AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001771.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001771.01.T01 MTR_1g442860 96.744 215 6 1 1 215 1 214 1.71e-153 424
MsG0180001771.01.T01 MTR_1g075990 80.460 174 28 2 22 195 16 183 1.28e-90 265
MsG0180001771.01.T01 MTR_5g072510 80.282 142 28 0 55 196 27 168 1.13e-87 258
MsG0180001771.01.T01 MTR_7g097030 75.385 195 47 1 1 195 1 194 3.18e-87 256
MsG0180001771.01.T01 MTR_4g094428 83.704 135 22 0 64 198 45 179 9.49e-87 256
MsG0180001771.01.T01 MTR_1g069825 72.308 195 41 1 1 195 1 182 1.67e-85 253
MsG0180001771.01.T01 MTR_3g031830 70.760 171 39 2 37 196 3 173 4.15e-76 229
MsG0180001771.01.T01 MTR_2g092950 71.724 145 41 0 44 188 2 146 5.32e-75 224
MsG0180001771.01.T01 MTR_4g079930 74.436 133 34 0 56 188 20 152 1.40e-74 223
MsG0180001771.01.T01 MTR_2g076400 65.644 163 55 1 34 195 1 163 1.78e-73 220
MsG0180001771.01.T01 MTR_2g016110 72.662 139 38 0 57 195 27 165 7.82e-72 216
MsG0180001771.01.T01 MTR_7g115700 63.924 158 55 2 40 196 2 158 6.37e-71 214
MsG0180001771.01.T01 MTR_1g080210 78.378 148 32 0 42 189 17 164 6.44e-71 214
MsG0180001771.01.T01 MTR_8g086350 72.800 125 34 0 64 188 30 154 1.01e-57 181
MsG0180001771.01.T01 MTR_1g114020 78.431 51 11 0 58 108 64 114 8.57e-23 90.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001771.01.T01 AT2G31160 84.277 159 25 0 38 196 27 185 6.63e-88 258
MsG0180001771.01.T01 AT1G07090 77.551 147 28 1 51 197 22 163 1.28e-86 254
MsG0180001771.01.T01 AT3G23290 95.238 126 6 0 64 189 47 172 5.96e-82 243
MsG0180001771.01.T01 AT5G58500 80.741 135 25 1 63 196 17 151 9.78e-82 241
MsG0180001771.01.T01 AT5G28490 80.405 148 23 1 47 194 13 154 1.96e-75 226
MsG0180001771.01.T01 AT2G42610 77.344 128 29 0 61 188 21 148 9.93e-73 218
MsG0180001771.01.T01 AT2G42610 77.344 128 29 0 61 188 21 148 9.93e-73 218
MsG0180001771.01.T01 AT3G04510 65.556 180 43 2 15 194 2 162 6.36e-71 215
MsG0180001771.01.T01 AT1G78815 61.745 149 57 0 64 212 39 187 3.54e-66 202
MsG0180001771.01.T01 AT4G18610 64.138 145 51 1 45 188 17 161 5.98e-64 197
MsG0180001771.01.T01 AT1G16910 57.419 155 61 1 39 193 2 151 6.35e-62 191

Find 49 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCGTTACCTCGACCAATT+TGG 0.269006 1:+26666601 MsG0180001771.01.T01:CDS
TGGATTTGGATGTCCATAAA+AGG 0.277270 1:-26666653 None:intergenic
GGACATGGTGCAGGTGGATT+TGG 0.320133 1:-26666667 None:intergenic
GACTTTACCTTCGCGAAGTT+AGG 0.323181 1:+26666806 MsG0180001771.01.T01:CDS
GTTCATCATGTCTTTGTTGT+TGG 0.334448 1:-26666329 None:intergenic
GGTGGTGGTGGTGGCGGCTG+AGG 0.339795 1:-26666886 None:intergenic
GTTGTTGTTGATGTTGTTGA+TGG 0.340470 1:-26666460 None:intergenic
GGGTTTGTTATGTTGATCAT+AGG 0.347871 1:-26666358 None:intergenic
AAGCCAGAAGCTAATCCTTT+TGG 0.361490 1:+26666769 MsG0180001771.01.T01:CDS
GTGGTGGTCTATGGTTTCTT+AGG 0.368488 1:-26666537 None:intergenic
