AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000293.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000293.01.T01 MTR_1g018790 97.351 151 4 0 1 151 379 529 2.83e-99 296
MsG0180000293.01.T01 MTR_4g094932 47.297 148 77 1 2 148 365 512 4.17e-36 130
MsG0180000293.01.T02 MTR_1g018790 96.078 102 4 0 1 102 428 529 5.34e-53 174
MsG0180000293.01.T02 MTR_4g094932 40.000 100 59 1 1 99 413 512 2.81e-15 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000293.01.T01 AT4G07400 61.644 146 54 1 2 147 390 533 1.42e-49 167
MsG0180000293.01.T01 AT1G47056 58.170 153 60 2 1 151 356 506 1.10e-47 161
MsG0180000293.01.T01 AT5G67250 50.658 152 70 2 2 151 361 509 1.89e-30 115
MsG0180000293.01.T01 AT3G50080 46.405 153 77 2 2 151 355 505 3.48e-28 108
MsG0180000293.01.T02 AT4G07400 56.122 98 41 1 1 98 438 533 4.61e-24 95.5
MsG0180000293.01.T02 AT1G47056 50.000 104 48 2 1 102 405 506 3.81e-20 84.3

Find 47 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACCTTCACTTTCACCAAAT+TGG 0.167556 1:+4062461 None:intergenic
AGTTGTCCTGTTTCTGATCT+TGG 0.260446 1:-4062510 MsG0180000293.01.T01:CDS
GCCGCGGAGCCGGGCATTGC+AGG 0.275184 1:+4062291 None:intergenic
GGCAAATGGGTGTCCCAATT+TGG 0.327661 1:-4062475 MsG0180000293.01.T01:CDS
ACCTTCACTTTCACCAAATT+GGG 0.329278 1:+4062462 None:intergenic
TGATCTTGGGATGGAAGCTT+TGG 0.344571 1:-4062496 MsG0180000293.01.T01:CDS
GTTGTCCTGTTTCTGATCTT+GGG 0.350129 1:-4062509 MsG0180000293.01.T01:CDS
TTGCATTGCTGCAAAGTGTT+TGG 0.356470 1:-4062556 MsG0180000293.01.T01:CDS
CTCAACCAATCCCCACCTTC+AGG 0.369729 1:+4062417 None:intergenic
AGTTCATTCAAGCAAAGGTT+AGG 0.388373 1:-4062225 MsG0180000293.01.T01:CDS
GGGATTGGTTGAGGCACACT+AGG 0.389313 1:-4062407 MsG0180000293.01.T01:CDS
AAAGCCAATCTCTCTAGATT+CGG 0.392075 1:+4062615 None:intergenic
GGGATGGAAGCTTTGGCAAA+TGG 0.408413 1:-4062489 MsG0180000293.01.T01:CDS
CGATGTAGATGCCGCGGAGC+CGG 0.410875 1:+4062281 None:intergenic
GGTTTCTGTAGCTGTGAATC+TGG 0.425023 1:-4062385 MsG0180000293.01.T01:CDS
GATGTAGATGCCGCGGAGCC+GGG 0.436914 1:+4062282 None:intergenic
TTACACCTGAAGGTGGGGAT+TGG 0.440333 1:-4062422 MsG0180000293.01.T01:CDS
TTGCCCGAATCTAGAGAGAT+TGG 0.454730 1:-4062619 MsG0180000293.01.T01:CDS
TCCTGTTTCTGATCTTGGGA+TGG 0.465939 1:-4062505 MsG0180000293.01.T01:CDS
GGATGGAAGCTTTGGCAAAT+GGG 0.471037 1:-4062488 MsG0180000293.01.T01:CDS
TCCATCCCAAGATCAGAAAC+AGG 0.475671 1:+4062504 None:intergenic
AGTGAAGGTCAAGAAGTGTA+AGG 0.477496 1:-4062448 MsG0180000293.01.T01:CDS
ATTGAATTTCCTGCAATGCC+CGG 0.481428 1:-4062300 MsG0180000293.01.T01:CDS
AAGCCAATCTCTCTAGATTC+GGG 0.481744 1:+4062616 None:intergenic
GATGGTGGAGCACAAGAGAA+TGG 0.489500 1:-4062324 MsG0180000293.01.T01:CDS
GATCGAGTTCATTCAAGCAA+AGG 0.510836 1:-4062230 MsG0180000293.01.T01:CDS
GCAGCAATGCAAGATATCTC+AGG 0.514016 1:+4062567 None:intergenic
CGGCTCCGCGGCATCTACAT+CGG 0.517144 1:-4062280 MsG0180000293.01.T01:CDS
ATGGTGGAGCACAAGAGAAT+GGG 0.527565 1:-4062323 MsG0180000293.01.T01:CDS
