AlfalfaGEDB | MsG0580024748.01

M. sativa cultivar ZhongmuNo.1 / MsG0580024748.01

KEGG: -
GO: -
PFAM: PF07983

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024748.01.T02	AFK38186.1	95.055	182	9	0	1	182	183	364	4.73E-109	330
MsG0580024748.01.T01	XP_003611601.1	94.702	151	6	1	1	151	1	149	8.79E-94	287

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024748.01.T01	G7K3T9	94.702	151	6	1	1	151	1	149	4.20e-94	287
MsG0580024748.01.T02	I3SD44	95.055	182	9	0	1	182	183	364	2.26e-109	330

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047815.01	MsG0580024748.01	0.805102	1.569890e-49	8.234113e-47
MsG0080048258.01	MsG0580024748.01	0.808525	2.968010e-50	1.700927e-47
MsG0080048775.01	MsG0580024748.01	0.825336	4.954754e-54	4.503245e-51
MsG0080048781.01	MsG0580024748.01	0.819082	1.400062e-52	1.066790e-49
MsG0080049140.01	MsG0580024748.01	0.821402	4.116053e-53	3.345252e-50
MsG0180000111.01	MsG0580024748.01	0.815421	9.325228e-52	6.426295e-49
MsG0180000314.01	MsG0580024748.01	0.807482	4.946197e-50	2.758178e-47
MsG0180002218.01	MsG0580024748.01	0.807816	4.201977e-50	2.363542e-47
MsG0180002397.01	MsG0580024748.01	0.841923	3.538458e-58	5.278562e-55
MsG0180002583.01	MsG0580024748.01	0.827669	1.377163e-54	1.338419e-51
MsG0180003360.01	MsG0580024748.01	0.800725	1.259264e-48	5.906320e-46
MsG0180003427.01	MsG0580024748.01	0.831399	1.706996e-55	1.850171e-52
MsG0180003485.01	MsG0580024748.01	0.854865	9.258996e-62	2.097397e-58
MsG0180003658.01	MsG0580024748.01	0.809023	2.322762e-50	1.348656e-47
MsG0180003698.01	MsG0580024748.01	0.847556	1.073041e-59	1.914583e-56
MsG0180003796.01	MsG0580024748.01	0.846248	2.447793e-59	4.186989e-56
MsG0180003857.01	MsG0580024748.01	0.806178	9.331652e-50	5.031697e-47
MsG0180004098.01	MsG0580024748.01	0.800714	1.265933e-48	5.935741e-46
MsG0180004299.01	MsG0580024748.01	0.807803	4.229399e-50	2.378172e-47
MsG0180004538.01	MsG0580024748.01	0.848720	5.121219e-60	9.485233e-57
MsG0180004583.01	MsG0580024748.01	0.841002	6.188071e-58	8.969060e-55
MsG0180004995.01	MsG0580024748.01	0.840556	8.098907e-58	1.157868e-54
MsG0180005048.01	MsG0580024748.01	0.819128	1.366758e-52	1.042715e-49
MsG0180005435.01	MsG0580024748.01	0.805526	1.279134e-49	6.782510e-47
MsG0180005835.01	MsG0580024748.01	0.802518	5.400563e-49	2.650753e-46
MsG0180006133.01	MsG0580024748.01	0.845640	3.579450e-59	6.004384e-56
MsG0280006311.01	MsG0580024748.01	0.802098	6.590996e-49	3.200730e-46
MsG0280010579.01	MsG0580024748.01	0.832354	9.916504e-56	1.105619e-52
MsG0280011083.01	MsG0580024748.01	0.813648	2.300797e-51	1.511138e-48
MsG0380012788.01	MsG0580024748.01	0.823207	1.568432e-53	1.341674e-50
MsG0380012808.01	MsG0580024748.01	0.808974	2.379619e-50	1.379890e-47
MsG0380013636.01	MsG0580024748.01	0.841398	4.867021e-58	7.143102e-55
MsG0380014594.01	MsG0580024748.01	0.823762	1.163132e-53	1.010834e-50
MsG0380014666.01	MsG0580024748.01	0.818129	2.303090e-52	1.709300e-49
MsG0480018526.01	MsG0580024748.01	0.848217	7.054208e-60	1.285217e-56
MsG0480018785.01	MsG0580024748.01	0.842256	2.890341e-58	4.357377e-55
MsG0480020366.01	MsG0580024748.01	0.807164	5.776703e-50	3.194708e-47
MsG0480020463.01	MsG0580024748.01	0.810358	1.199214e-50	7.213470e-48
MsG0480020856.01	MsG0580024748.01	0.800549	1.367786e-48	6.386726e-46
MsG0480021449.01	MsG0580024748.01	0.814330	1.627601e-51	1.088938e-48
MsG0480021454.01	MsG0580024748.01	0.824119	9.593650e-54	8.420452e-51
MsG0480021456.01	MsG0580024748.01	0.804171	2.455057e-49	1.257179e-46
MsG0480021901.01	MsG0580024748.01	0.834780	2.458813e-56	2.947604e-53
MsG0480021902.01	MsG0580024748.01	0.823094	1.666801e-53	1.421345e-50
MsG0480022331.01	MsG0580024748.01	0.812299	4.547462e-51	2.880580e-48
MsG0480022559.01	MsG0580024748.01	0.846018	2.825865e-59	4.798709e-56
MsG0480022784.01	MsG0580024748.01	0.852981	3.230982e-61	6.874062e-58
MsG0480022931.01	MsG0580024748.01	0.823006	1.747144e-53	1.486195e-50
MsG0480022982.01	MsG0580024748.01	0.822134	2.786810e-53	2.312390e-50
MsG0480022991.01	MsG0580024748.01	0.817455	3.269308e-52	2.382074e-49
MsG0480022998.01	MsG0580024748.01	0.843324	1.502544e-58	2.342790e-55
MsG0480023003.01	MsG0580024748.01	0.839722	1.336896e-57	1.862297e-54
MsG0480023133.01	MsG0580024748.01	0.819605	1.064096e-52	8.226388e-50
MsG0480023194.01	MsG0580024748.01	0.838377	2.982335e-57	3.987047e-54
MsG0480023262.01	MsG0580024748.01	0.805148	1.535608e-49	8.063878e-47
MsG0480023275.01	MsG0580024748.01	0.809515	1.821399e-50	1.071586e-47
MsG0480023296.01	MsG0580024748.01	0.837930	3.886488e-57	5.125555e-54
MsG0480023458.01	MsG0580024748.01	0.832908	7.225739e-56	8.190260e-53
MsG0480023653.01	MsG0580024748.01	0.804425	2.174202e-49	1.120593e-46
MsG0480023672.01	MsG0580024748.01	0.852123	5.674620e-61	1.173935e-57
MsG0580024742.01	MsG0580024748.01	0.819447	1.155729e-52	8.896131e-50
MsG0580024748.01	MsG0580024787.01	0.842660	2.257560e-58	3.446494e-55
MsG0580024748.01	MsG0580024813.01	0.821904	3.149839e-53	2.596636e-50
MsG0580024748.01	MsG0580024954.01	0.819432	1.164776e-52	8.961909e-50
MsG0580024748.01	MsG0580025177.01	0.819856	9.321781e-53	7.257755e-50
MsG0580024748.01	MsG0580025186.01	0.821309	4.324296e-53	3.505458e-50
MsG0580024748.01	MsG0580025203.01	0.831698	1.440095e-55	1.574992e-52
