AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280009299.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009299.01.T01 MTR_6g090515 91.729 133 11 0 104 236 88 220 1.29e-63 197
MsG0280009299.01.T01 MTR_8g102030 79.699 133 25 2 104 236 92 222 7.44e-46 152
MsG0280009299.01.T01 MTR_8g102020 63.910 133 47 1 104 236 74 205 1.39e-41 141
MsG0280009299.01.T01 MTR_7g089090 89.474 76 8 0 34 109 169 244 1.33e-37 137
MsG0280009299.01.T01 MTR_5g088860 56.579 76 33 0 34 109 168 243 1.14e-19 87.4
MsG0280009299.01.T01 MTR_5g088860 56.579 76 33 0 34 109 190 265 1.17e-19 87.4
MsG0280009299.01.T01 MTR_3g045180 53.947 76 35 0 34 109 164 239 7.52e-18 82.4
MsG0280009299.01.T01 MTR_6g079040 82.222 45 7 1 33 77 119 162 1.16e-14 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009299.01.T01 AT4G29160 80.597 134 25 1 104 236 59 192 1.61e-55 176
MsG0280009299.01.T01 AT4G29160 80.597 134 25 1 104 236 86 219 3.84e-55 176
MsG0280009299.01.T01 AT4G29160 80.597 134 25 1 104 236 86 219 3.84e-55 176
MsG0280009299.01.T01 AT2G19830 77.612 134 22 3 104 236 68 194 2.21e-50 163
MsG0280009299.01.T01 AT2G19830 77.612 134 22 3 104 236 87 213 2.45e-50 164
MsG0280009299.01.T01 AT2G40820 65.385 78 25 1 34 109 61 138 1.61e-24 100
MsG0280009299.01.T01 AT3G56480 62.821 78 27 1 34 109 168 245 1.64e-24 101
MsG0280009299.01.T01 AT2G40820 65.385 78 25 1 34 109 183 260 4.26e-24 100
MsG0280009299.01.T01 AT2G40820 65.385 78 25 1 34 109 171 248 4.46e-24 100
MsG0280009299.01.T01 AT2G40820 65.385 78 25 1 34 109 171 248 4.46e-24 100
MsG0280009299.01.T01 AT2G40820 65.385 78 25 1 34 109 196 273 4.84e-24 100
MsG0280009299.01.T01 AT2G26770 57.895 76 32 0 34 109 168 243 1.17e-19 87.8
MsG0280009299.01.T01 AT2G26770 57.895 76 32 0 34 109 168 243 1.17e-19 87.8
MsG0280009299.01.T01 AT2G26770 57.895 76 32 0 34 109 168 243 1.17e-19 87.8

Find 69 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTCAGGCGTCGGCTTTGC+AGG 0.230011 2:-49733904 None:intergenic
AGAAATCTGCTTCTTCCTTC+TGG 0.259872 2:+49734249 MsG0280009299.01.T01:three_prime_UTR
TAACAAAACCAGCGAAATTC+TGG 0.276169 2:-49734167 None:intergenic
AGAGCAGTGGTGCTGGGAAT+TGG 0.301411 2:+49729841 MsG0280009299.01.T01:CDS
CCCACAGTGCATGTCTCAGC+TGG 0.318746 2:+49733859 MsG0280009299.01.T01:CDS
GAGCAGTGGTGCTGGGAATT+GGG 0.318997 2:+49729842 MsG0280009299.01.T01:CDS
ACAACAATCTAAAATAATTC+TGG 0.328240 2:-49734109 None:intergenic
GGAAGCAGAACTTGAAGAAT+TGG 0.342673 2:+49733783 MsG0280009299.01.T01:CDS
AGGCTTCTGAAGATGCTAAA+AGG 0.361461 2:+49729770 MsG0280009299.01.T01:intron
TGCTCTAGCACTCTCGATTT+CGG 0.373790 2:-49729824 None:intergenic
GACATCAATGTATCAATTAA+CGG 0.374331 2:-49734064 None:intergenic
AGATGAATTGGCAGCTCTGC+AGG 0.394946 2:+49733927 MsG0280009299.01.T01:CDS
ATTAAGCAATGTACCTTACT+TGG 0.401978 2:-49730188 None:intergenic
AAATAAGCAGCTGCACCAAT+TGG 0.420089 2:-49733320 None:intergenic
GACGCCTGAGGAAGATGAAT+TGG 0.434096 2:+49733915 MsG0280009299.01.T01:CDS
AACTTGATATGGCAAGCTTA+AGG 0.434801 2:+49729573 None:intergenic
TGGCAAGCTTAAGGTGGTAT+AGG 0.439585 2:+49729582 MsG0280009299.01.T01:CDS
ATAGTAGATGCGTTAAGAAC+AGG 0.445629 2:+49733077 MsG0280009299.01.T01:CDS
GAGTGCTAGAGCAGTGGTGC+TGG 0.451158 2:+49729834 MsG0280009299.01.T01:CDS
GGCGTCGGCTTTGCAGGAAC+AGG 0.452744 2:-49733898 None:intergenic
TAATAGATGATAATGTTAGA+AGG 0.453601 2:+49733035 MsG0280009299.01.T01:intron
TACACAAGCTTCTAAACCAC+AGG 0.464474 2:+49729891 MsG0280009299.01.T01:CDS
GGCAGCTCTGCAGGCTGAGA+TGG 0.469864 2:+49733936 MsG0280009299.01.T01:CDS
AAATCTGTCGGTTGTGTCAC+GGG 0.480701 2:-49733984 None:intergenic
AGCTTAAGCCAGAATTTCGC+TGG 0.481728 2:+49734159 MsG0280009299.01.T01:three_prime_UTR
