AlfalfaGEDB | MsG0280007305.01

M. sativa cultivar ZhongmuNo.1 / MsG0280007305.01

KEGG: -
GO: -
PFAM: PF04852

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007305.01.T01	XP_013450572.1	97.647	170	3	1	1	169	1	170	3.41E-120	346

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007305.01.T01	Q9FGH2	70.423	142	36	4	26	163	18	157	1.67E-66	203

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007305.01.T01	I3T2Z0	97.647	170	3	1	1	169	1	170	1.63e-120	346

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048357.01	MsG0280007305.01	0.909706	4.232623e-82	8.339962e-78
MsG0180000632.01	MsG0280007305.01	0.863551	2.296656e-64	6.989445e-61
MsG0180001200.01	MsG0280007305.01	0.889789	1.767863e-73	1.446922e-69
MsG0180001771.01	MsG0280007305.01	0.886793	2.509487e-72	1.818143e-68
MsG0180003431.01	MsG0280007305.01	0.828198	1.027177e-54	1.013897e-51
MsG0180003741.01	MsG0280007305.01	0.840203	1.001854e-57	1.416749e-54
MsG0280007252.01	MsG0280007305.01	0.813740	2.195948e-51	1.445861e-48
MsG0280007305.01	MsG0280007414.01	0.891518	3.692178e-74	3.248029e-70
MsG0280007305.01	MsG0280008390.01	0.809260	2.066015e-50	1.207280e-47
MsG0280007305.01	MsG0280008391.01	0.809039	2.303799e-50	1.338279e-47
MsG0280007305.01	MsG0280008392.01	0.818300	2.106295e-52	1.570631e-49
MsG0280007305.01	MsG0280008395.01	0.825315	5.013525e-54	4.553884e-51
MsG0280007305.01	MsG0280010163.01	0.822135	2.784515e-53	2.310627e-50
MsG0280007305.01	MsG0280010320.01	0.827135	1.849364e-54	1.769601e-51
MsG0280007305.01	MsG0380011496.01	0.832340	9.996635e-56	1.113983e-52
MsG0280007305.01	MsG0380011501.01	0.813280	2.772587e-51	1.802818e-48
MsG0280007305.01	MsG0380011675.01	0.809620	1.729401e-50	1.020387e-47
MsG0280007305.01	MsG0380016465.01	0.874081	8.921297e-68	3.961970e-64
MsG0280007305.01	MsG0380016466.01	0.832160	1.107656e-55	1.227822e-52
MsG0280007305.01	MsG0380017823.01	0.844442	7.539260e-59	1.217275e-55
MsG0280007305.01	MsG0380017869.01	0.813490	2.493551e-51	1.630763e-48
MsG0280007305.01	MsG0480018909.01	0.852329	4.958924e-61	1.032635e-57
MsG0280007305.01	MsG0480019279.01	0.830342	3.099132e-55	3.256255e-52
MsG0280007305.01	MsG0480020096.01	0.828048	1.116183e-54	1.096942e-51
MsG0280007305.01	MsG0480024022.01	0.826831	2.186187e-54	2.073386e-51
MsG0280007305.01	MsG0580024094.01	0.810078	1.378344e-50	8.230280e-48
MsG0280007305.01	MsG0580025974.01	0.800284	1.549412e-48	7.186486e-46
MsG0280007305.01	MsG0580027925.01	0.819499	1.124943e-52	8.671723e-50
MsG0280007305.01	MsG0580029310.01	0.857100	2.054182e-62	5.012508e-59
MsG0280007305.01	MsG0580029405.01	0.859975	2.851275e-63	7.670932e-60
MsG0280007305.01	MsG0580029406.01	0.837880	4.002432e-57	5.270601e-54
MsG0280007305.01	MsG0580029417.01	0.927965	5.619160e-92	2.886850e-87
MsG0280007305.01	MsG0580029418.01	0.953309	3.639426e-111	9.475992e-106
MsG0280007305.01	MsG0680031342.01	0.835079	2.066976e-56	2.500337e-53
MsG0280007305.01	MsG0680031707.01	0.933680	1.297137e-95	9.352309e-91
MsG0280007305.01	MsG0680033143.01	0.837161	6.119386e-57	7.881476e-54
MsG0280007305.01	MsG0680035658.01	0.811211	7.840856e-51	4.824717e-48
