Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024330.01.T01 | GAU12459.1 | 57.102 | 352 | 65 | 6 | 1 | 266 | 59 | 410 | 5.54E-110 | 338 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024330.01.T01 | Q9ZPY9 | 56 | 150 | 51 | 1 | 132 | 266 | 282 | 431 | 8.38E-45 | 162 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024330.01.T01 | A0A2Z6LGF6 | 57.102 | 352 | 65 | 6 | 1 | 266 | 59 | 410 | 2.65e-110 | 338 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047974.01 | MsG0580024330.01 | 0.805906 | 1.064524e-49 | 5.700089e-47 |
| MsG0080048029.01 | MsG0580024330.01 | 0.802469 | 5.527333e-49 | 2.709750e-46 |
| MsG0080048291.01 | MsG0580024330.01 | 0.802267 | 6.085222e-49 | 2.967880e-46 |
| MsG0080048856.01 | MsG0580024330.01 | 0.800561 | 1.360121e-48 | 6.352902e-46 |
| MsG0080049140.01 | MsG0580024330.01 | 0.841615 | 4.268184e-58 | 6.306504e-55 |
| MsG0180000142.01 | MsG0580024330.01 | 0.809109 | 2.225691e-50 | 1.295398e-47 |
| MsG0180000324.01 | MsG0580024330.01 | 0.837472 | 5.094783e-57 | 6.625210e-54 |
| MsG0180000348.01 | MsG0580024330.01 | 0.815342 | 9.711930e-52 | 6.678323e-49 |
| MsG0180000557.01 | MsG0580024330.01 | 0.815020 | 1.144741e-51 | 7.802822e-49 |
| MsG0180001173.01 | MsG0580024330.01 | 0.801377 | 9.266443e-49 | 4.418502e-46 |
| MsG0180002421.01 | MsG0580024330.01 | 0.804251 | 2.362831e-49 | 1.212375e-46 |
| MsG0180003361.01 | MsG0580024330.01 | 0.805473 | 1.312680e-49 | 6.950858e-47 |
| MsG0180003920.01 | MsG0580024330.01 | 0.830412 | 2.979930e-55 | 3.137236e-52 |
| MsG0180003943.01 | MsG0580024330.01 | 0.806314 | 8.738218e-50 | 4.727804e-47 |
| MsG0180004099.01 | MsG0580024330.01 | 0.834733 | 2.526131e-56 | 3.024001e-53 |
| MsG0180004754.01 | MsG0580024330.01 | 0.849649 | 2.823440e-60 | 5.389161e-57 |
| MsG0180004958.01 | MsG0580024330.01 | 0.839270 | 1.752166e-57 | 2.407135e-54 |
| MsG0180005021.01 | MsG0580024330.01 | 0.807464 | 4.990404e-50 | 2.781547e-47 |
| MsG0180005024.01 | MsG0580024330.01 | 0.811856 | 5.678667e-51 | 3.554137e-48 |
| MsG0180005025.01 | MsG0580024330.01 | 0.820718 | 5.916163e-53 | 4.717461e-50 |
| MsG0180006000.01 | MsG0580024330.01 | 0.831512 | 1.600835e-55 | 1.741039e-52 |
| MsG0280010650.01 | MsG0580024330.01 | 0.823619 | 1.256638e-53 | 1.087588e-50 |
| MsG0280010779.01 | MsG0580024330.01 | 0.829547 | 4.842457e-55 | 4.971602e-52 |
| MsG0280010907.01 | MsG0580024330.01 | 0.803179 | 3.944651e-49 | 1.969142e-46 |
| MsG0280010977.01 | MsG0580024330.01 | 0.809012 | 2.334826e-50 | 1.355282e-47 |
| MsG0280011014.01 | MsG0580024330.01 | 0.839662 | 1.385945e-57 | 1.927014e-54 |
| MsG0280011034.01 | MsG0580024330.01 | 0.847447 | 1.150088e-59 | 2.044799e-56 |
| MsG0280011154.01 | MsG0580024330.01 | 0.839397 | 1.623505e-57 | 2.239372e-54 |
| MsG0280011258.01 | MsG0580024330.01 | 0.824215 | 9.108203e-54 | 8.016383e-51 |
| MsG0280011282.01 | MsG0580024330.01 | 0.814487 | 1.502741e-51 | 1.009690e-48 |
| MsG0380012471.01 | MsG0580024330.01 | 0.800458 | 1.427601e-48 | 6.650560e-46 |
| MsG0380013475.01 | MsG0580024330.01 | 0.806717 | 7.182770e-50 | 3.926518e-47 |
| MsG0480018918.01 | MsG0580024330.01 | 0.800136 | 1.660378e-48 | 7.672156e-46 |
| MsG0480019350.01 | MsG0580024330.01 | 0.815876 | 7.384688e-52 | 5.152649e-49 |
| MsG0480019710.01 | MsG0580024330.01 | 0.828837 | 7.199966e-55 | 7.239201e-52 |
| MsG0480019922.01 | MsG0580024330.01 | 0.829648 | 4.575727e-55 | 4.712211e-52 |
| MsG0480020495.01 | MsG0580024330.01 | 0.834399 | 3.064714e-56 | 3.632852e-53 |
| MsG0480020847.01 | MsG0580024330.01 | 0.816425 | 5.566987e-52 | 3.943132e-49 |
| MsG0480020965.01 | MsG0580024330.01 | 0.802664 | 5.038553e-49 | 2.482321e-46 |