TGTGAAATTATTGAATAGAC+TGG 0.484250 2:+49734281 MsG0280009299.01.T01:three_prime_UTR
TTTGCAGGAACAGGACGTGC+AGG 0.493724 2:-49733889 None:intergenic
TTCTGAAGATGCTAAAAGGT+TGG 0.504212 2:+49729774 MsG0280009299.01.T01:CDS
CCAGCTGAGACATGCACTGT+GGG 0.507614 2:-49733859 None:intergenic
CTGTCAGGATGTGGATGGAC+TGG 0.508897 2:+49730121 MsG0280009299.01.T01:intron
GAGACATGCACTGTGGGAGC+AGG 0.513136 2:-49733853 None:intergenic
GAAGCATTGTCAGCTCCAAT+TGG 0.513583 2:+49733305 MsG0280009299.01.T01:CDS
TAAGGTGGTATAGGTACAAA+GGG 0.516032 2:+49729591 MsG0280009299.01.T01:CDS
AGAATTGGAGAATGCTGAGT+TGG 0.518601 2:+49733798 MsG0280009299.01.T01:CDS
CTGTTCATTTATCTCGTCCA+TGG 0.520922 2:-49733258 None:intergenic
AGCAGTGGTGCTGGGAATTG+GGG 0.521566 2:+49729843 MsG0280009299.01.T01:CDS
ATTGCTGCATCAGCCAAGTA+AGG 0.523393 2:+49730175 MsG0280009299.01.T01:CDS
TCTTACTGTCAGGATGTGGA+TGG 0.524962 2:+49730116 MsG0280009299.01.T01:intron
CCACAGTGCATGTCTCAGCT+GGG 0.525773 2:+49733860 MsG0280009299.01.T01:CDS
GCTGCCAATTCATCTTCCTC+AGG 0.527733 2:-49733919 None:intergenic
AACAGGAGCAACTACTATGA+AGG 0.534991 2:+49733094 MsG0280009299.01.T01:CDS
GCTGAGATGGCACTTTGAGC+GGG 0.538605 2:+49733949 MsG0280009299.01.T01:exon
TGTGGATGGACTGGTGGAAG+AGG 0.543368 2:+49730130 MsG0280009299.01.T01:CDS
CGCATCTACTATTTCCGTGG+TGG 0.544524 2:-49733066 None:intergenic
CAAATCTGTCGGTTGTGTCA+CGG 0.546560 2:-49733985 None:intergenic
GGCTGAGATGGCACTTTGAG+CGG 0.549673 2:+49733948 MsG0280009299.01.T01:exon
TTAAGGTGGTATAGGTACAA+AGG 0.559744 2:+49729590 MsG0280009299.01.T01:CDS
GATAAATAACACAAATCTGT+CGG 0.566709 2:-49733996 None:intergenic
TAACGCATCTACTATTTCCG+TGG 0.566786 2:-49733069 None:intergenic
GTCTAAACAATCTGAACCAA+AGG 0.568231 2:+49729661 MsG0280009299.01.T01:CDS
GTACAAATTACAATATGTCA+TGG 0.571545 2:-49734210 None:intergenic
AGTGCTAGAGCAGTGGTGCT+GGG 0.572493 2:+49729835 MsG0280009299.01.T01:CDS
TCCTGCAAAGCCGACGCCTG+AGG 0.582259 2:+49733903 MsG0280009299.01.T01:CDS
AGGTACAAAGGGAATGATGA+TGG 0.582511 2:+49729602 MsG0280009299.01.T01:CDS
AATTCATCTTCCTCAGGCGT+CGG 0.583818 2:-49733913 None:intergenic
GATGGTACTGATCAAGTTAG+TGG 0.592941 2:+49729620 MsG0280009299.01.T01:CDS
TCAGGATGTGGATGGACTGG+TGG 0.623988 2:+49730124 MsG0280009299.01.T01:intron
AGTGGTGCTGGGAATTGGGG+AGG 0.632259 2:+49729846 MsG0280009299.01.T01:CDS
CCCAGCTGAGACATGCACTG+TGG 0.640471 2:-49733860 None:intergenic
TTCACACATACAAAGCCAGA+AGG 0.641929 2:-49734264 None:intergenic
ATGGAAGACACTTATCGTCA+CGG 0.650185 2:-49734191 None:intergenic
TTGATATGGCAAGCTTAAGG+TGG 0.651613 2:+49729576 None:intergenic
TGATGATGTCGACAAGACCA+TGG 0.653403 2:+49733241 MsG0280009299.01.T01:CDS
ACTGGTGGAAGAGGTTCAAG+AGG 0.653599 2:+49730139 MsG0280009299.01.T01:CDS
AATCGAGAGTGCTAGAGCAG+TGG 0.666858 2:+49729828 MsG0280009299.01.T01:CDS
TTTGTACATAATGACTGATG+TGG 0.669623 2:+49734226 MsG0280009299.01.T01:three_prime_UTR
GGAGCAGGAGCTGTAAATGT+TGG 0.671498 2:-49733838 None:intergenic
CAGTGCATGTCTCAGCTGGG+AGG 0.674111 2:+49733863 MsG0280009299.01.T01:CDS
AGAAGGTGCTAAAGCCACCA+CGG 0.700426 2:+49733052 MsG0280009299.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTATTTTTTGAACAAAA+TGG - Chr2:49732639-49732658 None:intergenic 10.0%
!! TGAAATAATAACATTAAACA+GGG + Chr2:49733594-49733613 MsG0280009299.01.T01:intron 15.0%
!! TTGAAATAATAACATTAAAC+AGG + Chr2:49733593-49733612 MsG0280009299.01.T01:intron 15.0%
!! TTTCATTTATATATTCACAT+TGG + Chr2:49730257-49730276 MsG0280009299.01.T01:intron 15.0%
!!! ATTTGTCTTATTTTTTTGTT+TGG + Chr2:49731401-49731420 MsG0280009299.01.T01:intron 15.0%
!! AAAATAACCAACGATATTAT+TGG - Chr2:49731571-49731590 None:intergenic 20.0%
