Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006762.01.T01 | XP_003593635.1 | 94.34 | 265 | 13 | 2 | 1 | 265 | 1 | 263 | 3.89E-106 | 318 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006762.01.T01 | Q01197 | 37.548 | 261 | 109 | 15 | 1 | 242 | 1 | 226 | 1.35E-18 | 85.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006762.01.T01 | G7ISF3 | 94.340 | 265 | 13 | 2 | 1 | 265 | 1 | 263 | 1.86e-106 | 318 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180006000.01 | MsG0280006762.01 | 0.828794 | 7.374773e-55 | 7.406012e-52 |
| MsG0280006487.01 | MsG0280006762.01 | 0.808427 | 3.113686e-50 | 1.779875e-47 |
| MsG0280006762.01 | MsG0280010650.01 | 0.837885 | 3.991896e-57 | 5.257374e-54 |
| MsG0280006762.01 | MsG0280010779.01 | 0.801734 | 7.829915e-49 | 3.767241e-46 |
| MsG0280006762.01 | MsG0280011341.01 | 0.801697 | 7.966253e-49 | 3.829269e-46 |
| MsG0280006762.01 | MsG0380012101.01 | 0.806307 | 8.764423e-50 | 4.741277e-47 |
| MsG0280006762.01 | MsG0380016140.01 | 0.850241 | 1.927929e-60 | 3.752566e-57 |
| MsG0280006762.01 | MsG0380016920.01 | 0.800963 | 1.126179e-48 | 5.314415e-46 |
| MsG0280006762.01 | MsG0480018128.01 | 0.804080 | 2.564147e-49 | 1.310022e-46 |
| MsG0280006762.01 | MsG0480019710.01 | 0.805699 | 1.176779e-49 | 6.268046e-47 |
| MsG0280006762.01 | MsG0480020402.01 | 0.800673 | 1.290475e-48 | 6.044887e-46 |
| MsG0280006762.01 | MsG0480020699.01 | 0.802811 | 4.699712e-49 | 2.324264e-46 |
| MsG0280006762.01 | MsG0480020847.01 | 0.839111 | 1.926748e-57 | 2.634047e-54 |
| MsG0280006762.01 | MsG0480020909.01 | 0.805888 | 1.073998e-49 | 5.748144e-47 |
| MsG0280006762.01 | MsG0480020965.01 | 0.843801 | 1.120290e-58 | 1.772822e-55 |
| MsG0280006762.01 | MsG0480021013.01 | 0.821655 | 3.598109e-53 | 2.945109e-50 |
| MsG0280006762.01 | MsG0480022045.01 | 0.801399 | 9.172696e-49 | 4.376026e-46 |
| MsG0280006762.01 | MsG0480023647.01 | 0.801738 | 7.816015e-49 | 3.760865e-46 |
| MsG0280006762.01 | MsG0480023671.01 | 0.815737 | 7.932574e-52 | 5.514458e-49 |
| MsG0280006762.01 | MsG0580024330.01 | 0.815716 | 8.016194e-52 | 5.569325e-49 |
| MsG0280006762.01 | MsG0580024489.01 | 0.808966 | 2.388298e-50 | 1.384645e-47 |
| MsG0280006762.01 | MsG0580025614.01 | 0.820002 | 8.632509e-53 | 6.748577e-50 |
| MsG0280006762.01 | MsG0580026089.01 | 0.804487 | 2.109938e-49 | 1.089237e-46 |
| MsG0280006762.01 | MsG0680030949.01 | 0.809829 | 1.559062e-50 | 9.249198e-48 |
| MsG0280006762.01 | MsG0780040307.01 | 0.811719 | 6.082795e-51 | 3.793311e-48 |
| MsG0280006762.01 | MsG0880042836.01 | 0.813295 | 2.751263e-51 | 1.789694e-48 |