AAATTCTTGAAGAGAATTCA+TGG 0.377394 1:-26666296 None:intergenic
AGACGAGATTGGAACACATT+TGG 0.384522 1:+26666508 MsG0180001771.01.T01:CDS
ACTTTACCTTCGCGAAGTTA+GGG 0.398859 1:+26666807 MsG0180001771.01.T01:CDS
TTATTTGATGGTGGTGGTGG+TGG 0.403399 1:-26666898 None:intergenic
GCACCAAAAGGATTAGCTTC+TGG 0.418214 1:-26666772 None:intergenic
GTTGTAGTTGCATTATTTGA+TGG 0.419514 1:-26666910 None:intergenic
GGTTTGTTATGTTGATCATA+GGG 0.430093 1:-26666357 None:intergenic
GGTAGTCTTGATGCACTCAT+TGG 0.434097 1:+26666712 MsG0180001771.01.T01:CDS
CTTGTCCTCTTCGCCAAGCG+TGG 0.459831 1:+26666689 MsG0180001771.01.T01:CDS
GGTGGTGGTGGCGGCTGAGG+CGG 0.465046 1:-26666883 None:intergenic
AGAATCAAAAGAGACGAGAT+TGG 0.470010 1:+26666497 MsG0180001771.01.T01:CDS
GCATTATTTGATGGTGGTGG+TGG 0.471088 1:-26666901 None:intergenic
TTGTCCTCTTCGCCAAGCGT+GGG 0.488923 1:+26666690 MsG0180001771.01.T01:CDS
CGAGGTAACGCAAGAATTCA+AGG 0.492274 1:-26666591 None:intergenic
AGAGTGAAAGTGGTGGTCTA+TGG 0.493036 1:-26666546 None:intergenic
GTAGTCTTGATGCACTCATT+GGG 0.507513 1:+26666713 MsG0180001771.01.T01:CDS
GTTGCATTATTTGATGGTGG+TGG 0.513644 1:-26666904 None:intergenic
CGAACAGCACGAGCACCAAA+AGG 0.514487 1:-26666784 None:intergenic
GTTGTTGATGTTGTTGATGG+TGG 0.518465 1:-26666457 None:intergenic
CACGCTTGGCGAAGAGGACA+AGG 0.527749 1:-26666688 None:intergenic
CTTTCACTCTCACGTTGTAG+TGG 0.531680 1:+26666559 MsG0180001771.01.T01:CDS
GGATTTGGATGTCCATAAAA+GGG 0.532805 1:-26666652 None:intergenic
TGATGTTGTTGTTGTTGATG+AGG 0.537472 1:-26666434 None:intergenic
GTCATTGACATGGATGATGA+TGG 0.542243 1:-26666379 None:intergenic
CATCAAGACTACCCCACGCT+TGG 0.543485 1:-26666702 None:intergenic
GTAGTTGCATTATTTGATGG+TGG 0.544780 1:-26666907 None:intergenic
AGAGGACAAGGACATGGTGC+AGG 0.565916 1:-26666676 None:intergenic
CTACCCCACGCTTGGCGAAG+AGG 0.567363 1:-26666694 None:intergenic
CTACAACGTGAGAGTGAAAG+TGG 0.580478 1:-26666556 None:intergenic
GGACAAGGACATGGTGCAGG+TGG 0.588264 1:-26666673 None:intergenic
TGTTGTAGATGATGATGGTA+TGG 0.601682 1:-26666401 None:intergenic
GCTGATGTTGTAGATGATGA+TGG 0.601848 1:-26666406 None:intergenic
TGTCCTCTTCGCCAAGCGTG+GGG 0.612011 1:+26666691 MsG0180001771.01.T01:CDS
TGGCGAAGAGGACAAGGACA+TGG 0.629659 1:-26666682 None:intergenic
GATTCTCAAGCTAAAGCTAG+AGG 0.638819 1:+26666829 MsG0180001771.01.T01:CDS
TGATGGTATGGTCATTGACA+TGG 0.640174 1:-26666389 None:intergenic
TCATTGACATGGATGATGAT+GGG 0.643402 1:-26666378 None:intergenic
GAGAATCCCTAACTTCGCGA+AGG 0.649287 1:-26666813 None:intergenic
CAACGTGAGAGTGAAAGTGG+TGG 0.746060 1:-26666553 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GTTGTAGTTGCATTATTTGA+TGG - Chr1:26666913-26666932 None:intergenic 30.0%
!! GGTTTGTTATGTTGATCATA+GGG - Chr1:26666360-26666379 None:intergenic 30.0%
!! TTTTGATTCTCGTATCTACT+TGG - Chr1:26666487-26666506 None:intergenic 30.0%
