AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018311.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018311.01.T01 MTR_4g009740 96.581 117 4 0 1 117 1 117 1.41e-74 241
MsG0480018311.01.T01 MTR_4g009710 85.965 114 16 0 1 114 1 114 7.30e-68 207
MsG0480018311.01.T01 MTR_4g009710 82.292 96 17 0 1 96 120 215 1.18e-52 169
MsG0480018311.01.T01 MTR_4g009630 75.410 122 29 1 7 127 201 322 1.23e-59 187
MsG0480018311.01.T01 MTR_4g009630 81.481 108 20 0 7 114 201 308 2.00e-58 186
MsG0480018311.01.T01 MTR_2g104400 73.171 123 33 0 1 123 1 123 9.72e-57 190
MsG0480018311.01.T01 MTR_2g095920 76.786 112 26 0 1 112 1 112 4.02e-56 182
MsG0480018311.01.T01 MTR_2g104430 75.893 112 27 0 1 112 42 153 3.87e-54 179
MsG0480018311.01.T01 MTR_2g104430 71.429 112 26 1 1 112 1 106 7.12e-48 161
MsG0480018311.01.T01 MTR_2g104430 67.857 112 26 1 1 112 42 143 3.82e-44 152
MsG0480018311.01.T01 MTR_2g104400 73.256 86 23 0 38 123 1 86 3.17e-36 131
MsG0480018311.01.T01 MTR_2g104460 50.000 84 35 1 17 100 15 91 3.83e-20 79.3
MsG0480018311.01.T01 MTR_2g104420 61.538 52 20 0 9 60 139 190 4.31e-14 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018311.01.T01 AT1G15780 59.167 120 34 3 1 105 1 120 1.35e-38 138
MsG0480018311.01.T01 AT1G15780 59.167 120 34 3 1 105 1 120 1.42e-38 138
MsG0480018311.01.T01 AT1G15780 59.167 120 34 3 1 105 23 142 1.57e-38 138
MsG0480018311.01.T01 AT1G15790 58.974 78 32 0 18 95 4 81 3.05e-24 92.4
MsG0480018311.01.T01 AT1G15790 50.000 82 39 1 7 88 100 179 5.01e-19 79.0
MsG0480018311.01.T01 AT1G15790 58.974 78 32 0 18 95 4 81 3.05e-24 92.4
MsG0480018311.01.T01 AT1G15790 50.000 82 39 1 7 88 100 179 5.01e-19 79.0
MsG0480018311.01.T01 AT1G15790 58.974 78 32 0 18 95 4 81 3.05e-24 92.4
MsG0480018311.01.T01 AT1G15790 50.000 82 39 1 7 88 100 179 5.01e-19 79.0
MsG0480018311.01.T01 AT1G15790 58.974 78 32 0 18 95 4 81 3.36e-24 92.4
MsG0480018311.01.T01 AT1G15790 45.588 68 35 1 7 74 100 165 8.42e-12 60.1
MsG0480018311.01.T01 AT1G15790 58.974 78 32 0 18 95 4 81 3.71e-24 92.0
MsG0480018311.01.T01 AT1G15790 46.591 88 39 2 7 88 100 185 1.92e-16 72.4

Find 33 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAAACATCTTCCTGTTTC+TGG 0.115105 4:+3192893 MsG0480018311.01.T01:CDS
ATTTGATGGATTCCAATAAT+TGG 0.150937 4:+3192066 None:intergenic
CTGATTAGGAACCCAATTAT+TGG 0.216290 4:-3192078 None:intergenic
