AlfalfaGEDB | MsG0580029418.01

M. sativa cultivar ZhongmuNo.1 / MsG0580029418.01

KEGG: -
GO: GO:0003677 GO:0005634
PFAM: PF03790 PF03791

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029418.01.T01	XP_003616932.3	89.894	188	0	1	1	188	1	169	8.65E-117	344

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029418.01.T01	P46608	54.962	131	37	3	59	188	123	232	3.80E-38	137

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029418.01.T01	A5Y4H1	89.894	188	0	1	1	188	1	169	4.13e-117	344

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048357.01	MsG0580029418.01	0.923989	1.280284e-89	5.280104e-85
MsG0180000036.01	MsG0580029418.01	0.805073	1.592147e-49	8.344715e-47
MsG0180000404.01	MsG0580029418.01	0.806651	7.415985e-50	4.046912e-47
MsG0180000632.01	MsG0580029418.01	0.895586	8.329344e-76	8.697515e-72
MsG0180000994.01	MsG0580029418.01	0.807188	5.709593e-50	3.159559e-47
MsG0180001200.01	MsG0580029418.01	0.890678	7.927738e-74	6.734426e-70
MsG0180001771.01	MsG0580029418.01	0.896531	3.372824e-76	3.664363e-72
MsG0180003361.01	MsG0580029418.01	0.805010	1.640471e-49	8.584071e-47
MsG0180003431.01	MsG0580029418.01	0.815049	1.127832e-51	7.693305e-49
MsG0180003741.01	MsG0580029418.01	0.849098	4.021434e-60	7.540722e-57
MsG0180005540.01	MsG0580029418.01	0.800951	1.132325e-48	5.341726e-46
MsG0280010839.01	MsG0580029418.01	0.803930	2.755445e-49	1.402406e-46
MsG0380011496.01	MsG0580029418.01	0.853682	2.033857e-61	4.430547e-58
MsG0380011501.01	MsG0580029418.01	0.837351	5.472611e-57	7.089155e-54
MsG0380011675.01	MsG0580029418.01	0.806307	8.767696e-50	4.742945e-47
MsG0480018909.01	MsG0580029418.01	0.873222	1.737296e-67	7.481799e-64
MsG0480019279.01	MsG0580029418.01	0.831500	1.611674e-55	1.752218e-52
MsG0480020096.01	MsG0580029418.01	0.844560	7.011096e-59	1.136327e-55
MsG0480024022.01	MsG0580029418.01	0.858515	7.815343e-63	1.999739e-59
MsG0580024094.01	MsG0580029418.01	0.828390	9.234011e-55	9.165729e-52
MsG0580025974.01	MsG0580029418.01	0.819022	1.444273e-52	1.098774e-49
MsG0380016465.01	MsG0580029418.01	0.880512	5.163470e-70	2.918900e-66
MsG0380016466.01	MsG0580029418.01	0.829874	4.033089e-55	4.181015e-52
MsG0380017823.01	MsG0580029418.01	0.835712	1.430041e-56	1.763251e-53
MsG0380017869.01	MsG0580029418.01	0.830658	2.594766e-55	2.751463e-52
MsG0580026198.01	MsG0580029418.01	0.805014	1.637410e-49	8.568946e-47
MsG0580027925.01	MsG0580029418.01	0.836443	9.331277e-57	1.176152e-53
MsG0580029310.01	MsG0580029418.01	0.863822	1.892129e-64	5.813461e-61
MsG0580029405.01	MsG0580029418.01	0.854324	1.327811e-61	2.954161e-58
MsG0580029406.01	MsG0580029418.01	0.835389	1.726344e-56	2.108079e-53
MsG0580029417.01	MsG0580029418.01	0.932525	7.473487e-95	5.031375e-90
MsG0580029418.01	MsG0680031342.01	0.837169	6.090074e-57	7.845671e-54
MsG0580029418.01	MsG0680031428.01	0.805977	1.028601e-49	5.517900e-47
MsG0580029418.01	MsG0680031707.01	0.935584	6.733729e-97	5.464042e-92
MsG0580029418.01	MsG0680031959.01	0.806238	9.063911e-50	4.894830e-47
MsG0580029418.01	MsG0680033143.01	0.838502	2.768809e-57	3.715646e-54
MsG0580029418.01	MsG0680033933.01	0.802605	5.182935e-49	2.549637e-46
MsG0580029418.01	MsG0680034835.01	0.809101	2.234434e-50	1.300194e-47
MsG0580029418.01	MsG0680035658.01	0.811962	5.384687e-51	3.379788e-48
MsG0580029418.01	MsG0680035683.01	0.823542	1.309460e-53	1.130760e-50
MsG0580029418.01	MsG0780036148.01	0.833336	5.653478e-56	6.491565e-53
MsG0580029418.01	MsG0780036178.01	0.809945	1.471899e-50	8.758762e-48
MsG0580029418.01	MsG0780038048.01	0.809940	1.475991e-50	8.781774e-48
MsG0580029418.01	MsG0780040432.01	0.819150	1.350704e-52	1.031072e-49
MsG0580029418.01	MsG0780040846.01	0.877850	4.512513e-69	2.303896e-65
MsG0580029418.01	MsG0780040966.01	0.889229	2.920001e-73	2.335573e-69
MsG0580029418.01	MsG0780041259.01	0.839207	1.819601e-57	2.495068e-54
MsG0580029418.01	MsG0780041676.01	0.847526	1.094056e-59	1.950273e-56
MsG0580029418.01	MsG0780041698.01	0.837608	4.700245e-57	6.138009e-54
MsG0580029418.01	MsG0880041858.01	0.903105	4.892646e-79	7.137672e-75
MsG0580029418.01	MsG0880042442.01	0.858840	6.250540e-63	1.617224e-59
MsG0580029418.01	MsG0880042958.01	0.840895	6.601741e-58	9.538215e-55
MsG0580029418.01	MsG0880042959.01	0.842800	2.072261e-58	3.177551e-55
MsG0580029418.01	MsG0880045585.01	0.844273	8.373278e-59	1.344808e-55
MsG0580029418.01	MsG0880045586.01	0.863661	2.122630e-64	6.485783e-61
MsG0580029418.01	MsG0880046636.01	0.803927	2.759024e-49	1.404114e-46
MsG0280007252.01	MsG0580029418.01	0.845210	4.680026e-59	7.742558e-56
MsG0280007305.01	MsG0580029418.01	0.953309	3.639426e-111	9.475992e-106
MsG0280007414.01	MsG0580029418.01	0.908557	1.501165e-81	2.799509e-77
MsG0280008390.01	MsG0580029418.01	0.823733	1.181807e-53	1.026255e-50
MsG0280008391.01	MsG0580029418.01	0.827719	1.339354e-54	1.303634e-51
MsG0280008392.01	MsG0580029418.01	0.835362	1.754294e-56	2.140545e-53
MsG0280008395.01	MsG0580029418.01	0.844749	6.234080e-59	1.016384e-55
MsG0280008397.01	MsG0580029418.01	0.806717	7.182787e-50	3.926524e-47
MsG0280010163.01	MsG0580029418.01	0.820540	6.498970e-53	5.156881e-50
MsG0280010320.01	MsG0580029418.01	0.835363	1.753184e-56	2.139275e-53

