Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024380.01.T01 | XP_003611048.1 | 69.231 | 169 | 17 | 4 | 44 | 177 | 159 | 327 | 1.65E-62 | 209 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024380.01.T01 | G7KBZ0 | 69.231 | 169 | 17 | 4 | 44 | 177 | 159 | 327 | 7.86e-63 | 209 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080049140.01 | MsG0580024380.01 | 0.800746 | 1.247234e-48 | 5.852908e-46 |
| MsG0180002583.01 | MsG0580024380.01 | 0.815211 | 1.038527e-51 | 7.115135e-49 |
| MsG0180003427.01 | MsG0580024380.01 | 0.800009 | 1.761678e-48 | 8.113973e-46 |
| MsG0280006311.01 | MsG0580024380.01 | 0.809824 | 1.563553e-50 | 9.274496e-48 |
| MsG0280011154.01 | MsG0580024380.01 | 0.830006 | 3.743982e-55 | 3.895596e-52 |
| MsG0380012788.01 | MsG0580024380.01 | 0.812454 | 4.206541e-51 | 2.675884e-48 |
| MsG0380012808.01 | MsG0580024380.01 | 0.823655 | 1.232412e-53 | 1.067786e-50 |
| MsG0480020463.01 | MsG0580024380.01 | 0.807027 | 6.176522e-50 | 3.403680e-47 |
| MsG0480020847.01 | MsG0580024380.01 | 0.805789 | 1.126518e-49 | 6.014060e-47 |
| MsG0480022982.01 | MsG0580024380.01 | 0.809410 | 1.918291e-50 | 1.125510e-47 |
| MsG0480022998.01 | MsG0580024380.01 | 0.821150 | 4.705604e-53 | 3.797798e-50 |
| MsG0480023296.01 | MsG0580024380.01 | 0.806579 | 7.680114e-50 | 4.183569e-47 |
| MsG0480023458.01 | MsG0580024380.01 | 0.801551 | 8.536894e-49 | 4.088447e-46 |
| MsG0580024380.01 | MsG0580028756.01 | 0.828431 | 9.027520e-55 | 8.971480e-52 |
| MsG0580024380.01 | MsG0780035954.01 | 0.814112 | 1.818671e-51 | 1.209551e-48 |
| MsG0580024380.01 | MsG0780039861.01 | 0.815265 | 1.010208e-51 | 6.931754e-49 |
| MsG0580024380.01 | MsG0780040620.01 | 0.818419 | 1.979939e-52 | 1.481388e-49 |
| MsG0580024380.01 | MsG0780041063.01 | 0.811873 | 5.630717e-51 | 3.525677e-48 |
| MsG0580024380.01 | MsG0780041132.01 | 0.809008 | 2.339421e-50 | 1.357812e-47 |
| MsG0580024380.01 | MsG0780041340.01 | 0.803278 | 3.761719e-49 | 1.882580e-46 |
| MsG0580024380.01 | MsG0780041579.01 | 0.803329 | 3.672101e-49 | 1.840096e-46 |
| MsG0580024380.01 | MsG0880043637.01 | 0.800398 | 1.468559e-48 | 6.831103e-46 |
| MsG0580024380.01 | MsG0880047059.01 | 0.835575 | 1.549020e-56 | 1.901937e-53 |
| MsG0380017164.01 | MsG0580024380.01 | 0.832499 | 9.128431e-56 | 1.022177e-52 |
| MsG0480018397.01 | MsG0580024380.01 | 0.826706 | 2.341936e-54 | 2.213121e-51 |
| MsG0280006540.01 | MsG0580024380.01 | 0.827293 | 1.695119e-54 | 1.629623e-51 |
| MsG0280006563.01 | MsG0580024380.01 | 0.810411 | 1.168149e-50 | 7.036224e-48 |
| MsG0280009752.01 | MsG0580024380.01 | 0.800203 | 1.608835e-48 | 7.446506e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024380.01.T01 | MTR_5g009870 | 69.231 | 169 | 17 | 4 | 44 | 177 | 159 | 327 | 1.99e-66 | 209 |
