Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009673.01.T01 | AES66446.1 | 100 | 72 | 0 | 0 | 1 | 72 | 1 | 72 | 6.56E-45 | 148 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009673.01.T01 | G7IH42 | 100.000 | 72 | 0 | 0 | 1 | 72 | 1 | 72 | 3.13e-45 | 148 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004328.01 | MsG0280009673.01 | 0.844472 | 7.401641e-59 | 1.196214e-55 |
| MsG0280009673.01 | MsG0280010005.01 | 0.811538 | 6.658963e-51 | 4.132899e-48 |
| MsG0280009673.01 | MsG0280010417.01 | 0.870107 | 1.872891e-66 | 7.198953e-63 |
| MsG0280009673.01 | MsG0380012254.01 | 0.811525 | 6.702627e-51 | 4.158628e-48 |
| MsG0280009673.01 | MsG0380013101.01 | 0.804657 | 1.944536e-49 | 1.008280e-46 |
| MsG0280009673.01 | MsG0380016481.01 | 0.817125 | 3.877394e-52 | 2.799572e-49 |
| MsG0280009673.01 | MsG0380016853.01 | 0.842681 | 2.228311e-58 | 3.404279e-55 |
| MsG0280009673.01 | MsG0380017151.01 | 0.806614 | 7.551054e-50 | 4.116898e-47 |
| MsG0280009673.01 | MsG0480018522.01 | 0.803894 | 2.803787e-49 | 1.425658e-46 |
| MsG0280009673.01 | MsG0480022303.01 | 0.815090 | 1.104484e-51 | 7.542322e-49 |
| MsG0280009673.01 | MsG0480022638.01 | 0.827945 | 1.181632e-54 | 1.157742e-51 |
| MsG0280009673.01 | MsG0480023102.01 | 0.824602 | 7.386120e-54 | 6.574052e-51 |
| MsG0280009673.01 | MsG0480023349.01 | 0.804722 | 1.884278e-49 | 9.787648e-47 |
| MsG0280009673.01 | MsG0480023693.01 | 0.835942 | 1.250879e-56 | 1.552866e-53 |
| MsG0280009673.01 | MsG0580024202.01 | 0.802612 | 5.166068e-49 | 2.541829e-46 |
| MsG0280009673.01 | MsG0780038963.01 | 0.804834 | 1.786114e-49 | 9.304349e-47 |
| MsG0280009673.01 | MsG0780039665.01 | 0.813391 | 2.620779e-51 | 1.709391e-48 |
| MsG0280009673.01 | MsG0880042877.01 | 0.804040 | 2.614474e-49 | 1.334350e-46 |
| MsG0280009673.01 | MsG0880046768.01 | 0.809960 | 1.461678e-50 | 8.701337e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009673.01.T01 | MTR_2g069400 | 100.000 | 72 | 0 | 0 | 1 | 72 | 1 | 72 | 7.94e-49 | 148 |
| MsG0280009673.01.T01 | MTR_2g069430 | 60.526 | 76 | 26 | 1 | 1 | 72 | 1 | 76 | 2.17e-18 | 71.6 |
| MsG0280009673.01.T01 | MTR_2g069420 | 58.228 | 79 | 25 | 3 | 1 | 72 | 1 | 78 | 2.96e-15 | 63.9 |
| MsG0280009673.01.T01 | MTR_4g091090 | 61.842 | 76 | 22 | 3 | 4 | 72 | 5 | 80 | 3.11e-15 | 63.9 |
| MsG0280009673.01.T01 | MTR_1g040735 | 53.333 | 75 | 26 | 3 | 6 | 72 | 63 | 136 | 3.18e-13 | 60.1 |
| MsG0280009673.01.T01 | MTR_5g042360 | 51.351 | 74 | 29 | 2 | 6 | 72 | 6 | 79 | 1.77e-12 | 57.0 |
| MsG0280009673.01.T01 | MTR_1g040775 | 51.351 | 74 | 29 | 2 | 6 | 72 | 6 | 79 | 1.77e-12 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009673.01.T01 | AT2G32190 | 45.205 | 73 | 37 | 2 | 1 | 72 | 1 | 71 | 1.42e-14 | 62.0 |
