AlfalfaGEDB | MsG0480021447.01

M. sativa cultivar ZhongmuNo.1 / MsG0480021447.01

KEGG: K02901
GO: GO:0003735 GO:0005622 GO:0005840 GO:0006412
PFAM: PF00467 PF01777

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021447.01.T01	XP_003594893.2	97.778	135	3	0	1	135	1	135	9.06E-89	264

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021447.01.T01	Q05462	87.407	135	17	0	1	135	1	135	4.38E-82	239

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048113.01	MsG0480021447.01	0.803841	2.875899e-49	1.460286e-46
MsG0080048638.01	MsG0480021447.01	0.811495	6.804514e-51	4.218560e-48
MsG0080048856.01	MsG0480021447.01	0.812459	4.194696e-51	2.668689e-48
MsG0180005250.01	MsG0480021447.01	0.824676	7.095086e-54	6.328620e-51
MsG0280010698.01	MsG0480021447.01	0.804248	2.366455e-49	1.214141e-46
MsG0380014537.01	MsG0480021447.01	0.815395	9.449639e-52	6.507393e-49
MsG0480021447.01	MsG0480023463.01	0.806995	6.275235e-50	3.455072e-47
MsG0480021447.01	MsG0680031214.01	0.801116	1.047805e-48	4.963653e-46
MsG0480021447.01	MsG0680031488.01	0.801802	7.583143e-49	3.654884e-46
MsG0480021447.01	MsG0780038401.01	0.815382	9.515264e-52	6.550236e-49
MsG0480021447.01	MsG0780040704.01	0.803779	2.961375e-49	1.501275e-46
MsG0380015168.01	MsG0480021447.01	0.813491	2.491747e-51	1.629663e-48
MsG0380016326.01	MsG0480021447.01	0.805293	1.431572e-49	7.545652e-47

PPI

Gene1	Gene2	Type
MsG0480021447.01	MsG0280008118.01	PPI
MsG0180001372.01	MsG0480021447.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021447.01.T01	MTR_2g035930	97.778	135	3	0	1	135	1	135	1.10e-92	264
MsG0480021447.01.T01	MTR_2g006170	97.037	135	4	0	1	135	1	135	3.55e-92	263
MsG0480021447.01.T01	MTR_3g048580	60.870	69	26	1	67	135	29	96	7.72e-19	75.9

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021447.01.T01	AT3G22230	85.926	135	19	1	135	1	135	6.92e-83	239
MsG0480021447.01.T01	AT4G15000	85.185	135	20	1	135	1	135	9.74e-82	236
MsG0480021447.01.T01	AT2G32220	80.000	135	27	1	135	1	135	2.90e-77	225
MsG0480021447.01.T01	AT4G15000	83.740	123	20	1	123	1	123	5.55e-71	209