MsG0280007305.01	MsG0680035683.01	0.802115	6.538578e-49	3.176588e-46
MsG0280007305.01	MsG0780036148.01	0.825502	4.526524e-54	4.133263e-51
MsG0280007305.01	MsG0780038048.01	0.806502	7.973808e-50	4.335002e-47
MsG0280007305.01	MsG0780040846.01	0.860461	2.033350e-63	5.562797e-60
MsG0280007305.01	MsG0780040966.01	0.860891	1.505783e-63	4.180337e-60
MsG0280007305.01	MsG0780041259.01	0.838464	2.831963e-57	3.796207e-54
MsG0280007305.01	MsG0780041676.01	0.826255	2.999203e-54	2.798071e-51
MsG0280007305.01	MsG0780041698.01	0.829675	4.508507e-55	4.646639e-52
MsG0280007305.01	MsG0880041858.01	0.873614	1.282560e-67	5.600718e-64
MsG0280007305.01	MsG0880042442.01	0.849301	3.530593e-60	6.664003e-57
MsG0280007305.01	MsG0880042958.01	0.834196	3.447470e-56	4.061142e-53
MsG0280007305.01	MsG0880042959.01	0.832347	9.955199e-56	1.109633e-52
MsG0280007305.01	MsG0880045585.01	0.843479	1.366510e-58	2.140735e-55
MsG0280007305.01	MsG0880045586.01	0.857915	1.178988e-62	2.956941e-59
MsG0280007305.01	MsG0880046942.01	0.800660	1.298899e-48	6.082256e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007305.01.T01	MTR_7g115700	97.647	170	3	1	1	169	1	170	4.13e-124	346
MsG0280007305.01.T01	MTR_5g072510	72.263	137	31	4	26	158	36	169	1.11e-67	205
MsG0280007305.01.T01	MTR_4g094428	68.571	140	37	4	25	160	44	180	2.99e-64	197
MsG0280007305.01.T01	MTR_1g442860	73.438	128	28	3	27	151	65	189	4.23e-64	196
MsG0280007305.01.T01	MTR_2g016110	71.318	129	31	3	25	150	33	158	9.40e-63	191
MsG0280007305.01.T01	MTR_1g080210	73.288	146	33	3	27	169	40	182	2.18e-62	191
MsG0280007305.01.T01	MTR_2g092950	65.306	147	45	3	16	159	12	155	4.55e-62	189
MsG0280007305.01.T01	MTR_2g076400	68.217	129	35	3	25	150	31	156	5.34e-60	184
MsG0280007305.01.T01	MTR_1g075990	70.213	141	36	3	19	156	45	182	9.52e-60	184
MsG0280007305.01.T01	MTR_4g079930	66.667	129	37	3	25	150	27	152	1.86e-59	183
MsG0280007305.01.T01	MTR_7g097030	72.263	137	32	3	23	156	60	193	4.76e-59	183
MsG0280007305.01.T01	MTR_1g069825	73.684	133	29	3	27	156	52	181	1.55e-58	182
MsG0280007305.01.T01	MTR_3g031830	68.276	145	39	4	27	167	42	183	1.52e-56	177
MsG0280007305.01.T01	MTR_8g086350	64.865	148	43	5	25	167	29	172	3.58e-48	154
MsG0280007305.01.T01	MTR_1g114020	65.385	52	17	1	20	71	64	114	6.19e-17	74.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007305.01.T01	AT5G58500	70.423	142	36	4	26	163	18	157	1.70e-67	203
MsG0280007305.01.T01	AT1G07090	65.493	142	43	3	26	164	30	168	1.55e-64	196
MsG0280007305.01.T01	AT5G28490	76.471	136	26	3	27	159	25	157	1.67e-62	191
MsG0280007305.01.T01	AT3G04510	70.714	140	35	3	27	163	33	169	8.89e-60	184
MsG0280007305.01.T01	AT2G42610	67.969	128	35	3	26	150	24	148	2.64e-59	182
MsG0280007305.01.T01	AT2G42610	67.969	128	35	3	26	150	24	148	2.64e-59	182
MsG0280007305.01.T01	AT2G31160	75.556	135	26	4	27	157	54	185	4.71e-58	181
MsG0280007305.01.T01	AT4G18610	65.116	129	38	3	26	150	36	161	2.26e-57	178
MsG0280007305.01.T01	AT3G23290	75.573	131	26	3	23	150	44	171	2.79e-57	178
MsG0280007305.01.T01	AT1G78815	63.566	129	41	3	25	150	38	163	3.07e-55	173
MsG0280007305.01.T01	AT1G16910	58.955	134	49	3	25	155	21	151	3.35e-52	164

