AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007500.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007500.01.T01 MTR_2g026590 91.087 561 21 2 1 545 1 548 0.0 970
MsG0280007500.01.T01 MTR_4g133890 63.351 573 157 6 6 546 19 570 0.0 638
MsG0280007500.01.T01 MTR_4g133890 63.287 572 157 6 6 545 11 561 0.0 637
MsG0280007500.01.T01 MTR_8g105350 44.991 549 242 7 45 556 76 601 5.93e-137 413
MsG0280007500.01.T01 MTR_1g032520 45.421 546 244 9 45 556 71 596 2.56e-136 411
MsG0280007500.01.T01 MTR_8g021120 47.925 530 224 10 45 542 72 581 1.33e-133 404
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007500.01.T01 AT4G17220 54.473 503 167 7 45 526 25 486 1.78e-155 457
MsG0280007500.01.T01 AT1G24764 46.604 530 235 8 45 542 93 606 1.62e-141 426
MsG0280007500.01.T01 AT1G68060 48.302 530 222 12 45 540 85 596 5.27e-141 424
MsG0280007500.01.T01 AT1G24764 46.604 530 235 8 45 542 152 665 3.06e-140 424
MsG0280007500.01.T01 AT2G01750 45.607 535 244 7 45 548 92 610 2.90e-136 412
MsG0280007500.01.T01 AT2G01750 45.607 535 244 7 45 548 92 610 3.09e-136 412
MsG0280007500.01.T01 AT1G14840 46.455 536 229 13 45 546 68 579 3.11e-134 406
MsG0280007500.01.T01 AT1G24764 46.269 469 204 8 106 542 1 453 8.79e-123 372
MsG0280007500.01.T01 AT1G14840 44.216 536 226 14 45 546 68 564 3.58e-119 367

Find 87 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGTCAGTTTCTTGATTTCT+AGG 0.120680 2:-16344568 None:intergenic
CTTAGCTTCAGTTGCCCTTA+TGG 0.199525 2:-16344241 None:intergenic
ATTCTTTATGAAGAGAAAAT+TGG 0.212662 2:+16346705 MsG0280007500.01.T01:CDS
GGTAGAACCATATTTCATTT+TGG 0.221595 2:-16345932 None:intergenic
TACCTTAATTTCTTCATCTT+TGG 0.231823 2:-16346199 None:intergenic
TTCCAAAAGCCAAACATAAA+AGG 0.248082 2:+16345865 MsG0280007500.01.T01:CDS
GAAGTTCAAACTTCTCTAAA+AGG 0.264788 2:+16346577 MsG0280007500.01.T01:CDS
TCTTCTAACCATTGCCTAAC+AGG 0.277147 2:-16345486 None:intergenic
CACCAAAGATGAAGAAATTA+AGG 0.291999 2:+16346197 MsG0280007500.01.T01:CDS
ATATTCGATTTATCAGCTTT+TGG 0.300457 2:-16345760 None:intergenic
AGCATAACCAAAATGAAATA+TGG 0.322617 2:+16345925 MsG0280007500.01.T01:CDS
ATTTACCAGAAAGAAGAAAA+TGG 0.347654 2:+16343515 MsG0280007500.01.T01:intron
TCTACAGATTACAGCACTTC+AGG 0.391041 2:+16344836 MsG0280007500.01.T01:intron
GAAAGCACTGATGTGGTCTC+TGG 0.402489 2:+16346099 MsG0280007500.01.T01:CDS
AGGTTAACTATAGAAGTAAA+AGG 0.407721 2:-16346636 None:intergenic
GTGATAAGGTCATGCCTGTT+AGG 0.415652 2:+16345472 MsG0280007500.01.T01:CDS
TCAGGAAGATAAGAAAGCTT+TGG 0.418519 2:+16344854 MsG0280007500.01.T01:CDS
ACAATGGTGGATTGAGAAAC+AGG 0.418721 2:+16345800 MsG0280007500.01.T01:CDS
AAAAGGCAACATAGACAGTA+TGG 0.427289 2:+16345882 MsG0280007500.01.T01:CDS
ATCTCCAACACAACAACATC+TGG 0.433211 2:-16343567 None:intergenic
CCTCAAGTTCATGAATCGTT+TGG 0.434053 2:-16345169 None:intergenic
AAAAGCAGAATCACAATTGA+AGG 0.437230 2:+16345575 MsG0280007500.01.T01:CDS
AAGATTACACACAGATCAAA+AGG 0.452964 2:+16344643 MsG0280007500.01.T01:CDS
TTAGTATAATTTCAGTGGTA+AGG 0.457236 2:+16343768 MsG0280007500.01.T01:intron
GAGAAATGAAGTAAGTAAGA+TGG 0.460243 2:+16344436 MsG0280007500.01.T01:CDS
ATGGTGAAATCAAGGCCATA+AGG 0.461955 2:+16344226 MsG0280007500.01.T01:CDS
AGAAGGCTTAAAGCAATTCT+CGG 0.473398 2:+16345711 MsG0280007500.01.T01:CDS
GAGGAGCTCATCTTATCAAA+TGG 0.475247 2:+16345188 MsG0280007500.01.T01:CDS
CGCAACAGTTGCAATTCTGT+TGG 0.479391 2:-16345427 None:intergenic
CATCAATAAAATCCGATGAT+AGG 0.480394 2:+16346544 MsG0280007500.01.T01:CDS
CTTTGCAAAACAAAACTCAT+AGG 0.480956 2:+16343595 MsG0280007500.01.T01:CDS
CAATGCTATAAAATTACTGC+AGG 0.481570 2:+16346662 MsG0280007500.01.T01:CDS