| MsG0480021013.01 | MsG0580024330.01 | 0.802949 | 4.400321e-49 | 2.183693e-46 |
| MsG0480022045.01 | MsG0580024330.01 | 0.803185 | 3.933664e-49 | 1.963936e-46 |
| MsG0480022325.01 | MsG0580024330.01 | 0.832857 | 7.439849e-56 | 8.420288e-53 |
| MsG0480022549.01 | MsG0580024330.01 | 0.804102 | 2.537382e-49 | 1.297056e-46 |
| MsG0480022620.01 | MsG0580024330.01 | 0.826284 | 2.951808e-54 | 2.756169e-51 |
| MsG0480023262.01 | MsG0580024330.01 | 0.802348 | 5.853770e-49 | 2.861029e-46 |
| MsG0480023296.01 | MsG0580024330.01 | 0.810310 | 1.228199e-50 | 7.378267e-48 |
| MsG0480023463.01 | MsG0580024330.01 | 0.806752 | 7.062235e-50 | 3.864112e-47 |
| MsG0480023500.01 | MsG0580024330.01 | 0.806395 | 8.397926e-50 | 4.553067e-47 |
| MsG0480023620.01 | MsG0580024330.01 | 0.834460 | 2.959429e-56 | 3.514588e-53 |
| MsG0480023647.01 | MsG0580024330.01 | 0.803169 | 3.963696e-49 | 1.978157e-46 |
| MsG0580024330.01 | MsG0580024489.01 | 0.872964 | 2.121106e-67 | 9.046849e-64 |
| MsG0580024330.01 | MsG0580025352.01 | 0.801885 | 7.288866e-49 | 3.520721e-46 |
| MsG0580024330.01 | MsG0580025614.01 | 0.848873 | 4.643776e-60 | 8.643706e-57 |
| MsG0580024330.01 | MsG0580026059.01 | 0.824866 | 6.400312e-54 | 5.739764e-51 |
| MsG0580024330.01 | MsG0580026089.01 | 0.806657 | 7.396079e-50 | 4.036646e-47 |
| MsG0580024330.01 | MsG0580026593.01 | 0.836535 | 8.842041e-57 | 1.117462e-53 |
| MsG0580024330.01 | MsG0580026610.01 | 0.823044 | 1.712335e-53 | 1.458158e-50 |
| MsG0580024330.01 | MsG0580027650.01 | 0.800627 | 1.318752e-48 | 6.170087e-46 |
| MsG0580024330.01 | MsG0580028060.01 | 0.812195 | 4.789861e-51 | 3.025711e-48 |
| MsG0580024330.01 | MsG0580028176.01 | 0.827107 | 1.878269e-54 | 1.795741e-51 |
| MsG0580024330.01 | MsG0580028436.01 | 0.814269 | 1.679123e-51 | 1.121546e-48 |
| MsG0580024330.01 | MsG0580028756.01 | 0.817864 | 2.642700e-52 | 1.947194e-49 |
| MsG0580024330.01 | MsG0580029640.01 | 0.839057 | 1.989785e-57 | 2.715828e-54 |
| MsG0580024330.01 | MsG0580029825.01 | 0.801808 | 7.561108e-49 | 3.644838e-46 |
| MsG0580024330.01 | MsG0680030949.01 | 0.826942 | 2.056022e-54 | 1.956401e-51 |
| MsG0580024330.01 | MsG0680034842.01 | 0.800048 | 1.730121e-48 | 7.976493e-46 |
| MsG0580024330.01 | MsG0680034853.01 | 0.812872 | 3.406401e-51 | 2.190630e-48 |
| MsG0580024330.01 | MsG0780036147.01 | 0.804152 | 2.477234e-49 | 1.267942e-46 |
| MsG0580024330.01 | MsG0780036477.01 | 0.811623 | 6.380629e-51 | 3.968865e-48 |
| MsG0580024330.01 | MsG0780036752.01 | 0.852829 | 3.571336e-61 | 7.559680e-58 |
| MsG0580024330.01 | MsG0780036815.01 | 0.807380 | 5.199060e-50 | 2.891494e-47 |
| MsG0580024330.01 | MsG0780038424.01 | 0.836686 | 8.092974e-57 | 1.027505e-53 |
| MsG0580024330.01 | MsG0780039425.01 | 0.824066 | 9.871992e-54 | 8.651943e-51 |
| MsG0580024330.01 | MsG0780039655.01 | 0.801336 | 9.447008e-49 | 4.499999e-46 |
| MsG0580024330.01 | MsG0780039712.01 | 0.807664 | 4.525782e-50 | 2.535563e-47 |
| MsG0580024330.01 | MsG0780040023.01 | 0.802430 | 5.632473e-49 | 2.758576e-46 |
| MsG0580024330.01 | MsG0780040307.01 | 0.802442 | 5.598282e-49 | 2.742650e-46 |
| MsG0580024330.01 | MsG0780041063.01 | 0.815993 | 6.952201e-52 | 4.866689e-49 |
| MsG0580024330.01 | MsG0780041535.01 | 0.821063 | 4.926780e-53 | 3.967027e-50 |
| MsG0580024330.01 | MsG0880042773.01 | 0.802931 | 4.438539e-49 | 2.201661e-46 |
| MsG0580024330.01 | MsG0880042836.01 | 0.814429 | 1.547628e-51 | 1.038212e-48 |
| MsG0580024330.01 | MsG0880042961.01 | 0.839997 | 1.133868e-57 | 1.593371e-54 |
| MsG0580024330.01 | MsG0880042964.01 | 0.802526 | 5.380395e-49 | 2.641418e-46 |
| MsG0580024330.01 | MsG0880043034.01 | 0.811482 | 6.848564e-51 | 4.244352e-48 |