Find 39 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACGATGACGGCTTTCTTCC+CGG	0.266318	4:-57955950	None:intergenic
TACGGCCATTGTCTTGTTGC+AGG	0.322088	4:+57956006	MsG0480021447.01.T01:CDS
CACACTTGATGTTGATTTGA+AGG	0.351053	4:+57956149	MsG0480021447.01.T01:CDS
AGGACGATGACGGCCTTGTT+TGG	0.382413	4:-57955913	None:intergenic
GGTTTAAGACTGGGAAGAAT+AGG	0.401208	4:+57956253	MsG0480021447.01.T01:CDS
TCCTTCTTGATAACTTTAGC+TGG	0.410794	4:-57956042	None:intergenic
TACTGATGTTCTTCAGAGTA+AGG	0.417538	4:+57956182	MsG0480021447.01.T01:CDS
TCCCGGCGTAGCGTCCTTGA+AGG	0.429146	4:-57955933	None:intergenic
TAAGAAGGTGACTGCTTTGA+AGG	0.432710	4:+57956206	MsG0480021447.01.T01:CDS
TCCTTCAAGGACGCTACGCC+GGG	0.449272	4:+57955932	MsG0480021447.01.T01:CDS
CTTCACACGCGATTTCTTCG+CGG	0.465894	4:-57956079	None:intergenic
GGTGGTTCTTTACCAAGCTT+AGG	0.481134	4:+57956274	MsG0480021447.01.T01:CDS
GTCGAAATTCTTCACGATGA+CGG	0.503904	4:-57955962	None:intergenic
TTCGAGGAGAGGTTTAAGAC+TGG	0.506310	4:+57956243	MsG0480021447.01.T01:CDS
AAGGCCGTCATCGTCCTTCA+AGG	0.506838	4:+57955919	MsG0480021447.01.T01:CDS
CGCGAAGAAATCGCGTGTGA+AGG	0.515996	4:+57956080	MsG0480021447.01.T01:CDS
AACAGCTCAAAACCTAAGCT+TGG	0.523953	4:-57956286	None:intergenic
GGAACCTGTGACCGTGCATA+CGG	0.527101	4:+57955988	MsG0480021447.01.T01:CDS
GCGAAGAAATCGCGTGTGAA+GGG	0.527543	4:+57956081	MsG0480021447.01.T01:CDS
CGAAGAAGAGGTTCGAGGAG+AGG	0.532977	4:+57956232	MsG0480021447.01.T01:CDS
AACGCGTCGGCATCATATGC+TGG	0.536923	4:-57956125	None:intergenic
TCGAGGAGAGGTTTAAGACT+GGG	0.540939	4:+57956244	MsG0480021447.01.T01:CDS
ACGGCCATTGTCTTGTTGCA+GGG	0.554142	4:+57956007	MsG0480021447.01.T01:CDS
GTCCTTCAAGGACGCTACGC+CGG	0.559359	4:+57955931	MsG0480021447.01.T01:CDS
TCTTCAGAGTAAGGATAAGA+AGG	0.568239	4:+57956191	MsG0480021447.01.T01:CDS
TCCAGCTAAAGTTATCAAGA+AGG	0.575607	4:+57956041	MsG0480021447.01.T01:CDS
CAAGTTTCTGAAGCCAAACA+AGG	0.581514	4:+57955900	MsG0480021447.01.T01:CDS
ATGGCCGTATGCACGGTCAC+AGG	0.582267	4:-57955992	None:intergenic
CAAGACAATGGCCGTATGCA+CGG	0.597016	4:-57955999	None:intergenic
CTTTGAAGGAGGCGAAGAAG+AGG	0.602084	4:+57956220	MsG0480021447.01.T01:CDS
CGAAGAAATCGCGTGTGAAG+GGG	0.603203	4:+57956082	MsG0480021447.01.T01:CDS
GGAGGCGAAGAAGAGGTTCG+AGG	0.607573	4:+57956227	MsG0480021447.01.T01:CDS
TTAAGACTGGGAAGAATAGG+TGG	0.614047	4:+57956256	MsG0480021447.01.T01:CDS
GCGTCCTTGAAGGACGATGA+CGG	0.637453	4:-57955923	None:intergenic
TGATCCCTGCAACAAGACAA+TGG	0.637766	4:-57956011	None:intergenic
GAAGGTGACTGCTTTGAAGG+AGG	0.640915	4:+57956209	MsG0480021447.01.T01:CDS
ACATCAAGTGTGTAACGCGT+CGG	0.653766	4:-57956138	None:intergenic
ACTTGATGTTGATTTGAAGG+AGG	0.687643	4:+57956152	MsG0480021447.01.T01:CDS
ATCGTGAAGAATTTCGACGA+CGG	0.729202	4:+57955967	MsG0480021447.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TACTGATGTTCTTCAGAGTA+AGG	+	Chr4:57956182-57956201	MsG0480021447.01.T01:CDS	35.0%
	TCCAGCTAAAGTTATCAAGA+AGG	+	Chr4:57956041-57956060	MsG0480021447.01.T01:CDS	35.0%
	TCTTCAGAGTAAGGATAAGA+AGG	+	Chr4:57956191-57956210	MsG0480021447.01.T01:CDS	35.0%
!	TCCTTCTTGATAACTTTAGC+TGG	-	Chr4:57956045-57956064	None:intergenic	35.0%
!!	ACTTGATGTTGATTTGAAGG+AGG	+	Chr4:57956152-57956171	MsG0480021447.01.T01:CDS	35.0%
!!	CACACTTGATGTTGATTTGA+AGG	+	Chr4:57956149-57956168	MsG0480021447.01.T01:CDS	35.0%
	ATCGTGAAGAATTTCGACGA+CGG	+	Chr4:57955967-57955986	MsG0480021447.01.T01:CDS	40.0%
	GTCGAAATTCTTCACGATGA+CGG	-	Chr4:57955965-57955984	None:intergenic	40.0%
	TAAGAAGGTGACTGCTTTGA+AGG	+	Chr4:57956206-57956225	MsG0480021447.01.T01:CDS	40.0%
	TTAAGACTGGGAAGAATAGG+TGG	+	Chr4:57956256-57956275	MsG0480021447.01.T01:CDS	40.0%
!	GGTTTAAGACTGGGAAGAAT+AGG	+	Chr4:57956253-57956272	MsG0480021447.01.T01:CDS	40.0%
!!	CAAGTTTCTGAAGCCAAACA+AGG	+	Chr4:57955900-57955919	MsG0480021447.01.T01:CDS	40.0%
	ACATCAAGTGTGTAACGCGT+CGG	-	Chr4:57956141-57956160	None:intergenic	45.0%
	GGTGGTTCTTTACCAAGCTT+AGG	+	Chr4:57956274-57956293	MsG0480021447.01.T01:CDS	45.0%
	TGATCCCTGCAACAAGACAA+TGG	-	Chr4:57956014-57956033	None:intergenic	45.0%
!	TCGAGGAGAGGTTTAAGACT+GGG	+	Chr4:57956244-57956263	MsG0480021447.01.T01:CDS	45.0%
!	TTCGAGGAGAGGTTTAAGAC+TGG	+	Chr4:57956243-57956262	MsG0480021447.01.T01:CDS	45.0%
	CAAGACAATGGCCGTATGCA+CGG	-	Chr4:57956002-57956021	None:intergenic	50.0%
	CGAAGAAATCGCGTGTGAAG+GGG	+	Chr4:57956082-57956101	MsG0480021447.01.T01:CDS	50.0%
	CTTCACACGCGATTTCTTCG+CGG	-	Chr4:57956082-57956101	None:intergenic	50.0%
	GCGAAGAAATCGCGTGTGAA+GGG	+	Chr4:57956081-57956100	MsG0480021447.01.T01:CDS	50.0%
!	ACGGCCATTGTCTTGTTGCA+GGG	+	Chr4:57956007-57956026	MsG0480021447.01.T01:CDS	50.0%
!	CTTTGAAGGAGGCGAAGAAG+AGG	+	Chr4:57956220-57956239	MsG0480021447.01.T01:CDS	50.0%
!	TACGGCCATTGTCTTGTTGC+AGG	+	Chr4:57956006-57956025	MsG0480021447.01.T01:CDS	50.0%
!!	GAAGGTGACTGCTTTGAAGG+AGG	+	Chr4:57956209-57956228	MsG0480021447.01.T01:CDS	50.0%
	AAGGCCGTCATCGTCCTTCA+AGG	+	Chr4:57955919-57955938	MsG0480021447.01.T01:CDS	55.0%
	CGAAGAAGAGGTTCGAGGAG+AGG	+	Chr4:57956232-57956251	MsG0480021447.01.T01:CDS	55.0%
	CGCGAAGAAATCGCGTGTGA+AGG	+	Chr4:57956080-57956099	MsG0480021447.01.T01:CDS	55.0%
	GCGTCCTTGAAGGACGATGA+CGG	-	Chr4:57955926-57955945	None:intergenic	55.0%
	GGAACCTGTGACCGTGCATA+CGG	+	Chr4:57955988-57956007	MsG0480021447.01.T01:CDS	55.0%
!	AACGCGTCGGCATCATATGC+TGG	-	Chr4:57956128-57956147	None:intergenic	55.0%
!	CACGATGACGGCTTTCTTCC+CGG	-	Chr4:57955953-57955972	None:intergenic	55.0%
!!	AGGACGATGACGGCCTTGTT+TGG	-	Chr4:57955916-57955935	None:intergenic	55.0%
	ATGGCCGTATGCACGGTCAC+AGG	-	Chr4:57955995-57956014	None:intergenic	60.0%
	GGAGGCGAAGAAGAGGTTCG+AGG	+	Chr4:57956227-57956246	MsG0480021447.01.T01:CDS	60.0%
	GTCCTTCAAGGACGCTACGC+CGG	+	Chr4:57955931-57955950	MsG0480021447.01.T01:CDS	60.0%
	TCCTTCAAGGACGCTACGCC+GGG	+	Chr4:57955932-57955951	MsG0480021447.01.T01:CDS	60.0%
	TCCCGGCGTAGCGTCCTTGA+AGG	-	Chr4:57955936-57955955	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	57955895	57956302	57955895	ID=MsG0480021447.01;Name=MsG0480021447.01
Chr4	mRNA	57955895	57956302	57955895	ID=MsG0480021447.01.T01;Parent=MsG0480021447.01;Name=MsG0480021447.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|135
Chr4	exon	57955895	57956302	57955895	ID=MsG0480021447.01.T01:exon:17224;Parent=MsG0480021447.01.T01
Chr4	CDS	57955895	57956302	57955895	ID=MsG0480021447.01.T01:cds;Parent=MsG0480021447.01.T01

