AlfalfaGEDB | MsG0580026726.01

M. sativa cultivar ZhongmuNo.1 / MsG0580026726.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026726.01.T01	XP_039683451.1	91.538	130	11	0	1	130	1	130	4.39E-77	234

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026726.01.T01	A0A072TGZ8	90.769	130	12	0	1	130	1	130	1.35e-76	232

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047957.01	MsG0580026726.01	0.802374	5.781743e-49	2.827755e-46
MsG0080047959.01	MsG0580026726.01	0.804163	2.464495e-49	1.261748e-46
MsG0080048404.01	MsG0580026726.01	0.811788	5.875442e-51	3.670670e-48
MsG0080048462.01	MsG0580026726.01	0.808063	3.723291e-50	2.107949e-47
MsG0080048618.01	MsG0580026726.01	0.835937	1.254523e-56	1.557174e-53
MsG0080048683.01	MsG0580026726.01	0.806868	6.672805e-50	3.662103e-47
MsG0080048856.01	MsG0580026726.01	0.812748	3.627653e-51	2.325438e-48
MsG0080048874.01	MsG0580026726.01	0.812149	4.902815e-51	3.093140e-48
MsG0180000065.01	MsG0580026726.01	0.809251	2.075701e-50	1.212668e-47
MsG0180000091.01	MsG0580026726.01	0.821764	3.393883e-53	2.786611e-50
MsG0180000145.01	MsG0580026726.01	0.810032	1.410419e-50	8.412173e-48
MsG0180000335.01	MsG0580026726.01	0.819962	8.817462e-53	6.885486e-50
MsG0180000470.01	MsG0580026726.01	0.862817	3.872816e-64	1.149544e-60
MsG0180000550.01	MsG0580026726.01	0.805208	1.491543e-49	7.844597e-47
MsG0180000604.01	MsG0580026726.01	0.802691	4.976020e-49	2.453226e-46
MsG0180000703.01	MsG0580026726.01	0.828708	7.736653e-55	7.750420e-52
MsG0180000914.01	MsG0580026726.01	0.807930	3.972749e-50	2.241246e-47
MsG0180001063.01	MsG0580026726.01	0.869100	3.986258e-66	1.477603e-62
MsG0180002377.01	MsG0580026726.01	0.808532	2.957698e-50	1.695282e-47
MsG0180002842.01	MsG0580026726.01	0.834349	3.155390e-56	3.734653e-53
MsG0180002952.01	MsG0580026726.01	0.808326	3.272949e-50	1.865983e-47
MsG0180003669.01	MsG0580026726.01	0.827141	1.842714e-54	1.763611e-51
MsG0180003673.01	MsG0580026726.01	0.806010	1.012431e-49	5.435730e-47
MsG0180003686.01	MsG0580026726.01	0.815323	9.806982e-52	6.740003e-49
MsG0180003797.01	MsG0580026726.01	0.802107	6.561633e-49	3.187229e-46
MsG0180003952.01	MsG0580026726.01	0.802919	4.464468e-49	2.213888e-46
MsG0180004264.01	MsG0580026726.01	0.817945	2.533960e-52	1.871140e-49
MsG0180004295.01	MsG0580026726.01	0.804111	2.527076e-49	1.292075e-46
MsG0180004389.01	MsG0580026726.01	0.807114	5.920641e-50	3.269900e-47
MsG0180004463.01	MsG0580026726.01	0.822661	2.102531e-53	1.771057e-50
MsG0180004465.01	MsG0580026726.01	0.819664	1.031641e-52	7.988774e-50
MsG0180004500.01	MsG0580026726.01	0.805179	1.512754e-49	7.950149e-47
MsG0180004765.01	MsG0580026726.01	0.802916	4.470776e-49	2.216866e-46
