AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017406.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 49 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTTGGAGGTTGGGGAGTT+TGG 0.094673 3:+95898640 MsG0380017406.01.T01:CDS
ATTTGGAGGTTGGGGAGTTT+GGG 0.158090 3:+95898641 MsG0380017406.01.T01:CDS
ATTTGTGGGGACTCGGATTA+TGG 0.233073 3:+95898682 MsG0380017406.01.T01:CDS
TGATGGTTGGGAGGGAGATT+TGG 0.248134 3:+95898624 MsG0380017406.01.T01:CDS
GGGAGGGAGATTTGGAGGTT+GGG 0.289496 3:+95898632 MsG0380017406.01.T01:CDS
GAGTTTGAGGTTTGGGGTGA+TGG 0.299689 3:+95898607 MsG0380017406.01.T01:CDS
GGAGGAGGGTAGGGAATTTG+TGG 0.302413 3:+95898667 MsG0380017406.01.T01:CDS
AGAGTTGGAGTTTGAGGTTT+GGG 0.305326 3:+95898600 MsG0380017406.01.T01:CDS
GAGTAGACGTAAAGAGGATT+TGG 0.310651 3:+95898822 MsG0380017406.01.T01:CDS
TGAGGTTTGGGGTGATGGTT+GGG 0.313696 3:+95898612 MsG0380017406.01.T01:CDS
TTTGGAGGTTGGGGAGTTTG+GGG 0.315155 3:+95898642 MsG0380017406.01.T01:CDS
GGAGGGAGATTTGGAGGTTG+GGG 0.331742 3:+95898633 MsG0380017406.01.T01:CDS
TTGAGGTTTGGGGTGATGGT+TGG 0.333131 3:+95898611 MsG0380017406.01.T01:CDS
TGGGAGGGAGATTTGGAGGT+TGG 0.335662 3:+95898631 MsG0380017406.01.T01:CDS
CGTAAAGAGGATTTGGGAAA+TGG 0.368659 3:+95898829 MsG0380017406.01.T01:CDS
AGAAATTCAACGACTATGAT+TGG 0.371223 3:+95898470 MsG0380017406.01.T01:CDS
TGGTTGGGAGGGAGATTTGG+AGG 0.401249 3:+95898627 MsG0380017406.01.T01:CDS
GAGAGTTGGAGTTTGAGGTT+TGG 0.402330 3:+95898599 MsG0380017406.01.T01:CDS
TTTGTGGGGACTCGGATTAT+GGG 0.403270 3:+95898683 MsG0380017406.01.T01:CDS
AGTAGACGTAAAGAGGATTT+GGG 0.403814 3:+95898823 MsG0380017406.01.T01:CDS
AAACACACACACGCAGATAG+AGG 0.404330 3:-95898764 None:intergenic
TGTGTGTGTTTGTGTTGTTT+TGG 0.421951 3:+95898776 MsG0380017406.01.T01:CDS
GGTTTGGGGTGATGGTTGGG+AGG 0.428537 3:+95898615 MsG0380017406.01.T01:CDS
GTAGGGAATTTGTGGGGACT+CGG 0.454663 3:+95898675 MsG0380017406.01.T01:CDS
GCCACACGATTTCATGAGGT+TGG 0.478501 3:+95898871 MsG0380017406.01.T01:CDS
GAAATTCAACGACTATGATT+GGG 0.478683 3:+95898471 MsG0380017406.01.T01:CDS
TTAGTGGTGAGGGGAGTGAT+TGG 0.480574 3:+95898800 MsG0380017406.01.T01:CDS
TGATTGGAGTAGACGTAAAG+AGG 0.480713 3:+95898816 MsG0380017406.01.T01:CDS
TTGGAGGTTGGGGAGTTTGG+GGG 0.481174 3:+95898643 MsG0380017406.01.T01:CDS
GTTTGGGGTGATGGTTGGGA+GGG 0.488598 3:+95898616 MsG0380017406.01.T01:CDS
TGCAATGGCCTCAATGATCA+TGG 0.509890 3:-95898717 None:intergenic
GGAAGTTGCCGCTAGGGTCA+TGG 0.514941 3:+95898565 MsG0380017406.01.T01:CDS
AGAGAGGGAAGTTGCCGCTA+GGG 0.535819 3:+95898559 MsG0380017406.01.T01:CDS
GAGGAGGGTAGGGAATTTGT+GGG 0.539659 3:+95898668 MsG0380017406.01.T01:CDS
