19869494 | None:intergenic | 25.0% |
| !! | CAAGATAAAGAAATGGATTT+GGG | + | Chr3:19869282-19869301 | MsG0380012565.01.T01:CDS | 25.0% |
| !! | TTTGTTTCTCCAAAATATCT+CGG | + | Chr3:19869392-19869411 | MsG0380012565.01.T01:CDS | 25.0% |
| !!! | AAAGATTTTAGTACTGCTTT+TGG | + | Chr3:19870147-19870166 | MsG0380012565.01.T01:CDS | 25.0% |
| AAAATGTGCTTAAATTGGAG+TGG | + | Chr3:19869245-19869264 | MsG0380012565.01.T01:CDS | 30.0% | |
| AAGTATACAAAACACAGACA+GGG | + | Chr3:19870172-19870191 | MsG0380012565.01.T01:CDS | 30.0% | |
| ATGAGTTTGGAGACAAATAT+TGG | + | Chr3:19868722-19868741 | MsG0380012565.01.T01:CDS | 30.0% | |
| CAAAAGTTTGATTCTGTGAA+TGG | + | Chr3:19868812-19868831 | MsG0380012565.01.T01:CDS | 30.0% | |
| CATCGAAAATGTGCTTAAAT+TGG | + | Chr3:19869240-19869259 | MsG0380012565.01.T01:CDS | 30.0% | |
| CATCGACAATTAGAAGATTA+AGG | - | Chr3:19869753-19869772 | None:intergenic | 30.0% | |
| CATTCATTACATGGTTCAAA+AGG | + | Chr3:19869945-19869964 | MsG0380012565.01.T01:CDS | 30.0% | |
| CCAGGAAAACATTTATTTGA+TGG | - | Chr3:19869661-19869680 | None:intergenic | 30.0% | |
| GCTTAAATGTGTTGAAGATA+AGG | + | Chr3:19868697-19868716 | MsG0380012565.01.T01:CDS | 30.0% | |
| GTGTTCAATCAATCAAAGAA+AGG | + | Chr3:19868646-19868665 | MsG0380012565.01.T01:CDS | 30.0% | |
| GTTTGTTTCAAATTACCATC+AGG | + | Chr3:19869138-19869157 | MsG0380012565.01.T01:CDS | 30.0% | |
| TCATGACATATCTTGTAAGA+TGG | - | Chr3:19869696-19869715 | None:intergenic | 30.0% | |
| TGAAGATAAGGTAATGAGTT+TGG | + | Chr3:19868709-19868728 | MsG0380012565.01.T01:CDS | 30.0% | |
| TGAATCGCAAGATAAAGAAA+TGG | + | Chr3:19869275-19869294 | MsG0380012565.01.T01:CDS | 30.0% | |
| TTGTTTACTTGACATGTATG+AGG | + | Chr3:19869486-19869505 | MsG0380012565.01.T01:CDS | 30.0% | |
| ! | CCATCAAATAAATGTTTTCC+TGG | + | Chr3:19869658-19869677 | MsG0380012565.01.T01:CDS | 30.0% |
| ! | GAATGGAAATTTGAAGCATA+AGG | + | Chr3:19868829-19868848 | MsG0380012565.01.T01:CDS | 30.0% |
| ! | GCAAGATAAAGAAATGGATT+TGG | + | Chr3:19869281-19869300 | MsG0380012565.01.T01:CDS | 30.0% |
| ! | GTAATTTCTTTGGAGATTTC+TGG | + | Chr3:19869989-19870008 | MsG0380012565.01.T01:CDS | 30.0% |
| ! | GTATTGCGCCTTTTAAATTT+TGG | + | Chr3:19869421-19869440 | MsG0380012565.01.T01:CDS | 30.0% |
| ! | TCAAATAAATGTTTTCCTGG+TGG | + | Chr3:19869661-19869680 | MsG0380012565.01.T01:CDS | 30.0% |
| ! | TTGTTGCAAAGTTTGAGTTA+GGG | - | Chr3:19868795-19868814 | None:intergenic | 30.0% |
| ! | TTTGTTGCAAAGTTTGAGTT+AGG | - | Chr3:19868796-19868815 | None:intergenic | 30.0% |
| ! | TTTTGCAAGTTGTTATGTCA+AGG | + | Chr3:19868876-19868895 | MsG0380012565.01.T01:CDS | 30.0% |
| !! | AGAGTTGATATTGTGTATGA+TGG | + | Chr3:19868977-19868996 | MsG0380012565.01.T01:CDS | 30.0% |
| !! | ATTGTGTATGATGGATCATT+TGG | + | Chr3:19868986-19869005 | MsG0380012565.01.T01:CDS | 30.0% |
| !! | GAAGTTGATTCTTTTGTTAG+AGG | + | Chr3:19869553-19869572 | MsG0380012565.01.T01:CDS | 30.0% |