TTTAGAGTCTTCATCAGCAT+TGG 0.483845 2:-16346055 None:intergenic
TGGAGATACTGAAAATGCAT+TGG 0.489587 2:-16345740 None:intergenic
TCTCAGACTCTGTAAGTTAG+TGG 0.498944 2:-16346810 None:intergenic
GGCAACATAGACAGTATGGC+TGG 0.502574 2:+16345886 MsG0280007500.01.T01:CDS
TTTCCAACGGTAGATCCTCT+TGG 0.502837 2:-16345835 None:intergenic
TGATGCATTGACAAAGGCTA+TGG 0.508671 2:+16346467 MsG0280007500.01.T01:CDS
ATGGAAAGATGAAAGTGATA+AGG 0.511209 2:+16345458 MsG0280007500.01.T01:CDS
CAAAGGCTATGGAAGTAGAA+TGG 0.511214 2:+16346478 MsG0280007500.01.T01:CDS
CTTTATGAAGAGAAAATTGG+AGG 0.512076 2:+16346708 MsG0280007500.01.T01:CDS
CAACTGTTGCGGCTAATGAA+TGG 0.512914 2:+16345439 MsG0280007500.01.T01:CDS
AACATGCCATGGTGAAATCA+AGG 0.522834 2:+16344218 MsG0280007500.01.T01:CDS
TGCGAGACTAAGACTTAAAG+AGG 0.531452 2:+16344460 MsG0280007500.01.T01:CDS
GGCGTGATGACACTTTCAAA+TGG 0.532270 2:-16344680 None:intergenic
ACAACATCTGGTTTGACACT+AGG 0.538398 2:-16343555 None:intergenic
TAAAATTTCAGGAGGAGAAG+AGG 0.539106 2:+16345367 MsG0280007500.01.T01:intron
AAGCCACCGGCAAAAGATTG+TGG 0.540851 2:+16345130 MsG0280007500.01.T01:CDS
AGAAGAAAGAAAGATTATGC+AGG 0.548152 2:+16345503 MsG0280007500.01.T01:CDS
CAAACCAGATGTTGTTGTGT+TGG 0.552026 2:+16343563 MsG0280007500.01.T01:CDS
AGATCTCAGGGAATGAAGAA+AGG 0.554624 2:+16343112 None:intergenic
CACTAGAGAGGGAATTAGCA+AGG 0.557388 2:+16345391 MsG0280007500.01.T01:CDS
CAACAGAATTGCAACTGTTG+CGG 0.557551 2:+16345428 MsG0280007500.01.T01:CDS
TTAAGTTTCGTAACGAACAA+TGG 0.569896 2:+16345784 MsG0280007500.01.T01:CDS
CAATAAAATCCGATGATAGG+AGG 0.573485 2:+16346547 MsG0280007500.01.T01:CDS
TGGTGAAATCAAGGCCATAA+GGG 0.577785 2:+16344227 MsG0280007500.01.T01:CDS
ATCTTAATCTCGTCTTCAAG+AGG 0.579230 2:-16344701 None:intergenic
GAAAATGGTTGTCAATGAAG+AGG 0.581609 2:+16343530 MsG0280007500.01.T01:exon
AGACGAAAAGAAAGATGCGT+TGG 0.582689 2:+16344589 MsG0280007500.01.T01:CDS
AACTGAAGCTAAGAAAGATA+AGG 0.586496 2:+16344251 MsG0280007500.01.T01:CDS
GCTCACAAAGTCGAAAGAGT+TGG 0.587191 2:+16344881 MsG0280007500.01.T01:CDS
GAAAGATAAGGCCATAGAAG+AGG 0.588351 2:+16344263 MsG0280007500.01.T01:CDS
TTATGCAGGCAGAGATGCAA+AGG 0.590511 2:+16345517 MsG0280007500.01.T01:CDS
AGGAGAAGAGGACACTAGAG+AGG 0.591941 2:+16345379 MsG0280007500.01.T01:CDS
CGCAGGATAAACTGAAGCTG+AGG 0.593606 2:+16345674 MsG0280007500.01.T01:intron
CCAAACGATTCATGAACTTG+AGG 0.595747 2:+16345169 MsG0280007500.01.T01:CDS
AATGAACTTGCAACATGCCA+TGG 0.604754 2:+16344207 MsG0280007500.01.T01:CDS
GAAAGTTGATGCATTGACAA+AGG 0.609150 2:+16346461 MsG0280007500.01.T01:CDS
AGGTCATGCCTGTTAGGCAA+TGG 0.612732 2:+16345478 MsG0280007500.01.T01:CDS
TTATGGCCTTGATTTCACCA+TGG 0.621711 2:-16344224 None:intergenic
AAGATAAGAAAGCTTTGGAA+CGG 0.627349 2:+16344859 MsG0280007500.01.T01:CDS
TGATTTAGAAAGCACTGATG+TGG 0.627887 2:+16346092 MsG0280007500.01.T01:CDS
AGTTTCGTAACGAACAATGG+TGG 0.630214 2:+16345787 MsG0280007500.01.T01:CDS
CAACCAAGAGGATCTACCGT+TGG 0.630663 2:+16345832 MsG0280007500.01.T01:CDS
GTAATCACGAATGAGATTAG+CGG 0.635687 2:-16345216 None:intergenic
GCAACATAGACAGTATGGCT+GGG 0.648667 2:+16345887 MsG0280007500.01.T01:CDS
TGGCATTGCTTGAAGCAGAG+AGG 0.652208 2:+16344901 MsG0280007500.01.T01:CDS
ATCACGAATGAGATTAGCGG+TGG 0.652339 2:-16345213 None:intergenic
TTGGAACAAAGTGTTTCCAA+CGG 0.656799 2:-16345848 None:intergenic
GGAGAAGAGGACACTAGAGA+GGG 0.660251 2:+16345380 MsG0280007500.01.T01:CDS
AATGCTATAAAATTACTGCA+GGG 0.668930 2:+16346663 MsG0280007500.01.T01:CDS
AAATCTTAGAGAAAAGCCAC+CGG 0.668990 2:+16345117 MsG0280007500.01.T01:CDS
AATGAGATACAGGTGAACAC+AGG 0.676366 2:+16346021 MsG0280007500.01.T01:CDS