!! AAACAAGATATTTGATTATG+TGG + Chr2:49733391-49733410 MsG0280009299.01.T01:intron 20.0%
!! AATTTAAATCACCTGTTATT+GGG - Chr2:49732423-49732442 None:intergenic 20.0%
!! ATATATGTTGAACTTTATGT+GGG + Chr2:49731022-49731041 MsG0280009299.01.T01:intron 20.0%
!! GAACAAAAATATAGATCATA+TGG - Chr2:49730864-49730883 None:intergenic 20.0%
!! GTGATATATTTAATTGAGTT+TGG + Chr2:49733194-49733213 MsG0280009299.01.T01:intron 20.0%
!! TAAAATTGATACAATGTTGT+TGG + Chr2:49731818-49731837 MsG0280009299.01.T01:intron 20.0%
!! TAATTTAAATCACCTGTTAT+TGG - Chr2:49732424-49732443 None:intergenic 20.0%
!! TGAATATATAAATGAAAGAG+AGG - Chr2:49730254-49730273 None:intergenic 20.0%
!! TTGTTTCACAAATTAAAAAC+AGG - Chr2:49731957-49731976 None:intergenic 20.0%
!!! ACAACAATCTAAAATAATTC+TGG - Chr2:49734112-49734131 None:intergenic 20.0%
!!! ATCAAATATCTTGTTTTCAA+CGG - Chr2:49733387-49733406 None:intergenic 20.0%
!!! ATTTTTTTGCTTGATGATTT+TGG + Chr2:49732260-49732279 MsG0280009299.01.T01:intron 20.0%
!!! GTTATGGTAATTTGATTTTA+GGG + Chr2:49731612-49731631 MsG0280009299.01.T01:intron 20.0%
!!! TAATAGATGATAATGTTAGA+AGG + Chr2:49733035-49733054 MsG0280009299.01.T01:intron 20.0%
!!! TTTTAGATTGTTGTTCTATT+TGG + Chr2:49734118-49734137 MsG0280009299.01.T01:three_prime_UTR 20.0%
! AAATCAAAACAGAAGTGATA+AGG - Chr2:49731874-49731893 None:intergenic 25.0%
! AAGAAAACCAATAATATCGT+TGG + Chr2:49731561-49731580 MsG0280009299.01.T01:intron 25.0%
! ATCATCATCAAAACTGTTAA+TGG + Chr2:49730522-49730541 MsG0280009299.01.T01:intron 25.0%
! ATCTATTCCCTTAATTAATG+AGG + Chr2:49732163-49732182 MsG0280009299.01.T01:intron 25.0%
! ATGTAAATATTACTGTTTGC+AGG + Chr2:49731123-49731142 MsG0280009299.01.T01:intron 25.0%
! CATATATGTTGAACTTTATG+TGG + Chr2:49731021-49731040 MsG0280009299.01.T01:intron 25.0%
! CTAGCAGTGAAAAATAAATA+TGG + Chr2:49730431-49730450 MsG0280009299.01.T01:intron 25.0%
! GACATCAATGTATCAATTAA+CGG - Chr2:49734067-49734086 None:intergenic 25.0%
! GATAAATAACACAAATCTGT+CGG - Chr2:49733999-49734018 None:intergenic 25.0%
! GTACAAATTACAATATGTCA+TGG - Chr2:49734213-49734232 None:intergenic 25.0%
! GTATTACTTACTTTAGACAA+TGG + Chr2:49732967-49732986 MsG0280009299.01.T01:intron 25.0%
! GTATTCACTCTTAATATCAT+TGG - Chr2:49731515-49731534 None:intergenic 25.0%
! TATGCAGAAAATTCATTTGA+AGG - Chr2:49731005-49731024 None:intergenic 25.0%
! TATTCACCAGATTAATATGT+GGG + Chr2:49732106-49732125 MsG0280009299.01.T01:intron 25.0%
! TGATATTAAGAGTGAATACT+TGG + Chr2:49731516-49731535 MsG0280009299.01.T01:intron 25.0%
! TGTGAAACAATTCATAGATT+AGG + Chr2:49731967-49731986 MsG0280009299.01.T01:intron 25.0%
! TGTGAAATTATTGAATAGAC+TGG + Chr2:49734281-49734300 MsG0280009299.01.T01:three_prime_UTR 25.0%
! TTCTAAACCTGATTAAATGA+TGG - Chr2:49731086-49731105 None:intergenic 25.0%
! TTGTAGATAACAGTAACAAA+TGG - Chr2:49730225-49730244 None:intergenic 25.0%
! TTTATGCAGTTATCTATTCA+AGG + Chr2:49731167-49731186 MsG0280009299.01.T01:intron 25.0%
! TTTGAGGGAAATAATATTGT+TGG + Chr2:49731590-49731609 MsG0280009299.01.T01:intron 25.0%
!! GGAAATAATATTGTTGGTTA+TGG + Chr2:49731596-49731615 MsG0280009299.01.T01:intron 25.0%
!! TACGTCAAAAAAGTATAATG+TGG - Chr2:49733490-49733509 None:intergenic 25.0%
!! TCTTTTGTATCTTCATTACT+TGG + Chr2:49730360-49730379 MsG0280009299.01.T01:intron 25.0%
!! TTTTACGTGTCTTCAAATTT+AGG + Chr2:49731703-49731722 MsG0280009299.01.T01:intron 25.0%
!! TTTTTGCTTAGAATTAAGTC+TGG - Chr2:49733432-49733451 None:intergenic 25.0%
!!! AAATGCAAATTGTGTTGTTT+TGG + Chr2:49731353-49731372 MsG0280009299.01.T01:intron 25.0%
!!! ATATCGTTGGTTATTTTTTG+AGG + Chr2:49731574-49731593 MsG0280009299.01.T01:intron 25.0%
!!! CATTTTGATTAAAAGCTGAA+GGG - Chr2:49732381-49732400 None:intergenic 25.0%