MsG0580024748.01	MsG0580025504.01	0.804987	1.658841e-49	8.675161e-47
MsG0580024748.01	MsG0580025506.01	0.851602	7.977407e-61	1.623104e-57
MsG0580024748.01	MsG0580025775.01	0.839248	1.774984e-57	2.436882e-54
MsG0580024748.01	MsG0580028756.01	0.848753	5.014899e-60	9.297914e-57
MsG0580024748.01	MsG0580028923.01	0.809058	2.282568e-50	1.326616e-47
MsG0580024748.01	MsG0580029102.01	0.818539	1.859740e-52	1.395984e-49
MsG0580024748.01	MsG0580029123.01	0.838141	3.429086e-57	4.551100e-54
MsG0580024748.01	MsG0580029261.01	0.821308	4.326106e-53	3.506838e-50
MsG0580024748.01	MsG0580029298.01	0.833768	4.410386e-56	5.129809e-53
MsG0580024748.01	MsG0580029591.01	0.850981	1.194859e-60	2.382714e-57
MsG0580024748.01	MsG0680031701.01	0.826110	3.246677e-54	3.016481e-51
MsG0580024748.01	MsG0680032773.01	0.812543	4.020928e-51	2.563801e-48
MsG0580024748.01	MsG0680035350.01	0.806520	7.903525e-50	4.298826e-47
MsG0580024748.01	MsG0680035878.01	0.800749	1.245620e-48	5.845720e-46
MsG0580024748.01	MsG0780035954.01	0.814377	1.589454e-51	1.064762e-48
MsG0580024748.01	MsG0780036534.01	0.822597	2.175776e-53	1.829597e-50
MsG0580024748.01	MsG0780037741.01	0.810832	9.474363e-51	5.771538e-48
MsG0580024748.01	MsG0780038402.01	0.846468	2.130913e-59	3.671752e-56
MsG0580024748.01	MsG0780038736.01	0.831363	1.742143e-55	1.886306e-52
MsG0580024748.01	MsG0780039861.01	0.863613	2.196608e-64	6.699932e-61
MsG0580024748.01	MsG0780039878.01	0.833868	4.163570e-56	4.857542e-53
MsG0580024748.01	MsG0780040474.01	0.804741	1.867815e-49	9.706725e-47
MsG0580024748.01	MsG0780040533.01	0.834023	3.807875e-56	4.462918e-53
MsG0580024748.01	MsG0780040620.01	0.864278	1.364219e-64	4.259459e-61
MsG0580024748.01	MsG0780040641.01	0.823975	1.037081e-53	9.066183e-51
MsG0580024748.01	MsG0780041008.01	0.818245	2.168023e-52	1.614114e-49
MsG0580024748.01	MsG0780041063.01	0.822889	1.860016e-53	1.576950e-50
MsG0580024748.01	MsG0780041132.01	0.852731	3.807324e-61	8.031463e-58
MsG0580024748.01	MsG0780041143.01	0.824751	6.813266e-54	6.090206e-51
MsG0580024748.01	MsG0780041257.01	0.816467	5.447135e-52	3.862692e-49
MsG0580024748.01	MsG0780041340.01	0.827974	1.163150e-54	1.140652e-51
MsG0580024748.01	MsG0780041359.01	0.857199	1.920501e-62	4.702006e-59
MsG0580024748.01	MsG0880041930.01	0.823193	1.580345e-53	1.351364e-50
MsG0580024748.01	MsG0880042773.01	0.816211	6.217445e-52	4.378424e-49
MsG0580024748.01	MsG0880043637.01	0.824340	8.509448e-54	7.515732e-51
MsG0580024748.01	MsG0880045430.01	0.804153	2.476612e-49	1.267640e-46
MsG0580024748.01	MsG0880045612.01	0.844463	7.445153e-59	1.202843e-55
MsG0580024748.01	MsG0880045775.01	0.825030	5.852652e-54	5.273509e-51
MsG0580024748.01	MsG0880045890.01	0.834766	2.478685e-56	2.970217e-53
MsG0580024748.01	MsG0880046208.01	0.841119	5.764791e-58	8.385894e-55
MsG0580024748.01	MsG0880046319.01	0.842205	2.980272e-58	4.485475e-55
MsG0580024748.01	MsG0880046431.01	0.810353	1.202355e-50	7.231281e-48
MsG0580024748.01	MsG0880046589.01	0.800409	1.460792e-48	6.797069e-46
MsG0580024748.01	MsG0880046646.01	0.827933	1.189816e-54	1.165330e-51
MsG0580024748.01	MsG0880046672.01	0.807205	5.664266e-50	3.135835e-47
MsG0580024748.01	MsG0880046885.01	0.819819	9.507833e-53	7.394955e-50
MsG0580024748.01	MsG0880046904.01	0.821801	3.327437e-53	2.734987e-50
MsG0580024748.01	MsG0880046905.01	0.824312	8.642600e-54	7.626954e-51
MsG0580024748.01	MsG0880046993.01	0.826875	2.133881e-54	2.026374e-51
MsG0580024748.01	MsG0880047447.01	0.824809	6.601599e-54	5.910702e-51
MsG0580024748.01	MsG0880047649.01	0.864241	1.401195e-64	4.369352e-61
MsG0380015058.01	MsG0580024748.01	0.806723	7.162462e-50	3.916071e-47
MsG0380015090.01	MsG0580024748.01	0.820245	7.595820e-53	5.978017e-50
MsG0380015219.01	MsG0580024748.01	0.814938	1.194176e-51	8.121258e-49
MsG0380015394.01	MsG0580024748.01	0.826323	2.890164e-54	2.701557e-51
MsG0380015736.01	MsG0580024748.01	0.838329	3.067697e-57	4.094901e-54
MsG0380016027.01	MsG0580024748.01	0.805894	1.070769e-49	5.731764e-47
MsG0380016225.01	MsG0580024748.01	0.846025	2.814524e-59	4.780465e-56
MsG0380016722.01	MsG0580024748.01	0.805358	1.387366e-49	7.324939e-47
MsG0380017164.01	MsG0580024748.01	0.804850	1.771790e-49	9.233736e-47
MsG0380017181.01	MsG0580024748.01	0.801858	7.383144e-49	3.563735e-46
MsG0380017282.01	MsG0580024748.01	0.831235	1.872304e-55	2.019473e-52
MsG0380017361.01	MsG0580024748.01	0.821226	4.518992e-53	3.654966e-50
MsG0380017440.01	MsG0580024748.01	0.844657	6.600707e-59	1.072954e-55
MsG0380017605.01	MsG0580024748.01	0.838916	2.163395e-57	2.940066e-54
MsG0380017632.01	MsG0580024748.01	0.828757	7.527797e-55	7.551429e-52
MsG0380018029.01	MsG0580024748.01	0.801177	1.018122e-48	4.830373e-46
MsG0480018397.01	MsG0580024748.01	0.825560	4.385871e-54	4.011430e-51
MsG0280006540.01	MsG0580024748.01	0.877707	5.062731e-69	2.571374e-65
MsG0280006563.01	MsG0580024748.01	0.851307	9.662955e-61	1.947746e-57
MsG0280007359.01	MsG0580024748.01	0.845953	2.943440e-59	4.987827e-56
MsG0280009752.01	MsG0580024748.01	0.831918	1.271031e-55	1.399214e-52
MsG0280009866.01	MsG0580024748.01	0.804253	2.361032e-49	1.211506e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024748.01.T02	MTR_5g015720	95.055	182	9	0	1	182	183	364	5.92e-113	330
MsG0580024748.01.T01	MTR_5g015720	94.702	151	6	1	1	151	1	149	1.07e-97	287