CAATAATTGGGTTCCTAATC+AGG 0.230649 4:+3192079 MsG0480018311.01.T01:CDS
AGGGAATTGGTGGTTGTATT+CGG 0.281870 4:+3193449 MsG0480018311.01.T01:CDS
CTGGGACCACCTTCATTTGA+TGG 0.310661 4:-3193389 None:intergenic
TTTGATGGATTCCAATAATT+GGG 0.376606 4:+3192067 None:intergenic
GGGAATTGGTGGTTGTATTC+GGG 0.380697 4:+3193450 MsG0480018311.01.T01:CDS
GTGGTTTCAGTACCCTGATT+AGG 0.384477 4:-3192092 None:intergenic
TAATTAAGATTTGCCAACTT+TGG 0.401624 4:-3193480 None:intergenic
TTCATTTGATGGCATATTGT+TGG 0.405356 4:-3193378 None:intergenic
ACAATTCTTTGGCGTTGATC+AGG 0.409125 4:-3192149 None:intergenic
CTTCCTGTTTCTGGTTCTGA+GGG 0.410128 4:+3192902 MsG0480018311.01.T01:CDS
CTGAGGGATTACTTGAACTT+CGG 0.419882 4:+3192918 MsG0480018311.01.T01:CDS
CCACTGCCGACACAGTTGAT+TGG 0.450196 4:+3192111 MsG0480018311.01.T01:CDS
TTGATCGGGCAGATTATTGC+TGG 0.458444 4:-3193408 None:intergenic
AATCCCTCAGAACCAGAAAC+AGG 0.458662 4:-3192905 None:intergenic
AAACATATGCTTACCTTGAT+CGG 0.461524 4:-3193423 None:intergenic
TCTTCCTGTTTCTGGTTCTG+AGG 0.467921 4:+3192901 MsG0480018311.01.T01:CDS
AGCAATAATCTGCCCGATCA+AGG 0.468625 4:+3193410 MsG0480018311.01.T01:CDS
CCAATCAACTGTGTCGGCAG+TGG 0.479669 4:-3192111 None:intergenic
AACAATATGCCATCAAATGA+AGG 0.481749 4:+3193380 MsG0480018311.01.T01:CDS
AACATATGCTTACCTTGATC+GGG 0.491877 4:-3193422 None:intergenic
TGATGGCATATTGTTGGCCA+TGG 0.498558 4:-3193372 None:intergenic
ATCTATGAAAATGCTAACAA+TGG 0.530496 4:+3193331 MsG0480018311.01.T01:CDS
TTCGGGTATGATACCAAAGT+TGG 0.533650 4:+3193467 MsG0480018311.01.T01:CDS
AATAATTGGGTTCCTAATCA+GGG 0.550108 4:+3192080 MsG0480018311.01.T01:CDS
TTATACTGCTGCGACAAGCC+AGG 0.552982 4:+3192970 MsG0480018311.01.T01:CDS
TGATCGGGCAGATTATTGCT+GGG 0.590486 4:-3193407 None:intergenic
AACTTCGGAAGATTGCTCAA+AGG 0.591413 4:+3192933 MsG0480018311.01.T01:CDS
AGTTCTCCAATCAACTGTGT+CGG 0.623037 4:-3192117 None:intergenic
AATATGCCATCAAATGAAGG+TGG 0.682135 4:+3193383 MsG0480018311.01.T01:CDS
GAATAAATCTCAAAACACCA+TGG 0.684380 4:+3193355 MsG0480018311.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCATTTCTTAATTTTCTT+TGG - Chr4:3192253-3192272 None:intergenic 15.0%
!! AAATGAATCAAATAAGTTAG+AGG + Chr4:3193145-3193164 MsG0480018311.01.T01:intron 20.0%
!! AATGAATCAAATAAGTTAGA+GGG + Chr4:3193146-3193165 MsG0480018311.01.T01:intron 20.0%
!! TTCAAATTAATACTCAACTA+TGG - Chr4:3192576-3192595 None:intergenic 20.0%
