AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022985.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022985.01.T01 MTR_8g080860 97.674 86 2 0 43 128 202 287 3.98e-56 176
MsG0480022985.01.T01 MTR_8g080860 66.176 68 23 0 43 110 110 177 1.10e-24 95.5
MsG0480022985.01.T01 MTR_8g080860 55.224 67 30 0 44 110 65 131 4.49e-18 77.8
MsG0480022985.01.T01 MTR_8g080860 97.674 86 2 0 43 128 198 283 4.67e-56 176
MsG0480022985.01.T01 MTR_8g080860 66.176 68 23 0 43 110 106 173 1.24e-24 95.5
MsG0480022985.01.T01 MTR_8g080860 55.224 67 30 0 44 110 61 127 5.06e-18 77.8
MsG0480022985.01.T01 MTR_8g080340 84.884 86 13 0 43 128 86 171 1.49e-49 156
MsG0480022985.01.T01 MTR_8g092410 47.794 136 45 1 1 110 112 247 3.38e-31 114
MsG0480022985.01.T01 MTR_8g092410 54.839 62 28 0 44 105 227 288 1.48e-14 68.9
MsG0480022985.01.T01 MTR_8g080350 61.429 70 27 0 43 112 101 170 5.28e-24 95.1
MsG0480022985.01.T01 MTR_8g080350 43.478 69 39 0 44 112 171 239 1.19e-11 60.8
MsG0480022985.01.T01 MTR_8g095240 58.750 80 28 1 36 110 208 287 1.82e-22 91.3
MsG0480022985.01.T01 MTR_8g095240 56.522 69 29 1 43 110 173 241 4.01e-15 70.9
MsG0480022985.01.T01 MTR_8g095240 39.394 99 27 1 45 110 96 194 6.89e-12 61.6
MsG0480022985.01.T01 MTR_4g107460 56.164 73 32 0 39 111 142 214 6.95e-22 89.0
MsG0480022985.01.T01 MTR_4g107460 43.750 96 43 2 22 110 76 167 4.59e-16 73.2
MsG0480022985.01.T01 MTR_4g107460 41.758 91 47 2 43 127 192 282 2.65e-12 62.4
MsG0480022985.01.T01 MTR_8g092090 57.971 69 29 0 43 111 130 198 4.54e-21 85.1
MsG0480022985.01.T01 MTR_8g092090 43.269 104 29 2 38 111 72 175 9.37e-18 76.6
MsG0480022985.01.T01 MTR_8g092090 57.746 71 29 1 43 112 176 246 1.55e-16 73.2
MsG0480022985.01.T01 MTR_8g080310 61.667 60 23 0 43 102 8 67 2.72e-20 81.3
MsG0480022985.01.T01 MTR_8g080310 56.522 69 30 0 43 111 54 122 8.78e-20 79.7
MsG0480022985.01.T01 MTR_8g080310 58.621 58 24 0 43 100 100 157 1.00e-17 74.7
MsG0480022985.01.T01 MTR_3g023310 69.388 49 15 0 44 92 74 122 4.74e-17 73.2
MsG0480022985.01.T01 MTR_3g023310 39.175 97 46 2 24 110 1 94 9.03e-11 56.6
MsG0480022985.01.T01 MTR_5g459450 45.588 68 37 0 45 112 138 205 1.04e-14 69.7
MsG0480022985.01.T01 MTR_5g459450 40.506 79 44 1 33 111 152 227 4.10e-11 59.3
MsG0480022985.01.T01 MTR_6g043150 46.053 76 41 0 44 119 124 199 1.97e-14 68.6
MsG0480022985.01.T01 MTR_6g043150 45.312 64 35 0 44 107 262 325 3.57e-11 59.3
MsG0480022985.01.T01 MTR_8g088930 41.791 67 39 0 44 110 104 170 3.29e-14 68.2