MsG0180004789.01	MsG0580026726.01	0.801045	1.083410e-48	5.123329e-46
MsG0180004926.01	MsG0580026726.01	0.827819	1.266975e-54	1.236723e-51
MsG0180004943.01	MsG0580026726.01	0.836232	1.055762e-56	1.322506e-53
MsG0180005050.01	MsG0580026726.01	0.829589	4.731361e-55	4.863845e-52
MsG0180005250.01	MsG0580026726.01	0.819526	1.108980e-52	8.555158e-50
MsG0180005445.01	MsG0580026726.01	0.820041	8.458558e-53	6.620042e-50
MsG0180005763.01	MsG0580026726.01	0.809745	1.625847e-50	9.624530e-48
MsG0180005960.01	MsG0580026726.01	0.807391	5.171899e-50	2.877196e-47
MsG0280006299.01	MsG0580026726.01	0.824716	6.940544e-54	6.198020e-51
MsG0280006375.01	MsG0580026726.01	0.818853	1.578203e-52	1.195014e-49
MsG0280010698.01	MsG0580026726.01	0.832632	8.463572e-56	9.514623e-53
MsG0280010722.01	MsG0580026726.01	0.835494	1.624232e-56	1.989520e-53
MsG0280011052.01	MsG0580026726.01	0.808361	3.216111e-50	1.835308e-47
MsG0280011175.01	MsG0580026726.01	0.827255	1.730498e-54	1.661829e-51
MsG0380011494.01	MsG0580026726.01	0.816391	5.665173e-52	4.009102e-49
MsG0380011581.01	MsG0580026726.01	0.805629	1.216995e-49	6.470330e-47
MsG0380011609.01	MsG0580026726.01	0.833912	4.060944e-56	4.744283e-53
MsG0380011755.01	MsG0580026726.01	0.801282	9.689804e-49	4.609432e-46
MsG0380012067.01	MsG0580026726.01	0.811615	6.406004e-51	3.983807e-48
MsG0380012330.01	MsG0580026726.01	0.835464	1.652421e-56	2.022238e-53
MsG0380012361.01	MsG0580026726.01	0.801524	8.645595e-49	4.137838e-46
MsG0380012403.01	MsG0580026726.01	0.810557	1.086156e-50	6.567980e-48
MsG0380012460.01	MsG0580026726.01	0.851159	1.064229e-60	2.134623e-57
MsG0380013363.01	MsG0580026726.01	0.817418	3.331699e-52	2.425082e-49
MsG0380013475.01	MsG0580026726.01	0.802645	5.084265e-49	2.503644e-46
MsG0380013549.01	MsG0580026726.01	0.811430	7.029104e-51	4.350228e-48
MsG0380013834.01	MsG0580026726.01	0.816612	5.056303e-52	3.599861e-49
MsG0380014468.01	MsG0580026726.01	0.808084	3.685546e-50	2.087731e-47
MsG0380014537.01	MsG0580026726.01	0.824790	6.668894e-54	5.967865e-51
MsG0380014573.01	MsG0580026726.01	0.807066	6.059738e-50	3.342648e-47
MsG0480018465.01	MsG0580026726.01	0.807217	5.629602e-50	3.117668e-47
MsG0480018653.01	MsG0580026726.01	0.814876	1.232482e-51	8.367619e-49
MsG0480019206.01	MsG0580026726.01	0.815520	8.865618e-52	6.126350e-49
MsG0480019297.01	MsG0580026726.01	0.811992	5.305130e-51	3.332569e-48
MsG0480019435.01	MsG0580026726.01	0.800083	1.702370e-48	7.855513e-46
MsG0480019795.01	MsG0580026726.01	0.806663	7.373514e-50	4.025015e-47
MsG0480019970.01	MsG0580026726.01	0.821985	3.017414e-53	2.493053e-50
MsG0480020136.01	MsG0580026726.01	0.803025	4.243830e-49	2.110068e-46