Find 48 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTCACTACCTTGATCAATT+TGG	0.137888	2:-13315470	MsG0280007305.01.T01:CDS
AATCCAACTTGTCCTTTCTT+TGG	0.215866	2:-13315431	MsG0280007305.01.T01:CDS
TGTTCCAGTCTCTCCTCTTT+TGG	0.217847	2:+13315573	None:intergenic
ATCCAACTTGTCCTTTCTTT+GGG	0.324334	2:-13315430	MsG0280007305.01.T01:CDS
TTCACTACCTTGATCAATTT+GGG	0.326576	2:-13315469	MsG0280007305.01.T01:CDS
TTGTCCTCTCCGTCAGGCTT+GGG	0.343185	2:-13315381	MsG0280007305.01.T01:CDS
CTTGTCCTCTCCGTCAGGCT+TGG	0.356689	2:-13315382	MsG0280007305.01.T01:CDS
CATGGTGGAAGTAACCCTTT+TGG	0.395005	2:-13315305	MsG0280007305.01.T01:CDS
GCACATGGAGATGGAGGGTT+TGG	0.411549	2:+13315404	None:intergenic
CACTTCCATTGTAGCTGCTA+GGG	0.441671	2:+13315633	None:intergenic
GTCGATGGTTTCTTAAGTAC+TGG	0.444811	2:+13315543	None:intergenic
ATGTGCTTGTCCTCTCCGTC+AGG	0.459665	2:-13315387	MsG0280007305.01.T01:CDS
TGATCAATTTGGGAAGACTA+AGG	0.465045	2:-13315459	MsG0280007305.01.T01:CDS
GTGGTGGCAACAAGATCAAC+AGG	0.470799	2:-13315604	MsG0280007305.01.T01:CDS
CTAACAGCGCCATCACCAAA+AGG	0.486109	2:+13315290	None:intergenic
GAGTTAGCTACCATAAGAAA+AGG	0.502671	2:-13315223	MsG0280007305.01.T01:CDS
TAGTCTTCCCAAATTGATCA+AGG	0.503689	2:+13315462	None:intergenic
GTAGTTGTGATGTTGCGGTT+AGG	0.503903	2:+13315194	None:intergenic
AGAGCCAAAAGAGGAGAGAC+TGG	0.512337	2:-13315577	MsG0280007305.01.T01:CDS
GCTTATGATCAAGAGATTCA+TGG	0.517251	2:-13315323	MsG0280007305.01.T01:CDS
ATTGAAGCAGAAAGTGGTAC+TGG	0.535622	2:+13315521	None:intergenic
TCACTTCCATTGTAGCTGCT+AGG	0.535763	2:+13315632	None:intergenic
TTATACAAGAAATATGGCAT+TGG	0.537262	2:-13315675	None:intergenic
CTTTCTGCTTCAATGTGCAA+TGG	0.545802	2:-13315512	MsG0280007305.01.T01:CDS
TGTCCTCTCCGTCAGGCTTG+GGG	0.552431	2:-13315380	MsG0280007305.01.T01:CDS
GGTAGCCTTGATGCTCTCAT+CGG	0.556787	2:-13315359	MsG0280007305.01.T01:CDS
AGAGGACAAGCACATGGAGA+TGG	0.557220	2:+13315395	None:intergenic
CAAATTGATCAAGGTAGTGA+AGG	0.557585	2:+13315471	None:intergenic
GGGTTTGGCATCCCAAAGAA+AGG	0.560750	2:+13315419	None:intergenic
ACAAACCCTAGCAGCTACAA+TGG	0.562580	2:-13315638	MsG0280007305.01.T01:CDS
TTGCACATTGAAGCAGAAAG+TGG	0.564133	2:+13315515	None:intergenic
TAACAGCGCCATCACCAAAA+GGG	0.569402	2:+13315291	None:intergenic
CATGACAAATAGAAATCAAG+AGG	0.576026	2:+13315160	None:intergenic
GGACAAGCACATGGAGATGG+AGG	0.578694	2:+13315398	None:intergenic
ATCCCAAAGAAAGGACAAGT+TGG	0.587746	2:+13315428	None:intergenic
CAGAAAGTGGTACTGGTCGA+TGG	0.588396	2:+13315528	None:intergenic
GAGAAGTAGTTGTGATGTTG+CGG	0.588969	2:+13315189	None:intergenic
CAAGGCTACCCCAAGCCTGA+CGG	0.597531	2:+13315372	None:intergenic