| MsG0280009673.01.T01 | AT2G32210 | 48.333 | 60 | 30 | 1 | 14 | 72 | 12 | 71 | 2.48e-14 | 61.6 |
| MsG0280009673.01.T01 | AT2G32200 | 44.444 | 63 | 31 | 2 | 14 | 72 | 46 | 108 | 1.28e-11 | 55.5 |
| MsG0280009673.01.T01 | AT2G32200 | 44.444 | 63 | 31 | 2 | 14 | 72 | 12 | 74 | 2.32e-11 | 53.9 |
Find 42 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTAGCAGGGGTGATGGTTTC+TGG | 0.154253 | 2:+56049898 | MsG0280009673.01.T01:CDS |
| AATATTTGTGCTTGTCTTCA+TGG | 0.210203 | 2:+56050432 | MsG0280009673.01.T01:three_prime_UTR |
| TAAAGATCCATGTTACAAAT+TGG | 0.278218 | 2:-56050536 | None:intergenic |
| AGGATACAAAGATTCTTATT+AGG | 0.301506 | 2:-56050492 | None:intergenic |
| GATAGAATATATGTATAAAT+AGG | 0.313006 | 2:-56049423 | None:intergenic |
| TCCAAGTGTGTGTGATTAAC+TGG | 0.369868 | 2:-56049461 | None:intergenic |
| TTTAATTGGAACACTTTGTT+GGG | 0.371717 | 2:-56049871 | None:intergenic |
| ATAAGAATCTTTGTATCCTA+TGG | 0.390499 | 2:+56050496 | MsG0280009673.01.T01:three_prime_UTR |
| AGCTGTTCCACCACCACCTA+TGG | 0.399384 | 2:+56049827 | MsG0280009673.01.T01:CDS |
| ATTATGTTGTTGCTGTGCCC+TGG | 0.400888 | 2:+56050368 | MsG0280009673.01.T01:CDS |
| ATATTGTGACTCATTGTGTT+AGG | 0.426555 | 2:-56049546 | None:intergenic |
| ACTTTGTTGGGGATCATCCT+TGG | 0.445500 | 2:-56049859 | None:intergenic |
| CTGATCGAATATCATACAAC+TGG | 0.456561 | 2:+56050401 | MsG0280009673.01.T01:exon |
| TCGATCAGAGGCAACAATCC+AGG | 0.459282 | 2:-56050386 | None:intergenic |
| TTGTTGGGGATCATCCTTGG+AGG | 0.481114 | 2:-56049856 | None:intergenic |
| GGGTTGTTCTGACTCACTGC+TGG | 0.483166 | 2:-56049801 | None:intergenic |
| TTCCAATTAAAACAACTAGC+AGG | 0.486226 | 2:+56049883 | MsG0280009673.01.T01:CDS |
| GGATAACCCATAGGTGGTGG+TGG | 0.506331 | 2:-56049834 | None:intergenic |
| AAAACAACTAGCAGGGGTGA+TGG | 0.528737 | 2:+56049891 | MsG0280009673.01.T01:CDS |
| ATCAATCAACAACAATCTCC+TGG | 0.529469 | 2:+56049567 | MsG0280009673.01.T01:CDS |
| TAAGAATCTTTGTATCCTAT+GGG | 0.536702 | 2:+56050497 | MsG0280009673.01.T01:three_prime_UTR |
| GGTGGTGGTGGAACAGCTTG+AGG | 0.543912 | 2:-56049822 | None:intergenic |
| CGATCAGAGGCAACAATCCA+GGG | 0.546345 | 2:-56050385 | None:intergenic |
| TCCAGTTAATCACACACACT+TGG | 0.546579 | 2:+56049460 | MsG0280009673.01.T01:five_prime_UTR |
| GTGGTGGTGGAACAGCTTGA+GGG | 0.555087 | 2:-56049821 | None:intergenic |
| GAGGGATAACCCATAGGTGG+TGG | 0.556470 | 2:-56049837 | None:intergenic |
| CAAAGATTCTTATTAGGTGA+GGG | 0.558800 | 2:-56050486 | None:intergenic |
| TCCAATTAAAACAACTAGCA+GGG | 0.562056 | 2:+56049884 | MsG0280009673.01.T01:CDS |
| ACAAAGATTCTTATTAGGTG+AGG | 0.569641 | 2:-56050487 | None:intergenic |