MsG0480020634.01	MsG0580026726.01	0.802730	4.884563e-49	2.410643e-46
MsG0480020739.01	MsG0580026726.01	0.819929	8.969054e-53	6.997522e-50
MsG0480020836.01	MsG0580026726.01	0.819010	1.453457e-52	1.105405e-49
MsG0480020887.01	MsG0580026726.01	0.801769	7.699579e-49	3.707849e-46
MsG0480021274.01	MsG0580026726.01	0.802245	6.148230e-49	2.996963e-46
MsG0480021536.01	MsG0580026726.01	0.827523	1.493092e-54	1.444931e-51
MsG0480021869.01	MsG0580026726.01	0.804592	2.006274e-49	1.038552e-46
MsG0480021870.01	MsG0580026726.01	0.823489	1.347315e-53	1.161791e-50
MsG0480021925.01	MsG0580026726.01	0.818186	2.235091e-52	1.661490e-49
MsG0480022068.01	MsG0580026726.01	0.843423	1.413745e-58	2.211001e-55
MsG0480022222.01	MsG0580026726.01	0.808376	3.192372e-50	1.822451e-47
MsG0480022259.01	MsG0580026726.01	0.840774	7.099356e-58	1.021864e-54
MsG0480022262.01	MsG0580026726.01	0.834378	3.102836e-56	3.675804e-53
MsG0480022551.01	MsG0580026726.01	0.803599	3.227655e-49	1.628768e-46
MsG0480022707.01	MsG0580026726.01	0.802834	4.649048e-49	2.300567e-46
MsG0480022984.01	MsG0580026726.01	0.817814	2.712176e-52	1.995679e-49
MsG0480022985.01	MsG0580026726.01	0.819731	9.956919e-53	7.725117e-50
MsG0480023145.01	MsG0580026726.01	0.801608	8.311500e-49	3.986138e-46
MsG0480023251.01	MsG0580026726.01	0.824086	9.765987e-54	8.563853e-51
MsG0480023463.01	MsG0580026726.01	0.821239	4.486959e-53	3.630311e-50
MsG0480023503.01	MsG0580026726.01	0.810233	1.276174e-50	7.651060e-48
MsG0480023873.01	MsG0580026726.01	0.800722	1.261429e-48	5.915863e-46
MsG0580024038.01	MsG0580026726.01	0.807905	4.021797e-50	2.267372e-47
MsG0580024114.01	MsG0580026726.01	0.806729	7.141615e-50	3.905257e-47
MsG0580024197.01	MsG0580026726.01	0.800678	1.287530e-48	6.031749e-46
MsG0580024472.01	MsG0580026726.01	0.807767	4.303680e-50	2.417654e-47
MsG0580024552.01	MsG0580026726.01	0.831266	1.839853e-55	1.986296e-52
MsG0580024690.01	MsG0580026726.01	0.814230	1.712891e-51	1.142845e-48
MsG0580024781.01	MsG0580026726.01	0.812958	3.262875e-51	2.103078e-48
MsG0580025022.01	MsG0580026726.01	0.821144	4.719221e-53	3.808151e-50
MsG0580025448.01	MsG0580026726.01	0.803539	3.321433e-49	1.673530e-46
MsG0580025758.01	MsG0580026726.01	0.818323	2.080941e-52	1.552725e-49
MsG0580025816.01	MsG0580026726.01	0.811644	6.314818e-51	3.929971e-48
MsG0580025987.01	MsG0580026726.01	0.837250	5.807927e-57	7.500068e-54
MsG0380014948.01	MsG0580026726.01	0.832389	9.720753e-56	1.084951e-52
MsG0380015332.01	MsG0580026726.01	0.818043	2.408422e-52	1.783175e-49
MsG0380015555.01	MsG0580026726.01	0.823736	1.179767e-53	1.024556e-50
MsG0380015566.01	MsG0580026726.01	0.802772	4.786804e-49	2.364968e-46
