AlfalfaGEDB | MsG0480019795.01

M. sativa cultivar ZhongmuNo.1 / MsG0480019795.01

KEGG: -
GO: GO:0005515
PFAM: PF00254

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019795.01.T01	XP_024638163.1	97.71	262	6	0	1	262	111	372	0	537

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019795.01.T01	Q9LDC0	72.624	263	67	2	1	261	106	365	1.99E-138	397

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019795.01.T01	G7JTJ9	97.710	262	6	0	1	262	111	372	0.0	537

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047869.01	MsG0480019795.01	0.802150	6.431319e-49	3.127329e-46
MsG0080048404.01	MsG0480019795.01	0.809041	2.301631e-50	1.337087e-47
MsG0080048618.01	MsG0480019795.01	0.829036	6.442775e-55	6.515921e-52
MsG0180000047.01	MsG0480019795.01	0.813185	2.909317e-51	1.886718e-48
MsG0180000444.01	MsG0480019795.01	0.822917	1.832432e-53	1.554807e-50
MsG0180000470.01	MsG0480019795.01	0.810979	8.802851e-51	5.384118e-48
MsG0180000526.01	MsG0480019795.01	0.813562	2.403403e-51	1.574805e-48
MsG0180000550.01	MsG0480019795.01	0.840128	1.047569e-57	1.478027e-54
MsG0180000650.01	MsG0480019795.01	0.838608	2.598864e-57	3.499244e-54
MsG0180001063.01	MsG0480019795.01	0.841288	5.203088e-58	7.608834e-55
MsG0180002482.01	MsG0480019795.01	0.836023	1.193142e-56	1.484834e-53
MsG0180003651.01	MsG0480019795.01	0.807338	5.308336e-50	2.949000e-47
MsG0180003952.01	MsG0480019795.01	0.830902	2.261202e-55	2.415194e-52
MsG0180004264.01	MsG0480019795.01	0.800588	1.342977e-48	6.277201e-46
MsG0180004389.01	MsG0480019795.01	0.815739	7.921645e-52	5.507272e-49
MsG0180004463.01	MsG0480019795.01	0.832306	1.019236e-55	1.134594e-52
MsG0180004765.01	MsG0480019795.01	0.832842	7.506603e-56	8.491526e-53
MsG0180005763.01	MsG0480019795.01	0.832884	7.326825e-56	8.298890e-53
MsG0180006144.01	MsG0480019795.01	0.829845	4.098540e-55	4.245564e-52
MsG0180006220.01	MsG0480019795.01	0.804665	1.937487e-49	1.004827e-46
MsG0180006257.01	MsG0480019795.01	0.815215	1.036255e-51	7.100273e-49
MsG0280006299.01	MsG0480019795.01	0.801356	9.359293e-49	4.460467e-46
MsG0280010722.01	MsG0480019795.01	0.814184	1.753604e-51	1.168568e-48
MsG0280010778.01	MsG0480019795.01	0.802586	5.228468e-49	2.570832e-46
MsG0280011175.01	MsG0480019795.01	0.846918	1.605789e-59	2.806879e-56
MsG0280011305.01	MsG0480019795.01	0.800700	1.274571e-48	5.974060e-46
MsG0380011581.01	MsG0480019795.01	0.813673	2.271869e-51	1.493140e-48
MsG0380011609.01	MsG0480019795.01	0.817876	2.626086e-52	1.935588e-49
MsG0380012460.01	MsG0480019795.01	0.806161	9.410132e-50	5.071758e-47
MsG0380013853.01	MsG0480019795.01	0.804746	1.863285e-49	9.684506e-47
MsG0380014173.01	MsG0480019795.01	0.801934	7.123025e-49	3.444644e-46
MsG0380014537.01	MsG0480019795.01	0.812649	3.812074e-51	2.437258e-48
MsG0480018603.01	MsG0480019795.01	0.828936	6.812478e-55	6.869739e-52
MsG0480018606.01	MsG0480019795.01	0.830142	3.468612e-55	3.623394e-52
MsG0480018779.01	MsG0480019795.01	0.830703	2.529979e-55	2.686388e-52
MsG0480019125.01	MsG0480019795.01	0.811860	5.667985e-51	3.547775e-48
MsG0480019337.01	MsG0480019795.01	0.811835	5.738232e-51	3.589391e-48
MsG0480019795.01	MsG0480020025.01	0.812530	4.047153e-51	2.579603e-48
MsG0480019795.01	MsG0480020136.01	0.814423	1.552443e-51	1.041300e-48
MsG0480019795.01	MsG0480020283.01	0.813287	2.763086e-51	1.796951e-48
MsG0480019795.01	MsG0480020836.01	0.816497	5.363985e-52	3.806750e-49
MsG0480019795.01	MsG0480020874.01	0.822347	2.487516e-53	2.076718e-50
MsG0480019795.01	MsG0480020887.01	0.821692	3.526490e-53	2.889620e-50
MsG0480019795.01	MsG0480021164.01	0.802816	4.688907e-49	2.319185e-46
MsG0480019795.01	MsG0480021536.01	0.806151	9.454736e-50	5.094449e-47
MsG0480019795.01	MsG0480021869.01	0.813132	2.988125e-51	1.935062e-48
MsG0480019795.01	MsG0480022068.01	0.806545	7.810572e-50	4.251002e-47
MsG0480019795.01	MsG0480022222.01	0.815727	7.972968e-52	5.541043e-49
MsG0480019795.01	MsG0480022259.01	0.813288	2.761327e-51	1.795871e-48
MsG0480019795.01	MsG0480022262.01	0.806731	7.133101e-50	3.900846e-47
MsG0480019795.01	MsG0480022707.01	0.857135	2.005255e-62	4.899361e-59
MsG0480019795.01	MsG0480022855.01	0.817005	4.127209e-52	2.970341e-49
MsG0480019795.01	MsG0480022877.01	0.825429	4.710046e-54	4.291779e-51
MsG0480019795.01	MsG0480023163.01	0.800497	1.401607e-48	6.536054e-46
MsG0480019795.01	MsG0480023251.01	0.814752	1.313104e-51	8.885419e-49
MsG0480019795.01	MsG0480023463.01	0.812425	4.266688e-51	2.712077e-48
MsG0480019795.01	MsG0480023488.01	0.810974	8.824779e-51	5.396743e-48
MsG0480019795.01	MsG0480023489.01	0.844190	8.815279e-59	1.412250e-55
MsG0480019795.01	MsG0480023503.01	0.800755	1.241814e-48	5.828741e-46
MsG0480019795.01	MsG0480023636.01	0.842635	2.293096e-58	3.497925e-55
MsG0480019795.01	MsG0480023776.01	0.804445	2.152914e-49	1.110227e-46
MsG0480019795.01	MsG0580024382.01	0.817674	2.917906e-52	2.138781e-49
MsG0480019795.01	MsG0580024495.01	0.800244	1.578552e-48	7.313952e-46
MsG0480019795.01	MsG0580024552.01	0.803862	2.846399e-49	1.446102e-46
MsG0480019795.01	MsG0580025799.01	0.808826	2.559323e-50	1.478230e-47
MsG0480019795.01	MsG0580025816.01	0.800778	1.228429e-48	5.769285e-46
MsG0480019795.01	MsG0580026131.01	0.802905	4.494203e-49	2.227840e-46
MsG0480019795.01	MsG0580026726.01	0.806663	7.373514e-50	4.025015e-47
MsG0480019795.01	MsG0580027361.01	0.805035	1.621338e-49	8.489213e-47
MsG0480019795.01	MsG0580028418.01	0.808922	2.441318e-50	1.413672e-47
MsG0480019795.01	MsG0580028634.01	0.818070	2.374593e-52	1.759451e-49
MsG0480019795.01	MsG0580029008.01	0.828737	7.613301e-55	7.632707e-52
MsG0480019795.01	MsG0580029549.01	0.847817	9.094248e-60	1.636102e-56
MsG0480019795.01	MsG0680030880.01	0.813204	2.881820e-51	1.869869e-48
