Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002482.01 | MsG0480021194.01 | 0.813986 | 1.938713e-51 | 1.284953e-48 |
| MsG0280011388.01 | MsG0480021194.01 | 0.810396 | 1.177227e-50 | 7.087878e-48 |
| MsG0380013178.01 | MsG0480021194.01 | 0.801042 | 1.085167e-48 | 5.131188e-46 |
| MsG0480020136.01 | MsG0480021194.01 | 0.811794 | 5.859081e-51 | 3.661013e-48 |
| MsG0480020874.01 | MsG0480021194.01 | 0.829053 | 6.381960e-55 | 6.457662e-52 |
| MsG0480021194.01 | MsG0480022222.01 | 0.802876 | 4.555711e-49 | 2.256664e-46 |
| MsG0480021194.01 | MsG0480022340.01 | -0.809243 | 2.083872e-50 | 1.217189e-47 |
| MsG0480021194.01 | MsG0480022768.01 | 0.803999 | 2.665587e-49 | 1.359034e-46 |
| MsG0480021194.01 | MsG0480022795.01 | 0.814636 | 1.392718e-51 | 9.395289e-49 |
| MsG0480021194.01 | MsG0480023814.01 | 0.817124 | 3.880573e-52 | 2.801748e-49 |
| MsG0480021194.01 | MsG0680031333.01 | -0.802658 | 5.053017e-49 | 2.489088e-46 |
| MsG0480021194.01 | MsG0680035704.01 | 0.814956 | 1.183186e-51 | 8.050522e-49 |
| MsG0480021194.01 | MsG0780041137.01 | 0.800408 | 1.461664e-48 | 6.800844e-46 |
| MsG0480021194.01 | MsG0880043905.01 | 0.830396 | 3.007649e-55 | 3.164878e-52 |
| MsG0480021194.01 | MsG0880047448.01 | 0.800070 | 1.712834e-48 | 7.901198e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTTTGATAATTCCAAATTT+TGG | 0.177386 | 4:+54968550 | None:intergenic |
| TGGAATTATCAAACTTCATT+TGG | 0.241574 | 4:-54968542 | MsG0480021194.01.T01:CDS |
| GATGCGGTGGTTGATTTCTT+TGG | 0.258076 | 4:+54968739 | None:intergenic |
| CCTGTGAAGCGTCCAAAATT+TGG | 0.272451 | 4:-54968562 | MsG0480021194.01.T01:CDS |
| GGAATTATCAAACTTCATTT+GGG | 0.304283 | 4:-54968541 | MsG0480021194.01.T01:CDS |
| CTCCAAGGCTGCTGCTGTGT+TGG | 0.359397 | 4:-54968662 | MsG0480021194.01.T01:CDS |
| ATCATCTGCCACCTCGATCT+TGG | 0.365573 | 4:+54968693 | None:intergenic |
| CTCCAACACAGCAGCAGCCT+TGG | 0.406523 | 4:+54968660 | None:intergenic |
| TTCTCATTCTCATCGTCCTC+TGG | 0.438046 | 4:-54968623 | MsG0480021194.01.T01:CDS |
| TTGGAAGAAGTTATAACAGC+AGG | 0.519988 | 4:+54968712 | None:intergenic |
| TGATTTCTTTGGATTTGACA+TGG | 0.571108 | 4:+54968750 | None:intergenic |
| AACAGCAGGTTGAGATGCGG+TGG | 0.585201 | 4:+54968726 | None:intergenic |
| TTCCACCTCTACTGATGCTG+CGG | 0.591522 | 4:-54968593 | MsG0480021194.01.T01:CDS |
| AGCCGCAGCATCAGTAGAGG+TGG | 0.594129 | 4:+54968591 | None:intergenic |
| AGATGATTTATCTTTCTCCA+AGG | 0.619561 | 4:-54968677 | MsG0480021194.01.T01:CDS |
| GAATTATCAAACTTCATTTG+GGG | 0.621507 | 4:-54968540 | MsG0480021194.01.T01:CDS |
| TATAACTTCTTCCAAGATCG+AGG | 0.626622 | 4:-54968704 | MsG0480021194.01.T01:CDS |
| AACTTCTTCCAAGATCGAGG+TGG | 0.680993 | 4:-54968701 | MsG0480021194.01.T01:CDS |