ACTAGAGAGGGAATTAGCAA+GGG 0.681716 2:+16345392 MsG0280007500.01.T01:CDS
GATTAAGATGTATAGAACTG+AGG 0.694125 2:+16344715 MsG0280007500.01.T01:CDS
AGGTCTACATCACAACCAAG+AGG 0.700161 2:+16345820 MsG0280007500.01.T01:CDS
TGATTCTGCAGCATATTGTG+CGG 0.724358 2:-16344612 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATATTATTATTCCAT+AGG - Chr2:16343464-16343483 None:intergenic 10.0%
!!! AAAAGGTATTTAAAAAATAA+AGG + Chr2:16343869-16343888 MsG0280007500.01.T01:intron 10.0%
!!! TAATTTATTTTTTTCTATGT+AGG + Chr2:16345598-16345617 MsG0280007500.01.T01:intron 10.0%
!! TAAAAAAATCAATAGATCAA+GGG + Chr2:16344049-16344068 MsG0280007500.01.T01:intron 15.0%
!! TTAAAAAAATCAATAGATCA+AGG + Chr2:16344048-16344067 MsG0280007500.01.T01:intron 15.0%
!! AAACAAATATGTTCTAACTT+TGG - Chr2:16344343-16344362 None:intergenic 20.0%
!! AGGAAAAATACTTATATAGT+AGG + Chr2:16343383-16343402 MsG0280007500.01.T01:intron 20.0%
!! ATTCTTTATGAAGAGAAAAT+TGG + Chr2:16346705-16346724 MsG0280007500.01.T01:CDS 20.0%
!! GTTCAAAGTACAAAAAATAT+GGG + Chr2:16343256-16343275 MsG0280007500.01.T01:intron 20.0%
!! TTCAAAGTACAAAAAATATG+GGG + Chr2:16343257-16343276 MsG0280007500.01.T01:intron 20.0%
!!! CATAGAATTTATCATTGTTT+TGG - Chr2:16344515-16344534 None:intergenic 20.0%
!!! CTATATAAGTATTTTTCCTT+TGG - Chr2:16343382-16343401 None:intergenic 20.0%
!!! CTATTTTTACTCCTTATTTT+TGG + Chr2:16343738-16343757 MsG0280007500.01.T01:intron 20.0%
!!! TTCTTTTAACACATTAACAA+TGG - Chr2:16343914-16343933 None:intergenic 20.0%
!!! TTTAGTTCTATGTAACAAAA+GGG - Chr2:16346409-16346428 None:intergenic 20.0%
! AAAAACAAATACCTCTTCTA+TGG - Chr2:16344277-16344296 None:intergenic 25.0%
! AAAAAGAAGTCATCAATTTG+AGG + Chr2:16346142-16346161 MsG0280007500.01.T01:CDS 25.0%
! AACTAGTGACAATTTATTCA+TGG + Chr2:16343994-16344013 MsG0280007500.01.T01:intron 25.0%
! AATGCTATAAAATTACTGCA+GGG + Chr2:16346663-16346682 MsG0280007500.01.T01:CDS 25.0%
! AGCATAACCAAAATGAAATA+TGG + Chr2:16345925-16345944 MsG0280007500.01.T01:CDS 25.0%
! AGGTTAACTATAGAAGTAAA+AGG - Chr2:16346639-16346658 None:intergenic 25.0%
! ATTTACCAGAAAGAAGAAAA+TGG + Chr2:16343515-16343534 MsG0280007500.01.T01:intron 25.0%
! ATTTGCTTCATCATTTGATT+AGG + Chr2:16343647-16343666 MsG0280007500.01.T01:intron 25.0%
! GGTTCAAAGTACAAAAAATA+TGG + Chr2:16343255-16343274 MsG0280007500.01.T01:intron 25.0%
! GTTAGTGTTATCATCTTTAA+AGG - Chr2:16344806-16344825 None:intergenic 25.0%
! GTTGCAAAAAATAAATTCCA+GGG + Chr2:16343232-16343251 MsG0280007500.01.T01:intron 25.0%
! TACCTTAATTTCTTCATCTT+TGG - Chr2:16346202-16346221 None:intergenic 25.0%
! TGAAAAAGAAAATGAGATAC+AGG + Chr2:16346011-16346030 MsG0280007500.01.T01:CDS 25.0%
! TTAGTATAATTTCAGTGGTA+AGG + Chr2:16343768-16343787 MsG0280007500.01.T01:intron 25.0%
! TTGCAAAAAATAAATTCCAG+GGG + Chr2:16343233-16343252 MsG0280007500.01.T01:intron 25.0%
! TTGTGTTAGTATAATTTCAG+TGG + Chr2:16343763-16343782 MsG0280007500.01.T01:intron 25.0%
!! ATATTCGATTTATCAGCTTT+TGG - Chr2:16345763-16345782 None:intergenic 25.0%
!! ATCACAGTTTTCTTTGAATA+TGG + Chr2:16344108-16344127 MsG0280007500.01.T01:intron 25.0%
!! ATGTGTTGAAAAAAGGTATA+TGG + Chr2:16345959-16345978 MsG0280007500.01.T01:CDS 25.0%
!! GTAATTTTATAGCATTGTTC+AGG - Chr2:16346659-16346678 None:intergenic 25.0%
!! TAATACAACTTTTCATGAAC+AGG + Chr2:16345085-16345104 MsG0280007500.01.T01:intron 25.0%
!! TCAAGAAACAAATTTTCTGA+TGG + Chr2:16345985-16346004 MsG0280007500.01.T01:CDS 25.0%
!! TGTGTTGAAAAAAGGTATAT+GGG + Chr2:16345960-16345979 MsG0280007500.01.T01:CDS 25.0%
!! TTAAATACCTTTTGTTACCT+TGG - Chr2:16343862-16343881 None:intergenic 25.0%
!! TTTTAACACATTAACAATGG+TGG - Chr2:16343911-16343930 None:intergenic 25.0%