MsG0480019795.01	MsG0680030882.01	0.817887	2.611268e-52	1.925215e-49
MsG0480019795.01	MsG0680030992.01	0.809133	2.199798e-50	1.281130e-47
MsG0480019795.01	MsG0680031488.01	0.817607	3.021214e-52	2.210358e-49
MsG0480019795.01	MsG0680032204.01	0.820126	8.085080e-53	6.343033e-50
MsG0480019795.01	MsG0680032771.01	0.802154	6.417904e-49	3.121165e-46
MsG0480019795.01	MsG0680032996.01	0.813549	2.419481e-51	1.584865e-48
MsG0480019795.01	MsG0680033099.01	0.830305	3.165786e-55	3.322406e-52
MsG0480019795.01	MsG0680034230.01	0.806964	6.368279e-50	3.503597e-47
MsG0480019795.01	MsG0680035580.01	0.816918	4.315972e-52	3.098687e-49
MsG0480019795.01	MsG0780035948.01	0.800122	1.671412e-48	7.720348e-46
MsG0480019795.01	MsG0780036190.01	0.818172	2.251965e-52	1.673323e-49
MsG0480019795.01	MsG0780036965.01	0.802944	4.412199e-49	2.189254e-46
MsG0480019795.01	MsG0780037292.01	0.820915	5.330167e-53	4.273881e-50
MsG0480019795.01	MsG0780038391.01	0.806194	9.261917e-50	4.996026e-47
MsG0480019795.01	MsG0780038401.01	0.827953	1.176554e-54	1.153061e-51
MsG0480019795.01	MsG0780038495.01	0.806665	7.368447e-50	4.022378e-47
MsG0480019795.01	MsG0780039243.01	0.803721	3.045008e-49	1.541326e-46
MsG0480019795.01	MsG0780040057.01	0.812484	4.141485e-51	2.636565e-48
MsG0480019795.01	MsG0780040596.01	0.820184	7.842443e-53	6.162062e-50
MsG0480019795.01	MsG0780040604.01	0.805415	1.349677e-49	7.136229e-47
MsG0480019795.01	MsG0780041416.01	0.810913	9.100554e-51	5.555941e-48
MsG0480019795.01	MsG0780041435.01	0.808663	2.772354e-50	1.594444e-47
MsG0480019795.01	MsG0780041456.01	0.846115	2.660644e-59	4.531554e-56
MsG0480019795.01	MsG0780041475.01	0.821781	3.364243e-53	2.763663e-50
MsG0480019795.01	MsG0780041507.01	0.852361	4.854101e-61	1.011799e-57
MsG0480019795.01	MsG0880041836.01	0.824281	8.786521e-54	7.747354e-51
MsG0480019795.01	MsG0880042031.01	0.814537	1.464730e-51	9.854654e-49
MsG0480019795.01	MsG0880042128.01	0.808148	3.570543e-50	2.026107e-47
MsG0480019795.01	MsG0880042129.01	0.819582	1.076556e-52	8.318044e-50
MsG0480019795.01	MsG0880042656.01	0.805109	1.564366e-49	8.206659e-47
MsG0480019795.01	MsG0880042700.01	0.816985	4.169207e-52	2.999024e-49
MsG0480019795.01	MsG0880043253.01	0.824133	9.519063e-54	8.358569e-51
MsG0480019795.01	MsG0880044574.01	0.817087	3.954250e-52	2.852131e-49
MsG0480019795.01	MsG0880044728.01	0.823184	1.588225e-53	1.357718e-50
MsG0480019795.01	MsG0880045765.01	0.817778	2.764460e-52	2.032099e-49
MsG0480019795.01	MsG0880045895.01	0.809028	2.317054e-50	1.345560e-47
MsG0480019795.01	MsG0880045896.01	0.805804	1.118299e-49	5.972418e-47
MsG0480019795.01	MsG0880045937.01	0.819099	1.387261e-52	1.057555e-49
MsG0480019795.01	MsG0880046122.01	0.832174	1.099064e-55	1.218800e-52
MsG0480019795.01	MsG0880046620.01	0.814633	1.394729e-51	9.408170e-49
MsG0480019795.01	MsG0880047294.01	0.808685	2.743305e-50	1.578614e-47
MsG0380015533.01	MsG0480019795.01	0.807629	4.603520e-50	2.576850e-47
MsG0380015555.01	MsG0480019795.01	0.820014	8.580662e-53	6.710337e-50
MsG0380015566.01	MsG0480019795.01	0.824769	6.744430e-54	6.031864e-51
MsG0380015933.01	MsG0480019795.01	0.806299	8.798657e-50	4.758777e-47
MsG0380016004.01	MsG0480019795.01	0.811281	7.573578e-51	4.668463e-48
MsG0380016191.01	MsG0480019795.01	-0.800168	1.635433e-48	7.562870e-46
MsG0380016285.01	MsG0480019795.01	0.800568	1.356230e-48	6.335665e-46
MsG0380016407.01	MsG0480019795.01	0.807945	3.943654e-50	2.225763e-47
MsG0380016529.01	MsG0480019795.01	0.823128	1.636230e-53	1.396643e-50
MsG0380016910.01	MsG0480019795.01	0.844584	6.905022e-59	1.119915e-55
MsG0380017461.01	MsG0480019795.01	0.844230	8.600524e-59	1.379487e-55
MsG0380017498.01	MsG0480019795.01	0.826881	2.126531e-54	2.019812e-51
MsG0380017680.01	MsG0480019795.01	0.841980	3.419255e-58	5.109955e-55
MsG0380018075.01	MsG0480019795.01	0.852049	5.955905e-61	1.229238e-57
MsG0480018251.01	MsG0480019795.01	0.824143	9.467346e-54	8.315638e-51
MsG0280006491.01	MsG0480019795.01	0.822953	1.797709e-53	1.526831e-50
MsG0280006769.01	MsG0480019795.01	0.827104	1.880661e-54	1.797923e-51
MsG0280007529.01	MsG0480019795.01	0.802302	5.983766e-49	2.921174e-46
MsG0280007547.01	MsG0480019795.01	0.843922	1.040070e-58	1.652369e-55
MsG0280008112.01	MsG0480019795.01	0.800015	1.757407e-48	8.095310e-46
MsG0280009299.01	MsG0480019795.01	0.805267	1.449995e-49	7.637248e-47
MsG0280010443.01	MsG0480019795.01	0.806664	7.369102e-50	4.022723e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019795.01.T01	MTR_4g039430	97.710	262	6	0	1	262	111	372	0.0	537
MsG0480019795.01.T01	MTR_8g080980	36.486	222	135	3	1	221	327	543	3.56e-32	124
MsG0480019795.01.T01	MTR_8g012340	36.937	222	134	4	1	221	331	547	4.74e-32	124
MsG0480019795.01.T01	MTR_7g053470	34.821	224	141	2	1	224	324	542	1.30e-31	122
MsG0480019795.01.T01	MTR_1g017870	32.189	233	144	6	1	228	115	338	1.94e-26	105
MsG0480019795.01.T01	MTR_1g017870	32.751	229	140	6	1	224	115	334	4.48e-26	105
MsG0480019795.01.T01	MTR_1g017870	32.189	233	144	6	1	228	199	422	5.98e-26	105
MsG0480019795.01.T01	MTR_1g017870	32.751	229	140	6	1	224	199	418	8.47e-26	105
MsG0480019795.01.T01	MTR_1g017870	32.751	229	140	6	1	224	301	520	1.40e-25	105
MsG0480019795.01.T01	MTR_1g017870	32.751	229	140	6	1	224	325	544	1.53e-25	105
MsG0480019795.01.T01	MTR_1g017870	32.751	229	140	6	1	224	325	544	1.94e-25	105
MsG0480019795.01.T01	MTR_4g055500	30.603	232	137	7	3	225	336	552	6.47e-19	86.3
MsG0480019795.01.T01	MTR_8g010620	32.414	145	91	4	1	144	134	272	2.34e-14	72.0
MsG0480019795.01.T01	MTR_8g010620	32.414	145	91	4	1	144	201	339	4.22e-14	71.6
MsG0480019795.01.T01	MTR_8g010620	32.414	145	91	4	1	144	237	375	4.95e-14	71.6
