Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006781.01.T01 | XP_003593663.1 | 97.917 | 96 | 2 | 0 | 1 | 96 | 32 | 127 | 1.42E-62 | 196 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006781.01.T01 | Q9SGP6 | 64 | 100 | 32 | 1 | 1 | 96 | 38 | 137 | 1.73E-40 | 133 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006781.01.T01 | A2Q531 | 97.917 | 96 | 2 | 0 | 1 | 96 | 32 | 127 | 6.78e-63 | 196 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048177.01 | MsG0280006781.01 | 0.817060 | 4.010947e-52 | 2.890961e-49 |
| MsG0080048178.01 | MsG0280006781.01 | 0.806771 | 6.995521e-50 | 3.829505e-47 |
| MsG0180001271.01 | MsG0280006781.01 | 0.809987 | 1.442141e-50 | 8.591255e-48 |
| MsG0180003241.01 | MsG0280006781.01 | 0.810830 | 9.481954e-51 | 5.775898e-48 |
| MsG0280006586.01 | MsG0280006781.01 | 0.830402 | 2.996304e-55 | 3.153540e-52 |
| MsG0280006781.01 | MsG0280007481.01 | 0.808661 | 2.774969e-50 | 1.595858e-47 |
| MsG0280006781.01 | MsG0280008220.01 | 0.811358 | 7.287284e-51 | 4.501072e-48 |
| MsG0280006781.01 | MsG0380014163.01 | 0.828857 | 7.121944e-55 | 7.164801e-52 |
| MsG0280006781.01 | MsG0380014680.01 | 0.806239 | 9.058591e-50 | 4.892128e-47 |
| MsG0280006781.01 | MsG0380015877.01 | 0.804410 | 2.189201e-49 | 1.127922e-46 |
| MsG0280006781.01 | MsG0380015986.01 | 0.817711 | 2.861630e-52 | 2.099671e-49 |
| MsG0280006781.01 | MsG0380017940.01 | 0.821507 | 3.891155e-53 | 3.171669e-50 |
| MsG0280006781.01 | MsG0480022661.01 | 0.815424 | 9.311675e-52 | 6.417443e-49 |
| MsG0280006781.01 | MsG0480023898.01 | 0.809239 | 2.087927e-50 | 1.219445e-47 |
| MsG0280006781.01 | MsG0580024205.01 | 0.808485 | 3.026297e-50 | 1.732528e-47 |
| MsG0280006781.01 | MsG0580024826.01 | 0.828154 | 1.052516e-54 | 1.037580e-51 |
| MsG0280006781.01 | MsG0580024856.01 | 0.801876 | 7.320486e-49 | 3.535180e-46 |
| MsG0280006781.01 | MsG0580027408.01 | 0.800838 | 1.194339e-48 | 5.617988e-46 |
| MsG0280006781.01 | MsG0580028980.01 | 0.809140 | 2.192061e-50 | 1.276869e-47 |
| MsG0280006781.01 | MsG0580029906.01 | 0.803368 | 3.605192e-49 | 1.808338e-46 |
| MsG0280006781.01 | MsG0680035460.01 | 0.819239 | 1.288970e-52 | 9.864399e-50 |
| MsG0280006781.01 | MsG0780040516.01 | 0.812269 | 4.616191e-51 | 2.921807e-48 |
| MsG0280006781.01 | MsG0780040679.01 | 0.840481 | 8.471642e-58 | 1.208298e-54 |
