AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004154.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004154.01.T01 MTR_1g093100 96.269 268 10 0 1 268 169 436 0.0 523
MsG0180004154.01.T01 MTR_1g072420 57.299 274 92 6 1 267 179 434 4.11e-96 289
MsG0180004154.01.T01 MTR_2g022450 55.839 274 98 6 1 267 172 429 5.02e-91 276
MsG0180004154.01.T01 MTR_1g072380 53.214 280 101 6 1 267 188 450 2.54e-90 275
MsG0180004154.01.T01 MTR_1g072450 56.000 275 93 9 1 267 179 433 3.67e-90 274
MsG0180004154.01.T01 MTR_4g117090 50.000 274 109 9 3 262 192 451 7.39e-70 222
MsG0180004154.01.T01 MTR_1g093130 64.024 164 41 2 53 216 9 154 8.64e-61 190
MsG0180004154.01.T01 MTR_2g022320 46.125 271 118 8 1 261 165 417 3.21e-59 194
MsG0180004154.01.T01 MTR_2g022370 44.649 271 121 9 1 263 170 419 3.23e-57 189
MsG0180004154.01.T01 MTR_2g022430 44.361 266 122 8 1 260 175 420 1.38e-56 187
MsG0180004154.01.T01 MTR_2g022460 40.741 270 126 6 1 266 172 411 8.34e-56 185
MsG0180004154.01.T01 MTR_2g022380 43.542 271 124 9 1 263 174 423 6.07e-55 183
MsG0180004154.01.T01 MTR_2g022350 44.195 267 122 9 1 260 174 420 6.38e-53 177
MsG0180004154.01.T01 MTR_2g022490 42.231 251 115 8 1 241 174 404 1.32e-45 158
MsG0180004154.01.T01 MTR_2g022480 41.365 249 116 8 3 241 182 410 3.11e-45 157
MsG0180004154.01.T01 MTR_7g113790 33.449 287 137 11 1 267 184 436 6.27e-35 130
MsG0180004154.01.T01 MTR_0252s0030 38.356 219 111 8 15 219 138 346 1.67e-32 122
MsG0180004154.01.T01 MTR_7g105850 27.757 263 142 12 1 249 232 460 1.03e-12 67.8
MsG0180004154.01.T01 MTR_3g046300 25.592 211 131 6 46 252 268 456 6.30e-12 65.5
MsG0180004154.01.T01 MTR_5g012490 23.741 278 153 10 1 249 217 464 1.26e-11 64.7
MsG0180004154.01.T01 MTR_5g091130 25.197 254 139 11 10 252 338 551 6.16e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004154.01.T01 AT1G03220 51.282 273 111 6 1 267 176 432 2.65e-84 259
MsG0180004154.01.T01 AT1G03230 48.718 273 118 6 1 267 177 433 2.22e-79 246
MsG0180004154.01.T01 AT5G19100 37.751 249 102 8 3 242 161 365 1.52e-40 144
MsG0180004154.01.T01 AT5G19120 36.694 248 111 6 3 249 173 375 8.46e-38 137
MsG0180004154.01.T01 AT5G48430 34.191 272 137 10 1 263 165 403 5.56e-37 135
MsG0180004154.01.T01 AT5G19110 33.333 261 136 9 10 262 171 401 2.82e-33 125
MsG0180004154.01.T01 AT5G19110 33.333 261 136 9 10 262 207 437 5.23e-33 125
MsG0180004154.01.T01 AT3G52500 25.660 265 151 11 1 249 228 462 2.38e-12 67.0
MsG0180004154.01.T01 AT1G09750 27.933 179 115 4 1 178 230 395 4.66e-11 63.2

Find 42 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGAAATCTATTAGAGTTTC+TGG 0.165523 1:-74122328 MsG0180004154.01.T01:CDS
AGGATTAACGGTGCTAATTT+TGG 0.222002 1:+74122246 None:intergenic
AATAAATTTGCAATTTGTTT+AGG 0.256299 1:-74122505 MsG0180004154.01.T01:CDS
ACTGCGCCAAGTTCTTTCTT+AGG 0.277102 1:-74122379 MsG0180004154.01.T01:CDS