MsG0480019795.01.T01	MTR_8g010620	32.414	145	91	4	1	144	262	400	5.24e-14	71.6
MsG0480019795.01.T01	MTR_8g011560	31.724	145	92	4	1	144	66	204	1.84e-13	68.6
MsG0480019795.01.T01	MTR_7g059150	30.303	132	82	1	71	202	92	213	6.85e-12	64.3
MsG0480019795.01.T01	MTR_8g011100	31.034	145	93	3	1	144	248	386	9.49e-12	64.7
MsG0480019795.01.T01	MTR_4g050120	30.909	165	106	1	41	205	70	226	5.76e-11	62.8
MsG0480019795.01.T01	MTR_2g090135	32.323	99	57	1	74	172	98	186	7.79e-11	60.5
MsG0480019795.01.T01	MTR_2g090135	28.682	129	82	1	74	202	98	216	8.32e-11	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019795.01.T01	AT3G21640	72.624	263	67	2	1	261	106	365	2.03e-139	397
MsG0480019795.01.T01	AT5G48570	35.217	230	139	4	1	227	339	561	2.50e-30	119
MsG0480019795.01.T01	AT3G25230	34.685	222	139	3	1	221	329	545	5.83e-30	118
MsG0480019795.01.T01	AT3G25230	34.685	222	139	3	1	221	329	545	7.17e-30	117
MsG0480019795.01.T01	AT3G54010	32.743	226	124	8	11	225	252	460	1.06e-20	91.7
MsG0480019795.01.T01	AT3G54010	32.743	226	124	8	11	225	342	550	1.12e-20	91.7
MsG0480019795.01.T01	AT1G58450	32.716	162	98	5	65	222	1	155	4.42e-13	65.9
MsG0480019795.01.T01	AT5G21990	32.414	145	90	2	60	204	89	225	5.89e-11	62.8

Find 99 sgRNAs with CRISPR-Local

Find 281 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGATGGAAATGTTCTGTTTC+AGG	0.222182	4:+27430694	MsG0480019795.01.T01:CDS
GAGATCTATAAAGGAATATT+TGG	0.225703	4:+27432460	MsG0480019795.01.T01:CDS
GCAATTTGAAGGCTTTATTT+AGG	0.233759	4:+27432287	MsG0480019795.01.T01:CDS
TCAACTTCAGAATCAACTTT+TGG	0.240967	4:-27429049	None:intergenic
TGTGGTACTGGTTCAGGTCT+TGG	0.262790	4:-27432484	None:intergenic
CTTGCAATTGCTTTATCTTC+AGG	0.277406	4:-27432385	None:intergenic
GACTTCATGTTCCAGTTGTT+TGG	0.278250	4:+27430897	MsG0480019795.01.T01:CDS
AGGTTCAAGGCTAATTTATT+TGG	0.283449	4:-27432934	None:intergenic
TCTGCCATTGGTGGAACATT+TGG	0.294220	4:-27430494	None:intergenic
GGTTACAAAACAAATTATTA+AGG	0.302747	4:+27429087	MsG0480019795.01.T01:five_prime_UTR
TTGGTAGCAATTACAAGGTT+AGG	0.313686	4:-27432881	None:intergenic
CCAGTTGTTTGGGAAGTATA+GGG	0.330723	4:+27430908	MsG0480019795.01.T01:CDS
AAATGACTGGCTTGGGCATT+GGG	0.332295	4:+27430387	MsG0480019795.01.T01:exon
CTGCGTGTGAGCTGGGAATT+AGG	0.336747	4:+27430443	MsG0480019795.01.T01:CDS
GTTTGGGAAGTATAGGGATA+TGG	0.339954	4:+27430914	MsG0480019795.01.T01:CDS
ACTTCATGTTCCAGTTGTTT+GGG	0.360386	4:+27430898	MsG0480019795.01.T01:CDS
CCCTATACTTCCCAAACAAC+TGG	0.377001	4:-27430908	None:intergenic
GGAATTGGTTCATATTTGTT+TGG	0.380589	4:+27432512	MsG0480019795.01.T01:CDS
GTTTATGAAGTTGAGCTCAT+TGG	0.387560	4:+27430521	MsG0480019795.01.T01:CDS
GGACTACTCAATAATTCTTC+AGG	0.387637	4:-27429025	None:intergenic
AAGCAGAAGGAGATCTATAA+AGG	0.387775	4:+27432451	MsG0480019795.01.T01:CDS
TCCAGTTGTTTGGGAAGTAT+AGG	0.389067	4:+27430907	MsG0480019795.01.T01:CDS
GAGCGTTGCTGCGTGTGAGC+TGG	0.407608	4:+27430435	MsG0480019795.01.T01:CDS
AAAATGTTGTAGCCTTTCAT+AGG	0.416050	4:-27432663	None:intergenic
GTGAGCTGGGAATTAGGATA+TGG	0.419661	4:+27430449	MsG0480019795.01.T01:CDS
CATATTTGTTTGGCAGTGGT+TGG	0.426113	4:+27432522	MsG0480019795.01.T01:CDS
GAAATGACTGGCTTGGGCAT+TGG	0.428307	4:+27430386	MsG0480019795.01.T01:exon
TGACTGTAGAGGAACGGATT+GGG	0.429854	4:+27430651	MsG0480019795.01.T01:CDS
ACAAAAGCATGTTGAATACT+TGG	0.433435	4:-27429131	None:intergenic
AAGAAAGAAATGACTGGCTT+GGG	0.441743	4:+27430380	MsG0480019795.01.T01:exon
GAAAGTACTGAATATGCAAA+AGG	0.441865	4:+27428995	MsG0480019795.01.T01:five_prime_UTR
ACCTTGTAATTGCTACCAAC+AGG	0.451469	4:+27432885	MsG0480019795.01.T01:three_prime_UTR
TAAACTTTAGAGAAGGTTCA+AGG	0.455714	4:-27432947	None:intergenic
ATGTCAGGCCATAGCTTACA+TGG	0.460197	4:+27430869	MsG0480019795.01.T01:intron
TGGTAGCAATTACAAGGTTA+GGG	0.460657	4:-27432880	None:intergenic
GAAGAAAGAAATGACTGGCT+TGG	0.461382	4:+27430379	MsG0480019795.01.T01:exon
GGCCATGCAACAGTATGAAA+TGG	0.476558	4:+27430733	MsG0480019795.01.T01:CDS
AAAGTACTGAATATGCAAAA+GGG	0.478686	4:+27428996	MsG0480019795.01.T01:five_prime_UTR
CAGTACCACAGAAAAGGAAT+TGG	0.480228	4:+27432497	MsG0480019795.01.T01:CDS
TTGGGCAGACAGATGTTGCC+AGG	0.480432	4:+27432332	MsG0480019795.01.T01:CDS
GGGGTGGCAAGCATGAAAGC+TGG	0.481889	4:+27430407	MsG0480019795.01.T01:CDS
GAAGTATAGGGATATGGCTC+TGG	0.484336	4:+27430920	MsG0480019795.01.T01:CDS
GGCTTTATTTAGGAGAGGTA+AGG	0.490198	4:+27432297	MsG0480019795.01.T01:CDS
GACAGGCCGCCGTGTTAAGA+TGG	0.492108	4:-27430959	None:intergenic
CTGAACCAGTACCACAGAAA+AGG	0.492954	4:+27432491	MsG0480019795.01.T01:CDS
GGGTGGCAAGCATGAAAGCT+GGG	0.494339	4:+27430408	MsG0480019795.01.T01:CDS
GGTTCATATTTGTTTGGCAG+TGG	0.498991	4:+27432518	MsG0480019795.01.T01:CDS
TTTGTTCGTGTGTGGTGTGT+GGG	0.501727	4:-27428552	None:intergenic
TCATAAACTAAATCTGCCAT+TGG	0.508037	4:-27430506	None:intergenic
TGAGGATGAGAGCAATTTGA+AGG	0.509951	4:+27432276	MsG0480019795.01.T01:CDS
GGACTCAACAGTAGTATCAA+TGG	0.517836	4:-27428531	None:intergenic
ATGACTGTAGAGGAACGGAT+TGG	0.522055	4:+27430650	MsG0480019795.01.T01:CDS
AATTCAGTTGTTGAACATCA+AGG	0.527290	4:+27432605	MsG0480019795.01.T01:exon
GCCTGTTGGTAGCAATTACA+AGG	0.529193	4:-27432886	None:intergenic
AACGGATTGGGGCAGCAGAT+CGG	0.529421	4:+27430663	MsG0480019795.01.T01:CDS
TAAACTAAATCTGCCATTGG+TGG	0.530780	4:-27430503	None:intergenic
AGCGTTGCTGCGTGTGAGCT+GGG	0.534499	4:+27430436	MsG0480019795.01.T01:CDS
GGTAAGGCTAGAGCAGCACT+TGG	0.543948	4:+27432313	MsG0480019795.01.T01:CDS
AAGCATGTTGAATACTTGGA+AGG	0.544025	4:-27429127	None:intergenic
GTCATCTCCCATGTAAGCTA+TGG	0.545323	4:-27430877	None:intergenic
GGCAGCAGATCGGAGAAAGA+TGG	0.546425	4:+27430673	MsG0480019795.01.T01:CDS
AGTTGAAGTTCTTCATGAGA+AGG	0.548105	4:+27429066	MsG0480019795.01.T01:five_prime_UTR
ACAAAACAAATTATTAAGGA+AGG	0.552128	4:+27429091	MsG0480019795.01.T01:five_prime_UTR