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024748.01.T02	AT5G67460	36.816	201	72	12	5	189	177	338	1.06e-14	72.8
MsG0580024748.01.T02	AT5G67460	36.816	201	72	12	5	189	177	338	1.08e-14	72.8
MsG0580024748.01.T01	AT5G67460	41.216	148	77	5	6	150	5	145	2.97e-20	85.9
MsG0580024748.01.T01	AT5G67460	41.216	148	77	5	6	150	5	145	3.43e-20	85.5

Find 77 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTAGTTTGCATTGCAGTTTC+TGG	0.188078	5:+9162901	MsG0580024748.01.T01:intron
TTGTTCTTGAGGGGCAAAAT+AGG	0.257528	5:-9163690	None:intergenic
CTTATGCTTTCAATAGTTAC+TGG	0.260747	5:+9163995	MsG0580024748.01.T01:three_prime_UTR
CCTTGCAATGCAATAGAAAA+TGG	0.301168	5:+9163779	MsG0580024748.01.T01:three_prime_UTR
GGTTGAGCAATTTCTGGACT+TGG	0.301603	5:-9163554	None:intergenic
GCAAAATAGGGAGAAGAAAT+TGG	0.319946	5:-9163677	None:intergenic
GAACAACATTAAGGTTGTTT+TGG	0.320840	5:-9163155	None:intergenic
TTCTTCTCACTGTCTTGTAT+TGG	0.334325	5:-9162645	None:intergenic
CATCTTCTCTTTCTTTGCTC+TGG	0.345679	5:+9162709	MsG0580024748.01.T01:CDS
TTTGAATTTGGTACATTGTA+AGG	0.345996	5:-9163614	None:intergenic
CCTGCTAACTCTCCTCAAAA+TGG	0.348344	5:+9163743	MsG0580024748.01.T01:three_prime_UTR
CACTTGTTTCACATAGGTTT+AGG	0.352249	5:-9162942	None:intergenic
ATGGTGATGGTTGTTCTTGA+GGG	0.357121	5:-9163700	None:intergenic
CAATGTTTATGATGTTGTTA+TGG	0.359003	5:-9163457	None:intergenic
GTTTGAAGAAACTCAATCAT+AGG	0.360523	5:-9163320	None:intergenic
TGAAGGTGGTGCTGATTGTT+TGG	0.361551	5:+9163916	MsG0580024748.01.T01:three_prime_UTR
GCATGAGCAACCAATGTATC+AGG	0.366680	5:-9163970	None:intergenic
ACAAGAAAAGAAAATGAAAA+TGG	0.371125	5:+9162675	MsG0580024748.01.T01:exon
TGTTCTTGAGGGGCAAAATA+GGG	0.397503	5:-9163689	None:intergenic
TGTAACGTTGCAAGAAGCTT+TGG	0.398217	5:+9163880	MsG0580024748.01.T01:three_prime_UTR
ACAGAAGTAGAACAACATTA+AGG	0.399975	5:-9163164	None:intergenic
GTTGAGCAATTTCTGGACTT+GGG	0.401126	5:-9163553	None:intergenic
GGTTGGAAGTAGCTGAGTTA+AGG	0.413965	5:-9163278	None:intergenic
TGGCAAAAGCATAAGAGAAT+TGG	0.415770	5:+9164015	MsG0580024748.01.T01:three_prime_UTR
CCTATGCAATACTCAGGTAT+AGG	0.422676	5:+9162591	None:intergenic
TTCTGAACAGGTGCAATTTG+TGG	0.425677	5:-9163824	None:intergenic
TTGCACCTGTTCAGAAACTT+TGG	0.430193	5:+9163831	MsG0580024748.01.T01:three_prime_UTR
TCTATCATTCCCTCAAAAGA+TGG	0.439369	5:+9163202	MsG0580024748.01.T01:CDS
GATGAAACTATTTGAGCTAA+AGG	0.452012	5:-9163647	None:intergenic
GTTGGAAGTAGCTGAGTTAA+GGG	0.454346	5:-9163277	None:intergenic
GCAATTTCTGGACTTGGGGT+TGG	0.457039	5:-9163548	None:intergenic
TATCAGCTGAAACTTGGCTT+AGG	0.457511	5:+9163055	MsG0580024748.01.T01:CDS
TTTGGTACATTGTAAGGAGC+TGG	0.460933	5:-9163608	None:intergenic
ATTTGAGCTAAAGGTGGTAA+AGG	0.464246	5:-9163638	None:intergenic
TTGGATTATGCTTGTGGTGA+AGG	0.471347	5:+9163899	MsG0580024748.01.T01:three_prime_UTR
AATGGTGATGGTTGTTCTTG+AGG	0.476899	5:-9163701	None:intergenic
ATTGGTGGAACTTGTGACTT+TGG	0.478733	5:+9164033	MsG0580024748.01.T01:three_prime_UTR
ACACACCAAAGTTTCTGAAC+AGG	0.478993	5:-9163836	None:intergenic
TTGTTACAATCCTGATACAT+TGG	0.480675	5:+9163960	MsG0580024748.01.T01:three_prime_UTR
CTATCATTCCCTCAAAAGAT+GGG	0.481726	5:+9163203	MsG0580024748.01.T01:CDS
GTGGAACTTGTGACTTTGGT+GGG	0.482171	5:+9164037	MsG0580024748.01.T01:three_prime_UTR
TGTTGCTAGAGACCTTGTTA+AGG	0.501164	5:-9163029	None:intergenic
ACATTGTAAGGAGCTGGATA+AGG	0.502108	5:-9163602	None:intergenic
GCTTCTTGCAACGTTACATC+AGG	0.504907	5:-9163875	None:intergenic
CAAAAGCATAAGAGAATTGG+TGG	0.510123	5:+9164018	MsG0580024748.01.T01:three_prime_UTR
AAATGTGAAAACATTGAACT+TGG	0.513995	5:-9163368	None:intergenic
