AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380011617.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380011617.01.T01 MTR_3g007250 70.548 146 6 1 1 109 1 146 2.11e-63 189
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380011617.01.T01 AT3G22480 44.667 150 41 4 1 110 1 148 1.25e-27 99.0
MsG0380011617.01.T01 AT3G22480 44.667 150 41 4 1 110 1 148 1.25e-27 99.0

Find 30 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTTCAAGCTCCGTAATTT+TGG 0.220078 3:-2472719 None:intergenic
GCAAGTGCTTGTTCATTTAC+TGG 0.240244 3:-2472652 None:intergenic
CTGCTGTGCAGCGAAATAAA+AGG 0.380079 3:+2472857 MsG0380011617.01.T01:intron
CAAAATTACGGAGCTTGAAA+TGG 0.381749 3:+2472720 MsG0380011617.01.T01:CDS
GGAGTACTTGTGGGCCCTGC+AGG 0.391963 3:+2473028 MsG0380011617.01.T01:CDS
CCAAATTTACACCAAAATTA+CGG 0.395846 3:+2472708 MsG0380011617.01.T01:CDS
CAGCATCGGCCTTCCTCATC+CGG 0.415801 3:-2472964 None:intergenic
AGACATTGGAAAAGAAGAAA+AGG 0.423618 3:+2472909 MsG0380011617.01.T01:CDS
TGTCATTTGCTACCACCTGC+AGG 0.484824 3:-2473043 None:intergenic
TTGAGACTAAATATAAAATC+CGG 0.490885 3:+2472945 MsG0380011617.01.T01:CDS
TCTGCTCAAGGAGTACTTGT+GGG 0.504138 3:+2473019 MsG0380011617.01.T01:CDS
GTGTAGAATCATGGCTAGTA+AGG 0.508954 3:+2472612 None:intergenic
GACATTGGAAAAGAAGAAAA+GGG 0.510315 3:+2472910 MsG0380011617.01.T01:CDS
GCTGAGGTGAACGATGAATC+TGG 0.515292 3:+2472983 MsG0380011617.01.T01:CDS
TGCAAGGCTTAACAAGACAT+TGG 0.518757 3:+2472895 MsG0380011617.01.T01:CDS
GTACTTGTGGGCCCTGCAGG+TGG 0.539955 3:+2473031 MsG0380011617.01.T01:CDS
GAACGATGAATCTGGTAAGA+AGG 0.544140 3:+2472991 MsG0380011617.01.T01:CDS
GATGAGGAAGGCCGATGCTG+AGG 0.574674 3:+2472967 MsG0380011617.01.T01:CDS
AAGAAGGAAGGTTCTGCTCA+AGG 0.575142 3:+2473007 MsG0380011617.01.T01:CDS
TTCATCGTTCACCTCAGCAT+CGG 0.575473 3:-2472978 None:intergenic
CAAATATATATGCAGCTATG+AGG 0.582009 3:+2472674 MsG0380011617.01.T01:CDS
CGAAATAAAAGGACTTGAAG+AGG 0.583338 3:+2472868 MsG0380011617.01.T01:CDS
ATATAAAATCCGGATGAGGA+AGG 0.583660 3:+2472955 MsG0380011617.01.T01:CDS
TTCTGCTCAAGGAGTACTTG+TGG 0.584158 3:+2473018 MsG0380011617.01.T01:CDS
GATGAATCTGGTAAGAAGGA+AGG 0.587326 3:+2472995 MsG0380011617.01.T01:CDS
CTAAATATAAAATCCGGATG+AGG 0.587775 3:+2472951 MsG0380011617.01.T01:CDS
GACTTGAAGAGGTTGTTGCA+AGG 0.593695 3:+2472879 MsG0380011617.01.T01:CDS
GTCATTTGCTACCACCTGCA+GGG 0.594429 3:-2473042 None:intergenic
TAGTAAGGCGGAAGATCGCA+AGG 0.597879 3:+2472627 MsG0380011617.01.T01:CDS
TAGAATCATGGCTAGTAAGG+CGG 0.646340 3:+2472615 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGAGACTAAATATAAAATC+CGG + Chr3:2472945-2472964 MsG0380011617.01.T01:CDS 20.0%
! CCAAATTTACACCAAAATTA+CGG + Chr3:2472708-2472727 MsG0380011617.01.T01:CDS 25.0%
AGACATTGGAAAAGAAGAAA+AGG + Chr3:2472909-2472928 MsG0380011617.01.T01:CDS 30.0%
CAAATATATATGCAGCTATG+AGG + Chr3:2472674-2472693 MsG0380011617.01.T01:CDS 30.0%
CTAAATATAAAATCCGGATG+AGG + Chr3:2472951-2472970 MsG0380011617.01.T01:CDS 30.0%
GACATTGGAAAAGAAGAAAA+GGG + Chr3:2472910-2472929 MsG0380011617.01.T01:CDS 30.0%
!! CCGTAATTTTGGTGTAAATT+TGG - Chr3:2472711-2472730 None:intergenic 30.0%
!! TTTTGGTGTAAATTTGGTTG+AGG - Chr3:2472705-2472724 None:intergenic 30.0%
ATATAAAATCCGGATGAGGA+AGG + Chr3:2472955-2472974 MsG0380011617.01.T01:CDS 35.0%
CAAAATTACGGAGCTTGAAA+TGG + Chr3:2472720-2472739 MsG0380011617.01.T01:CDS 35.0%
CGAAATAAAAGGACTTGAAG+AGG + Chr3:2472868-2472887 MsG0380011617.01.T01:CDS 35.0%
