AlfalfaGEDB | MsG0280011388.01

M. sativa cultivar ZhongmuNo.1 / MsG0280011388.01

KEGG: K13280
GO: GO:0006465 GO:0008233 GO:0016020
PFAM: PF00717

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011388.01.T01	XP_003597879.1	93.333	180	0	1	1	168	1	180	1.01E-113	330

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011388.01.T01	Q5RC30	52.273	176	70	3	6	168	17	191	2.59E-57	181

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011388.01.T01	Q2HUB9	93.333	180	0	1	1	168	1	180	4.84e-114	330

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047814.01	MsG0280011388.01	0.857894	1.195601e-62	2.996384e-59
MsG0080048152.01	MsG0280011388.01	0.804494	2.102646e-49	1.085665e-46
MsG0080048383.01	MsG0280011388.01	0.833279	5.841318e-56	6.694995e-53
MsG0080048462.01	MsG0280011388.01	0.830631	2.634540e-55	2.791375e-52
MsG0080048687.01	MsG0280011388.01	0.852674	3.954253e-61	8.325466e-58
MsG0080048784.01	MsG0280011388.01	0.808997	2.352194e-50	1.364817e-47
MsG0080048855.01	MsG0280011388.01	0.816648	4.961687e-52	3.536109e-49
MsG0080048856.01	MsG0280011388.01	0.869884	2.215573e-66	8.448597e-63
MsG0080048926.01	MsG0280011388.01	0.810430	1.157461e-50	6.975266e-48
MsG0080048971.01	MsG0280011388.01	0.838821	2.289902e-57	3.103069e-54

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PPI

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Gene1	Gene2	Type
MsG0280011388.01	MsG0480022954.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011388.01.T01	MTR_2g103570	93.333	180	0	1	1	168	1	180	1.23e-117	330

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280011388.01.T01	AT1G52600	85.556	180	14	1	1	168	1	180	3.12e-109	309
MsG0280011388.01.T01	AT3G15710	79.444	180	25	1	1	168	1	180	1.96e-98	281

