AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041137.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041137.01.T01 MTR_7g104930 60.000 100 1 1 1 100 1 61 3.00e-32 108
MsG0780041137.01.T01 MTR_6g066250 95.455 44 2 0 57 100 19 62 2.82e-23 86.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041137.01.T01 AT2G23930 55.000 100 6 1 1 100 1 61 2.44e-29 101
MsG0780041137.01.T01 AT3G11500 55.000 100 6 1 1 100 1 61 3.83e-29 100
MsG0780041137.01.T01 AT3G11500 55.000 100 6 1 1 100 1 61 3.83e-29 100
MsG0780041137.01.T01 AT2G23930 88.571 35 4 0 66 100 1 35 2.81e-16 67.8

Find 26 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATGGTAGCTTATATTATT+TGG 0.139068 7:+87032530 MsG0780041137.01.T01:CDS
CTTCGACCAATTTATGAATT+TGG 0.200064 7:+87032904 MsG0780041137.01.T01:CDS
TGTAATTTCTCTCGCTTATA+TGG 0.223525 7:+87032513 MsG0780041137.01.T01:CDS
TCTAACAATTCTCAGAAGTT+AGG 0.238637 7:+87031800 None:intergenic
CAACAACCAAATTCATAAAT+TGG 0.293346 7:-87032910 None:intergenic
AATGCTTACTTCTTCAAATC+TGG 0.328903 7:-87031911 None:intergenic
TTCTTCACCATGAGTAGGTC+AGG 0.358279 7:+87031881 MsG0780041137.01.T01:exon
AACGGAGAACGAAGATGATT+TGG 0.363843 7:-87031856 None:intergenic
TTCTCAGAAGTTAGGGTTTG+TGG 0.369417 7:+87031808 None:intergenic
CTAACAATTCTCAGAAGTTA+GGG 0.391326 7:+87031801 None:intergenic
GTGTGGCATGTCTTTGCTAC+TGG 0.465421 7:+87032477 MsG0780041137.01.T01:CDS
AATGCAAATCGCATGATTGT+TGG 0.502115 7:+87032869 MsG0780041137.01.T01:CDS
TACTCATGGTGAAGAACAAA+CGG 0.507774 7:-87031874 None:intergenic
ACTTGATAATGCATAGTATG+AGG 0.515286 7:-87032989 None:intergenic
TCATAAATTGGTCGAAGCCA+CGG 0.529169 7:-87032898 None:intergenic
GGAGAACGAAGATGATTTGG+AGG 0.530047 7:-87031853 None:intergenic
AACGAAGATGATTTGGAGGG+TGG 0.546349 7:-87031849 None:intergenic
GTTTGTTCTTCACCATGAGT+AGG 0.564681 7:+87031876 MsG0780041137.01.T01:exon
GACAATACTGTGGAGGTGAA+TGG 0.565835 7:+87032932 MsG0780041137.01.T01:CDS
TGGCTGCCCTGACCTACTCA+TGG 0.567702 7:-87031888 None:intergenic
GCTTACTTCTTCAAATCTGG+TGG 0.598528 7:-87031908 None:intergenic
TCTTCACCATGAGTAGGTCA+GGG 0.618968 7:+87031882 MsG0780041137.01.T01:exon
ATGATTGTTGGTACTCTCCG+TGG 0.628986 7:+87032881 MsG0780041137.01.T01:CDS
TTTGGTTGTTGACAATACTG+TGG 0.664176 7:+87032922 MsG0780041137.01.T01:CDS
GAGAACGAAGATGATTTGGA+GGG 0.675668 7:-87031852 None:intergenic
GGTTGTTGACAATACTGTGG+AGG 0.683546 7:+87032925 MsG0780041137.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACCTTTTTTTTTTTTTTAA+TGG - Chr7:87032183-87032202 None:intergenic 10.0%
!!! TAATTTTTTAACTGAATTTT+TGG + Chr7:87032247-87032266 MsG0780041137.01.T01:intron 10.0%
!! AATATTAGTAGTTTATGTAT+AGG + Chr7:87033015-87033034 MsG0780041137.01.T01:CDS 15.0%
!! TATTCATATAAATCTTTCTA+CGG + Chr7:87032668-87032687 MsG0780041137.01.T01:intron 15.0%
!! TGCCATTAAAAAAAAAAAAA+AGG + Chr7:87032178-87032197 MsG0780041137.01.T01:intron 15.0%
!! ATATGGTAGCTTATATTATT+TGG + Chr7:87032530-87032549 MsG0780041137.01.T01:CDS 20.0%
!! ATTCATATAAATCTTTCTAC+GGG + Chr7:87032669-87032688 MsG0780041137.01.T01:intron 20.0%
!! CAATTCATAAAGAATGAAAA+CGG - Chr7:87032617-87032636 None:intergenic 20.0%
