AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380015986.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 34 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCTCTAAATGATTCAATT+TGG 0.226496 3:+77558468 MsG0380015986.01.T01:five_prime_UTR
AAGGCATCAAACATCAAATT+TGG 0.263688 3:+77558439 MsG0380015986.01.T01:five_prime_UTR
TCTTCTTTGATCAGGTCTTT+TGG 0.289740 3:-77558504 None:intergenic
TCAATCTTGGTTACAAGTTC+AGG 0.314165 3:+77558746 MsG0380015986.01.T01:CDS
TTTCTTATCAGCAGAAGTTT+TGG 0.316882 3:-77558872 None:intergenic
TCAAAGTTTCTTCTTTGATC+AGG 0.368380 3:-77558512 None:intergenic
CCACCAATGGTTTCATTCCT+AGG 0.370433 3:-77558925 None:intergenic
AAGATTTAACGAAATAAATA+AGG 0.374217 3:-77558306 None:intergenic
ATTCTCTTGACACCTTTGTT+AGG 0.385636 3:-77558797 None:intergenic
CGTTGTTGATGTTATTGTTA+AGG 0.397102 3:-77558714 None:intergenic
TTGTTAAGGTTGTTAACATA+AGG 0.399185 3:-77558700 None:intergenic
AACGTTAGCAACATCAATCT+TGG 0.407418 3:+77558733 MsG0380015986.01.T01:CDS
AATCTCAAAAGACAGATCTT+TGG 0.407703 3:+77558985 MsG0380015986.01.T01:CDS
CTTGTTGAATCCAGCAAAAG+AGG 0.416893 3:-77558662 None:intergenic
CTAACTGAATCTCTGTATCT+TGG 0.423011 3:-77559776 None:intergenic
GGCAATTTAGTGATCAACTA+AGG 0.427205 3:+77558420 MsG0380015986.01.T01:five_prime_UTR
CCATCATTGAGATCACTACT+TGG 0.466102 3:-77558583 None:intergenic
ATTGTTGCTATTGATGTCCA+TGG 0.490666 3:-77558384 None:intergenic
CCAAGTAGTGATCTCAATGA+TGG 0.491309 3:+77558583 MsG0380015986.01.T01:five_prime_UTR
AATGGTTTCATTCCTAGGTA+AGG 0.519328 3:-77558920 None:intergenic
ACTGAATCTCTGTATCTTGG+AGG 0.533877 3:-77559773 None:intergenic
AACAACATCAATAAGAAGCA+TGG 0.540510 3:+77558832 MsG0380015986.01.T01:CDS
GAAGATGATGAAAATTCCAA+TGG 0.549665 3:+77558609 MsG0380015986.01.T01:five_prime_UTR
CCTAGGAATGAAACCATTGG+TGG 0.571294 3:+77558925 MsG0380015986.01.T01:CDS
GTAGTGATCTCAATGATGGA+TGG 0.592204 3:+77558587 MsG0380015986.01.T01:five_prime_UTR
CAAAAGAGGAGTTGTTATTG+TGG 0.592639 3:-77558648 None:intergenic
TTACCTAGGAATGAAACCAT+TGG 0.599552 3:+77558922 MsG0380015986.01.T01:CDS
AACAAAGATATAGCCACCAA+TGG 0.615860 3:-77558938 None:intergenic
CTAGGTAAGGACTCAGATGG+TGG 0.634849 3:-77558907 None:intergenic
TTTGAAAGAAGAATCAACCA+TGG 0.636659 3:+77558367 MsG0380015986.01.T01:five_prime_UTR
TTCCTAGGTAAGGACTCAGA+TGG 0.649844 3:-77558910 None:intergenic
TGTTTGCAACAATGATACCA+TGG 0.671879 3:+77558957 MsG0380015986.01.T01:CDS
GAGTTTCAACTACCTAACAA+AGG 0.701587 3:+77558785 MsG0380015986.01.T01:CDS
CACCATCTGAGTCCTTACCT+AGG 0.710365 3:+77558908 MsG0380015986.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTACATATGTTTTTT+AGG + Chr3:77559056-77559075 MsG0380015986.01.T01:intron 10.0%