| MsG0580024330.01 | MsG0880043516.01 | 0.837495 | 5.026114e-57 | 6.540780e-54 |
| MsG0580024330.01 | MsG0880044782.01 | 0.804588 | 2.010445e-49 | 1.040585e-46 |
| MsG0580024330.01 | MsG0880045506.01 | 0.818021 | 2.435405e-52 | 1.802146e-49 |
| MsG0580024330.01 | MsG0880045552.01 | 0.814983 | 1.166911e-51 | 7.945780e-49 |
| MsG0580024330.01 | MsG0880047226.01 | 0.851837 | 6.842238e-61 | 1.402621e-57 |
| MsG0580024330.01 | MsG0880047666.01 | 0.814643 | 1.388017e-51 | 9.365273e-49 |
| MsG0380015548.01 | MsG0580024330.01 | 0.829586 | 4.739494e-55 | 4.871675e-52 |
| MsG0380015944.01 | MsG0580024330.01 | 0.813313 | 2.726497e-51 | 1.774476e-48 |
| MsG0380016140.01 | MsG0580024330.01 | 0.824442 | 8.054609e-54 | 7.134902e-51 |
| MsG0380016247.01 | MsG0580024330.01 | 0.812393 | 4.336013e-51 | 2.753750e-48 |
| MsG0380016703.01 | MsG0580024330.01 | 0.825186 | 5.375759e-54 | 4.865338e-51 |
| MsG0380016994.01 | MsG0580024330.01 | 0.855644 | 5.493807e-62 | 1.277148e-58 |
| MsG0380017159.01 | MsG0580024330.01 | 0.837251 | 5.803830e-57 | 7.495077e-54 |
| MsG0380017869.01 | MsG0580024330.01 | 0.829178 | 5.951222e-55 | 6.045037e-52 |
| MsG0480018230.01 | MsG0580024330.01 | 0.836610 | 8.459698e-57 | 1.071504e-53 |
| MsG0480018313.01 | MsG0580024330.01 | 0.837305 | 5.620424e-57 | 7.270698e-54 |
| MsG0280006477.01 | MsG0580024330.01 | 0.804748 | 1.861582e-49 | 9.676152e-47 |
| MsG0280006762.01 | MsG0580024330.01 | 0.815716 | 8.016194e-52 | 5.569325e-49 |
| MsG0280007452.01 | MsG0580024330.01 | 0.818797 | 1.625005e-52 | 1.228450e-49 |
| MsG0280008280.01 | MsG0580024330.01 | 0.804773 | 1.838701e-49 | 9.563050e-47 |
| MsG0280008614.01 | MsG0580024330.01 | 0.815897 | 7.304281e-52 | 5.099424e-49 |
| MsG0280009514.01 | MsG0580024330.01 | 0.805237 | 1.470464e-49 | 7.739534e-47 |
| MsG0280009752.01 | MsG0580024330.01 | 0.809221 | 2.106486e-50 | 1.229666e-47 |
| MsG0280009794.01 | MsG0580024330.01 | 0.824415 | 8.173923e-54 | 7.234767e-51 |
| MsG0280010491.01 | MsG0580024330.01 | 0.829682 | 4.489354e-55 | 4.627967e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024330.01.T01 | MTR_5g009120 | 97.345 | 113 | 3 | 0 | 1 | 113 | 57 | 169 | 2.64e-71 | 229 |
| MsG0580024330.01.T01 | MTR_5g009120 | 67.333 | 150 | 27 | 2 | 122 | 249 | 282 | 431 | 6.73e-54 | 184 |
| MsG0580024330.01.T01 | MTR_8g099565 | 57.432 | 148 | 43 | 1 | 122 | 249 | 295 | 442 | 1.88e-47 | 167 |
| MsG0580024330.01.T01 | MTR_8g099565 | 61.947 | 113 | 41 | 1 | 1 | 113 | 53 | 163 | 5.82e-38 | 140 |
| MsG0580024330.01.T01 | MTR_8g027610 | 57.447 | 141 | 45 | 2 | 132 | 257 | 308 | 448 | 1.47e-43 | 156 |
| MsG0580024330.01.T01 | MTR_8g027610 | 50.442 | 113 | 53 | 2 | 1 | 113 | 64 | 173 | 1.11e-27 | 112 |
| MsG0580024330.01.T01 | MTR_7g117395 | 57.931 | 145 | 36 | 4 | 125 | 249 | 301 | 440 | 1.58e-42 | 153 |
| MsG0580024330.01.T01 | MTR_7g117395 | 51.485 | 101 | 47 | 1 | 13 | 113 | 77 | 175 | 4.46e-27 | 110 |
| MsG0580024330.01.T01 | MTR_3g091400 | 56.115 | 139 | 40 | 2 | 132 | 249 | 289 | 427 | 1.35e-40 | 148 |
| MsG0580024330.01.T01 | MTR_3g091400 | 46.018 | 113 | 59 | 1 | 1 | 113 | 53 | 163 | 4.54e-25 | 104 |
| MsG0580024330.01.T01 | MTR_8g066090 | 40.972 | 144 | 47 | 4 | 132 | 249 | 187 | 318 | 9.83e-22 | 95.1 |
| MsG0580024330.01.T01 | MTR_0009s0120 | 38.667 | 150 | 54 | 4 | 126 | 249 | 183 | 320 | 7.08e-21 | 92.4 |
| MsG0580024330.01.T01 | MTR_7g017360 | 41.667 | 132 | 63 | 4 | 132 | 249 | 185 | 316 | 8.42e-21 | 92.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024330.01.T01 | AT2G46500 | 56.000 | 150 | 51 | 1 | 132 | 266 | 282 | 431 | 8.54e-46 | 162 |
| MsG0580024330.01.T01 | AT2G46500 | 38.406 | 138 | 83 | 1 | 1 | 138 | 61 | 196 | 7.88e-25 | 103 |