!!! GTTTTCATTCTTTATGAATT+GGG + Chr7:87032616-87032635 MsG0780041137.01.T01:intron 20.0%
!!! TTTTCATTCTTTATGAATTG+GGG + Chr7:87032617-87032636 MsG0780041137.01.T01:intron 20.0%
! ATATGTATCTATGTACATGT+GGG + Chr7:87032740-87032759 MsG0780041137.01.T01:intron 25.0%
! CAACAACCAAATTCATAAAT+TGG - Chr7:87032913-87032932 None:intergenic 25.0%
! TTAACCACTTCTCTATTAAT+TGG - Chr7:87032389-87032408 None:intergenic 25.0%
! TTTCATTCTTTATGAATTGG+GGG + Chr7:87032618-87032637 MsG0780041137.01.T01:intron 25.0%
!! CGTTTTCATTCTTTATGAAT+TGG + Chr7:87032615-87032634 MsG0780041137.01.T01:intron 25.0%
!!! ATTTTTTTTCGATAGGTACA+TGG + Chr7:87032015-87032034 MsG0780041137.01.T01:intron 25.0%
!!! GATATGCATTTTTTTTCGAT+AGG + Chr7:87032008-87032027 MsG0780041137.01.T01:intron 25.0%
AATGCTTACTTCTTCAAATC+TGG - Chr7:87031914-87031933 None:intergenic 30.0%
ATTTGAATTTCTACTGCACT+TGG + Chr7:87031977-87031996 MsG0780041137.01.T01:intron 30.0%
CATGTACATAGATACATATC+AGG - Chr7:87032739-87032758 None:intergenic 30.0%
CTTCGACCAATTTATGAATT+TGG + Chr7:87032904-87032923 MsG0780041137.01.T01:CDS 30.0%
GATATGTATCTATGTACATG+TGG + Chr7:87032739-87032758 MsG0780041137.01.T01:intron 30.0%
GCAATGAGAAAAATGACATA+GGG + Chr7:87032954-87032973 MsG0780041137.01.T01:CDS 30.0%
GGGTTTAACATGTGTATAAA+TGG + Chr7:87032760-87032779 MsG0780041137.01.T01:intron 30.0%
TGATCCAATTAATAGAGAAG+TGG + Chr7:87032382-87032401 MsG0780041137.01.T01:intron 30.0%
TGTAATTTCTCTCGCTTATA+TGG + Chr7:87032513-87032532 MsG0780041137.01.T01:CDS 30.0%
!! ACTTGATAATGCATAGTATG+AGG - Chr7:87032992-87033011 None:intergenic 30.0%
!! GTTTTTGTAGTTTGTGAAGA+TGG + Chr7:87032200-87032219 MsG0780041137.01.T01:intron 30.0%
AATGCAAATCGCATGATTGT+TGG + Chr7:87032869-87032888 MsG0780041137.01.T01:CDS 35.0%
CATGTCTTATGATTTAGCTG+AGG + Chr7:87032806-87032825 MsG0780041137.01.T01:intron 35.0%
GAGGATTATACTGAAGTATG+TGG + Chr7:87032825-87032844 MsG0780041137.01.T01:intron 35.0%
GCATTATAAAGTGAAGTTCG+AGG + Chr7:87032552-87032571 MsG0780041137.01.T01:intron 35.0%
GGCAATGAGAAAAATGACAT+AGG + Chr7:87032953-87032972 MsG0780041137.01.T01:CDS 35.0%
TACTCATGGTGAAGAACAAA+CGG - Chr7:87031877-87031896 None:intergenic 35.0%
TGAGAAAAATGACATAGGGA+TGG + Chr7:87032958-87032977 MsG0780041137.01.T01:CDS 35.0%
TTCTAGGTACAAGATTGAGT+GGG + Chr7:87032298-87032317 MsG0780041137.01.T01:intron 35.0%
TTTCTAGGTACAAGATTGAG+TGG + Chr7:87032297-87032316 MsG0780041137.01.T01:intron 35.0%
TTTGGTTGTTGACAATACTG+TGG + Chr7:87032922-87032941 MsG0780041137.01.T01:CDS 35.0%
AACGGAGAACGAAGATGATT+TGG - Chr7:87031859-87031878 None:intergenic 40.0%
CACTATTGCTATGAGTTCAG+TGG + Chr7:87032274-87032293 MsG0780041137.01.T01:intron 40.0%
CATATCAGGAACAAACAGAC+AGG - Chr7:87032725-87032744 None:intergenic 40.0%
CTATGAGTTCAGTGGTTTCT+AGG + Chr7:87032282-87032301 MsG0780041137.01.T01:intron 40.0%
GAAAAATGACATAGGGATGG+TGG + Chr7:87032961-87032980 MsG0780041137.01.T01:CDS 40.0%
GCTTACTTCTTCAAATCTGG+TGG - Chr7:87031911-87031930 None:intergenic 40.0%
GGACAAACAGCTTCAAAGTA+AGG + Chr7:87032036-87032055 MsG0780041137.01.T01:intron 40.0%