MsG0480022985.01.T01 MTR_8g088930 41.791 67 39 0 44 110 104 170 3.39e-14 68.2
MsG0480022985.01.T01 MTR_8g088930 41.791 67 39 0 44 110 104 170 4.34e-14 67.8
MsG0480022985.01.T01 MTR_8g088930 41.791 67 39 0 44 110 104 170 4.42e-14 67.8
MsG0480022985.01.T01 MTR_3g097260 45.588 68 37 0 45 112 124 191 1.76e-13 66.2
MsG0480022985.01.T01 MTR_1g062740 45.455 66 36 0 44 109 142 207 2.32e-13 65.9
MsG0480022985.01.T01 MTR_8g039100 42.857 77 41 1 33 109 136 209 3.49e-13 65.5
MsG0480022985.01.T01 MTR_3g073100 42.647 68 39 0 45 112 127 194 5.76e-13 64.7
MsG0480022985.01.T01 MTR_3g073100 41.250 80 44 1 33 112 141 217 2.90e-12 62.8
MsG0480022985.01.T01 MTR_8g095170 43.590 78 41 1 33 110 114 188 6.85e-13 64.3
MsG0480022985.01.T01 MTR_8g095170 42.647 68 39 0 45 112 100 167 3.99e-12 62.4
MsG0480022985.01.T01 MTR_8g095170 44.928 69 37 1 45 112 30 98 2.42e-11 60.1
MsG0480022985.01.T01 MTR_2g078470 40.580 69 41 0 44 112 262 330 7.59e-13 64.3
MsG0480022985.01.T01 MTR_2g078470 34.615 104 45 1 39 119 165 268 1.22e-12 63.5
MsG0480022985.01.T01 MTR_2g078470 39.130 69 42 0 44 112 124 192 8.05e-12 61.2
MsG0480022985.01.T01 MTR_2g078470 32.979 94 40 1 42 112 30 123 4.13e-11 59.3
MsG0480022985.01.T01 MTR_8g095170 42.500 80 43 1 33 112 114 190 9.46e-13 63.9
MsG0480022985.01.T01 MTR_8g095170 42.647 68 39 0 45 112 100 167 4.24e-12 62.0
MsG0480022985.01.T01 MTR_8g095170 44.928 69 37 1 45 112 30 98 3.24e-11 59.7
MsG0480022985.01.T01 MTR_7g451440 35.870 92 36 1 42 110 258 349 1.56e-12 63.2
MsG0480022985.01.T01 MTR_7g451440 42.424 66 38 0 44 109 237 302 4.84e-12 62.0
MsG0480022985.01.T01 MTR_7g451440 40.323 62 37 0 42 103 304 365 3.85e-11 59.3
MsG0480022985.01.T01 MTR_3g014650 43.939 66 37 0 44 109 168 233 1.78e-12 63.2
MsG0480022985.01.T01 MTR_8g007500 32.836 134 56 3 11 110 221 354 2.01e-12 63.2
MsG0480022985.01.T01 MTR_5g058920 35.870 92 36 1 42 110 263 354 7.74e-12 61.2
MsG0480022985.01.T01 MTR_5g058920 42.424 66 38 0 44 109 242 307 1.33e-11 60.8
MsG0480022985.01.T01 MTR_4g099180 35.417 96 51 1 44 128 217 312 7.93e-12 61.2
MsG0480022985.01.T01 MTR_4g099180 34.783 92 37 1 42 110 238 329 3.72e-11 59.3
MsG0480022985.01.T01 MTR_5g010840 47.059 68 28 1 45 112 181 240 5.56e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022985.01.T01 AT1G21980 47.059 68 36 0 45 112 145 212 5.49e-16 73.6
MsG0480022985.01.T01 AT1G21980 41.250 80 44 1 33 112 159 235 7.06e-13 64.3
MsG0480022985.01.T01 AT1G60890 45.588 68 37 0 43 110 101 168 2.80e-15 71.2
