AlfalfaGEDB | MsG0880046186.01

M. sativa cultivar ZhongmuNo.1 / MsG0880046186.01

KEGG: K09422
GO: GO:0003677
PFAM: PF00249

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046186.01.T01	XP_013457650.1	98.162	272	5	0	1	272	1	272	0	553

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046186.01.T01	Q50EX6	56.856	299	96	6	1	272	1	293	6.77E-113	330

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046186.01.T01	A0A072UQP4	98.162	272	5	0	1	272	1	272	0.0	553

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TFs/TRs:

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Gene ID	Type	Classification
MsG0880046186.01.T01	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180001117.01	MsG0880046186.01	0.810781	9.716873e-51	5.911287e-48
MsG0180005188.01	MsG0880046186.01	0.820800	5.662984e-53	4.526101e-50
MsG0280007500.01	MsG0880046186.01	0.805361	1.385493e-49	7.315612e-47
MsG0280008909.01	MsG0880046186.01	0.805635	1.213893e-49	6.454784e-47
MsG0380013548.01	MsG0880046186.01	0.818591	1.809950e-52	1.360489e-49
MsG0380016348.01	MsG0880046186.01	0.800709	1.269273e-48	5.950535e-46
MsG0480020331.01	MsG0880046186.01	0.807583	4.708388e-50	2.632243e-47
MsG0480021923.01	MsG0880046186.01	0.821181	4.629224e-53	3.739478e-50
MsG0580024405.01	MsG0880046186.01	0.806702	7.235335e-50	3.953679e-47
MsG0580024793.01	MsG0880046186.01	0.804520	2.076777e-49	1.073009e-46

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PPI

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Gene1	Gene2	Type
MsG0380016901.01	MsG0880046186.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046186.01.T01	MTR_4g102380	98.162	272	5	0	1	272	1	272	0.0	553
MsG0880046186.01.T01	MTR_5g078140	57.658	222	78	3	1	207	1	221	1.35e-85	258
MsG0880046186.01.T01	MTR_3g065440	62.617	214	71	3	1	206	1	213	4.93e-84	254
MsG0880046186.01.T01	MTR_6g012690	64.000	150	53	1	1	149	1	150	1.07e-66	211
MsG0880046186.01.T01	MTR_7g011170	67.939	131	42	0	1	131	1	131	1.15e-66	210
MsG0880046186.01.T01	MTR_4g082290	65.541	148	49	1	1	148	1	146	7.28e-66	211
MsG0880046186.01.T01	MTR_3g074520	69.531	128	39	0	1	128	1	128	1.19e-65	208
MsG0880046186.01.T01	MTR_8g031360	72.519	131	34	2	1	129	1	131	3.26e-65	206
MsG0880046186.01.T01	MTR_4g478180	68.750	128	40	0	1	128	2	129	8.22e-65	206
MsG0880046186.01.T01	MTR_4g073420	61.644	146	56	0	1	146	1	146	3.70e-64	202

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880046186.01.T01	AT4G12350	56.383	282	105	6	1	264	1	282	8.61e-104	303
MsG0880046186.01.T01	AT4G22680	61.278	266	88	5	1	251	1	266	2.69e-103	301
MsG0880046186.01.T01	AT1G66230	59.716	211	65	4	1	208	1	194	4.07e-83	251
MsG0880046186.01.T01	AT5G16600	82.963	135	23	0	1	135	1	135	1.08e-81	249
MsG0880046186.01.T01	AT5G62320	64.198	162	48	2	2	156	3	161	1.23e-71	220
MsG0880046186.01.T01	AT5G14340	61.392	158	61	0	1	158	13	170	7.52e-68	212
MsG0880046186.01.T01	AT3G61250	63.087	149	55	0	1	149	1	149	6.54e-67	210
MsG0880046186.01.T01	AT4G28110	69.531	128	39	0	1	128	1	128	7.79e-67	209
MsG0880046186.01.T01	AT5G15310	68.702	131	41	0	1	131	1	131	9.62e-67	211
MsG0880046186.01.T01	AT3G02940	71.094	128	37	0	1	128	1	128	1.04e-66	210