| MsG0280006762.01 | MsG0880043516.01 | 0.811233 | 7.755721e-51 | 4.774993e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006762.01.T01 | MTR_2g014400 | 94.340 | 265 | 13 | 2 | 1 | 265 | 1 | 263 | 4.71e-110 | 318 |
| MsG0280006762.01.T01 | MTR_4g088385 | 56.618 | 136 | 47 | 5 | 131 | 262 | 230 | 357 | 7.79e-22 | 93.6 |
| MsG0280006762.01.T01 | MTR_4g088385 | 41.791 | 134 | 75 | 2 | 1 | 132 | 1 | 133 | 1.91e-18 | 84.0 |
| MsG0280006762.01.T01 | MTR_2g090660 | 30.469 | 256 | 118 | 8 | 21 | 255 | 21 | 237 | 4.32e-13 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006762.01.T01 | AT2G33850 | 32.710 | 214 | 94 | 11 | 21 | 203 | 22 | 216 | 7.49e-11 | 61.6 |
Find 49 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTCTGGTTTGTTTGTAAC+TGG | 0.131657 | 2:+5949051 | None:intergenic |
| TGTAGTTGCCATGGTTATTC+TGG | 0.221088 | 2:+5948482 | None:intergenic |
| TTGTGTCACTCAACTCATTT+TGG | 0.248728 | 2:+5948824 | None:intergenic |
| CAAAATAGCTATGGCTTATA+TGG | 0.251779 | 2:-5949003 | MsG0280006762.01.T01:CDS |
| TAGTACTTGTTATTGTTGTT+GGG | 0.319644 | 2:+5948901 | None:intergenic |
| GTAGTACTTGTTATTGTTGT+TGG | 0.324905 | 2:+5948900 | None:intergenic |
| ATGAAAGGTGGTTGCTCCTC+TGG | 0.328935 | 2:+5949036 | None:intergenic |
| TATTACAACAAAGACAGTTT+TGG | 0.329701 | 2:-5948853 | MsG0280006762.01.T01:CDS |
| AACTGGGGATTCTTCTTTGT+TGG | 0.334551 | 2:+5949068 | None:intergenic |
| ATTATAATGCTCAGAATAAC+AGG | 0.351179 | 2:-5948681 | MsG0280006762.01.T01:CDS |
| GGTGGATGCCGTGGCGGCGG+TGG | 0.353742 | 2:+5948957 | None:intergenic |
| ACTGGGGATTCTTCTTTGTT+GGG | 0.370013 | 2:+5949069 | None:intergenic |
| CCTCTGGTTTGTTTGTAACT+GGG | 0.383487 | 2:+5949052 | None:intergenic |
| TACAACAATAATAATAACTA+TGG | 0.387734 | 2:-5948520 | MsG0280006762.01.T01:CDS |
| GATGAATCACCATACATTGT+TGG | 0.414049 | 2:+5948571 | None:intergenic |
| TGTTTCTTCAAAGTTAGAAT+TGG | 0.416528 | 2:+5948933 | None:intergenic |
| GCTGAAGAATTCACTCTCTC+TGG | 0.421980 | 2:+5949125 | None:intergenic |
| GAGTGAACAACAATAATAAC+TGG | 0.430385 | 2:-5948543 | MsG0280006762.01.T01:CDS |
| TTCTTCAAAGTTAGAATTGG+TGG | 0.439756 | 2:+5948936 | None:intergenic |
| AAATACAATCCAACAATGTA+TGG | 0.452288 | 2:-5948580 | MsG0280006762.01.T01:CDS |
| TAAGAGGATGAAAGAAATGT+AGG | 0.456040 | 2:+5949179 | None:intergenic |
| GATGCCGTGGCGGCGGTGGA+AGG | 0.457928 | 2:+5948961 | None:intergenic |
| GGTGGTGGATGCCGTGGCGG+CGG | 0.460270 | 2:+5948954 | None:intergenic |
| CTCTGGTTTGTTTGTAACTG+GGG | 0.464914 | 2:+5949053 | None:intergenic |
