Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007252.01.T01 | XP_013462831.1 | 94.667 | 150 | 8 | 0 | 1 | 150 | 7 | 156 | 3.77E-77 | 236 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007252.01.T01 | A0A072V5G6 | 94.667 | 150 | 8 | 0 | 1 | 150 | 7 | 156 | 1.80e-77 | 236 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048357.01 | MsG0280007252.01 | 0.805882 | 1.077167e-49 | 5.764258e-47 |
| MsG0180001771.01 | MsG0280007252.01 | 0.816385 | 5.682909e-52 | 4.020992e-49 |
| MsG0280007252.01 | MsG0280007305.01 | 0.813740 | 2.195948e-51 | 1.445861e-48 |
| MsG0280007252.01 | MsG0280007414.01 | 0.810695 | 1.014454e-50 | 6.157173e-48 |
| MsG0280007252.01 | MsG0580024443.01 | 0.810714 | 1.005001e-50 | 6.102867e-48 |
| MsG0280007252.01 | MsG0580029417.01 | 0.815469 | 9.097978e-52 | 6.278435e-49 |
| MsG0280007252.01 | MsG0580029418.01 | 0.845210 | 4.680026e-59 | 7.742558e-56 |
| MsG0280007252.01 | MsG0680031707.01 | 0.840428 | 8.747034e-58 | 1.245546e-54 |
| MsG0280007252.01 | MsG0880041858.01 | 0.836049 | 1.174918e-56 | 1.463438e-53 |
| MsG0280007252.01 | MsG0880042958.01 | 0.802919 | 4.464173e-49 | 2.213750e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007252.01.T01 | MTR_2g023390 | 94.667 | 150 | 8 | 0 | 1 | 150 | 7 | 156 | 4.57e-81 | 236 |
| MsG0280007252.01.T01 | MTR_3g052330 | 39.106 | 179 | 69 | 4 | 12 | 150 | 20 | 198 | 8.56e-31 | 110 |
| MsG0280007252.01.T01 | MTR_2g094410 | 53.488 | 86 | 32 | 2 | 67 | 148 | 73 | 154 | 1.27e-21 | 85.5 |
| MsG0280007252.01.T01 | MTR_4g132020 | 37.654 | 162 | 75 | 7 | 4 | 148 | 22 | 174 | 3.71e-21 | 84.7 |
| MsG0280007252.01.T01 | MTR_4g059320 | 50.549 | 91 | 38 | 2 | 61 | 148 | 71 | 157 | 1.93e-20 | 82.4 |
| MsG0280007252.01.T01 | MTR_2g023070 | 44.211 | 95 | 46 | 2 | 58 | 148 | 125 | 216 | 3.14e-16 | 72.8 |
| MsG0280007252.01.T01 | MTR_7g101250 | 38.514 | 148 | 71 | 4 | 7 | 148 | 25 | 158 | 3.73e-16 | 71.2 |
| MsG0280007252.01.T01 | MTR_2g018175 | 45.977 | 87 | 31 | 3 | 68 | 150 | 52 | 126 | 6.94e-16 | 69.7 |
| MsG0280007252.01.T01 | MTR_2g086660 | 38.202 | 89 | 46 | 2 | 68 | 150 | 170 | 255 | 1.04e-15 | 72.0 |
| MsG0280007252.01.T01 | MTR_2g086660 | 38.202 | 89 | 46 | 2 | 68 | 150 | 170 | 255 | 1.34e-14 | 69.3 |
| MsG0280007252.01.T01 | MTR_7g105130 | 58.333 | 48 | 20 | 0 | 103 | 150 | 170 | 217 | 2.90e-13 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007252.01.T01 | AT4G04630 | 53.205 | 156 | 49 | 5 | 10 | 150 | 22 | 168 | 6.75e-39 | 130 |
| MsG0280007252.01.T01 | AT4G21970 | 68.966 | 87 | 24 | 1 | 67 | 150 | 59 | 145 | 3.33e-38 | 127 |
| MsG0280007252.01.T01 | AT3G45210 | 51.852 | 81 | 32 | 3 | 70 | 150 | 75 | 148 | 1.24e-22 | 87.8 |