!!! CTTAAAATGTTAATTTTTAT+TGG - Chr3:77559236-77559255 None:intergenic 10.0%
!! AAGATTTAACGAAATAAATA+AGG - Chr3:77558309-77558328 None:intergenic 15.0%
!! TGAAATTTAATTGTGTAAAT+TGG + Chr3:77559709-77559728 MsG0380015986.01.T01:intron 15.0%
!! TTCAATGAAAAAAAAATGAT+TGG + Chr3:77559368-77559387 MsG0380015986.01.T01:intron 15.0%
!! TTTACACAATTAAATTTCAT+AGG - Chr3:77559708-77559727 None:intergenic 15.0%
!!! TGTTTTTATTTTCATATTTG+TGG + Chr3:77559114-77559133 MsG0380015986.01.T01:intron 15.0%
!! ACAATTAAATTTCATAGGAA+GGG - Chr3:77559703-77559722 None:intergenic 20.0%
!! TATCTCTAAATGATTCAATT+TGG + Chr3:77558468-77558487 MsG0380015986.01.T01:five_prime_UTR 20.0%
!!! ACATATGTTTTTTAGGTATA+GGG + Chr3:77559063-77559082 MsG0380015986.01.T01:intron 20.0%
!!! GATTGTGAATTTTATACAAT+AGG + Chr3:77559085-77559104 MsG0380015986.01.T01:intron 20.0%
!!! TAAATTGGTCTCTAAATTAT+TGG + Chr3:77559724-77559743 MsG0380015986.01.T01:intron 20.0%
!!! TACATATGTTTTTTAGGTAT+AGG + Chr3:77559062-77559081 MsG0380015986.01.T01:intron 20.0%
! AGTAATTCACTATGATGAAT+CGG + Chr3:77559408-77559427 MsG0380015986.01.T01:intron 25.0%
! CACAATTAAATTTCATAGGA+AGG - Chr3:77559704-77559723 None:intergenic 25.0%
! GGTTCAAAAAACAAATCTTT+CGG - Chr3:77559179-77559198 None:intergenic 25.0%
! TCATAATAAGATTCGATTCA+CGG - Chr3:77559200-77559219 None:intergenic 25.0%
! TTGTTAAGGTTGTTAACATA+AGG - Chr3:77558703-77558722 None:intergenic 25.0%
!! GTTTTCTTGGTAAATTTTGT+AGG + Chr3:77559746-77559765 MsG0380015986.01.T01:intron 25.0%
!!! CTCTAAATTATTGGTTTTCT+TGG + Chr3:77559733-77559752 MsG0380015986.01.T01:intron 25.0%
!!! GCAACAATGAAATTCTTTTT+TGG + Chr3:77558399-77558418 MsG0380015986.01.T01:five_prime_UTR 25.0%
!!! TTTTGAGTTGAAATTAGTGA+AGG - Chr3:77558563-77558582 None:intergenic 25.0%
AACAACATCAATAAGAAGCA+TGG + Chr3:77558832-77558851 MsG0380015986.01.T01:CDS 30.0%
AATCTCAAAAGACAGATCTT+TGG + Chr3:77558985-77559004 MsG0380015986.01.T01:CDS 30.0%
ACTGAATCATCAATAGTCTT+TGG + Chr3:77559621-77559640 MsG0380015986.01.T01:intron 30.0%
CGTTGTTGATGTTATTGTTA+AGG - Chr3:77558717-77558736 None:intergenic 30.0%
GAAGATGATGAAAATTCCAA+TGG + Chr3:77558609-77558628 MsG0380015986.01.T01:five_prime_UTR 30.0%
GTAATTCACTATGATGAATC+GGG + Chr3:77559409-77559428 MsG0380015986.01.T01:intron 30.0%
TATTGTGGCATGAAAAAAAG+TGG - Chr3:77558636-77558655 None:intergenic 30.0%
TCAAAGTTTCTTCTTTGATC+AGG - Chr3:77558515-77558534 None:intergenic 30.0%
TCTTCCATGTTGAAAATTAG+AGG + Chr3:77559648-77559667 MsG0380015986.01.T01:intron 30.0%