!! TTTTAGCATAAAATTTCAGG+AGG + Chr2:16345359-16345378 MsG0280007500.01.T01:intron 25.0%
!!! ACCTTTTTTCAACACATTTT+CGG - Chr2:16345956-16345975 None:intergenic 25.0%
!!! CTTTAGTTCTATGTAACAAA+AGG - Chr2:16346410-16346429 None:intergenic 25.0%
!!! GCACTTTTTTTTTTATCGTA+AGG + Chr2:16343809-16343828 MsG0280007500.01.T01:intron 25.0%
!!! TGGTTTTAGCATAAAATTTC+AGG + Chr2:16345356-16345375 MsG0280007500.01.T01:intron 25.0%
AAAAATCAATAGATCAAGGG+AGG + Chr2:16344052-16344071 MsG0280007500.01.T01:intron 30.0%
AAAAGCAGAATCACAATTGA+AGG + Chr2:16345575-16345594 MsG0280007500.01.T01:CDS 30.0%
AACACCAATGGAAAATTAGA+TGG + Chr2:16343683-16343702 MsG0280007500.01.T01:intron 30.0%
AACATAAGAAGATATATGCC+TGG - Chr2:16345013-16345032 None:intergenic 30.0%
AACGCATCATTAATGGAAAT+TGG - Chr2:16344751-16344770 None:intergenic 30.0%
AACTGAAGCTAAGAAAGATA+AGG + Chr2:16344251-16344270 MsG0280007500.01.T01:CDS 30.0%
AAGATTACACACAGATCAAA+AGG + Chr2:16344643-16344662 MsG0280007500.01.T01:CDS 30.0%
AAGATTTCAACAAATCGCTA+TGG - Chr2:16346366-16346385 None:intergenic 30.0%
ACCGAAAATGTGTTGAAAAA+AGG + Chr2:16345952-16345971 MsG0280007500.01.T01:CDS 30.0%
AGAAGAAAGAAAGATTATGC+AGG + Chr2:16345503-16345522 MsG0280007500.01.T01:CDS 30.0%
AGAGGATCATCTTAAAAACA+AGG + Chr2:16344478-16344497 MsG0280007500.01.T01:CDS 30.0%
AGATGAAGAAATTAAGGTAC+TGG + Chr2:16346203-16346222 MsG0280007500.01.T01:intron 30.0%
ATAACCAAAATGAAGACCAA+AGG + Chr2:16343363-16343382 MsG0280007500.01.T01:intron 30.0%
ATAGAGACTATACACGAAAA+TGG - Chr2:16345037-16345056 None:intergenic 30.0%
ATGGAAAGATGAAAGTGATA+AGG + Chr2:16345458-16345477 MsG0280007500.01.T01:CDS 30.0%
CAATGCTATAAAATTACTGC+AGG + Chr2:16346662-16346681 MsG0280007500.01.T01:CDS 30.0%
CACCAAAGATGAAGAAATTA+AGG + Chr2:16346197-16346216 MsG0280007500.01.T01:CDS 30.0%
CATCAATAAAATCCGATGAT+AGG + Chr2:16346544-16346563 MsG0280007500.01.T01:CDS 30.0%
CATTGTAAACGCATCATTAA+TGG - Chr2:16344758-16344777 None:intergenic 30.0%
CTAACACAAGACCAAAAATA+AGG - Chr2:16343752-16343771 None:intergenic 30.0%
CTTTATGAAGAGAAAATTGG+AGG + Chr2:16346708-16346727 MsG0280007500.01.T01:CDS 30.0%
CTTTGCAAAACAAAACTCAT+AGG + Chr2:16343595-16343614 MsG0280007500.01.T01:CDS 30.0%
GAAGTTCAAACTTCTCTAAA+AGG + Chr2:16346577-16346596 MsG0280007500.01.T01:CDS 30.0%
GAGAAATGAAGTAAGTAAGA+TGG + Chr2:16344436-16344455 MsG0280007500.01.T01:CDS 30.0%
GATTAAGATGTATAGAACTG+AGG + Chr2:16344715-16344734 MsG0280007500.01.T01:CDS 30.0%
GCAAAAAAGAAGTCAGATAA+CGG - Chr2:16346324-16346343 None:intergenic 30.0%
GGTTGCAAAAAATAAATTCC+AGG + Chr2:16343231-16343250 MsG0280007500.01.T01:intron 30.0%
GGTTTATCTATACAGTAACA+TGG + Chr2:16345336-16345355 MsG0280007500.01.T01:intron 30.0%
TCATGACTAAAAAGTGATAG+TGG + Chr2:16343970-16343989 MsG0280007500.01.T01:intron 30.0%
TGCAAAAAATAAATTCCAGG+GGG + Chr2:16343234-16343253 MsG0280007500.01.T01:intron 30.0%
TGTATAGTCTCTATATCGAA+CGG + Chr2:16345043-16345062 MsG0280007500.01.T01:intron 30.0%
TTAAGTTTCGTAACGAACAA+TGG + Chr2:16345784-16345803 MsG0280007500.01.T01:CDS 30.0%
TTCCAAAAGCCAAACATAAA+AGG + Chr2:16345865-16345884 MsG0280007500.01.T01:CDS 30.0%
TTGTCAATCGTAGAAAATAG+TGG + Chr2:16346254-16346273 MsG0280007500.01.T01:intron 30.0%
! AGAAACAAATTTTCTGATGG+TGG + Chr2:16345988-16346007 MsG0280007500.01.T01:CDS 30.0%
! CATAGGAATTTTGTATCTAC+TGG + Chr2:16345315-16345334 MsG0280007500.01.T01:intron 30.0%
! GGTAGAACCATATTTCATTT+TGG - Chr2:16345935-16345954 None:intergenic 30.0%
! TCTATGTTGCCTTTTATGTT+TGG - Chr2:16345877-16345896 None:intergenic 30.0%
! TGTTATGACTACTTGCATTT+TGG + Chr2:16345281-16345300 MsG0280007500.01.T01:intron 30.0%
