Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012173.01.T01 | AES68854.1 | 98.611 | 72 | 1 | 0 | 1 | 72 | 1 | 72 | 5.35E-39 | 134 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012173.01.T01 | G7IYT5 | 98.611 | 72 | 1 | 0 | 1 | 72 | 1 | 72 | 2.56e-39 | 134 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000417.01 | MsG0380012173.01 | 0.810223 | 1.282488e-50 | 7.686602e-48 |
| MsG0180001925.01 | MsG0380012173.01 | 0.814993 | 1.160517e-51 | 7.904799e-49 |
| MsG0180002099.01 | MsG0380012173.01 | 0.806855 | 6.717560e-50 | 3.685391e-47 |
| MsG0180005811.01 | MsG0380012173.01 | 0.823154 | 1.613795e-53 | 1.378442e-50 |
| MsG0180006227.01 | MsG0380012173.01 | 0.805323 | 1.410980e-49 | 7.442755e-47 |
| MsG0380012173.01 | MsG0380013501.01 | 0.804648 | 1.953308e-49 | 1.012605e-46 |
| MsG0380012173.01 | MsG0380015974.01 | 0.800098 | 1.690333e-48 | 7.802902e-46 |
| MsG0380012173.01 | MsG0380017406.01 | 0.810928 | 9.032072e-51 | 5.516525e-48 |
| MsG0380012173.01 | MsG0480022591.01 | 0.818319 | 2.085482e-52 | 1.555935e-49 |
| MsG0380012173.01 | MsG0480022626.01 | 0.800494 | 1.403740e-48 | 6.545511e-46 |
| MsG0380012173.01 | MsG0580026362.01 | 0.810657 | 1.033836e-50 | 6.268344e-48 |
| MsG0380012173.01 | MsG0780039838.01 | 0.811565 | 6.569469e-51 | 4.080253e-48 |
| MsG0380012173.01 | MsG0880043069.01 | 0.804138 | 2.494472e-49 | 1.276281e-46 |
| MsG0380012173.01 | MsG0880046630.01 | 0.807122 | 5.896451e-50 | 3.257244e-47 |
| MsG0280010227.01 | MsG0380012173.01 | 0.816148 | 6.419264e-52 | 4.512792e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380012173.01.T01 | MTR_3g016200 | 98.611 | 72 | 1 | 0 | 1 | 72 | 1 | 72 | 6.49e-43 | 134 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCACGTCTTGGTGGTAAAAC+TGG | 0.233567 | 3:+12156184 | None:intergenic |
| GGCGGCGGCCTGCCCTTTCT+TGG | 0.267709 | 3:+12156220 | None:intergenic |
| CCCTTTCTTGGTGGTAAAAC+AGG | 0.309079 | 3:+12156232 | None:intergenic |
| ATTCTCATTCCTCAAGGGTT+TGG | 0.371404 | 3:-12156280 | MsG0380012173.01.T01:CDS |
| GGGGTAGGAGGGACCGGCGG+CGG | 0.404972 | 3:+12156205 | None:intergenic |
| GGCGGTGGCCTGCCACGTCT+TGG | 0.433805 | 3:+12156172 | None:intergenic |
| AAAACTGGGGTAGGAGGGAC+CGG | 0.461257 | 3:+12156199 | None:intergenic |
| CACGTCTTGGTGGTAAAACT+GGG | 0.469712 | 3:+12156185 | None:intergenic |
| GGCGGCCTGCCCTTTCTTGG+TGG | 0.492551 | 3:+12156223 | None:intergenic |
| GGTTTGTACACAGGTGGGAC+TGG | 0.503859 | 3:+12156151 | None:intergenic |
| ACTGGGGTAGGAGGGACCGG+CGG | 0.506401 | 3:+12156202 | None:intergenic |
| AAGAAAGGGCAGGCCGCCGC+CGG | 0.507549 | 3:-12156218 | MsG0380012173.01.T01:CDS |
