Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014151.01.T01 | XP_003600087.1 | 95 | 80 | 4 | 0 | 2 | 81 | 18 | 97 | 9.64E-48 | 156 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014151.01.T01 | G7IXN7 | 95.000 | 80 | 4 | 0 | 2 | 81 | 18 | 97 | 4.60e-48 | 156 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048410.01 | MsG0380014151.01 | 0.802251 | 6.128863e-49 | 2.988048e-46 |
| MsG0180000293.01 | MsG0380014151.01 | 0.802885 | 4.536719e-49 | 2.247785e-46 |
| MsG0180004005.01 | MsG0380014151.01 | 0.807453 | 5.017106e-50 | 2.795648e-47 |
| MsG0180004651.01 | MsG0380014151.01 | 0.801762 | 7.728325e-49 | 3.720965e-46 |
| MsG0180004762.01 | MsG0380014151.01 | 0.812485 | 4.140501e-51 | 2.635981e-48 |
| MsG0180005407.01 | MsG0380014151.01 | 0.800724 | 1.260306e-48 | 5.910923e-46 |
| MsG0180005813.01 | MsG0380014151.01 | 0.812038 | 5.183731e-51 | 3.260211e-48 |
| MsG0180006047.01 | MsG0380014151.01 | 0.814233 | 1.709880e-51 | 1.140973e-48 |
| MsG0380014151.01 | MsG0380015181.01 | 0.808127 | 3.607688e-50 | 2.046002e-47 |
| MsG0380014151.01 | MsG0480018126.01 | 0.810242 | 1.270679e-50 | 7.619956e-48 |
| MsG0380014151.01 | MsG0480021298.01 | 0.813515 | 2.461418e-51 | 1.610807e-48 |
| MsG0380014151.01 | MsG0480023224.01 | 0.840892 | 6.613136e-58 | 9.553948e-55 |
| MsG0380014151.01 | MsG0480023517.01 | 0.804887 | 1.740581e-49 | 9.080134e-47 |
| MsG0380014151.01 | MsG0480023733.01 | 0.800163 | 1.639291e-48 | 7.579585e-46 |
| MsG0380014151.01 | MsG0580024205.01 | 0.801243 | 9.872744e-49 | 4.691851e-46 |
| MsG0380014151.01 | MsG0580025989.01 | 0.822239 | 2.635304e-53 | 2.193213e-50 |
| MsG0380014151.01 | MsG0580026225.01 | 0.801946 | 7.083855e-49 | 3.426672e-46 |
| MsG0380014151.01 | MsG0580027345.01 | 0.809487 | 1.846576e-50 | 1.085580e-47 |
| MsG0380014151.01 | MsG0580029658.01 | 0.824161 | 9.379262e-54 | 8.242508e-51 |
| MsG0380014151.01 | MsG0680033971.01 | 0.822705 | 2.053737e-53 | 1.732093e-50 |
| MsG0380014151.01 | MsG0780040691.01 | 0.810189 | 1.304493e-50 | 7.811580e-48 |
| MsG0380014151.01 | MsG0880042052.01 | 0.806117 | 9.612278e-50 | 5.174812e-47 |
| MsG0380014151.01 | MsG0880042967.01 | 0.818572 | 1.827573e-52 | 1.373036e-49 |
| MsG0380014151.01 | MsG0880043001.01 | 0.842187 | 3.014436e-58 | 4.534203e-55 |
| MsG0380014151.01 | MsG0880045106.01 | 0.840606 | 7.859438e-58 | 1.125298e-54 |
| MsG0380014151.01 | MsG0880046214.01 | 0.844497 | 7.289632e-59 | 1.179065e-55 |
| MsG0380014151.01 | MsG0880046317.01 | 0.811778 | 5.904081e-51 | 3.687647e-48 |
| MsG0380014151.01 | MsG0880046329.01 | 0.831433 | 1.674038e-55 | 1.816273e-52 |
| MsG0380014151.01 | MsG0880046642.01 | 0.805967 | 1.033617e-49 | 5.543364e-47 |
| MsG0380014151.01 | MsG0880047590.01 | 0.815315 | 9.845208e-52 | 6.764900e-49 |
| MsG0280007585.01 | MsG0380014151.01 | 0.821116 | 4.789611e-53 | 3.862036e-50 |
| MsG0280007648.01 | MsG0380014151.01 | 0.813588 | 2.372013e-51 | 1.555384e-48 |
| MsG0280008039.01 | MsG0380014151.01 | 0.811623 | 6.380459e-51 | 3.968766e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014151.01.T01 | MTR_3g051610 | 95.000 | 80 | 4 | 0 | 2 | 81 | 18 | 97 | 1.17e-51 | 156 |
| MsG0380014151.01.T01 | MTR_3g051530 | 86.250 | 80 | 11 | 0 | 2 | 81 | 20 | 99 | 7.83e-47 | 144 |
| MsG0380014151.01.T01 | MTR_3g051570 | 85.000 | 80 | 12 | 0 | 2 | 81 | 22 | 101 | 5.68e-45 | 140 |
| MsG0380014151.01.T01 | MTR_3g051510 | 82.500 | 80 | 14 | 0 | 2 | 81 | 20 | 99 | 9.69e-44 | 137 |
| MsG0380014151.01.T01 | MTR_5g092710 | 55.072 | 69 | 30 | 1 | 9 | 77 | 15 | 82 | 9.48e-21 | 78.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014151.01.T01 | AT3G20340 | 46.667 | 60 | 29 | 2 | 25 | 81 | 56 | 115 | 8.56e-11 | 53.9 |