TTGAGCAATTTCTGGACTTG+GGG	0.516364	5:-9163552	None:intergenic
GATACAAACTGTGGTGGTAA+TGG	0.537334	5:-9163719	None:intergenic
AACCAGCAGGGAAATCCAAA+GGG	0.537740	5:-9163577	None:intergenic
TAGGGAGAAGAAATTGGTGG+TGG	0.538044	5:-9163671	None:intergenic
GAACCAGCAGGGAAATCCAA+AGG	0.539386	5:-9163578	None:intergenic
GCTGGATAAGGAGAACCAGC+AGG	0.554077	5:-9163590	None:intergenic
TGTCTTCACTTGTTTCACAT+AGG	0.568580	5:-9162948	None:intergenic
ACAGTCCAAATCAAAAGCAA+TGG	0.570988	5:-9163250	None:intergenic
CTGTTCAAAGAAACAGCTAA+AGG	0.580124	5:-9163000	None:intergenic
GATTATGCTTGTGGTGAAGG+TGG	0.582581	5:+9163902	MsG0580024748.01.T01:three_prime_UTR
GGTGGAACTTGTGACTTTGG+TGG	0.584114	5:+9164036	MsG0580024748.01.T01:three_prime_UTR
CAAGACAGTGAGAAGAACCT+TGG	0.600657	5:+9162651	MsG0580024748.01.T01:five_prime_UTR
GAAGCTTTGGATTATGCTTG+TGG	0.604890	5:+9163893	MsG0580024748.01.T01:three_prime_UTR
TTGGAGATTACTACTCCACA+AGG	0.630522	5:+9163935	MsG0580024748.01.T01:three_prime_UTR
AAAAGAAAATGAAAATGGTG+TGG	0.631353	5:+9162680	MsG0580024748.01.T01:exon
AACGTTACATCAGGAACACT+AGG	0.631354	5:-9163866	None:intergenic
TATCATTCCCTCAAAAGATG+GGG	0.632855	5:+9163204	MsG0580024748.01.T01:CDS
AACTGTGGTGGTAATGGTGA+TGG	0.632946	5:-9163713	None:intergenic
GAAACTATTTGAGCTAAAGG+TGG	0.639226	5:-9163644	None:intergenic
TGGTGATGGTTGTTCTTGAG+GGG	0.643729	5:-9163699	None:intergenic
AGGATTGTAACAATTCCTTG+TGG	0.647642	5:-9163950	None:intergenic
AAATAGGGAGAAGAAATTGG+TGG	0.650191	5:-9163674	None:intergenic
GAACAGTGAAGACCTTAACA+AGG	0.657274	5:+9163017	MsG0580024748.01.T01:CDS
GCAATTTGTGGATAATGTGA+AGG	0.661579	5:-9163812	None:intergenic
TGTGGATAATGTGAAGGTGA+AGG	0.662098	5:-9163806	None:intergenic
CTGGATAAGGAGAACCAGCA+GGG	0.668605	5:-9163589	None:intergenic
AATACTCAGGTATAGGACAA+AGG	0.669485	5:+9162598	None:intergenic
GCAGGAGATACAAACTGTGG+TGG	0.675527	5:-9163725	None:intergenic
ACCAGCAGGGAAATCCAAAG+GGG	0.725497	5:-9163576	None:intergenic
CCAGCAGGGAAATCCAAAGG+GGG	0.739134	5:-9163575	None:intergenic
TTAGCAGGAGATACAAACTG+TGG	0.759496	5:-9163728	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATGTATAAACTTATATTATG+TGG	+	Chr5:9164569-9164588	MsG0580024748.01.T01:three_prime_UTR	15.0%
!!!	ATGTCTTAATTTTTGTTATT+AGG	+	Chr5:9164610-9164629	MsG0580024748.01.T01:three_prime_UTR	15.0%
!!!	TAATTTTTGTTATTAGGATT+TGG	+	Chr5:9164616-9164635	MsG0580024748.01.T01:three_prime_UTR	15.0%
!!!	TTTTGTTGTTTCTTATATAT+AGG	-	Chr5:9162625-9162644	None:intergenic	15.0%
!!	AAGTAAGTGCTTATTAATTT+AGG	+	Chr5:9164086-9164105	MsG0580024748.01.T01:intron	20.0%
!!	ACAAGAAAAGAAAATGAAAA+TGG	+	Chr5:9162675-9162694	MsG0580024748.01.T01:exon	20.0%
!!	TAAATTAATAAGCACTTACT+TGG	-	Chr5:9164087-9164106	None:intergenic	20.0%
!!	TGTTAATTAATAATGAGTGA+TGG	+	Chr5:9164136-9164155	MsG0580024748.01.T01:intron	20.0%
!!	TTACATTAATAATGGTGAAT+TGG	+	Chr5:9164372-9164391	MsG0580024748.01.T01:intron	20.0%
!!!	AATTAATTAGCTGTTTTAGA+TGG	-	Chr5:9164321-9164340	None:intergenic	20.0%
!!!	GGTTTTAAATTTTCAAATCA+TGG	-	Chr5:9163302-9163321	None:intergenic	20.0%
!!!	TTAAATTTTCAAATCATGGT+TGG	-	Chr5:9163298-9163317	None:intergenic	20.0%
!	AAAAGAAAATGAAAATGGTG+TGG	+	Chr5:9162680-9162699	MsG0580024748.01.T01:exon	25.0%
!	AAACTAATAATAGAAACCAC+AGG	-	Chr5:9164470-9164489	None:intergenic	25.0%
!	AAATGTGAAAACATTGAACT+TGG	-	Chr5:9163371-9163390	None:intergenic	25.0%
!	AAGACATAAATTCTCCAAAT+TGG	-	Chr5:9164597-9164616	None:intergenic	25.0%
!	AGACATAAATTCTCCAAATT+GGG	-	Chr5:9164596-9164615	None:intergenic	25.0%
!	CAATGTTTATGATGTTGTTA+TGG	-	Chr5:9163460-9163479	None:intergenic	25.0%
!	TATAGTTATTGTACATAGCA+GGG	-	Chr5:9164251-9164270	None:intergenic	25.0%