| AGGGGTGATGGTTTCTGGAA+AGG | 0.573043 | 2:+56049903 | MsG0280009673.01.T01:CDS |
| ATTCATACCAATTTGTAACA+TGG | 0.575973 | 2:+56050529 | MsG0280009673.01.T01:three_prime_UTR |
| GCTGTTCCACCACCACCTAT+GGG | 0.592890 | 2:+56049828 | MsG0280009673.01.T01:CDS |
| ACCTATGGGTTATCCCTCCA+AGG | 0.593969 | 2:+56049842 | MsG0280009673.01.T01:CDS |
| GTGATGGTTTCTGGAAAGGA+TGG | 0.594474 | 2:+56049907 | MsG0280009673.01.T01:CDS |
| GTTGTATGATATTCGATCAG+AGG | 0.597281 | 2:-56050398 | None:intergenic |
| TGTTGGGGATCATCCTTGGA+GGG | 0.597882 | 2:-56049855 | None:intergenic |
| TTAATTGGAACACTTTGTTG+GGG | 0.602125 | 2:-56049870 | None:intergenic |
| CCAATTAAAACAACTAGCAG+GGG | 0.643519 | 2:+56049885 | MsG0280009673.01.T01:CDS |
| TCCTTGGAGGGATAACCCAT+AGG | 0.667495 | 2:-56049843 | None:intergenic |
| TAACTGGACGATGAGAAGAA+CGG | 0.674180 | 2:-56049445 | None:intergenic |
| TTGGAGGGATAACCCATAGG+TGG | 0.675232 | 2:-56049840 | None:intergenic |
| TTGCAACGACACAAGTACAA+AGG | 0.678074 | 2:-56050563 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATAGTTATAGTTATTAATT+AGG | - | Chr2:56049951-56049970 | None:intergenic | 10.0% |
| !! | ATAATTAAAAAAAACTTACC+AGG | - | Chr2:56049588-56049607 | None:intergenic | 15.0% |
| !! | CATTTCTATAAGAAATAATT+TGG | + | Chr2:56049724-56049743 | MsG0280009673.01.T01:intron | 15.0% |
| !! | GATAGAATATATGTATAAAT+AGG | - | Chr2:56049426-56049445 | None:intergenic | 15.0% |
| !! | CAAAAAAATTAAACGATTCT+AGG | - | Chr2:56049690-56049709 | None:intergenic | 20.0% |
| !! | CAACTGCAAAAAAAAAAAAA+AGG | - | Chr2:56050342-56050361 | None:intergenic | 20.0% |
| !! | TATTTATGCTAAAAACAGTT+TGG | - | Chr2:56050299-56050318 | None:intergenic | 20.0% |
| !!! | TGCGATAACTTTTTTTTTAA+TGG | - | Chr2:56050154-56050173 | None:intergenic | 20.0% |
| ! | AGGATACAAAGATTCTTATT+AGG | - | Chr2:56050495-56050514 | None:intergenic | 25.0% |
| ! | ATAAGAATCTTTGTATCCTA+TGG | + | Chr2:56050496-56050515 | MsG0280009673.01.T01:three_prime_UTR | 25.0% |
| ! | ATTCATACCAATTTGTAACA+TGG | + | Chr2:56050529-56050548 | MsG0280009673.01.T01:three_prime_UTR | 25.0% |
| ! | TAAAGATCCATGTTACAAAT+TGG | - | Chr2:56050539-56050558 | None:intergenic | 25.0% |
| ! | TAAGAATCTTTGTATCCTAT+GGG | + | Chr2:56050497-56050516 | MsG0280009673.01.T01:three_prime_UTR | 25.0% |
| ! | TTTAATTGGAACACTTTGTT+GGG | - | Chr2:56049874-56049893 | None:intergenic | 25.0% |
| !! | TAACTTCTTTTCCTTTAACT+CGG | - | Chr2:56050064-56050083 | None:intergenic | 25.0% |
| !!! | AGTTTTTTTCTCCTTTGTTA+GGG | - | Chr2:56050104-56050123 | None:intergenic | 25.0% |
| !!! | TAGTTTTTTTCTCCTTTGTT+AGG | - | Chr2:56050105-56050124 | None:intergenic | 25.0% |