ACAGGTACGAGAGCCAAAAG+AGG	0.613687	2:-13315586	MsG0280007305.01.T01:CDS
TGACGGAGAGGACAAGCACA+TGG	0.624357	2:+13315389	None:intergenic
AATGGAAGTGATTCAAGTGG+TGG	0.625867	2:-13315620	MsG0280007305.01.T01:CDS
GGCGACCGATGAGAGCATCA+AGG	0.637841	2:+13315354	None:intergenic
GACAAGCACATGGAGATGGA+GGG	0.652537	2:+13315399	None:intergenic
CTACCCCAAGCCTGACGGAG+AGG	0.655871	2:+13315377	None:intergenic
TACAATGGAAGTGATTCAAG+TGG	0.673612	2:-13315623	MsG0280007305.01.T01:CDS
CTTGATCATAAGCAGCGCGG+AGG	0.674942	2:+13315333	None:intergenic
TATGATCAAGAGATTCATGG+TGG	0.715975	2:-13315320	MsG0280007305.01.T01:CDS
TCTCTTGATCATAAGCAGCG+CGG	0.775865	2:+13315330	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TTCACTACCTTGATCAATTT+GGG	-	Chr2:13315368-13315387	MsG0280007305.01.T01:CDS	30.0%
	AATCCAACTTGTCCTTTCTT+TGG	-	Chr2:13315406-13315425	MsG0280007305.01.T01:CDS	35.0%
	ATCCAACTTGTCCTTTCTTT+GGG	-	Chr2:13315407-13315426	MsG0280007305.01.T01:CDS	35.0%
	CAAATTGATCAAGGTAGTGA+AGG	+	Chr2:13315369-13315388	None:intergenic	35.0%
	CTTCACTACCTTGATCAATT+TGG	-	Chr2:13315367-13315386	MsG0280007305.01.T01:CDS	35.0%
	GAGTTAGCTACCATAAGAAA+AGG	-	Chr2:13315614-13315633	MsG0280007305.01.T01:CDS	35.0%
	GCTTATGATCAAGAGATTCA+TGG	-	Chr2:13315514-13315533	MsG0280007305.01.T01:CDS	35.0%
	TACAATGGAAGTGATTCAAG+TGG	-	Chr2:13315214-13315233	MsG0280007305.01.T01:CDS	35.0%
	TAGTCTTCCCAAATTGATCA+AGG	+	Chr2:13315378-13315397	None:intergenic	35.0%
	TATGATCAAGAGATTCATGG+TGG	-	Chr2:13315517-13315536	MsG0280007305.01.T01:CDS	35.0%
	TGATCAATTTGGGAAGACTA+AGG	-	Chr2:13315378-13315397	MsG0280007305.01.T01:CDS	35.0%
!	TAGGTCTCTTCCTTTTCTTA+TGG	+	Chr2:13315627-13315646	None:intergenic	35.0%
!	TGTTCGTGATTTTCAGTCAA+AGG	-	Chr2:13315585-13315604	MsG0280007305.01.T01:CDS	35.0%
	AATGGAAGTGATTCAAGTGG+TGG	-	Chr2:13315217-13315236	MsG0280007305.01.T01:CDS	40.0%
	ATCCCAAAGAAAGGACAAGT+TGG	+	Chr2:13315412-13315431	None:intergenic	40.0%
	ATTGAAGCAGAAAGTGGTAC+TGG	+	Chr2:13315319-13315338	None:intergenic	40.0%
	CTTTCTGCTTCAATGTGCAA+TGG	-	Chr2:13315325-13315344	MsG0280007305.01.T01:CDS	40.0%
	GAGAAGTAGTTGTGATGTTG+CGG	+	Chr2:13315651-13315670	None:intergenic	40.0%
	GTCGATGGTTTCTTAAGTAC+TGG	+	Chr2:13315297-13315316	None:intergenic	40.0%
	TTGCACATTGAAGCAGAAAG+TGG	+	Chr2:13315325-13315344	None:intergenic	40.0%
!	GATTTTCAGTCAAAGGCTAG+AGG	-	Chr2:13315592-13315611	MsG0280007305.01.T01:CDS	40.0%
	ACAAACCCTAGCAGCTACAA+TGG	-	Chr2:13315199-13315218	MsG0280007305.01.T01:CDS	45.0%
	CACTTCCATTGTAGCTGCTA+GGG	+	Chr2:13315207-13315226	None:intergenic	45.0%
	GTAGTTGTGATGTTGCGGTT+AGG	+	Chr2:13315646-13315665	None:intergenic	45.0%
