AlfalfaGEDB | MsG0580024748.01

M. sativa cultivar ZhongmuNo.1 / MsG0580024748.01

KEGG: -
GO: -
PFAM: PF07983

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024748.01.T01	XP_003611601.1	94.702	151	6	1	1	151	1	149	8.79E-94	287
MsG0580024748.01.T02	AFK38186.1	95.055	182	9	0	1	182	183	364	4.73E-109	330

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024748.01.T01	G7K3T9	94.702	151	6	1	1	151	1	149	4.20e-94	287
MsG0580024748.01.T02	I3SD44	95.055	182	9	0	1	182	183	364	2.26e-109	330

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047815.01	MsG0580024748.01	0.805102	1.569890e-49	8.234113e-47
MsG0080048258.01	MsG0580024748.01	0.808525	2.968010e-50	1.700927e-47
MsG0080048775.01	MsG0580024748.01	0.825336	4.954754e-54	4.503245e-51
MsG0080048781.01	MsG0580024748.01	0.819082	1.400062e-52	1.066790e-49
MsG0080049140.01	MsG0580024748.01	0.821402	4.116053e-53	3.345252e-50
MsG0180000111.01	MsG0580024748.01	0.815421	9.325228e-52	6.426295e-49
MsG0180000314.01	MsG0580024748.01	0.807482	4.946197e-50	2.758178e-47
MsG0180002218.01	MsG0580024748.01	0.807816	4.201977e-50	2.363542e-47
MsG0180002397.01	MsG0580024748.01	0.841923	3.538458e-58	5.278562e-55
MsG0180002583.01	MsG0580024748.01	0.827669	1.377163e-54	1.338419e-51

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024748.01.T01	MTR_5g015720	94.702	151	6	1	1	151	1	149	1.07e-97	287
MsG0580024748.01.T02	MTR_5g015720	95.055	182	9	0	1	182	183	364	5.92e-113	330

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024748.01.T01	AT5G67460	41.216	148	77	5	6	150	5	145	2.97e-20	85.9
MsG0580024748.01.T01	AT5G67460	41.216	148	77	5	6	150	5	145	3.43e-20	85.5
MsG0580024748.01.T02	AT5G67460	36.816	201	72	12	5	189	177	338	1.06e-14	72.8
MsG0580024748.01.T02	AT5G67460	36.816	201	72	12	5	189	177	338	1.08e-14	72.8

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Find 77 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAAATGAAAATGGTG+TGG	0.631353	5:+9162680	MsG0580024748.01.T01:exon
AAATAGGGAGAAGAAATTGG+TGG	0.650191	5:-9163674	None:intergenic
AAATGTGAAAACATTGAACT+TGG	0.513995	5:-9163368	None:intergenic
AACCAGCAGGGAAATCCAAA+GGG	0.537740	5:-9163577	None:intergenic
AACGTTACATCAGGAACACT+AGG	0.631354	5:-9163866	None:intergenic
AACTGTGGTGGTAATGGTGA+TGG	0.632946	5:-9163713	None:intergenic
AATACTCAGGTATAGGACAA+AGG	0.669485	5:+9162598	None:intergenic
AATGGTGATGGTTGTTCTTG+AGG	0.476899	5:-9163701	None:intergenic
ACAAGAAAAGAAAATGAAAA+TGG	0.371125	5:+9162675	MsG0580024748.01.T01:exon
ACACACCAAAGTTTCTGAAC+AGG	0.478993	5:-9163836	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACCAAAGTGATCTTAACAT+AGG	-	Chr5:9164514-9164533	None:intergenic	30.0%
ACAGAAGTAGAACAACATTA+AGG	-	Chr5:9163167-9163186	None:intergenic	30.0%
ATAAAGTATGCAACTACCAA+AGG	+	Chr5:9164268-9164287	MsG0580024748.01.T01:intron	30.0%
ATCCTATGTTAAGATCACTT+TGG	+	Chr5:9164509-9164528	MsG0580024748.01.T01:three_prime_UTR	30.0%
CTTATGCTTTCAATAGTTAC+TGG	+	Chr5:9163995-9164014	MsG0580024748.01.T01:three_prime_UTR	30.0%
GATGAAACTATTTGAGCTAA+AGG	-	Chr5:9163650-9163669	None:intergenic	30.0%
GTTTGAAGAAACTCAATCAT+AGG	-	Chr5:9163323-9163342	None:intergenic	30.0%
TATATTATGTGGACCCAATT+TGG	+	Chr5:9164580-9164599	MsG0580024748.01.T01:three_prime_UTR	30.0%
TCAAAAATCACCAACATTGT+TGG	+	Chr5:9163105-9163124	MsG0580024748.01.T01:CDS	30.0%
TTAGAATCCATGAAAAAGCT+TGG	+	Chr5:9163425-9163444	MsG0580024748.01.T01:three_prime_UTR	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	9162601	9164707	9162601	ID=MsG0580024748.01;Name=MsG0580024748.01
Chr5	mRNA	9162601	9164707	9162601	ID=MsG0580024748.01.T01;Parent=MsG0580024748.01;Name=MsG0580024748.01.T01;_AED=0.12;_eAED=0.12;_QI=87\|1\|1\|1\|0\|0\|3\|1066\|151
Chr5	exon	9162601	9162730	9162601	ID=MsG0580024748.01.T01:exon:26413;Parent=MsG0580024748.01.T01
Chr5	exon	9162905	9164087	9162905	ID=MsG0580024748.01.T01:exon:26414;Parent=MsG0580024748.01.T01
Chr5	five_prime_UTR	9162601	9162687	9162601	ID=MsG0580024748.01.T01:five_prime_utr;Parent=MsG0580024748.01.T01
Chr5	CDS	9162688	9162730	9162688	ID=MsG0580024748.01.T01:cds;Parent=MsG0580024748.01.T01
Chr5	CDS	9162905	9163317	9162905	ID=MsG0580024748.01.T01:cds;Parent=MsG0580024748.01.T01
Chr5	three_prime_UTR	9163318	9164087	9163318	ID=MsG0580024748.01.T01:three_prime_utr;Parent=MsG0580024748.01.T01
Chr5	three_prime_UTR	9164412	9164707	9164412	ID=MsG0580024748.01.T01:three_prime_utr;Parent=MsG0580024748.01.T01
Chr5	mRNA	9163413	9164707	9163413	ID=MsG0580024748.01.T02;Parent=MsG0580024748.01;Name=MsG0580024748.01.T02;_AED=0.28;_eAED=0.35;_QI=0\|0\|0.5\|1\|0\|0\|2\|221\|238
Chr5	exon	9163413	9164054	9163413	ID=MsG0580024748.01.T02:exon:26416;Parent=MsG0580024748.01.T02
Chr5	CDS	9163413	9164054	9163413	ID=MsG0580024748.01.T02:cds;Parent=MsG0580024748.01.T02
Chr5	CDS	9164412	9164486	9164412	ID=MsG0580024748.01.T02:cds;Parent=MsG0580024748.01.T02
Chr5	three_prime_UTR	9164487	9164707	9164487	ID=MsG0580024748.01.T02:three_prime_utr;Parent=MsG0580024748.01.T02