TAATTTGTTGCAATTTGATT+TGG 0.302015 1:-74121867 MsG0180004154.01.T01:CDS
TGGTTCCACCAAATCCATTT+TGG 0.303617 1:+74122266 None:intergenic
TCTATAGACCAAAATGGATT+TGG 0.304145 1:-74122274 MsG0180004154.01.T01:CDS
GAACTTGGCGCAGTGCTAAC+AGG 0.339315 1:+74122388 None:intergenic
CGTTAATCCTTACACAATAA+TGG 0.348904 1:-74122233 MsG0180004154.01.T01:CDS
AATGTGGTTTGGAATATTAT+TGG 0.355763 1:-74122046 MsG0180004154.01.T01:CDS
CACTATTGGTGCACATCAAT+TGG 0.356503 1:-74121894 MsG0180004154.01.T01:CDS
TGATAAGGATGTGATTTGTT+TGG 0.378748 1:-74121999 MsG0180004154.01.T01:CDS
GTTGAACCTGTAGCACCATT+TGG 0.387509 1:-74122151 MsG0180004154.01.T01:CDS
CCAATAGTGATTGAAGTCAT+TGG 0.390759 1:+74121908 None:intergenic
CATTATTGTGTAAGGATTAA+CGG 0.392138 1:+74122234 None:intergenic
AAGTATTAGTTCTTCAAGAA+TGG 0.395644 1:-74122110 MsG0180004154.01.T01:CDS
GATAAGGATGTGATTTGTTT+GGG 0.395759 1:-74121998 MsG0180004154.01.T01:CDS
GAAGACTCCATTATTGTGTA+AGG 0.405084 1:+74122226 None:intergenic
CCAATGACTTCAATCACTAT+TGG 0.423745 1:-74121908 MsG0180004154.01.T01:CDS
AAATCAGATGAGTATTTCAT+TGG 0.428391 1:-74122352 MsG0180004154.01.T01:CDS
GTCAAAGGTATGGCTGGTCT+TGG 0.431367 1:-74122580 None:intergenic
AATTTGATTTGGCTACTTCA+AGG 0.431390 1:-74121856 MsG0180004154.01.T01:CDS
AGTATTAGTTCTTCAAGAAT+GGG 0.433655 1:-74122109 MsG0180004154.01.T01:CDS
GATTTGGCTACTTCAAGGCT+TGG 0.435992 1:-74121851 MsG0180004154.01.T01:CDS
CATGTTCCAAATGGTGCTAC+AGG 0.465297 1:+74122145 None:intergenic
CTAACAGGGTTTGTAATAAG+AGG 0.475903 1:+74122403 None:intergenic
AGATCAATGGAAGGAACATC+TGG 0.485151 1:+74122085 None:intergenic
AATGGTTCGTATTAATGATA+AGG 0.488541 1:-74122014 MsG0180004154.01.T01:CDS
ATTCTGCAACACAAGATCAA+TGG 0.498997 1:+74122072 None:intergenic
ATAGACCAAAATGGATTTGG+TGG 0.508375 1:-74122271 MsG0180004154.01.T01:CDS
AAGGGTGTCAAAGGTATGGC+TGG 0.510377 1:-74122586 None:intergenic
TGCAGAATGAGAATGTGGTT+TGG 0.511364 1:-74122057 MsG0180004154.01.T01:CDS
ATTTGTTTAGGTACTCAAGA+TGG 0.523453 1:-74122493 MsG0180004154.01.T01:CDS
TCATCAGTGTTCAATAGATA+AGG 0.532661 1:+74122448 None:intergenic
CTTGCAAAACATGTTCCAAA+TGG 0.563050 1:+74122136 None:intergenic
AACTTGGCGCAGTGCTAACA+GGG 0.570600 1:+74122389 None:intergenic
AAGGTATGGCTGGTCTTGGA+AGG 0.572528 1:-74122576 None:intergenic
AAAGCTGATGAAAATTGTGA+AGG 0.597985 1:+74122538 None:intergenic
TATTATTGGAGCTAATGCAA+TGG 0.621482 1:-74122032 MsG0180004154.01.T01:CDS
TGCAACACAAGATCAATGGA+AGG 0.634453 1:+74122076 None:intergenic
TGTGTTGCAGAATGAGAATG+TGG 0.642355 1:-74122062 MsG0180004154.01.T01:CDS
AATTAAGTTCTAAACAGATG+AGG 0.652970 1:+74121778 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAAATTTGCAATTTGTTT+AGG - Chr1:74121853-74121872 MsG0180004154.01.T01:CDS 15.0%
!!! ATTTTGGTCTATAGATAAAA+GGG + Chr1:74122079-74122098 None:intergenic 20.0%