| MsG0280007252.01.T01 | AT3G15040 | 45.455 | 110 | 49 | 3 | 49 | 150 | 137 | 243 | 8.44e-19 | 80.1 |
| MsG0280007252.01.T01 | AT1G11700 | 50.575 | 87 | 37 | 3 | 67 | 148 | 114 | 199 | 2.29e-18 | 78.2 |
| MsG0280007252.01.T01 | AT1G29640 | 38.983 | 118 | 57 | 5 | 35 | 150 | 35 | 139 | 1.32e-17 | 74.7 |
| MsG0280007252.01.T01 | AT2G28400 | 47.253 | 91 | 36 | 3 | 66 | 148 | 74 | 160 | 3.57e-17 | 73.9 |
| MsG0280007252.01.T01 | AT4G26950 | 39.041 | 146 | 68 | 6 | 12 | 150 | 13 | 144 | 6.03e-17 | 73.2 |
| MsG0280007252.01.T01 | AT4G26950 | 39.041 | 146 | 68 | 6 | 12 | 150 | 13 | 144 | 6.03e-17 | 73.2 |
| MsG0280007252.01.T01 | AT5G60680 | 42.254 | 142 | 63 | 6 | 14 | 148 | 32 | 161 | 2.96e-16 | 71.6 |
| MsG0280007252.01.T01 | AT1G61930 | 47.191 | 89 | 39 | 3 | 67 | 148 | 114 | 201 | 8.55e-16 | 71.2 |
| MsG0280007252.01.T01 | AT2G34340 | 44.186 | 86 | 43 | 3 | 67 | 150 | 53 | 135 | 1.68e-15 | 68.9 |
| MsG0280007252.01.T01 | AT5G03230 | 46.154 | 91 | 35 | 2 | 68 | 148 | 78 | 164 | 6.79e-14 | 65.5 |
| MsG0280007252.01.T01 | AT4G21930 | 39.560 | 91 | 44 | 3 | 67 | 148 | 93 | 181 | 2.93e-13 | 64.3 |
Find 30 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTGATTGGTCAAAAGTTTA+TGG | 0.133639 | 2:+12577274 | MsG0280007252.01.T01:CDS |
| GAAGATGTATGGGGTGATTT+TGG | 0.274037 | 2:+12577106 | MsG0280007252.01.T01:CDS |
| CTACTCAATGAAGCCAGTTT+TGG | 0.325619 | 2:-12577485 | None:intergenic |
| CATCTTCTTCATCAAAATCT+TGG | 0.359385 | 2:-12577090 | None:intergenic |
| AAAGGTAGTGTATTAGCCTT+TGG | 0.400912 | 2:-12577199 | None:intergenic |
| GACCAATCAGGAATGTTGAT+AGG | 0.429178 | 2:-12577262 | None:intergenic |
| TTGGCTCAAGAAAGATCAAA+AGG | 0.430628 | 2:+12577125 | MsG0280007252.01.T01:CDS |
| GAAGAGATCTTAGCAAAGTA+AGG | 0.446787 | 2:+12577449 | MsG0280007252.01.T01:CDS |
| GCTGAAGAACCTTCAACAAA+AGG | 0.453707 | 2:-12577238 | None:intergenic |
| CATCTTTCTCTGTGTGTGAA+GGG | 0.455421 | 2:+12577407 | MsG0280007252.01.T01:CDS |
| TCATCTTTCTCTGTGTGTGA+AGG | 0.455817 | 2:+12577406 | MsG0280007252.01.T01:CDS |
| AGAAGAGCTAGAGAAATCCT+TGG | 0.484917 | 2:-12577152 | None:intergenic |
| CTCCTATCAACATTCCTGAT+TGG | 0.492143 | 2:+12577260 | MsG0280007252.01.T01:CDS |
| AAAGATCAAAAGGGCTTCCA+AGG | 0.493591 | 2:+12577135 | MsG0280007252.01.T01:CDS |
| TTTCTCTGTGTGTGAAGGGA+TGG | 0.495055 | 2:+12577411 | MsG0280007252.01.T01:CDS |
| ACATGGTTCCTCCACATGAA+TGG | 0.496619 | 2:+12577353 | MsG0280007252.01.T01:CDS |
| TTCTCTGTGTGTGAAGGGAT+GGG | 0.526082 | 2:+12577412 | MsG0280007252.01.T01:CDS |
| CTGAAGAACCTTCAACAAAA+GGG | 0.533366 | 2:-12577237 | None:intergenic |