| MsG0580024330.01.T01 | AT2G46500 | 56.000 | 150 | 51 | 1 | 132 | 266 | 282 | 431 | 8.54e-46 | 162 |
| MsG0580024330.01.T01 | AT2G46500 | 38.406 | 138 | 83 | 1 | 1 | 138 | 61 | 196 | 7.88e-25 | 103 |
| MsG0580024330.01.T01 | AT5G24240 | 36.842 | 304 | 128 | 8 | 3 | 249 | 125 | 421 | 2.82e-41 | 150 |
| MsG0580024330.01.T01 | AT5G24240 | 38.235 | 136 | 82 | 1 | 1 | 136 | 59 | 192 | 2.58e-22 | 96.7 |
| MsG0580024330.01.T01 | AT5G24240 | 36.842 | 304 | 128 | 8 | 3 | 249 | 125 | 421 | 2.82e-41 | 150 |
| MsG0580024330.01.T01 | AT5G24240 | 38.235 | 136 | 82 | 1 | 1 | 136 | 59 | 192 | 2.58e-22 | 96.7 |
| MsG0580024330.01.T01 | AT2G46500 | 53.448 | 116 | 39 | 1 | 132 | 232 | 282 | 397 | 5.30e-30 | 117 |
| MsG0580024330.01.T01 | AT2G46500 | 38.406 | 138 | 83 | 1 | 1 | 138 | 61 | 196 | 4.44e-25 | 103 |
| MsG0580024330.01.T01 | AT3G56600 | 40.000 | 140 | 54 | 4 | 128 | 249 | 123 | 250 | 2.11e-22 | 96.7 |
| MsG0580024330.01.T01 | AT3G56600 | 40.000 | 140 | 54 | 4 | 128 | 249 | 126 | 253 | 2.15e-22 | 96.7 |
| MsG0580024330.01.T01 | AT3G56600 | 40.000 | 140 | 54 | 4 | 128 | 249 | 126 | 253 | 2.15e-22 | 96.7 |
| MsG0580024330.01.T01 | AT2G03890 | 39.333 | 150 | 53 | 4 | 132 | 255 | 192 | 329 | 5.35e-22 | 95.9 |
| MsG0580024330.01.T01 | AT1G26270 | 39.333 | 150 | 53 | 4 | 132 | 255 | 188 | 325 | 1.75e-21 | 94.4 |
| MsG0580024330.01.T01 | AT1G13640 | 38.158 | 152 | 54 | 4 | 132 | 255 | 184 | 323 | 6.84e-21 | 92.4 |
| MsG0580024330.01.T01 | AT1G13640 | 38.158 | 152 | 54 | 4 | 132 | 255 | 184 | 323 | 6.84e-21 | 92.4 |
| MsG0580024330.01.T01 | AT2G40850 | 37.410 | 139 | 54 | 3 | 132 | 249 | 147 | 273 | 3.07e-20 | 90.5 |
| MsG0580024330.01.T01 | AT2G03890 | 35.772 | 123 | 42 | 3 | 158 | 255 | 99 | 209 | 3.24e-13 | 69.7 |
Find 65 sgRNAs with CRISPR-Local
Find 108 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGAAGTTGCGGCTTATATT+TGG | 0.277975 | 5:+3484750 | MsG0580024330.01.T01:CDS |
| TAATAATCCACGTGGATTGC+CGG | 0.330333 | 5:+3484670 | MsG0580024330.01.T01:CDS |
| TCCACGTGGATTGCCGGTTT+CGG | 0.331568 | 5:+3484676 | MsG0580024330.01.T01:CDS |
| TAATGGTAATGTTGTTCATT+TGG | 0.353220 | 5:+3484039 | MsG0580024330.01.T01:CDS |
| ACCAAACGCGGTCCAGCCTT+TGG | 0.378332 | 5:-3484778 | None:intergenic |
| CCCTCACCATTCACCGAAAC+CGG | 0.379422 | 5:-3484689 | None:intergenic |
| GCCATTGGTTCCTCATCAAT+TGG | 0.383037 | 5:-3484644 | None:intergenic |
| TCCAAAGGCTGGACCGCGTT+TGG | 0.385239 | 5:+3484777 | MsG0580024330.01.T01:CDS |
| GATGGTAATTGTGAGGATAT+TGG | 0.395024 | 5:+3484945 | MsG0580024330.01.T01:CDS |
| GCTCTTTGAGGATGGATCTA+AGG | 0.400574 | 5:+3484893 | MsG0580024330.01.T01:intron |
| TTGAAGAGAGGGACAAAGGT+TGG | 0.402804 | 5:+3484713 | MsG0580024330.01.T01:CDS |
| CAGAAGTTTATGAGTAATGA+TGG | 0.410311 | 5:+3484927 | MsG0580024330.01.T01:CDS |
| TAGGAATTGTATTAAAAGAA+AGG | 0.420136 | 5:+3484159 | MsG0580024330.01.T01:CDS |
| AAATGGTGACTTACTTTCTC+AGG | 0.421776 | 5:-3485118 | None:intergenic |
| GCTGGACCGCGTTTGGTGTC+TGG | 0.423380 | 5:+3484784 | MsG0580024330.01.T01:CDS |
| AGGAATTGTATTAAAAGAAA+GGG | 0.424400 | 5:+3484160 | MsG0580024330.01.T01:CDS |
| GAATCAGGATGAGGTTGTTA+AGG | 0.426840 | 5:+3484413 | MsG0580024330.01.T01:CDS |
| ATAGGAACGTTGCACATCTT+AGG | 0.430624 | 5:+3484140 | MsG0580024330.01.T01:CDS |
| AAGACAATAACCATGATCAA+TGG | 0.441498 | 5:-3485095 | None:intergenic |
| GCGAGATAATTCTCTTCCAT+TGG | 0.447190 | 5:-3483984 | None:intergenic |
| GAATTTGAATAATCAGAATC+AGG | 0.447450 | 5:+3484398 | MsG0580024330.01.T01:CDS |
| GGATTGCCGGTTTCGGTGAA+TGG | 0.453627 | 5:+3484683 | MsG0580024330.01.T01:CDS |
| CTGCTCATTCCCATTGATCA+TGG | 0.456539 | 5:+3485085 | MsG0580024330.01.T01:CDS |