| MsG0280006781.01 | MsG0780041485.01 | 0.825488 | 4.562321e-54 | 4.164283e-51 |
| MsG0280006781.01 | MsG0780041777.01 | 0.839974 | 1.149535e-57 | 1.614199e-54 |
| MsG0280006781.01 | MsG0880042843.01 | 0.805157 | 1.528296e-49 | 8.027483e-47 |
| MsG0280006781.01 | MsG0880043279.01 | 0.816895 | 4.366985e-52 | 3.133314e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006781.01.T01 | MTR_2g014760 | 97.917 | 96 | 2 | 0 | 1 | 96 | 32 | 127 | 1.72e-66 | 196 |
| MsG0280006781.01.T01 | MTR_2g090755 | 68.041 | 97 | 30 | 1 | 1 | 96 | 44 | 140 | 8.46e-44 | 139 |
| MsG0280006781.01.T01 | MTR_4g079110 | 68.041 | 97 | 30 | 1 | 1 | 96 | 59 | 155 | 7.01e-43 | 137 |
| MsG0280006781.01.T01 | MTR_7g026770 | 52.525 | 99 | 42 | 2 | 1 | 96 | 28 | 124 | 1.16e-28 | 104 |
| MsG0280006781.01.T01 | MTR_7g022690 | 48.485 | 99 | 42 | 2 | 3 | 96 | 38 | 132 | 8.45e-28 | 98.2 |
| MsG0280006781.01.T01 | MTR_7g022550 | 47.368 | 95 | 49 | 1 | 3 | 96 | 38 | 132 | 1.74e-27 | 97.4 |
| MsG0280006781.01.T01 | MTR_7g022710 | 46.465 | 99 | 44 | 2 | 3 | 96 | 38 | 132 | 5.40e-27 | 96.3 |
| MsG0280006781.01.T01 | MTR_4g119050 | 43.564 | 101 | 48 | 2 | 1 | 96 | 7 | 103 | 1.74e-24 | 89.0 |
| MsG0280006781.01.T01 | MTR_4g119030 | 43.564 | 101 | 48 | 2 | 1 | 96 | 7 | 103 | 1.74e-24 | 89.0 |
| MsG0280006781.01.T01 | MTR_4g119040 | 43.564 | 101 | 48 | 2 | 1 | 96 | 7 | 103 | 1.74e-24 | 89.0 |
| MsG0280006781.01.T01 | MTR_2g019900 | 41.584 | 101 | 49 | 3 | 1 | 96 | 7 | 102 | 1.20e-22 | 84.3 |
| MsG0280006781.01.T01 | MTR_2g048970 | 41.000 | 100 | 55 | 1 | 1 | 96 | 34 | 133 | 2.93e-22 | 84.3 |
| MsG0280006781.01.T01 | MTR_1g088905 | 37.500 | 96 | 60 | 0 | 1 | 96 | 7 | 102 | 3.76e-21 | 80.5 |
| MsG0280006781.01.T01 | MTR_7g108250 | 40.196 | 102 | 49 | 2 | 1 | 96 | 7 | 102 | 4.15e-21 | 80.5 |
| MsG0280006781.01.T01 | MTR_1g015890 | 44.444 | 99 | 51 | 2 | 1 | 96 | 52 | 149 | 5.55e-21 | 81.6 |
| MsG0280006781.01.T01 | MTR_3g104510 | 42.708 | 96 | 49 | 2 | 1 | 95 | 27 | 117 | 8.52e-20 | 77.4 |
| MsG0280006781.01.T01 | MTR_1g088920 | 40.449 | 89 | 53 | 0 | 1 | 89 | 7 | 95 | 1.96e-19 | 76.3 |
| MsG0280006781.01.T01 | MTR_5g077550 | 50.000 | 84 | 40 | 2 | 15 | 96 | 40 | 123 | 2.36e-19 | 76.6 |
| MsG0280006781.01.T01 | MTR_2g019950 | 36.458 | 96 | 61 | 0 | 1 | 96 | 7 | 102 | 3.21e-19 | 75.5 |