!!! TAATTTGTTGCAATTTGATT+TGG - Chr1:74122491-74122510 MsG0180004154.01.T01:CDS 20.0%
! AAATCAGATGAGTATTTCAT+TGG - Chr1:74122006-74122025 MsG0180004154.01.T01:CDS 25.0%
! AATGGTTCGTATTAATGATA+AGG - Chr1:74122344-74122363 MsG0180004154.01.T01:CDS 25.0%
! CATTATTGTGTAAGGATTAA+CGG + Chr1:74122127-74122146 None:intergenic 25.0%
!! AAGTATTAGTTCTTCAAGAA+TGG - Chr1:74122248-74122267 MsG0180004154.01.T01:CDS 25.0%
!! AATGTGGTTTGGAATATTAT+TGG - Chr1:74122312-74122331 MsG0180004154.01.T01:CDS 25.0%
!! AGTATTAGTTCTTCAAGAAT+GGG - Chr1:74122249-74122268 MsG0180004154.01.T01:CDS 25.0%
!! CTTTTATCTATAGACCAAAA+TGG - Chr1:74122078-74122097 MsG0180004154.01.T01:CDS 25.0%
!!! CATTTTGGTCTATAGATAAA+AGG + Chr1:74122080-74122099 None:intergenic 25.0%
AAAGCTGATGAAAATTGTGA+AGG + Chr1:74121823-74121842 None:intergenic 30.0%
ATTTGTTTAGGTACTCAAGA+TGG - Chr1:74121865-74121884 MsG0180004154.01.T01:CDS 30.0%
CGTTAATCCTTACACAATAA+TGG - Chr1:74122125-74122144 MsG0180004154.01.T01:CDS 30.0%
GTGAAATCTATTAGAGTTTC+TGG - Chr1:74122030-74122049 MsG0180004154.01.T01:CDS 30.0%
TATTATTGGAGCTAATGCAA+TGG - Chr1:74122326-74122345 MsG0180004154.01.T01:CDS 30.0%
TCATCAGTGTTCAATAGATA+AGG + Chr1:74121913-74121932 None:intergenic 30.0%
TTTGCAAAAACTTCTCAAGT+TGG - Chr1:74122405-74122424 MsG0180004154.01.T01:CDS 30.0%
! TCTATAGACCAAAATGGATT+TGG - Chr1:74122084-74122103 MsG0180004154.01.T01:CDS 30.0%
! TTTTCACCTAAGAAAGAACT+TGG + Chr1:74121988-74122007 None:intergenic 30.0%
!! AATTTGATTTGGCTACTTCA+AGG - Chr1:74122502-74122521 MsG0180004154.01.T01:CDS 30.0%
!! GATAAGGATGTGATTTGTTT+GGG - Chr1:74122360-74122379 MsG0180004154.01.T01:CDS 30.0%
!! TGATAAGGATGTGATTTGTT+TGG - Chr1:74122359-74122378 MsG0180004154.01.T01:CDS 30.0%
ATTCTGCAACACAAGATCAA+TGG + Chr1:74122289-74122308 None:intergenic 35.0%
CCAATAGTGATTGAAGTCAT+TGG + Chr1:74122453-74122472 None:intergenic 35.0%
CCAATGACTTCAATCACTAT+TGG - Chr1:74122450-74122469 MsG0180004154.01.T01:CDS 35.0%
CTAACAGGGTTTGTAATAAG+AGG + Chr1:74121958-74121977 None:intergenic 35.0%
CTTGCAAAACATGTTCCAAA+TGG + Chr1:74122225-74122244 None:intergenic 35.0%
GAAGACTCCATTATTGTGTA+AGG + Chr1:74122135-74122154 None:intergenic 35.0%
! ATAGACCAAAATGGATTTGG+TGG - Chr1:74122087-74122106 MsG0180004154.01.T01:CDS 35.0%
! CAAGATGGTGTTCTTTTCTT+TGG - Chr1:74121880-74121899 MsG0180004154.01.T01:CDS 35.0%
!! AGGATTAACGGTGCTAATTT+TGG + Chr1:74122115-74122134 None:intergenic 35.0%
!!! AACTTCTCAAGTTGGTTTTG+TGG - Chr1:74122413-74122432 MsG0180004154.01.T01:CDS 35.0%
!!! TGTGATTTGTTTGGGTTTTG+TGG - Chr1:74122368-74122387 MsG0180004154.01.T01:CDS 35.0%
AGATCAATGGAAGGAACATC+TGG + Chr1:74122276-74122295 None:intergenic 40.0%
CACTATTGGTGCACATCAAT+TGG - Chr1:74122464-74122483 MsG0180004154.01.T01:CDS 40.0%
TGCAACACAAGATCAATGGA+AGG + Chr1:74122285-74122304 None:intergenic 40.0%