MsG0480022985.01.T01 AT1G60890 45.588 68 37 0 43 110 113 180 2.89e-15 71.2
MsG0480022985.01.T01 AT1G10900 45.588 68 37 0 43 110 101 168 7.37e-15 70.1
MsG0480022985.01.T01 AT1G10900 50.000 56 27 1 33 87 136 191 3.46e-11 59.7
MsG0480022985.01.T01 AT1G10900 45.588 68 37 0 43 110 101 168 7.37e-15 70.1
MsG0480022985.01.T01 AT1G10900 50.000 56 27 1 33 87 136 191 3.46e-11 59.7
MsG0480022985.01.T01 AT1G10900 45.588 68 37 0 43 110 101 168 7.37e-15 70.1
MsG0480022985.01.T01 AT1G10900 50.000 56 27 1 33 87 136 191 3.46e-11 59.7
MsG0480022985.01.T01 AT2G26420 50.746 67 33 0 46 112 123 189 7.98e-15 70.1
MsG0480022985.01.T01 AT2G26420 41.791 67 39 0 46 112 54 120 9.14e-11 58.5
MsG0480022985.01.T01 AT2G26420 50.746 67 33 0 46 112 123 189 8.32e-15 70.1
MsG0480022985.01.T01 AT2G26420 41.791 67 39 0 46 112 54 120 9.43e-11 58.5
MsG0480022985.01.T01 AT2G26420 50.746 67 33 0 46 112 123 189 8.32e-15 70.1
MsG0480022985.01.T01 AT2G26420 41.791 67 39 0 46 112 54 120 9.43e-11 58.5
MsG0480022985.01.T01 AT3G07960 45.455 77 39 1 33 109 110 183 1.82e-14 68.9
MsG0480022985.01.T01 AT3G07960 41.176 68 40 0 45 112 96 163 4.18e-11 59.3
MsG0480022985.01.T01 AT3G07960 45.455 77 39 1 33 109 110 183 2.54e-14 68.6
MsG0480022985.01.T01 AT3G07960 41.176 68 40 0 45 112 96 163 4.71e-11 59.3
MsG0480022985.01.T01 AT3G07960 45.455 77 39 1 33 109 110 183 2.74e-14 68.6
MsG0480022985.01.T01 AT3G07960 41.176 68 40 0 45 112 96 163 4.97e-11 59.3
MsG0480022985.01.T01 AT3G07960 45.455 77 39 1 33 109 110 183 2.74e-14 68.6
MsG0480022985.01.T01 AT3G07960 41.176 68 40 0 45 112 96 163 4.97e-11 59.3
MsG0480022985.01.T01 AT1G77740 41.250 80 44 1 33 112 160 236 1.47e-13 66.2
MsG0480022985.01.T01 AT1G77740 43.284 67 38 0 45 111 146 212 1.54e-13 66.2
MsG0480022985.01.T01 AT1G77740 41.250 80 44 1 33 112 160 236 1.47e-13 66.2
MsG0480022985.01.T01 AT1G77740 43.284 67 38 0 45 111 146 212 1.54e-13 66.2
MsG0480022985.01.T01 AT2G41210 40.845 71 42 0 42 112 136 206 4.12e-13 65.1
MsG0480022985.01.T01 AT2G41210 42.857 77 41 1 33 109 153 226 5.68e-12 62.0
MsG0480022985.01.T01 AT3G56960 39.437 71 43 0 42 112 138 208 5.15e-13 64.7
MsG0480022985.01.T01 AT3G56960 44.156 77 40 1 33 109 155 228 1.69e-12 63.5
MsG0480022985.01.T01 AT3G56960 39.437 71 43 0 42 112 138 208 5.15e-13 64.7
MsG0480022985.01.T01 AT3G56960 44.156 77 40 1 33 109 155 228 1.69e-12 63.5
MsG0480022985.01.T01 AT2G35170 30.597 134 59 2 11 110 250 383 7.26e-13 64.3
MsG0480022985.01.T01 AT3G09920 46.269 67 36 0 44 110 144 210 9.61e-13 63.9
