AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007934.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007934.01.T01 MTR_2g435490 98.361 183 3 0 16 198 1 183 8.02e-138 382
MsG0280007934.01.T01 MTR_4g050762 58.599 157 60 2 35 189 16 169 2.89e-66 201
MsG0280007934.01.T01 MTR_2g012370 58.599 157 60 2 35 189 16 169 2.89e-66 201
MsG0280007934.01.T01 MTR_2g010600 56.962 158 65 2 24 179 6 162 7.52e-66 200
MsG0280007934.01.T01 MTR_2g010590 53.750 160 73 1 20 179 1 159 2.39e-62 191
MsG0280007934.01.T01 MTR_2g010580 51.572 159 73 3 23 179 8 164 2.02e-57 178
MsG0280007934.01.T01 MTR_2g010630 55.844 154 60 4 32 179 14 165 5.80e-57 177
MsG0280007934.01.T01 MTR_2g010650 54.118 170 67 7 16 179 1 165 8.68e-56 174
MsG0280007934.01.T01 MTR_2g010610 50.345 145 50 2 35 179 16 138 9.41e-50 158
MsG0280007934.01.T01 MTR_4g092540 45.912 159 82 4 22 179 4 159 1.45e-48 155
MsG0280007934.01.T01 MTR_4g128750 49.650 143 70 2 39 179 31 173 2.55e-46 150
MsG0280007934.01.T01 MTR_2g010700 46.341 164 77 5 25 179 7 168 6.16e-46 149
MsG0280007934.01.T01 MTR_2g010640 46.584 161 78 5 24 179 6 163 1.94e-44 145
MsG0280007934.01.T01 MTR_2g010690 44.444 162 81 5 24 179 6 164 1.16e-40 136
MsG0280007934.01.T01 MTR_2g010670 45.062 162 80 5 24 179 6 164 2.09e-39 132
MsG0280007934.01.T01 MTR_4g128770 44.203 138 71 3 47 179 70 206 3.55e-38 130
MsG0280007934.01.T01 MTR_8g078770 37.143 140 84 3 44 179 33 172 1.28e-32 115
MsG0280007934.01.T01 MTR_1g067290 38.235 136 81 3 47 179 73 208 6.34e-31 112
MsG0280007934.01.T01 MTR_5g018770 37.857 140 81 4 46 179 46 185 7.86e-31 111
MsG0280007934.01.T01 MTR_4g092515 51.136 88 42 1 24 110 9 96 3.72e-23 90.1
MsG0280007934.01.T01 MTR_5g018755 35.165 91 54 4 94 179 13 103 5.27e-12 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007934.01.T01 AT2G14610 57.595 158 64 2 22 179 7 161 1.66e-68 206
MsG0280007934.01.T01 AT4G33720 56.688 157 66 2 23 179 9 163 1.87e-67 204
MsG0280007934.01.T01 AT2G14580 58.228 158 63 2 22 179 7 161 1.96e-67 204
MsG0280007934.01.T01 AT3G19690 51.220 164 74 3 18 179 2 161 7.93e-58 179
MsG0280007934.01.T01 AT4G33710 55.319 141 60 2 41 179 27 166 4.59e-53 167
MsG0280007934.01.T01 AT5G26130 50.633 158 74 3 24 179 11 166 2.09e-52 166
MsG0280007934.01.T01 AT4G33730 51.282 156 73 3 25 179 19 172 3.17e-52 166
MsG0280007934.01.T01 AT2G19990 53.333 135 63 0 45 179 42 176 3.70e-52 166
MsG0280007934.01.T01 AT4G25790 49.630 135 67 1 46 179 76 210 3.85e-49 159