| MsG0280006781.01.T01 | MTR_1g088925 | 38.202 | 89 | 55 | 0 | 1 | 89 | 7 | 95 | 3.58e-19 | 75.5 |
| MsG0280006781.01.T01 | MTR_7g108200 | 39.326 | 89 | 54 | 0 | 1 | 89 | 7 | 95 | 1.01e-18 | 74.3 |
| MsG0280006781.01.T01 | MTR_7g108220 | 36.634 | 101 | 55 | 2 | 1 | 96 | 7 | 103 | 1.07e-18 | 74.3 |
| MsG0280006781.01.T01 | MTR_7g108210 | 35.644 | 101 | 56 | 2 | 1 | 96 | 7 | 103 | 6.04e-18 | 72.4 |
| MsG0280006781.01.T01 | MTR_1g088910 | 33.663 | 101 | 57 | 3 | 1 | 96 | 7 | 102 | 9.54e-14 | 61.6 |
| MsG0280006781.01.T01 | MTR_3g077570 | 36.082 | 97 | 60 | 2 | 1 | 96 | 24 | 119 | 5.23e-13 | 60.5 |
| MsG0280006781.01.T01 | MTR_3g077560 | 33.684 | 95 | 61 | 2 | 1 | 94 | 27 | 120 | 4.91e-12 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006781.01.T01 | AT1G28480 | 64.000 | 100 | 32 | 1 | 1 | 96 | 38 | 137 | 1.76e-41 | 133 |
| MsG0280006781.01.T01 | AT5G14070 | 47.115 | 104 | 47 | 2 | 1 | 96 | 37 | 140 | 2.02e-29 | 102 |
| MsG0280006781.01.T01 | AT1G03850 | 48.958 | 96 | 45 | 1 | 1 | 96 | 59 | 150 | 6.54e-27 | 96.7 |
| MsG0280006781.01.T01 | AT3G02000 | 46.078 | 102 | 49 | 1 | 1 | 96 | 35 | 136 | 1.00e-25 | 93.2 |
| MsG0280006781.01.T01 | AT4G15700 | 45.545 | 101 | 45 | 3 | 1 | 96 | 7 | 102 | 1.13e-23 | 87.0 |
| MsG0280006781.01.T01 | AT4G15690 | 41.667 | 96 | 56 | 0 | 1 | 96 | 7 | 102 | 1.49e-23 | 86.7 |
| MsG0280006781.01.T01 | AT4G33040 | 47.525 | 101 | 47 | 2 | 1 | 96 | 45 | 144 | 1.51e-23 | 87.8 |
| MsG0280006781.01.T01 | AT4G15680 | 40.625 | 96 | 57 | 0 | 1 | 96 | 7 | 102 | 2.02e-23 | 86.3 |
| MsG0280006781.01.T01 | AT4G15670 | 39.583 | 96 | 58 | 0 | 1 | 96 | 7 | 102 | 5.12e-23 | 85.5 |
| MsG0280006781.01.T01 | AT4G15660 | 45.545 | 101 | 45 | 4 | 1 | 96 | 7 | 102 | 5.34e-23 | 85.5 |
| MsG0280006781.01.T01 | AT5G18600 | 41.667 | 96 | 56 | 0 | 1 | 96 | 7 | 102 | 2.14e-22 | 84.0 |
| MsG0280006781.01.T01 | AT3G21460 | 44.554 | 101 | 46 | 3 | 1 | 96 | 7 | 102 | 1.16e-20 | 79.3 |
| MsG0280006781.01.T01 | AT3G62950 | 37.113 | 97 | 60 | 1 | 1 | 96 | 7 | 103 | 1.94e-20 | 79.0 |
| MsG0280006781.01.T01 | AT2G30540 | 41.573 | 89 | 52 | 0 | 1 | 89 | 7 | 95 | 5.16e-20 | 77.8 |
| MsG0280006781.01.T01 | AT1G06830 | 41.573 | 89 | 52 | 0 | 1 | 89 | 7 | 95 | 8.41e-20 | 77.0 |
| MsG0280006781.01.T01 | AT1G03850 | 42.857 | 91 | 48 | 1 | 1 | 91 | 59 | 145 | 1.69e-19 | 78.2 |