| AATTATTATAATTATTGTTG+TGG | 0.469980 | 2:+5948701 | None:intergenic |
| TGTTGTTGAAGTAGTACTCT+TGG | 0.484835 | 2:+5948752 | None:intergenic |
| GCAACAAAAGAGTTGTTAAG+AGG | 0.489505 | 2:+5949163 | None:intergenic |
| AGGATGAAAGAAATGTAGGT+TGG | 0.497294 | 2:+5949183 | None:intergenic |
| GGATGAAAGAAATGTAGGTT+GGG | 0.498004 | 2:+5949184 | None:intergenic |
| TGTAACCATTGTAGTTGCCA+TGG | 0.515915 | 2:+5948473 | None:intergenic |
| TTCAAAGTTAGAATTGGTGG+TGG | 0.520386 | 2:+5948939 | None:intergenic |
| CCTCAAACACAAAATAGCTA+TGG | 0.533746 | 2:-5949012 | MsG0280006762.01.T01:CDS |
| AGTACTTGTTATTGTTGTTG+GGG | 0.535885 | 2:+5948902 | None:intergenic |
| TGTGTTTGAGGGATGAAAGG+TGG | 0.538392 | 2:+5949024 | None:intergenic |
| GACACAAAGTACAATGAAGA+AGG | 0.560331 | 2:-5948808 | MsG0280006762.01.T01:CDS |
| CATTCCTTCCACCGCCGCCA+CGG | 0.564976 | 2:-5948965 | MsG0280006762.01.T01:CDS |
| AGAAGGTTACAACTCCATGA+TGG | 0.580265 | 2:-5948791 | MsG0280006762.01.T01:CDS |
| AGGTACAATGGTGAGAAACA+AGG | 0.586625 | 2:-5948661 | MsG0280006762.01.T01:CDS |
| ATGTATGGTGATTCATCTAG+AGG | 0.598645 | 2:-5948565 | MsG0280006762.01.T01:CDS |
| AATAACCATGGCAACTACAA+TGG | 0.610902 | 2:-5948478 | MsG0280006762.01.T01:CDS |
| GCTCAGAATAACAGGTACAA+TGG | 0.619912 | 2:-5948673 | MsG0280006762.01.T01:CDS |
| AAGGAATGTGATCAGAGTCA+TGG | 0.626750 | 2:+5948980 | None:intergenic |
| AGAATTGGTGGTGGATGCCG+TGG | 0.633457 | 2:+5948948 | None:intergenic |
| ATTGGTGGTGGATGCCGTGG+CGG | 0.636866 | 2:+5948951 | None:intergenic |
| GGTACAATGGTGAGAAACAA+GGG | 0.647160 | 2:-5948660 | MsG0280006762.01.T01:CDS |
| AGAAATGTAGGTTGGGAATG+TGG | 0.649922 | 2:+5949191 | None:intergenic |
| TCTCTGGCTATAGTTTGTAG+AGG | 0.668021 | 2:+5949141 | None:intergenic |
| AATGAGTACCAGAATAACCA+TGG | 0.671041 | 2:-5948490 | MsG0280006762.01.T01:CDS |
| CCCAGTTACAAACAAACCAG+AGG | 0.679700 | 2:-5949052 | MsG0280006762.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTATTATAATTATTGTTG+TGG | + | Chr2:5948917-5948936 | None:intergenic | 10.0% |
| !! | TACAACAATAATAATAACTA+TGG | - | Chr2:5949095-5949114 | MsG0280006762.01.T01:CDS | 15.0% |
| ! | AAATACAATCCAACAATGTA+TGG | - | Chr2:5949035-5949054 | MsG0280006762.01.T01:CDS | 25.0% |
| ! | ATTATAATGCTCAGAATAAC+AGG | - | Chr2:5948934-5948953 | MsG0280006762.01.T01:CDS | 25.0% |
| ! | TGTTTCTTCAAAGTTAGAAT+TGG | + | Chr2:5948685-5948704 | None:intergenic | 25.0% |
| !! | TATTACAACAAAGACAGTTT+TGG | - | Chr2:5948762-5948781 | MsG0280006762.01.T01:CDS | 25.0% |
| !!! | TAGTACTTGTTATTGTTGTT+GGG | + | Chr2:5948717-5948736 | None:intergenic | 25.0% |