| CCGGTTTCGGTGAATGGTGA+GGG | 0.469852 | 5:+3484689 | MsG0580024330.01.T01:CDS |
| AAAGAGTACGGTGTAACTAA+TGG | 0.470997 | 5:+3484022 | MsG0580024330.01.T01:CDS |
| AGACAATAACCATGATCAAT+GGG | 0.483074 | 5:-3485094 | None:intergenic |
| TATTTGGATCATCCAAAGGC+TGG | 0.483963 | 5:+3484766 | MsG0580024330.01.T01:CDS |
| TTTGAGGATGGATCTAAGGT+GGG | 0.490344 | 5:+3484897 | MsG0580024330.01.T01:intron |
| CGTGGATTATTAACCGCCAT+TGG | 0.491031 | 5:-3484659 | None:intergenic |
| AATTTGAGTTTCAGATTGAT+CGG | 0.502943 | 5:+3484116 | MsG0580024330.01.T01:CDS |
| GCGAAAAGCTTGATGATAAG+AGG | 0.505944 | 5:+3484233 | MsG0580024330.01.T01:CDS |
| GGGATTGAAGAGAGGGACAA+AGG | 0.509599 | 5:+3484709 | MsG0580024330.01.T01:CDS |
| AAGCAAAATTTAGTTTCCAA+TGG | 0.511426 | 5:+3483968 | MsG0580024330.01.T01:CDS |
| TTGTTTAGAAAAGAGTCAAA+TGG | 0.514272 | 5:+3485055 | MsG0580024330.01.T01:CDS |
| ATGGTGAGGGATTGAAGAGA+GGG | 0.516819 | 5:+3484702 | MsG0580024330.01.T01:CDS |
| TAAGAATGGCGAATGCAGAT+AGG | 0.518671 | 5:+3485018 | MsG0580024330.01.T01:CDS |
| GCCGGTTTCGGTGAATGGTG+AGG | 0.524007 | 5:+3484688 | MsG0580024330.01.T01:CDS |
| CTTTGAGGATGGATCTAAGG+TGG | 0.524289 | 5:+3484896 | MsG0580024330.01.T01:intron |
| ATCTTATGCACCTCTTGCAC+AGG | 0.541451 | 5:-3484981 | None:intergenic |
| TGTATTAAAAGAAAGGGGAA+AGG | 0.545961 | 5:+3484166 | MsG0580024330.01.T01:CDS |
| TTGGTGTCTGGTGAAGCGAT+TGG | 0.546477 | 5:+3484796 | MsG0580024330.01.T01:CDS |
| AGTTTCAGATTGATCGGCAT+AGG | 0.546957 | 5:+3484122 | MsG0580024330.01.T01:CDS |
| GGAATTGTATTAAAAGAAAG+GGG | 0.547344 | 5:+3484161 | MsG0580024330.01.T01:CDS |
| AGGAGCTGTGAGAGAAGTTG+CGG | 0.549114 | 5:+3484739 | MsG0580024330.01.T01:CDS |
| CAGTGTGCTTGATATAAGAA+TGG | 0.555819 | 5:+3485004 | MsG0580024330.01.T01:CDS |
| AGAGGGACAAAGGTTGGAGA+AGG | 0.563732 | 5:+3484719 | MsG0580024330.01.T01:CDS |
| GAATAATCAGAATCAGGATG+AGG | 0.566712 | 5:+3484404 | MsG0580024330.01.T01:CDS |
| AATGGTGAGGGATTGAAGAG+AGG | 0.566744 | 5:+3484701 | MsG0580024330.01.T01:CDS |
| GCGAATGCAGATAGGCATGC+AGG | 0.571832 | 5:+3485026 | MsG0580024330.01.T01:CDS |
| GCCAATTGATGAGGAACCAA+TGG | 0.574717 | 5:+3484643 | MsG0580024330.01.T01:intron |
| CTTATATTTGGATCATCCAA+AGG | 0.578232 | 5:+3484762 | MsG0580024330.01.T01:CDS |
| AATGTGGCGATGAAAGAGTA+CGG | 0.637828 | 5:+3484010 | MsG0580024330.01.T01:CDS |
| TTGAGGATGGATCTAAGGTG+GGG | 0.638841 | 5:+3484898 | MsG0580024330.01.T01:intron |
| AAACCGCACCATACCGTAGG+CGG | 0.639987 | 5:-3484833 | None:intergenic |
| TCTTGCACAGGAAAAGCACC+AGG | 0.657475 | 5:-3484969 | None:intergenic |
| AATTGATGAGGAACCAATGG+CGG | 0.662251 | 5:+3484646 | MsG0580024330.01.T01:CDS |
| TGTGAGGACAATGTGTGGGA+AGG | 0.662882 | 5:+3484093 | MsG0580024330.01.T01:CDS |
| ATTGTTGTGAGGACAATGTG+TGG | 0.666886 | 5:+3484088 | MsG0580024330.01.T01:CDS |
| TTGTTGTGAGGACAATGTGT+GGG | 0.668421 | 5:+3484089 | MsG0580024330.01.T01:CDS |
| AATTGTGAGGATATTGGACC+TGG | 0.674707 | 5:+3484951 | MsG0580024330.01.T01:CDS |
| AGAATTATCTCGCAACAATG+TGG | 0.674854 | 5:+3483994 | MsG0580024330.01.T01:CDS |
| ACCGAAACCGGCAATCCACG+TGG | 0.678913 | 5:-3484677 | None:intergenic |
| GAGTAATGATGGTAATTGTG+AGG | 0.714535 | 5:+3484938 | MsG0580024330.01.T01:CDS |
| ATGGCGGTTAATAATCCACG+TGG | 0.754648 | 5:+3484662 | MsG0580024330.01.T01:CDS |
| GCTTCACCAGACACCAAACG+CGG | 0.759039 | 5:-3484790 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCATCTTATTGTTAAAAAAT+CGG | + | Chr5:3484293-3484312 | MsG0580024330.01.T01:intron | 15.0% |
| !! | AGGAATTGTATTAAAAGAAA+GGG | + | Chr5:3484160-3484179 | MsG0580024330.01.T01:CDS | 20.0% |
| !! | TAGGAATTGTATTAAAAGAA+AGG | + | Chr5:3484159-3484178 | MsG0580024330.01.T01:CDS | 20.0% |