!!! GAACCTTTGTTTTTCCAAAT+TGG - Chr1:26666618-26666637 None:intergenic 30.0%
AGAATCAAAAGAGACGAGAT+TGG + Chr1:26666497-26666516 MsG0180001771.01.T01:CDS 35.0%
CGACCAATTTGGAAAAACAA+AGG + Chr1:26666612-26666631 MsG0180001771.01.T01:CDS 35.0%
GGATTTGGATGTCCATAAAA+GGG - Chr1:26666655-26666674 None:intergenic 35.0%
GTAGTTGCATTATTTGATGG+TGG - Chr1:26666910-26666929 None:intergenic 35.0%
TCATTGACATGGATGATGAT+GGG - Chr1:26666381-26666400 None:intergenic 35.0%
TGGATTTGGATGTCCATAAA+AGG - Chr1:26666656-26666675 None:intergenic 35.0%
! ACTCAGATTTGTCCCTTTTA+TGG + Chr1:26666640-26666659 MsG0180001771.01.T01:CDS 35.0%
! GTTCATCATGTCTTTGTTGT+TGG - Chr1:26666332-26666351 None:intergenic 35.0%
! GTTGTTGTTGATGTTGTTGA+TGG - Chr1:26666463-26666482 None:intergenic 35.0%
! TGATGTTGTTGTTGTTGATG+AGG - Chr1:26666437-26666456 None:intergenic 35.0%
!! CTGCTTTTGAAGAAAATGGT+GGG + Chr1:26666746-26666765 MsG0180001771.01.T01:CDS 35.0%
!! GGGTTTGTTATGTTGATCAT+AGG - Chr1:26666361-26666380 None:intergenic 35.0%
!! TGTTGTAGATGATGATGGTA+TGG - Chr1:26666404-26666423 None:intergenic 35.0%
!! TTGTTTTTCCAAATTGGTCG+AGG - Chr1:26666612-26666631 None:intergenic 35.0%
!!! AGAGCTGCTTTTGAAGAAAA+TGG + Chr1:26666742-26666761 MsG0180001771.01.T01:CDS 35.0%
ACTTTACCTTCGCGAAGTTA+GGG + Chr1:26666807-26666826 MsG0180001771.01.T01:CDS 40.0%
AGACGAGATTGGAACACATT+TGG + Chr1:26666508-26666527 MsG0180001771.01.T01:CDS 40.0%
GATTCTCAAGCTAAAGCTAG+AGG + Chr1:26666829-26666848 MsG0180001771.01.T01:CDS 40.0%
GCTGATGTTGTAGATGATGA+TGG - Chr1:26666409-26666428 None:intergenic 40.0%
GTCATTGACATGGATGATGA+TGG - Chr1:26666382-26666401 None:intergenic 40.0%
TGATGGTATGGTCATTGACA+TGG - Chr1:26666392-26666411 None:intergenic 40.0%
! AAGCCAGAAGCTAATCCTTT+TGG + Chr1:26666769-26666788 MsG0180001771.01.T01:CDS 40.0%
! GTAGTCTTGATGCACTCATT+GGG + Chr1:26666713-26666732 MsG0180001771.01.T01:CDS 40.0%
! GTTGTTGATGTTGTTGATGG+TGG - Chr1:26666460-26666479 None:intergenic 40.0%
!! GTTGCATTATTTGATGGTGG+TGG - Chr1:26666907-26666926 None:intergenic 40.0%
!!! GCTGCTTTTGAAGAAAATGG+TGG + Chr1:26666745-26666764 MsG0180001771.01.T01:CDS 40.0%
AGAGTGAAAGTGGTGGTCTA+TGG - Chr1:26666549-26666568 None:intergenic 45.0%
CGAGGTAACGCAAGAATTCA+AGG - Chr1:26666594-26666613 None:intergenic 45.0%
CTACAACGTGAGAGTGAAAG+TGG - Chr1:26666559-26666578 None:intergenic 45.0%
CTTTCACTCTCACGTTGTAG+TGG + Chr1:26666559-26666578 MsG0180001771.01.T01:CDS 45.0%
GACTTTACCTTCGCGAAGTT+AGG + Chr1:26666806-26666825 MsG0180001771.01.T01:CDS 45.0%
GTGGTGGTCTATGGTTTCTT+AGG - Chr1:26666540-26666559 None:intergenic 45.0%
TTGCGTTACCTCGACCAATT+TGG + Chr1:26666601-26666620 MsG0180001771.01.T01:CDS 45.0%
! GGTAGTCTTGATGCACTCAT+TGG + Chr1:26666712-26666731 MsG0180001771.01.T01:CDS 45.0%
!! GCACCAAAAGGATTAGCTTC+TGG - Chr1:26666775-26666794 None:intergenic 45.0%