| ATTAGCCGCAGCATCAGTAG+AGG | 0.681201 | 4:+54968588 | None:intergenic |
| TATAACAGCAGGTTGAGATG+CGG | 0.689445 | 4:+54968723 | None:intergenic |
| GAGGTGGAACACTTAACCAG+AGG | 0.716047 | 4:+54968607 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTTTGATAATTCCAAATTT+TGG | + | Chr4:54968708-54968727 | None:intergenic | 20.0% |
| ! | GAATTATCAAACTTCATTTG+GGG | - | Chr4:54968715-54968734 | MsG0480021194.01.T01:CDS | 25.0% |
| ! | GGAATTATCAAACTTCATTT+GGG | - | Chr4:54968714-54968733 | MsG0480021194.01.T01:CDS | 25.0% |
| ! | TGGAATTATCAAACTTCATT+TGG | - | Chr4:54968713-54968732 | MsG0480021194.01.T01:CDS | 25.0% |
| AGATGATTTATCTTTCTCCA+AGG | - | Chr4:54968578-54968597 | MsG0480021194.01.T01:CDS | 30.0% | |
| TATAACTTCTTCCAAGATCG+AGG | - | Chr4:54968551-54968570 | MsG0480021194.01.T01:CDS | 35.0% | |
| TTGGAAGAAGTTATAACAGC+AGG | + | Chr4:54968546-54968565 | None:intergenic | 35.0% | |
| ! | TATAACAGCAGGTTGAGATG+CGG | + | Chr4:54968535-54968554 | None:intergenic | 40.0% |
| AACTTCTTCCAAGATCGAGG+TGG | - | Chr4:54968554-54968573 | MsG0480021194.01.T01:CDS | 45.0% | |
| TTCTCATTCTCATCGTCCTC+TGG | - | Chr4:54968632-54968651 | MsG0480021194.01.T01:CDS | 45.0% | |
| ! | CCAAATTTTGGACGCTTCAC+AGG | + | Chr4:54968696-54968715 | None:intergenic | 45.0% |
| ! | CCTGTGAAGCGTCCAAAATT+TGG | - | Chr4:54968693-54968712 | MsG0480021194.01.T01:CDS | 45.0% |
| ! | GATGCGGTGGTTGATTTCTT+TGG | + | Chr4:54968519-54968538 | None:intergenic | 45.0% |
| ATCATCTGCCACCTCGATCT+TGG | + | Chr4:54968565-54968584 | None:intergenic | 50.0% | |
| ATTAGCCGCAGCATCAGTAG+AGG | + | Chr4:54968670-54968689 | None:intergenic | 50.0% | |
| GAGGTGGAACACTTAACCAG+AGG | + | Chr4:54968651-54968670 | None:intergenic | 50.0% | |
| TTCCACCTCTACTGATGCTG+CGG | - | Chr4:54968662-54968681 | MsG0480021194.01.T01:CDS | 50.0% | |
| ! | AACAGCAGGTTGAGATGCGG+TGG | + | Chr4:54968532-54968551 | None:intergenic | 55.0% |
| AGCCGCAGCATCAGTAGAGG+TGG | + | Chr4:54968667-54968686 | None:intergenic | 60.0% | |
| CTCCAACACAGCAGCAGCCT+TGG | + | Chr4:54968598-54968617 | None:intergenic | 60.0% | |
| CTCCAAGGCTGCTGCTGTGT+TGG | - | Chr4:54968593-54968612 | MsG0480021194.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 54968507 | 54968770 | 54968507 | ID=MsG0480021194.01;Name=MsG0480021194.01 |
| Chr4 | mRNA | 54968507 | 54968770 | 54968507 | ID=MsG0480021194.01.T01;Parent=MsG0480021194.01;Name=MsG0480021194.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|87 |
| Chr4 | exon | 54968507 | 54968770 | 54968507 | ID=MsG0480021194.01.T01:exon:6657;Parent=MsG0480021194.01.T01 |
| Chr4 | CDS | 54968507 | 54968770 | 54968507 | ID=MsG0480021194.01.T01:cds;Parent=MsG0480021194.01.T01 |
| Gene Sequence |
| Protein sequence |