!! TTCAATTTCTGACAAATAAT+AGG + Chr4:3192442-3192461 MsG0480018311.01.T01:intron 20.0%
!!! ACAATTTGTTTCAAAATGAT+TGG - Chr4:3192635-3192654 None:intergenic 20.0%
! AATGAGAATAACTGTTTGTT+AGG - Chr4:3193119-3193138 None:intergenic 25.0%
! ACAAAAGAAATCTTCAACAA+AGG - Chr4:3192297-3192316 None:intergenic 25.0%
! ATCTATGAAAATGCTAACAA+TGG + Chr4:3193331-3193350 MsG0480018311.01.T01:CDS 25.0%
! GTTTCTACTATATTGAACAT+CGG + Chr4:3193253-3193272 MsG0480018311.01.T01:intron 25.0%
! TTCATCTGAAAAATAATGAG+AGG - Chr4:3192474-3192493 None:intergenic 25.0%
!! ACATTTTGTTGACAATTCTT+TGG - Chr4:3192163-3192182 None:intergenic 25.0%
!!! GAAAATATTTGGTAGTGATT+CGG - Chr4:3192507-3192526 None:intergenic 25.0%
!!! TCTCTATTTTTTTTGTTCTG+CGG + Chr4:3192726-3192745 MsG0480018311.01.T01:intron 25.0%
!!! TGTTAGGACTGTTATTTTAT+AGG - Chr4:3193103-3193122 None:intergenic 25.0%
!!! TTTTTTACTTCTTCCATTCT+TGG - Chr4:3192343-3192362 None:intergenic 25.0%
AAAAAAAGCAGTACGTTTAG+TGG + Chr4:3192363-3192382 MsG0480018311.01.T01:intron 30.0%
AAACATATGCTTACCTTGAT+CGG - Chr4:3193426-3193445 None:intergenic 30.0%
AACAATATGCCATCAAATGA+AGG + Chr4:3193380-3193399 MsG0480018311.01.T01:CDS 30.0%
AATAATTGGGTTCCTAATCA+GGG + Chr4:3192080-3192099 MsG0480018311.01.T01:CDS 30.0%
AGATAATCAGACTGAAAAGA+AGG - Chr4:3193302-3193321 None:intergenic 30.0%
CAACAAAGGTTGTCAATTTA+TGG - Chr4:3192283-3192302 None:intergenic 30.0%
GAATAAATCTCAAAACACCA+TGG + Chr4:3193355-3193374 MsG0480018311.01.T01:CDS 30.0%
! AAGGTAAGCATATGTTTTCT+AGG + Chr4:3193429-3193448 MsG0480018311.01.T01:CDS 30.0%
! AGGTAAGCATATGTTTTCTA+GGG + Chr4:3193430-3193449 MsG0480018311.01.T01:CDS 30.0%
! ATCACTACCAAATATTTTCG+AGG + Chr4:3192508-3192527 MsG0480018311.01.T01:intron 30.0%
! TTCATTTGATGGCATATTGT+TGG - Chr4:3193381-3193400 None:intergenic 30.0%
!! ATTGCTTTTCCTTTGTAGAA+TGG + Chr4:3192862-3192881 MsG0480018311.01.T01:intron 30.0%
!!! CTCTATTTTTTTTGTTCTGC+GGG + Chr4:3192727-3192746 MsG0480018311.01.T01:intron 30.0%
AACATATGCTTACCTTGATC+GGG - Chr4:3193425-3193444 None:intergenic 35.0%
AATATGCCATCAAATGAAGG+TGG + Chr4:3193383-3193402 MsG0480018311.01.T01:CDS 35.0%
AGAAAACATCTTCCTGTTTC+TGG + Chr4:3192893-3192912 MsG0480018311.01.T01:CDS 35.0%
AGCATGCTAAAAATCATGCA+TGG - Chr4:3192535-3192554 None:intergenic 35.0%
CAATAATTGGGTTCCTAATC+AGG + Chr4:3192079-3192098 MsG0480018311.01.T01:CDS 35.0%