MsG0280007934.01.T01 AT1G50060 50.000 154 75 2 27 179 9 161 2.44e-48 155
MsG0280007934.01.T01 AT5G57625 50.376 133 65 1 48 179 75 207 1.93e-47 154
MsG0280007934.01.T01 AT4G30320 43.709 151 80 3 30 179 15 161 7.34e-45 146
MsG0280007934.01.T01 AT3G09590 40.764 157 91 1 25 179 30 186 1.65e-43 144
MsG0280007934.01.T01 AT1G50050 47.742 155 78 3 27 179 9 162 2.04e-41 138
MsG0280007934.01.T01 AT1G01310 41.830 153 74 3 47 198 87 225 1.19e-40 138
MsG0280007934.01.T01 AT4G31470 44.444 135 73 2 46 179 52 185 2.21e-38 130
MsG0280007934.01.T01 AT4G25780 43.066 137 72 4 48 179 55 190 4.18e-37 127
MsG0280007934.01.T01 AT1G50050 47.586 145 73 3 27 169 9 152 4.62e-37 129
MsG0280007934.01.T01 AT5G02730 39.583 144 85 1 47 188 59 202 2.43e-36 126
MsG0280007934.01.T01 AT4G07820 42.446 139 77 3 41 179 25 160 7.98e-35 121
MsG0280007934.01.T01 AT5G66590 39.286 140 78 3 46 179 47 185 1.19e-30 111
MsG0280007934.01.T01 AT2G19970 40.299 134 69 5 45 169 35 166 5.22e-26 99.0
MsG0280007934.01.T01 AT2G19980 35.542 166 84 8 27 179 10 165 1.84e-22 89.4

Find 45 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACATTATACTCAGGTTGTT+TGG 0.338106 2:+23247767 MsG0280007934.01.T01:CDS
GAAGGAGATGTGAGATCATT+GGG 0.354867 2:-23247417 None:intergenic
ATTAAAAGCAAACTGCAAAA+TGG 0.363614 2:+23247603 MsG0280007934.01.T01:CDS
GATCCACCTGGTAATTATAT+TGG 0.367528 2:+23247865 MsG0280007934.01.T01:CDS
TTGTTTGGCGCGATTCGGTT+CGG 0.384031 2:+23247782 MsG0280007934.01.T01:CDS
TCCCATGTTGAGAAAAGCTT+TGG 0.385556 2:-23247392 None:intergenic
TTGGTGTTGGCCCAATATCT+TGG 0.387017 2:+23247539 MsG0280007934.01.T01:CDS
TGTTTGGCGCGATTCGGTTC+GGG 0.388041 2:+23247783 MsG0280007934.01.T01:CDS
TCAGGTTGTTTGGCGCGATT+CGG 0.394956 2:+23247777 MsG0280007934.01.T01:CDS
ATCATAGTTACAACTAATAA+TGG 0.408982 2:-23247845 None:intergenic
GGAAGGAGATGTGAGATCAT+TGG 0.420420 2:-23247418 None:intergenic
AAGCTCAATGGCACTTCAAA+AGG 0.430809 2:-23247910 None:intergenic
TGGTGTTGGCCCAATATCTT+GGG 0.465713 2:+23247540 MsG0280007934.01.T01:CDS
GTACACTCCAAGGGTCCATA+TGG 0.472690 2:+23247625 MsG0280007934.01.T01:CDS
AACAAAGCACGCTCTGACGT+TGG 0.488151 2:+23247520 MsG0280007934.01.T01:CDS
TCAAAAGGACTAATATCAAA+AGG 0.488505 2:-23247895 None:intergenic
AACAGTAATTCATGTGCTGT+TGG 0.492187 2:+23247733 MsG0280007934.01.T01:CDS
CTCACATCTCCTTCCACTCA+TGG 0.495622 2:+23247426 MsG0280007934.01.T01:CDS
CTACCATGGTTGAAGCTCAA+TGG 0.504277 2:-23247922 None:intergenic
CTGCAAAATGGTACACTCCA+AGG 0.542543 2:+23247615 MsG0280007934.01.T01:CDS