CTGAAGGTGGGGATTGGTTG+AGG 0.528050 1:-4062416 MsG0180000293.01.T01:CDS
TCCTGCAATGCCCGGCTCCG+CGG 0.534211 1:-4062292 MsG0180000293.01.T01:CDS
GGATTGGTTGAGGCACACTA+GGG 0.546523 1:-4062406 MsG0180000293.01.T01:CDS
GTTCATTCAAGCAAAGGTTA+GGG 0.551510 1:-4062224 MsG0180000293.01.T01:CDS
CAAAGGTTAGGGCTACTGTC+CGG 0.585207 1:-4062213 MsG0180000293.01.T01:CDS
AGGGTGTTACACCTGAAGGT+GGG 0.590987 1:-4062428 MsG0180000293.01.T01:CDS
GTGAAGGTCAAGAAGTGTAA+GGG 0.606922 1:-4062447 MsG0180000293.01.T01:CDS
TGTAAGGGTGTTACACCTGA+AGG 0.612165 1:-4062432 MsG0180000293.01.T01:CDS
GATGCTAGTGCTAGTGATGG+TGG 0.613432 1:-4062339 MsG0180000293.01.T01:CDS
TGTAGCTGTGAATCTGGACG+CGG 0.617383 1:-4062379 MsG0180000293.01.T01:CDS
TACTGTCCGGAGGAAAATTG+TGG 0.618876 1:-4062200 MsG0180000293.01.T01:CDS
GGGTGTTACACCTGAAGGTG+GGG 0.621074 1:-4062427 MsG0180000293.01.T01:CDS
TCCCAATTTGGTGAAAGTGA+AGG 0.632374 1:-4062463 MsG0180000293.01.T01:CDS
TTGTGTGCAAGTGATACTGT+TGG 0.633448 1:-4062594 MsG0180000293.01.T01:CDS
AAGGGTGTTACACCTGAAGG+TGG 0.641667 1:-4062429 MsG0180000293.01.T01:CDS
ATTTGCCGATGTAGATGCCG+CGG 0.660814 1:+4062275 None:intergenic
AGGTTAGGGCTACTGTCCGG+AGG 0.681434 1:-4062210 MsG0180000293.01.T01:CDS
CAAGATGCTAGTGCTAGTGA+TGG 0.682263 1:-4062342 MsG0180000293.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAAGCCAATCTCTCTAGATT+CGG + Chr1:4062209-4062228 None:intergenic 35.0%
ACCTTCACTTTCACCAAATT+GGG + Chr1:4062362-4062381 None:intergenic 35.0%
AGTTCATTCAAGCAAAGGTT+AGG - Chr1:4062596-4062615 MsG0180000293.01.T01:CDS 35.0%
GTTCATTCAAGCAAAGGTTA+GGG - Chr1:4062597-4062616 MsG0180000293.01.T01:CDS 35.0%
AAGCCAATCTCTCTAGATTC+GGG + Chr1:4062208-4062227 None:intergenic 40.0%
ATTGAATTTCCTGCAATGCC+CGG - Chr1:4062521-4062540 MsG0180000293.01.T01:CDS 40.0%
GACCTTCACTTTCACCAAAT+TGG + Chr1:4062363-4062382 None:intergenic 40.0%
GATCGAGTTCATTCAAGCAA+AGG - Chr1:4062591-4062610 MsG0180000293.01.T01:CDS 40.0%
TTGTGTGCAAGTGATACTGT+TGG - Chr1:4062227-4062246 MsG0180000293.01.T01:CDS 40.0%
! AGTGAAGGTCAAGAAGTGTA+AGG - Chr1:4062373-4062392 MsG0180000293.01.T01:CDS 40.0%
! GTGAAGGTCAAGAAGTGTAA+GGG - Chr1:4062374-4062393 MsG0180000293.01.T01:CDS 40.0%
! TCCCAATTTGGTGAAAGTGA+AGG - Chr1:4062358-4062377 MsG0180000293.01.T01:CDS 40.0%
! TTGCATTGCTGCAAAGTGTT+TGG - Chr1:4062265-4062284 MsG0180000293.01.T01:CDS 40.0%
!! AGTTGTCCTGTTTCTGATCT+TGG - Chr1:4062311-4062330 MsG0180000293.01.T01:CDS 40.0%
!! GTTGTCCTGTTTCTGATCTT+GGG - Chr1:4062312-4062331 MsG0180000293.01.T01:CDS 40.0%
CAAGATGCTAGTGCTAGTGA+TGG - Chr1:4062479-4062498 MsG0180000293.01.T01:CDS 45.0%
GCAGCAATGCAAGATATCTC+AGG + Chr1:4062257-4062276 None:intergenic 45.0%
GGTTTCTGTAGCTGTGAATC+TGG - Chr1:4062436-4062455 MsG0180000293.01.T01:CDS 45.0%
TACTGTCCGGAGGAAAATTG+TGG - Chr1:4062621-4062640 MsG0180000293.01.T01:CDS 45.0%
TCCATCCCAAGATCAGAAAC+AGG + Chr1:4062320-4062339 None:intergenic 45.0%
TGATCTTGGGATGGAAGCTT+TGG - Chr1:4062325-4062344 MsG0180000293.01.T01:CDS 45.0%