MsG0480022985.01.T01 AT3G09920 46.269 67 36 0 44 110 144 210 9.61e-13 63.9
MsG0480022985.01.T01 AT3G09920 46.269 67 36 0 44 110 144 210 9.61e-13 63.9
MsG0480022985.01.T01 AT3G09920 46.269 67 36 0 44 110 144 210 9.61e-13 63.9
MsG0480022985.01.T01 AT4G17080 35.870 92 36 1 42 110 323 414 1.11e-12 63.9
MsG0480022985.01.T01 AT4G17080 38.710 62 38 0 42 103 369 430 9.56e-11 58.2

Find 34 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAACGTCTACACTGGAAAT+TGG 0.199973 4:-79445998 MsG0480022985.01.T01:CDS
TACACTGGAAATTGGGAAAA+AGG 0.217123 4:-79445990 MsG0480022985.01.T01:CDS
GAGATGTCTACACTGGAAAT+TGG 0.297839 4:-79445862 MsG0480022985.01.T01:CDS
AATTAAGCTTCAGATGATAA+TGG 0.335808 4:+79445697 None:intergenic
AATGGACTTTCGCATGGATA+TGG 0.353378 4:-79446041 MsG0480022985.01.T01:CDS
TACGAGGGCGATTGGGTTGA+TGG 0.368273 4:-79445785 MsG0480022985.01.T01:CDS
ATTAAGCTTCAGATGATAAT+GGG 0.406796 4:+79445698 None:intergenic
TTCGATGGTTATTGGTCAAA+TGG 0.407614 4:-79445923 MsG0480022985.01.T01:CDS
AATAATAAAATGGATGGAAG+AGG 0.408648 4:-79445836 MsG0480022985.01.T01:CDS
TTCGATGGTTGCTGGTCAAA+TGG 0.420223 4:-79446059 MsG0480022985.01.T01:CDS
AAAGGGAAAGGGATGGAAAA+GGG 0.430911 4:-79445972 MsG0480022985.01.T01:CDS
TGGGTTGATGGACAAATGAT+AGG 0.434531 4:-79445773 MsG0480022985.01.T01:CDS
AAACGTCTACACTGGAAATT+GGG 0.451480 4:-79445997 MsG0480022985.01.T01:CDS
GGGATTATGAATTGGTCAAA+TGG 0.455729 4:-79445815 MsG0480022985.01.T01:CDS
GAACAATGTACGAGGGCGAT+TGG 0.457462 4:-79445793 MsG0480022985.01.T01:CDS
AAAAGGGAAAGGGATGGAAA+AGG 0.469288 4:-79445973 MsG0480022985.01.T01:CDS
ATGGAAGAGGGATTATGAAT+TGG 0.469752 4:-79445823 MsG0480022985.01.T01:CDS
AACAATGTACGAGGGCGATT+GGG 0.470740 4:-79445792 MsG0480022985.01.T01:CDS
GGAGTTTATAGATTTGCAAA+TGG 0.480472 4:-79445884 MsG0480022985.01.T01:CDS
GGAGTTTATAGATCTGCAAA+TGG 0.513797 4:-79446020 MsG0480022985.01.T01:CDS
GATGGAAAAGGGATTATGAA+TGG 0.518259 4:-79445961 MsG0480022985.01.T01:CDS
ATAATAAAATGGATGGAAGA+GGG 0.546218 4:-79445835 MsG0480022985.01.T01:CDS
GATGGACAAATGATAGGGAA+TGG 0.550634 4:-79445767 MsG0480022985.01.T01:CDS
ATGGAAAAGGGATTATGAAT+GGG 0.553565 4:-79445960 MsG0480022985.01.T01:CDS
GGGTTGATGGACAAATGATA+GGG 0.565463 4:-79445772 MsG0480022985.01.T01:CDS
AATGGACAGAAACATGGCTA+TGG 0.574469 4:-79445905 MsG0480022985.01.T01:CDS
GGGAATGGATTGATGATACC+AGG 0.577591 4:-79445752 MsG0480022985.01.T01:CDS
GCAAATGGAAACGTCTACAC+TGG 0.588126 4:-79446005 MsG0480022985.01.T01:CDS
TGGTCAAATGGACAGAAACA+TGG 0.596729 4:-79445911 MsG0480022985.01.T01:CDS