! TTTAAGTGAACTCTTTTCCA+AGG + Chr2:16343842-16343861 MsG0280007500.01.T01:intron 30.0%
! TTTTGGACTAACTTTTGCAT+AGG + Chr2:16345298-16345317 MsG0280007500.01.T01:intron 30.0%
! TTTTTGTACTTAGCCTCAAT+AGG + Chr2:16343414-16343433 MsG0280007500.01.T01:intron 30.0%
!! AAGATAAGAAAGCTTTGGAA+CGG + Chr2:16344859-16344878 MsG0280007500.01.T01:CDS 30.0%
!! GTTTTAATCTGTTGCAGAAA+AGG + Chr2:16344182-16344201 MsG0280007500.01.T01:intron 30.0%
!! TATACGGTATTATTTTTCGC+AGG + Chr2:16345657-16345676 MsG0280007500.01.T01:intron 30.0%
!! TTTTCCTTTGGTCTTCATTT+TGG - Chr2:16343370-16343389 None:intergenic 30.0%
!!! CTATGAGTTTTGTTTTGCAA+AGG - Chr2:16343597-16343616 None:intergenic 30.0%
AAAACTTTGACCTGAAGAGA+CGG + Chr2:16344976-16344995 MsG0280007500.01.T01:intron 35.0%
AAAAGGCAACATAGACAGTA+TGG + Chr2:16345882-16345901 MsG0280007500.01.T01:CDS 35.0%
AAATCTTAGAGAAAAGCCAC+CGG + Chr2:16345117-16345136 MsG0280007500.01.T01:CDS 35.0%
AGAAGGCTTAAAGCAATTCT+CGG + Chr2:16345711-16345730 MsG0280007500.01.T01:CDS 35.0%
ATAGGTGTTCTACTTTGTGA+TGG - Chr2:16343446-16343465 None:intergenic 35.0%
ATCACAAAGTAGAACACCTA+TGG + Chr2:16343445-16343464 MsG0280007500.01.T01:intron 35.0%
ATCTTAATCTCGTCTTCAAG+AGG - Chr2:16344704-16344723 None:intergenic 35.0%
CAACAAATGAAGTATGTCAC+TGG - Chr2:16343487-16343506 None:intergenic 35.0%
CAATAAAATCCGATGATAGG+AGG + Chr2:16346547-16346566 MsG0280007500.01.T01:CDS 35.0%
CTGTCAGTTTCTTGATTTCT+AGG - Chr2:16344571-16344590 None:intergenic 35.0%
GAAAATGGTTGTCAATGAAG+AGG + Chr2:16343530-16343549 MsG0280007500.01.T01:exon 35.0%
GACAACTCTGATATGATATG+AGG - Chr2:16346239-16346258 None:intergenic 35.0%
GTAATCACGAATGAGATTAG+CGG - Chr2:16345219-16345238 None:intergenic 35.0%
GTACAAAAAATATGGGGTAC+TGG + Chr2:16343263-16343282 MsG0280007500.01.T01:intron 35.0%
TAAAATTTCAGGAGGAGAAG+AGG + Chr2:16345367-16345386 MsG0280007500.01.T01:intron 35.0%
TCAGGAAGATAAGAAAGCTT+TGG + Chr2:16344854-16344873 MsG0280007500.01.T01:CDS 35.0%
TCATGCATGTAAAACACCAA+TGG + Chr2:16343671-16343690 MsG0280007500.01.T01:intron 35.0%
TGAGTGTTAGAAAGATTGTC+TGG + Chr2:16343204-16343223 MsG0280007500.01.T01:intron 35.0%
TGGAGATACTGAAAATGCAT+TGG - Chr2:16345743-16345762 None:intergenic 35.0%
TTAGAAAGATTGTCTGGTCA+AGG + Chr2:16343210-16343229 MsG0280007500.01.T01:intron 35.0%
! CTCTTTTCCAAGGTAACAAA+AGG + Chr2:16343852-16343871 MsG0280007500.01.T01:intron 35.0%
! GACAACCATTTTCTTCTTTC+TGG - Chr2:16343523-16343542 None:intergenic 35.0%
! GAGTCCATCTAATTTTCCAT+TGG - Chr2:16343690-16343709 None:intergenic 35.0%
! TACAACTTTTCATGAACAGG+AGG + Chr2:16345088-16345107 MsG0280007500.01.T01:intron 35.0%
! TTGGAACAAAGTGTTTCCAA+CGG - Chr2:16345851-16345870 None:intergenic 35.0%
! TTTAGAGTCTTCATCAGCAT+TGG - Chr2:16346058-16346077 None:intergenic 35.0%
!! GAAAGTTGATGCATTGACAA+AGG + Chr2:16346461-16346480 MsG0280007500.01.T01:CDS 35.0%
!! TGATTTAGAAAGCACTGATG+TGG + Chr2:16346092-16346111 MsG0280007500.01.T01:CDS 35.0%
!! TGGTGGATTTATTAAGTCCA+GGG + Chr2:16343283-16343302 MsG0280007500.01.T01:intron 35.0%
!!! TGCCTTTTATGTTTGGCTTT+TGG - Chr2:16345870-16345889 None:intergenic 35.0%
!!! TTTTGCTGTTCTCTCTGATA+TGG - Chr2:16345559-16345578 None:intergenic 35.0%
AACATGCCATGGTGAAATCA+AGG + Chr2:16344218-16344237 MsG0280007500.01.T01:CDS 40.0%
AATGAACTTGCAACATGCCA+TGG + Chr2:16344207-16344226 MsG0280007500.01.T01:CDS 40.0%
AATGAGATACAGGTGAACAC+AGG + Chr2:16346021-16346040 MsG0280007500.01.T01:CDS 40.0%
ACTAGAGAGGGAATTAGCAA+GGG + Chr2:16345392-16345411 MsG0280007500.01.T01:CDS 40.0%
AGACGAAAAGAAAGATGCGT+TGG + Chr2:16344589-16344608 MsG0280007500.01.T01:CDS 40.0%