TTGCCCGAATCTAGAGAGAT+TGG - Chr1:4062202-4062221 MsG0180000293.01.T01:CDS 45.0%
! ATGGTGGAGCACAAGAGAAT+GGG - Chr1:4062498-4062517 MsG0180000293.01.T01:CDS 45.0%
! GGATGGAAGCTTTGGCAAAT+GGG - Chr1:4062333-4062352 MsG0180000293.01.T01:CDS 45.0%
! TGCTAGCCACAATTTTCCTC+CGG + Chr1:4062630-4062649 None:intergenic 45.0%
!! TCCTGTTTCTGATCTTGGGA+TGG - Chr1:4062316-4062335 MsG0180000293.01.T01:CDS 45.0%
!! TGTAAGGGTGTTACACCTGA+AGG - Chr1:4062389-4062408 MsG0180000293.01.T01:CDS 45.0%
ATTTGCCGATGTAGATGCCG+CGG + Chr1:4062549-4062568 None:intergenic 50.0%
CAAAGGTTAGGGCTACTGTC+CGG - Chr1:4062608-4062627 MsG0180000293.01.T01:CDS 50.0%
GATGCTAGTGCTAGTGATGG+TGG - Chr1:4062482-4062501 MsG0180000293.01.T01:CDS 50.0%
GGCAAATGGGTGTCCCAATT+TGG - Chr1:4062346-4062365 MsG0180000293.01.T01:CDS 50.0%
TGTAGCTGTGAATCTGGACG+CGG - Chr1:4062442-4062461 MsG0180000293.01.T01:CDS 50.0%
TTACACCTGAAGGTGGGGAT+TGG - Chr1:4062399-4062418 MsG0180000293.01.T01:CDS 50.0%
! AGGGTGTTACACCTGAAGGT+GGG - Chr1:4062393-4062412 MsG0180000293.01.T01:CDS 50.0%
! GGGATGGAAGCTTTGGCAAA+TGG - Chr1:4062332-4062351 MsG0180000293.01.T01:CDS 50.0%
!! AAGGGTGTTACACCTGAAGG+TGG - Chr1:4062392-4062411 MsG0180000293.01.T01:CDS 50.0%
!! GATGGTGGAGCACAAGAGAA+TGG - Chr1:4062497-4062516 MsG0180000293.01.T01:CDS 50.0%
!! GGATTGGTTGAGGCACACTA+GGG - Chr1:4062415-4062434 MsG0180000293.01.T01:CDS 50.0%
CTCAACCAATCCCCACCTTC+AGG + Chr1:4062407-4062426 None:intergenic 55.0%
! GGGTGTTACACCTGAAGGTG+GGG - Chr1:4062394-4062413 MsG0180000293.01.T01:CDS 55.0%
!! CTGAAGGTGGGGATTGGTTG+AGG - Chr1:4062405-4062424 MsG0180000293.01.T01:CDS 55.0%
!! GGGATTGGTTGAGGCACACT+AGG - Chr1:4062414-4062433 MsG0180000293.01.T01:CDS 55.0%
AGGTTAGGGCTACTGTCCGG+AGG - Chr1:4062611-4062630 MsG0180000293.01.T01:CDS 60.0%
CGATGTAGATGCCGCGGAGC+CGG + Chr1:4062543-4062562 None:intergenic 65.0%
CGGCTCCGCGGCATCTACAT+CGG - Chr1:4062541-4062560 MsG0180000293.01.T01:CDS 65.0%
GATGTAGATGCCGCGGAGCC+GGG + Chr1:4062542-4062561 None:intergenic 65.0%
TCCTGCAATGCCCGGCTCCG+CGG - Chr1:4062529-4062548 MsG0180000293.01.T01:CDS 70.0%
!! GCCGCGGAGCCGGGCATTGC+AGG + Chr1:4062533-4062552 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
Chr1 gene 4062188 4062655 4062188 ID=MsG0180000293.01;Name=MsG0180000293.01
Chr1 mRNA 4062188 4062655 4062188 ID=MsG0180000293.01.T01;Parent=MsG0180000293.01;Name=MsG0180000293.01.T01;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|0|1|0|155
Chr1 exon 4062188 4062655 4062188 ID=MsG0180000293.01.T01:exon:5753;Parent=MsG0180000293.01.T01
Chr1 CDS 4062188 4062655 4062188 ID=MsG0180000293.01.T01:cds;Parent=MsG0180000293.01.T01
Chr1 mRNA 4062188 4062508 4062188 ID=MsG0180000293.01.T02;Parent=MsG0180000293.01;Name=MsG0180000293.01.T02;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|0|1|0|106
Chr1 exon 4062188 4062508 4062188 ID=MsG0180000293.01.T02:exon:5754;Parent=MsG0180000293.01.T02
Chr1 CDS 4062188 4062508 4062188 ID=MsG0180000293.01.T02:cds;Parent=MsG0180000293.01.T02
Gene Sequence