	TAACAGCGCCATCACCAAAA+GGG	+	Chr2:13315549-13315568	None:intergenic	45.0%
	TCACTTCCATTGTAGCTGCT+AGG	+	Chr2:13315208-13315227	None:intergenic	45.0%
	TCTCTTGATCATAAGCAGCG+CGG	+	Chr2:13315510-13315529	None:intergenic	45.0%
!	GGAAGTAACCCTTTTGGTGA+TGG	-	Chr2:13315538-13315557	MsG0280007305.01.T01:CDS	45.0%
!	TGTTCCAGTCTCTCCTCTTT+TGG	+	Chr2:13315267-13315286	None:intergenic	45.0%
!!	CATGGTGGAAGTAACCCTTT+TGG	-	Chr2:13315532-13315551	MsG0280007305.01.T01:CDS	45.0%
	ACAGGTACGAGAGCCAAAAG+AGG	-	Chr2:13315251-13315270	MsG0280007305.01.T01:CDS	50.0%
	AGAGCCAAAAGAGGAGAGAC+TGG	-	Chr2:13315260-13315279	MsG0280007305.01.T01:CDS	50.0%
	AGAGGACAAGCACATGGAGA+TGG	+	Chr2:13315445-13315464	None:intergenic	50.0%
	CTAACAGCGCCATCACCAAA+AGG	+	Chr2:13315550-13315569	None:intergenic	50.0%
	GACAAGCACATGGAGATGGA+GGG	+	Chr2:13315441-13315460	None:intergenic	50.0%
	GGGTTTGGCATCCCAAAGAA+AGG	+	Chr2:13315421-13315440	None:intergenic	50.0%
	GTGGTGGCAACAAGATCAAC+AGG	-	Chr2:13315233-13315252	MsG0280007305.01.T01:CDS	50.0%
!	GGTAGCCTTGATGCTCTCAT+CGG	-	Chr2:13315478-13315497	MsG0280007305.01.T01:CDS	50.0%
!!	CAGAAAGTGGTACTGGTCGA+TGG	+	Chr2:13315312-13315331	None:intergenic	50.0%
!!!	CTTTTGGTGATGGCGCTGTT+AGG	-	Chr2:13315548-13315567	MsG0280007305.01.T01:CDS	50.0%
	ATGTGCTTGTCCTCTCCGTC+AGG	-	Chr2:13315450-13315469	MsG0280007305.01.T01:CDS	55.0%
	CTTGATCATAAGCAGCGCGG+AGG	+	Chr2:13315507-13315526	None:intergenic	55.0%
	GCACATGGAGATGGAGGGTT+TGG	+	Chr2:13315436-13315455	None:intergenic	55.0%
	GGACAAGCACATGGAGATGG+AGG	+	Chr2:13315442-13315461	None:intergenic	55.0%
	TGACGGAGAGGACAAGCACA+TGG	+	Chr2:13315451-13315470	None:intergenic	55.0%
	TTGTCCTCTCCGTCAGGCTT+GGG	-	Chr2:13315456-13315475	MsG0280007305.01.T01:CDS	55.0%
	CAAGGCTACCCCAAGCCTGA+CGG	+	Chr2:13315468-13315487	None:intergenic	60.0%
	CTTGTCCTCTCCGTCAGGCT+TGG	-	Chr2:13315455-13315474	MsG0280007305.01.T01:CDS	60.0%
	TGTCCTCTCCGTCAGGCTTG+GGG	-	Chr2:13315457-13315476	MsG0280007305.01.T01:CDS	60.0%
!!	GGCGACCGATGAGAGCATCA+AGG	+	Chr2:13315486-13315505	None:intergenic	60.0%
	CTACCCCAAGCCTGACGGAG+AGG	+	Chr2:13315463-13315482	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	13315175	13315684	13315175	ID=MsG0280007305.01;Name=MsG0280007305.01
Chr2	mRNA	13315175	13315684	13315175	ID=MsG0280007305.01.T01;Parent=MsG0280007305.01;Name=MsG0280007305.01.T01;_AED=0.38;_eAED=0.38;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|169
Chr2	exon	13315175	13315684	13315175	ID=MsG0280007305.01.T01:exon:2528;Parent=MsG0280007305.01.T01
Chr2	CDS	13315175	13315684	13315175	ID=MsG0280007305.01.T01:cds;Parent=MsG0280007305.01.T01