!	TTATAGTTATTGTACATAGC+AGG	-	Chr5:9164252-9164271	None:intergenic	25.0%
!	TTTGAATTTGGTACATTGTA+AGG	-	Chr5:9163617-9163636	None:intergenic	25.0%
!!	ATCACTAATCAGAATTTTGA+AGG	-	Chr5:9164676-9164695	None:intergenic	25.0%
!!	ATTTTTGATGAAGGGTAATA+TGG	-	Chr5:9163093-9163112	None:intergenic	25.0%
!!	TAGTGATTTTTCTCTCTTTT+GGG	-	Chr5:9163493-9163512	None:intergenic	25.0%
!!	TCACTAATCAGAATTTTGAA+GGG	-	Chr5:9164675-9164694	None:intergenic	25.0%
!!	TTTTTGATGAAGGGTAATAT+GGG	-	Chr5:9163092-9163111	None:intergenic	25.0%
!!!	CTTTTTCATGGATTCTAAAT+GGG	-	Chr5:9163423-9163442	None:intergenic	25.0%
	AACCAAAGTGATCTTAACAT+AGG	-	Chr5:9164514-9164533	None:intergenic	30.0%
	ACAGAAGTAGAACAACATTA+AGG	-	Chr5:9163167-9163186	None:intergenic	30.0%
	ATAAAGTATGCAACTACCAA+AGG	+	Chr5:9164268-9164287	MsG0580024748.01.T01:intron	30.0%
	ATCCTATGTTAAGATCACTT+TGG	+	Chr5:9164509-9164528	MsG0580024748.01.T01:three_prime_UTR	30.0%
	CTTATGCTTTCAATAGTTAC+TGG	+	Chr5:9163995-9164014	MsG0580024748.01.T01:three_prime_UTR	30.0%
	GATGAAACTATTTGAGCTAA+AGG	-	Chr5:9163650-9163669	None:intergenic	30.0%
	GTTTGAAGAAACTCAATCAT+AGG	-	Chr5:9163323-9163342	None:intergenic	30.0%
	TATATTATGTGGACCCAATT+TGG	+	Chr5:9164580-9164599	MsG0580024748.01.T01:three_prime_UTR	30.0%
	TCAAAAATCACCAACATTGT+TGG	+	Chr5:9163105-9163124	MsG0580024748.01.T01:CDS	30.0%
	TTAGAATCCATGAAAAAGCT+TGG	+	Chr5:9163425-9163444	MsG0580024748.01.T01:three_prime_UTR	30.0%
	TTGTTACAATCCTGATACAT+TGG	+	Chr5:9163960-9163979	MsG0580024748.01.T01:three_prime_UTR	30.0%
!	ACATTTTATCAGCTGAAACT+TGG	+	Chr5:9163049-9163068	MsG0580024748.01.T01:CDS	30.0%
!	AGTTAGTTACACAGTTTGTT+AGG	-	Chr5:9164294-9164313	None:intergenic	30.0%
!	GAACAACATTAAGGTTGTTT+TGG	-	Chr5:9163158-9163177	None:intergenic	30.0%
!	GTAGTGATTTTTCTCTCTTT+TGG	-	Chr5:9163494-9163513	None:intergenic	30.0%
!	TGAAGTTTTTCCAACAATGT+TGG	-	Chr5:9163118-9163137	None:intergenic	30.0%
!!	AGTTTCCATTGCTTTTGATT+TGG	+	Chr5:9163245-9163264	MsG0580024748.01.T01:CDS	30.0%
!!	ATTGGTTATTGTTTTGTTGC+AGG	+	Chr5:9164390-9164409	MsG0580024748.01.T01:intron	30.0%
!!!	ATGTTGGTGATTTTTGATGA+AGG	-	Chr5:9163102-9163121	None:intergenic	30.0%
!!!	GAAAAATCCAAGCTTTTTCA+TGG	-	Chr5:9163435-9163454	None:intergenic	30.0%
!!!	GCTTTTTCATGGATTCTAAA+TGG	-	Chr5:9163424-9163443	None:intergenic	30.0%
!!!	TGTTGGTGATTTTTGATGAA+GGG	-	Chr5:9163101-9163120	None:intergenic	30.0%
!!!	TTTTCTCTCTTTTGGGATAA+TGG	-	Chr5:9163486-9163505	None:intergenic	30.0%
	AAATAGGGAGAAGAAATTGG+TGG	-	Chr5:9163677-9163696	None:intergenic	35.0%
	ACAGTCCAAATCAAAAGCAA+TGG	-	Chr5:9163253-9163272	None:intergenic	35.0%
	AGATCACTTTGGTTGTTGAT+TGG	+	Chr5:9164520-9164539	MsG0580024748.01.T01:three_prime_UTR	35.0%
	AGGATTGTAACAATTCCTTG+TGG	-	Chr5:9163953-9163972	None:intergenic	35.0%
	ATTTGAGCTAAAGGTGGTAA+AGG	-	Chr5:9163641-9163660	None:intergenic	35.0%
	CACTTGTTTCACATAGGTTT+AGG	-	Chr5:9162945-9162964	None:intergenic	35.0%
	CCTTGCAATGCAATAGAAAA+TGG	+	Chr5:9163779-9163798	MsG0580024748.01.T01:three_prime_UTR	35.0%
	CTATCATTCCCTCAAAAGAT+GGG	+	Chr5:9163203-9163222	MsG0580024748.01.T01:CDS	35.0%
	CTGTTCAAAGAAACAGCTAA+AGG	-	Chr5:9163003-9163022	None:intergenic	35.0%
	GAAACTATTTGAGCTAAAGG+TGG	-	Chr5:9163647-9163666	None:intergenic	35.0%
	GCAAAATAGGGAGAAGAAAT+TGG	-	Chr5:9163680-9163699	None:intergenic	35.0%
	GCAATTTGTGGATAATGTGA+AGG	-	Chr5:9163815-9163834	None:intergenic	35.0%
	TATCATTCCCTCAAAAGATG+GGG	+	Chr5:9163204-9163223	MsG0580024748.01.T01:CDS	35.0%
	TCTATCATTCCCTCAAAAGA+TGG	+	Chr5:9163202-9163221	MsG0580024748.01.T01:CDS	35.0%
	TGAGAATAAACCTACAGTGA+AGG	-	Chr5:9164419-9164438	None:intergenic	35.0%