GCGGTTCAGCTTAATCAGAC+AGG	0.561365	4:-27430976	None:intergenic
ATGTTGTAGCCTTTCATAGG+TGG	0.562072	4:-27432660	None:intergenic
GAATTAGGATATGGAAAGGA+AGG	0.562161	4:+27430458	MsG0480019795.01.T01:CDS
GAAACTTACGTTGACCAGAT+CGG	0.566358	4:-27428577	None:intergenic
AACCGCTATGAAGAAGCTAT+AGG	0.568196	4:+27430993	MsG0480019795.01.T01:CDS
GCTAATTTATTTGGTAAACA+AGG	0.569623	4:-27432925	None:intergenic
GACTGGCTTGGGCATTGGGG+TGG	0.573654	4:+27430391	MsG0480019795.01.T01:CDS
CAAATTATTAAGGAAGGTCA+TGG	0.574339	4:+27429097	MsG0480019795.01.T01:five_prime_UTR
AACCATTTCATACTGTTGCA+TGG	0.576813	4:-27430735	None:intergenic
ACAGAGAAGAAAGAAATGAC+TGG	0.578132	4:+27430374	MsG0480019795.01.T01:intron
GACTGTAGAGGAACGGATTG+GGG	0.579307	4:+27430652	MsG0480019795.01.T01:CDS
TTGAAGGCTTTATTTAGGAG+AGG	0.585733	4:+27432292	MsG0480019795.01.T01:CDS
CTGGGAATTAGGATATGGAA+AGG	0.597900	4:+27430454	MsG0480019795.01.T01:CDS
GCAGATCGGAGAAAGATGGA+TGG	0.599142	4:+27430677	MsG0480019795.01.T01:CDS
GCCGCCGTGTTAAGATGGCA+TGG	0.599939	4:-27430954	None:intergenic
GTTTCAGGAAAATAAACTAG+AGG	0.610535	4:+27430709	MsG0480019795.01.T01:CDS
TCAGGAAAATAAACTAGAGG+AGG	0.614029	4:+27430712	MsG0480019795.01.T01:CDS
AAGTACTGAATATGCAAAAG+GGG	0.616531	4:+27428997	MsG0480019795.01.T01:five_prime_UTR
TATGTGCATCCACCTATGAA+AGG	0.617546	4:+27432651	MsG0480019795.01.T01:three_prime_UTR
AGCTATTTATCAAAAGCAGA+AGG	0.618624	4:+27432438	MsG0480019795.01.T01:CDS
GTCCTATAGCTTCTTCATAG+CGG	0.623004	4:-27430995	None:intergenic
AGCGAGAGTAACAACAGCGA+GGG	0.624094	4:-27428497	None:intergenic
CATGTCAGTTACATGCCTGT+TGG	0.625735	4:-27432900	None:intergenic
AACAATAGTCGATGCAACAA+TGG	0.626001	4:-27432628	None:intergenic
GTAAGGCTAGAGCAGCACTT+GGG	0.626696	4:+27432314	MsG0480019795.01.T01:CDS
AAACTTGCAGGTATTAAGTG+AGG	0.627657	4:+27432258	MsG0480019795.01.T01:intron
AATGACTGGCTTGGGCATTG+GGG	0.631535	4:+27430388	MsG0480019795.01.T01:exon
GTGATATGACTGTAGAGGAA+CGG	0.631871	4:+27430645	MsG0480019795.01.T01:CDS
TCGCAGTGATATGACTGTAG+AGG	0.641089	4:+27430640	MsG0480019795.01.T01:CDS
TGGGCAGACAGATGTTGCCA+GGG	0.643676	4:+27432333	MsG0480019795.01.T01:CDS
GAATCCATGCCATCTTAACA+CGG	0.645723	4:+27430950	MsG0480019795.01.T01:CDS
GGTGGCAAGCATGAAAGCTG+GGG	0.672895	4:+27430409	MsG0480019795.01.T01:CDS
TGTCAGGCCATAGCTTACAT+GGG	0.675965	4:+27430870	MsG0480019795.01.T01:intron
TGTCAAACACGATTACTCTG+TGG	0.727679	4:-27432768	None:intergenic
TAGCGAGAGTAACAACAGCG+AGG	0.732800	4:-27428498	None:intergenic
TCCATGCCATCTTAACACGG+CGG	0.771881	4:+27430953	MsG0480019795.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAAAAATATAAAATTAA+TGG	-	Chr4:27428677-27428696	None:intergenic	0.0%
!!	AAAAAAAATATAAAATTAAT+GGG	-	Chr4:27428676-27428695	None:intergenic	0.0%
!!	CTATAATAAAATTATAATCA+TGG	+	Chr4:27431518-27431537	MsG0480019795.01.T01:intron	10.0%
!!!	TAAATTGTTATAGTATTTTC+TGG	+	Chr4:27432169-27432188	MsG0480019795.01.T01:intron	15.0%
!!!	TGTATATTTATTTTAGTTCA+TGG	+	Chr4:27429808-27429827	MsG0480019795.01.T01:intron	15.0%
!!	ACAAAACAAATTATTAAGGA+AGG	+	Chr4:27429091-27429110	MsG0480019795.01.T01:five_prime_UTR	20.0%
!!	ACTAAAATAAATATACACTC+AGG	-	Chr4:27429805-27429824	None:intergenic	20.0%
!!	ATAAATGGAAATTAATTACC+TGG	-	Chr4:27428744-27428763	None:intergenic	20.0%
!!	CTAAAATAAATATACACTCA+GGG	-	Chr4:27429804-27429823	None:intergenic	20.0%
!!	CTTGATAAAAGTTTATAAAG+AGG	+	Chr4:27429974-27429993	MsG0480019795.01.T01:intron	20.0%
!!	GGTTACAAAACAAATTATTA+AGG	+	Chr4:27429087-27429106	MsG0480019795.01.T01:five_prime_UTR	20.0%
!!	TAAAATAAATATACACTCAG+GGG	-	Chr4:27429803-27429822	None:intergenic	20.0%
!!	TAACAATTTACAAGTATGTT+TGG	-	Chr4:27432159-27432178	None:intergenic	20.0%
!!	TACTATGTAAATAAGCATTT+CGG	+	Chr4:27431066-27431085	MsG0480019795.01.T01:intron	20.0%
!!	TTATAAACTGGTAATAGAAA+TGG	+	Chr4:27429691-27429710	MsG0480019795.01.T01:intron	20.0%
!!	TTCAACAAGAGAAAATAAAA+AGG	-	Chr4:27429437-27429456	None:intergenic	20.0%
!!!	AGAGTAAATTTTCATTTTAG+TGG	+	Chr4:27429729-27429748	MsG0480019795.01.T01:intron	20.0%
!!!	ATTCCTTTTTATCTTATTGA+AGG	+	Chr4:27431626-27431645	MsG0480019795.01.T01:intron	20.0%
!!!	ATTGGTCTTTATTAATGTAA+GGG	-	Chr4:27431149-27431168	None:intergenic	20.0%
!!!	CAGAGATTTTTATGTAATTT+TGG	+	Chr4:27429890-27429909	MsG0480019795.01.T01:intron	20.0%
!!!	TAAAGAAATATTTTGATCGA+GGG	-	Chr4:27431181-27431200	None:intergenic	20.0%
!!!	TTCCTTTTTATCTTATTGAA+GGG	+	Chr4:27431627-27431646	MsG0480019795.01.T01:intron	20.0%
!	AAAGAAATCCAATTGATCAA+TGG	-	Chr4:27429626-27429645	None:intergenic	25.0%
!	AACAAAAGAAAATTTCCTCA+CGG	-	Chr4:27429306-27429325	None:intergenic	25.0%
!	ACAATTTAGAGAATAGATGT+AGG	-	Chr4:27429659-27429678	None:intergenic	25.0%
!	ACTAAATAAAAGTGAGAATG+AGG	-	Chr4:27432074-27432093	None:intergenic	25.0%
!	ACTATCAAAAGAGATATTTG+AGG	+	Chr4:27429840-27429859	MsG0480019795.01.T01:intron	25.0%
!	AGAAAAACTTGAATCAAACA+TGG	-	Chr4:27431912-27431931	None:intergenic	25.0%
!	AGATATAGAATCATGTTACA+AGG	-	Chr4:27432204-27432223	None:intergenic	25.0%
!	AGCATAAATTTGAAGATACA+TGG	+	Chr4:27429527-27429546	MsG0480019795.01.T01:intron	25.0%
!	ATTAATGGGAAAATGAAATG+TGG	-	Chr4:27428662-27428681	None:intergenic	25.0%
!	ATTCATAATTATGACCTGTA+TGG	+	Chr4:27429461-27429480	MsG0480019795.01.T01:intron	25.0%
!	ATTTCCCTTGACTAAAAATA+TGG	-	Chr4:27431755-27431774	None:intergenic	25.0%
!	CAAATTTGATTATCTACCAT+TGG	+	Chr4:27431283-27431302	MsG0480019795.01.T01:intron	25.0%
!	GAAAAACTTGAATCAAACAT+GGG	-	Chr4:27431911-27431930	None:intergenic	25.0%
!	GAGATCTATAAAGGAATATT+TGG	+	Chr4:27432460-27432479	MsG0480019795.01.T01:CDS	25.0%
!	GATAAAAGTTTATAAAGAGG+AGG	+	Chr4:27429977-27429996	MsG0480019795.01.T01:intron	25.0%
!	GATACAAGACTAATATTTAC+AGG	-	Chr4:27430125-27430144	None:intergenic	25.0%