| MsG0580024380.01.T01 | MTR_8g099045 | 36.098 | 205 | 62 | 7 | 37 | 175 | 95 | 296 | 2.00e-16 | 76.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 36 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGTTGTTTACAAACATTT+AGG | 0.218894 | 5:-4056024 | MsG0580024380.01.T01:CDS |
| AAGTTTCGTGCTTTCATGTC+TGG | 0.339977 | 5:-4056454 | MsG0580024380.01.T01:CDS |
| CAAGAGTCAGAGTCAGAAAC+AGG | 0.350794 | 5:-4056070 | MsG0580024380.01.T01:CDS |
| GTGAATTGAGGTTTGTTGTT+TGG | 0.372570 | 5:+4056502 | None:intergenic |
| GAGTTGTTTACAAACATTTA+GGG | 0.374177 | 5:-4056023 | MsG0580024380.01.T01:CDS |
| GAAGAGCCGTTCCGCGTCTT+CGG | 0.426597 | 5:-4056001 | MsG0580024380.01.T01:CDS |
| CTGTTTCTGACTCTGACTCT+TGG | 0.446943 | 5:+4056071 | None:intergenic |
| AACAATGAAGCAGTGAATTG+AGG | 0.470671 | 5:+4056490 | None:intergenic |
| TAAACAACTCTTAGCCCTGC+TGG | 0.473959 | 5:+4056036 | None:intergenic |
| CCGTTCCGCGTCTTCGGCAG+TGG | 0.475358 | 5:-4055995 | MsG0580024380.01.T01:CDS |
| GGCAGTGGCGGCTTCTCCGC+CGG | 0.476606 | 5:-4055980 | MsG0580024380.01.T01:CDS |
| TCAGAGGATTTATTCTTCAA+AGG | 0.479259 | 5:-4056588 | MsG0580024380.01.T01:CDS |
| TCTTTCACTGTCACCTTCAG+AGG | 0.491323 | 5:-4056604 | MsG0580024380.01.T01:CDS |
| CAGGGGAATAATATTCCAGC+AGG | 0.508754 | 5:-4056051 | MsG0580024380.01.T01:CDS |
| CCATGGTGATAAATCAAGCC+CGG | 0.510297 | 5:-4056157 | MsG0580024380.01.T01:CDS |
| TGAAAAGAAGCAAAACAAAC+CGG | 0.511879 | 5:-4056401 | MsG0580024380.01.T01:CDS |
| AGACATGAAAGCACGAAACT+TGG | 0.512267 | 5:+4056456 | None:intergenic |
| GTTGTTGTTGTTGAGAATGA+AGG | 0.513764 | 5:+4056541 | None:intergenic |
| ATTTCTGCTTCAATTTCACC+CGG | 0.514188 | 5:+4056138 | None:intergenic |
| CTGGTGAAAAGTGAAACAAT+AGG | 0.539536 | 5:+4056349 | None:intergenic |
| AGCCGCCACTGCCGAAGACG+CGG | 0.542768 | 5:+4055990 | None:intergenic |
| TTCCGCGTCTTCGGCAGTGG+CGG | 0.563880 | 5:-4055992 | MsG0580024380.01.T01:CDS |
| CCACTGCCGAAGACGCGGAA+CGG | 0.563993 | 5:+4055995 | None:intergenic |
| TTGAAGAATAAATCCTCTGA+AGG | 0.584940 | 5:+4056591 | None:intergenic |
| CTTTAATAAGGGTTTCTCGT+AGG | 0.589961 | 5:-4056185 | MsG0580024380.01.T01:intron |
| AAGAGTCAGAGTCAGAAACA+GGG | 0.601317 | 5:-4056069 | MsG0580024380.01.T01:CDS |
| CATGGTGATAAATCAAGCCC+GGG | 0.603985 | 5:-4056156 | MsG0580024380.01.T01:CDS |
| AGAGTCAGAGTCAGAAACAG+GGG | 0.634295 | 5:-4056068 | MsG0580024380.01.T01:CDS |
| GAAGCAGAAATAGCGACGGA+AGG | 0.649857 | 5:-4056126 | MsG0580024380.01.T01:CDS |
| TTTCTGCTTCAATTTCACCC+GGG | 0.658049 | 5:+4056139 | None:intergenic |
| GCGTTTCGACGACACTACAC+CGG | 0.662105 | 5:+4055961 | None:intergenic |
| CCGGGCTTGATTTATCACCA+TGG | 0.662133 | 5:+4056157 | None:intergenic |
| GTTTCTCGTAGGTACAGCCA+TGG | 0.666123 | 5:-4056174 | MsG0580024380.01.T01:CDS |
| AATTGAAGCAGAAATAGCGA+CGG | 0.696696 | 5:-4056130 | MsG0580024380.01.T01:CDS |