| ! | AAAGGAAAAAAATTGATCCT+AGG | - | Chr5:3484492-3484511 | None:intergenic | 25.0% |
| ! | AACAAAAAATCAAAACCAGA+GGG | - | Chr5:3484450-3484469 | None:intergenic | 25.0% |
| ! | AAGGAAAAAAATTGATCCTA+GGG | - | Chr5:3484491-3484510 | None:intergenic | 25.0% |
| ! | ACTTTCAATGGTTTGAAAAA+AGG | + | Chr5:3484534-3484553 | MsG0580024330.01.T01:intron | 25.0% |
| ! | GAATTTGAATAATCAGAATC+AGG | + | Chr5:3484398-3484417 | MsG0580024330.01.T01:CDS | 25.0% |
| ! | GGAATTGTATTAAAAGAAAG+GGG | + | Chr5:3484161-3484180 | MsG0580024330.01.T01:CDS | 25.0% |
| ! | TAACAAAAAATCAAAACCAG+AGG | - | Chr5:3484451-3484470 | None:intergenic | 25.0% |
| ! | TTGTTTAGAAAAGAGTCAAA+TGG | + | Chr5:3485055-3485074 | MsG0580024330.01.T01:CDS | 25.0% |
| !! | AAGCAAAATTTAGTTTCCAA+TGG | + | Chr5:3483968-3483987 | MsG0580024330.01.T01:CDS | 25.0% |
| !! | AATTTGAGTTTCAGATTGAT+CGG | + | Chr5:3484116-3484135 | MsG0580024330.01.T01:CDS | 25.0% |
| !! | ATATGATTTTCATTGTTGTG+AGG | + | Chr5:3484077-3484096 | MsG0580024330.01.T01:CDS | 25.0% |
| !! | TAATGGTAATGTTGTTCATT+TGG | + | Chr5:3484039-3484058 | MsG0580024330.01.T01:CDS | 25.0% |
| !!! | CAATTTTTTTCCTTTCCTAT+GGG | + | Chr5:3484497-3484516 | MsG0580024330.01.T01:intron | 25.0% |
| !!! | TCAATTTTTTTCCTTTCCTA+TGG | + | Chr5:3484496-3484515 | MsG0580024330.01.T01:intron | 25.0% |
| !!! | TGCTACTTTTGTTCATAATA+AGG | + | Chr5:3484326-3484345 | MsG0580024330.01.T01:intron | 25.0% |
| AAACACGCAAAAATGTTTCA+TGG | + | Chr5:3483942-3483961 | MsG0580024330.01.T01:CDS | 30.0% | |
| AAGACAATAACCATGATCAA+TGG | - | Chr5:3485098-3485117 | None:intergenic | 30.0% | |
| AGACAATAACCATGATCAAT+GGG | - | Chr5:3485097-3485116 | None:intergenic | 30.0% | |
| ATGATAGATGCAACTTTCAA+TGG | + | Chr5:3484522-3484541 | MsG0580024330.01.T01:intron | 30.0% | |
| CAGAAGTTTATGAGTAATGA+TGG | + | Chr5:3484927-3484946 | MsG0580024330.01.T01:CDS | 30.0% | |
| CTTATATTTGGATCATCCAA+AGG | + | Chr5:3484762-3484781 | MsG0580024330.01.T01:CDS | 30.0% | |
| TACTTCATGCAAGATTTCAA+AGG | + | Chr5:3484597-3484616 | MsG0580024330.01.T01:intron | 30.0% | |
| TGTATTAAAAGAAAGGGGAA+AGG | + | Chr5:3484166-3484185 | MsG0580024330.01.T01:CDS | 30.0% | |
| AAACCTGTTAGATCTTCTGA+GGG | + | Chr5:3484561-3484580 | MsG0580024330.01.T01:intron | 35.0% | |
| AAGCGTTTAATTACCCTGAT+GGG | + | Chr5:3484861-3484880 | MsG0580024330.01.T01:intron | 35.0% | |
| AGAATTATCTCGCAACAATG+TGG | + | Chr5:3483994-3484013 | MsG0580024330.01.T01:CDS | 35.0% | |
| CAGTGTGCTTGATATAAGAA+TGG | + | Chr5:3485004-3485023 | MsG0580024330.01.T01:CDS | 35.0% | |
| GAATAATCAGAATCAGGATG+AGG | + | Chr5:3484404-3484423 | MsG0580024330.01.T01:CDS | 35.0% | |
| GAGTAATGATGGTAATTGTG+AGG | + | Chr5:3484938-3484957 | MsG0580024330.01.T01:CDS | 35.0% | |
| GATGGTAATTGTGAGGATAT+TGG | + | Chr5:3484945-3484964 | MsG0580024330.01.T01:CDS | 35.0% | |
| TAAACCTGTTAGATCTTCTG+AGG | + | Chr5:3484560-3484579 | MsG0580024330.01.T01:intron | 35.0% | |
| TCTATCATATCCCATAGGAA+AGG | - | Chr5:3484510-3484529 | None:intergenic | 35.0% | |
| TTGCATCTATCATATCCCAT+AGG | - | Chr5:3484515-3484534 | None:intergenic | 35.0% | |
| ! | AAAGAGTACGGTGTAACTAA+TGG | + | Chr5:3484022-3484041 | MsG0580024330.01.T01:CDS | 35.0% |
| !! | CTGTTTTAAGCCAATTGATG+AGG | + | Chr5:3484634-3484653 | MsG0580024330.01.T01:intron | 35.0% |
| !!! | AGGTTTTGTTGATCTTGTTG+AGG | + | Chr5:3484186-3484205 | MsG0580024330.01.T01:CDS | 35.0% |
| !!! | GAACAAGAGTTTTTTTGCAG+CGG | + | Chr5:3484211-3484230 | MsG0580024330.01.T01:CDS | 35.0% |
| AAGGTTATAGAACAACCCTC+TGG | + | Chr5:3484432-3484451 | MsG0580024330.01.T01:intron | 40.0% | |
| AATGTGGCGATGAAAGAGTA+CGG | + | Chr5:3484010-3484029 | MsG0580024330.01.T01:CDS | 40.0% | |