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029418.01.T01	MTR_5g085860	89.080	174	0	1	15	188	1	155	4.60e-110	317
MsG0580029418.01.T01	MTR_2g024390	53.968	126	35	2	60	185	117	219	1.90e-36	130
MsG0580029418.01.T01	MTR_5g033720	40.945	127	52	2	52	178	64	167	1.62e-26	103
MsG0580029418.01.T01	MTR_1g017080	39.844	128	54	2	54	181	113	217	5.47e-23	94.7
MsG0580029418.01.T01	MTR_1g084060	38.168	131	54	2	55	185	14	117	7.14e-21	87.4
MsG0580029418.01.T01	MTR_1g084060	38.168	131	54	2	55	185	14	117	1.10e-20	87.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029418.01.T01	AT1G62360	55.833	120	30	2	60	179	121	217	1.35e-34	125
MsG0580029418.01.T01	AT1G23380	37.692	130	60	2	50	179	75	183	1.02e-23	95.9
MsG0580029418.01.T01	AT4G08150	43.103	116	44	2	63	178	137	230	2.05e-23	96.3
MsG0580029418.01.T01	AT1G23380	36.923	130	59	2	50	179	75	181	6.62e-23	94.0
MsG0580029418.01.T01	AT1G70510	36.364	143	60	3	52	186	61	180	3.33e-22	91.7
MsG0580029418.01.T01	AT1G70510	35.374	147	60	4	52	186	61	184	4.16e-20	86.3