| CAAAATAGCTATGGCTTATA+TGG | - | Chr2:5948612-5948631 | MsG0280006762.01.T01:CDS | 30.0% | |
| GAGTGAACAACAATAATAAC+TGG | - | Chr2:5949072-5949091 | MsG0280006762.01.T01:CDS | 30.0% | |
| TAAGAGGATGAAAGAAATGT+AGG | + | Chr2:5948439-5948458 | None:intergenic | 30.0% | |
| TTCTTCAAAGTTAGAATTGG+TGG | + | Chr2:5948682-5948701 | None:intergenic | 30.0% | |
| !! | AGTACTTGTTATTGTTGTTG+GGG | + | Chr2:5948716-5948735 | None:intergenic | 30.0% |
| !! | CATAGCTATTTTGTGTTTGA+GGG | + | Chr2:5948605-5948624 | None:intergenic | 30.0% |
| !! | GTAGTACTTGTTATTGTTGT+TGG | + | Chr2:5948718-5948737 | None:intergenic | 30.0% |
| !!! | GTTGTTTTGTTTTTCCATCA+TGG | + | Chr2:5948841-5948860 | None:intergenic | 30.0% |
| AATAACCATGGCAACTACAA+TGG | - | Chr2:5949137-5949156 | MsG0280006762.01.T01:CDS | 35.0% | |
| AATGAGTACCAGAATAACCA+TGG | - | Chr2:5949125-5949144 | MsG0280006762.01.T01:CDS | 35.0% | |
| AGGATGAAAGAAATGTAGGT+TGG | + | Chr2:5948435-5948454 | None:intergenic | 35.0% | |
| ATGTATGGTGATTCATCTAG+AGG | - | Chr2:5949050-5949069 | MsG0280006762.01.T01:CDS | 35.0% | |
| CAATGGTTACAAAAACCAAG+AGG | - | Chr2:5949154-5949173 | MsG0280006762.01.T01:CDS | 35.0% | |
| CCTCAAACACAAAATAGCTA+TGG | - | Chr2:5948603-5948622 | MsG0280006762.01.T01:CDS | 35.0% | |
| GACACAAAGTACAATGAAGA+AGG | - | Chr2:5948807-5948826 | MsG0280006762.01.T01:CDS | 35.0% | |
| GATGAATCACCATACATTGT+TGG | + | Chr2:5949047-5949066 | None:intergenic | 35.0% | |
| GCAACAAAAGAGTTGTTAAG+AGG | + | Chr2:5948455-5948474 | None:intergenic | 35.0% | |
| GGATGAAAGAAATGTAGGTT+GGG | + | Chr2:5948434-5948453 | None:intergenic | 35.0% | |
| TGTTGTTGAAGTAGTACTCT+TGG | + | Chr2:5948866-5948885 | None:intergenic | 35.0% | |
| TTACAAAAACCAAGAGGAGT+TGG | - | Chr2:5949160-5949179 | MsG0280006762.01.T01:CDS | 35.0% | |
| TTCAAAGTTAGAATTGGTGG+TGG | + | Chr2:5948679-5948698 | None:intergenic | 35.0% | |
| ! | GATGAGTGACACAAGATTTT+TGG | - | Chr2:5948977-5948996 | MsG0280006762.01.T01:CDS | 35.0% |
| ! | TTTTGTGTTTGAGGGATGAA+AGG | + | Chr2:5948597-5948616 | None:intergenic | 35.0% |
| !! | AGTGACACAAGATTTTTGGA+AGG | - | Chr2:5948981-5949000 | MsG0280006762.01.T01:CDS | 35.0% |
| !! | CCATAGCTATTTTGTGTTTG+AGG | + | Chr2:5948606-5948625 | None:intergenic | 35.0% |
| !! | TTGTGTCACTCAACTCATTT+TGG | + | Chr2:5948794-5948813 | None:intergenic | 35.0% |
| AACTGGGGATTCTTCTTTGT+TGG | + | Chr2:5948550-5948569 | None:intergenic | 40.0% | |
| AAGGAATGTGATCAGAGTCA+TGG | + | Chr2:5948638-5948657 | None:intergenic | 40.0% | |
| ACTGGGGATTCTTCTTTGTT+GGG | + | Chr2:5948549-5948568 | None:intergenic | 40.0% | |