| MsG0280006781.01.T01 | AT2G47880 | 40.449 | 89 | 53 | 0 | 1 | 89 | 7 | 95 | 4.82e-19 | 75.1 |
| MsG0280006781.01.T01 | AT5G11930 | 41.237 | 97 | 45 | 3 | 1 | 95 | 61 | 147 | 5.05e-19 | 76.6 |
| MsG0280006781.01.T01 | AT2G47870 | 35.644 | 101 | 56 | 2 | 1 | 96 | 7 | 103 | 1.62e-18 | 73.9 |
| MsG0280006781.01.T01 | AT3G62960 | 39.326 | 89 | 54 | 0 | 1 | 89 | 7 | 95 | 1.57e-17 | 71.2 |
| MsG0280006781.01.T01 | AT1G03020 | 30.208 | 96 | 67 | 0 | 1 | 96 | 7 | 102 | 3.49e-15 | 65.5 |
| MsG0280006781.01.T01 | AT3G62930 | 30.208 | 96 | 67 | 0 | 1 | 96 | 7 | 102 | 5.46e-15 | 65.1 |
| MsG0280006781.01.T01 | AT5G63030 | 37.895 | 95 | 57 | 2 | 1 | 94 | 25 | 118 | 9.11e-13 | 59.7 |
Find 33 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCCGAAACCGTTCAGTTTC+CGG | 0.216471 | 2:-6100010 | MsG0280006781.01.T01:CDS |
| GTCATGGCTACTCATATTTC+AGG | 0.236510 | 2:-6099946 | MsG0280006781.01.T01:CDS |
| TACTCATATTTCAGGTGAAT+TGG | 0.305467 | 2:-6099938 | MsG0280006781.01.T01:CDS |
| GAAAACGCAGTTATTATCAT+TGG | 0.318460 | 2:-6100159 | MsG0280006781.01.T01:CDS |
| CGGAGGTGTTGGTGGATAGT+TGG | 0.347866 | 2:+6100028 | None:intergenic |
| TGGGAAGTTGCTTGGTGGGT+TGG | 0.357470 | 2:-6099974 | MsG0280006781.01.T01:CDS |
| CGCCGGAAACTGAACGGTTT+CGG | 0.371997 | 2:+6100008 | None:intergenic |
| TTCGTTGGTGGGAAGTTGCT+TGG | 0.411854 | 2:-6099982 | MsG0280006781.01.T01:CDS |
| GGGCAGCAACGGCAGTTTCA+TGG | 0.413065 | 2:+6100049 | None:intergenic |
| CAGTTTCCGGCGGTGTTCGT+TGG | 0.424655 | 2:-6099997 | MsG0280006781.01.T01:CDS |
| TTTCCGGCGGTGTTCGTTGG+TGG | 0.452461 | 2:-6099994 | MsG0280006781.01.T01:CDS |
| TTGGTGGGAAGTTGCTTGGT+GGG | 0.461042 | 2:-6099978 | MsG0280006781.01.T01:CDS |
| GGAGGTGTTGGTGGATAGTT+GGG | 0.462970 | 2:+6100029 | None:intergenic |
| TTCCGGCGGTGTTCGTTGGT+GGG | 0.487798 | 2:-6099993 | MsG0280006781.01.T01:CDS |
| AACGGTTTCGGAGGTGTTGG+TGG | 0.499025 | 2:+6100020 | None:intergenic |
| GTTCCTATATTAAAAGATGC+TGG | 0.501499 | 2:-6099916 | MsG0280006781.01.T01:CDS |
| AAAAGATTGTTACAAGGTCT+AGG | 0.503022 | 2:-6100105 | MsG0280006781.01.T01:CDS |
| GTTGGTGGGAAGTTGCTTGG+TGG | 0.513182 | 2:-6099979 | MsG0280006781.01.T01:CDS |
| CTGAACGGTTTCGGAGGTGT+TGG | 0.524370 | 2:+6100017 | None:intergenic |
| TCTTGATCAACCTCGTAGAC+AGG | 0.528624 | 2:+6100072 | None:intergenic |