Find 39 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGCAGTGAGATTTCTTCTA+TGG	0.262136	5:-96763631	MsG0580029418.01.T01:CDS
TGTTGTTGTTGGTGTTGTTC+TGG	0.309721	5:+96763502	None:intergenic
GAAATATTGAATGGCTCTTC+AGG	0.317305	5:-96763049	MsG0580029418.01.T01:CDS
CCTCTGAGTAACAATTCTTC+TGG	0.333607	5:-96763555	MsG0580029418.01.T01:CDS
CTCTTACCTCAATTGCTTGA+AGG	0.355056	5:-96763430	MsG0580029418.01.T01:CDS
TGATGAACATGGAAACTAAT+AGG	0.357473	5:-96763593	MsG0580029418.01.T01:CDS
TCTAAGCTAGCCACCACTTC+AGG	0.359706	5:+96763091	None:intergenic
TTTGAAACTATGTTAGGGTT+TGG	0.362347	5:-96763663	None:intergenic
TCCAGGTTTGGATCAGTTCA+TGG	0.403203	5:-96762990	MsG0580029418.01.T01:CDS
TGAAGTGGTGGCTAGCTTAG+AGG	0.404107	5:-96763089	MsG0580029418.01.T01:CDS
GAAAAGCATGGCTTCTTTGA+AGG	0.406625	5:+96762919	None:intergenic
ATCCCTACAAGTGTTGTTGT+TGG	0.410423	5:+96763491	None:intergenic
ACACCAACAACAACACTTGT+AGG	0.415678	5:-96763494	MsG0580029418.01.T01:CDS
TGTGTGAGAGTTTGAAGTTC+AGG	0.423139	5:+96763406	None:intergenic
TATAGGTGAAGATCCAGGTT+TGG	0.460482	5:-96763002	MsG0580029418.01.T01:CDS
GAAACTATGTTAGGGTTTGG+AGG	0.466056	5:-96763660	None:intergenic
GGAAGTAGTAGTTCTTGTAT+AGG	0.474614	5:-96763019	MsG0580029418.01.T01:CDS
TCAGGACCTTCAAGCAATTG+AGG	0.486076	5:+96763424	None:intergenic
GTTGCTGTTTCAACTGACTT+TGG	0.488737	5:-96762866	MsG0580029418.01.T01:CDS
AAAAGCATGGCTTCTTTGAA+GGG	0.505700	5:+96762920	None:intergenic
GCTGAGACTCAATTCTTGAA+AGG	0.524479	5:+96762897	None:intergenic
TCCATGAACTGATCCAAACC+TGG	0.528067	5:+96762989	None:intergenic
CACTTGTAGGGATAAAATCA+TGG	0.535342	5:-96763481	MsG0580029418.01.T01:CDS
CTTCAAGCAATTGAGGTAAG+AGG	0.538407	5:+96763431	None:intergenic
TCTTGTATAGGTGAAGATCC+AGG	0.538996	5:-96763007	MsG0580029418.01.T01:CDS
AATGGCTCTTCAGGAAGGAC+AGG	0.539828	5:-96763040	MsG0580029418.01.T01:CDS
GACAAGAGACGAGGAAAGAG+AGG	0.541929	5:+96763453	None:intergenic
AAGAATTGAGTCTCAGCTCA+AGG	0.557965	5:-96762891	MsG0580029418.01.T01:CDS
CACCAACAACAACACTTGTA+GGG	0.569598	5:-96763493	MsG0580029418.01.T01:CDS
AATTCTTGAAAGGAAAAGCA+TGG	0.577216	5:+96762907	None:intergenic
GGTTGGAGCACCTCCTGAAG+TGG	0.595718	5:-96763104	MsG0580029418.01.T01:CDS
GCAAAATGTGAAATATTGAA+TGG	0.601951	5:-96763058	MsG0580029418.01.T01:CDS
TCAAAATCAAATGATGAACA+TGG	0.604915	5:-96763604	MsG0580029418.01.T01:CDS
TGGAGCACCTCCTGAAGTGG+TGG	0.608141	5:-96763101	MsG0580029418.01.T01:CDS
TATTGAATGGCTCTTCAGGA+AGG	0.613053	5:-96763045	MsG0580029418.01.T01:CDS
AAGCTAGCCACCACTTCAGG+AGG	0.647427	5:+96763094	None:intergenic
CCAGAAGAATTGTTACTCAG+AGG	0.660627	5:+96763555	None:intergenic
AGGTAAGAGGACAAGAGACG+AGG	0.679022	5:+96763444	None:intergenic
AGGTTTGGATCAGTTCATGG+AGG	0.735955	5:-96762987	MsG0580029418.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AGATTTTTTTTTAAACTTTA+TGG	-	Chr5:96763326-96763345	MsG0580029418.01.T01:intron	10.0%
!	GCAAAATGTGAAATATTGAA+TGG	-	Chr5:96763449-96763468	MsG0580029418.01.T01:CDS	25.0%
!	TCAAAATCAAATGATGAACA+TGG	-	Chr5:96762903-96762922	MsG0580029418.01.T01:CDS	25.0%
!!	TCTCAAAAACAAACATTTTG+TGG	+	Chr5:96763309-96763328	None:intergenic	25.0%
!!!	TTTAAGTGTGATTTTTTTGC+AGG	-	Chr5:96763382-96763401	MsG0580029418.01.T01:intron	25.0%
	AATTCTTGAAAGGAAAAGCA+TGG	+	Chr5:96763603-96763622	None:intergenic	30.0%
	TGATGAACATGGAAACTAAT+AGG	-	Chr5:96762914-96762933	MsG0580029418.01.T01:CDS	30.0%
	CACTTGTAGGGATAAAATCA+TGG	-	Chr5:96763026-96763045	MsG0580029418.01.T01:CDS	35.0%