| AGAAGGTTACAACTCCATGA+TGG | - | Chr2:5948824-5948843 | MsG0280006762.01.T01:CDS | 40.0% | |
| AGGTACAATGGTGAGAAACA+AGG | - | Chr2:5948954-5948973 | MsG0280006762.01.T01:CDS | 40.0% | |
| GCTCAGAATAACAGGTACAA+TGG | - | Chr2:5948942-5948961 | MsG0280006762.01.T01:CDS | 40.0% | |
| GGTACAATGGTGAGAAACAA+GGG | - | Chr2:5948955-5948974 | MsG0280006762.01.T01:CDS | 40.0% | |
| TGTAACCATTGTAGTTGCCA+TGG | + | Chr2:5949145-5949164 | None:intergenic | 40.0% | |
| TGTAGTTGCCATGGTTATTC+TGG | + | Chr2:5949136-5949155 | None:intergenic | 40.0% | |
| ! | AGAAATGTAGGTTGGGAATG+TGG | + | Chr2:5948427-5948446 | None:intergenic | 40.0% |
| ! | GTTCATTTTCCAACTCCTCT+TGG | + | Chr2:5949172-5949191 | None:intergenic | 40.0% |
| ! | TCTCTGGCTATAGTTTGTAG+AGG | + | Chr2:5948477-5948496 | None:intergenic | 40.0% |
| !! | CCTCTGGTTTGTTTGTAACT+GGG | + | Chr2:5948566-5948585 | None:intergenic | 40.0% |
| !! | CTCTGGTTTGTTTGTAACTG+GGG | + | Chr2:5948565-5948584 | None:intergenic | 40.0% |
| !! | GACACAAGATTTTTGGAAGG+TGG | - | Chr2:5948984-5949003 | MsG0280006762.01.T01:CDS | 40.0% |
| !! | TCCTCTGGTTTGTTTGTAAC+TGG | + | Chr2:5948567-5948586 | None:intergenic | 40.0% |
| CCCAGTTACAAACAAACCAG+AGG | - | Chr2:5948563-5948582 | MsG0280006762.01.T01:CDS | 45.0% | |
| GCTGAAGAATTCACTCTCTC+TGG | + | Chr2:5948493-5948512 | None:intergenic | 45.0% | |
| TGTGTTTGAGGGATGAAAGG+TGG | + | Chr2:5948594-5948613 | None:intergenic | 45.0% | |
| ATGAAAGGTGGTTGCTCCTC+TGG | + | Chr2:5948582-5948601 | None:intergenic | 50.0% | |
| !! | AGAATTGGTGGTGGATGCCG+TGG | + | Chr2:5948670-5948689 | None:intergenic | 55.0% |
| !! | ATTGGTGGTGGATGCCGTGG+CGG | + | Chr2:5948667-5948686 | None:intergenic | 60.0% |
| CATTCCTTCCACCGCCGCCA+CGG | - | Chr2:5948650-5948669 | MsG0280006762.01.T01:CDS | 65.0% | |
| GATGCCGTGGCGGCGGTGGA+AGG | + | Chr2:5948657-5948676 | None:intergenic | 75.0% | |
| !! | GGTGGTGGATGCCGTGGCGG+CGG | + | Chr2:5948664-5948683 | None:intergenic | 75.0% |
| ! | GGTGGATGCCGTGGCGGCGG+TGG | + | Chr2:5948661-5948680 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 5948420 | 5949217 | 5948420 | ID=MsG0280006762.01;Name=MsG0280006762.01 |
| Chr2 | mRNA | 5948420 | 5949217 | 5948420 | ID=MsG0280006762.01.T01;Parent=MsG0280006762.01;Name=MsG0280006762.01.T01;_AED=0.27;_eAED=0.27;_QI=0|-1|0|1|-1|1|1|0|265 |
| Chr2 | exon | 5948420 | 5949217 | 5948420 | ID=MsG0280006762.01.T01:exon:2405;Parent=MsG0280006762.01.T01 |
| Chr2 | CDS | 5948420 | 5949217 | 5948420 | ID=MsG0280006762.01.T01:cds;Parent=MsG0280006762.01.T01 |
| Gene Sequence |
| Protein sequence |