!! GCATTATTTGATGGTGGTGG+TGG - Chr1:26666904-26666923 None:intergenic 45.0%
!! TTATTTGATGGTGGTGGTGG+TGG - Chr1:26666901-26666920 None:intergenic 45.0%
CAACGTGAGAGTGAAAGTGG+TGG - Chr1:26666556-26666575 None:intergenic 50.0%
GAGAATCCCTAACTTCGCGA+AGG - Chr1:26666816-26666835 None:intergenic 50.0%
AGAGGACAAGGACATGGTGC+AGG - Chr1:26666679-26666698 None:intergenic 55.0%
CATCAAGACTACCCCACGCT+TGG - Chr1:26666705-26666724 None:intergenic 55.0%
CGAACAGCACGAGCACCAAA+AGG - Chr1:26666787-26666806 None:intergenic 55.0%
TGGCGAAGAGGACAAGGACA+TGG - Chr1:26666685-26666704 None:intergenic 55.0%
TTGTCCTCTTCGCCAAGCGT+GGG + Chr1:26666690-26666709 MsG0180001771.01.T01:CDS 55.0%
! GGACATGGTGCAGGTGGATT+TGG - Chr1:26666670-26666689 None:intergenic 55.0%
!! TTTGATGGTGGTGGTGGTGG+TGG - Chr1:26666898-26666917 None:intergenic 55.0%
CACGCTTGGCGAAGAGGACA+AGG - Chr1:26666691-26666710 None:intergenic 60.0%
CTTGTCCTCTTCGCCAAGCG+TGG + Chr1:26666689-26666708 MsG0180001771.01.T01:CDS 60.0%
GGACAAGGACATGGTGCAGG+TGG - Chr1:26666676-26666695 None:intergenic 60.0%
TGTCCTCTTCGCCAAGCGTG+GGG + Chr1:26666691-26666710 MsG0180001771.01.T01:CDS 60.0%
CTACCCCACGCTTGGCGAAG+AGG - Chr1:26666697-26666716 None:intergenic 65.0%
!! GATGGTGGTGGTGGTGGTGG+CGG - Chr1:26666895-26666914 None:intergenic 65.0%
!! GGTGGTGGTGGCGGCTGAGG+CGG - Chr1:26666886-26666905 None:intergenic 75.0%
!! GGTGGTGGTGGTGGCGGCTG+AGG - Chr1:26666889-26666908 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr1 gene 26666298 26666945 26666298 ID=MsG0180001771.01;Name=MsG0180001771.01
Chr1 mRNA 26666298 26666945 26666298 ID=MsG0180001771.01.T01;Parent=MsG0180001771.01;Name=MsG0180001771.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|215
Chr1 exon 26666298 26666945 26666298 ID=MsG0180001771.01.T01:exon:35201;Parent=MsG0180001771.01.T01
Chr1 CDS 26666298 26666945 26666298 ID=MsG0180001771.01.T01:cds;Parent=MsG0180001771.01.T01
Gene Sequence

>MsG0180001771.01.T01

ATGAATTCTCTTCAAGAATTTGAATCATCAACCAACAACAAAGACATGATGAACAAAAACCCTATGATCAACATAACAAACCCATCATCATCCATGTCAATGACCATACCATCATCATCTACAACATCAGCATCATCCTCATCAACAACAACAACATCACCACCATCAACAACATCAACAACAACACCAAGTAGATACGAGAATCAAAAGAGACGAGATTGGAACACATTTGGACAATACCTAAGAAACCATAGACCACCACTTTCACTCTCACGTTGTAGTGGAGCTCATGTCCTTGAATTCTTGCGTTACCTCGACCAATTTGGAAAAACAAAGGTTCACACTCAGATTTGTCCCTTTTATGGACATCCAAATCCACCTGCACCATGTCCTTGTCCTCTTCGCCAAGCGTGGGGTAGTCTTGATGCACTCATTGGGAGACTTAGAGCTGCTTTTGAAGAAAATGGTGGGAAGCCAGAAGCTAATCCTTTTGGTGCTCGTGCTGTTCGACTTTACCTTCGCGAAGTTAGGGATTCTCAAGCTAAAGCTAGAGGTATTAGTTATGAGAAAAAGAAGCGCAAGCGTCCGCCTCAGCCGCCACCACCACCACCACCATCAAATAATGCAACTACAACTGATCTTCACTAG

Protein sequence

>MsG0180001771.01.T01

MNSLQEFESSTNNKDMMNKNPMINITNPSSSMSMTIPSSSTTSASSSSTTTTSPPSTTSTTTPSRYENQKRRDWNTFGQYLRNHRPPLSLSRCSGAHVLEFLRYLDQFGKTKVHTQICPFYGHPNPPAPCPCPLRQAWGSLDALIGRLRAAFEENGGKPEANPFGARAVRLYLREVRDSQAKARGISYEKKKRKRPPQPPPPPPPSNNATTTDLH*