AACACACACACGCAGATAGA+GGG 0.552472 3:-95898763 None:intergenic
AAGAGAGGGAAGTTGCCGCT+AGG 0.555867 3:+95898558 MsG0380017406.01.T01:CDS
GAGTTGGAGTTTGAGGTTTG+GGG 0.565218 3:+95898601 MsG0380017406.01.T01:CDS
AGCGAGGGAAGGTGGTGCGA+AGG 0.566516 3:+95898532 MsG0380017406.01.T01:CDS
TGAAGAAGCCATGATCATTG+AGG 0.572864 3:+95898709 MsG0380017406.01.T01:CDS
CTTTGCCACACGATTTCATG+AGG 0.574481 3:+95898867 MsG0380017406.01.T01:CDS
TGGTGCGAAGGCGAAAGAGA+GGG 0.595311 3:+95898544 MsG0380017406.01.T01:CDS
CGACTATGATTGGGAGAGTG+AGG 0.595912 3:+95898480 MsG0380017406.01.T01:CDS
GTGGTGCGAAGGCGAAAGAG+AGG 0.606516 3:+95898543 MsG0380017406.01.T01:CDS
AGGAGGGTAGGGAATTTGTG+GGG 0.641672 3:+95898669 MsG0380017406.01.T01:CDS
TCCAACCTCATGAAATCGTG+TGG 0.643684 3:-95898872 None:intergenic
AAAGAGGATTTGGGAAATGG+AGG 0.643919 3:+95898832 MsG0380017406.01.T01:CDS
GATCATTGAGGCCATTGCAA+TGG 0.651912 3:+95898721 MsG0380017406.01.T01:CDS
GAGGTTGAAATCGAAGAGTG+CGG 0.686862 3:+95898499 MsG0380017406.01.T01:CDS
ACTCAAATCCATGACCCTAG+CGG 0.699556 3:-95898573 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGAAATTCAACGACTATGAT+TGG + Chr3:95898470-95898489 MsG0380017406.01.T01:CDS 30.0%
GAAATTCAACGACTATGATT+GGG + Chr3:95898471-95898490 MsG0380017406.01.T01:CDS 30.0%
AGTAGACGTAAAGAGGATTT+GGG + Chr3:95898823-95898842 MsG0380017406.01.T01:CDS 35.0%
TCCAAAAATCACCATTGCAA+TGG - Chr3:95898735-95898754 None:intergenic 35.0%
!! ATTGCAATGGTGATTTTTGG+AGG + Chr3:95898734-95898753 MsG0380017406.01.T01:CDS 35.0%
!!! TGGATTTGAGTTTTGAGAGT+TGG + Chr3:95898585-95898604 MsG0380017406.01.T01:CDS 35.0%
!!! TGTGTGTGTTTGTGTTGTTT+TGG + Chr3:95898776-95898795 MsG0380017406.01.T01:CDS 35.0%
!!! TTTGTGTTGTTTTGGCTTAG+TGG + Chr3:95898784-95898803 MsG0380017406.01.T01:CDS 35.0%
AAAGAGGATTTGGGAAATGG+AGG + Chr3:95898832-95898851 MsG0380017406.01.T01:CDS 40.0%
CGTAAAGAGGATTTGGGAAA+TGG + Chr3:95898829-95898848 MsG0380017406.01.T01:CDS 40.0%
GAGTAGACGTAAAGAGGATT+TGG + Chr3:95898822-95898841 MsG0380017406.01.T01:CDS 40.0%
TGAAGAAGCCATGATCATTG+AGG + Chr3:95898709-95898728 MsG0380017406.01.T01:CDS 40.0%
TGATTGGAGTAGACGTAAAG+AGG + Chr3:95898816-95898835 MsG0380017406.01.T01:CDS 40.0%
! AGAGTTGGAGTTTGAGGTTT+GGG + Chr3:95898600-95898619 MsG0380017406.01.T01:CDS 40.0%
! GCCATTGCAATGGTGATTTT+TGG + Chr3:95898731-95898750 MsG0380017406.01.T01:CDS 40.0%
!!! GTTTTGAGAGTTGGAGTTTG+AGG + Chr3:95898594-95898613 MsG0380017406.01.T01:CDS 40.0%
!!! TTGTTTTGGCTTAGTGGTGA+GGG + Chr3:95898790-95898809 MsG0380017406.01.T01:CDS 40.0%
AAACACACACACGCAGATAG+AGG - Chr3:95898767-95898786 None:intergenic 45.0%
AACACACACACGCAGATAGA+GGG - Chr3:95898766-95898785 None:intergenic 45.0%