Find 17 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTCAAGCTCAATCAGCTTC+AGG | 0.287651 | 3:-50479122 | None:intergenic |
| CATTACTATGGTTCATCAAT+TGG | 0.304856 | 3:-50479052 | None:intergenic |
| GGCACCGGCACAGTTGCTTT+TGG | 0.315364 | 3:-50478928 | None:intergenic |
| TCGAATCACTTCAAAGATCT+TGG | 0.334518 | 3:+50479078 | MsG0380014151.01.T01:CDS |
| CTTCACCTCAGTCGTTGCTT+TGG | 0.359740 | 3:-50478949 | None:intergenic |
| GTGATTCGAAGCCATTACTA+TGG | 0.408236 | 3:-50479064 | None:intergenic |
| GTTAAGCAAAGTGGACATAA+AGG | 0.411060 | 3:+50479004 | MsG0380014151.01.T01:CDS |
| ATTACTATGGTTCATCAATT+GGG | 0.424384 | 3:-50479051 | None:intergenic |
| CTCAGTCGTTGCTTTGGCAC+CGG | 0.469921 | 3:-50478943 | None:intergenic |
| TCAGGGATGCTCTGAAGTGC+AGG | 0.475944 | 3:-50479104 | None:intergenic |
| TTCAAGCTCAATCAGCTTCA+GGG | 0.480069 | 3:-50479121 | None:intergenic |
| TGCATCAGAAGGTGACTCTC+CGG | 0.510614 | 3:+50478905 | MsG0380014151.01.T01:CDS |
| AGAAGAGTTGTTAAGCAAAG+TGG | 0.524820 | 3:+50478995 | MsG0380014151.01.T01:CDS |
| CCGGCCAAAAGCAACTGTGC+CGG | 0.538980 | 3:+50478924 | MsG0380014151.01.T01:CDS |
| CAATTGATGAACCATAGTAA+TGG | 0.602665 | 3:+50479053 | MsG0380014151.01.T01:CDS |
| GGACATAAAGGAGTTGAGAG+TGG | 0.618785 | 3:+50479016 | MsG0380014151.01.T01:CDS |
| CGGTGCCAAAGCAACGACTG+AGG | 0.681594 | 3:+50478944 | MsG0380014151.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTACTATGGTTCATCAATT+GGG | - | Chr3:50479054-50479073 | None:intergenic | 25.0% |
| CAATTGATGAACCATAGTAA+TGG | + | Chr3:50479053-50479072 | MsG0380014151.01.T01:CDS | 30.0% | |
| CATTACTATGGTTCATCAAT+TGG | - | Chr3:50479055-50479074 | None:intergenic | 30.0% | |
| AGAAGAGTTGTTAAGCAAAG+TGG | + | Chr3:50478995-50479014 | MsG0380014151.01.T01:CDS | 35.0% | |
| GTTAAGCAAAGTGGACATAA+AGG | + | Chr3:50479004-50479023 | MsG0380014151.01.T01:CDS | 35.0% | |
| TCGAATCACTTCAAAGATCT+TGG | + | Chr3:50479078-50479097 | MsG0380014151.01.T01:CDS | 35.0% | |
| GTGATTCGAAGCCATTACTA+TGG | - | Chr3:50479067-50479086 | None:intergenic | 40.0% | |
| ! | TGGGATTTTCATGCATCAGA+AGG | + | Chr3:50478894-50478913 | MsG0380014151.01.T01:CDS | 40.0% |
| GGACATAAAGGAGTTGAGAG+TGG | + | Chr3:50479016-50479035 | MsG0380014151.01.T01:CDS | 45.0% | |
| CTTCACCTCAGTCGTTGCTT+TGG | - | Chr3:50478952-50478971 | None:intergenic | 50.0% | |
| TGCATCAGAAGGTGACTCTC+CGG | + | Chr3:50478905-50478924 | MsG0380014151.01.T01:CDS | 50.0% | |
| ! | CTCAGTCGTTGCTTTGGCAC+CGG | - | Chr3:50478946-50478965 | None:intergenic | 55.0% |
| !! | TCAGGGATGCTCTGAAGTGC+AGG | - | Chr3:50479107-50479126 | None:intergenic | 55.0% |
| CCGGCCAAAAGCAACTGTGC+CGG | + | Chr3:50478924-50478943 | MsG0380014151.01.T01:CDS | 60.0% | |
| ! | CGGTGCCAAAGCAACGACTG+AGG | + | Chr3:50478944-50478963 | MsG0380014151.01.T01:CDS | 60.0% |
| !!! | CCGGCACAGTTGCTTTTGGC+CGG | - | Chr3:50478927-50478946 | None:intergenic | 60.0% |
| !!! | GGCACCGGCACAGTTGCTTT+TGG | - | Chr3:50478931-50478950 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 50478891 | 50479136 | 50478891 | ID=MsG0380014151.01;Name=MsG0380014151.01 |
| Chr3 | mRNA | 50478891 | 50479136 | 50478891 | ID=MsG0380014151.01.T01;Parent=MsG0380014151.01;Name=MsG0380014151.01.T01;_AED=0.32;_eAED=0.32;_QI=0|-1|0|1|-1|1|1|0|81 |
| Chr3 | exon | 50478891 | 50479136 | 50478891 | ID=MsG0380014151.01.T01:exon:9701;Parent=MsG0380014151.01.T01 |
| Chr3 | CDS | 50478891 | 50479136 | 50478891 | ID=MsG0380014151.01.T01:cds;Parent=MsG0380014151.01.T01 |
| Gene Sequence |
| Protein sequence |