MsG0380016407.01	MsG0580026726.01	0.812973	3.236966e-51	2.087338e-48
MsG0380016517.01	MsG0580026726.01	0.806396	8.395878e-50	4.552000e-47
MsG0380016910.01	MsG0580026726.01	0.837089	6.383228e-57	8.203032e-54
MsG0380017461.01	MsG0580026726.01	0.823238	1.542670e-53	1.320763e-50
MsG0380017498.01	MsG0580026726.01	0.818706	1.704134e-52	1.285034e-49
MsG0380017680.01	MsG0580026726.01	0.819530	1.106514e-52	8.537308e-50
MsG0380018075.01	MsG0580026726.01	0.818337	2.066593e-52	1.542664e-49
MsG0480018163.01	MsG0580026726.01	0.809043	2.299874e-50	1.336148e-47
MsG0480018183.01	MsG0580026726.01	0.803610	3.210637e-49	1.620595e-46
MsG0480018251.01	MsG0580026726.01	0.812289	4.569038e-51	2.893537e-48
MsG0580026122.01	MsG0580026726.01	0.804531	2.066210e-49	1.067842e-46
MsG0580026131.01	MsG0580026726.01	0.815039	1.133988e-51	7.733398e-49
MsG0580026726.01	MsG0580027833.01	0.818171	2.252804e-52	1.673920e-49
MsG0580026726.01	MsG0580027922.01	0.805967	1.033511e-49	5.542841e-47
MsG0580026726.01	MsG0580027937.01	0.801653	8.136875e-49	3.906848e-46
MsG0580026726.01	MsG0580028479.01	0.811169	8.009399e-51	4.922648e-48
MsG0580026726.01	MsG0580028517.01	0.803160	3.980082e-49	1.985874e-46
MsG0580026726.01	MsG0580028527.01	0.801761	7.729427e-49	3.721419e-46
MsG0580026726.01	MsG0580029008.01	0.835142	1.992836e-56	2.415295e-53
MsG0580026726.01	MsG0580029206.01	0.800211	1.602823e-48	7.420341e-46
MsG0580026726.01	MsG0580029236.01	0.807454	5.015424e-50	2.794755e-47
MsG0580026726.01	MsG0580029826.01	0.825378	4.843379e-54	4.406974e-51
MsG0580026726.01	MsG0680030597.01	0.822753	2.001307e-53	1.690267e-50
MsG0580026726.01	MsG0680030880.01	0.831366	1.738657e-55	1.882729e-52
MsG0580026726.01	MsG0680030882.01	0.838197	3.318558e-57	4.412003e-54
MsG0580026726.01	MsG0680031087.01	0.817661	2.937081e-52	2.152075e-49
MsG0580026726.01	MsG0680031262.01	0.806398	8.388156e-50	4.548064e-47
MsG0580026726.01	MsG0680031506.01	0.803781	2.958830e-49	1.500054e-46
MsG0580026726.01	MsG0680032157.01	0.800290	1.544898e-48	7.166641e-46
MsG0580026726.01	MsG0680032771.01	0.803680	3.106127e-49	1.570576e-46
MsG0580026726.01	MsG0680033099.01	0.800429	1.447121e-48	6.736937e-46
MsG0580026726.01	MsG0680033412.01	0.834972	2.199918e-56	2.652943e-53
MsG0580026726.01	MsG0680033465.01	0.800478	1.414568e-48	6.593078e-46
MsG0580026726.01	MsG0680033928.01	0.811297	7.513039e-51	4.633091e-48
MsG0580026726.01	MsG0680034320.01	0.808782	2.614583e-50	1.508399e-47
MsG0580026726.01	MsG0680034739.01	0.803184	3.935056e-49	1.964600e-46
MsG0580026726.01	MsG0680035573.01	0.801916	7.183044e-49	3.472137e-46
MsG0580026726.01	MsG0680035580.01	0.832794	7.712843e-56	8.712635e-53