>MsG0180000293.01.T01

ATGTTGGCATCGAATTGCCCGAATCTAGAGAGATTGGCTTTGTGTGCAAGTGATACTGTTGGTGATCCTGAGATATCTTGCATTGCTGCAAAGTGTTTGGCTTTGAAGAAACTTTGCATCAAGAGTTGTCCTGTTTCTGATCTTGGGATGGAAGCTTTGGCAAATGGGTGTCCCAATTTGGTGAAAGTGAAGGTCAAGAAGTGTAAGGGTGTTACACCTGAAGGTGGGGATTGGTTGAGGCACACTAGGGTTTCTGTAGCTGTGAATCTGGACGCGGTTGAAGCAGAACTTCAAGATGCTAGTGCTAGTGATGGTGGAGCACAAGAGAATGGGATTGAATTTCCTGCAATGCCCGGCTCCGCGGCATCTACATCGGCAAATATTGCATCGAGAAGTACTGTTCGATCGAGTTCATTCAAGCAAAGGTTAGGGCTACTGTCCGGAGGAAAATTGTGGCTAGCACTTTGA

>MsG0180000293.01.T02

ATGGAAGCTTTGGCAAATGGGTGTCCCAATTTGGTGAAAGTGAAGGTCAAGAAGTGTAAGGGTGTTACACCTGAAGGTGGGGATTGGTTGAGGCACACTAGGGTTTCTGTAGCTGTGAATCTGGACGCGGTTGAAGCAGAACTTCAAGATGCTAGTGCTAGTGATGGTGGAGCACAAGAGAATGGGATTGAATTTCCTGCAATGCCCGGCTCCGCGGCATCTACATCGGCAAATATTGCATCGAGAAGTACTGTTCGATCGAGTTCATTCAAGCAAAGGTTAGGGCTACTGTCCGGAGGAAAATTGTGGCTAGCACTTTGA

Protein sequence

>MsG0180000293.01.T01

MLASNCPNLERLALCASDTVGDPEISCIAAKCLALKKLCIKSCPVSDLGMEALANGCPNLVKVKVKKCKGVTPEGGDWLRHTRVSVAVNLDAVEAELQDASASDGGAQENGIEFPAMPGSAASTSANIASRSTVRSSSFKQRLGLLSGGKLWLAL*

>MsG0180000293.01.T02

MEALANGCPNLVKVKVKKCKGVTPEGGDWLRHTRVSVAVNLDAVEAELQDASASDGGAQENGIEFPAMPGSAASTSANIASRSTVRSSSFKQRLGLLSGGKLWLAL*