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Find 54 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGGCATTGATGTGCATCAC+TGG	0.537420	2:-83377880	MsG0280011388.01.T01:CDS
AATTAATATTATGCTGTTAA+TGG	0.174068	2:+83375680	None:intergenic
ACAATCTCTCCTGCGCGAAT+TGG	0.370765	2:+83377585	None:intergenic
ACATGAAAGAGGAGATACTG+GGG	0.746258	2:-83376737	MsG0280011388.01.T01:CDS
ACATTATGGGTAGAGCTGTT+GGG	0.526062	2:-83376453	MsG0280011388.01.T01:intron
ACGGATCTGAAGAGATTTGA+TGG	0.414274	2:+83378718	None:intergenic
AGAAACGCAATGGGTTTCGT+TGG	0.517115	2:-83378755	MsG0280011388.01.T01:exon
AGCAAACAAACCTTGATGAT+AGG	0.465854	2:+83376085	None:intergenic
AGTAAGGATCCAATTCGCGC+AGG	0.635833	2:-83377594	MsG0280011388.01.T01:CDS
ATAACAACTATGGGGATGAC+AGG	0.548283	2:-83376516	MsG0280011388.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACAGCAAAATACAAGAGA+CGG	+	Chr2:83377960-83377979	None:intergenic	30.0%
ACATTGCTTGTATTTGTCAA+TGG	-	Chr2:83378349-83378368	MsG0280011388.01.T01:intron	30.0%
ATTCAAGAAAGAAACGCAAT+GGG	-	Chr2:83375742-83375761	MsG0280011388.01.T01:three_prime_UTR	30.0%
CAGCTAAATGATAACACAAT+AGG	+	Chr2:83378454-83378473	None:intergenic	30.0%
GAATGAGATCTGTGATATTA+AGG	-	Chr2:83378157-83378176	MsG0280011388.01.T01:intron	30.0%
GAATTAGCGTAGGATAAATT+AGG	+	Chr2:83378266-83378285	None:intergenic	30.0%
GATAAATTAGGATAAACCCT+TGG	+	Chr2:83378254-83378273	None:intergenic	30.0%
GCCTATGTAGATTATATGAA+CGG	-	Chr2:83376507-83376526	MsG0280011388.01.T01:CDS	30.0%
GTGAGGAATTCTAATGTTAA+GGG	-	Chr2:83376198-83376217	MsG0280011388.01.T01:intron	30.0%
TGGATAAGATTGATATTACC+TGG	-	Chr2:83377672-83377691	MsG0280011388.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	83375660	83378868	83375660	ID=MsG0280011388.01;Name=MsG0280011388.01
Chr2	mRNA	83375660	83378868	83375660	ID=MsG0280011388.01.T01;Parent=MsG0280011388.01;Name=MsG0280011388.01.T01;_AED=0.39;_eAED=0.39;_QI=100\|0.83\|1\|1\|1\|1\|7\|134\|168
Chr2	exon	83378690	83378868	83378690	ID=MsG0280011388.01.T01:exon:14796;Parent=MsG0280011388.01.T01
Chr2	exon	83377822	83377934	83377822	ID=MsG0280011388.01.T01:exon:14797;Parent=MsG0280011388.01.T01
Chr2	exon	83377569	83377640	83377569	ID=MsG0280011388.01.T01:exon:14798;Parent=MsG0280011388.01.T01
Chr2	exon	83376716	83376761	83376716	ID=MsG0280011388.01.T01:exon:14799;Parent=MsG0280011388.01.T01
Chr2	exon	83376454	83376541	83376454	ID=MsG0280011388.01.T01:exon:14800;Parent=MsG0280011388.01.T01
Chr2	exon	83376096	83376153	83376096	ID=MsG0280011388.01.T01:exon:14801;Parent=MsG0280011388.01.T01
Chr2	exon	83375660	83375844	83375660	ID=MsG0280011388.01.T01:exon:14802;Parent=MsG0280011388.01.T01
Chr2	five_prime_UTR	83378769	83378868	83378769	ID=MsG0280011388.01.T01:five_prime_utr;Parent=MsG0280011388.01.T01
Chr2	CDS	83378690	83378768	83378690	ID=MsG0280011388.01.T01:cds;Parent=MsG0280011388.01.T01
Chr2	CDS	83377822	83377934	83377822	ID=MsG0280011388.01.T01:cds;Parent=MsG0280011388.01.T01
Chr2	CDS	83377569	83377640	83377569	ID=MsG0280011388.01.T01:cds;Parent=MsG0280011388.01.T01
Chr2	CDS	83376716	83376761	83376716	ID=MsG0280011388.01.T01:cds;Parent=MsG0280011388.01.T01
Chr2	CDS	83376454	83376541	83376454	ID=MsG0280011388.01.T01:cds;Parent=MsG0280011388.01.T01
Chr2	CDS	83376096	83376153	83376096	ID=MsG0280011388.01.T01:cds;Parent=MsG0280011388.01.T01
Chr2	CDS	83375794	83375844	83375794	ID=MsG0280011388.01.T01:cds;Parent=MsG0280011388.01.T01
Chr2	three_prime_UTR	83375660	83375793	83375660	ID=MsG0280011388.01.T01:three_prime_utr;Parent=MsG0280011388.01.T01

Gene Sequence

>MsG0280011388.01.T01

ATGGGTTTCGTTGGTGACACTGTCGATTCCATCAAATCTCTTCAGATCCGTCAGGTCCTCACTCAGGCTGTTAGCCTTGGCATGATTGTTACGTCTGCACTGATAATATGGAAGGCATTGATGTGCATCACTGGCAGTGAATCTCCTGTTGTCGTTGTTCTTTCTGGAAGTATGGAACCGGGATTCAAACGTGGGGACATCTTGTTCTTACACATGAGTAAGGATCCAATTCGCGCAGGAGAGATTGTTGTATTTAATATAGATGTACATGAAAGAGGAGATACTGGGGAGGTTGATGTTCTCACAAAAGGAGATAACAACTATGGGGATGACAGGCTACTGTATGCTCATGGCCAGCTTTGGCTGCAAAGGCACCACATTATGGGTAGAGCTGTTGGGTTCTTACCTTATGTTGGCTGGGTGACAATTATCATGACTGAAAAACCTATCATCAAGTATATTCTCATCGGTGCTTTGGGGTTGCTCGTGATAACTTCAAAAGAGTAA

Protein sequence

>MsG0280011388.01.T01

MGFVGDTVDSIKSLQIRQVLTQAVSLGMIVTSALIIWKALMCITGSESPVVVVLSGSMEPGFKRGDILFLHMSKDPIRAGEIVVFNIDVHERGDTGEVDVLTKGDNNYGDDRLLYAHGQLWLQRHHIMGRAVGFLPYVGWVTIIMTEKPIIKYILIGALGLLVITSKE*