| AATTGATGAGGAACCAATGG+CGG | + | Chr5:3484646-3484665 | MsG0580024330.01.T01:CDS | 40.0% | |
| AATTGTGAGGATATTGGACC+TGG | + | Chr5:3484951-3484970 | MsG0580024330.01.T01:CDS | 40.0% | |
| AGCGTTTAATTACCCTGATG+GGG | + | Chr5:3484862-3484881 | MsG0580024330.01.T01:intron | 40.0% | |
| AGTTTCAGATTGATCGGCAT+AGG | + | Chr5:3484122-3484141 | MsG0580024330.01.T01:CDS | 40.0% | |
| ATAGGAACGTTGCACATCTT+AGG | + | Chr5:3484140-3484159 | MsG0580024330.01.T01:CDS | 40.0% | |
| ATCCTAGGGTTCACGAAAAT+CGG | - | Chr5:3484477-3484496 | None:intergenic | 40.0% | |
| ATTGTTGTGAGGACAATGTG+TGG | + | Chr5:3484088-3484107 | MsG0580024330.01.T01:CDS | 40.0% | |
| CATGCAAGATTTCAAAGGTG+TGG | + | Chr5:3484602-3484621 | MsG0580024330.01.T01:intron | 40.0% | |
| GAATCAGGATGAGGTTGTTA+AGG | + | Chr5:3484413-3484432 | MsG0580024330.01.T01:CDS | 40.0% | |
| GAGAAGTTGCGGCTTATATT+TGG | + | Chr5:3484750-3484769 | MsG0580024330.01.T01:CDS | 40.0% | |
| GCGAGATAATTCTCTTCCAT+TGG | - | Chr5:3483987-3484006 | None:intergenic | 40.0% | |
| TAAGAATGGCGAATGCAGAT+AGG | + | Chr5:3485018-3485037 | MsG0580024330.01.T01:CDS | 40.0% | |
| TATTTGGATCATCCAAAGGC+TGG | + | Chr5:3484766-3484785 | MsG0580024330.01.T01:CDS | 40.0% | |
| TTGTTGTGAGGACAATGTGT+GGG | + | Chr5:3484089-3484108 | MsG0580024330.01.T01:CDS | 40.0% | |
| ! | GAAGCGTTTAATTACCCTGA+TGG | + | Chr5:3484860-3484879 | MsG0580024330.01.T01:intron | 40.0% |
| ! | GCGAAAAGCTTGATGATAAG+AGG | + | Chr5:3484233-3484252 | MsG0580024330.01.T01:CDS | 40.0% |
| ! | TAATAATCCACGTGGATTGC+CGG | + | Chr5:3484670-3484689 | MsG0580024330.01.T01:CDS | 40.0% |
| ! | TTTGAGGATGGATCTAAGGT+GGG | + | Chr5:3484897-3484916 | MsG0580024330.01.T01:intron | 40.0% |
| AATGGTGAGGGATTGAAGAG+AGG | + | Chr5:3484701-3484720 | MsG0580024330.01.T01:CDS | 45.0% | |
| ATCTTATGCACCTCTTGCAC+AGG | - | Chr5:3484984-3485003 | None:intergenic | 45.0% | |
| ATGGCGGTTAATAATCCACG+TGG | + | Chr5:3484662-3484681 | MsG0580024330.01.T01:CDS | 45.0% | |
| ATGGTGAGGGATTGAAGAGA+GGG | + | Chr5:3484702-3484721 | MsG0580024330.01.T01:CDS | 45.0% | |
| CGTTTAATTACCCTGATGGG+GGG | + | Chr5:3484864-3484883 | MsG0580024330.01.T01:intron | 45.0% | |
| CTGCTCATTCCCATTGATCA+TGG | + | Chr5:3485085-3485104 | MsG0580024330.01.T01:CDS | 45.0% | |
| GCCAATTGATGAGGAACCAA+TGG | + | Chr5:3484643-3484662 | MsG0580024330.01.T01:intron | 45.0% | |
| GCGTTTAATTACCCTGATGG+GGG | + | Chr5:3484863-3484882 | MsG0580024330.01.T01:intron | 45.0% | |
| GTTCCCTCAGAAGATCTAAC+AGG | - | Chr5:3484567-3484586 | None:intergenic | 45.0% | |
| TTGAAGAGAGGGACAAAGGT+TGG | + | Chr5:3484713-3484732 | MsG0580024330.01.T01:CDS | 45.0% | |
| ! | CGTGGATTATTAACCGCCAT+TGG | - | Chr5:3484662-3484681 | None:intergenic | 45.0% |
| ! | CTTTGAGGATGGATCTAAGG+TGG | + | Chr5:3484896-3484915 | MsG0580024330.01.T01:intron | 45.0% |
| ! | GCTCTTTGAGGATGGATCTA+AGG | + | Chr5:3484893-3484912 | MsG0580024330.01.T01:intron | 45.0% |
| ! | GTTAGATCTTCTGAGGGAAC+AGG | + | Chr5:3484567-3484586 | MsG0580024330.01.T01:intron | 45.0% |
| ! | TTGAGGATGGATCTAAGGTG+GGG | + | Chr5:3484898-3484917 | MsG0580024330.01.T01:intron | 45.0% |
| !! | AACCGATTTTCGTGAACCCT+AGG | + | Chr5:3484472-3484491 | MsG0580024330.01.T01:intron | 45.0% |
| !! | GCCATTGGTTCCTCATCAAT+TGG | - | Chr5:3484647-3484666 | None:intergenic | 45.0% |
| !!! | GTGAAGCGATTGGTTTTTGC+TGG | + | Chr5:3484806-3484825 | MsG0580024330.01.T01:CDS | 45.0% |
| AGAGGGACAAAGGTTGGAGA+AGG | + | Chr5:3484719-3484738 | MsG0580024330.01.T01:CDS | 50.0% | |
| AGGAGCTGTGAGAGAAGTTG+CGG | + | Chr5:3484739-3484758 | MsG0580024330.01.T01:CDS | 50.0% | |
| GGGATTGAAGAGAGGGACAA+AGG | + | Chr5:3484709-3484728 | MsG0580024330.01.T01:CDS | 50.0% | |