| !!! | TTTTAATTGGAACACTTTGT+TGG | - | Chr2:56049875-56049894 | None:intergenic | 25.0% |
| AATATTTGTGCTTGTCTTCA+TGG | + | Chr2:56050432-56050451 | MsG0280009673.01.T01:three_prime_UTR | 30.0% | |
| ACAAAGATTCTTATTAGGTG+AGG | - | Chr2:56050490-56050509 | None:intergenic | 30.0% | |
| ATATTGTGACTCATTGTGTT+AGG | - | Chr2:56049549-56049568 | None:intergenic | 30.0% | |
| CAAAGATTCTTATTAGGTGA+GGG | - | Chr2:56050489-56050508 | None:intergenic | 30.0% | |
| CTAGGCTTGAATATAAAACA+TGG | - | Chr2:56049672-56049691 | None:intergenic | 30.0% | |
| GAAATTGAAAACCGAGTTAA+AGG | + | Chr2:56050050-56050069 | MsG0280009673.01.T01:intron | 30.0% | |
| GTTAAAGGAAAAGAAGTTAG+AGG | + | Chr2:56050065-56050084 | MsG0280009673.01.T01:intron | 30.0% | |
| TCCAATTAAAACAACTAGCA+GGG | + | Chr2:56049884-56049903 | MsG0280009673.01.T01:CDS | 30.0% | |
| TTAATTGGAACACTTTGTTG+GGG | - | Chr2:56049873-56049892 | None:intergenic | 30.0% | |
| TTCCAATTAAAACAACTAGC+AGG | + | Chr2:56049883-56049902 | MsG0280009673.01.T01:CDS | 30.0% | |
| !!! | TTTTTTTTTTGCAGTTGTGC+TGG | + | Chr2:56050345-56050364 | MsG0280009673.01.T01:intron | 30.0% |
| ATCAATCAACAACAATCTCC+TGG | + | Chr2:56049567-56049586 | MsG0280009673.01.T01:CDS | 35.0% | |
| CCAATTAAAACAACTAGCAG+GGG | + | Chr2:56049885-56049904 | MsG0280009673.01.T01:CDS | 35.0% | |
| CGAAATACACAACACAAGTT+AGG | - | Chr2:56049762-56049781 | None:intergenic | 35.0% | |
| CTGATCGAATATCATACAAC+TGG | + | Chr2:56050401-56050420 | MsG0280009673.01.T01:exon | 35.0% | |
| GTTGTATGATATTCGATCAG+AGG | - | Chr2:56050401-56050420 | None:intergenic | 35.0% | |
| TTAGGTTCAAACCCTAACAA+AGG | + | Chr2:56050090-56050109 | MsG0280009673.01.T01:intron | 35.0% | |
| !! | GAAAAGAAGTTAGAGGTGTT+AGG | + | Chr2:56050072-56050091 | MsG0280009673.01.T01:intron | 35.0% |
| TAACTGGACGATGAGAAGAA+CGG | - | Chr2:56049448-56049467 | None:intergenic | 40.0% | |
| TCCAAGTGTGTGTGATTAAC+TGG | - | Chr2:56049464-56049483 | None:intergenic | 40.0% | |
| TCCAGTTAATCACACACACT+TGG | + | Chr2:56049460-56049479 | MsG0280009673.01.T01:five_prime_UTR | 40.0% | |
| TTGCAACGACACAAGTACAA+AGG | - | Chr2:56050566-56050585 | None:intergenic | 40.0% | |
| ! | ATGAATTTTCGCTGTCCCAT+AGG | - | Chr2:56050515-56050534 | None:intergenic | 40.0% |
| !!! | CCCCTGCTAGTTGTTTTAAT+TGG | - | Chr2:56049888-56049907 | None:intergenic | 40.0% |
| AAAACAACTAGCAGGGGTGA+TGG | + | Chr2:56049891-56049910 | MsG0280009673.01.T01:CDS | 45.0% | |
| ACTTTGTTGGGGATCATCCT+TGG | - | Chr2:56049862-56049881 | None:intergenic | 45.0% | |
| ATTATGTTGTTGCTGTGCCC+TGG | + | Chr2:56050368-56050387 | MsG0280009673.01.T01:CDS | 45.0% | |
| ! | GTGATGGTTTCTGGAAAGGA+TGG | + | Chr2:56049907-56049926 | MsG0280009673.01.T01:CDS | 45.0% |