| GGTGGTAAAACTGGGGTAGG+AGG | 0.520380 | 3:+12156193 | None:intergenic |
| TACACAGGTGGGACTGGCGG+TGG | 0.520676 | 3:+12156157 | None:intergenic |
| ATGAACTAAGGCTTATCAAT+TGG | 0.523294 | 3:+12156091 | None:intergenic |
| TCTCTAATTCTCATTCCTCA+AGG | 0.528197 | 3:-12156286 | MsG0380012173.01.T01:CDS |
| CTCTAATTCTCATTCCTCAA+GGG | 0.528769 | 3:-12156285 | MsG0380012173.01.T01:CDS |
| GGAACAACACTAGAAAGGAT+AGG | 0.537889 | 3:+12156314 | None:intergenic |
| TAAACAGTTGGTTTGTACAC+AGG | 0.551898 | 3:+12156142 | None:intergenic |
| GAACAACACTAGAAAGGATA+GGG | 0.553978 | 3:+12156315 | None:intergenic |
| TGCAAGGAACAACACTAGAA+AGG | 0.555666 | 3:+12156309 | None:intergenic |
| GTGGTAAAACTGGGGTAGGA+GGG | 0.564531 | 3:+12156194 | None:intergenic |
| GGTGGCCTGCCACGTCTTGG+TGG | 0.575431 | 3:+12156175 | None:intergenic |
| ACAGTTGGTTTGTACACAGG+TGG | 0.581693 | 3:+12156145 | None:intergenic |
| CTTGGTGGTAAAACTGGGGT+AGG | 0.595325 | 3:+12156190 | None:intergenic |
| GAATGAGAATTAGAGATGCA+AGG | 0.608069 | 3:+12156293 | None:intergenic |
| CAGTTGGTTTGTACACAGGT+GGG | 0.623331 | 3:+12156146 | None:intergenic |
| TTGTACACAGGTGGGACTGG+CGG | 0.640198 | 3:+12156154 | None:intergenic |
| TCATGAATACCAAACCCTTG+AGG | 0.659039 | 3:+12156271 | None:intergenic |
| ACGTCTTGGTGGTAAAACTG+GGG | 0.729690 | 3:+12156186 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTGGCTTATAAACAGT+TGG | + | Chr3:12156297-12156316 | None:intergenic | 25.0% |
| !!! | GACTTGTTCACTTGATTTTT+TGG | + | Chr3:12156312-12156331 | None:intergenic | 30.0% |
| CTCTAATTCTCATTCCTCAA+GGG | - | Chr3:12156139-12156158 | MsG0380012173.01.T01:CDS | 35.0% | |
| GAACAACACTAGAAAGGATA+GGG | + | Chr3:12156112-12156131 | None:intergenic | 35.0% | |
| GAATGAGAATTAGAGATGCA+AGG | + | Chr3:12156134-12156153 | None:intergenic | 35.0% | |
| TCTCTAATTCTCATTCCTCA+AGG | - | Chr3:12156138-12156157 | MsG0380012173.01.T01:CDS | 35.0% | |
| !! | TAAACAGTTGGTTTGTACAC+AGG | + | Chr3:12156285-12156304 | None:intergenic | 35.0% |
| ATTCTCATTCCTCAAGGGTT+TGG | - | Chr3:12156144-12156163 | MsG0380012173.01.T01:CDS | 40.0% | |
| GGAACAACACTAGAAAGGAT+AGG | + | Chr3:12156113-12156132 | None:intergenic | 40.0% | |
| TCATGAATACCAAACCCTTG+AGG | + | Chr3:12156156-12156175 | None:intergenic | 40.0% | |
| TGCAAGGAACAACACTAGAA+AGG | + | Chr3:12156118-12156137 | None:intergenic | 40.0% | |
| ! | CCTGTTTTACCACCAAGAAA+GGG | - | Chr3:12156192-12156211 | MsG0380012173.01.T01:CDS | 40.0% |
| ! | TCCTGTTTTACCACCAAGAA+AGG | - | Chr3:12156191-12156210 | MsG0380012173.01.T01:CDS | 40.0% |