!	GCTAATTTATTTGGTAAACA+AGG	-	Chr4:27432928-27432947	None:intergenic	25.0%
!	TATAGTGAGTAAAATTCACA+GGG	+	Chr4:27430611-27430630	MsG0480019795.01.T01:intron	25.0%
!	TGGTAGATAATCAAATTTGA+AGG	-	Chr4:27431282-27431301	None:intergenic	25.0%
!	TTATAGTGAGTAAAATTCAC+AGG	+	Chr4:27430610-27430629	MsG0480019795.01.T01:intron	25.0%
!	TTCCCTTCAATAAGATAAAA+AGG	-	Chr4:27431632-27431651	None:intergenic	25.0%
!!	AAATGTGGTTATGAATTTTC+CGG	-	Chr4:27428647-27428666	None:intergenic	25.0%
!!	AATTGCTGAAGAAAATTTTG+AGG	-	Chr4:27431093-27431112	None:intergenic	25.0%
!!	ATTTTATCTATGCTTGATGT+TGG	+	Chr4:27432717-27432736	MsG0480019795.01.T01:three_prime_UTR	25.0%
!!	GTTTTCATTAAATTGTTGCA+TGG	+	Chr4:27430567-27430586	MsG0480019795.01.T01:intron	25.0%
!!	TGATTTTCGTGTTTATAAAC+TGG	+	Chr4:27429679-27429698	MsG0480019795.01.T01:intron	25.0%
!!	TTTGTTCATAAGCATTTTGT+GGG	+	Chr4:27432822-27432841	MsG0480019795.01.T01:three_prime_UTR	25.0%
!!	TTTTCTTGACCTACAAAATA+AGG	-	Chr4:27428953-27428972	None:intergenic	25.0%
!!!	AAAGTACTGAATATGCAAAA+GGG	+	Chr4:27428996-27429015	MsG0480019795.01.T01:five_prime_UTR	25.0%
!!!	AAATTGTTTTACCGAGTTTT+GGG	+	Chr4:27431486-27431505	MsG0480019795.01.T01:intron	25.0%
!!!	ATGCTTGAAGTTTAAAGTTT+TGG	+	Chr4:27429589-27429608	MsG0480019795.01.T01:intron	25.0%
!!!	ATGTAATTTTGGTACATTCA+AGG	+	Chr4:27429901-27429920	MsG0480019795.01.T01:intron	25.0%
!!!	CTAAAGAAATATTTTGATCG+AGG	-	Chr4:27431182-27431201	None:intergenic	25.0%
!!!	GATTGGTCTTTATTAATGTA+AGG	-	Chr4:27431150-27431169	None:intergenic	25.0%
!!!	TAAATTGTTTTACCGAGTTT+TGG	+	Chr4:27431485-27431504	MsG0480019795.01.T01:intron	25.0%
!!!	TATTTTTTTTTCCAGCAACA+TGG	+	Chr4:27428686-27428705	MsG0480019795.01.T01:intron	25.0%
!!!	TCTTTAACAGACTTGTTATT+TGG	+	Chr4:27430331-27430350	MsG0480019795.01.T01:intron	25.0%
!!!	TTGCTATTTCACAAATTTGA+GGG	-	Chr4:27431120-27431139	None:intergenic	25.0%
!!!	TTTGCTATTTCACAAATTTG+AGG	-	Chr4:27431121-27431140	None:intergenic	25.0%
	AAAATGTTGTAGCCTTTCAT+AGG	-	Chr4:27432666-27432685	None:intergenic	30.0%
	AAATGAGTGAATTATATCCC+TGG	-	Chr4:27431963-27431982	None:intergenic	30.0%
	AAATGTCTCACTCTTTATCA+TGG	-	Chr4:27432003-27432022	None:intergenic	30.0%
	AATGAGTGAATTATATCCCT+GGG	-	Chr4:27431962-27431981	None:intergenic	30.0%
	AATGTCTCACTCTTTATCAT+GGG	-	Chr4:27432002-27432021	None:intergenic	30.0%
	AATTCAGTTGTTGAACATCA+AGG	+	Chr4:27432605-27432624	MsG0480019795.01.T01:exon	30.0%
	AGCTATTTATCAAAAGCAGA+AGG	+	Chr4:27432438-27432457	MsG0480019795.01.T01:CDS	30.0%
	AGGTTCAAGGCTAATTTATT+TGG	-	Chr4:27432937-27432956	None:intergenic	30.0%
	ATTAAATTGTTGCATGGTCA+CGG	+	Chr4:27430573-27430592	MsG0480019795.01.T01:intron	30.0%
	ATTATGACCTGTATGGTTTA+TGG	+	Chr4:27429468-27429487	MsG0480019795.01.T01:intron	30.0%
	ATTGAGATGGTAATAGGAAA+AGG	+	Chr4:27429565-27429584	MsG0480019795.01.T01:intron	30.0%
	CAAATTATTAAGGAAGGTCA+TGG	+	Chr4:27429097-27429116	MsG0480019795.01.T01:five_prime_UTR	30.0%
	CTGTCATTGTGTTGAAATTT+GGG	-	Chr4:27430045-27430064	None:intergenic	30.0%
	GAGTATAGAAGTCAATATGT+AGG	-	Chr4:27431232-27431251	None:intergenic	30.0%
	GGTAAGTGAAAAGTTATAGT+TGG	-	Chr4:27432053-27432072	None:intergenic	30.0%
	GTATCACATGAGTGAATAAA+TGG	-	Chr4:27428759-27428778	None:intergenic	30.0%
	GTTTCAGGAAAATAAACTAG+AGG	+	Chr4:27430709-27430728	MsG0480019795.01.T01:CDS	30.0%
	TAAAATTTCTGCGATTTCCT+GGG	+	Chr4:27431935-27431954	MsG0480019795.01.T01:intron	30.0%
	TAAATTTGAAGATACATGGC+AGG	+	Chr4:27429531-27429550	MsG0480019795.01.T01:intron	30.0%
	TCAATATGTAGGTCTTTCAT+CGG	-	Chr4:27431221-27431240	None:intergenic	30.0%
	TCATAAACTAAATCTGCCAT+TGG	-	Chr4:27430509-27430528	None:intergenic	30.0%
	TCGAATTTCATGTATGATTG+AGG	+	Chr4:27428924-27428943	MsG0480019795.01.T01:intron	30.0%
	TGTCATTGTGTTGAAATTTG+GGG	-	Chr4:27430044-27430063	None:intergenic	30.0%
	TTAAAATTTCTGCGATTTCC+TGG	+	Chr4:27431934-27431953	MsG0480019795.01.T01:intron	30.0%
	TTATCATGGGAATGAAAAAG+CGG	-	Chr4:27431989-27432008	None:intergenic	30.0%
	TTTCAACAAATGACTTCTGT+TGG	-	Chr4:27432241-27432260	None:intergenic	30.0%
	TTTCCTATTACCATCTCAAT+TGG	-	Chr4:27429565-27429584	None:intergenic	30.0%
!	ACAAAAGCATGTTGAATACT+TGG	-	Chr4:27429134-27429153	None:intergenic	30.0%
!	ATTCAACATGCTTTTGTAAG+TGG	+	Chr4:27429137-27429156	MsG0480019795.01.T01:intron	30.0%
!	ATTTTTACCAAAGTGAGCTT+TGG	+	Chr4:27431874-27431893	MsG0480019795.01.T01:intron	30.0%
!	GGAATTGGTTCATATTTGTT+TGG	+	Chr4:27432512-27432531	MsG0480019795.01.T01:CDS	30.0%
!	GTTTGTTCATAAGCATTTTG+TGG	+	Chr4:27432821-27432840	MsG0480019795.01.T01:three_prime_UTR	30.0%
!	TCAACTTCAGAATCAACTTT+TGG	-	Chr4:27429052-27429071	None:intergenic	30.0%
!	TTTGAATGACCCAAATGTTT+TGG	+	Chr4:27429276-27429295	MsG0480019795.01.T01:intron	30.0%
!!	AAGTACTGAATATGCAAAAG+GGG	+	Chr4:27428997-27429016	MsG0480019795.01.T01:five_prime_UTR	30.0%
!!	CTTTTGTTTAGACTGAATGT+GGG	+	Chr4:27429318-27429337	MsG0480019795.01.T01:intron	30.0%
!!	GAAAGTACTGAATATGCAAA+AGG	+	Chr4:27428995-27429014	MsG0480019795.01.T01:five_prime_UTR	30.0%
!!	GCAATTTGAAGGCTTTATTT+AGG	+	Chr4:27432287-27432306	MsG0480019795.01.T01:CDS	30.0%
!!	GTTTGCTAAGTTGATTATCT+TGG	+	Chr4:27430843-27430862	MsG0480019795.01.T01:intron	30.0%
!!	TCTTTTGTTTAGACTGAATG+TGG	+	Chr4:27429317-27429336	MsG0480019795.01.T01:intron	30.0%
!!!	CATCTTTGTGCTTTTTTTCA+CGG	-	Chr4:27432582-27432601	None:intergenic	30.0%
!!!	CTTGCCATATTTTTAGTCAA+GGG	+	Chr4:27431748-27431767	MsG0480019795.01.T01:intron	30.0%
!!!	GACTTGTTATTTGGTTTTGA+TGG	+	Chr4:27430340-27430359	MsG0480019795.01.T01:intron	30.0%
!!!	TAATTACCTGGTTTTGATTG+TGG	-	Chr4:27428732-27428751	None:intergenic	30.0%
!!!	TCTTGCCATATTTTTAGTCA+AGG	+	Chr4:27431747-27431766	MsG0480019795.01.T01:intron	30.0%