	TGTCTTCACTTGTTTCACAT+AGG	-	Chr5:9162951-9162970	None:intergenic	35.0%
	TTCTTCTCACTGTCTTGTAT+TGG	-	Chr5:9162648-9162667	None:intergenic	35.0%
!	CAAAAGCATAAGAGAATTGG+TGG	+	Chr5:9164018-9164037	MsG0580024748.01.T01:three_prime_UTR	35.0%
!	CATTTTCTTTTCTTGTGCCA+AGG	-	Chr5:9162671-9162690	None:intergenic	35.0%
!	CCATTTTCTATTGCATTGCA+AGG	-	Chr5:9163782-9163801	None:intergenic	35.0%
!	TGGCAAAAGCATAAGAGAAT+TGG	+	Chr5:9164015-9164034	MsG0580024748.01.T01:three_prime_UTR	35.0%
!	TTCTAAACCCCATCTTTTGA+GGG	-	Chr5:9163214-9163233	None:intergenic	35.0%
!	TTTTCTATTGCATTGCAAGG+TGG	-	Chr5:9163779-9163798	None:intergenic	35.0%
!!!	GGTGGTAAAGGTTTTGAATT+TGG	-	Chr5:9163629-9163648	None:intergenic	35.0%
!!!	GGTTGGTTTTATAGGAATTG+TGG	-	Chr5:9163534-9163553	None:intergenic	35.0%
!!!	TAAGGTTGTTTTGGTCTTGT+TGG	-	Chr5:9163149-9163168	None:intergenic	35.0%
	AACGTTACATCAGGAACACT+AGG	-	Chr5:9163869-9163888	None:intergenic	40.0%
	ACACACCAAAGTTTCTGAAC+AGG	-	Chr5:9163839-9163858	None:intergenic	40.0%
	ACATTGTAAGGAGCTGGATA+AGG	-	Chr5:9163605-9163624	None:intergenic	40.0%
	AGTGATGGTTGTGTTGTGAA+TGG	+	Chr5:9164151-9164170	MsG0580024748.01.T01:intron	40.0%
	ATTGGTGGAACTTGTGACTT+TGG	+	Chr5:9164033-9164052	MsG0580024748.01.T01:three_prime_UTR	40.0%
	CATCTTCTCTTTCTTTGCTC+TGG	+	Chr5:9162709-9162728	MsG0580024748.01.T01:CDS	40.0%
	GAACAGTGAAGACCTTAACA+AGG	+	Chr5:9163017-9163036	MsG0580024748.01.T01:CDS	40.0%
	GATACAAACTGTGGTGGTAA+TGG	-	Chr5:9163722-9163741	None:intergenic	40.0%
	GTTGGAAGTAGCTGAGTTAA+GGG	-	Chr5:9163280-9163299	None:intergenic	40.0%
	TATCAGCTGAAACTTGGCTT+AGG	+	Chr5:9163055-9163074	MsG0580024748.01.T01:CDS	40.0%
	TGTAACGTTGCAAGAAGCTT+TGG	+	Chr5:9163880-9163899	MsG0580024748.01.T01:three_prime_UTR	40.0%
	TGTGGATAATGTGAAGGTGA+AGG	-	Chr5:9163809-9163828	None:intergenic	40.0%
	TGTTCTTGAGGGGCAAAATA+GGG	-	Chr5:9163692-9163711	None:intergenic	40.0%
	TGTTGCTAGAGACCTTGTTA+AGG	-	Chr5:9163032-9163051	None:intergenic	40.0%
	TTAGCAGGAGATACAAACTG+TGG	-	Chr5:9163731-9163750	None:intergenic	40.0%
	TTCTGAACAGGTGCAATTTG+TGG	-	Chr5:9163827-9163846	None:intergenic	40.0%
	TTGCACCTGTTCAGAAACTT+TGG	+	Chr5:9163831-9163850	MsG0580024748.01.T01:three_prime_UTR	40.0%
	TTGGAGATTACTACTCCACA+AGG	+	Chr5:9163935-9163954	MsG0580024748.01.T01:three_prime_UTR	40.0%
	TTGGATTATGCTTGTGGTGA+AGG	+	Chr5:9163899-9163918	MsG0580024748.01.T01:three_prime_UTR	40.0%
	TTGTTCTTGAGGGGCAAAAT+AGG	-	Chr5:9163693-9163712	None:intergenic	40.0%
	TTTGGTACATTGTAAGGAGC+TGG	-	Chr5:9163611-9163630	None:intergenic	40.0%
!	GGACAAGTGAAACCATTTTG+AGG	-	Chr5:9163758-9163777	None:intergenic	40.0%
!	GTAGTTTGCATTGCAGTTTC+TGG	+	Chr5:9162901-9162920	MsG0580024748.01.T01:intron	40.0%
!	GTTGAGCAATTTCTGGACTT+GGG	-	Chr5:9163556-9163575	None:intergenic	40.0%
!	TTGAGCAATTTCTGGACTTG+GGG	-	Chr5:9163555-9163574	None:intergenic	40.0%
!	TTTTGCTACTAGCTAGCTAG+TGG	+	Chr5:9164543-9164562	MsG0580024748.01.T01:three_prime_UTR	40.0%
!!	AATGGTGATGGTTGTTCTTG+AGG	-	Chr5:9163704-9163723	None:intergenic	40.0%
!!	ATGGTGATGGTTGTTCTTGA+GGG	-	Chr5:9163703-9163722	None:intergenic	40.0%
!!	GAAGCTTTGGATTATGCTTG+TGG	+	Chr5:9163893-9163912	MsG0580024748.01.T01:three_prime_UTR	40.0%
!!	GTTCTAAACCCCATCTTTTG+AGG	-	Chr5:9163215-9163234	None:intergenic	40.0%
!!	TACACAGTTTGTTAGGCCTT+TGG	-	Chr5:9164287-9164306	None:intergenic	40.0%
	AAAGGGGGTTGAGCAATTTC+TGG	-	Chr5:9163563-9163582	None:intergenic	45.0%
	AACCAGCAGGGAAATCCAAA+GGG	-	Chr5:9163580-9163599	None:intergenic	45.0%
	AGAAATTGCTCAACCCCCTT+TGG	+	Chr5:9163562-9163581	MsG0580024748.01.T01:three_prime_UTR	45.0%
	CAAGACAGTGAGAAGAACCT+TGG	+	Chr5:9162651-9162670	MsG0580024748.01.T01:five_prime_UTR	45.0%