ACTCAAATCCATGACCCTAG+CGG - Chr3:95898576-95898595 None:intergenic 45.0%
ATTTGGAGGTTGGGGAGTTT+GGG + Chr3:95898641-95898660 MsG0380017406.01.T01:CDS 45.0%
ATTTGTGGGGACTCGGATTA+TGG + Chr3:95898682-95898701 MsG0380017406.01.T01:CDS 45.0%
CTTTGCCACACGATTTCATG+AGG + Chr3:95898867-95898886 MsG0380017406.01.T01:CDS 45.0%
GAGGTTGAAATCGAAGAGTG+CGG + Chr3:95898499-95898518 MsG0380017406.01.T01:CDS 45.0%
GATCATTGAGGCCATTGCAA+TGG + Chr3:95898721-95898740 MsG0380017406.01.T01:CDS 45.0%
TCCAACCTCATGAAATCGTG+TGG - Chr3:95898875-95898894 None:intergenic 45.0%
TGCAATGGCCTCAATGATCA+TGG - Chr3:95898720-95898739 None:intergenic 45.0%
TTTGTGGGGACTCGGATTAT+GGG + Chr3:95898683-95898702 MsG0380017406.01.T01:CDS 45.0%
! GAGAGTTGGAGTTTGAGGTT+TGG + Chr3:95898599-95898618 MsG0380017406.01.T01:CDS 45.0%
! GAGTTGGAGTTTGAGGTTTG+GGG + Chr3:95898601-95898620 MsG0380017406.01.T01:CDS 45.0%
!!! GTTGTTTTGGCTTAGTGGTG+AGG + Chr3:95898789-95898808 MsG0380017406.01.T01:CDS 45.0%
!!! TGTTTTGGCTTAGTGGTGAG+GGG + Chr3:95898791-95898810 MsG0380017406.01.T01:CDS 45.0%
AGGAGGGTAGGGAATTTGTG+GGG + Chr3:95898669-95898688 MsG0380017406.01.T01:CDS 50.0%
CGACTATGATTGGGAGAGTG+AGG + Chr3:95898480-95898499 MsG0380017406.01.T01:CDS 50.0%
GAGGAGGGTAGGGAATTTGT+GGG + Chr3:95898668-95898687 MsG0380017406.01.T01:CDS 50.0%
GATTTGGAGGTTGGGGAGTT+TGG + Chr3:95898640-95898659 MsG0380017406.01.T01:CDS 50.0%
GCCACACGATTTCATGAGGT+TGG + Chr3:95898871-95898890 MsG0380017406.01.T01:CDS 50.0%
GTAGGGAATTTGTGGGGACT+CGG + Chr3:95898675-95898694 MsG0380017406.01.T01:CDS 50.0%
TGATGGTTGGGAGGGAGATT+TGG + Chr3:95898624-95898643 MsG0380017406.01.T01:CDS 50.0%
TTAGTGGTGAGGGGAGTGAT+TGG + Chr3:95898800-95898819 MsG0380017406.01.T01:CDS 50.0%
TTTGGAGGTTGGGGAGTTTG+GGG + Chr3:95898642-95898661 MsG0380017406.01.T01:CDS 50.0%
! GAGTTTGAGGTTTGGGGTGA+TGG + Chr3:95898607-95898626 MsG0380017406.01.T01:CDS 50.0%
! TGAGGTTTGGGGTGATGGTT+GGG + Chr3:95898612-95898631 MsG0380017406.01.T01:CDS 50.0%
! TTGAGGTTTGGGGTGATGGT+TGG + Chr3:95898611-95898630 MsG0380017406.01.T01:CDS 50.0%
! TTTTCGAAAGCGAGGGAAGG+TGG + Chr3:95898524-95898543 MsG0380017406.01.T01:CDS 50.0%
!!! GCTTTTTCGAAAGCGAGGGA+AGG + Chr3:95898521-95898540 MsG0380017406.01.T01:CDS 50.0%
!!! TGCGGCTTTTTCGAAAGCGA+GGG + Chr3:95898517-95898536 MsG0380017406.01.T01:CDS 50.0%
AAGAGAGGGAAGTTGCCGCT+AGG + Chr3:95898558-95898577 MsG0380017406.01.T01:CDS 55.0%
AGAGAGGGAAGTTGCCGCTA+GGG + Chr3:95898559-95898578 MsG0380017406.01.T01:CDS 55.0%
GGAGGAGGGTAGGGAATTTG+TGG + Chr3:95898667-95898686 MsG0380017406.01.T01:CDS 55.0%