TTTGAAAGAAGAATCAACCA+TGG + Chr3:77558367-77558386 MsG0380015986.01.T01:five_prime_UTR 30.0%
! AAGGCATCAAACATCAAATT+TGG + Chr3:77558439-77558458 MsG0380015986.01.T01:five_prime_UTR 30.0%
! AGTTGCAAGATTCTGAAAAA+TGG - Chr3:77558335-77558354 None:intergenic 30.0%
! TCTTCCTCTAATTTTCAACA+TGG - Chr3:77559655-77559674 None:intergenic 30.0%
! TGATTGGCTATACTAAGTTT+AGG + Chr3:77559384-77559403 MsG0380015986.01.T01:intron 30.0%
! TTTCTTATCAGCAGAAGTTT+TGG - Chr3:77558875-77558894 None:intergenic 30.0%
AACAAAGATATAGCCACCAA+TGG - Chr3:77558941-77558960 None:intergenic 35.0%
AACGTTAGCAACATCAATCT+TGG + Chr3:77558733-77558752 MsG0380015986.01.T01:CDS 35.0%
AATGGTTTCATTCCTAGGTA+AGG - Chr3:77558923-77558942 None:intergenic 35.0%
ATTCTCTTGACACCTTTGTT+AGG - Chr3:77558800-77558819 None:intergenic 35.0%
CATGAAAAAAAGTGGTCCAT+TGG - Chr3:77558628-77558647 None:intergenic 35.0%
CTAACTGAATCTCTGTATCT+TGG - Chr3:77559779-77559798 None:intergenic 35.0%
GAGTTTCAACTACCTAACAA+AGG + Chr3:77558785-77558804 MsG0380015986.01.T01:CDS 35.0%
GGCAATTTAGTGATCAACTA+AGG + Chr3:77558420-77558439 MsG0380015986.01.T01:five_prime_UTR 35.0%
TCAATCTTGGTTACAAGTTC+AGG + Chr3:77558746-77558765 MsG0380015986.01.T01:CDS 35.0%
TGAATCTCGATTCACTATGA+TGG - Chr3:77559437-77559456 None:intergenic 35.0%
TGTTTGCAACAATGATACCA+TGG + Chr3:77558957-77558976 MsG0380015986.01.T01:CDS 35.0%
TTACCTAGGAATGAAACCAT+TGG + Chr3:77558922-77558941 MsG0380015986.01.T01:CDS 35.0%
TTCATTGAACTTTGCAAAGC+TGG - Chr3:77559357-77559376 None:intergenic 35.0%
TTTGTAAGATCTCAGTTCCA+TGG - Chr3:77559593-77559612 None:intergenic 35.0%
! AATAACAACTCCTCTTTTGC+TGG + Chr3:77558652-77558671 MsG0380015986.01.T01:five_prime_UTR 35.0%
! ACACTAAAGATCGTATCGAT+TGG + Chr3:77559505-77559524 MsG0380015986.01.T01:intron 35.0%
! ATTGTTGCTATTGATGTCCA+TGG - Chr3:77558387-77558406 None:intergenic 35.0%
! CAAAAGAGGAGTTGTTATTG+TGG - Chr3:77558651-77558670 None:intergenic 35.0%
! TCTTCTTTGATCAGGTCTTT+TGG - Chr3:77558507-77558526 None:intergenic 35.0%
! TCTTTTGCTGGATTCAACAA+GGG + Chr3:77558664-77558683 MsG0380015986.01.T01:five_prime_UTR 35.0%
!! CACTAAAGATCGTATCGATT+GGG + Chr3:77559506-77559525 MsG0380015986.01.T01:intron 35.0%
!! GGTTACAAGTTCAGGTTTTA+AGG + Chr3:77558754-77558773 MsG0380015986.01.T01:CDS 35.0%
!!! ACAAGTTCAGGTTTTAAGGT+TGG + Chr3:77558758-77558777 MsG0380015986.01.T01:CDS 35.0%
ACTGAATCTCTGTATCTTGG+AGG - Chr3:77559776-77559795 None:intergenic 40.0%
CCAAGTAGTGATCTCAATGA+TGG + Chr3:77558583-77558602 MsG0380015986.01.T01:five_prime_UTR 40.0%