| !! | TGAGAAGTGGATTAAGTATA+CGG | + | Chr3:19870302-19870321 | MsG0380012565.01.T01:CDS | 30.0% |
| AATGTTAGACGAGTTTCTAC+CGG | - | Chr3:19870280-19870299 | None:intergenic | 35.0% | |
| AGCAACATCAGTGTATAACT+TGG | - | Chr3:19869899-19869918 | None:intergenic | 35.0% | |
| AGTACTTTCTGTGTTTAGTG+AGG | + | Chr3:19870077-19870096 | MsG0380012565.01.T01:CDS | 35.0% | |
| AGTTTCTACCGGTATAACTT+CGG | - | Chr3:19870269-19870288 | None:intergenic | 35.0% | |
| ATCGTTCATACGTATCTAGT+TGG | + | Chr3:19869316-19869335 | MsG0380012565.01.T01:CDS | 35.0% | |
| CAATTGTGTAAGCAGATTCT+TGG | + | Chr3:19869346-19869365 | MsG0380012565.01.T01:CDS | 35.0% | |
| GAAGTATACAAAACACAGAC+AGG | + | Chr3:19870171-19870190 | MsG0380012565.01.T01:CDS | 35.0% | |
| GAGGAAAGTTTATCGAATTG+CGG | + | Chr3:19870096-19870115 | MsG0380012565.01.T01:CDS | 35.0% | |
| GGAGAAACAAAGCTGATAAA+TGG | - | Chr3:19869383-19869402 | None:intergenic | 35.0% | |
| GTTCAATTGCTTGCAACTTA+AGG | + | Chr3:19869833-19869852 | MsG0380012565.01.T01:CDS | 35.0% | |
| TCCTAGAACTGATGAAGATA+TGG | - | Chr3:19869171-19869190 | None:intergenic | 35.0% | |
| TCGCGAAGATCTACAAATAT+CGG | + | Chr3:19870218-19870237 | MsG0380012565.01.T01:CDS | 35.0% | |
| TGCAGCTCAACACATTATAA+AGG | + | Chr3:19868556-19868575 | MsG0380012565.01.T01:CDS | 35.0% | |
| TTATGCTAGAAGCTATGAGA+AGG | + | Chr3:19870041-19870060 | MsG0380012565.01.T01:CDS | 35.0% | |
| TTGTGAAGAAGATCAAGACA+TGG | + | Chr3:19868529-19868548 | MsG0380012565.01.T01:CDS | 35.0% | |
| ! | AATAAATGTTTTCCTGGTGG+AGG | + | Chr3:19869664-19869683 | MsG0380012565.01.T01:CDS | 35.0% |
| ! | ACTTGACATGTATGAGGTTT+TGG | + | Chr3:19869492-19869511 | MsG0380012565.01.T01:CDS | 35.0% |
| ! | ATCAAGGCACTTGTTGAATA+TGG | + | Chr3:19869721-19869740 | MsG0380012565.01.T01:CDS | 35.0% |
| ! | CACAAACTTCTAAGAAGCTT+TGG | + | Chr3:19869583-19869602 | MsG0380012565.01.T01:CDS | 35.0% |
| ! | GAACTTGTTCAAAGAGCTAA+TGG | + | Chr3:19868851-19868870 | MsG0380012565.01.T01:CDS | 35.0% |
| ! | TGAGAATGGTGCAAAGATTT+TGG | - | Chr3:19870372-19870391 | None:intergenic | 35.0% |
| ! | TGAGTTGAGAAAAACTCTCT+TGG | + | Chr3:19868607-19868626 | MsG0380012565.01.T01:CDS | 35.0% |
| ! | TGGATCATTTGGTTCAATTG+AGG | + | Chr3:19868997-19869016 | MsG0380012565.01.T01:CDS | 35.0% |
| !! | AATGAGAAAGTACTCATCCA+AGG | - | Chr3:19869200-19869219 | None:intergenic | 35.0% |
| AAAAAATCAACGATGCGTCG+TGG | + | Chr3:19869023-19869042 | MsG0380012565.01.T01:CDS | 40.0% | |
| AAGCAGATTCTTGGAGACTT+CGG | + | Chr3:19869355-19869374 | MsG0380012565.01.T01:CDS | 40.0% | |
| AGATATGGCAAAACTCCTGA+TGG | - | Chr3:19869156-19869175 | None:intergenic | 40.0% | |
| AGTTGTTATGTCAAGGCTTG+AGG | + | Chr3:19868883-19868902 | MsG0380012565.01.T01:CDS | 40.0% | |
| CGGCCTCACCAAAATTTAAA+AGG | - | Chr3:19869432-19869451 | None:intergenic | 40.0% | |
| CGTCTAACATTGATGAGAAG+TGG | + | Chr3:19870289-19870308 | MsG0380012565.01.T01:CDS | 40.0% | |