| TTTCGACGACACTACACCGG+CGG | 0.716664 | 5:+4055964 | None:intergenic |
| AGGGGAATAATATTCCAGCA+GGG | 0.717052 | 5:-4056050 | MsG0580024380.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGAGTTGTTTACAAACATTT+AGG | - | Chr5:4056573-4056592 | MsG0580024380.01.T01:CDS | 25.0% |
| ! | GAGTTGTTTACAAACATTTA+GGG | - | Chr5:4056574-4056593 | MsG0580024380.01.T01:CDS | 25.0% |
| ! | TATGCACAAGTATTTGAAAA+TGG | - | Chr5:4056381-4056400 | MsG0580024380.01.T01:CDS | 25.0% |
| !! | ACTTTGAATTTTATTCTCTC+CGG | + | Chr5:4056218-4056237 | None:intergenic | 25.0% |
| ATGGTGAAACCTCTTTAATA+AGG | - | Chr5:4056400-4056419 | MsG0580024380.01.T01:CDS | 30.0% | |
| TCAGAGGATTTATTCTTCAA+AGG | - | Chr5:4056009-4056028 | MsG0580024380.01.T01:CDS | 30.0% | |
| TGAAAAGAAGCAAAACAAAC+CGG | - | Chr5:4056196-4056215 | MsG0580024380.01.T01:intron | 30.0% | |
| TGGTGAAACCTCTTTAATAA+GGG | - | Chr5:4056401-4056420 | MsG0580024380.01.T01:CDS | 30.0% | |
| TTGAAGAATAAATCCTCTGA+AGG | + | Chr5:4056009-4056028 | None:intergenic | 30.0% | |
| ! | AGAAAACGTTTTTCTTCCAA+AGG | + | Chr5:4056348-4056367 | None:intergenic | 30.0% |
| ! | ATGGTTCATGTTCAACTTTT+TGG | + | Chr5:4056318-4056337 | None:intergenic | 30.0% |
| !! | CTTCTTTTCAAGAACAGAAT+CGG | + | Chr5:4056186-4056205 | None:intergenic | 30.0% |
| !!! | TTGAGATTTGTTGCTGTTTT+TGG | + | Chr5:4056284-4056303 | None:intergenic | 30.0% |
| AACAATGAAGCAGTGAATTG+AGG | + | Chr5:4056110-4056129 | None:intergenic | 35.0% | |
| AATTGAAGCAGAAATAGCGA+CGG | - | Chr5:4056467-4056486 | MsG0580024380.01.T01:CDS | 35.0% | |
| ACGAGAAACCCTTATTAAAG+AGG | + | Chr5:4056412-4056431 | None:intergenic | 35.0% | |
| ATGAACCATCTTATCACCTT+TGG | - | Chr5:4056329-4056348 | MsG0580024380.01.T01:CDS | 35.0% | |
| ATTTCTGCTTCAATTTCACC+CGG | + | Chr5:4056462-4056481 | None:intergenic | 35.0% | |
| CTGGTGAAAAGTGAAACAAT+AGG | + | Chr5:4056251-4056270 | None:intergenic | 35.0% | |
| CTTTAATAAGGGTTTCTCGT+AGG | - | Chr5:4056412-4056431 | MsG0580024380.01.T01:CDS | 35.0% | |
| TTCTTCCAAAGGTGATAAGA+TGG | + | Chr5:4056337-4056356 | None:intergenic | 35.0% | |
| ! | GAAGAAAAACGTTTTCTCCA+AGG | - | Chr5:4056351-4056370 | MsG0580024380.01.T01:CDS | 35.0% |
| ! | GTTGTTGTTGTTGAGAATGA+AGG | + | Chr5:4056059-4056078 | None:intergenic | 35.0% |
| ! | GTTTTCTTCACTTTCAGCTA+CGG | + | Chr5:4056502-4056521 | None:intergenic | 35.0% |
| !! | GTGAATTGAGGTTTGTTGTT+TGG | + | Chr5:4056098-4056117 | None:intergenic | 35.0% |
| !! | TCAAGAACAGAATCGGTTTT+TGG | + | Chr5:4056179-4056198 | None:intergenic | 35.0% |
| !!! | CATGTTCAACTTTTTGGTTC+TGG | + | Chr5:4056312-4056331 | None:intergenic | 35.0% |
| AAGAGTCAGAGTCAGAAACA+GGG | - | Chr5:4056528-4056547 | MsG0580024380.01.T01:CDS | 40.0% | |
| AAGTTTCGTGCTTTCATGTC+TGG | - | Chr5:4056143-4056162 | MsG0580024380.01.T01:CDS | 40.0% | |