CACAAACACAACAAGATTAG+CGG - Chr4:3192795-3192814 None:intergenic 35.0%
CAGTGTCAATATTGTATCAG+AGG + Chr4:3192189-3192208 MsG0480018311.01.T01:intron 35.0%
CATCGATGAATGATTATCCT+TGG + Chr4:3192671-3192690 MsG0480018311.01.T01:intron 35.0%
CTCTTTCTCAAAGATAGCAT+AGG + Chr4:3193231-3193250 MsG0480018311.01.T01:intron 35.0%
CTGATTAGGAACCCAATTAT+TGG - Chr4:3192081-3192100 None:intergenic 35.0%
GATGCAATTCTATCCAAGAA+TGG + Chr4:3192327-3192346 MsG0480018311.01.T01:intron 35.0%
TCGGTTATTTAACTCGATAC+TGG + Chr4:3193272-3193291 MsG0480018311.01.T01:intron 35.0%
TTAACGTGTCCATTCTACAA+AGG - Chr4:3192874-3192893 None:intergenic 35.0%
TTCAACTACTGTCTGTTTAG+AGG - Chr4:3192703-3192722 None:intergenic 35.0%
! TTTTAGCATGCTTAGGTTAG+CGG + Chr4:3192543-3192562 MsG0480018311.01.T01:intron 35.0%
!! GCATATGTTTTCTAGGGAAT+TGG + Chr4:3193436-3193455 MsG0480018311.01.T01:CDS 35.0%
!! TATGTTTTCTAGGGAATTGG+TGG + Chr4:3193439-3193458 MsG0480018311.01.T01:CDS 35.0%
!!! ATTTTTTTTGTTCTGCGGGT+GGG + Chr4:3192731-3192750 MsG0480018311.01.T01:intron 35.0%
!!! GCATGATTTTTAGCATGCTT+AGG + Chr4:3192536-3192555 MsG0480018311.01.T01:intron 35.0%
!!! TATTTTTTTTGTTCTGCGGG+TGG + Chr4:3192730-3192749 MsG0480018311.01.T01:intron 35.0%
AACTTCGGAAGATTGCTCAA+AGG + Chr4:3192933-3192952 MsG0480018311.01.T01:CDS 40.0%
AGTTCTCCAATCAACTGTGT+CGG - Chr4:3192120-3192139 None:intergenic 40.0%
CTGAGGGATTACTTGAACTT+CGG + Chr4:3192918-3192937 MsG0480018311.01.T01:CDS 40.0%
GCAGTCTCTACTTTCATTCA+TGG + Chr4:3193014-3193033 MsG0480018311.01.T01:intron 40.0%
GCATGGACCTCGAAAATATT+TGG - Chr4:3192518-3192537 None:intergenic 40.0%
GTACTCGTAATGAATGTACC+TGG - Chr4:3192991-3193010 None:intergenic 40.0%
TGACAGAGATGTTCAGACAA+TGG - Chr4:3192818-3192837 None:intergenic 40.0%
TTCGGGTATGATACCAAAGT+TGG + Chr4:3193467-3193486 MsG0480018311.01.T01:CDS 40.0%
! AGGGAATTGGTGGTTGTATT+CGG + Chr4:3193449-3193468 MsG0480018311.01.T01:CDS 40.0%
!! ACAATTCTTTGGCGTTGATC+AGG - Chr4:3192152-3192171 None:intergenic 40.0%
!!! TTTTTTTTGTTCTGCGGGTG+GGG + Chr4:3192732-3192751 MsG0480018311.01.T01:intron 40.0%
AATCCCTCAGAACCAGAAAC+AGG - Chr4:3192908-3192927 None:intergenic 45.0%
AGCAATAATCTGCCCGATCA+AGG + Chr4:3193410-3193429 MsG0480018311.01.T01:CDS 45.0%
AGGTTATACGCAGCTACAGT+AGG - Chr4:3193083-3193102 None:intergenic 45.0%
CTGTTTAGAGGACAGATCCA+AGG - Chr4:3192691-3192710 None:intergenic 45.0%