MsG0580026726.01	MsG0680035692.01	0.809378	1.949446e-50	1.142779e-47
MsG0580026726.01	MsG0680035844.01	0.800935	1.140932e-48	5.380153e-46
MsG0580026726.01	MsG0780035948.01	0.807440	5.049895e-50	2.812841e-47
MsG0580026726.01	MsG0780036018.01	0.817669	2.925450e-52	2.144036e-49
MsG0580026726.01	MsG0780036080.01	0.854636	1.078383e-61	2.424232e-58
MsG0580026726.01	MsG0780036183.01	0.804143	2.488816e-49	1.273550e-46
MsG0580026726.01	MsG0780036190.01	0.804803	1.812912e-49	9.436311e-47
MsG0580026726.01	MsG0780036201.01	0.826307	2.914503e-54	2.723099e-51
MsG0580026726.01	MsG0780036602.01	0.805241	1.467762e-49	7.725923e-47
MsG0580026726.01	MsG0780037146.01	0.802070	6.680485e-49	3.241888e-46
MsG0580026726.01	MsG0780037292.01	0.843539	1.316765e-58	2.066759e-55
MsG0580026726.01	MsG0780038391.01	0.831339	1.765685e-55	1.910406e-52
MsG0580026726.01	MsG0780038401.01	0.816023	6.845620e-52	4.796104e-49
MsG0580026726.01	MsG0780038439.01	0.840530	8.224648e-58	1.174921e-54
MsG0580026726.01	MsG0780038906.01	0.807439	5.052309e-50	2.814107e-47
MsG0580026726.01	MsG0780039170.01	0.811825	5.768509e-51	3.607381e-48
MsG0580026726.01	MsG0780039243.01	0.828344	9.472628e-55	9.389216e-52
MsG0580026726.01	MsG0780039252.01	0.811441	6.989725e-51	4.327236e-48
MsG0580026726.01	MsG0780039443.01	0.825213	5.299843e-54	4.800081e-51
MsG0580026726.01	MsG0780039520.01	0.809724	1.642322e-50	9.716616e-48
MsG0580026726.01	MsG0780039522.01	0.817210	3.711800e-52	2.686246e-49
MsG0580026726.01	MsG0780039631.01	0.809393	1.935025e-50	1.134768e-47
MsG0580026726.01	MsG0780040057.01	0.823265	1.520157e-53	1.302496e-50
MsG0580026726.01	MsG0780040074.01	0.802569	5.272593e-49	2.591309e-46
MsG0580026726.01	MsG0780040591.01	0.824234	9.012465e-54	7.936570e-51
MsG0580026726.01	MsG0780040704.01	0.808844	2.536862e-50	1.465960e-47
MsG0580026726.01	MsG0780041229.01	0.806524	7.888864e-50	4.291243e-47
MsG0580026726.01	MsG0780041416.01	0.825880	3.681874e-54	3.398730e-51
MsG0580026726.01	MsG0780041435.01	0.804587	2.011151e-49	1.040937e-46
MsG0580026726.01	MsG0780041456.01	0.841355	4.996912e-58	7.322987e-55
MsG0580026726.01	MsG0780041507.01	0.836914	7.075390e-57	9.044991e-54
MsG0580026726.01	MsG0880042161.01	0.804843	1.777819e-49	9.263540e-47
MsG0580026726.01	MsG0880042518.01	0.800697	1.276496e-48	5.982669e-46
MsG0580026726.01	MsG0880042520.01	0.815028	1.140460e-51	7.775272e-49
MsG0580026726.01	MsG0880042656.01	0.809347	1.979040e-50	1.159186e-47
MsG0580026726.01	MsG0880042698.01	0.810926	9.040043e-51	5.521061e-48
MsG0580026726.01	MsG0880042700.01	0.823791	1.145092e-53	9.959379e-51
MsG0580026726.01	MsG0880042966.01	0.817480	3.225724e-52	2.351939e-49