Gene Sequence

>MsG0580024748.01.T02

ATGTCTTCCCATTTAGAATCCATGAAAAAGCTTGGATTTTTCAACCATAACAACATCATAAACATTGCTACCATTATCCCAAAAGAGAGAAAAATCACTACTAGAAAACTTTCAGTTACCACAATTCCTATAAAACCAACCCCAAGTCCAGAAATTGCTCAACCCCCTTTGGATTTCCCTGCTGGTTCTCCTTATCCAGCTCCTTACAATGTACCAAATTCAAAACCTTTACCACCTTTAGCTCAAATAGTTTCATCTCCACCACCAATTTCTTCTCCCTATTTTGCCCCTCAAGAACAACCATCACCATTACCACCACAGTTTGTATCTCCTGCTAACTCTCCTCAAAATGGTTTCACTTGTCCACCTTGCAATGCAATAGAAAATGGTTCACCTTCACCTTCACATTATCCACAAATTGCACCTGTTCAGAAACTTTGGTGTGTTGCAAAGCCTAGTGTTCCTGATGTAACGTTGCAAGAAGCTTTGGATTATGCTTGTGGTGAAGGTGGTGCTGATTGTTTGGAGATTACTACTCCACAAGGAATTGTTACAATCCTGATACATTGGTTGCTCATGCTTCTTATGCTTTCAATAGTTACTGGCAAAAGCATAAGAGAATTGGTGGAACTTGTGACTTTGGTTTCCTTCACTGTAGGTTTATTCTCAGCTAGATTCAAGTGCATGAAGAGTTGTTCCTGTGGTTTCTATTATTAG

>MsG0580024748.01.T01

ATGAAAATGGTGTGGCTATTTCATCTTCTCTTTCTTTGCTCTGTTTGCATTGCAGTTTCTGGTCAACAAAACATTGAGTTCCTAAACCTATGTGAAACAAGTGAAGACATTGCAACATCTTCATCTCACAGTGAGTTTCCTTTAGCTGTTTCTTTGAACAGTGAAGACCTTAACAAGGTCTCTAGCAACATTTTATCAGCTGAAACTTGGCTTAGGTTAAACATTTTCCCATATTACCCTTCATCAAAAATCACCAACATTGTTGGAAAAACTTCAACTTTCTGCCAACAAGACCAAAACAACCTTAATGTTGTTCTACTTCTGTCTTCTTTGAAAAATCTCTATCATTCCCTCAAAAGATGGGGTTTAGAACAAGACATCAAAGTTTCCATTGCTTTTGATTTGGACTGTTTTACCCTTAACTCAGCTACTTCCAACCATGATTTGAAAATTTAA

Protein sequence

>MsG0580024748.01.T02

MSSHLESMKKLGFFNHNNIINIATIIPKERKITTRKLSVTTIPIKPTPSPEIAQPPLDFPAGSPYPAPYNVPNSKPLPPLAQIVSSPPPISSPYFAPQEQPSPLPPQFVSPANSPQNGFTCPPCNAIENGSPSPSHYPQIAPVQKLWCVAKPSVPDVTLQEALDYACGEGGADCLEITTPQGIVTILIHWLLMLLMLSIVTGKSIRELVELVTLVSFTVGLFSARFKCMKSCSCGFYY*

>MsG0580024748.01.T01

MKMVWLFHLLFLCSVCIAVSGQQNIEFLNLCETSEDIATSSSHSEFPLAVSLNSEDLNKVSSNILSAETWLRLNIFPYYPSSKITNIVGKTSTFCQQDQNNLNVVLLLSSLKNLYHSLKRWGLEQDIKVSIAFDLDCFTLNSATSNHDLKI*