AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280011075.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011075.01.T01 MTR_2g097670 87.293 181 4 1 1 162 1 181 4.45e-104 318
MsG0280011075.01.T01 MTR_2g097670 87.293 181 4 1 1 162 1 181 1.35e-103 319
MsG0280011075.01.T01 MTR_4g064670 69.022 184 34 3 1 162 1 183 3.20e-64 211
MsG0280011075.01.T01 MTR_4g064670 69.022 184 34 3 1 162 1 183 4.23e-64 212
MsG0280011075.01.T01 MTR_5g080390 46.259 147 49 4 1 127 9 145 1.60e-31 119
MsG0280011075.01.T01 MTR_1g094630 39.394 165 78 4 18 161 18 181 4.85e-30 115
MsG0280011075.01.T01 MTR_1g094630 39.394 165 78 4 18 161 18 181 6.18e-30 114
MsG0280011075.01.T01 MTR_1g094630 39.394 165 78 4 18 161 49 212 7.40e-30 114
MsG0280011075.01.T01 MTR_2g036900 34.667 150 65 5 25 152 35 173 7.47e-13 65.9
MsG0280011075.01.T01 MTR_2g036900 34.667 150 65 5 25 152 35 173 7.93e-13 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011075.01.T01 AT2G29140 52.381 168 51 5 1 144 2 164 4.19e-46 160
MsG0280011075.01.T01 AT2G29140 52.381 168 51 5 1 144 2 164 4.19e-46 160
MsG0280011075.01.T01 AT2G29200 50.829 181 58 6 1 157 1 174 1.05e-45 159
MsG0280011075.01.T01 AT2G29200 50.829 181 58 6 1 157 1 174 1.05e-45 159
MsG0280011075.01.T01 AT2G29190 47.849 186 66 4 1 162 1 179 2.49e-45 159
MsG0280011075.01.T01 AT2G29190 47.849 186 66 4 1 162 1 179 2.49e-45 159
MsG0280011075.01.T01 AT3G10360 34.682 173 81 4 3 144 5 176 1.51e-22 93.6
MsG0280011075.01.T01 AT3G20250 44.737 76 23 1 50 106 59 134 9.85e-13 65.5
MsG0280011075.01.T01 AT3G20250 44.737 76 23 1 50 106 59 134 1.25e-12 65.1
MsG0280011075.01.T01 AT3G20250 44.737 76 23 1 50 106 59 134 1.25e-12 65.1

Find 52 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGAATTTGAAAAGGAAAT+AGG 0.195311 2:-79151479 MsG0280011075.01.T01:CDS
GGAGATAGGAGGAAGGTTAA+TGG 0.256678 2:-79151117 MsG0280011075.01.T01:CDS
GCTTCTGCAATTGGTGGAAT+TGG 0.342634 2:-79151138 MsG0280011075.01.T01:CDS
CTCAGAACCAAAACCATTCC+CGG 0.376480 2:+79151287 None:intergenic
ATGAATTTGAAAAGGAAATA+GGG 0.381115 2:-79151478 MsG0280011075.01.T01:CDS
GGTGGTAATGAGGGTTCGTT+TGG 0.397361 2:-79151503 MsG0280011075.01.T01:CDS
TAGCAAAGGAGGAGGAAGTC+TGG 0.420172 2:+79151203 None:intergenic
GCGGTGTTTGCTACACCTCC+GGG 0.431004 2:-79151072 MsG0280011075.01.T01:CDS
TGTTGTCTGATATGGGTAGA+AGG 0.432671 2:-79151538 MsG0280011075.01.T01:CDS
CTCCTATACAAATTAAGTTC+AGG 0.435558 2:+79151410 None:intergenic
AAAGGCGGAGCTTCTGCAAT+TGG 0.441477 2:-79151147 MsG0280011075.01.T01:CDS