| ! | ACGTCTTGGTGGTAAAACTG+GGG | + | Chr3:12156241-12156260 | None:intergenic | 45.0% |
| ! | CACGTCTTGGTGGTAAAACT+GGG | + | Chr3:12156242-12156261 | None:intergenic | 45.0% |
| ! | CCCTTTCTTGGTGGTAAAAC+AGG | + | Chr3:12156195-12156214 | None:intergenic | 45.0% |
| ! | TTTTACCACCAAGAAAGGGC+AGG | - | Chr3:12156196-12156215 | MsG0380012173.01.T01:CDS | 45.0% |
| !! | ACAGTTGGTTTGTACACAGG+TGG | + | Chr3:12156282-12156301 | None:intergenic | 45.0% |
| !! | CAGTTGGTTTGTACACAGGT+GGG | + | Chr3:12156281-12156300 | None:intergenic | 45.0% |
| GTGGTAAAACTGGGGTAGGA+GGG | + | Chr3:12156233-12156252 | None:intergenic | 50.0% | |
| ! | CCACGTCTTGGTGGTAAAAC+TGG | + | Chr3:12156243-12156262 | None:intergenic | 50.0% |
| ! | CCAGTTTTACCACCAAGACG+TGG | - | Chr3:12156240-12156259 | MsG0380012173.01.T01:CDS | 50.0% |
| ! | CTTGGTGGTAAAACTGGGGT+AGG | + | Chr3:12156237-12156256 | None:intergenic | 50.0% |
| ! | TTTTACCACCAAGACGTGGC+AGG | - | Chr3:12156244-12156263 | MsG0380012173.01.T01:CDS | 50.0% |
| AAAACTGGGGTAGGAGGGAC+CGG | + | Chr3:12156228-12156247 | None:intergenic | 55.0% | |
| GGTGGTAAAACTGGGGTAGG+AGG | + | Chr3:12156234-12156253 | None:intergenic | 55.0% | |
| GGTTTGTACACAGGTGGGAC+TGG | + | Chr3:12156276-12156295 | None:intergenic | 55.0% | |
| TTGTACACAGGTGGGACTGG+CGG | + | Chr3:12156273-12156292 | None:intergenic | 55.0% | |
| ! | TACACAGGTGGGACTGGCGG+TGG | + | Chr3:12156270-12156289 | None:intergenic | 65.0% |
| AAGAAAGGGCAGGCCGCCGC+CGG | - | Chr3:12156206-12156225 | MsG0380012173.01.T01:CDS | 70.0% | |
| ACTGGGGTAGGAGGGACCGG+CGG | + | Chr3:12156225-12156244 | None:intergenic | 70.0% | |
| GGCGGCCTGCCCTTTCTTGG+TGG | + | Chr3:12156204-12156223 | None:intergenic | 70.0% | |
| GGTGGCCTGCCACGTCTTGG+TGG | + | Chr3:12156252-12156271 | None:intergenic | 70.0% | |
| GGCGGCGGCCTGCCCTTTCT+TGG | + | Chr3:12156207-12156226 | None:intergenic | 75.0% | |
| GGCGGTGGCCTGCCACGTCT+TGG | + | Chr3:12156255-12156274 | None:intergenic | 75.0% | |
| ! | GGGGTAGGAGGGACCGGCGG+CGG | + | Chr3:12156222-12156241 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 12156096 | 12156350 | 12156096 | ID=MsG0380012173.01;Name=MsG0380012173.01 |
| Chr3 | mRNA | 12156096 | 12156350 | 12156096 | ID=MsG0380012173.01.T01;Parent=MsG0380012173.01;Name=MsG0380012173.01.T01;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|84 |
| Chr3 | exon | 12156096 | 12156350 | 12156096 | ID=MsG0380012173.01.T01:exon:728;Parent=MsG0380012173.01.T01 |
| Chr3 | CDS | 12156096 | 12156350 | 12156096 | ID=MsG0380012173.01.T01:cds;Parent=MsG0380012173.01.T01 |
| Gene Sequence |
| Protein sequence |