GGAGGGAGATTTGGAGGTTG+GGG + Chr3:95898633-95898652 MsG0380017406.01.T01:CDS 55.0%
GGGAGGGAGATTTGGAGGTT+GGG + Chr3:95898632-95898651 MsG0380017406.01.T01:CDS 55.0%
TGGGAGGGAGATTTGGAGGT+TGG + Chr3:95898631-95898650 MsG0380017406.01.T01:CDS 55.0%
TGGTGCGAAGGCGAAAGAGA+GGG + Chr3:95898544-95898563 MsG0380017406.01.T01:CDS 55.0%
TGGTTGGGAGGGAGATTTGG+AGG + Chr3:95898627-95898646 MsG0380017406.01.T01:CDS 55.0%
TTGGAGGTTGGGGAGTTTGG+GGG + Chr3:95898643-95898662 MsG0380017406.01.T01:CDS 55.0%
! GTTTGGGGTGATGGTTGGGA+GGG + Chr3:95898616-95898635 MsG0380017406.01.T01:CDS 55.0%
!!! GTGCGGCTTTTTCGAAAGCG+AGG + Chr3:95898516-95898535 MsG0380017406.01.T01:CDS 55.0%
GGAAGTTGCCGCTAGGGTCA+TGG + Chr3:95898565-95898584 MsG0380017406.01.T01:CDS 60.0%
GTGGTGCGAAGGCGAAAGAG+AGG + Chr3:95898543-95898562 MsG0380017406.01.T01:CDS 60.0%
TTTGGGGGTGGAGGAGGGTA+GGG + Chr3:95898658-95898677 MsG0380017406.01.T01:CDS 60.0%
! GGTTTGGGGTGATGGTTGGG+AGG + Chr3:95898615-95898634 MsG0380017406.01.T01:CDS 60.0%
AGCGAGGGAAGGTGGTGCGA+AGG + Chr3:95898532-95898551 MsG0380017406.01.T01:CDS 65.0%
GAGGTTGGGGAGTTTGGGGG+TGG + Chr3:95898646-95898665 MsG0380017406.01.T01:CDS 65.0%
GGGAGTTTGGGGGTGGAGGA+GGG + Chr3:95898653-95898672 MsG0380017406.01.T01:CDS 65.0%
GTTGGGGAGTTTGGGGGTGG+AGG + Chr3:95898649-95898668 MsG0380017406.01.T01:CDS 65.0%
GTTTGGGGGTGGAGGAGGGT+AGG + Chr3:95898657-95898676 MsG0380017406.01.T01:CDS 65.0%
GGGGAGTTTGGGGGTGGAGG+AGG + Chr3:95898652-95898671 MsG0380017406.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr3 gene 95898461 95898898 95898461 ID=MsG0380017406.01;Name=MsG0380017406.01
Chr3 mRNA 95898461 95898898 95898461 ID=MsG0380017406.01.T01;Parent=MsG0380017406.01;Name=MsG0380017406.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|145
Chr3 exon 95898461 95898898 95898461 ID=MsG0380017406.01.T01:exon:1760;Parent=MsG0380017406.01.T01
Chr3 CDS 95898461 95898898 95898461 ID=MsG0380017406.01.T01:cds;Parent=MsG0380017406.01.T01
Gene Sequence

>MsG0380017406.01.T01

ATGAGGAAGAGAAATTCAACGACTATGATTGGGAGAGTGAGGTTGAAATCGAAGAGTGCGGCTTTTTCGAAAGCGAGGGAAGGTGGTGCGAAGGCGAAAGAGAGGGAAGTTGCCGCTAGGGTCATGGATTTGAGTTTTGAGAGTTGGAGTTTGAGGTTTGGGGTGATGGTTGGGAGGGAGATTTGGAGGTTGGGGAGTTTGGGGGTGGAGGAGGGTAGGGAATTTGTGGGGACTCGGATTATGGGTTTTGAAGAAGCCATGATCATTGAGGCCATTGCAATGGTGATTTTTGGAGGATCTCTCCCTCTATCTGCGTGTGTGTGTTTGTGTTGTTTTGGCTTAGTGGTGAGGGGAGTGATTGGAGTAGACGTAAAGAGGATTTGGGAAATGGAGGTCTATCTGAATTCTTTGCCACACGATTTCATGAGGTTGGAGTGA

Protein sequence

>MsG0380017406.01.T01

MRKRNSTTMIGRVRLKSKSAAFSKAREGGAKAKEREVAARVMDLSFESWSLRFGVMVGREIWRLGSLGVEEGREFVGTRIMGFEEAMIIEAIAMVIFGGSLPLSACVCLCCFGLVVRGVIGVDVKRIWEMEVYLNSLPHDFMRLE*