!!! GATCTATATTTTTGTTCTTC+CGG + Chr2:49730867-49730886 MsG0280009299.01.T01:intron 25.0%
!!! GGTTATGGTAATTTGATTTT+AGG + Chr2:49731611-49731630 MsG0280009299.01.T01:intron 25.0%
!!! TATCGTTGGTTATTTTTTGA+GGG + Chr2:49731575-49731594 MsG0280009299.01.T01:intron 25.0%
!!! TCATTTTGATTAAAAGCTGA+AGG - Chr2:49732382-49732401 None:intergenic 25.0%
!!! TGATTCCATTTTTGTTCTAT+AGG + Chr2:49729750-49729769 MsG0280009299.01.T01:intron 25.0%
AATAAATTCAGAATGCCACA+TGG - Chr2:49733744-49733763 None:intergenic 30.0%
ACTATGTATAAACCCAAATG+TGG + Chr2:49731925-49731944 MsG0280009299.01.T01:intron 30.0%
AGAAGCCTATAGAACAAAAA+TGG - Chr2:49729758-49729777 None:intergenic 30.0%
AGTTAACGTAACATAATAGC+TGG - Chr2:49730072-49730091 None:intergenic 30.0%
ATAGAACCACTCTGATTAAT+AGG - Chr2:49732785-49732804 None:intergenic 30.0%
ATATAGAGAGCCTAATCAAT+TGG - Chr2:49732291-49732310 None:intergenic 30.0%
ATTAAGCAATGTACCTTACT+TGG - Chr2:49730191-49730210 None:intergenic 30.0%
ATTCACCAGATTAATATGTG+GGG + Chr2:49732107-49732126 MsG0280009299.01.T01:intron 30.0%
CAAGAAAGAAAGAATATACC+AGG + Chr2:49732750-49732769 MsG0280009299.01.T01:intron 30.0%
CATTACTTGGACTATGAATA+GGG + Chr2:49730373-49730392 MsG0280009299.01.T01:intron 30.0%
CTCTATACCATCATTTAATC+AGG + Chr2:49731076-49731095 MsG0280009299.01.T01:intron 30.0%
GTATTCACCAGATTAATATG+TGG + Chr2:49732105-49732124 MsG0280009299.01.T01:intron 30.0%
GTCCTAGAAGAAAATTGTAT+TGG - Chr2:49731247-49731266 None:intergenic 30.0%
TAACAGCAAGAATTAGACAT+TGG - Chr2:49730756-49730775 None:intergenic 30.0%
TAAGTGCTTAACTGCATATT+TGG + Chr2:49732457-49732476 MsG0280009299.01.T01:intron 30.0%
TACAAAAATCACCACCATAA+GGG - Chr2:49733017-49733036 None:intergenic 30.0%
TAGAACCACTCTGATTAATA+GGG - Chr2:49732784-49732803 None:intergenic 30.0%
TAGGGTTCAAAAATAAGAGT+TGG + Chr2:49731630-49731649 MsG0280009299.01.T01:intron 30.0%
TATTCCCTTAATTAATGAGG+CGG + Chr2:49732166-49732185 MsG0280009299.01.T01:intron 30.0%
TCATTACTTGGACTATGAAT+AGG + Chr2:49730372-49730391 MsG0280009299.01.T01:intron 30.0%
TCTAGCATTATGCATTGTTT+GGG + Chr2:49730908-49730927 MsG0280009299.01.T01:intron 30.0%
TTACAAAAATCACCACCATA+AGG - Chr2:49733018-49733037 None:intergenic 30.0%
TTGATTATTGTGTCTTTGTC+TGG + Chr2:49730632-49730651 MsG0280009299.01.T01:intron 30.0%
TTTATAACATAAGCTGCAGT+TGG + Chr2:49732696-49732715 MsG0280009299.01.T01:intron 30.0%
TTTCAGATTGAACACATGTT+TGG - Chr2:49733691-49733710 None:intergenic 30.0%
TTTGGATGTACCAATTGATT+AGG + Chr2:49732278-49732297 MsG0280009299.01.T01:intron 30.0%
TTTGTACATAATGACTGATG+TGG + Chr2:49734226-49734245 MsG0280009299.01.T01:three_prime_UTR 30.0%
TTTGTGAAAAGAATTGTCCA+TGG + Chr2:49732002-49732021 MsG0280009299.01.T01:intron 30.0%
! AAATCTTCTTTTTCCCCTTA+TGG + Chr2:49733000-49733019 MsG0280009299.01.T01:intron 30.0%
! AGCCAATACAATTTTCTTCT+AGG + Chr2:49731242-49731261 MsG0280009299.01.T01:intron 30.0%
! AGTTGAGAAATGTACTCTTT+TGG + Chr2:49732809-49732828 MsG0280009299.01.T01:intron 30.0%
! ATGTGTTCAATCTGAAATGT+TGG + Chr2:49733694-49733713 MsG0280009299.01.T01:intron 30.0%
! ATTTTCTTGAAGCACAAAAC+TGG - Chr2:49730943-49730962 None:intergenic 30.0%
! ATTTTGTCTATATTGAGTCC+AGG + Chr2:49733630-49733649 MsG0280009299.01.T01:intron 30.0%
! TATTGTTGTAGCACTTTTGA+TGG + Chr2:49729717-49729736 MsG0280009299.01.T01:intron 30.0%
! TTTTCTTGAAGCACAAAACT+GGG - Chr2:49730942-49730961 None:intergenic 30.0%
! TTTTGTCTATATTGAGTCCA+GGG + Chr2:49733631-49733650 MsG0280009299.01.T01:intron 30.0%
! TTTTTCCTTAATACCCAACT+TGG - Chr2:49732931-49732950 None:intergenic 30.0%
!! AAGTCTCTGAAATGTAAGAT+TGG + Chr2:49730685-49730704 MsG0280009299.01.T01:intron 30.0%