!!!	TTGAGGAAACCTTATTTTGT+AGG	+	Chr4:27428941-27428960	MsG0480019795.01.T01:intron	30.0%
	AAACTTGCAGGTATTAAGTG+AGG	+	Chr4:27432258-27432277	MsG0480019795.01.T01:intron	35.0%
	AAAGAGTGAGACATTTCCAT+GGG	+	Chr4:27432007-27432026	MsG0480019795.01.T01:intron	35.0%
	AACAATAGTCGATGCAACAA+TGG	-	Chr4:27432631-27432650	None:intergenic	35.0%
	AACCATTTCATACTGTTGCA+TGG	-	Chr4:27430738-27430757	None:intergenic	35.0%
	AAGCAGAAGGAGATCTATAA+AGG	+	Chr4:27432451-27432470	MsG0480019795.01.T01:CDS	35.0%
	AAGCATGTTGAATACTTGGA+AGG	-	Chr4:27429130-27429149	None:intergenic	35.0%
	ACAGAGAAGAAAGAAATGAC+TGG	+	Chr4:27430374-27430393	MsG0480019795.01.T01:intron	35.0%
	ACGTGAAGCAAAACTGTATT+AGG	+	Chr4:27429349-27429368	MsG0480019795.01.T01:intron	35.0%
	ACTTCATGTTCCAGTTGTTT+GGG	+	Chr4:27430898-27430917	MsG0480019795.01.T01:CDS	35.0%
	AGTTGAAGTTCTTCATGAGA+AGG	+	Chr4:27429066-27429085	MsG0480019795.01.T01:five_prime_UTR	35.0%
	ATTCAAGGACTACAGTGTAT+TGG	+	Chr4:27429916-27429935	MsG0480019795.01.T01:intron	35.0%
	CACGGTAAACCAAAACATTT+GGG	-	Chr4:27429288-27429307	None:intergenic	35.0%
	CCGGAGAAACAAAAATCTAA+GGG	-	Chr4:27428628-27428647	None:intergenic	35.0%
	CTAACTCATCCTTACAAAAC+CGG	-	Chr4:27430021-27430040	None:intergenic	35.0%
	CTTGCAATTGCTTTATCTTC+AGG	-	Chr4:27432388-27432407	None:intergenic	35.0%
	GAATTAGGATATGGAAAGGA+AGG	+	Chr4:27430458-27430477	MsG0480019795.01.T01:CDS	35.0%
	GCTGTCATTGTGTTGAAATT+TGG	-	Chr4:27430046-27430065	None:intergenic	35.0%
	GGACTACTCAATAATTCTTC+AGG	-	Chr4:27429028-27429047	None:intergenic	35.0%
	GTTTATGAAGTTGAGCTCAT+TGG	+	Chr4:27430521-27430540	MsG0480019795.01.T01:CDS	35.0%
	TAAACTAAATCTGCCATTGG+TGG	-	Chr4:27430506-27430525	None:intergenic	35.0%
	TAAAGAGTGAGACATTTCCA+TGG	+	Chr4:27432006-27432025	MsG0480019795.01.T01:intron	35.0%
	TAGTGGCTTAATGTGTAAGA+CGG	+	Chr4:27429746-27429765	MsG0480019795.01.T01:intron	35.0%
	TATCTACCATTGGAGTTCAA+TGG	+	Chr4:27431293-27431312	MsG0480019795.01.T01:intron	35.0%
	TATGGCAAGATATGCTTAGT+AGG	-	Chr4:27431737-27431756	None:intergenic	35.0%
	TCACGGTAAACCAAAACATT+TGG	-	Chr4:27429289-27429308	None:intergenic	35.0%
	TCAGGAAAATAAACTAGAGG+AGG	+	Chr4:27430712-27430731	MsG0480019795.01.T01:CDS	35.0%
	TCCGGAGAAACAAAAATCTA+AGG	-	Chr4:27428629-27428648	None:intergenic	35.0%
	TCTTGTGTCCATTGATCAAT+TGG	+	Chr4:27429615-27429634	MsG0480019795.01.T01:intron	35.0%
	TGAAAAGTTATAGTTGGCTG+AGG	-	Chr4:27432047-27432066	None:intergenic	35.0%
	TGATTATCTTGGTTCATGTC+AGG	+	Chr4:27430854-27430873	MsG0480019795.01.T01:intron	35.0%
	TGGTAGCAATTACAAGGTTA+GGG	-	Chr4:27432883-27432902	None:intergenic	35.0%
	TTACAATCCAAAGCTCACTT+TGG	-	Chr4:27431884-27431903	None:intergenic	35.0%
	TTCAAGGACTACAGTGTATT+GGG	+	Chr4:27429917-27429936	MsG0480019795.01.T01:intron	35.0%
	TTTAGTCCATTGAACTCCAA+TGG	-	Chr4:27431302-27431321	None:intergenic	35.0%
	TTTATGTCCTTGGAGTTCTA+AGG	-	Chr4:27431363-27431382	None:intergenic	35.0%
!	ACTTCAGAATCAACTTTTGG+AGG	-	Chr4:27429049-27429068	None:intergenic	35.0%
!	AGTCATTTGTTGAAACTTGC+AGG	+	Chr4:27432246-27432265	MsG0480019795.01.T01:intron	35.0%
!	ATGAACCAATTCCTTTTCTG+TGG	-	Chr4:27432505-27432524	None:intergenic	35.0%
!	TACAACATTTTGAGCTACTG+AGG	+	Chr4:27432675-27432694	MsG0480019795.01.T01:three_prime_UTR	35.0%
!	TCAAAGTCATGCTGTTTGAT+TGG	+	Chr4:27431416-27431435	MsG0480019795.01.T01:intron	35.0%
!	TTATGTCCTTGGAGTTCTAA+GGG	-	Chr4:27431362-27431381	None:intergenic	35.0%
!	TTGGTAGCAATTACAAGGTT+AGG	-	Chr4:27432884-27432903	None:intergenic	35.0%
!	TTTTCCAAATGTTCCACCAA+TGG	+	Chr4:27430490-27430509	MsG0480019795.01.T01:CDS	35.0%
!	TTTTTCACGGTTGAAAAGTG+TGG	-	Chr4:27432569-27432588	None:intergenic	35.0%
!!	AAGAAAGAAATGACTGGCTT+GGG	+	Chr4:27430380-27430399	MsG0480019795.01.T01:exon	35.0%
!!	AGCTTTTAGAAAATCTTCCC+TGG	-	Chr4:27432353-27432372	None:intergenic	35.0%
!!	CCCTTAGATTTTTGTTTCTC+CGG	+	Chr4:27428625-27428644	MsG0480019795.01.T01:intron	35.0%
!!	TTGAAGGCTTTATTTAGGAG+AGG	+	Chr4:27432292-27432311	MsG0480019795.01.T01:CDS	35.0%
!!!	AAATGTTTTGGTTTACCGTG+AGG	+	Chr4:27429288-27429307	MsG0480019795.01.T01:intron	35.0%
!!!	TTTTTTTTCCAGCAACATGG+AGG	+	Chr4:27428689-27428708	MsG0480019795.01.T01:intron	35.0%
	AAAGTTATAGTTGGCTGAGG+AGG	-	Chr4:27432044-27432063	None:intergenic	40.0%
	AACCGCTATGAAGAAGCTAT+AGG	+	Chr4:27430993-27431012	MsG0480019795.01.T01:CDS	40.0%
	ACCTTGTAATTGCTACCAAC+AGG	+	Chr4:27432885-27432904	MsG0480019795.01.T01:three_prime_UTR	40.0%
	ACTACCACCATAAACCATAC+AGG	-	Chr4:27429478-27429497	None:intergenic	40.0%
	AGAGCTCATAAGAAGAGTAG+AGG	-	Chr4:27429403-27429422	None:intergenic	40.0%
	AGTAGACTCAACCCAAAACT+CGG	-	Chr4:27431500-27431519	None:intergenic	40.0%
	ATGACCTGTATGGTTTATGG+TGG	+	Chr4:27429471-27429490	MsG0480019795.01.T01:intron	40.0%
	ATGTTGTAGCCTTTCATAGG+TGG	-	Chr4:27432663-27432682	None:intergenic	40.0%
	ATTTGAGGACCAAACTCACT+AGG	+	Chr4:27429855-27429874	MsG0480019795.01.T01:intron	40.0%
	CAGTACCACAGAAAAGGAAT+TGG	+	Chr4:27432497-27432516	MsG0480019795.01.T01:CDS	40.0%
	CATATTTGTTTGGCAGTGGT+TGG	+	Chr4:27432522-27432541	MsG0480019795.01.T01:CDS	40.0%
	CGACCAATTGAGATGGTAAT+AGG	+	Chr4:27429559-27429578	MsG0480019795.01.T01:intron	40.0%
	CTGGGAATTAGGATATGGAA+AGG	+	Chr4:27430454-27430473	MsG0480019795.01.T01:CDS	40.0%
	CTGTCTAAGAATGCTTTCAC+TGG	-	Chr4:27431698-27431717	None:intergenic	40.0%
	GAAACTTACGTTGACCAGAT+CGG	-	Chr4:27428580-27428599	None:intergenic	40.0%
	GAATCCATGCCATCTTAACA+CGG	+	Chr4:27430950-27430969	MsG0480019795.01.T01:CDS	40.0%
	GACGCATTGAAGGACAAAAA+TGG	+	Chr4:27430070-27430089	MsG0480019795.01.T01:intron	40.0%
	GACTTCATGTTCCAGTTGTT+TGG	+	Chr4:27430897-27430916	MsG0480019795.01.T01:CDS	40.0%
	GGACTCAACAGTAGTATCAA+TGG	-	Chr4:27428534-27428553	None:intergenic	40.0%
	GGATGGAAATGTTCTGTTTC+AGG	+	Chr4:27430694-27430713	MsG0480019795.01.T01:CDS	40.0%