MsG0580026726.01	MsG0880043253.01	0.812223	4.723943e-51	2.986372e-48
MsG0580026726.01	MsG0880043480.01	0.802474	5.514487e-49	2.703734e-46
MsG0580026726.01	MsG0880043937.01	0.800284	1.549407e-48	7.186468e-46
MsG0580026726.01	MsG0880043941.01	0.811180	7.965205e-51	4.897053e-48
MsG0580026726.01	MsG0880044565.01	0.802521	5.392558e-49	2.647030e-46
MsG0580026726.01	MsG0880044573.01	0.828170	1.043058e-54	1.028727e-51
MsG0580026726.01	MsG0880044574.01	0.825791	3.867050e-54	3.560495e-51
MsG0580026726.01	MsG0880044728.01	0.800896	1.162495e-48	5.476393e-46
MsG0580026726.01	MsG0880045111.01	0.803311	3.703428e-49	1.854934e-46
MsG0580026726.01	MsG0880045362.01	0.807582	4.710694e-50	2.633467e-47
MsG0580026726.01	MsG0880045372.01	0.828731	7.639623e-55	7.657707e-52
MsG0580026726.01	MsG0880045736.01	0.834743	2.512714e-56	3.008809e-53
MsG0580026726.01	MsG0880045895.01	0.811385	7.190592e-51	4.444661e-48
MsG0580026726.01	MsG0880045896.01	0.804996	1.651681e-49	8.639672e-47
MsG0580026726.01	MsG0880045937.01	0.810117	1.351560e-50	8.078983e-48
MsG0580026726.01	MsG0880046012.01	0.803667	3.124970e-49	1.579579e-46
MsG0580026726.01	MsG0880046122.01	0.816018	6.863433e-52	4.807919e-49
MsG0580026726.01	MsG0880046425.01	0.823753	1.168961e-53	1.015642e-50
MsG0580026726.01	MsG0880047294.01	0.811441	6.991934e-51	4.328533e-48
MsG0280006491.01	MsG0580026726.01	0.818006	2.455389e-52	1.816185e-49
MsG0280006601.01	MsG0580026726.01	0.804938	1.698608e-49	8.872461e-47
MsG0280006766.01	MsG0580026726.01	0.804727	1.880287e-49	9.768047e-47
MsG0280006770.01	MsG0580026726.01	0.843433	1.405356e-58	2.198495e-55
MsG0280007529.01	MsG0580026726.01	0.823768	1.159671e-53	1.007992e-50
MsG0280007547.01	MsG0580026726.01	0.827744	1.320634e-54	1.286318e-51
MsG0280007955.01	MsG0580026726.01	0.806703	7.230742e-50	3.951299e-47
MsG0280008112.01	MsG0580026726.01	0.820250	7.575342e-53	5.962778e-50
MsG0280008130.01	MsG0580026726.01	0.807451	5.022490e-50	2.798479e-47
MsG0280008615.01	MsG0580026726.01	0.810489	1.123685e-50	6.782600e-48
MsG0280009024.01	MsG0580026726.01	0.800556	1.363405e-48	6.367481e-46
MsG0280009299.01	MsG0580026726.01	0.804787	1.826557e-49	9.503394e-47
MsG0280009383.01	MsG0580026726.01	0.837348	5.481855e-57	7.100453e-54
MsG0280009748.01	MsG0580026726.01	0.823403	1.411106e-53	1.213696e-50
MsG0280010184.01	MsG0580026726.01	0.825714	4.031216e-54	3.703456e-51
MsG0280010349.01	MsG0580026726.01	0.838037	3.648838e-57	4.828098e-54
MsG0280010443.01	MsG0580026726.01	0.835273	1.847510e-56	2.248304e-53

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026726.01.T01	MTR_0125s0060	90.769	130	12	0	1	130	1	130	3.43e-80	232