!! ACAATGAAATTGGTAGTGAA+TGG + Chr2:49731265-49731284 MsG0280009299.01.T01:intron 30.0%
!! AGAGTTTAAGTTGCTCTAAA+TGG - Chr2:49732231-49732250 None:intergenic 30.0%
!! AGTTTAAGTTGCTCTAAATG+GGG - Chr2:49732229-49732248 None:intergenic 30.0%
!! ATATTTTTGTTCTTCCGGAA+AGG + Chr2:49730872-49730891 MsG0280009299.01.T01:intron 30.0%
!! GAGTTTAAGTTGCTCTAAAT+GGG - Chr2:49732230-49732249 None:intergenic 30.0%
!! TTCTTCTAGGACAATGAAAT+TGG + Chr2:49731255-49731274 MsG0280009299.01.T01:intron 30.0%
!!! ATTGTGTTGTTTTGGTCATT+TGG + Chr2:49731361-49731380 MsG0280009299.01.T01:intron 30.0%
!!! TGTTCTATTTGGTTTTGCAA+CGG + Chr2:49734129-49734148 MsG0280009299.01.T01:three_prime_UTR 30.0%
!!! TTGGTTCTGTTTCTTTTTAC+AGG + Chr2:49733213-49733232 MsG0280009299.01.T01:intron 30.0%
AACATTAAACAGGGTGAAGT+TGG + Chr2:49733603-49733622 MsG0280009299.01.T01:intron 35.0%
AAGAAACATCTGCATTGTGT+AGG - Chr2:49732678-49732697 None:intergenic 35.0%
AAGTCCGCCTCATTAATTAA+GGG - Chr2:49732173-49732192 None:intergenic 35.0%
AATTGTGCAATCAAAGAGCA+TGG - Chr2:49729922-49729941 None:intergenic 35.0%
ACAAAAATCACCACCATAAG+GGG - Chr2:49733016-49733035 None:intergenic 35.0%
ATAGTAGATGCGTTAAGAAC+AGG + Chr2:49733077-49733096 MsG0280009299.01.T01:CDS 35.0%
ATTAAAAGCTGAAGGGATAG+CGG - Chr2:49732374-49732393 None:intergenic 35.0%
CAAAGAGCTCTTTACTCTTT+AGG - Chr2:49731762-49731781 None:intergenic 35.0%
CTCAACACAATTAATCAGAG+TGG - Chr2:49732318-49732337 None:intergenic 35.0%
CTCTAGCATTATGCATTGTT+TGG + Chr2:49730907-49730926 MsG0280009299.01.T01:intron 35.0%
GCATAAAAGTTCCCAATAAC+AGG + Chr2:49732409-49732428 MsG0280009299.01.T01:intron 35.0%
GTACAATTCACATTCTTCGA+AGG - Chr2:49732585-49732604 None:intergenic 35.0%
GTCTAAACAATCTGAACCAA+AGG + Chr2:49729661-49729680 MsG0280009299.01.T01:CDS 35.0%
TAACAAAACCAGCGAAATTC+TGG - Chr2:49734170-49734189 None:intergenic 35.0%
TAAGGTGGTATAGGTACAAA+GGG + Chr2:49729591-49729610 MsG0280009299.01.T01:CDS 35.0%
TAAGTCTGGAGAGAATCAAA+AGG - Chr2:49733418-49733437 None:intergenic 35.0%
TGTTCAACATCTTGATATGC+AGG - Chr2:49732552-49732571 None:intergenic 35.0%
TTAAGGTGGTATAGGTACAA+AGG + Chr2:49729590-49729609 MsG0280009299.01.T01:CDS 35.0%
TTAGTGGTGTTTAGTGGTTA+AGG + Chr2:49729684-49729703 MsG0280009299.01.T01:intron 35.0%
TTCATATATTAGCACTACCC+TGG - Chr2:49733651-49733670 None:intergenic 35.0%
! TTCTGAAGATGCTAAAAGGT+TGG + Chr2:49729774-49729793 MsG0280009299.01.T01:CDS 35.0%
! TTGTGTGTTTTCTTACTGTC+AGG + Chr2:49730106-49730125 MsG0280009299.01.T01:intron 35.0%
!! TCTAATTCTTGCTGTTACTG+AGG + Chr2:49730759-49730778 MsG0280009299.01.T01:intron 35.0%
!! TCTCTGAAATGTAAGATTGG+AGG + Chr2:49730688-49730707 MsG0280009299.01.T01:intron 35.0%
AAATAAGCAGCTGCACCAAT+TGG - Chr2:49733323-49733342 None:intergenic 40.0%
AACAGGAGCAACTACTATGA+AGG + Chr2:49733094-49733113 MsG0280009299.01.T01:CDS 40.0%
AATGTAAGATTGGAGGTGAG+TGG + Chr2:49730695-49730714 MsG0280009299.01.T01:intron 40.0%
ACTGCATAAAAGCAGATAGC+AGG - Chr2:49731157-49731176 None:intergenic 40.0%
AGAAATCTGCTTCTTCCTTC+TGG + Chr2:49734249-49734268 MsG0280009299.01.T01:three_prime_UTR 40.0%
AGAATTGGAGAATGCTGAGT+TGG + Chr2:49733798-49733817 MsG0280009299.01.T01:CDS 40.0%
AGAGCAAGACAAAACCTTTC+CGG - Chr2:49730889-49730908 None:intergenic 40.0%
AGATACTATCCATCATCGCA+TGG - Chr2:49730826-49730845 None:intergenic 40.0%
AGGTACAAAGGGAATGATGA+TGG + Chr2:49729602-49729621 MsG0280009299.01.T01:CDS 40.0%
ATAGGGCAATGGAACAAAAC+AGG + Chr2:49732202-49732221 MsG0280009299.01.T01:intron 40.0%
ATGGAAGACACTTATCGTCA+CGG - Chr2:49734194-49734213 None:intergenic 40.0%
ATGTAAGATTGGAGGTGAGT+GGG + Chr2:49730696-49730715 MsG0280009299.01.T01:intron 40.0%