Gene Sequence

>MsG0480021447.01.T01

ATGGTCAAGTTTCTGAAGCCAAACAAGGCCGTCATCGTCCTTCAAGGACGCTACGCCGGGAAGAAAGCCGTCATCGTGAAGAATTTCGACGACGGAACCTGTGACCGTGCATACGGCCATTGTCTTGTTGCAGGGATCAAGAAGTATCCAGCTAAAGTTATCAAGAAGGATTCAGCGAAGAAAACCGCGAAGAAATCGCGTGTGAAGGGGTTCGTCAAGCTTGTTAACTACCAGCATATGATGCCGACGCGTTACACACTTGATGTTGATTTGAAGGAGGTTGTTACTACTGATGTTCTTCAGAGTAAGGATAAGAAGGTGACTGCTTTGAAGGAGGCGAAGAAGAGGTTCGAGGAGAGGTTTAAGACTGGGAAGAATAGGTGGTTCTTTACCAAGCTTAGGTTTTGA

Protein sequence

>MsG0480021447.01.T01

MVKFLKPNKAVIVLQGRYAGKKAVIVKNFDDGTCDRAYGHCLVAGIKKYPAKVIKKDSAKKTAKKSRVKGFVKLVNYQHMMPTRYTLDVDLKEVVTTDVLQSKDKKVTALKEAKKRFEERFKTGKNRWFFTKLRF*