AGCGGTGTTTGCTACACCTC+CGG 0.444772 2:-79151073 MsG0280011075.01.T01:CDS
CACCTCCGGGGTTTAACAAT+AGG 0.447693 2:-79151059 MsG0280011075.01.T01:CDS
CTTTGCTATCAAAGGAGGAT+TGG 0.447733 2:-79151188 MsG0280011075.01.T01:CDS
TGCTATCAAAGGAGGATTGG+AGG 0.460502 2:-79151185 MsG0280011075.01.T01:CDS
TTGGTTCTGAGGAAGAGCTT+AGG 0.467990 2:-79151275 MsG0280011075.01.T01:CDS
GTTTGGAGATGAATTTGAAA+AGG 0.470900 2:-79151486 MsG0280011075.01.T01:CDS
AACCTGAACTTAATTTGTAT+AGG 0.474825 2:-79151412 MsG0280011075.01.T01:CDS
TCCTCCTCCTTTGCTATCAA+AGG 0.479514 2:-79151196 MsG0280011075.01.T01:CDS
CAATTGGTGGAATTGGAGAT+AGG 0.481550 2:-79151131 MsG0280011075.01.T01:CDS
AGTGATTGGTGGTGGTAATG+AGG 0.481583 2:-79151513 MsG0280011075.01.T01:CDS
AACGAACCCTCCACAGTTGG+AGG 0.496406 2:+79151377 None:intergenic
AGCAAAGGAGGAGGAAGTCT+GGG 0.518907 2:+79151204 None:intergenic
GGCGGAGCTTCTGCAATTGG+TGG 0.520880 2:-79151144 MsG0280011075.01.T01:CDS
ATCAGCTCCTCCAACTGTGG+AGG 0.531581 2:-79151384 MsG0280011075.01.T01:CDS
TCATTACCACCACCAATCAC+TGG 0.534723 2:+79151515 None:intergenic
TCAGCTCCTCCAACTGTGGA+GGG 0.541998 2:-79151383 MsG0280011075.01.T01:intron
TGGATCAGCTCCTCCAACTG+TGG 0.546148 2:-79151387 MsG0280011075.01.T01:CDS
CTCAACGAACCCTCCACAGT+TGG 0.546176 2:+79151374 None:intergenic
GAACTTAATTTGTATAGGAG+TGG 0.547786 2:-79151407 MsG0280011075.01.T01:CDS
TCCTCCTTTGCTATCAAAGG+AGG 0.548140 2:-79151193 MsG0280011075.01.T01:CDS
GGATGTTGCTTCGCGATCAA+CGG 0.556585 2:-79151457 MsG0280011075.01.T01:CDS
GTGATTGGTGGTGGTAATGA+GGG 0.561511 2:-79151512 MsG0280011075.01.T01:CDS
GAAAAGAGAGACTGAGGCGG+TGG 0.566351 2:-79151037 MsG0280011075.01.T01:CDS
CAATCCTCCTTTGATAGCAA+AGG 0.571405 2:+79151189 None:intergenic
TCCTTTGATAGCAAAGGAGG+AGG 0.575676 2:+79151195 None:intergenic
GGTAGAAGGCCAGTGATTGG+TGG 0.580326 2:-79151524 MsG0280011075.01.T01:CDS
AGAAGGCCAGTGATTGGTGG+TGG 0.581232 2:-79151521 MsG0280011075.01.T01:CDS
TCGCGATCAACGGCGTCATG+AGG 0.581350 2:-79151447 MsG0280011075.01.T01:CDS
ATGGGTAGAAGGCCAGTGAT+TGG 0.582847 2:-79151527 MsG0280011075.01.T01:CDS
GTTAATGGAGCTGATGAGAA+TGG 0.589456 2:-79151102 MsG0280011075.01.T01:CDS
TGGAATTGGAGATAGGAGGA+AGG 0.594744 2:-79151124 MsG0280011075.01.T01:CDS
TCCTCCTTTGATAGCAAAGG+AGG 0.602969 2:+79151192 None:intergenic
TAATAATATTGAAGATACGC+AGG 0.625157 2:+79151246 None:intergenic
TTGGTGGAATTGGAGATAGG+AGG 0.626161 2:-79151128 MsG0280011075.01.T01:CDS
CAATAGGAAAAGAGAGACTG+AGG 0.630432 2:-79151043 MsG0280011075.01.T01:CDS