GTGGTTTCAGTACCCTGATT+AGG - Chr4:3192095-3192114 None:intergenic 45.0%
TGATCGGGCAGATTATTGCT+GGG - Chr4:3193410-3193429 None:intergenic 45.0%
TGATGGCATATTGTTGGCCA+TGG - Chr4:3193375-3193394 None:intergenic 45.0%
TTGATCGGGCAGATTATTGC+TGG - Chr4:3193411-3193430 None:intergenic 45.0%
! GGGAATTGGTGGTTGTATTC+GGG + Chr4:3193450-3193469 MsG0480018311.01.T01:CDS 45.0%
!! CTTCCTGTTTCTGGTTCTGA+GGG + Chr4:3192902-3192921 MsG0480018311.01.T01:CDS 45.0%
!! TCTTCCTGTTTCTGGTTCTG+AGG + Chr4:3192901-3192920 MsG0480018311.01.T01:CDS 45.0%
!!! TTTTTTTGTTCTGCGGGTGG+GGG + Chr4:3192733-3192752 MsG0480018311.01.T01:intron 45.0%
CTGGGACCACCTTCATTTGA+TGG - Chr4:3193392-3193411 None:intergenic 50.0%
TTATACTGCTGCGACAAGCC+AGG + Chr4:3192970-3192989 MsG0480018311.01.T01:CDS 50.0%
CCACTGCCGACACAGTTGAT+TGG + Chr4:3192111-3192130 MsG0480018311.01.T01:CDS 55.0%
! CCAATCAACTGTGTCGGCAG+TGG - Chr4:3192114-3192133 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 3192071 3193499 3192071 ID=MsG0480018311.01;Name=MsG0480018311.01
Chr4 mRNA 3192071 3193499 3192071 ID=MsG0480018311.01.T01;Parent=MsG0480018311.01;Name=MsG0480018311.01.T01;_AED=0.49;_eAED=0.51;_QI=0|1|0.66|1|1|1|3|0|136
Chr4 exon 3192071 3192180 3192071 ID=MsG0480018311.01.T01:exon:1332;Parent=MsG0480018311.01.T01
Chr4 exon 3192880 3192991 3192880 ID=MsG0480018311.01.T01:exon:1333;Parent=MsG0480018311.01.T01
Chr4 exon 3193311 3193499 3193311 ID=MsG0480018311.01.T01:exon:1334;Parent=MsG0480018311.01.T01
Chr4 CDS 3192071 3192180 3192071 ID=MsG0480018311.01.T01:cds;Parent=MsG0480018311.01.T01
Chr4 CDS 3192880 3192991 3192880 ID=MsG0480018311.01.T01:cds;Parent=MsG0480018311.01.T01
Chr4 CDS 3193311 3193499 3193311 ID=MsG0480018311.01.T01:cds;Parent=MsG0480018311.01.T01
Gene Sequence

>MsG0480018311.01.T01

ATGGATTCCAATAATTGGGTTCCTAATCAGGGTACTGAAACCACTGCCGACACAGTTGATTGGAGAACTCAACTGCAACCTGATCAACGCCAAAGAATTGTCAACAAAATAATGGACACGTTAAGAAAACATCTTCCTGTTTCTGGTTCTGAGGGATTACTTGAACTTCGGAAGATTGCTCAAAGGTTTGAAGATAAGATTTATACTGCTGCGACAAGCCAGTCTGATTATCTACGAAAAATATCTATGAAAATGCTAACAATGGAGAATAAATCTCAAAACACCATGGCCAACAATATGCCATCAAATGAAGGTGGTCCCAGCAATAATCTGCCCGATCAAGGTAAGCATATGTTTTCTAGGGAATTGGTGGTTGTATTCGGGTATGATACCAAAGTTGGCAAATCTTAA

Protein sequence

>MsG0480018311.01.T01

MDSNNWVPNQGTETTADTVDWRTQLQPDQRQRIVNKIMDTLRKHLPVSGSEGLLELRKIAQRFEDKIYTAATSQSDYLRKISMKMLTMENKSQNTMANNMPSNEGGPSNNLPDQGKHMFSRELVVVFGYDTKVGKS*