GTTTGTTCTTCACCATGAGT+AGG + Chr7:87031876-87031895 MsG0780041137.01.T01:exon 40.0%
TCATAAATTGGTCGAAGCCA+CGG - Chr7:87032901-87032920 None:intergenic 40.0%
TGGATTGCAGAGAAAAAGTG+TGG + Chr7:87032460-87032479 MsG0780041137.01.T01:intron 40.0%
! CTGTATGTGAGAGCTTTTAC+TGG + Chr7:87032423-87032442 MsG0780041137.01.T01:intron 40.0%
! GAGAACGAAGATGATTTGGA+GGG - Chr7:87031855-87031874 None:intergenic 40.0%
ATGATTGTTGGTACTCTCCG+TGG + Chr7:87032881-87032900 MsG0780041137.01.T01:CDS 45.0%
GACAATACTGTGGAGGTGAA+TGG + Chr7:87032932-87032951 MsG0780041137.01.T01:CDS 45.0%
GGTTGTTGACAATACTGTGG+AGG + Chr7:87032925-87032944 MsG0780041137.01.T01:CDS 45.0%
TCTTCACCATGAGTAGGTCA+GGG + Chr7:87031882-87031901 MsG0780041137.01.T01:exon 45.0%
TTCTTCACCATGAGTAGGTC+AGG + Chr7:87031881-87031900 MsG0780041137.01.T01:exon 45.0%
! AACGAAGATGATTTGGAGGG+TGG - Chr7:87031852-87031871 None:intergenic 45.0%
! GGAGAACGAAGATGATTTGG+AGG - Chr7:87031856-87031875 None:intergenic 45.0%
! TACTGGCTGCGAACATTCTT+TGG + Chr7:87032440-87032459 MsG0780041137.01.T01:intron 45.0%
!! CTATATATATAAATATAAAA+AGG - Chr7:87032156-87032175 None:intergenic 5.0%
!!! TTTTTTTTTTAAATCAAATT+TGG + Chr7:87032107-87032126 MsG0780041137.01.T01:intron 5.0%
GTGTGGCATGTCTTTGCTAC+TGG + Chr7:87032477-87032496 MsG0780041137.01.T01:CDS 50.0%
TGGCTGCCCTGACCTACTCA+TGG - Chr7:87031891-87031910 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 87031814 87033036 87031814 ID=MsG0780041137.01;Name=MsG0780041137.01
Chr7 mRNA 87031814 87033036 87031814 ID=MsG0780041137.01.T01;Parent=MsG0780041137.01;Name=MsG0780041137.01.T01;_AED=0.36;_eAED=0.59;_QI=76|0.33|0.5|1|0|0|4|0|116
Chr7 exon 87031814 87031924 87031814 ID=MsG0780041137.01.T01:exon:52100;Parent=MsG0780041137.01.T01
Chr7 exon 87032030 87032052 87032030 ID=MsG0780041137.01.T01:exon:52101;Parent=MsG0780041137.01.T01
Chr7 exon 87032470 87032566 87032470 ID=MsG0780041137.01.T01:exon:52102;Parent=MsG0780041137.01.T01
Chr7 exon 87032841 87033036 87032841 ID=MsG0780041137.01.T01:exon:52103;Parent=MsG0780041137.01.T01
Chr7 five_prime_UTR 87031814 87031889 87031814 ID=MsG0780041137.01.T01:five_prime_utr;Parent=MsG0780041137.01.T01
Chr7 CDS 87031890 87031924 87031890 ID=MsG0780041137.01.T01:cds;Parent=MsG0780041137.01.T01
Chr7 CDS 87032030 87032052 87032030 ID=MsG0780041137.01.T01:cds;Parent=MsG0780041137.01.T01
Chr7 CDS 87032470 87032566 87032470 ID=MsG0780041137.01.T01:cds;Parent=MsG0780041137.01.T01
Chr7 CDS 87032841 87033036 87032841 ID=MsG0780041137.01.T01:cds;Parent=MsG0780041137.01.T01
Gene Sequence

>MsG0780041137.01.T01

ATGAGTAGGTCAGGGCAGCCACCAGATTTGAAGAAGTACATGGACAAACAGCTTCAAAAGAAAAAGTGTGGCATGTCTTTGCTACTGGTTGATACTTTGAATGTAATTTCTCTCGCTTATATGGTAGCTTATATTATTTGGCATTATAAAGTGAATATGTGGTATTATCTTGCAGTCAAGCTGAATGCAAATCGCATGATTGTTGGTACTCTCCGTGGCTTCGACCAATTTATGAATTTGGTTGTTGACAATACTGTGGAGGTGAATGGCAATGAGAAAAATGACATAGGGATGGTGGTAAGTCCTCATACTATGCATTATCAAGTTATAATATTAGTAGTTTATGTATAG

Protein sequence

>MsG0780041137.01.T01

MSRSGQPPDLKKYMDKQLQKKKCGMSLLLVDTLNVISLAYMVAYIIWHYKVNMWYYLAVKLNANRMIVGTLRGFDQFMNLVVDNTVEVNGNEKNDIGMVVSPHTMHYQVIILVVYV*