AATGGAGCTGATGAGAATGG+TGG 0.643341 2:-79151099 MsG0280011075.01.T01:CDS
CGGTGTTTGCTACACCTCCG+GGG 0.655610 2:-79151071 MsG0280011075.01.T01:CDS
TTCCTATTGTTAAACCCCGG+AGG 0.662875 2:+79151057 None:intergenic
GTGGCTGATGAGAAAACTAG+AGG 0.667175 2:-79151018 MsG0280011075.01.T01:CDS
TGATGAGAATGGTGGAAGAG+CGG 0.704265 2:-79151091 MsG0280011075.01.T01:CDS
TAGGAAAAGAGAGACTGAGG+CGG 0.753953 2:-79151040 MsG0280011075.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAATTTGAAAAGGAAATA+GGG - Chr2:79151069-79151088 MsG0280011075.01.T01:CDS 20.0%
! GATGAATTTGAAAAGGAAAT+AGG - Chr2:79151068-79151087 MsG0280011075.01.T01:CDS 25.0%
! TAATAATATTGAAGATACGC+AGG + Chr2:79151304-79151323 None:intergenic 25.0%
!!! AACCTGAACTTAATTTGTAT+AGG - Chr2:79151135-79151154 MsG0280011075.01.T01:CDS 25.0%
CTCCTATACAAATTAAGTTC+AGG + Chr2:79151140-79151159 None:intergenic 30.0%
GTTTGGAGATGAATTTGAAA+AGG - Chr2:79151061-79151080 MsG0280011075.01.T01:CDS 30.0%
!! GAACTTAATTTGTATAGGAG+TGG - Chr2:79151140-79151159 MsG0280011075.01.T01:CDS 30.0%
! CTTTTCCTATTGTTAAACCC+CGG + Chr2:79151496-79151515 None:intergenic 35.0%
CAATAGGAAAAGAGAGACTG+AGG - Chr2:79151504-79151523 MsG0280011075.01.T01:CDS 40.0%
CAATTGGTGGAATTGGAGAT+AGG - Chr2:79151416-79151435 MsG0280011075.01.T01:CDS 40.0%
GTTAATGGAGCTGATGAGAA+TGG - Chr2:79151445-79151464 MsG0280011075.01.T01:CDS 40.0%
TGTTGTCTGATATGGGTAGA+AGG - Chr2:79151009-79151028 MsG0280011075.01.T01:CDS 40.0%
! AAGTTTTCCGGGAATGGTTT+TGG - Chr2:79151253-79151272 MsG0280011075.01.T01:CDS 40.0%
! AGGTTTTCGCAGAGACTAAA+AGG - Chr2:79151382-79151401 MsG0280011075.01.T01:intron 40.0%
! CAATCCTCCTTTGATAGCAA+AGG + Chr2:79151361-79151380 None:intergenic 40.0%
! CTTTGCTATCAAAGGAGGAT+TGG - Chr2:79151359-79151378 MsG0280011075.01.T01:intron 40.0%
! TTTTCGCAGAGACTAAAAGG+CGG - Chr2:79151385-79151404 MsG0280011075.01.T01:CDS 40.0%
AATGGAGCTGATGAGAATGG+TGG - Chr2:79151448-79151467 MsG0280011075.01.T01:CDS 45.0%
CTCAGAACCAAAACCATTCC+CGG + Chr2:79151263-79151282 None:intergenic 45.0%
GCTTCTGCAATTGGTGGAAT+TGG - Chr2:79151409-79151428 MsG0280011075.01.T01:CDS 45.0%
GGAGATAGGAGGAAGGTTAA+TGG - Chr2:79151430-79151449 MsG0280011075.01.T01:CDS 45.0%
GTGGCTGATGAGAAAACTAG+AGG - Chr2:79151529-79151548 MsG0280011075.01.T01:CDS 45.0%
TAGGAAAAGAGAGACTGAGG+CGG - Chr2:79151507-79151526 MsG0280011075.01.T01:CDS 45.0%
TCATTACCACCACCAATCAC+TGG + Chr2:79151035-79151054 None:intergenic 45.0%