| GGTGGATAGTTGGGCAGCAA+CGG | 0.555191 | 2:+6100038 | None:intergenic |
| TTCCCACCAACGAACACCGC+CGG | 0.567247 | 2:+6099991 | None:intergenic |
| GTTGTCAAAAGATTGTTACA+AGG | 0.611660 | 2:-6100111 | MsG0280006781.01.T01:CDS |
| TGATCAACCTCGTAGACAGG+AGG | 0.639092 | 2:+6100075 | None:intergenic |
| GAACACCGCCGGAAACTGAA+CGG | 0.641793 | 2:+6100002 | None:intergenic |
| CGGAAACTGAACGGTTTCGG+AGG | 0.643135 | 2:+6100011 | None:intergenic |
| CGAAACCGTTCAGTTTCCGG+CGG | 0.644599 | 2:-6100007 | MsG0280006781.01.T01:CDS |
| TGGTGGGTTGGAAAGAGTCA+TGG | 0.645511 | 2:-6099962 | MsG0280006781.01.T01:CDS |
| GATCAACCTCGTAGACAGGA+GGG | 0.651727 | 2:+6100076 | None:intergenic |
| CATAACTAGTCAAAGCCACA+AGG | 0.660326 | 2:+6099891 | None:intergenic |
| TAAAAGATGCTGGTGCCTTG+TGG | 0.660431 | 2:-6099906 | MsG0280006781.01.T01:CDS |
| GTTATTATCATTGGAACACG+AGG | 0.684410 | 2:-6100150 | MsG0280006781.01.T01:CDS |
| AGTCAACCCTCCTGTCTACG+AGG | 0.688227 | 2:-6100082 | MsG0280006781.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAGATTGTTACAAGGTCT+AGG | - | Chr2:6099963-6099982 | MsG0280006781.01.T01:CDS | 30.0% | |
| GAAAACGCAGTTATTATCAT+TGG | - | Chr2:6099909-6099928 | MsG0280006781.01.T01:CDS | 30.0% | |
| GTTCCTATATTAAAAGATGC+TGG | - | Chr2:6100152-6100171 | MsG0280006781.01.T01:CDS | 30.0% | |
| GTTGTCAAAAGATTGTTACA+AGG | - | Chr2:6099957-6099976 | MsG0280006781.01.T01:CDS | 30.0% | |
| ! | GTAACAATCTTTTGACAACA+TGG | + | Chr2:6099956-6099975 | None:intergenic | 30.0% |
| ! | TACTCATATTTCAGGTGAAT+TGG | - | Chr2:6100130-6100149 | MsG0280006781.01.T01:CDS | 30.0% |
| GTTATTATCATTGGAACACG+AGG | - | Chr2:6099918-6099937 | MsG0280006781.01.T01:CDS | 35.0% | |
| ! | GCACCAGCATCTTTTAATAT+AGG | + | Chr2:6100158-6100177 | None:intergenic | 35.0% |
| GTCATGGCTACTCATATTTC+AGG | - | Chr2:6100122-6100141 | MsG0280006781.01.T01:CDS | 40.0% | |
| TAAAAGATGCTGGTGCCTTG+TGG | - | Chr2:6100162-6100181 | MsG0280006781.01.T01:CDS | 45.0% | |
| TCTTGATCAACCTCGTAGAC+AGG | + | Chr2:6099999-6100018 | None:intergenic | 45.0% | |
| CTCCGAAACCGTTCAGTTTC+CGG | - | Chr2:6100058-6100077 | MsG0280006781.01.T01:CDS | 50.0% | |
| GATCAACCTCGTAGACAGGA+GGG | + | Chr2:6099995-6100014 | None:intergenic | 50.0% | |
| TGATCAACCTCGTAGACAGG+AGG | + | Chr2:6099996-6100015 | None:intergenic | 50.0% | |