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Find 71 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGATTGCTTCACGGTTACC+CGG	0.461826	8:-70256139	MsG0880046186.01.T01:CDS
AATCTCATTGTCTGTTCTCC+CGG	0.377399	8:+70256120	None:intergenic
ACACCAAGTTTGTCACAACA+AGG	0.629808	8:+70256901	None:intergenic
ACCATTGGTGGAAGCTCTTT+GGG	0.294356	8:-70255801	MsG0880046186.01.T01:CDS
ACCTGTTGCCAAGACGGGCA+TGG	0.540749	8:+70256671	None:intergenic
ACGCCAACAACATTGTCCAT+TGG	0.609352	8:+70256822	None:intergenic
ACTAAATAAAACTCAACACA+AGG	0.652484	8:+70255526	None:intergenic
ACTGCAAACTATAGGGATGA+AGG	0.532537	8:-70255627	MsG0880046186.01.T01:CDS
AGATTGCTTCACGGTTACCC+GGG	0.595542	8:-70256138	MsG0880046186.01.T01:CDS
AGCTCTTACCGCAGCGCCGA+AGG	0.506544	8:+70256779	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGTGTTTCAAAGAATGTTCT+TGG	-	Chr8:70256697-70256716	MsG0880046186.01.T01:CDS	30.0%
ATATAGTTGATTGTTGTCTC+AGG	-	Chr8:70256031-70256050	MsG0880046186.01.T01:CDS	30.0%
ATTCAAAGGTTCATGAGTAA+TGG	+	Chr8:70256160-70256179	None:intergenic	30.0%
CAAGAAAAAGCTCATTAAGA+TGG	-	Chr8:70256125-70256144	MsG0880046186.01.T01:CDS	30.0%
GATGATGCTTGTTTATTCAA+AGG	+	Chr8:70256174-70256193	None:intergenic	30.0%
TGATAGCAAGCCAAAAATAA+TGG	-	Chr8:70256777-70256796	MsG0880046186.01.T01:CDS	30.0%
TTCAAAGGTTCATGAGTAAT+GGG	+	Chr8:70256159-70256178	None:intergenic	30.0%
ATGAAGTGAAGGAAACTGAT+GGG	-	Chr8:70256250-70256269	MsG0880046186.01.T01:intron	35.0%
CAAAGAATGTTCTTGGAAAG+TGG	-	Chr8:70256704-70256723	MsG0880046186.01.T01:CDS	35.0%
CAAGTACACTGCAAACTATA+GGG	-	Chr8:70256565-70256584	MsG0880046186.01.T01:intron	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	70255245	70256976	70255245	ID=MsG0880046186.01;Name=MsG0880046186.01
Chr8	mRNA	70255245	70256976	70255245	ID=MsG0880046186.01.T01;Parent=MsG0880046186.01;Name=MsG0880046186.01.T01;_AED=0.43;_eAED=0.43;_QI=41\|1\|1\|1\|1\|1\|2\|368\|272
Chr8	exon	70255245	70256168	70255245	ID=MsG0880046186.01.T01:exon:27472;Parent=MsG0880046186.01.T01
Chr8	exon	70256673	70256976	70256673	ID=MsG0880046186.01.T01:exon:27471;Parent=MsG0880046186.01.T01
Chr8	five_prime_UTR	70256936	70256976	70256936	ID=MsG0880046186.01.T01:five_prime_utr;Parent=MsG0880046186.01.T01
Chr8	CDS	70256673	70256935	70256673	ID=MsG0880046186.01.T01:cds;Parent=MsG0880046186.01.T01
Chr8	CDS	70255613	70256168	70255613	ID=MsG0880046186.01.T01:cds;Parent=MsG0880046186.01.T01
Chr8	three_prime_UTR	70255245	70255612	70255245	ID=MsG0880046186.01.T01:three_prime_utr;Parent=MsG0880046186.01.T01

Gene Sequence

>MsG0880046186.01.T01

ATGGGGAGACAACCTTGTTGTGACAAACTTGGTGTGAAGAAAGGTCCATGGACAGCTGAGGAAGACAAGAAACTCATCACTTTTATTCTTACCAATGGACAATGTTGTTGGCGTGCTGTGCCTAAACTTGCCGGCCTTCGGCGCTGCGGTAAGAGCTGTCGTCTTCGTTGGACTAATTATCTTCGTCCTGATTTAAAGAGAGGTCTCCTCACTGAAGCAGAGGAGCAGCTTGTTGTTGATCTCCATGCCCGTCTTGGCAACAGGTGGTCGAAGATTGCTTCACGGTTACCCGGGAGAACAGACAATGAGATTAAAAATCATTGGAACACACACATCAAGAAAAAGCTCATTAAGATGGGAATTGATCCCATTACTCATGAACCTTTGAATAAACAAGCATCATCCAATGATAGTAGTACTTCATCCCCTGCAGAAAATTCGTCACAGCCTGTTAACAACCATGAAGTGAAGGAAACTGATGGGGTTGTCAACTCAGAGGAGAATTCAAGCTCATCCCCAGCTGAAAATTCTTCTGGAGAAGAATCACTTTTGCTAGATAGCATTTGCAGTGACGATTCTCTAATGAATAGTATATGGTTGGATGAAACACCATTGGTGGAAGCTCTTTGGGAAATGGACACTACACCTATAGCAGAAAACACAAAAAATGACATGAGTTTTATGCCTTCTTGGGAGGATAATTGTGCTTGGCTATTTGATTGTCAGGACTTTGGTATTCATGACTTTGGATTTAATTGTTTCAATGAGATAGAGTCAAGTACACTGCAAACTATAGGGATGAAGGAAAATAAACATTAG

Protein sequence

>MsG0880046186.01.T01

MGRQPCCDKLGVKKGPWTAEEDKKLITFILTNGQCCWRAVPKLAGLRRCGKSCRLRWTNYLRPDLKRGLLTEAEEQLVVDLHARLGNRWSKIASRLPGRTDNEIKNHWNTHIKKKLIKMGIDPITHEPLNKQASSNDSSTSSPAENSSQPVNNHEVKETDGVVNSEENSSSSPAENSSGEESLLLDSICSDDSLMNSIWLDETPLVEALWEMDTTPIAENTKNDMSFMPSWEDNCAWLFDCQDFGIHDFGFNCFNEIESSTLQTIGMKENKH*