TGCAGAATGAGAATGTGGTT+TGG - Chr1:74122301-74122320 MsG0180004154.01.T01:CDS 40.0%
TGTGTTGCAGAATGAGAATG+TGG - Chr1:74122296-74122315 MsG0180004154.01.T01:CDS 40.0%
! GGTGTTCTTTTCTTTGGTGA+TGG - Chr1:74121886-74121905 MsG0180004154.01.T01:CDS 40.0%
! TGGTTCCACCAAATCCATTT+TGG + Chr1:74122095-74122114 None:intergenic 40.0%
!! CTACTTCAAGGCTTGGTTTT+AGG - Chr1:74122514-74122533 MsG0180004154.01.T01:CDS 40.0%
!!! TGGTTTTAGGTCACTCTTCT+TGG - Chr1:74122527-74122546 MsG0180004154.01.T01:CDS 40.0%
ACTGCGCCAAGTTCTTTCTT+AGG - Chr1:74121979-74121998 MsG0180004154.01.T01:CDS 45.0%
GATTTGGCTACTTCAAGGCT+TGG - Chr1:74122507-74122526 MsG0180004154.01.T01:CDS 45.0%
GTTGAACCTGTAGCACCATT+TGG - Chr1:74122207-74122226 MsG0180004154.01.T01:CDS 45.0%
!! CATGTTCCAAATGGTGCTAC+AGG + Chr1:74122216-74122235 None:intergenic 45.0%
!! TGTTTGGGTTTTGTGGATGC+TGG - Chr1:74122375-74122394 MsG0180004154.01.T01:CDS 45.0%
!!! TCTCAAGTTGGTTTTGTGGC+TGG - Chr1:74122417-74122436 MsG0180004154.01.T01:CDS 45.0%
AACTTGGCGCAGTGCTAACA+GGG + Chr1:74121972-74121991 None:intergenic 50.0%
!!! CAAGTTGGTTTTGTGGCTGG+TGG - Chr1:74122420-74122439 MsG0180004154.01.T01:CDS 50.0%
GAACTTGGCGCAGTGCTAAC+AGG + Chr1:74121973-74121992 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 74121787 74122593 74121787 ID=MsG0180004154.01;Name=MsG0180004154.01
Chr1 mRNA 74121787 74122593 74121787 ID=MsG0180004154.01.T01;Parent=MsG0180004154.01;Name=MsG0180004154.01.T01;_AED=0.26;_eAED=0.26;_QI=0|-1|0|1|-1|1|1|0|268
Chr1 exon 74121787 74122593 74121787 ID=MsG0180004154.01.T01:exon:5515;Parent=MsG0180004154.01.T01
Chr1 CDS 74121787 74122593 74121787 ID=MsG0180004154.01.T01:cds;Parent=MsG0180004154.01.T01
Gene Sequence

>MsG0180004154.01.T01

ATGGCTGGTCTTGGAAGGACTAAAGTGTCACTTCCTTCACAATTTTCATCAGCTTTTAGTTTCAAGAATAAATTTGCAATTTGTTTAGGTACTCAAGATGGTGTTCTTTTCTTTGGTGATGGACCTTATCTATTGAACACTGATGAATCTAAAAATCTTATTTTCACACCTCTTATTACAAACCCTGTTAGCACTGCGCCAAGTTCTTTCTTAGGTGAAAAATCAGATGAGTATTTCATTGGTGTGAAATCTATTAGAGTTTCTGGTAAAAATGTAAAGTTGAACACAACCCTTTTATCTATAGACCAAAATGGATTTGGTGGAACCAAAATTAGCACCGTTAATCCTTACACAATAATGGAGTCTTCAATTTATAAAGCAGTTGCTGATGCTTTTGTGAAAGCATTAAATGTTTCTACTGTTGAACCTGTAGCACCATTTGGAACATGTTTTGCAAGTCAAAGTATTAGTTCTTCAAGAATGGGACCAGATGTTCCTTCCATTGATCTTGTGTTGCAGAATGAGAATGTGGTTTGGAATATTATTGGAGCTAATGCAATGGTTCGTATTAATGATAAGGATGTGATTTGTTTGGGTTTTGTGGATGCTGGTTCTGATTTTGCAAAAACTTCTCAAGTTGGTTTTGTGGCTGGTGGTTCTAAACCAATGACTTCAATCACTATTGGTGCACATCAATTGGAGAATAATTTGTTGCAATTTGATTTGGCTACTTCAAGGCTTGGTTTTAGGTCACTCTTCTTGGAGCATGATAATTGTGCAAACTTTAATTTTACCTCATCTGTTTAG

Protein sequence

>MsG0180004154.01.T01

MAGLGRTKVSLPSQFSSAFSFKNKFAICLGTQDGVLFFGDGPYLLNTDESKNLIFTPLITNPVSTAPSSFLGEKSDEYFIGVKSIRVSGKNVKLNTTLLSIDQNGFGGTKISTVNPYTIMESSIYKAVADAFVKALNVSTVEPVAPFGTCFASQSISSSRMGPDVPSIDLVLQNENVVWNIIGANAMVRINDKDVICLGFVDAGSDFAKTSQVGFVAGGSKPMTSITIGAHQLENNLLQFDLATSRLGFRSLFLEHDNCANFNFTSSV*