TGGTCAAATGGACTTTCGCA+TGG 0.604154 4:-79446047 MsG0480022985.01.T01:CDS
GCAAATGGAGATGTCTACAC+TGG 0.608648 4:-79445869 MsG0480022985.01.T01:CDS
AGGGATTATGAATGGGCTAA+CGG 0.635445 4:-79445953 MsG0480022985.01.T01:CDS
GTCAAATGGAACAATGTACG+AGG 0.679412 4:-79445801 MsG0480022985.01.T01:CDS
TCAAATGGAACAATGTACGA+GGG 0.718322 4:-79445800 MsG0480022985.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTGGAAAAATAATAAAA+TGG - Chr4:79445930-79445949 MsG0480022985.01.T01:CDS 10.0%
!! TGGAAAAATAATAAAATGGA+TGG - Chr4:79445934-79445953 MsG0480022985.01.T01:CDS 20.0%
! AATAATAAAATGGATGGAAG+AGG - Chr4:79445940-79445959 MsG0480022985.01.T01:CDS 25.0%
! ATAATAAAATGGATGGAAGA+GGG - Chr4:79445941-79445960 MsG0480022985.01.T01:CDS 25.0%
AAAAATGTACATAGAAAGCC+TGG + Chr4:79446045-79446064 None:intergenic 30.0%
ATGGAAAAGGGATTATGAAT+GGG - Chr4:79445816-79445835 MsG0480022985.01.T01:CDS 30.0%
GGAGTTTATAGATTTGCAAA+TGG - Chr4:79445892-79445911 MsG0480022985.01.T01:CDS 30.0%
AAACGTCTACACTGGAAATT+GGG - Chr4:79445779-79445798 MsG0480022985.01.T01:CDS 35.0%
ACACTGGAAATTGGGAAAAA+GGG - Chr4:79445787-79445806 MsG0480022985.01.T01:CDS 35.0%
ATGGAAGAGGGATTATGAAT+TGG - Chr4:79445953-79445972 MsG0480022985.01.T01:CDS 35.0%
GAAATTGGGAAAAAGGGAAA+GGG - Chr4:79445793-79445812 MsG0480022985.01.T01:CDS 35.0%
GATGGAAAAGGGATTATGAA+TGG - Chr4:79445815-79445834 MsG0480022985.01.T01:CDS 35.0%
GGAGTTTATAGATCTGCAAA+TGG - Chr4:79445756-79445775 MsG0480022985.01.T01:CDS 35.0%
GGGATTATGAATTGGTCAAA+TGG - Chr4:79445961-79445980 MsG0480022985.01.T01:CDS 35.0%
TACACTGGAAATTGGGAAAA+AGG - Chr4:79445786-79445805 MsG0480022985.01.T01:CDS 35.0%
TCAAATGGAACAATGTACGA+GGG - Chr4:79445976-79445995 MsG0480022985.01.T01:CDS 35.0%
TTCGATGGTTATTGGTCAAA+TGG - Chr4:79445853-79445872 MsG0480022985.01.T01:CDS 35.0%
! GTGATGTTTTCGATGGTTAT+TGG - Chr4:79445845-79445864 MsG0480022985.01.T01:CDS 35.0%
AAAAGGGAAAGGGATGGAAA+AGG - Chr4:79445803-79445822 MsG0480022985.01.T01:CDS 40.0%
AAAGGGAAAGGGATGGAAAA+GGG - Chr4:79445804-79445823 MsG0480022985.01.T01:CDS 40.0%
AATGGACAGAAACATGGCTA+TGG - Chr4:79445871-79445890 MsG0480022985.01.T01:CDS 40.0%
GAAACGTCTACACTGGAAAT+TGG - Chr4:79445778-79445797 MsG0480022985.01.T01:CDS 40.0%
GAGATGTCTACACTGGAAAT+TGG - Chr4:79445914-79445933 MsG0480022985.01.T01:CDS 40.0%
GATGGACAAATGATAGGGAA+TGG - Chr4:79446009-79446028 MsG0480022985.01.T01:CDS 40.0%
GGAAATTGGGAAAAAGGGAA+AGG - Chr4:79445792-79445811 MsG0480022985.01.T01:CDS 40.0%