	CCTGCTAACTCTCCTCAAAA+TGG	+	Chr5:9163743-9163762	MsG0580024748.01.T01:three_prime_UTR	45.0%
	GATTATGCTTGTGGTGAAGG+TGG	+	Chr5:9163902-9163921	MsG0580024748.01.T01:three_prime_UTR	45.0%
	GCATGAGCAACCAATGTATC+AGG	-	Chr5:9163973-9163992	None:intergenic	45.0%
	GCTTCTTGCAACGTTACATC+AGG	-	Chr5:9163878-9163897	None:intergenic	45.0%
	GGTTGGAAGTAGCTGAGTTA+AGG	-	Chr5:9163281-9163300	None:intergenic	45.0%
	GTGGAACTTGTGACTTTGGT+GGG	+	Chr5:9164037-9164056	MsG0580024748.01.T01:three_prime_UTR	45.0%
	TAGGGAGAAGAAATTGGTGG+TGG	-	Chr5:9163674-9163693	None:intergenic	45.0%
	TGCATGAAGAGTTGTTCCTG+TGG	+	Chr5:9164451-9164470	MsG0580024748.01.T01:three_prime_UTR	45.0%
	TTGCAGGTTTCCTTCACTGT+AGG	+	Chr5:9164406-9164425	MsG0580024748.01.T01:intron	45.0%
!	GGTTGAGCAATTTCTGGACT+TGG	-	Chr5:9163557-9163576	None:intergenic	45.0%
!!	AACTGTGGTGGTAATGGTGA+TGG	-	Chr5:9163716-9163735	None:intergenic	45.0%
!!	CCATTTTGAGGAGAGTTAGC+AGG	-	Chr5:9163746-9163765	None:intergenic	45.0%
!!	TGAAGGTGGTGCTGATTGTT+TGG	+	Chr5:9163916-9163935	MsG0580024748.01.T01:three_prime_UTR	45.0%
!!	TGGTGATGGTTGTTCTTGAG+GGG	-	Chr5:9163702-9163721	None:intergenic	45.0%
!!!	GGACTTGGGGTTGGTTTTAT+AGG	-	Chr5:9163542-9163561	None:intergenic	45.0%
!!	TTATTTATTTACATTAATAA+TGG	+	Chr5:9164364-9164383	MsG0580024748.01.T01:intron	5.0%
	ACCAGCAGGGAAATCCAAAG+GGG	-	Chr5:9163579-9163598	None:intergenic	50.0%
	CTGGATAAGGAGAACCAGCA+GGG	-	Chr5:9163592-9163611	None:intergenic	50.0%
	GAACCAGCAGGGAAATCCAA+AGG	-	Chr5:9163581-9163600	None:intergenic	50.0%
	GCAGGAGATACAAACTGTGG+TGG	-	Chr5:9163728-9163747	None:intergenic	50.0%
	GGTGGAACTTGTGACTTTGG+TGG	+	Chr5:9164036-9164055	MsG0580024748.01.T01:three_prime_UTR	50.0%
!	GCAATTTCTGGACTTGGGGT+TGG	-	Chr5:9163551-9163570	None:intergenic	50.0%
	CCAGCAGGGAAATCCAAAGG+GGG	-	Chr5:9163578-9163597	None:intergenic	55.0%
	GCTGGATAAGGAGAACCAGC+AGG	-	Chr5:9163593-9163612	None:intergenic	55.0%
	CCCCCTTTGGATTTCCCTGC+TGG	+	Chr5:9163575-9163594	MsG0580024748.01.T01:three_prime_UTR	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	9162601	9164707	9162601	ID=MsG0580024748.01;Name=MsG0580024748.01
Chr5	mRNA	9162601	9164707	9162601	ID=MsG0580024748.01.T01;Parent=MsG0580024748.01;Name=MsG0580024748.01.T01;_AED=0.12;_eAED=0.12;_QI=87\|1\|1\|1\|0\|0\|3\|1066\|151
Chr5	exon	9162601	9162730	9162601	ID=MsG0580024748.01.T01:exon:26413;Parent=MsG0580024748.01.T01
Chr5	exon	9162905	9164087	9162905	ID=MsG0580024748.01.T01:exon:26414;Parent=MsG0580024748.01.T01
Chr5	five_prime_UTR	9162601	9162687	9162601	ID=MsG0580024748.01.T01:five_prime_utr;Parent=MsG0580024748.01.T01
Chr5	CDS	9162688	9162730	9162688	ID=MsG0580024748.01.T01:cds;Parent=MsG0580024748.01.T01
Chr5	CDS	9162905	9163317	9162905	ID=MsG0580024748.01.T01:cds;Parent=MsG0580024748.01.T01
Chr5	three_prime_UTR	9163318	9164087	9163318	ID=MsG0580024748.01.T01:three_prime_utr;Parent=MsG0580024748.01.T01
Chr5	three_prime_UTR	9164412	9164707	9164412	ID=MsG0580024748.01.T01:three_prime_utr;Parent=MsG0580024748.01.T01
Chr5	mRNA	9163413	9164707	9163413	ID=MsG0580024748.01.T02;Parent=MsG0580024748.01;Name=MsG0580024748.01.T02;_AED=0.28;_eAED=0.35;_QI=0\|0\|0.5\|1\|0\|0\|2\|221\|238
Chr5	exon	9163413	9164054	9163413	ID=MsG0580024748.01.T02:exon:26416;Parent=MsG0580024748.01.T02
Chr5	CDS	9163413	9164054	9163413	ID=MsG0580024748.01.T02:cds;Parent=MsG0580024748.01.T02
Chr5	CDS	9164412	9164486	9164412	ID=MsG0580024748.01.T02:cds;Parent=MsG0580024748.01.T02
Chr5	three_prime_UTR	9164487	9164707	9164487	ID=MsG0580024748.01.T02:three_prime_utr;Parent=MsG0580024748.01.T02