MsG0580026726.01.T01	MTR_2g035110	80.741	135	26	0	1	135	1	135	4.85e-71	209
MsG0580026726.01.T01	MTR_8g066845	60.377	106	22	3	31	135	1	87	3.01e-28	99.8
MsG0580026726.01.T01	MTR_4g012560	70.968	62	13	1	48	109	24	80	1.62e-18	74.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 24 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATTGACAATGATATTTATA+TGG	0.247121	5:-43067893	MsG0580026726.01.T01:CDS
AACAAGAAGGGGCGCATATT+TGG	0.259894	5:-43067853	MsG0580026726.01.T01:CDS
TGAATTGAAGTGTTCATATT+AGG	0.299166	5:+43067628	None:intergenic
CCGGAAGGAGAAGGTGATTA+TGG	0.342957	5:-43067604	MsG0580026726.01.T01:CDS
TTCTACCGATGTAGATTCTT+CGG	0.370531	5:-43067776	MsG0580026726.01.T01:CDS
AAGGGGCGCATATTTGGATT+AGG	0.373630	5:-43067847	MsG0580026726.01.T01:CDS
CCATAATCACCTTCTCCTTC+CGG	0.374783	5:+43067604	None:intergenic
GATATTTATATGGAAATTGT+TGG	0.391812	5:-43067883	MsG0580026726.01.T01:CDS
GTTTCTCAAACGTTTGAGTT+TGG	0.397323	5:+43067959	None:intergenic
GAGCGCTTGGAGCAAATGAT+TGG	0.408396	5:-43067661	MsG0580026726.01.T01:CDS
AGAAACGCTAAAACTGAAGA+TGG	0.499466	5:-43067683	MsG0580026726.01.T01:CDS
AAAACTGAAGATGGAGCGCT+TGG	0.500382	5:-43067674	MsG0580026726.01.T01:CDS
TGTTGGAGGCATTAACAAGA+AGG	0.515610	5:-43067866	MsG0580026726.01.T01:CDS
ATTTATATGGAAATTGTTGG+AGG	0.522832	5:-43067880	MsG0580026726.01.T01:CDS
TCATTGTCAATTTCTTCAGT+AGG	0.525205	5:+43067904	None:intergenic
AGCGCTTGGAGCAAATGATT+GGG	0.526657	5:-43067660	MsG0580026726.01.T01:CDS
TATGAACACTTCAATTCAGC+CGG	0.533653	5:-43067623	MsG0580026726.01.T01:CDS
TCAATTCAGCCGGAAGGAGA+AGG	0.553755	5:-43067613	MsG0580026726.01.T01:CDS
TCTACATCGGTAGAAATTGA+AGG	0.554924	5:+43067784	None:intergenic
GTTGGAGGCATTAACAAGAA+GGG	0.555037	5:-43067865	MsG0580026726.01.T01:CDS
TTGGAGGCATTAACAAGAAG+GGG	0.580609	5:-43067864	MsG0580026726.01.T01:CDS
TCGGTAGAAATTGAAGGCGA+TGG	0.604104	5:+43067790	None:intergenic
AACACTTCAATTCAGCCGGA+AGG	0.621409	5:-43067619	MsG0580026726.01.T01:CDS
TTTGAGAAACACTCTCAAAG+CGG	0.702666	5:-43067946	MsG0580026726.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTGACAATGATATTTATA+TGG	-	Chr5:43067650-43067669	MsG0580026726.01.T01:CDS	15.0%
!!	GATATTTATATGGAAATTGT+TGG	-	Chr5:43067660-43067679	MsG0580026726.01.T01:CDS	20.0%
!	ATTTATATGGAAATTGTTGG+AGG	-	Chr5:43067663-43067682	MsG0580026726.01.T01:CDS	25.0%
!!	TGAATTGAAGTGTTCATATT+AGG	+	Chr5:43067918-43067937	None:intergenic	25.0%
	TCATTGTCAATTTCTTCAGT+AGG	+	Chr5:43067642-43067661	None:intergenic	30.0%
!	TTTTTCCGAAGAATCTACAT+CGG	+	Chr5:43067775-43067794	None:intergenic	30.0%
	AGAAACGCTAAAACTGAAGA+TGG	-	Chr5:43067860-43067879	MsG0580026726.01.T01:CDS	35.0%
	TATGAACACTTCAATTCAGC+CGG	-	Chr5:43067920-43067939	MsG0580026726.01.T01:CDS	35.0%