| CGATCAGAGGCAACAATCCA+GGG | - | Chr2:56050388-56050407 | None:intergenic | 50.0% | |
| TCCTTGGAGGGATAACCCAT+AGG | - | Chr2:56049846-56049865 | None:intergenic | 50.0% | |
| TCGATCAGAGGCAACAATCC+AGG | - | Chr2:56050389-56050408 | None:intergenic | 50.0% | |
| TGTTGGGGATCATCCTTGGA+GGG | - | Chr2:56049858-56049877 | None:intergenic | 50.0% | |
| TTGGAGGGATAACCCATAGG+TGG | - | Chr2:56049843-56049862 | None:intergenic | 50.0% | |
| TTGTTGGGGATCATCCTTGG+AGG | - | Chr2:56049859-56049878 | None:intergenic | 50.0% | |
| ! | ACCTATGGGTTATCCCTCCA+AGG | + | Chr2:56049842-56049861 | MsG0280009673.01.T01:CDS | 50.0% |
| ! | AGGGGTGATGGTTTCTGGAA+AGG | + | Chr2:56049903-56049922 | MsG0280009673.01.T01:CDS | 50.0% |
| AGCTGTTCCACCACCACCTA+TGG | + | Chr2:56049827-56049846 | MsG0280009673.01.T01:CDS | 55.0% | |
| GAGGGATAACCCATAGGTGG+TGG | - | Chr2:56049840-56049859 | None:intergenic | 55.0% | |
| GCTGTTCCACCACCACCTAT+GGG | + | Chr2:56049828-56049847 | MsG0280009673.01.T01:CDS | 55.0% | |
| GGGTTGTTCTGACTCACTGC+TGG | - | Chr2:56049804-56049823 | None:intergenic | 55.0% | |
| ! | CTAGCAGGGGTGATGGTTTC+TGG | + | Chr2:56049898-56049917 | MsG0280009673.01.T01:CDS | 55.0% |
| ! | GGATAACCCATAGGTGGTGG+TGG | - | Chr2:56049837-56049856 | None:intergenic | 55.0% |
| !! | GTGGTGGTGGAACAGCTTGA+GGG | - | Chr2:56049824-56049843 | None:intergenic | 55.0% |
| !! | GGTGGTGGTGGAACAGCTTG+AGG | - | Chr2:56049825-56049844 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 56049416 | 56050614 | 56049416 | ID=MsG0280009673.01;Name=MsG0280009673.01 |
| Chr2 | mRNA | 56049416 | 56050614 | 56049416 | ID=MsG0280009673.01.T01;Parent=MsG0280009673.01;Name=MsG0280009673.01.T01;_AED=0.29;_eAED=0.29;_QI=139|1|1|1|0|0|3|210|72 |
| Chr2 | exon | 56050359 | 56050614 | 56050359 | ID=MsG0280009673.01.T01:exon:37876;Parent=MsG0280009673.01.T01 |
| Chr2 | exon | 56049790 | 56049928 | 56049790 | ID=MsG0280009673.01.T01:exon:37875;Parent=MsG0280009673.01.T01 |
| Chr2 | exon | 56049416 | 56049588 | 56049416 | ID=MsG0280009673.01.T01:exon:37874;Parent=MsG0280009673.01.T01 |
| Chr2 | five_prime_UTR | 56049416 | 56049554 | 56049416 | ID=MsG0280009673.01.T01:five_prime_utr;Parent=MsG0280009673.01.T01 |
| Chr2 | CDS | 56049555 | 56049588 | 56049555 | ID=MsG0280009673.01.T01:cds;Parent=MsG0280009673.01.T01 |
| Chr2 | CDS | 56049790 | 56049928 | 56049790 | ID=MsG0280009673.01.T01:cds;Parent=MsG0280009673.01.T01 |
| Chr2 | CDS | 56050359 | 56050404 | 56050359 | ID=MsG0280009673.01.T01:cds;Parent=MsG0280009673.01.T01 |
| Chr2 | three_prime_UTR | 56050405 | 56050614 | 56050405 | ID=MsG0280009673.01.T01:three_prime_utr;Parent=MsG0280009673.01.T01 |
| Gene Sequence |
| Protein sequence |