! CATTTCAAGCTCCGTAATTT+TGG - Chr3:2472722-2472741 None:intergenic 35.0%
AGACGATGCTATCGAATGAT+CGG + Chr3:2472796-2472815 MsG0380011617.01.T01:intron 40.0%
GAACGATGAATCTGGTAAGA+AGG + Chr3:2472991-2473010 MsG0380011617.01.T01:CDS 40.0%
GATGAATCTGGTAAGAAGGA+AGG + Chr3:2472995-2473014 MsG0380011617.01.T01:CDS 40.0%
GCAAGTGCTTGTTCATTTAC+TGG - Chr3:2472655-2472674 None:intergenic 40.0%
TCTTAATGCTATTCAGCTGC+TGG + Chr3:2472765-2472784 MsG0380011617.01.T01:intron 40.0%
TGCAAGGCTTAACAAGACAT+TGG + Chr3:2472895-2472914 MsG0380011617.01.T01:CDS 40.0%
TGTGGAAAGAACTGTCAAAG+AGG + Chr3:2472828-2472847 MsG0380011617.01.T01:intron 40.0%
TTCGATAGCATCGTCTAGAT+TGG - Chr3:2472792-2472811 None:intergenic 40.0%
!! TTTTATTTCGCTGCACAGCA+GGG - Chr3:2472858-2472877 None:intergenic 40.0%
AAGAAGGAAGGTTCTGCTCA+AGG + Chr3:2473007-2473026 MsG0380011617.01.T01:CDS 45.0%
ATGCTATCGAATGATCGGAG+GGG + Chr3:2472801-2472820 MsG0380011617.01.T01:intron 45.0%
CTGCTGTGCAGCGAAATAAA+AGG + Chr3:2472857-2472876 MsG0380011617.01.T01:intron 45.0%
GACTTGAAGAGGTTGTTGCA+AGG + Chr3:2472879-2472898 MsG0380011617.01.T01:CDS 45.0%
GATGCTATCGAATGATCGGA+GGG + Chr3:2472800-2472819 MsG0380011617.01.T01:intron 45.0%
TTCATCGTTCACCTCAGCAT+CGG - Chr3:2472981-2473000 None:intergenic 45.0%
!! CTTTTATTTCGCTGCACAGC+AGG - Chr3:2472859-2472878 None:intergenic 45.0%
!! TCTGCTCAAGGAGTACTTGT+GGG + Chr3:2473019-2473038 MsG0380011617.01.T01:CDS 45.0%
!! TTCTGCTCAAGGAGTACTTG+TGG + Chr3:2473018-2473037 MsG0380011617.01.T01:CDS 45.0%
CGATGCTATCGAATGATCGG+AGG + Chr3:2472799-2472818 MsG0380011617.01.T01:intron 50.0%
GCTGAGGTGAACGATGAATC+TGG + Chr3:2472983-2473002 MsG0380011617.01.T01:CDS 50.0%
TAGTAAGGCGGAAGATCGCA+AGG + Chr3:2472627-2472646 MsG0380011617.01.T01:CDS 50.0%
!! AATGATCGGAGGGGTGCTTG+TGG + Chr3:2472810-2472829 MsG0380011617.01.T01:intron 55.0%
GATGAGGAAGGCCGATGCTG+AGG + Chr3:2472967-2472986 MsG0380011617.01.T01:CDS 60.0%
GGAAGTCAGCGAGCACTCGT+TGG + Chr3:2472741-2472760 MsG0380011617.01.T01:intron 60.0%
!! CAGCATCGGCCTTCCTCATC+CGG - Chr3:2472967-2472986 None:intergenic 60.0%
GTACTTGTGGGCCCTGCAGG+TGG + Chr3:2473031-2473050 MsG0380011617.01.T01:CDS 65.0%
! GGAGTACTTGTGGGCCCTGC+AGG + Chr3:2473028-2473047 MsG0380011617.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr3 gene 2472622 2473063 2472622 ID=MsG0380011617.01;Name=MsG0380011617.01
Chr3 mRNA 2472622 2473063 2472622 ID=MsG0380011617.01.T01;Parent=MsG0380011617.01;Name=MsG0380011617.01.T01;_AED=0.50;_eAED=0.51;_QI=0|0|0|1|1|1|2|0|110
Chr3 exon 2472622 2472758 2472622 ID=MsG0380011617.01.T01:exon:805;Parent=MsG0380011617.01.T01
Chr3 exon 2472868 2473063 2472868 ID=MsG0380011617.01.T01:exon:806;Parent=MsG0380011617.01.T01
Chr3 CDS 2472622 2472758 2472622 ID=MsG0380011617.01.T01:cds;Parent=MsG0380011617.01.T01
Chr3 CDS 2472868 2473063 2472868 ID=MsG0380011617.01.T01:cds;Parent=MsG0380011617.01.T01
Gene Sequence

>MsG0380011617.01.T01

ATGGCTAGTAAGGCGGAAGATCGCAAGGAGCCAGTAAATGAACAAGCACTTGCAAATATATATGCAGCTATGAGGTCAGACCTCAACCAAATTTACACCAAAATTACGGAGCTTGAAATGGAAGTCAGCGAGCACTCCGAAATAAAAGGACTTGAAGAGGTTGTTGCAAGGCTTAACAAGACATTGGAAAAGAAGAAAAGGGAAATTGCTGAGTTTGAGACTAAATATAAAATCCGGATGAGGAAGGCCGATGCTGAGGTGAACGATGAATCTGGTAAGAAGGAAGGTTCTGCTCAAGGAGTACTTGTGGGCCCTGCAGGTGGTAGCAAATGA

Protein sequence

>MsG0380011617.01.T01

MASKAEDRKEPVNEQALANIYAAMRSDLNQIYTKITELEMEVSEHSEIKGLEEVVARLNKTLEKKKREIAEFETKYKIRMRKADAEVNDESGKKEGSAQGVLVGPAGGSK*