TGCTATCAAAGGAGGATTGG+AGG - Chr2:79151362-79151381 MsG0280011075.01.T01:intron 45.0%
TGGAATTGGAGATAGGAGGA+AGG - Chr2:79151423-79151442 MsG0280011075.01.T01:CDS 45.0%
TTCCTATTGTTAAACCCCGG+AGG + Chr2:79151493-79151512 None:intergenic 45.0%
TTGGTGGAATTGGAGATAGG+AGG - Chr2:79151419-79151438 MsG0280011075.01.T01:CDS 45.0%
! CTGCAGTTTCGAAGTTTTCC+GGG - Chr2:79151242-79151261 MsG0280011075.01.T01:CDS 45.0%
! GCTGCAGTTTCGAAGTTTTC+CGG - Chr2:79151241-79151260 MsG0280011075.01.T01:CDS 45.0%
! GTTTCGAAGTTTTCCGGGAA+TGG - Chr2:79151247-79151266 MsG0280011075.01.T01:CDS 45.0%
! TCCTCCTCCTTTGCTATCAA+AGG - Chr2:79151351-79151370 MsG0280011075.01.T01:intron 45.0%
! TCCTCCTTTGATAGCAAAGG+AGG + Chr2:79151358-79151377 None:intergenic 45.0%
! TCCTCCTTTGCTATCAAAGG+AGG - Chr2:79151354-79151373 MsG0280011075.01.T01:intron 45.0%
! TCCTTTGATAGCAAAGGAGG+AGG + Chr2:79151355-79151374 None:intergenic 45.0%
! TGATGAGAATGGTGGAAGAG+CGG - Chr2:79151456-79151475 MsG0280011075.01.T01:CDS 45.0%
!! AGTGATTGGTGGTGGTAATG+AGG - Chr2:79151034-79151053 MsG0280011075.01.T01:CDS 45.0%
!! GTGATTGGTGGTGGTAATGA+GGG - Chr2:79151035-79151054 MsG0280011075.01.T01:CDS 45.0%
!! TTGGTTCTGAGGAAGAGCTT+AGG - Chr2:79151272-79151291 MsG0280011075.01.T01:CDS 45.0%
AAAGGCGGAGCTTCTGCAAT+TGG - Chr2:79151400-79151419 MsG0280011075.01.T01:CDS 50.0%
AGCAAAGGAGGAGGAAGTCT+GGG + Chr2:79151346-79151365 None:intergenic 50.0%
ATGGGTAGAAGGCCAGTGAT+TGG - Chr2:79151020-79151039 MsG0280011075.01.T01:CDS 50.0%
CACCTCCGGGGTTTAACAAT+AGG - Chr2:79151488-79151507 MsG0280011075.01.T01:CDS 50.0%
GTTCGTTGAGTGCAGTTGGA+GGG - Chr2:79151186-79151205 MsG0280011075.01.T01:CDS 50.0%
TAGCAAAGGAGGAGGAAGTC+TGG + Chr2:79151347-79151366 None:intergenic 50.0%
! GGATGTTGCTTCGCGATCAA+CGG - Chr2:79151090-79151109 MsG0280011075.01.T01:CDS 50.0%
! GGTGGTAATGAGGGTTCGTT+TGG - Chr2:79151044-79151063 MsG0280011075.01.T01:CDS 50.0%
! GGTGTTTGGAGGTGGTTCTA+CGG - Chr2:79151207-79151226 MsG0280011075.01.T01:CDS 50.0%
!!! CGGGAATGGTTTTGGTTCTG+AGG - Chr2:79151261-79151280 MsG0280011075.01.T01:CDS 50.0%
AACGAACCCTCCACAGTTGG+AGG + Chr2:79151173-79151192 None:intergenic 55.0%
AGAAGGCCAGTGATTGGTGG+TGG - Chr2:79151026-79151045 MsG0280011075.01.T01:CDS 55.0%
ATCAGCTCCTCCAACTGTGG+AGG - Chr2:79151163-79151182 MsG0280011075.01.T01:CDS 55.0%
CTCAACGAACCCTCCACAGT+TGG + Chr2:79151176-79151195 None:intergenic 55.0%
GAAAAGAGAGACTGAGGCGG+TGG - Chr2:79151510-79151529 MsG0280011075.01.T01:CDS 55.0%
GAGGGTTCGTTGAGTGCAGT+TGG - Chr2:79151182-79151201 MsG0280011075.01.T01:CDS 55.0%