Gene Sequence

>MsG0280007305.01.T01

ATGGCATTGGTTTCAGCTGAATCAACAAACCCTAGCAGCTACAATGGAAGTGATTCAAGTGGTGGCAACAAGATCAACAGGTACGAGAGCCAAAAGAGGAGAGACTGGAACACATTCTGCCAGTACTTAAGAAACCATCGACCAGTACCACTTTCTGCTTCAATGTGCAATGGAACTCATGTTCTTGAATTCCTTCACTACCTTGATCAATTTGGGAAGACTAAGGTTCACAATCCAACTTGTCCTTTCTTTGGGATGCCAAACCCTCCATCTCCATGTGCTTGTCCTCTCCGTCAGGCTTGGGGTAGCCTTGATGCTCTCATCGGTCGCCTCCGCGCTGCTTATGATCAAGAGATTCATGGTGGAAGTAACCCTTTTGGTGATGGCGCTGTTAGGTTTTACTTGCGTGATGTTCGTGATTTTCAGTCAAAGGCTAGAGGAGTTAGCTACCATAAGAAAAGGAAGAGACCTAACCGCAACATCACAACTACTTCTCAATCATCCTCTTGA

Protein sequence

>MsG0280007305.01.T01

MALVSAESTNPSSYNGSDSSGGNKINRYESQKRRDWNTFCQYLRNHRPVPLSASMCNGTHVLEFLHYLDQFGKTKVHNPTCPFFGMPNPPSPCACPLRQAWGSLDALIGRLRAAYDQEIHGGSNPFGDGAVRFYLRDVRDFQSKARGVSYHKKRKRPNRNITTTSQSSS*