| TGTAAACCGCACCATACCGT+AGG | - | Chr5:3484839-3484858 | None:intergenic | 50.0% | |
| TGTGAGGACAATGTGTGGGA+AGG | + | Chr5:3484093-3484112 | MsG0580024330.01.T01:CDS | 50.0% | |
| ! | AGATCTTCTGAGGGAACAGG+TGG | + | Chr5:3484570-3484589 | MsG0580024330.01.T01:intron | 50.0% |
| ! | GATCTTCTGAGGGAACAGGT+GGG | + | Chr5:3484571-3484590 | MsG0580024330.01.T01:intron | 50.0% |
| ! | GGATTGTGGCTCTTTGAGGA+TGG | + | Chr5:3484885-3484904 | MsG0580024330.01.T01:intron | 50.0% |
| ! | TGGTGCTTTTCCTGTGCAAG+AGG | + | Chr5:3484971-3484990 | MsG0580024330.01.T01:CDS | 50.0% |
| ! | TTGGTGTCTGGTGAAGCGAT+TGG | + | Chr5:3484796-3484815 | MsG0580024330.01.T01:CDS | 50.0% |
| ! | TTTTGCTGGTGTTCCGCCTA+CGG | + | Chr5:3484820-3484839 | MsG0580024330.01.T01:CDS | 50.0% |
| !! | TCTTGCACAGGAAAAGCACC+AGG | - | Chr5:3484972-3484991 | None:intergenic | 50.0% |
| AAACCGCACCATACCGTAGG+CGG | - | Chr5:3484836-3484855 | None:intergenic | 55.0% | |
| CCCTCACCATTCACCGAAAC+CGG | - | Chr5:3484692-3484711 | None:intergenic | 55.0% | |
| CCGGTTTCGGTGAATGGTGA+GGG | + | Chr5:3484689-3484708 | MsG0580024330.01.T01:CDS | 55.0% | |
| GCGAATGCAGATAGGCATGC+AGG | + | Chr5:3485026-3485045 | MsG0580024330.01.T01:CDS | 55.0% | |
| GCTTCACCAGACACCAAACG+CGG | - | Chr5:3484793-3484812 | None:intergenic | 55.0% | |
| TTACCCTGATGGGGGGATTG+TGG | + | Chr5:3484871-3484890 | MsG0580024330.01.T01:intron | 55.0% | |
| !! | GGATTGCCGGTTTCGGTGAA+TGG | + | Chr5:3484683-3484702 | MsG0580024330.01.T01:CDS | 55.0% |
| !! | TCCACGTGGATTGCCGGTTT+CGG | + | Chr5:3484676-3484695 | MsG0580024330.01.T01:CDS | 55.0% |
| ACCAAACGCGGTCCAGCCTT+TGG | - | Chr5:3484781-3484800 | None:intergenic | 60.0% | |
| ACCGAAACCGGCAATCCACG+TGG | - | Chr5:3484680-3484699 | None:intergenic | 60.0% | |
| AGAGCCACAATCCCCCCATC+AGG | - | Chr5:3484878-3484897 | None:intergenic | 60.0% | |
| CTGGTGTTCCGCCTACGGTA+TGG | + | Chr5:3484825-3484844 | MsG0580024330.01.T01:intron | 60.0% | |
| GAGCCACAATCCCCCCATCA+GGG | - | Chr5:3484877-3484896 | None:intergenic | 60.0% | |
| GGGGGGATTGTGGCTCTTTG+AGG | + | Chr5:3484881-3484900 | MsG0580024330.01.T01:intron | 60.0% | |
| GTTCCGCCTACGGTATGGTG+CGG | + | Chr5:3484830-3484849 | MsG0580024330.01.T01:intron | 60.0% | |
| TCCAAAGGCTGGACCGCGTT+TGG | + | Chr5:3484777-3484796 | MsG0580024330.01.T01:CDS | 60.0% | |
| ! | GCCGGTTTCGGTGAATGGTG+AGG | + | Chr5:3484688-3484707 | MsG0580024330.01.T01:CDS | 60.0% |
| !! | GCTGGACCGCGTTTGGTGTC+TGG | + | Chr5:3484784-3484803 | MsG0580024330.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 3483932 | 3485130 | 3483932 | ID=MsG0580024330.01;Name=MsG0580024330.01 |
| Chr5 | mRNA | 3483932 | 3485130 | 3483932 | ID=MsG0580024330.01.T01;Parent=MsG0580024330.01;Name=MsG0580024330.01.T01;_AED=0.42;_eAED=0.42;_QI=0|0|0|1|0.66|0.75|4|0|275 |
| Chr5 | exon | 3483932 | 3484271 | 3483932 | ID=MsG0580024330.01.T01:exon:1097;Parent=MsG0580024330.01.T01 |
| Chr5 | exon | 3484373 | 3484434 | 3484373 | ID=MsG0580024330.01.T01:exon:1098;Parent=MsG0580024330.01.T01 |
| Chr5 | exon | 3484644 | 3484841 | 3484644 | ID=MsG0580024330.01.T01:exon:1099;Parent=MsG0580024330.01.T01 |
| Chr5 | exon | 3484903 | 3485130 | 3484903 | ID=MsG0580024330.01.T01:exon:1100;Parent=MsG0580024330.01.T01 |
| Chr5 | CDS | 3483932 | 3484271 | 3483932 | ID=MsG0580024330.01.T01:cds;Parent=MsG0580024330.01.T01 |
| Chr5 | CDS | 3484373 | 3484434 | 3484373 | ID=MsG0580024330.01.T01:cds;Parent=MsG0580024330.01.T01 |
| Chr5 | CDS | 3484644 | 3484841 | 3484644 | ID=MsG0580024330.01.T01:cds;Parent=MsG0580024330.01.T01 |
| Chr5 | CDS | 3484903 | 3485130 | 3484903 | ID=MsG0580024330.01.T01:cds;Parent=MsG0580024330.01.T01 |
| Gene Sequence |
| Protein sequence |