CAAAGGTTAGTGGTGTTTAG+TGG + Chr2:49729678-49729697 MsG0280009299.01.T01:intron 40.0%
CAACTCGATCGTTAAAGACT+TGG - Chr2:49733161-49733180 None:intergenic 40.0%
CAAGTCCGCCTCATTAATTA+AGG - Chr2:49732174-49732193 None:intergenic 40.0%
CAATCTGAACCAAAGGTTAG+TGG + Chr2:49729668-49729687 MsG0280009299.01.T01:intron 40.0%
CACTAAACACCACTAACCTT+TGG - Chr2:49729680-49729699 None:intergenic 40.0%
CAGATTAATATGTGGGGAGA+AGG + Chr2:49732113-49732132 MsG0280009299.01.T01:intron 40.0%
CGGCAAAATGAATGGTTGAT+TGG - Chr2:49732354-49732373 None:intergenic 40.0%
CTGCATTGTGTAGGAGAATT+TGG - Chr2:49732669-49732688 None:intergenic 40.0%
CTGTTCATTTATCTCGTCCA+TGG - Chr2:49733261-49733280 None:intergenic 40.0%
CTTCTCCCCACATATTAATC+TGG - Chr2:49732115-49732134 None:intergenic 40.0%
GAATACACTCATCCTTCTTG+AGG - Chr2:49732091-49732110 None:intergenic 40.0%
GAATGGATCGTCTATAACTC+AGG + Chr2:49731282-49731301 MsG0280009299.01.T01:intron 40.0%
GGAAGCAGAACTTGAAGAAT+TGG + Chr2:49733783-49733802 MsG0280009299.01.T01:CDS 40.0%
GGAGAAGGCTTCAAAAGTAA+CGG + Chr2:49732128-49732147 MsG0280009299.01.T01:intron 40.0%
GTGATCTTAGTTAGCTGATG+AGG - Chr2:49730607-49730626 None:intergenic 40.0%
TAACGCATCTACTATTTCCG+TGG - Chr2:49733072-49733091 None:intergenic 40.0%
TACACAAGCTTCTAAACCAC+AGG + Chr2:49729891-49729910 MsG0280009299.01.T01:CDS 40.0%
TTCACACATACAAAGCCAGA+AGG - Chr2:49734267-49734286 None:intergenic 40.0%
TTGAAGGAGAAATAGGGCAA+TGG + Chr2:49732191-49732210 MsG0280009299.01.T01:intron 40.0%
! AAAGAGCATGGTAAAACCTG+TGG - Chr2:49729910-49729929 None:intergenic 40.0%
! AATTAATGAGGCGGACTTGA+AGG + Chr2:49732175-49732194 MsG0280009299.01.T01:intron 40.0%
! AGGCTTCTGAAGATGCTAAA+AGG + Chr2:49729770-49729789 MsG0280009299.01.T01:intron 40.0%
! GTCCATGGCTGATTTAATTG+AGG + Chr2:49732017-49732036 MsG0280009299.01.T01:intron 40.0%
! GTTTTCTTACTGTCAGGATG+TGG + Chr2:49730112-49730131 MsG0280009299.01.T01:intron 40.0%
! TAGAGGATGCAATGGTGTTT+TGG - Chr2:49731215-49731234 None:intergenic 40.0%
! TCTTCTTTTTCCCCTTATGG+TGG + Chr2:49733003-49733022 MsG0280009299.01.T01:intron 40.0%
! TTGGCAGCAGAAAGTACATA+AGG - Chr2:49732912-49732931 None:intergenic 40.0%
! TTGTTCTTTTCAGCACGAAC+TGG + Chr2:49733762-49733781 MsG0280009299.01.T01:intron 40.0%
! TTTTAACAGAAGCCTGACCA+TGG - Chr2:49730577-49730596 None:intergenic 40.0%
!! CTGAAATGTTGGTGCTACAT+TGG + Chr2:49733705-49733724 MsG0280009299.01.T01:intron 40.0%
!! GAGTGAATACTTGGTGTTTG+AGG + Chr2:49731525-49731544 MsG0280009299.01.T01:intron 40.0%
!! GATGGTACTGATCAAGTTAG+TGG + Chr2:49729620-49729639 MsG0280009299.01.T01:CDS 40.0%
!! GCAGTTGCAGTTTGATTTGA+AGG + Chr2:49730963-49730982 MsG0280009299.01.T01:intron 40.0%
!! GGTTTTAATGCATGTCCATG+TGG + Chr2:49733726-49733745 MsG0280009299.01.T01:intron 40.0%
AAACAGGCAGCTTCCACATT+TGG - Chr2:49731941-49731960 None:intergenic 45.0%
AACAGGCAGCTTCCACATTT+GGG - Chr2:49731940-49731959 None:intergenic 45.0%
AATTCATCTTCCTCAGGCGT+CGG - Chr2:49733916-49733935 None:intergenic 45.0%
ACACCATTGCATCCTCTATC+TGG + Chr2:49731217-49731236 MsG0280009299.01.T01:intron 45.0%
AGCTTAAGCCAGAATTTCGC+TGG + Chr2:49734159-49734178 MsG0280009299.01.T01:three_prime_UTR 45.0%
ATTAATAGGGCAGTCTCACC+TGG - Chr2:49732771-49732790 None:intergenic 45.0%
ATTGCTGCATCAGCCAAGTA+AGG + Chr2:49730175-49730194 MsG0280009299.01.T01:CDS 45.0%
CACCATTGCATCCTCTATCT+GGG + Chr2:49731218-49731237 MsG0280009299.01.T01:intron 45.0%
CGCCTCAATTAAATCAGCCA+TGG - Chr2:49732022-49732041 None:intergenic 45.0%
GACTGCCCTATTAATCAGAG+TGG + Chr2:49732776-49732795 MsG0280009299.01.T01:intron 45.0%
GCGGACTTGAAGGAGAAATA+GGG + Chr2:49732185-49732204 MsG0280009299.01.T01:intron 45.0%
GTACTTTCTGCTGCCAAGTT+GGG + Chr2:49732915-49732934 MsG0280009299.01.T01:intron 45.0%