AGGCTAAAAACGCTTGAAGA+AGG + Chr2:16345694-16345713 MsG0280007500.01.T01:CDS 40.0%
AGTTTCGTAACGAACAATGG+TGG + Chr2:16345787-16345806 MsG0280007500.01.T01:CDS 40.0%
ATCTCCAACACAACAACATC+TGG - Chr2:16343570-16343589 None:intergenic 40.0%
ATGGTGAAATCAAGGCCATA+AGG + Chr2:16344226-16344245 MsG0280007500.01.T01:CDS 40.0%
CAAACCAGATGTTGTTGTGT+TGG + Chr2:16343563-16343582 MsG0280007500.01.T01:CDS 40.0%
CAAAGGCTATGGAAGTAGAA+TGG + Chr2:16346478-16346497 MsG0280007500.01.T01:CDS 40.0%
CAACAGAATTGCAACTGTTG+CGG + Chr2:16345428-16345447 MsG0280007500.01.T01:CDS 40.0%
CAGATTTCTGAGTTGCAAGT+AGG + Chr2:16345245-16345264 MsG0280007500.01.T01:intron 40.0%
CCAAACGATTCATGAACTTG+AGG + Chr2:16345169-16345188 MsG0280007500.01.T01:CDS 40.0%
CCTCAAGTTCATGAATCGTT+TGG - Chr2:16345172-16345191 None:intergenic 40.0%
GAAAGATAAGGCCATAGAAG+AGG + Chr2:16344263-16344282 MsG0280007500.01.T01:CDS 40.0%
GAGGAGCTCATCTTATCAAA+TGG + Chr2:16345188-16345207 MsG0280007500.01.T01:CDS 40.0%
GTGGATTTATTAAGTCCAGG+GGG + Chr2:16343285-16343304 MsG0280007500.01.T01:intron 40.0%
TCTACAGATTACAGCACTTC+AGG + Chr2:16344836-16344855 MsG0280007500.01.T01:intron 40.0%
TCTTCTAACCATTGCCTAAC+AGG - Chr2:16345489-16345508 None:intergenic 40.0%
TGATTCTGCAGCATATTGTG+CGG - Chr2:16344615-16344634 None:intergenic 40.0%
TGCGAGACTAAGACTTAAAG+AGG + Chr2:16344460-16344479 MsG0280007500.01.T01:CDS 40.0%
TGGTGAAATCAAGGCCATAA+GGG + Chr2:16344227-16344246 MsG0280007500.01.T01:CDS 40.0%
! ACAACATCTGGTTTGACACT+AGG - Chr2:16343558-16343577 None:intergenic 40.0%
! CAACTTCCACAATCTTTTGC+CGG - Chr2:16345139-16345158 None:intergenic 40.0%
! GGTGGATTTATTAAGTCCAG+GGG + Chr2:16343284-16343303 MsG0280007500.01.T01:intron 40.0%
! TAGATGGACTCATTGTGTCA+AGG + Chr2:16343699-16343718 MsG0280007500.01.T01:intron 40.0%
! TGATGCATTGACAAAGGCTA+TGG + Chr2:16346467-16346486 MsG0280007500.01.T01:CDS 40.0%
! TTATGGCCTTGATTTCACCA+TGG - Chr2:16344227-16344246 None:intergenic 40.0%
! TTTTTGTACTTTGAACCCCC+TGG - Chr2:16343252-16343271 None:intergenic 40.0%
!! ACAATGGTGGATTGAGAAAC+AGG + Chr2:16345800-16345819 MsG0280007500.01.T01:CDS 40.0%
!! CAAAAAATATGGGGTACTGG+TGG + Chr2:16343266-16343285 MsG0280007500.01.T01:intron 40.0%
!! CTGGTGGATTTATTAAGTCC+AGG + Chr2:16343282-16343301 MsG0280007500.01.T01:intron 40.0%
!! GAAGTGCTGTAATCTGTAGA+TGG - Chr2:16344836-16344855 None:intergenic 40.0%
!! TCTGCTTTTCCTCCTATCAT+CGG - Chr2:16346559-16346578 None:intergenic 40.0%
!! TGGTTATGCTACCCGTTTTT+CGG - Chr2:16345915-16345934 None:intergenic 40.0%
AGGTCTACATCACAACCAAG+AGG + Chr2:16345820-16345839 MsG0280007500.01.T01:CDS 45.0%
ATCACGAATGAGATTAGCGG+TGG - Chr2:16345216-16345235 None:intergenic 45.0%
ATGTAGTGTACTGTTGAGCC+AGG - Chr2:16343345-16343364 None:intergenic 45.0%
CAACTGTTGCGGCTAATGAA+TGG + Chr2:16345439-16345458 MsG0280007500.01.T01:CDS 45.0%
CACTAGAGAGGGAATTAGCA+AGG + Chr2:16345391-16345410 MsG0280007500.01.T01:CDS 45.0%
CGCAACAGTTGCAATTCTGT+TGG - Chr2:16345430-16345449 None:intergenic 45.0%
CTTAGCTTCAGTTGCCCTTA+TGG - Chr2:16344244-16344263 None:intergenic 45.0%
GCAACATAGACAGTATGGCT+GGG + Chr2:16345887-16345906 MsG0280007500.01.T01:CDS 45.0%
GCTCACAAAGTCGAAAGAGT+TGG + Chr2:16344881-16344900 MsG0280007500.01.T01:CDS 45.0%
GGCGTGATGACACTTTCAAA+TGG - Chr2:16344683-16344702 None:intergenic 45.0%
GTAGAGAGAAGGATAGAGAG+AGG + Chr2:16343154-16343173 MsG0280007500.01.T01:intron 45.0%
GTGATAAGGTCATGCCTGTT+AGG + Chr2:16345472-16345491 MsG0280007500.01.T01:CDS 45.0%
GTGTGTAGTCAGTAGAGAGA+AGG + Chr2:16343143-16343162 MsG0280007500.01.T01:intron 45.0%