GCACGCTCTGACGTTGGTGT+TGG 0.542698 2:+23247526 MsG0280007934.01.T01:CDS
CTTTGACCAATATAATTACC+AGG 0.549756 2:-23247871 None:intergenic
ACAAGATAGCCATGAGTGGA+AGG 0.552738 2:-23247435 None:intergenic
TGGCGCGATTCGGTTCGGGT+TGG 0.564234 2:+23247787 MsG0280007934.01.T01:CDS
TGGAGCAGTGGTGACATGAC+AGG 0.569134 2:+23247661 MsG0280007934.01.T01:CDS
GGTTCGGGTTGGATGTGCTA+AGG 0.572627 2:+23247798 MsG0280007934.01.T01:CDS
GCTGCTGTTACCATGTGGAT+TGG 0.573532 2:+23247688 MsG0280007934.01.T01:CDS
CTGGTAATTATATTGGTCAA+AGG 0.577693 2:+23247872 MsG0280007934.01.T01:CDS
GTTTACATAGGTTTGTGCAT+AGG 0.587350 2:-23247578 None:intergenic
GAAAATTTAGCATGGAGCAG+TGG 0.597306 2:+23247649 MsG0280007934.01.T01:CDS
GCTAAGGTAAAATGTAATGA+TGG 0.604315 2:+23247814 MsG0280007934.01.T01:CDS
TGAATCTTGATGTAGTCTTG+TGG 0.606016 2:-23247496 None:intergenic
TGCAAAATGGTACACTCCAA+GGG 0.616230 2:+23247616 MsG0280007934.01.T01:CDS
CATATGGAGAAAATTTAGCA+TGG 0.617244 2:+23247641 MsG0280007934.01.T01:CDS
ACTTGTCATCAAAACTACCA+TGG 0.632716 2:-23247936 None:intergenic
TGTGCTGTTGGTTATCAGTG+TGG 0.633545 2:+23247745 MsG0280007934.01.T01:CDS
GTGCCATTGAGCTTCAACCA+TGG 0.637543 2:+23247919 MsG0280007934.01.T01:CDS
AAATGAGATTTGTGTTAGTG+TGG 0.643782 2:-23247461 None:intergenic
TCAGTGTGGACATTATACTC+AGG 0.656601 2:+23247759 MsG0280007934.01.T01:CDS
TTTCTTCTCTCCAATCCACA+TGG 0.658038 2:-23247698 None:intergenic
AGTTGTAACTATGATCCACC+TGG 0.667304 2:+23247853 MsG0280007934.01.T01:CDS
GCTAACAAGATAGCCATGAG+TGG 0.671580 2:-23247439 None:intergenic
GAACAGCTGCTGTTACCATG+TGG 0.688628 2:+23247683 MsG0280007934.01.T01:CDS
TGACCAATATAATTACCAGG+TGG 0.723440 2:-23247868 None:intergenic
AAGGAGATGTGAGATCATTG+GGG 0.724704 2:-23247416 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCATAGTTACAACTAATAA+TGG - Chr2:23247848-23247867 None:intergenic 20.0%
!!! TGCTTTTAATTTGTTTACAT+AGG - Chr2:23247593-23247612 None:intergenic 20.0%
! ATTAAAAGCAAACTGCAAAA+TGG + Chr2:23247603-23247622 MsG0280007934.01.T01:CDS 25.0%
! TCAAAAGGACTAATATCAAA+AGG - Chr2:23247898-23247917 None:intergenic 25.0%
AAATGAGATTTGTGTTAGTG+TGG - Chr2:23247464-23247483 None:intergenic 30.0%
CATATGGAGAAAATTTAGCA+TGG + Chr2:23247641-23247660 MsG0280007934.01.T01:CDS 30.0%
CTGGTAATTATATTGGTCAA+AGG + Chr2:23247872-23247891 MsG0280007934.01.T01:CDS 30.0%
CTTTGACCAATATAATTACC+AGG - Chr2:23247874-23247893 None:intergenic 30.0%
GCTAAGGTAAAATGTAATGA+TGG + Chr2:23247814-23247833 MsG0280007934.01.T01:CDS 30.0%