	GAAATATTGAATGGCTCTTC+AGG	-	Chr5:96763458-96763477	MsG0580029418.01.T01:CDS	35.0%
	GGAAGTAGTAGTTCTTGTAT+AGG	-	Chr5:96763488-96763507	MsG0580029418.01.T01:CDS	35.0%
	TTGCAGTGAGATTTCTTCTA+TGG	-	Chr5:96762876-96762895	MsG0580029418.01.T01:CDS	35.0%
!	AAAAGCATGGCTTCTTTGAA+GGG	+	Chr5:96763590-96763609	None:intergenic	35.0%
!!!	AGTGTGATTTTTTTGCAGGT+TGG	-	Chr5:96763386-96763405	MsG0580029418.01.T01:intron	35.0%
!!!	CAAGTTTTCGCTTGTTTTGA+TGG	-	Chr5:96763359-96763378	MsG0580029418.01.T01:intron	35.0%
	AAGAATTGAGTCTCAGCTCA+AGG	-	Chr5:96763616-96763635	MsG0580029418.01.T01:CDS	40.0%
	ACACCAACAACAACACTTGT+AGG	-	Chr5:96763013-96763032	MsG0580029418.01.T01:CDS	40.0%
	CACCAACAACAACACTTGTA+GGG	-	Chr5:96763014-96763033	MsG0580029418.01.T01:CDS	40.0%
	CCAGAAGAATTGTTACTCAG+AGG	+	Chr5:96762955-96762974	None:intergenic	40.0%
	CCTCTGAGTAACAATTCTTC+TGG	-	Chr5:96762952-96762971	MsG0580029418.01.T01:CDS	40.0%
	CTCTTACCTCAATTGCTTGA+AGG	-	Chr5:96763077-96763096	MsG0580029418.01.T01:CDS	40.0%
	CTTCAAGCAATTGAGGTAAG+AGG	+	Chr5:96763079-96763098	None:intergenic	40.0%
	GCTGAGACTCAATTCTTGAA+AGG	+	Chr5:96763613-96763632	None:intergenic	40.0%
	TATAGGTGAAGATCCAGGTT+TGG	-	Chr5:96763505-96763524	MsG0580029418.01.T01:CDS	40.0%
	TATTGAATGGCTCTTCAGGA+AGG	-	Chr5:96763462-96763481	MsG0580029418.01.T01:CDS	40.0%
	TCTTGTATAGGTGAAGATCC+AGG	-	Chr5:96763500-96763519	MsG0580029418.01.T01:CDS	40.0%
!	ATCCCTACAAGTGTTGTTGT+TGG	+	Chr5:96763019-96763038	None:intergenic	40.0%
!	GAAAAGCATGGCTTCTTTGA+AGG	+	Chr5:96763591-96763610	None:intergenic	40.0%
!	TGTGTGAGAGTTTGAAGTTC+AGG	+	Chr5:96763104-96763123	None:intergenic	40.0%
!!	GTTGCTGTTTCAACTGACTT+TGG	-	Chr5:96763641-96763660	MsG0580029418.01.T01:CDS	40.0%
!!	TGTTGTTGTTGGTGTTGTTC+TGG	+	Chr5:96763008-96763027	None:intergenic	40.0%
	AGGTTTGGATCAGTTCATGG+AGG	-	Chr5:96763520-96763539	MsG0580029418.01.T01:CDS	45.0%
	TCAGGACCTTCAAGCAATTG+AGG	+	Chr5:96763086-96763105	None:intergenic	45.0%
	TCCAGGTTTGGATCAGTTCA+TGG	-	Chr5:96763517-96763536	MsG0580029418.01.T01:CDS	45.0%
	TCCATGAACTGATCCAAACC+TGG	+	Chr5:96763521-96763540	None:intergenic	45.0%
	AATGGCTCTTCAGGAAGGAC+AGG	-	Chr5:96763467-96763486	MsG0580029418.01.T01:CDS	50.0%
	AGGTAAGAGGACAAGAGACG+AGG	+	Chr5:96763066-96763085	None:intergenic	50.0%
	GACAAGAGACGAGGAAAGAG+AGG	+	Chr5:96763057-96763076	None:intergenic	50.0%
	TCTAAGCTAGCCACCACTTC+AGG	+	Chr5:96763419-96763438	None:intergenic	50.0%
!!	TGAAGTGGTGGCTAGCTTAG+AGG	-	Chr5:96763418-96763437	MsG0580029418.01.T01:CDS	50.0%
	AAGCTAGCCACCACTTCAGG+AGG	+	Chr5:96763416-96763435	None:intergenic	55.0%
!	GGTTGGAGCACCTCCTGAAG+TGG	-	Chr5:96763403-96763422	MsG0580029418.01.T01:CDS	60.0%
!	TGGAGCACCTCCTGAAGTGG+TGG	-	Chr5:96763406-96763425	MsG0580029418.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	96762853	96763676	96762853	ID=MsG0580029418.01;Name=MsG0580029418.01
Chr5	mRNA	96762853	96763676	96762853	ID=MsG0580029418.01.T01;Parent=MsG0580029418.01;Name=MsG0580029418.01.T01;_AED=0.48;_eAED=0.53;_QI=0\|0\|0\|1\|0\|0\|2\|0\|191
Chr5	exon	96763390	96763676	96763390	ID=MsG0580029418.01.T01:exon:24673;Parent=MsG0580029418.01.T01
Chr5	exon	96762853	96763141	96762853	ID=MsG0580029418.01.T01:exon:24672;Parent=MsG0580029418.01.T01
Chr5	CDS	96763390	96763676	96763390	ID=MsG0580029418.01.T01:cds;Parent=MsG0580029418.01.T01
Chr5	CDS	96762853	96763141	96762853	ID=MsG0580029418.01.T01:cds;Parent=MsG0580029418.01.T01