TATGGCTGGGAACCGAAAAA+CGG + Chr2:16345900-16345919 MsG0280007500.01.T01:CDS 45.0%
TGTAGTGTACTGTTGAGCCA+GGG - Chr2:16343344-16343363 None:intergenic 45.0%
TTATGCAGGCAGAGATGCAA+AGG + Chr2:16345517-16345536 MsG0280007500.01.T01:CDS 45.0%
TTTCCAACGGTAGATCCTCT+TGG - Chr2:16345838-16345857 None:intergenic 45.0%
!! GTGATGGAAAGTGCCTATTG+AGG - Chr2:16343430-16343449 None:intergenic 45.0%
!!! TAGTAATAAATAATTTTATA+CGG + Chr2:16345641-16345660 MsG0280007500.01.T01:intron 5.0%
AAGCCACCGGCAAAAGATTG+TGG + Chr2:16345130-16345149 MsG0280007500.01.T01:CDS 50.0%
AGATCTCGATCCGTCTCTTC+AGG - Chr2:16344989-16345008 None:intergenic 50.0%
AGGAGAAGAGGACACTAGAG+AGG + Chr2:16345379-16345398 MsG0280007500.01.T01:CDS 50.0%
AGGTCATGCCTGTTAGGCAA+TGG + Chr2:16345478-16345497 MsG0280007500.01.T01:CDS 50.0%
ATGGCTGGGAACCGAAAAAC+GGG + Chr2:16345901-16345920 MsG0280007500.01.T01:CDS 50.0%
CAACCAAGAGGATCTACCGT+TGG + Chr2:16345832-16345851 MsG0280007500.01.T01:CDS 50.0%
CGCAGGATAAACTGAAGCTG+AGG + Chr2:16345674-16345693 MsG0280007500.01.T01:intron 50.0%
CGGAGCTACATAGTAGTGAG+GGG - Chr2:16343321-16343340 None:intergenic 50.0%
GCGGAGCTACATAGTAGTGA+GGG - Chr2:16343322-16343341 None:intergenic 50.0%
GGAGAAGAGGACACTAGAGA+GGG + Chr2:16345380-16345399 MsG0280007500.01.T01:CDS 50.0%
GGAGCTACATAGTAGTGAGG+GGG - Chr2:16343320-16343339 None:intergenic 50.0%
GGCAACATAGACAGTATGGC+TGG + Chr2:16345886-16345905 MsG0280007500.01.T01:CDS 50.0%
TGGCATTGCTTGAAGCAGAG+AGG + Chr2:16344901-16344920 MsG0280007500.01.T01:CDS 50.0%
! CTTCCACAATCTTTTGCCGG+TGG - Chr2:16345136-16345155 None:intergenic 50.0%
!! GAAAGCACTGATGTGGTCTC+TGG + Chr2:16346099-16346118 MsG0280007500.01.T01:CDS 50.0%
ACTACTATGTAGCTCCGCCC+TGG + Chr2:16343324-16343343 MsG0280007500.01.T01:intron 55.0%
AGTGTACTGTTGAGCCAGGG+CGG - Chr2:16343341-16343360 None:intergenic 55.0%
GGCGGAGCTACATAGTAGTG+AGG - Chr2:16343323-16343342 None:intergenic 55.0%
GAGACGGATCGAGATCTGCC+AGG + Chr2:16344992-16345011 MsG0280007500.01.T01:intron 60.0%
AGGGGGTTCACTGAACCCCC+TGG - Chr2:16343303-16343322 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 16343124 16346830 16343124 ID=MsG0280007500.01;Name=MsG0280007500.01
Chr2 mRNA 16343124 16346830 16343124 ID=MsG0280007500.01.T01;Parent=MsG0280007500.01;Name=MsG0280007500.01.T01;_AED=0.28;_eAED=0.35;_QI=36|0.6|0.45|0.90|0.6|0.54|11|0|620
Chr2 exon 16343124 16343149 16343124 ID=MsG0280007500.01.T01:exon:4286;Parent=MsG0280007500.01.T01
Chr2 exon 16343524 16343616 16343524 ID=MsG0280007500.01.T01:exon:4287;Parent=MsG0280007500.01.T01
Chr2 exon 16343783 16343825 16343783 ID=MsG0280007500.01.T01:exon:4288;Parent=MsG0280007500.01.T01
Chr2 exon 16344204 16344284 16344204 ID=MsG0280007500.01.T01:exon:4289;Parent=MsG0280007500.01.T01
Chr2 exon 16344434 16344499 16344434 ID=MsG0280007500.01.T01:exon:4290;Parent=MsG0280007500.01.T01
Chr2 exon 16344566 16344736 16344566 ID=MsG0280007500.01.T01:exon:4291;Parent=MsG0280007500.01.T01
Chr2 exon 16344843 16344965 16344843 ID=MsG0280007500.01.T01:exon:4292;Parent=MsG0280007500.01.T01
Chr2 exon 16345107 16345262 16345107 ID=MsG0280007500.01.T01:exon:4293;Parent=MsG0280007500.01.T01
Chr2 exon 16345378 16345596 16345378 ID=MsG0280007500.01.T01:exon:4294;Parent=MsG0280007500.01.T01
Chr2 exon 16345679 16346218 16345679 ID=MsG0280007500.01.T01:exon:4295;Parent=MsG0280007500.01.T01
Chr2 exon 16346450 16346830 16346450 ID=MsG0280007500.01.T01:exon:4296;Parent=MsG0280007500.01.T01
Chr2 five_prime_UTR 16343124 16343149 16343124 ID=MsG0280007500.01.T01:five_prime_utr;Parent=MsG0280007500.01.T01
Chr2 five_prime_UTR 16343524 16343533 16343524 ID=MsG0280007500.01.T01:five_prime_utr;Parent=MsG0280007500.01.T01