CCATCATTGAGATCACTACT+TGG - Chr3:77558586-77558605 None:intergenic 40.0%
CTTGTTGAATCCAGCAAAAG+AGG - Chr3:77558665-77558684 None:intergenic 40.0%
! CTCTTTTGCTGGATTCAACA+AGG + Chr3:77558663-77558682 MsG0380015986.01.T01:five_prime_UTR 40.0%
! GTAGTGATCTCAATGATGGA+TGG + Chr3:77558587-77558606 MsG0380015986.01.T01:five_prime_UTR 40.0%
!!! TCTTTTGAGATTCTCAGCCA+TGG - Chr3:77558977-77558996 None:intergenic 40.0%
CCACCAATGGTTTCATTCCT+AGG - Chr3:77558928-77558947 None:intergenic 45.0%
CCTAGGAATGAAACCATTGG+TGG + Chr3:77558925-77558944 MsG0380015986.01.T01:CDS 45.0%
GATACATGTGAGTGTGACCA+TGG + Chr3:77559573-77559592 MsG0380015986.01.T01:intron 45.0%
TGAACTTTGCAAAGCTGGTC+AGG - Chr3:77559352-77559371 None:intergenic 45.0%
TTCCTAGGTAAGGACTCAGA+TGG - Chr3:77558913-77558932 None:intergenic 45.0%
! ATCAGGTCTTTTGGTCACGT+TGG - Chr3:77558498-77558517 None:intergenic 45.0%
CACCATCTGAGTCCTTACCT+AGG + Chr3:77558908-77558927 MsG0380015986.01.T01:CDS 50.0%
CTAGGTAAGGACTCAGATGG+TGG - Chr3:77558910-77558929 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr3 gene 77558299 77559806 77558299 ID=MsG0380015986.01;Name=MsG0380015986.01
Chr3 mRNA 77558299 77559806 77558299 ID=MsG0380015986.01.T01;Parent=MsG0380015986.01;Name=MsG0380015986.01.T01;_AED=0.25;_eAED=0.25;_QI=382|0.8|0.83|1|0.2|0.33|6|0|113
Chr3 exon 77558299 77558335 77558299 ID=MsG0380015986.01.T01:exon:1430;Parent=MsG0380015986.01.T01
Chr3 exon 77558359 77559006 77558359 ID=MsG0380015986.01.T01:exon:1431;Parent=MsG0380015986.01.T01
Chr3 exon 77559768 77559806 77559768 ID=MsG0380015986.01.T01:exon:1432;Parent=MsG0380015986.01.T01
Chr3 five_prime_UTR 77558299 77558335 77558299 ID=MsG0380015986.01.T01:five_prime_utr;Parent=MsG0380015986.01.T01
Chr3 five_prime_UTR 77558359 77558703 77558359 ID=MsG0380015986.01.T01:five_prime_utr;Parent=MsG0380015986.01.T01
Chr3 CDS 77558704 77559006 77558704 ID=MsG0380015986.01.T01:cds;Parent=MsG0380015986.01.T01
Chr3 CDS 77559768 77559806 77559768 ID=MsG0380015986.01.T01:cds;Parent=MsG0380015986.01.T01
Gene Sequence

>MsG0380015986.01.T01

ATGTTAACAACCTTAACAATAACATCAACAACGTTAGCAACATCAATCTTGGTTACAAGTTCAGGTTTTAAGGTTGGTGATGAGTTTCAACTACCTAACAAAGGTGTCAAGAGAATATCAACAGCAATAACAACATCAATAAGAAGCATGGAGATAACACTGATGTTACCAAAACTTCTGCTGATAAGAAATTCAAAACTTTGCCACCATCTGAGTCCTTACCTAGGAATGAAACCATTGGTGGCTATATCTTTGTTTGCAACAATGATACCATGGCTGAGAATCTCAAAAGACAGATCTTTGGTCTGCCTCCAAGATACAGAGATTCAGTTAGAACAATAA

Protein sequence

>MsG0380015986.01.T01

MLTTLTITSTTLATSILVTSSGFKVGDEFQLPNKGVKRISTAITTSIRSMEITLMLPKLLLIRNSKLCHHLSPYLGMKPLVAISLFATMIPWLRISKDRSLVCLQDTEIQLEQ*