TACCCAGATAGAGGATGCAA+TGG - Chr2:49731223-49731242 None:intergenic 45.0%
TCTTACTGTCAGGATGTGGA+TGG + Chr2:49730116-49730135 MsG0280009299.01.T01:intron 45.0%
TCTTGAGGAAACAAACTGCG+CGG - Chr2:49732076-49732095 None:intergenic 45.0%
TGCTCTAGCACTCTCGATTT+CGG - Chr2:49729827-49729846 None:intergenic 45.0%
TGGCAAGCTTAAGGTGGTAT+AGG + Chr2:49729582-49729601 MsG0280009299.01.T01:CDS 45.0%
TGTAAGATTGGAGGTGAGTG+GGG + Chr2:49730697-49730716 MsG0280009299.01.T01:intron 45.0%
TGTACTTTCTGCTGCCAAGT+TGG + Chr2:49732914-49732933 MsG0280009299.01.T01:intron 45.0%
! AAATCTGTCGGTTGTGTCAC+GGG - Chr2:49733987-49734006 None:intergenic 45.0%
! AGGGATAGCGGCAAAATGAA+TGG - Chr2:49732362-49732381 None:intergenic 45.0%
! CAAATCTGTCGGTTGTGTCA+CGG - Chr2:49733988-49734007 None:intergenic 45.0%
! CGCAGTTTGTTTCCTCAAGA+AGG + Chr2:49732076-49732095 MsG0280009299.01.T01:intron 45.0%
! TGATGATGTCGACAAGACCA+TGG + Chr2:49733241-49733260 MsG0280009299.01.T01:CDS 45.0%
! TGCTGCCAAGTTGGGTATTA+AGG + Chr2:49732923-49732942 MsG0280009299.01.T01:intron 45.0%
! TGGCTGATTTAATTGAGGCG+TGG + Chr2:49732022-49732041 MsG0280009299.01.T01:intron 45.0%
!! GAAGCATTGTCAGCTCCAAT+TGG + Chr2:49733305-49733324 MsG0280009299.01.T01:CDS 45.0%
!! GGATGCAATGGTGTTTTGGA+AGG - Chr2:49731211-49731230 None:intergenic 45.0%
!! TCTTGCTGTTACTGAGGAGA+GGG + Chr2:49730765-49730784 MsG0280009299.01.T01:intron 45.0%
!! TGTATTGGCTACCCAGATAG+AGG - Chr2:49731232-49731251 None:intergenic 45.0%
!! TTCTTGCTGTTACTGAGGAG+AGG + Chr2:49730764-49730783 MsG0280009299.01.T01:intron 45.0%
!!! TGCAGCTGCTTATTTTGACG+AGG + Chr2:49733328-49733347 MsG0280009299.01.T01:CDS 45.0%
ACTGGTGGAAGAGGTTCAAG+AGG + Chr2:49730139-49730158 MsG0280009299.01.T01:CDS 50.0%
AGATGAATTGGCAGCTCTGC+AGG + Chr2:49733927-49733946 MsG0280009299.01.T01:CDS 50.0%
CGCATCTACTATTTCCGTGG+TGG - Chr2:49733069-49733088 None:intergenic 50.0%
GAATGCTTTGCAAAGCACGC+AGG + Chr2:49730719-49730738 MsG0280009299.01.T01:intron 50.0%
GACGCCTGAGGAAGATGAAT+TGG + Chr2:49733915-49733934 MsG0280009299.01.T01:CDS 50.0%
GCTGCCAATTCATCTTCCTC+AGG - Chr2:49733922-49733941 None:intergenic 50.0%
GGAGCAGGAGCTGTAAATGT+TGG - Chr2:49733841-49733860 None:intergenic 50.0%
GTTCGTTGACCATGCGATGA+TGG + Chr2:49730814-49730833 MsG0280009299.01.T01:intron 50.0%
! GGCGGACTTGAAGGAGAAAT+AGG + Chr2:49732184-49732203 MsG0280009299.01.T01:intron 50.0%
!! AATCGAGAGTGCTAGAGCAG+TGG + Chr2:49729828-49729847 MsG0280009299.01.T01:CDS 50.0%
!! AGAAGGTGCTAAAGCCACCA+CGG + Chr2:49733052-49733071 MsG0280009299.01.T01:CDS 50.0%
AACAGAAGCCTGACCATGGC+AGG - Chr2:49730573-49730592 None:intergenic 55.0%
AGTGCTAGAGCAGTGGTGCT+GGG + Chr2:49729835-49729854 MsG0280009299.01.T01:CDS 55.0%
CCACAGTGCATGTCTCAGCT+GGG + Chr2:49733860-49733879 MsG0280009299.01.T01:CDS 55.0%
CCAGCTGAGACATGCACTGT+GGG - Chr2:49733862-49733881 None:intergenic 55.0%
CTGTCAGGATGTGGATGGAC+TGG + Chr2:49730121-49730140 MsG0280009299.01.T01:intron 55.0%
GAGGAAACAAACTGCGCGGA+CGG - Chr2:49732072-49732091 None:intergenic 55.0%
GCTGAGATGGCACTTTGAGC+GGG + Chr2:49733949-49733968 MsG0280009299.01.T01:exon 55.0%
GGCTGAGATGGCACTTTGAG+CGG + Chr2:49733948-49733967 MsG0280009299.01.T01:exon 55.0%
TTTCAACGCAGCTCCTGCCA+TGG + Chr2:49730557-49730576 MsG0280009299.01.T01:intron 55.0%
TTTGCAGGAACAGGACGTGC+AGG - Chr2:49733892-49733911 None:intergenic 55.0%
! AGAGCAGTGGTGCTGGGAAT+TGG + Chr2:49729841-49729860 MsG0280009299.01.T01:CDS 55.0%
! AGCAGTGGTGCTGGGAATTG+GGG + Chr2:49729843-49729862 MsG0280009299.01.T01:CDS 55.0%
! GAGCAGTGGTGCTGGGAATT+GGG + Chr2:49729842-49729861 MsG0280009299.01.T01:CDS 55.0%