GGTAGAAGGCCAGTGATTGG+TGG - Chr2:79151023-79151042 MsG0280011075.01.T01:CDS 55.0%
GGTTCGTTGAGTGCAGTTGG+AGG - Chr2:79151185-79151204 MsG0280011075.01.T01:CDS 55.0%
TCAGCTCCTCCAACTGTGGA+GGG - Chr2:79151164-79151183 MsG0280011075.01.T01:CDS 55.0%
TGGATCAGCTCCTCCAACTG+TGG - Chr2:79151160-79151179 MsG0280011075.01.T01:CDS 55.0%
! AGCGGTGTTTGCTACACCTC+CGG - Chr2:79151474-79151493 MsG0280011075.01.T01:CDS 55.0%
!! AGTTGGAGGGTGTTTGGAGG+TGG - Chr2:79151199-79151218 MsG0280011075.01.T01:CDS 55.0%
!! GAGTGCAGTTGGAGGGTGTT+TGG - Chr2:79151193-79151212 MsG0280011075.01.T01:CDS 55.0%
!! TGCAGTTGGAGGGTGTTTGG+AGG - Chr2:79151196-79151215 MsG0280011075.01.T01:CDS 55.0%
GGCGGAGCTTCTGCAATTGG+TGG - Chr2:79151403-79151422 MsG0280011075.01.T01:CDS 60.0%
TCGCGATCAACGGCGTCATG+AGG - Chr2:79151100-79151119 MsG0280011075.01.T01:CDS 60.0%
! CGGTGTTTGCTACACCTCCG+GGG - Chr2:79151476-79151495 MsG0280011075.01.T01:CDS 60.0%
! GCGGTGTTTGCTACACCTCC+GGG - Chr2:79151475-79151494 MsG0280011075.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 79151008 79151561 79151008 ID=MsG0280011075.01;Name=MsG0280011075.01
Chr2 mRNA 79151008 79151561 79151008 ID=MsG0280011075.01.T01;Parent=MsG0280011075.01;Name=MsG0280011075.01.T01;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|1|1|2|0|163
Chr2 exon 79151384 79151561 79151384 ID=MsG0280011075.01.T01:exon:17484;Parent=MsG0280011075.01.T01
Chr2 exon 79151008 79151321 79151008 ID=MsG0280011075.01.T01:exon:17483;Parent=MsG0280011075.01.T01
Chr2 CDS 79151384 79151561 79151384 ID=MsG0280011075.01.T01:cds;Parent=MsG0280011075.01.T01
Chr2 CDS 79151008 79151321 79151008 ID=MsG0280011075.01.T01:cds;Parent=MsG0280011075.01.T01
Gene Sequence

>MsG0280011075.01.T01

ATGTTGTCTGATATGGGTAGAAGGCCAGTGATTGGTGGTGGTAATGAGGGTTCGTTTGGAGATGAATTTGAAAAGGAAATAGGGATGTTGCTTCGCGATCAACGGCGTCATGAGGTTGATGATCATGAACCTGAACTTAATTTGTATAGGAGTGGATCAGCTCCTCCAACTGTGGAGGTTTCGAAGTTTTCCGGGAATGGTTTTGGTTCTGAGGAAGAGCTTAGGTCTGATCCTGCGTATCTTCAATATTATTACTCTAATGTTAATTTGAATCCCAGACTTCCTCCTCCTTTGCTATCAAAGGAGGATTGGAGGTTTTCGCAGAGACTAAAAGGCGGAGCTTCTGCAATTGGTGGAATTGGAGATAGGAGGAAGGTTAATGGAGCTGATGAGAATGGTGGAAGAGCGGTGTTTGCTACACCTCCGGGGTTTAACAATAGGAAAAGAGAGACTGAGGCGGTGGCTGATGAGAAAACTAGAGGTTCTGACTGA

Protein sequence

>MsG0280011075.01.T01

MLSDMGRRPVIGGGNEGSFGDEFEKEIGMLLRDQRRHEVDDHEPELNLYRSGSAPPTVEVSKFSGNGFGSEEELRSDPAYLQYYYSNVNLNPRLPPPLLSKEDWRFSQRLKGGASAIGGIGDRRKVNGADENGGRAVFATPPGFNNRKRETEAVADEKTRGSD*