! CATGCTAAATTTTCTCCATA+TGG - Chr2:23247643-23247662 None:intergenic 30.0%
!!! AAAGCTTTGGTTTTGATAGA+TGG - Chr2:23247382-23247401 None:intergenic 30.0%
AACAGTAATTCATGTGCTGT+TGG + Chr2:23247733-23247752 MsG0280007934.01.T01:CDS 35.0%
ACTTGTCATCAAAACTACCA+TGG - Chr2:23247939-23247958 None:intergenic 35.0%
GATCCACCTGGTAATTATAT+TGG + Chr2:23247865-23247884 MsG0280007934.01.T01:CDS 35.0%
GTTTACATAGGTTTGTGCAT+AGG - Chr2:23247581-23247600 None:intergenic 35.0%
TGACCAATATAATTACCAGG+TGG - Chr2:23247871-23247890 None:intergenic 35.0%
! AACCAAAGCTTTTCTCAACA+TGG + Chr2:23247390-23247409 MsG0280007934.01.T01:CDS 35.0%
! ACCAAAGCTTTTCTCAACAT+GGG + Chr2:23247391-23247410 MsG0280007934.01.T01:CDS 35.0%
! ACTTTTGCATCCCAAGATAT+TGG - Chr2:23247553-23247572 None:intergenic 35.0%
! CTTTTGCATCCCAAGATATT+GGG - Chr2:23247552-23247571 None:intergenic 35.0%
! GACATTATACTCAGGTTGTT+TGG + Chr2:23247767-23247786 MsG0280007934.01.T01:CDS 35.0%
! TGAATCTTGATGTAGTCTTG+TGG - Chr2:23247499-23247518 None:intergenic 35.0%
AAGCTCAATGGCACTTCAAA+AGG - Chr2:23247913-23247932 None:intergenic 40.0%
AAGGAGATGTGAGATCATTG+GGG - Chr2:23247419-23247438 None:intergenic 40.0%
AGTTGTAACTATGATCCACC+TGG + Chr2:23247853-23247872 MsG0280007934.01.T01:CDS 40.0%
GAAAATTTAGCATGGAGCAG+TGG + Chr2:23247649-23247668 MsG0280007934.01.T01:CDS 40.0%
GAAGGAGATGTGAGATCATT+GGG - Chr2:23247420-23247439 None:intergenic 40.0%
TCAGTGTGGACATTATACTC+AGG + Chr2:23247759-23247778 MsG0280007934.01.T01:CDS 40.0%
TCCCATGTTGAGAAAAGCTT+TGG - Chr2:23247395-23247414 None:intergenic 40.0%
TGCAAAATGGTACACTCCAA+GGG + Chr2:23247616-23247635 MsG0280007934.01.T01:CDS 40.0%
TTTCTTCTCTCCAATCCACA+TGG - Chr2:23247701-23247720 None:intergenic 40.0%
! ATTTTCTCCATATGGACCCT+TGG - Chr2:23247635-23247654 None:intergenic 40.0%
!!! TGGTTTTGATAGATGGTGCT+TGG - Chr2:23247375-23247394 None:intergenic 40.0%
ACAAGATAGCCATGAGTGGA+AGG - Chr2:23247438-23247457 None:intergenic 45.0%
CTACCATGGTTGAAGCTCAA+TGG - Chr2:23247925-23247944 None:intergenic 45.0%
CTGCAAAATGGTACACTCCA+AGG + Chr2:23247615-23247634 MsG0280007934.01.T01:CDS 45.0%
GCTAACAAGATAGCCATGAG+TGG - Chr2:23247442-23247461 None:intergenic 45.0%
GGAAGGAGATGTGAGATCAT+TGG - Chr2:23247421-23247440 None:intergenic 45.0%
! TGTGCTGTTGGTTATCAGTG+TGG + Chr2:23247745-23247764 MsG0280007934.01.T01:CDS 45.0%
!! TGGTGTTGGCCCAATATCTT+GGG + Chr2:23247540-23247559 MsG0280007934.01.T01:CDS 45.0%
!! TTGGTGTTGGCCCAATATCT+TGG + Chr2:23247539-23247558 MsG0280007934.01.T01:CDS 45.0%