| GGGTCATCAGAAACAACAAA+AGG | 0.552733 | 2:-12577217 | None:intergenic |
| TGGCTCAAGAAAGATCAAAA+GGG | 0.553109 | 2:+12577126 | MsG0280007252.01.T01:CDS |
| TTAGCAATCCATTCATGTGG+AGG | 0.597425 | 2:-12577361 | None:intergenic |
| GGGATGGGAAGGACACTCAA+AGG | 0.610671 | 2:+12577427 | MsG0280007252.01.T01:CDS |
| GATGAAGATGGTGATGAGGA+TGG | 0.611115 | 2:+12577328 | MsG0280007252.01.T01:CDS |
| TTCTTAGCAATCCATTCATG+TGG | 0.614036 | 2:-12577364 | None:intergenic |
| GGATTGCTAAGAAGCTTGCA+AGG | 0.624867 | 2:+12577374 | MsG0280007252.01.T01:CDS |
| TGATGATGAAGATGGTGATG+AGG | 0.647712 | 2:+12577324 | MsG0280007252.01.T01:CDS |
| CTGTGTGTGAAGGGATGGGA+AGG | 0.653389 | 2:+12577416 | MsG0280007252.01.T01:CDS |
| TGGTGATGAGGATGGTGACA+TGG | 0.661072 | 2:+12577336 | MsG0280007252.01.T01:CDS |
| GGTTTGCATGATGATGAAGA+TGG | 0.676486 | 2:+12577316 | MsG0280007252.01.T01:CDS |
| TTTGATGAAGAAGATGTATG+GGG | 0.717521 | 2:+12577097 | MsG0280007252.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGTAGAAAAAATATTCCAA+AGG | + | Chr2:12577183-12577202 | MsG0280007252.01.T01:CDS | 20.0% |
| ! | TATGGTAAGAATAGCAAAAA+TGG | + | Chr2:12577292-12577311 | MsG0280007252.01.T01:CDS | 25.0% |
| !! | TTTTGATGAAGAAGATGTAT+GGG | + | Chr2:12577096-12577115 | MsG0280007252.01.T01:CDS | 25.0% |
| !!! | AATATTTTTTCTACATGCAG+AGG | - | Chr2:12577179-12577198 | None:intergenic | 25.0% |
| !!! | ATTTTGATGAAGAAGATGTA+TGG | + | Chr2:12577095-12577114 | MsG0280007252.01.T01:CDS | 25.0% |
| CATCTTCTTCATCAAAATCT+TGG | - | Chr2:12577093-12577112 | None:intergenic | 30.0% | |
| GAACAAAAACACTTCATCAA+AGG | + | Chr2:12577060-12577079 | MsG0280007252.01.T01:CDS | 30.0% | |
| TTTGATGAAGAAGATGTATG+GGG | + | Chr2:12577097-12577116 | MsG0280007252.01.T01:CDS | 30.0% | |
| !!! | AATGCTATTTTGACCAAAAC+TGG | + | Chr2:12577472-12577491 | MsG0280007252.01.T01:CDS | 30.0% |
| CTGAAGAACCTTCAACAAAA+GGG | - | Chr2:12577240-12577259 | None:intergenic | 35.0% | |
| GAAGAGATCTTAGCAAAGTA+AGG | + | Chr2:12577449-12577468 | MsG0280007252.01.T01:CDS | 35.0% | |
| GGTAAGAATAGCAAAAATGG+TGG | + | Chr2:12577295-12577314 | MsG0280007252.01.T01:CDS | 35.0% | |
| TGGCTCAAGAAAGATCAAAA+GGG | + | Chr2:12577126-12577145 | MsG0280007252.01.T01:CDS | 35.0% | |
| TTCTTAGCAATCCATTCATG+TGG | - | Chr2:12577367-12577386 | None:intergenic | 35.0% | |
| TTGGCTCAAGAAAGATCAAA+AGG | + | Chr2:12577125-12577144 | MsG0280007252.01.T01:CDS | 35.0% | |
| ! | CCATAAACTTTTGACCAATC+AGG | - | Chr2:12577277-12577296 | None:intergenic | 35.0% |
| ! | CCTGATTGGTCAAAAGTTTA+TGG | + | Chr2:12577274-12577293 | MsG0280007252.01.T01:CDS | 35.0% |
| !! | AAAGGTAGTGTATTAGCCTT+TGG | - | Chr2:12577202-12577221 | None:intergenic | 35.0% |