	GGTTCATATTTGTTTGGCAG+TGG	+	Chr4:27432518-27432537	MsG0480019795.01.T01:CDS	40.0%
	GTCCTATAGCTTCTTCATAG+CGG	-	Chr4:27430998-27431017	None:intergenic	40.0%
	GTGATATGACTGTAGAGGAA+CGG	+	Chr4:27430645-27430664	MsG0480019795.01.T01:CDS	40.0%
	TATGGTGGTAGTCCATTACA+GGG	+	Chr4:27429486-27429505	MsG0480019795.01.T01:intron	40.0%
	TATGTGCATCCACCTATGAA+AGG	+	Chr4:27432651-27432670	MsG0480019795.01.T01:three_prime_UTR	40.0%
	TCACTCAGCATTTATGTCCT+TGG	-	Chr4:27431373-27431392	None:intergenic	40.0%
	TCTAAGGGAGAGAGAAATTG+AGG	-	Chr4:27428613-27428632	None:intergenic	40.0%
	TCTCAACCACAATCAAAACC+AGG	+	Chr4:27428723-27428742	MsG0480019795.01.T01:intron	40.0%
	TGAGGATGAGAGCAATTTGA+AGG	+	Chr4:27432276-27432295	MsG0480019795.01.T01:CDS	40.0%
	TGTCAAACACGATTACTCTG+TGG	-	Chr4:27432771-27432790	None:intergenic	40.0%
	TTATGACATCTAGCGTAGTG+TGG	+	Chr4:27430271-27430290	MsG0480019795.01.T01:intron	40.0%
	TTATGGTGGTAGTCCATTAC+AGG	+	Chr4:27429485-27429504	MsG0480019795.01.T01:intron	40.0%
	TTTGTCCTTCAATGCGTCAA+GGG	-	Chr4:27430068-27430087	None:intergenic	40.0%
!	AATGAGTTCTGGCAAATGAC+AGG	+	Chr4:27430179-27430198	MsG0480019795.01.T01:intron	40.0%
!	ATGAGTTCTGGCAAATGACA+GGG	+	Chr4:27430180-27430199	MsG0480019795.01.T01:intron	40.0%
!	ATTGAGTTCTGGCAAATGAC+AGG	+	Chr4:27430151-27430170	MsG0480019795.01.T01:intron	40.0%
!	CAATTCCTTTTCTGTGGTAC+TGG	-	Chr4:27432499-27432518	None:intergenic	40.0%
!	CCAGTTGTTTGGGAAGTATA+GGG	+	Chr4:27430908-27430927	MsG0480019795.01.T01:CDS	40.0%
!	GAAGAAAGAAATGACTGGCT+TGG	+	Chr4:27430379-27430398	MsG0480019795.01.T01:exon	40.0%
!	GCATCTTTGATCAGAAGTGT+TGG	+	Chr4:27428880-27428899	MsG0480019795.01.T01:intron	40.0%
!	GTTTGGGAAGTATAGGGATA+TGG	+	Chr4:27430914-27430933	MsG0480019795.01.T01:CDS	40.0%
!	TCCAGTTGTTTGGGAAGTAT+AGG	+	Chr4:27430907-27430926	MsG0480019795.01.T01:CDS	40.0%
!	TGTCATTTGCCAGAACTCAA+TGG	-	Chr4:27430152-27430171	None:intergenic	40.0%
!	TGTCATTTGCCAGAACTCAT+TGG	-	Chr4:27430180-27430199	None:intergenic	40.0%
!	TTGAGTTCTGGCAAATGACA+GGG	+	Chr4:27430152-27430171	MsG0480019795.01.T01:intron	40.0%
!	TTTTGTCCTTCAATGCGTCA+AGG	-	Chr4:27430069-27430088	None:intergenic	40.0%
!!	GGCTTTATTTAGGAGAGGTA+AGG	+	Chr4:27432297-27432316	MsG0480019795.01.T01:CDS	40.0%
!!!	AGGTCTTTCATCGGTTTTGA+TGG	-	Chr4:27431212-27431231	None:intergenic	40.0%
	AGAGTCTCATCTATCCGATG+TGG	-	Chr4:27429956-27429975	None:intergenic	45.0%
	AGCATGAGCGCACAATATTG+AGG	+	Chr4:27431548-27431567	MsG0480019795.01.T01:intron	45.0%
	ATCAAAGATGCGTCTGGTGT+CGG	-	Chr4:27428871-27428890	None:intergenic	45.0%
	ATCTATCCGATGTGGGACTT+GGG	-	Chr4:27429948-27429967	None:intergenic	45.0%
	ATGACTGTAGAGGAACGGAT+TGG	+	Chr4:27430650-27430669	MsG0480019795.01.T01:CDS	45.0%
	ATGTCAGGCCATAGCTTACA+TGG	+	Chr4:27430869-27430888	MsG0480019795.01.T01:intron	45.0%
	CACACGAACAAAAACCGATC+TGG	+	Chr4:27428563-27428582	MsG0480019795.01.T01:five_prime_UTR	45.0%
	CATGTCAGTTACATGCCTGT+TGG	-	Chr4:27432903-27432922	None:intergenic	45.0%
	CCCTATACTTCCCAAACAAC+TGG	-	Chr4:27430911-27430930	None:intergenic	45.0%
	CTGAACCAGTACCACAGAAA+AGG	+	Chr4:27432491-27432510	MsG0480019795.01.T01:CDS	45.0%
	CTTCTGATCAAAGATGCGTC+TGG	-	Chr4:27428877-27428896	None:intergenic	45.0%
	GAAAAACCCAAGTCCCACAT+CGG	+	Chr4:27429939-27429958	MsG0480019795.01.T01:intron	45.0%
	GAAGTATAGGGATATGGCTC+TGG	+	Chr4:27430920-27430939	MsG0480019795.01.T01:CDS	45.0%
	GAGTCTCATCTATCCGATGT+GGG	-	Chr4:27429955-27429974	None:intergenic	45.0%
	GATCGAGGGACGAAATTGAT+TGG	-	Chr4:27431167-27431186	None:intergenic	45.0%
	GCATCTTGTCCTAGTGAGTT+TGG	-	Chr4:27429867-27429886	None:intergenic	45.0%
	GCATTCTTAGACAGAAGCTG+TGG	+	Chr4:27431704-27431723	MsG0480019795.01.T01:intron	45.0%
	GGAGGTAATTTCTACTCCCA+TGG	-	Chr4:27432026-27432045	None:intergenic	45.0%
	GGCCATGCAACAGTATGAAA+TGG	+	Chr4:27430733-27430752	MsG0480019795.01.T01:CDS	45.0%
	GTATCCATGCCATTGAGTTC+TGG	+	Chr4:27430140-27430159	MsG0480019795.01.T01:intron	45.0%
	GTCATCTCCCATGTAAGCTA+TGG	-	Chr4:27430880-27430899	None:intergenic	45.0%
	GTGAGCTGGGAATTAGGATA+TGG	+	Chr4:27430449-27430468	MsG0480019795.01.T01:CDS	45.0%
	TCGCAGTGATATGACTGTAG+AGG	+	Chr4:27430640-27430659	MsG0480019795.01.T01:CDS	45.0%
	TCTGCCATTGGTGGAACATT+TGG	-	Chr4:27430497-27430516	None:intergenic	45.0%
	TGTCAGGCCATAGCTTACAT+GGG	+	Chr4:27430870-27430889	MsG0480019795.01.T01:intron	45.0%
	TGTTGCGTCAGTGTTGTATC+TGG	-	Chr4:27428787-27428806	None:intergenic	45.0%
	TTGAACTTCCTCCATGTTGC+TGG	-	Chr4:27428700-27428719	None:intergenic	45.0%
	TTTGCCAGAACTCAATGGCA+TGG	-	Chr4:27430147-27430166	None:intergenic	45.0%
	TTTGTTCGTGTGTGGTGTGT+GGG	-	Chr4:27428555-27428574	None:intergenic	45.0%
!	TGACTGTAGAGGAACGGATT+GGG	+	Chr4:27430651-27430670	MsG0480019795.01.T01:CDS	45.0%
!	TGCTCACACGTTTTGTCGTA+AGG	+	Chr4:27431603-27431622	MsG0480019795.01.T01:intron	45.0%
!	TTTTGTTCGTGTGTGGTGTG+TGG	-	Chr4:27428556-27428575	None:intergenic	45.0%
!!	AAATGACTGGCTTGGGCATT+GGG	+	Chr4:27430387-27430406	MsG0480019795.01.T01:exon	45.0%
!!	CCTTACAAAACCGGCTTGTA+AGG	-	Chr4:27430012-27430031	None:intergenic	45.0%
!!	CCTTACAAGCCGGTTTTGTA+AGG	+	Chr4:27430009-27430028	MsG0480019795.01.T01:intron	45.0%
!!	GAAGTGTTGGTGTTACAGAG+AGG	+	Chr4:27428893-27428912	MsG0480019795.01.T01:intron	45.0%
!!	GATCGGTTTTTGTTCGTGTG+TGG	-	Chr4:27428563-27428582	None:intergenic	45.0%
!!	GCCTGTTGGTAGCAATTACA+AGG	-	Chr4:27432889-27432908	None:intergenic	45.0%
!!!	CTTTTCTGTGGTACTGGTTC+AGG	-	Chr4:27432493-27432512	None:intergenic	45.0%
	AGCGAGAGTAACAACAGCGA+GGG	-	Chr4:27428500-27428519	None:intergenic	50.0%
	CATCTATCCGATGTGGGACT+TGG	-	Chr4:27429949-27429968	None:intergenic	50.0%
	GAATTATATCCCTGGGACCC+AGG	-	Chr4:27431955-27431974	None:intergenic	50.0%