Gene Sequence

>MsG0580029418.01.T01

ATGTTAGGGTTTGGAGGAAACAGTTGCAGTGAGATTTCTTCTATGGATCATCAAAATCAAATGATGAACATGGAAACTAATAGGAAGTTTTTTTCATTTCCTCTGAGTAACAATTCTTCTGGTGTTCAAAATCATCATAACTACACTCAACACCAGAACAACACCAACAACAACACTTGTAGGGATAAAATCATGGCTCATCCTCTCTTTCCTCGTCTCTTGTCCTCTTACCTCAATTGCTTGAAGGTCCTGAACTTCAAACTCTCACACACACTTAGCTTTTTCTTTGTGATTTTTTTGCAGGTTGGAGCACCTCCTGAAGTGGTGGCTAGCTTAGAGGAGTCATGTGCAAAATGTGAAATATTGAATGGCTCTTCAGGAAGGACAGGAAGTAGTAGTTCTTGTATAGGTGAAGATCCAGGTTTGGATCAGTTCATGGAGGCATATTGTGAGATGCTTATCAAGTATGAGCAAGAACTCACTAAACCCTTCAAAGAAGCCATGCTTTTCCTTTCAAGAATTGAGTCTCAGCTCAAGGCTGTTGCTGTTTCAACTGACTTTGGTGATTACCCTTAA

Protein sequence

>MsG0580029418.01.T01

MLGFGGNSCSEISSMDHQNQMMNMETNRKFFSFPLSNNSSGVQNHHNYTQHQNNTNNNTCRDKIMAHPLFPRLLSSYLNCLKVLNFKLSHTLSFFFVIFLQVGAPPEVVASLEESCAKCEILNGSSGRTGSSSSCIGEDPGLDQFMEAYCEMLIKYEQELTKPFKEAMLFLSRIESQLKAVAVSTDFGDYP*