| AAAGATCAAAAGGGCTTCCA+AGG | + | Chr2:12577135-12577154 | MsG0280007252.01.T01:CDS | 40.0% | |
| AGAAGAGCTAGAGAAATCCT+TGG | - | Chr2:12577155-12577174 | None:intergenic | 40.0% | |
| CATCTTTCTCTGTGTGTGAA+GGG | + | Chr2:12577407-12577426 | MsG0280007252.01.T01:CDS | 40.0% | |
| CTCCTATCAACATTCCTGAT+TGG | + | Chr2:12577260-12577279 | MsG0280007252.01.T01:CDS | 40.0% | |
| GACCAATCAGGAATGTTGAT+AGG | - | Chr2:12577265-12577284 | None:intergenic | 40.0% | |
| GCTGAAGAACCTTCAACAAA+AGG | - | Chr2:12577241-12577260 | None:intergenic | 40.0% | |
| GGGTCATCAGAAACAACAAA+AGG | - | Chr2:12577220-12577239 | None:intergenic | 40.0% | |
| TCATCTTTCTCTGTGTGTGA+AGG | + | Chr2:12577406-12577425 | MsG0280007252.01.T01:CDS | 40.0% | |
| TTAGCAATCCATTCATGTGG+AGG | - | Chr2:12577364-12577383 | None:intergenic | 40.0% | |
| ! | CTACTCAATGAAGCCAGTTT+TGG | - | Chr2:12577488-12577507 | None:intergenic | 40.0% |
| ! | GAAGATGTATGGGGTGATTT+TGG | + | Chr2:12577106-12577125 | MsG0280007252.01.T01:CDS | 40.0% |
| ! | GGTTTGCATGATGATGAAGA+TGG | + | Chr2:12577316-12577335 | MsG0280007252.01.T01:CDS | 40.0% |
| ! | TCTGATGACCCTTTTGTTGA+AGG | + | Chr2:12577229-12577248 | MsG0280007252.01.T01:CDS | 40.0% |
| ! | TGATGATGAAGATGGTGATG+AGG | + | Chr2:12577324-12577343 | MsG0280007252.01.T01:CDS | 40.0% |
| ACATGGTTCCTCCACATGAA+TGG | + | Chr2:12577353-12577372 | MsG0280007252.01.T01:CDS | 45.0% | |
| GGATTGCTAAGAAGCTTGCA+AGG | + | Chr2:12577374-12577393 | MsG0280007252.01.T01:CDS | 45.0% | |
| TTCTCTGTGTGTGAAGGGAT+GGG | + | Chr2:12577412-12577431 | MsG0280007252.01.T01:CDS | 45.0% | |
| TTTCTCTGTGTGTGAAGGGA+TGG | + | Chr2:12577411-12577430 | MsG0280007252.01.T01:CDS | 45.0% | |
| ! | GATGAAGATGGTGATGAGGA+TGG | + | Chr2:12577328-12577347 | MsG0280007252.01.T01:CDS | 45.0% |
| ! | TGGTGATGAGGATGGTGACA+TGG | + | Chr2:12577336-12577355 | MsG0280007252.01.T01:CDS | 50.0% |
| CTGTGTGTGAAGGGATGGGA+AGG | + | Chr2:12577416-12577435 | MsG0280007252.01.T01:CDS | 55.0% | |
| GGGATGGGAAGGACACTCAA+AGG | + | Chr2:12577427-12577446 | MsG0280007252.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 12577055 | 12577507 | 12577055 | ID=MsG0280007252.01;Name=MsG0280007252.01 |
| Chr2 | mRNA | 12577055 | 12577507 | 12577055 | ID=MsG0280007252.01.T01;Parent=MsG0280007252.01;Name=MsG0280007252.01.T01;_AED=0.21;_eAED=0.21;_QI=0|-1|0|1|-1|1|1|0|150 |
| Chr2 | exon | 12577055 | 12577507 | 12577055 | ID=MsG0280007252.01.T01:exon:7605;Parent=MsG0280007252.01.T01 |
| Chr2 | CDS | 12577055 | 12577507 | 12577055 | ID=MsG0280007252.01.T01:cds;Parent=MsG0280007252.01.T01 |
| Gene Sequence |
| Protein sequence |