	GCAGATCGGAGAAAGATGGA+TGG	+	Chr4:27430677-27430696	MsG0480019795.01.T01:CDS	50.0%
	GCGGTTCAGCTTAATCAGAC+AGG	-	Chr4:27430979-27430998	None:intergenic	50.0%
	GGAGCAACGACCAATTGAGA+TGG	+	Chr4:27429552-27429571	MsG0480019795.01.T01:intron	50.0%
	GTAAGGCTAGAGCAGCACTT+GGG	+	Chr4:27432314-27432333	MsG0480019795.01.T01:CDS	50.0%
	TACCGACCCTTAGAACTCCA+AGG	+	Chr4:27431353-27431372	MsG0480019795.01.T01:intron	50.0%
	TAGCGAGAGTAACAACAGCG+AGG	-	Chr4:27428501-27428520	None:intergenic	50.0%
	TCCATGCCATCTTAACACGG+CGG	+	Chr4:27430953-27430972	MsG0480019795.01.T01:CDS	50.0%
!!	AATGACTGGCTTGGGCATTG+GGG	+	Chr4:27430388-27430407	MsG0480019795.01.T01:exon	50.0%
!!	CAAAACCGGCTTGTAAGGTG+AGG	-	Chr4:27430007-27430026	None:intergenic	50.0%
!!	GAAATGACTGGCTTGGGCAT+TGG	+	Chr4:27430386-27430405	MsG0480019795.01.T01:exon	50.0%
!!	GACTGTAGAGGAACGGATTG+GGG	+	Chr4:27430652-27430671	MsG0480019795.01.T01:CDS	50.0%
!!	GTCCTTGGAGTTCTAAGGGT+CGG	-	Chr4:27431358-27431377	None:intergenic	50.0%
	CTGCGTGTGAGCTGGGAATT+AGG	+	Chr4:27430443-27430462	MsG0480019795.01.T01:CDS	55.0%
	GACAGCCCTTGACGCATTGA+AGG	+	Chr4:27430060-27430079	MsG0480019795.01.T01:intron	55.0%
	GCACTCCTCACCTTACAAGC+CGG	+	Chr4:27429999-27430018	MsG0480019795.01.T01:intron	55.0%
	GGCAGCAGATCGGAGAAAGA+TGG	+	Chr4:27430673-27430692	MsG0480019795.01.T01:CDS	55.0%
	GGGTGGCAAGCATGAAAGCT+GGG	+	Chr4:27430408-27430427	MsG0480019795.01.T01:CDS	55.0%
	GGTAAGGCTAGAGCAGCACT+TGG	+	Chr4:27432313-27432332	MsG0480019795.01.T01:CDS	55.0%
	GGTGGCAAGCATGAAAGCTG+GGG	+	Chr4:27430409-27430428	MsG0480019795.01.T01:CDS	55.0%
	TCTCAGACCACGCCCTGTAA+TGG	-	Chr4:27429501-27429520	None:intergenic	55.0%
	TGGGCAGACAGATGTTGCCA+GGG	+	Chr4:27432333-27432352	MsG0480019795.01.T01:CDS	55.0%
	TGGTAGTCCATTACAGGGCG+TGG	+	Chr4:27429491-27429510	MsG0480019795.01.T01:intron	55.0%
	TTGGGCAGACAGATGTTGCC+AGG	+	Chr4:27432332-27432351	MsG0480019795.01.T01:CDS	55.0%
!	GACAGGGTGCCAATGAGTTC+TGG	+	Chr4:27430168-27430187	MsG0480019795.01.T01:intron	55.0%
!!	AACGGATTGGGGCAGCAGAT+CGG	+	Chr4:27430663-27430682	MsG0480019795.01.T01:CDS	55.0%
	AGCGTTGCTGCGTGTGAGCT+GGG	+	Chr4:27430436-27430455	MsG0480019795.01.T01:CDS	60.0%
	CAGGGCGTGGTCTGAGAACA+CGG	+	Chr4:27429504-27429523	MsG0480019795.01.T01:intron	60.0%
	CTGCGATTTCCTGGGTCCCA+GGG	+	Chr4:27431943-27431962	MsG0480019795.01.T01:intron	60.0%
	GACAGGCCGCCGTGTTAAGA+TGG	-	Chr4:27430962-27430981	None:intergenic	60.0%
	GCCGCCGTGTTAAGATGGCA+TGG	-	Chr4:27430957-27430976	None:intergenic	60.0%
	GGGGTGGCAAGCATGAAAGC+TGG	+	Chr4:27430407-27430426	MsG0480019795.01.T01:CDS	60.0%
	TCTGCGATTTCCTGGGTCCC+AGG	+	Chr4:27431942-27431961	MsG0480019795.01.T01:intron	60.0%
!	GAGCGTTGCTGCGTGTGAGC+TGG	+	Chr4:27430435-27430454	MsG0480019795.01.T01:CDS	65.0%
!!	GACTGGCTTGGGCATTGGGG+TGG	+	Chr4:27430391-27430410	MsG0480019795.01.T01:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	27428485	27432959	27428485	ID=MsG0480019795.01;Name=MsG0480019795.01
Chr4	mRNA	27428485	27432959	27428485	ID=MsG0480019795.01.T01;Parent=MsG0480019795.01;Name=MsG0480019795.01.T01;_AED=0.39;_eAED=0.41;_QI=306\|0.6\|0.83\|1\|1\|1\|6\|353\|262
Chr4	exon	27428485	27428590	27428485	ID=MsG0480019795.01.T01:exon:16412;Parent=MsG0480019795.01.T01
Chr4	exon	27428963	27429151	27428963	ID=MsG0480019795.01.T01:exon:16413;Parent=MsG0480019795.01.T01
Chr4	exon	27430378	27430562	27430378	ID=MsG0480019795.01.T01:exon:16414;Parent=MsG0480019795.01.T01
Chr4	exon	27430632	27430754	27430632	ID=MsG0480019795.01.T01:exon:16415;Parent=MsG0480019795.01.T01
Chr4	exon	27430876	27431028	27430876	ID=MsG0480019795.01.T01:exon:16416;Parent=MsG0480019795.01.T01
Chr4	exon	27432268	27432959	27432268	ID=MsG0480019795.01.T01:exon:16417;Parent=MsG0480019795.01.T01
Chr4	five_prime_UTR	27428485	27428590	27428485	ID=MsG0480019795.01.T01:five_prime_utr;Parent=MsG0480019795.01.T01
Chr4	five_prime_UTR	27428963	27429151	27428963	ID=MsG0480019795.01.T01:five_prime_utr;Parent=MsG0480019795.01.T01
Chr4	five_prime_UTR	27430378	27430388	27430378	ID=MsG0480019795.01.T01:five_prime_utr;Parent=MsG0480019795.01.T01
Chr4	CDS	27430389	27430562	27430389	ID=MsG0480019795.01.T01:cds;Parent=MsG0480019795.01.T01
Chr4	CDS	27430632	27430754	27430632	ID=MsG0480019795.01.T01:cds;Parent=MsG0480019795.01.T01
Chr4	CDS	27430876	27431028	27430876	ID=MsG0480019795.01.T01:cds;Parent=MsG0480019795.01.T01
Chr4	CDS	27432268	27432606	27432268	ID=MsG0480019795.01.T01:cds;Parent=MsG0480019795.01.T01
Chr4	three_prime_UTR	27432607	27432959	27432607	ID=MsG0480019795.01.T01:three_prime_utr;Parent=MsG0480019795.01.T01

Gene Sequence

>MsG0480019795.01.T01

ATGACTGGCTTGGGCATTGGGGTGGCAAGCATGAAAGCTGGGGAACGAGCGTTGCTGCGTGTGAGCTGGGAATTAGGATATGGAAAGGAAGGAAGCTTTTCTTTTCCAAATGTTCCACCAATGGCAGATTTAGTTTATGAAGTTGAGCTCATTGGATTTGATGAAACAAAAGATGGAAAAGCTCGCAGTGATATGACTGTAGAGGAACGGATTGGGGCAGCAGATCGGAGAAAGATGGATGGAAATGTTCTGTTTCAGGAAAATAAACTAGAGGAGGCCATGCAACAGTATGAAATGGCCATAGCTTACATGGGAGATGACTTCATGTTCCAGTTGTTTGGGAAGTATAGGGATATGGCTCTGGCTGTAAAGAATCCATGCCATCTTAACACGGCGGCCTGTCTGATTAAGCTGAACCGCTATGAAGAAGCTATAGGACAATGCAGCATTGTATTAAGTGAGGATGAGAGCAATTTGAAGGCTTTATTTAGGAGAGGTAAGGCTAGAGCAGCACTTGGGCAGACAGATGTTGCCAGGGAAGATTTTCTAAAAGCTCGTAAGCATGCACCTGAAGATAAAGCAATTGCAAGAGAATTGAAGCTACTTGCTGAACATGATAAAGCTATTTATCAAAAGCAGAAGGAGATCTATAAAGGAATATTTGGACCAAGACCTGAACCAGTACCACAGAAAAGGAATTGGTTCATATTTGTTTGGCAGTGGTTGGTTTCAGTGTTTCATAGCTTTACCACACTTTTCAACCGTGAAAAAAAGCACAAAGATGACTAA

Protein sequence

>MsG0480019795.01.T01

MTGLGIGVASMKAGERALLRVSWELGYGKEGSFSFPNVPPMADLVYEVELIGFDETKDGKARSDMTVEERIGAADRRKMDGNVLFQENKLEEAMQQYEMAIAYMGDDFMFQLFGKYRDMALAVKNPCHLNTAACLIKLNRYEEAIGQCSIVLSEDESNLKALFRRGKARAALGQTDVAREDFLKARKHAPEDKAIARELKLLAEHDKAIYQKQKEIYKGIFGPRPEPVPQKRNWFIFVWQWLVSVFHSFTTLFNREKKHKDD*