| CTCCAAAATATCTCGGAGTT+CGG | + | Chr3:19869399-19869418 | MsG0380012565.01.T01:CDS | 40.0% | |
| GCCATATCTTCATCAGTTCT+AGG | + | Chr3:19869167-19869186 | MsG0380012565.01.T01:CDS | 40.0% | |
| GCTGAAACATTCCAACATGT+TGG | - | Chr3:19870020-19870039 | None:intergenic | 40.0% | |
| TATACAAAACACAGACAGGG+TGG | + | Chr3:19870175-19870194 | MsG0380012565.01.T01:CDS | 40.0% | |
| TCTTCGCGAAGTTCTTTATC+AGG | - | Chr3:19870207-19870226 | None:intergenic | 40.0% | |
| TGAAACTTCGACTGATCTTG+CGG | + | Chr3:19869771-19869790 | MsG0380012565.01.T01:CDS | 40.0% | |
| ! | TACCGAACTCCGAGATATTT+TGG | - | Chr3:19869404-19869423 | None:intergenic | 40.0% |
| !! | CTTGGATTTGTTGCACAAGT+TGG | - | Chr3:19869881-19869900 | None:intergenic | 40.0% |
| !! | GCGCCTTTTAAATTTTGGTG+AGG | + | Chr3:19869426-19869445 | MsG0380012565.01.T01:CDS | 40.0% |
| AGCGAGCAGTGCTATAGATT+TGG | + | Chr3:19869069-19869088 | MsG0380012565.01.T01:CDS | 45.0% | |
| ATCTATAGCACTGCTCGCTT+CGG | - | Chr3:19869068-19869087 | None:intergenic | 45.0% | |
| CCTGCTTACCGAAGTTATAC+CGG | + | Chr3:19870258-19870277 | MsG0380012565.01.T01:CDS | 45.0% | |
| GAAGCTATGAGAAGGTGACT+TGG | + | Chr3:19870049-19870068 | MsG0380012565.01.T01:CDS | 45.0% | |
| TTTGGTGAGGCCGTAACAAT+GGG | + | Chr3:19869439-19869458 | MsG0380012565.01.T01:CDS | 45.0% | |
| ! | GCACAAGTTGGATTCTAGAG+TGG | - | Chr3:19869869-19869888 | None:intergenic | 45.0% |
| ! | TTTTGGTGAGGCCGTAACAA+TGG | + | Chr3:19869438-19869457 | MsG0380012565.01.T01:CDS | 45.0% |
| !!! | GGTTTTGGAACTTCTAGCAG+TGG | + | Chr3:19869507-19869526 | MsG0380012565.01.T01:CDS | 45.0% |
| CCGGTATAACTTCGGTAAGC+AGG | - | Chr3:19870261-19870280 | None:intergenic | 50.0% | |
| CGTGTGTGCTCCCATTGTTA+CGG | - | Chr3:19869452-19869471 | None:intergenic | 50.0% | |
| GTCAAGGCTTGAGGATGAGT+TGG | + | Chr3:19868892-19868911 | MsG0380012565.01.T01:CDS | 50.0% | |
| !! | CAGTTCTAGGAGAGAAGCCT+TGG | + | Chr3:19869180-19869199 | MsG0380012565.01.T01:CDS | 50.0% |
| !! | TTCTGGCTTCGCCAACATGT+TGG | + | Chr3:19870006-19870025 | MsG0380012565.01.T01:CDS | 50.0% |
| AGATGGTGAACACCTCCACC+AGG | - | Chr3:19869679-19869698 | None:intergenic | 55.0% | |
| TAGCACTGCTCGCTTCGGAT+CGG | - | Chr3:19869063-19869082 | None:intergenic | 55.0% | |
| GATGCGTCGTGGAGCAGTGA+TGG | + | Chr3:19869034-19869053 | MsG0380012565.01.T01:CDS | 60.0% | |
| GCGTCGTGGAGCAGTGATGG+TGG | + | Chr3:19869037-19869056 | MsG0380012565.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 19868518 | 19870404 | 19868518 | ID=MsG0380012565.01;Name=MsG0380012565.01 |
| Chr3 | mRNA | 19868518 | 19870404 | 19868518 | ID=MsG0380012565.01.T01;Parent=MsG0380012565.01;Name=MsG0380012565.01.T01;_AED=0.34;_eAED=0.34;_QI=0|-1|0|1|-1|1|1|0|628 |
| Chr3 | exon | 19868518 | 19870404 | 19868518 | ID=MsG0380012565.01.T01:exon:7306;Parent=MsG0380012565.01.T01 |
| Chr3 | CDS | 19868518 | 19870404 | 19868518 | ID=MsG0380012565.01.T01:cds;Parent=MsG0380012565.01.T01 |
| Gene Sequence |
| Protein sequence |