AACAAAGCACGCTCTGACGT+TGG + Chr2:23247520-23247539 MsG0280007934.01.T01:CDS 50.0%
CTCACATCTCCTTCCACTCA+TGG + Chr2:23247426-23247445 MsG0280007934.01.T01:CDS 50.0%
GAACAGCTGCTGTTACCATG+TGG + Chr2:23247683-23247702 MsG0280007934.01.T01:CDS 50.0%
GCTGCTGTTACCATGTGGAT+TGG + Chr2:23247688-23247707 MsG0280007934.01.T01:CDS 50.0%
GTACACTCCAAGGGTCCATA+TGG + Chr2:23247625-23247644 MsG0280007934.01.T01:CDS 50.0%
! GTGCCATTGAGCTTCAACCA+TGG + Chr2:23247919-23247938 MsG0280007934.01.T01:CDS 50.0%
! TCAGGTTGTTTGGCGCGATT+CGG + Chr2:23247777-23247796 MsG0280007934.01.T01:CDS 50.0%
!! TTGTTTGGCGCGATTCGGTT+CGG + Chr2:23247782-23247801 MsG0280007934.01.T01:CDS 50.0%
TGGAGCAGTGGTGACATGAC+AGG + Chr2:23247661-23247680 MsG0280007934.01.T01:CDS 55.0%
!! GGTTCGGGTTGGATGTGCTA+AGG + Chr2:23247798-23247817 MsG0280007934.01.T01:CDS 55.0%
!! TGTTTGGCGCGATTCGGTTC+GGG + Chr2:23247783-23247802 MsG0280007934.01.T01:CDS 55.0%
GCACGCTCTGACGTTGGTGT+TGG + Chr2:23247526-23247545 MsG0280007934.01.T01:CDS 60.0%
!! TGGCGCGATTCGGTTCGGGT+TGG + Chr2:23247787-23247806 MsG0280007934.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 23247364 23247960 23247364 ID=MsG0280007934.01;Name=MsG0280007934.01
Chr2 mRNA 23247364 23247960 23247364 ID=MsG0280007934.01.T01;Parent=MsG0280007934.01;Name=MsG0280007934.01.T01;_AED=0.28;_eAED=0.28;_QI=0|-1|0|1|-1|1|1|0|198
Chr2 exon 23247364 23247960 23247364 ID=MsG0280007934.01.T01:exon:18433;Parent=MsG0280007934.01.T01
Chr2 CDS 23247364 23247960 23247364 ID=MsG0280007934.01.T01:cds;Parent=MsG0280007934.01.T01
Gene Sequence

>MsG0280007934.01.T01

ATGACTCACCAAGCACCATCTATCAAAACCAAAGCTTTTCTCAACATGGGATCCCCAATGATCTCACATCTCCTTCCACTCATGGCTATCTTGTTAGCCACACTAACACAAATCTCATTTGCTCAAAACTCACCACAAGACTACATCAAGATTCACAACAAAGCACGCTCTGACGTTGGTGTTGGCCCAATATCTTGGGATGCAAAAGTTGCTTCCTATGCACAAACCTATGTAAACAAATTAAAAGCAAACTGCAAAATGGTACACTCCAAGGGTCCATATGGAGAAAATTTAGCATGGAGCAGTGGTGACATGACAGGAACAGCTGCTGTTACCATGTGGATTGGAGAGAAGAAATACTATAATTACAACAGTAATTCATGTGCTGTTGGTTATCAGTGTGGACATTATACTCAGGTTGTTTGGCGCGATTCGGTTCGGGTTGGATGTGCTAAGGTAAAATGTAATGATGGTCGTAGCACCATTATTAGTTGTAACTATGATCCACCTGGTAATTATATTGGTCAAAGGCCTTTTGATATTAGTCCTTTTGAAGTGCCATTGAGCTTCAACCATGGTAGTTTTGATGACAAGTAA

Protein sequence

>MsG0280007934.01.T01

MTHQAPSIKTKAFLNMGSPMISHLLPLMAILLATLTQISFAQNSPQDYIKIHNKARSDVGVGPISWDAKVASYAQTYVNKLKANCKMVHSKGPYGENLAWSSGDMTGTAAVTMWIGEKKYYNYNSNSCAVGYQCGHYTQVVWRDSVRVGCAKVKCNDGRSTIISCNYDPPGNYIGQRPFDISPFEVPLSFNHGSFDDK*