GTCAAATGGAACAATGTACG+AGG - Chr4:79445975-79445994 MsG0480022985.01.T01:CDS 40.0%
TGGTCAAATGGACAGAAACA+TGG - Chr4:79445865-79445884 MsG0480022985.01.T01:CDS 40.0%
! AATGGACTTTCGCATGGATA+TGG - Chr4:79445735-79445754 MsG0480022985.01.T01:CDS 40.0%
!! AGGGATTATGAATGGGCTAA+CGG - Chr4:79445823-79445842 MsG0480022985.01.T01:CDS 40.0%
!! GGGTTGATGGACAAATGATA+GGG - Chr4:79446004-79446023 MsG0480022985.01.T01:CDS 40.0%
!! TGGGTTGATGGACAAATGAT+AGG - Chr4:79446003-79446022 MsG0480022985.01.T01:CDS 40.0%
AACAATGTACGAGGGCGATT+GGG - Chr4:79445984-79446003 MsG0480022985.01.T01:CDS 45.0%
GCAAATGGAAACGTCTACAC+TGG - Chr4:79445771-79445790 MsG0480022985.01.T01:CDS 45.0%
GCAAATGGAGATGTCTACAC+TGG - Chr4:79445907-79445926 MsG0480022985.01.T01:CDS 45.0%
TTCGATGGTTGCTGGTCAAA+TGG - Chr4:79445717-79445736 MsG0480022985.01.T01:CDS 45.0%
TTGGGAAAAAGGGAAAGGGA+TGG - Chr4:79445797-79445816 MsG0480022985.01.T01:CDS 45.0%
! GCTAACGGTGATGTTTTCGA+TGG - Chr4:79445838-79445857 MsG0480022985.01.T01:CDS 45.0%
! TGGTCAAATGGACTTTCGCA+TGG - Chr4:79445729-79445748 MsG0480022985.01.T01:CDS 45.0%
!! GGGAATGGATTGATGATACC+AGG - Chr4:79446024-79446043 MsG0480022985.01.T01:CDS 45.0%
!!! GTGCTATTTTCGATGGTTGC+TGG - Chr4:79445709-79445728 MsG0480022985.01.T01:CDS 45.0%
GAACAATGTACGAGGGCGAT+TGG - Chr4:79445983-79446002 MsG0480022985.01.T01:CDS 50.0%
!! TACGAGGGCGATTGGGTTGA+TGG - Chr4:79445991-79446010 MsG0480022985.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 79445706 79446092 79445706 ID=MsG0480022985.01;Name=MsG0480022985.01
Chr4 mRNA 79445706 79446092 79445706 ID=MsG0480022985.01.T01;Parent=MsG0480022985.01;Name=MsG0480022985.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|128
Chr4 exon 79445706 79446092 79445706 ID=MsG0480022985.01.T01:exon:3904;Parent=MsG0480022985.01.T01
Chr4 CDS 79445706 79446092 79445706 ID=MsG0480022985.01.T01:cds;Parent=MsG0480022985.01.T01
Gene Sequence

>MsG0480022985.01.T01

ATGGTGCTATTTTCGATGGTTGCTGGTCAAATGGACTTTCGCATGGATATGGAGTTTATAGATCTGCAAATGGAAACGTCTACACTGGAAATTGGGAAAAAGGGAAAGGGATGGAAAAGGGATTATGAATGGGCTAACGGTGATGTTTTCGATGGTTATTGGTCAAATGGACAGAAACATGGCTATGGAGTTTATAGATTTGCAAATGGAGATGTCTACACTGGAAATTGGAAAAATAATAAAATGGATGGAAGAGGGATTATGAATTGGTCAAATGGAACAATGTACGAGGGCGATTGGGTTGATGGACAAATGATAGGGAATGGATTGATGATACCAGGCTTTCTATGTACATTTTTGAAGATTATCAGACCCATTATCATCTGA

Protein sequence

>MsG0480022985.01.T01

MVLFSMVAGQMDFRMDMEFIDLQMETSTLEIGKKGKGWKRDYEWANGDVFDGYWSNGQKHGYGVYRFANGDVYTGNWKNNKMDGRGIMNWSNGTMYEGDWVDGQMIGNGLMIPGFLCTFLKIIRPIII*