| TTGGTGGGAAGTTGCTTGGT+GGG | - | Chr2:6100090-6100109 | MsG0280006781.01.T01:CDS | 50.0% | |
| ! | TTCGTTGGTGGGAAGTTGCT+TGG | - | Chr2:6100086-6100105 | MsG0280006781.01.T01:CDS | 50.0% |
| !! | GGAGGTGTTGGTGGATAGTT+GGG | + | Chr2:6100042-6100061 | None:intergenic | 50.0% |
| !! | TGGTGGGTTGGAAAGAGTCA+TGG | - | Chr2:6100106-6100125 | MsG0280006781.01.T01:CDS | 50.0% |
| AGTCAACCCTCCTGTCTACG+AGG | - | Chr2:6099986-6100005 | MsG0280006781.01.T01:CDS | 55.0% | |
| CGAAACCGTTCAGTTTCCGG+CGG | - | Chr2:6100061-6100080 | MsG0280006781.01.T01:CDS | 55.0% | |
| CGCCGGAAACTGAACGGTTT+CGG | + | Chr2:6100063-6100082 | None:intergenic | 55.0% | |
| CGGAAACTGAACGGTTTCGG+AGG | + | Chr2:6100060-6100079 | None:intergenic | 55.0% | |
| GAACACCGCCGGAAACTGAA+CGG | + | Chr2:6100069-6100088 | None:intergenic | 55.0% | |
| GGTGGATAGTTGGGCAGCAA+CGG | + | Chr2:6100033-6100052 | None:intergenic | 55.0% | |
| ! | CTGAACGGTTTCGGAGGTGT+TGG | + | Chr2:6100054-6100073 | None:intergenic | 55.0% |
| ! | GTTGGTGGGAAGTTGCTTGG+TGG | - | Chr2:6100089-6100108 | MsG0280006781.01.T01:CDS | 55.0% |
| ! | TGGGAAGTTGCTTGGTGGGT+TGG | - | Chr2:6100094-6100113 | MsG0280006781.01.T01:CDS | 55.0% |
| !! | AACGGTTTCGGAGGTGTTGG+TGG | + | Chr2:6100051-6100070 | None:intergenic | 55.0% |
| !! | CGGAGGTGTTGGTGGATAGT+TGG | + | Chr2:6100043-6100062 | None:intergenic | 55.0% |
| CAGTTTCCGGCGGTGTTCGT+TGG | - | Chr2:6100071-6100090 | MsG0280006781.01.T01:CDS | 60.0% | |
| GGGCAGCAACGGCAGTTTCA+TGG | + | Chr2:6100022-6100041 | None:intergenic | 60.0% | |
| TTCCCACCAACGAACACCGC+CGG | + | Chr2:6100080-6100099 | None:intergenic | 60.0% | |
| TTCCGGCGGTGTTCGTTGGT+GGG | - | Chr2:6100075-6100094 | MsG0280006781.01.T01:CDS | 60.0% | |
| TTTCCGGCGGTGTTCGTTGG+TGG | - | Chr2:6100074-6100093 | MsG0280006781.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 6099900 | 6100190 | 6099900 | ID=MsG0280006781.01;Name=MsG0280006781.01 |
| Chr2 | mRNA | 6099900 | 6100190 | 6099900 | ID=MsG0280006781.01.T01;Parent=MsG0280006781.01;Name=MsG0280006781.01.T01;_AED=0.13;_eAED=0.13;_QI=0|-1|0|1|-1|1|1|0|96 |
| Chr2 | exon | 6099900 | 6100190 | 6099900 | ID=MsG0280006781.01.T01:exon:2512;Parent=MsG0280006781.01.T01 |
| Chr2 | CDS | 6099900 | 6100190 | 6099900 | ID=MsG0280006781.01.T01:cds;Parent=MsG0280006781.01.T01 |
| Gene Sequence |
| Protein sequence |