Gene Sequence

>MsG0580024748.01.T02

ATGTCTTCCCATTTAGAATCCATGAAAAAGCTTGGATTTTTCAACCATAACAACATCATAAACATTGCTACCATTATCCCAAAAGAGAGAAAAATCACTACTAGAAAACTTTCAGTTACCACAATTCCTATAAAACCAACCCCAAGTCCAGAAATTGCTCAACCCCCTTTGGATTTCCCTGCTGGTTCTCCTTATCCAGCTCCTTACAATGTACCAAATTCAAAACCTTTACCACCTTTAGCTCAAATAGTTTCATCTCCACCACCAATTTCTTCTCCCTATTTTGCCCCTCAAGAACAACCATCACCATTACCACCACAGTTTGTATCTCCTGCTAACTCTCCTCAAAATGGTTTCACTTGTCCACCTTGCAATGCAATAGAAAATGGTTCACCTTCACCTTCACATTATCCACAAATTGCACCTGTTCAGAAACTTTGGTGTGTTGCAAAGCCTAGTGTTCCTGATGTAACGTTGCAAGAAGCTTTGGATTATGCTTGTGGTGAAGGTGGTGCTGATTGTTTGGAGATTACTACTCCACAAGGAATTGTTACAATCCTGATACATTGGTTGCTCATGCTTCTTATGCTTTCAATAGTTACTGGCAAAAGCATAAGAGAATTGGTGGAACTTGTGACTTTGGTTTCCTTCACTGTAGGTTTATTCTCAGCTAGATTCAAGTGCATGAAGAGTTGTTCCTGTGGTTTCTATTATTAG

>MsG0580024748.01.T01

ATGAAAATGGTGTGGCTATTTCATCTTCTCTTTCTTTGCTCTGTTTGCATTGCAGTTTCTGGTCAACAAAACATTGAGTTCCTAAACCTATGTGAAACAAGTGAAGACATTGCAACATCTTCATCTCACAGTGAGTTTCCTTTAGCTGTTTCTTTGAACAGTGAAGACCTTAACAAGGTCTCTAGCAACATTTTATCAGCTGAAACTTGGCTTAGGTTAAACATTTTCCCATATTACCCTTCATCAAAAATCACCAACATTGTTGGAAAAACTTCAACTTTCTGCCAACAAGACCAAAACAACCTTAATGTTGTTCTACTTCTGTCTTCTTTGAAAAATCTCTATCATTCCCTCAAAAGATGGGGTTTAGAACAAGACATCAAAGTTTCCATTGCTTTTGATTTGGACTGTTTTACCCTTAACTCAGCTACTTCCAACCATGATTTGAAAATTTAA

Protein sequence

>MsG0580024748.01.T02

MSSHLESMKKLGFFNHNNIINIATIIPKERKITTRKLSVTTIPIKPTPSPEIAQPPLDFPAGSPYPAPYNVPNSKPLPPLAQIVSSPPPISSPYFAPQEQPSPLPPQFVSPANSPQNGFTCPPCNAIENGSPSPSHYPQIAPVQKLWCVAKPSVPDVTLQEALDYACGEGGADCLEITTPQGIVTILIHWLLMLLMLSIVTGKSIRELVELVTLVSFTVGLFSARFKCMKSCSCGFYY*

>MsG0580024748.01.T01

MKMVWLFHLLFLCSVCIAVSGQQNIEFLNLCETSEDIATSSSHSEFPLAVSLNSEDLNKVSSNILSAETWLRLNIFPYYPSSKITNIVGKTSTFCQQDQNNLNVVLLLSSLKNLYHSLKRWGLEQDIKVSIAFDLDCFTLNSATSNHDLKI*