Chr2 CDS 16343534 16343616 16343534 ID=MsG0280007500.01.T01:cds;Parent=MsG0280007500.01.T01
Chr2 CDS 16343783 16343825 16343783 ID=MsG0280007500.01.T01:cds;Parent=MsG0280007500.01.T01
Chr2 CDS 16344204 16344284 16344204 ID=MsG0280007500.01.T01:cds;Parent=MsG0280007500.01.T01
Chr2 CDS 16344434 16344499 16344434 ID=MsG0280007500.01.T01:cds;Parent=MsG0280007500.01.T01
Chr2 CDS 16344566 16344736 16344566 ID=MsG0280007500.01.T01:cds;Parent=MsG0280007500.01.T01
Chr2 CDS 16344843 16344965 16344843 ID=MsG0280007500.01.T01:cds;Parent=MsG0280007500.01.T01
Chr2 CDS 16345107 16345262 16345107 ID=MsG0280007500.01.T01:cds;Parent=MsG0280007500.01.T01
Chr2 CDS 16345378 16345596 16345378 ID=MsG0280007500.01.T01:cds;Parent=MsG0280007500.01.T01
Chr2 CDS 16345679 16346218 16345679 ID=MsG0280007500.01.T01:cds;Parent=MsG0280007500.01.T01
Chr2 CDS 16346450 16346830 16346450 ID=MsG0280007500.01.T01:cds;Parent=MsG0280007500.01.T01
Gene Sequence

>MsG0280007500.01.T01

ATGGTTGTCAATGAAGAGGTTCCTAGTGTCAAACCAGATGTTGTTGTGTTGGAGATTGATCCTTTGCAAAACAAAACTCATAGTGGTAAGGAAACTGAAAAAAATGCATGCACTTTTTTTTTTATCGTTAATGAACTTGCAACATGCCATGGTGAAATCAAGGCCATAAGGGCAACTGAAGCTAAGAAAGATAAGGCCATAGAAGAGCTGAGAAATGAAGTAAGTAAGATGGATGCGAGACTAAGACTTAAAGAGGATCATCTTAAAAACAAGAACCTAGAAATCAAGAAACTGACAGACGAAAAGAAAGATGCGTTGGCCGCACAATATGCTGCAGAATCAGCACTTAGAAGATTACACACAGATCAAAAGGAAGAAGATTTTTATCCATTTGAAAGTGTCATCACGCCTCTTGAAGACGAGATTAAGATGTATAGAACTGAGATTACAGCACTTCAGGAAGATAAGAAAGCTTTGGAACGGCTCACAAAGTCGAAAGAGTTGGCATTGCTTGAAGCAGAGAGGATTTTACGAAGTGCACTAGAGAGAGCTTTGATAGTCGAAGAAGAGGAGAACAAAATCTTAGAGAAAAGCCACCGGCAAAAGATTGTGGAAGTTGAGAAACTTAGCCAAACGATTCATGAACTTGAGGAGCTCATCTTATCAAATGGAGCCACCGCTAATCTCATTCGTGATTACAAGCGACAGATTTCTGAGTTGCAAGAGGAGAAGAGGACACTAGAGAGGGAATTAGCAAGGGTAAAAGTTTCTGCCAACAGAATTGCAACTGTTGCGGCTAATGAATGGAAAGATGAAAGTGATAAGGTCATGCCTGTTAGGCAATGGTTAGAAGAAAGAAAGATTATGCAGGCAGAGATGCAAAGGTTGAAAGAAAAGCTTGCCATATCAGAGAGAACAGCAAAAGCAGAATCACAATTGAAGGATAAACTGAAGCTGAGGCTAAAAACGCTTGAAGAAGGCTTAAAGCAATTCTCGGTCAATTCCAATGCATTTTCAGTATCTCCAAAAGCTGATAAATCGAATATCTTAAGTTTCGTAACGAACAATGGTGGATTGAGAAACAGGTCTACATCACAACCAAGAGGATCTACCGTTGGAAACACTTTGTTCCAAAAGCCAAACATAAAAGGCAACATAGACAGTATGGCTGGGAACCGAAAAACGGGTAGCATAACCAAAATGAAATATGGTTCTACCGAAAATGTGTTGAAAAAAGGTATATGGGCGTCAAGAAACAAATTTTCTGATGGTGGTGAAAAAGAAAATGAGATACAGGTGAACACAGGCAGTGTTAAAACCAATGCTGATGAAGACTCTAAAAGTAATAGCAGCAATGATTTAGAAAGCACTGATGTGGTCTCTGGATTTTTATATGATAAGCTTCAAAAAGAAGTCATCAATTTGAGGAAGACTTGTGAAACAAAAGACAATACTCTGCACACCAAAGATGAAGAAATTAAGATGCTCACAAAGAAAGTTGATGCATTGACAAAGGCTATGGAAGTAGAATGGAAAAAAATGAAAAGAGAAATAGCTGCAAGAGAGAAAGAAGTTGCATCAATAAAATCCGATGATAGGAGGAAAAGCAGAAGTTCAAACTTCTCTAAAAGGTTATCTTCTGTCACTTTTTCTTTTGAAAGATTGATACCTTTTACTTCTATAGTTAACCTGAACAATGCTATAAAATTACTGCAGGGTGATGAAAGAACATTGAAGATTCTTTATGAAGAGAAAATTGGAGGCAATTTATGCATTAATGCATCTTATGATTTTTCTTTGTATACACAACATAATTCAGTGTGCAGTTTGTTTAATAAAGTTCCACTAACTTACAGAGTCTGA
Protein sequence

>MsG0280007500.01.T01

MVVNEEVPSVKPDVVVLEIDPLQNKTHSGKETEKNACTFFFIVNELATCHGEIKAIRATEAKKDKAIEELRNEVSKMDARLRLKEDHLKNKNLEIKKLTDEKKDALAAQYAAESALRRLHTDQKEEDFYPFESVITPLEDEIKMYRTEITALQEDKKALERLTKSKELALLEAERILRSALERALIVEEEENKILEKSHRQKIVEVEKLSQTIHELEELILSNGATANLIRDYKRQISELQEEKRTLERELARVKVSANRIATVAANEWKDESDKVMPVRQWLEERKIMQAEMQRLKEKLAISERTAKAESQLKDKLKLRLKTLEEGLKQFSVNSNAFSVSPKADKSNILSFVTNNGGLRNRSTSQPRGSTVGNTLFQKPNIKGNIDSMAGNRKTGSITKMKYGSTENVLKKGIWASRNKFSDGGEKENEIQVNTGSVKTNADEDSKSNSSNDLESTDVVSGFLYDKLQKEVINLRKTCETKDNTLHTKDEEIKMLTKKVDALTKAMEVEWKKMKREIAAREKEVASIKSDDRRKSRSSNFSKRLSSVTFSFERLIPFTSIVNLNNAIKLLQGDERTLKILYEEKIGGNLCINASYDFSLYTQHNSVCSLFNKVPLTYRV*