	TCTACATCGGTAGAAATTGA+AGG	+	Chr5:43067762-43067781	None:intergenic	35.0%
	TTCTACCGATGTAGATTCTT+CGG	-	Chr5:43067767-43067786	MsG0580026726.01.T01:CDS	35.0%
	TTTGAGAAACACTCTCAAAG+CGG	-	Chr5:43067597-43067616	MsG0580026726.01.T01:CDS	35.0%
!	GTTTCTCAAACGTTTGAGTT+TGG	+	Chr5:43067587-43067606	None:intergenic	35.0%
	TTCGGAAAAAGTTGCTGCTA+TGG	-	Chr5:43067785-43067804	MsG0580026726.01.T01:CDS	40.0%
!!	GTTGGAGGCATTAACAAGAA+GGG	-	Chr5:43067678-43067697	MsG0580026726.01.T01:CDS	40.0%
!!	TGTTGGAGGCATTAACAAGA+AGG	-	Chr5:43067677-43067696	MsG0580026726.01.T01:CDS	40.0%
!!	TTGGAGGCATTAACAAGAAG+GGG	-	Chr5:43067679-43067698	MsG0580026726.01.T01:CDS	40.0%
	AACACTTCAATTCAGCCGGA+AGG	-	Chr5:43067924-43067943	MsG0580026726.01.T01:CDS	45.0%
	AGCGCTTGGAGCAAATGATT+GGG	-	Chr5:43067883-43067902	MsG0580026726.01.T01:CDS	45.0%
	CCATAATCACCTTCTCCTTC+CGG	+	Chr5:43067942-43067961	None:intergenic	45.0%
	GGAAAAAGTTGCTGCTATGG+AGG	-	Chr5:43067788-43067807	MsG0580026726.01.T01:CDS	45.0%
	TCGGTAGAAATTGAAGGCGA+TGG	+	Chr5:43067756-43067775	None:intergenic	45.0%
!	AAAACTGAAGATGGAGCGCT+TGG	-	Chr5:43067869-43067888	MsG0580026726.01.T01:CDS	45.0%
!	AACAAGAAGGGGCGCATATT+TGG	-	Chr5:43067690-43067709	MsG0580026726.01.T01:CDS	45.0%
!	AAGGGGCGCATATTTGGATT+AGG	-	Chr5:43067696-43067715	MsG0580026726.01.T01:CDS	45.0%
	CCGGAAGGAGAAGGTGATTA+TGG	-	Chr5:43067939-43067958	MsG0580026726.01.T01:CDS	50.0%
	GAGCGCTTGGAGCAAATGAT+TGG	-	Chr5:43067882-43067901	MsG0580026726.01.T01:CDS	50.0%
	TCAATTCAGCCGGAAGGAGA+AGG	-	Chr5:43067930-43067949	MsG0580026726.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	43067579	43067986	43067579	ID=MsG0580026726.01;Name=MsG0580026726.01
Chr5	mRNA	43067579	43067986	43067579	ID=MsG0580026726.01.T01;Parent=MsG0580026726.01;Name=MsG0580026726.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|135
Chr5	exon	43067579	43067986	43067579	ID=MsG0580026726.01.T01:exon:18153;Parent=MsG0580026726.01.T01
Chr5	CDS	43067579	43067986	43067579	ID=MsG0580026726.01.T01:cds;Parent=MsG0580026726.01.T01

Gene Sequence

>MsG0580026726.01.T01

ATGTACCAAACTCAAACGTTTGAGAAACACTCTCAAAGCGGTGAGATTAGTAGTCAACAACCTACTGAAGAAATTGACAATGATATTTATATGGAAATTGTTGGAGGCATTAACAAGAAGGGGCGCATATTTGGATTAGGATCTCAAGCTGTTGTTGTCAAAGAATCTTTGAGACCATCGCCTTCAATTTCTACCGATGTAGATTCTTCGGAAAAAGTTGCTGCTATGGAGGCTAAGATTGAAGCATTAATTGCTGAACTTGAAAAGAAAAATCTTGAACAAGAAACGCTAAAACTGAAGATGGAGCGCTTGGAGCAAATGATTGGGAATATTGTGCCTAATATGAACACTTCAATTCAGCCGGAAGGAGAAGGTGATTATGGTAACAACGAGATAGATGAACTTTAG

Protein sequence

>MsG0580026726.01.T01

MYQTQTFEKHSQSGEISSQQPTEEIDNDIYMEIVGGINKKGRIFGLGSQAVVVKESLRPSPSISTDVDSSEKVAAMEAKIEALIAELEKKNLEQETLKLKMERLEQMIGNIVPNMNTSIQPEGEGDYGNNEIDEL*