| AGGGGAATAATATTCCAGCA+GGG | - | Chr5:4056547-4056566 | MsG0580024380.01.T01:CDS | 40.0% | |
| CTTGTGCATAACTACTTCCT+TGG | + | Chr5:4056371-4056390 | None:intergenic | 40.0% | |
| TTTCTGCTTCAATTTCACCC+GGG | + | Chr5:4056461-4056480 | None:intergenic | 40.0% | |
| ! | AGACATGAAAGCACGAAACT+TGG | + | Chr5:4056144-4056163 | None:intergenic | 40.0% |
| !!! | TGTTTTTGGAACTGTGTCTC+TGG | + | Chr5:4056270-4056289 | None:intergenic | 40.0% |
| AGAGTCAGAGTCAGAAACAG+GGG | - | Chr5:4056529-4056548 | MsG0580024380.01.T01:CDS | 45.0% | |
| CAAGAGTCAGAGTCAGAAAC+AGG | - | Chr5:4056527-4056546 | MsG0580024380.01.T01:CDS | 45.0% | |
| CAGGGGAATAATATTCCAGC+AGG | - | Chr5:4056546-4056565 | MsG0580024380.01.T01:CDS | 45.0% | |
| CATGGTGATAAATCAAGCCC+GGG | - | Chr5:4056441-4056460 | MsG0580024380.01.T01:CDS | 45.0% | |
| CCATGGTGATAAATCAAGCC+CGG | - | Chr5:4056440-4056459 | MsG0580024380.01.T01:CDS | 45.0% | |
| TAAACAACTCTTAGCCCTGC+TGG | + | Chr5:4056564-4056583 | None:intergenic | 45.0% | |
| TCTTTCACTGTCACCTTCAG+AGG | - | Chr5:4055993-4056012 | MsG0580024380.01.T01:CDS | 45.0% | |
| !! | CTGTTTCTGACTCTGACTCT+TGG | + | Chr5:4056529-4056548 | None:intergenic | 45.0% |
| CCGGGCTTGATTTATCACCA+TGG | + | Chr5:4056443-4056462 | None:intergenic | 50.0% | |
| GAAGCAGAAATAGCGACGGA+AGG | - | Chr5:4056471-4056490 | MsG0580024380.01.T01:CDS | 50.0% | |
| GTTTCTCGTAGGTACAGCCA+TGG | - | Chr5:4056423-4056442 | MsG0580024380.01.T01:CDS | 50.0% | |
| GAAGAGCCGTTCCGCGTCTT+CGG | - | Chr5:4056596-4056615 | MsG0580024380.01.T01:CDS | 60.0% | |
| CCACTGCCGAAGACGCGGAA+CGG | + | Chr5:4056605-4056624 | None:intergenic | 65.0% | |
| TTCCGCGTCTTCGGCAGTGG+CGG | - | Chr5:4056605-4056624 | MsG0580024380.01.T01:CDS | 65.0% | |
| AGCCGCCACTGCCGAAGACG+CGG | + | Chr5:4056610-4056629 | None:intergenic | 70.0% | |
| CCGTTCCGCGTCTTCGGCAG+TGG | - | Chr5:4056602-4056621 | MsG0580024380.01.T01:CDS | 70.0% | |
| GGCAGTGGCGGCTTCTCCGC+CGG | - | Chr5:4056617-4056636 | MsG0580024380.01.T01:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 4055975 | 4056644 | 4055975 | ID=MsG0580024380.01;Name=MsG0580024380.01 |
| Chr5 | mRNA | 4055975 | 4056644 | 4055975 | ID=MsG0580024380.01.T01;Parent=MsG0580024380.01;Name=MsG0580024380.01.T01;_AED=0.33;_eAED=0.42;_QI=0|0|0|1|1|1|2|0|189 |
| Chr5 | exon | 4056298 | 4056644 | 4056298 | ID=MsG0580024380.01.T01:exon:2200;Parent=MsG0580024380.01.T01 |
| Chr5 | exon | 4055975 | 4056197 | 4055975 | ID=MsG0580024380.01.T01:exon:2199;Parent=MsG0580024380.01.T01 |
| Chr5 | CDS | 4056298 | 4056644 | 4056298 | ID=MsG0580024380.01.T01:cds;Parent=MsG0580024380.01.T01 |
| Chr5 | CDS | 4055975 | 4056197 | 4055975 | ID=MsG0580024380.01.T01:cds;Parent=MsG0580024380.01.T01 |
| Gene Sequence |
| Protein sequence |