!! TCAGGATGTGGATGGACTGG+TGG + Chr2:49730124-49730143 MsG0280009299.01.T01:intron 55.0%
!! TGTGGATGGACTGGTGGAAG+AGG + Chr2:49730130-49730149 MsG0280009299.01.T01:CDS 55.0%
CAGTGCATGTCTCAGCTGGG+AGG + Chr2:49733863-49733882 MsG0280009299.01.T01:CDS 60.0%
CCCACAGTGCATGTCTCAGC+TGG + Chr2:49733859-49733878 MsG0280009299.01.T01:CDS 60.0%
CCCAGCTGAGACATGCACTG+TGG - Chr2:49733863-49733882 None:intergenic 60.0%
GAGACATGCACTGTGGGAGC+AGG - Chr2:49733856-49733875 None:intergenic 60.0%
GAGTGCTAGAGCAGTGGTGC+TGG + Chr2:49729834-49729853 MsG0280009299.01.T01:CDS 60.0%
! AGTGGTGCTGGGAATTGGGG+AGG + Chr2:49729846-49729865 MsG0280009299.01.T01:CDS 60.0%
ACGCAGCTCCTGCCATGGTC+AGG + Chr2:49730562-49730581 MsG0280009299.01.T01:intron 65.0%
GGCAGCTCTGCAGGCTGAGA+TGG + Chr2:49733936-49733955 MsG0280009299.01.T01:CDS 65.0%
TCCTGCAAAGCCGACGCCTG+AGG + Chr2:49733903-49733922 MsG0280009299.01.T01:CDS 65.0%
!! GGCGTCGGCTTTGCAGGAAC+AGG - Chr2:49733901-49733920 None:intergenic 65.0%
!! TCCTCAGGCGTCGGCTTTGC+AGG - Chr2:49733907-49733926 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 49729581 49734351 49729581 ID=MsG0280009299.01;Name=MsG0280009299.01
Chr2 mRNA 49729581 49734351 49729581 ID=MsG0280009299.01.T01;Parent=MsG0280009299.01;Name=MsG0280009299.01.T01;_AED=0.44;_eAED=0.45;_QI=0|0.8|0.83|1|0.8|0.83|6|385|236
Chr2 exon 49729581 49729682 49729581 ID=MsG0280009299.01.T01:exon:45205;Parent=MsG0280009299.01.T01
Chr2 exon 49729772 49729912 49729772 ID=MsG0280009299.01.T01:exon:45206;Parent=MsG0280009299.01.T01
Chr2 exon 49730128 49730196 49730128 ID=MsG0280009299.01.T01:exon:45207;Parent=MsG0280009299.01.T01
Chr2 exon 49733041 49733132 49733041 ID=MsG0280009299.01.T01:exon:45208;Parent=MsG0280009299.01.T01
Chr2 exon 49733235 49733349 49733235 ID=MsG0280009299.01.T01:exon:45209;Parent=MsG0280009299.01.T01
Chr2 exon 49733775 49734351 49733775 ID=MsG0280009299.01.T01:exon:45210;Parent=MsG0280009299.01.T01
Chr2 CDS 49729581 49729682 49729581 ID=MsG0280009299.01.T01:cds;Parent=MsG0280009299.01.T01
Chr2 CDS 49729772 49729912 49729772 ID=MsG0280009299.01.T01:cds;Parent=MsG0280009299.01.T01
Chr2 CDS 49730128 49730196 49730128 ID=MsG0280009299.01.T01:cds;Parent=MsG0280009299.01.T01
Chr2 CDS 49733041 49733132 49733041 ID=MsG0280009299.01.T01:cds;Parent=MsG0280009299.01.T01
Chr2 CDS 49733235 49733349 49733235 ID=MsG0280009299.01.T01:cds;Parent=MsG0280009299.01.T01
Chr2 CDS 49733775 49733966 49733775 ID=MsG0280009299.01.T01:cds;Parent=MsG0280009299.01.T01
Chr2 three_prime_UTR 49733967 49734351 49733967 ID=MsG0280009299.01.T01:three_prime_utr;Parent=MsG0280009299.01.T01
Gene Sequence

>MsG0280009299.01.T01

ATGGCAAGCTTAAGGTGGTATAGGTACAAAGGGAATGATGATGGTACTGATCAAGTTAGTGGTGTTGAACTTGATAAAGTGTCTAAACAATCTGAACCAAAGGCTTCTGAAGATGCTAAAAGGTTGGTTAATCAAGAGAAGTCTTTTGCTTGTGCCGAAATCGAGAGTGCTAGAGCAGTGGTGCTGGGAATTGGGGAGGCACTTGAAGAACAAGAAAAAGTTACACAAGCTTCTAAACCACAGGATGTGGATGGACTGGTGGAAGAGGTTCAAGAGGCTAGAAGAATCAGATTGCTGCATCAGCCAAGTAAGATGATAATGTTAGAAGGTGCTAAAGCCACCACGGAAATAGTAGATGCGTTAAGAACAGGAGCAACTACTATGAAGGCTATGCAGAAAGCAACGAATATTGATGATGTCGACAAGACCATGGACGAGATAAATGAACAGACTGAGAATATGAAGCAGATTCAAGAAGCATTGTCAGCTCCAATTGGTGCAGCTGCTTATTTTGACGAGCACGAACTGGAAGCAGAACTTGAAGAATTGGAGAATGCTGAGTTGGAAGAACAACTTCTTCAGCCAACATTTACAGCTCCTGCTCCCACAGTGCATGTCTCAGCTGGGAGGCAACCTGCACGTCCTGTTCCTGCAAAGCCGACGCCTGAGGAAGATGAATTGGCAGCTCTGCAGGCTGAGATGGCACTTTGA
Protein sequence

>MsG0280009299.01.T01

MASLRWYRYKGNDDGTDQVSGVELDKVSKQSEPKASEDAKRLVNQEKSFACAEIESARAVVLGIGEALEEQEKVTQASKPQDVDGLVEEVQEARRIRLLHQPSKMIMLEGAKATTEIVDALRTGATTMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALSAPIGAAAYFDEHELEAELEELENAELEEQLLQPTFTAPAPTVHVSAGRQPARPVPAKPTPEEDELAALQAEMAL*