AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017135.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017135.01.T01 MTR_3g101630 85.217 115 17 0 1 115 1 115 8.59e-67 200
MsG0380017135.01.T01 MTR_3g101630 86.726 113 15 0 1 113 1 113 1.44e-66 201
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017135.01.T01 AT4G33350 53.333 120 39 3 1 103 1 120 2.11e-31 111
MsG0380017135.01.T01 AT4G33350 53.333 120 39 3 1 103 1 120 3.29e-31 112

Find 40 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCGAGTGTGAGATTGAATT+AGG 0.307685 3:-92477367 None:intergenic
GGAGGTTCCGGCGAGGGATT+TGG 0.319748 3:-92477432 None:intergenic
ACTCGACTCGAATTAACTAT+TGG 0.338401 3:+92477125 MsG0380017135.01.T01:five_prime_UTR
AGGAAGCTTCAGCGTCTTCT+TGG 0.380666 3:-92477541 None:intergenic
CAAGGTGGTTACAGTGTTGA+TGG 0.389724 3:-92477307 None:intergenic
TCGGATTCTGTCTTAATTGT+GGG 0.393939 3:-92477171 None:intergenic
CTCGGATTCTGTCTTAATTG+TGG 0.407460 3:-92477172 None:intergenic
GTTAAAGCGGCGGCGAAAGC+GGG 0.432701 3:-92477395 None:intergenic
GTGAGATTGAATTAGGGTTC+CGG 0.435865 3:-92477360 None:intergenic
CTCGACTCGAATTAACTATT+GGG 0.450926 3:+92477126 MsG0380017135.01.T01:five_prime_UTR
GACGGTGTAAACGGAGGTTC+CGG 0.452524 3:-92477444 None:intergenic
GCGAGTGTGAGATTGAATTA+GGG 0.455909 3:-92477366 None:intergenic
AGCGGCGGCGAAAGCGGGGT+TGG 0.456106 3:-92477390 None:intergenic
GAGGGATTTGGCGACGTTGT+TGG 0.462263 3:-92477420 None:intergenic
GGAACTACTGACGGTGTAAA+CGG 0.467319 3:-92477453 None:intergenic
CATCAGAACGAATTCATTGT+TGG 0.485434 3:-92477474 None:intergenic
ACGCTGAAGCTTCCTTGCTC+AGG 0.498165 3:+92477549 MsG0380017135.01.T01:CDS
GCTGAAGGTGCTAAGTCCAT+TGG 0.515881 3:+92477503 MsG0380017135.01.T01:CDS
TTCATTGTTGGAACTACTGA+CGG 0.538242 3:-92477462 None:intergenic
GTTTATCCACGATCTAGTAA+AGG 0.539568 3:+92477201 MsG0380017135.01.T01:five_prime_UTR
TCTAATCCTTTACTAGATCG+TGG 0.567177 3:-92477207 None:intergenic
GTAAACGGAGGTTCCGGCGA+GGG 0.577581 3:-92477438 None:intergenic
GCGAAAGCAAAGCAATCCAA+TGG 0.584246 3:-92477519 None:intergenic
TGAATTTCGAGGCAATGCTA+AGG 0.584914 3:+92477672 MsG0380017135.01.T01:CDS
TGTCTTCAAAACGAGTTGCA+AGG 0.588693 3:-92477325 None:intergenic
AGTTAAAGCGGCGGCGAAAG+CGG 0.589283 3:-92477396 None:intergenic
AGAGGAACGTTACCTGAGCA+AGG 0.598379 3:-92477561 None:intergenic
GGACTTAGCACCTTCAGCAT+CGG 0.600686 3:-92477498 None:intergenic
GACGTTGTTGGAAGTTAAAG+CGG 0.609660 3:-92477408 None:intergenic
TCGTGGATAAACTATAAGCT+CGG 0.613169 3:-92477190 None:intergenic
AACGTCGCCAAATCCCTCGC+CGG 0.619652 3:+92477425 MsG0380017135.01.T01:CDS
AACCGCGAAAATGATAGAAG+CGG 0.620119 3:-92477281 None:intergenic
TTAAAGCGGCGGCGAAAGCG+GGG 0.621674 3:-92477394 None:intergenic
AATAGTTAATTCGAGTCGAG+TGG 0.643283 3:-92477123 None:intergenic
AAATGGAGTCACAAGGACAG+TGG 0.653796 3:+92477255 MsG0380017135.01.T01:exon
TGTAAACGGAGGTTCCGGCG+AGG 0.668767 3:-92477439 None:intergenic
GTTCTGATGTCCGATGCTGA+AGG 0.674395 3:+92477488 MsG0380017135.01.T01:CDS
GTTGTTGGAAGTTAAAGCGG+CGG 0.684073 3:-92477405 None:intergenic
CTTCAAAACGAGTTGCAAGG+TGG 0.698132 3:-92477322 None:intergenic
ACTACTGACGGTGTAAACGG+AGG 0.722610 3:-92477450 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAATTACTGAGAG+AGG - Chr3:92477582-92477601 None:intergenic 20.0%
!! ATTTGTAGAAGAAACAAAAA+TGG + Chr3:92477238-92477257 MsG0380017135.01.T01:exon 20.0%
!! AATGTTTTGCTCGTTGTTTT+AGG + Chr3:92477633-92477652 MsG0380017135.01.T01:intron 30.0%
AATAGTTAATTCGAGTCGAG+TGG - Chr3:92477126-92477145 None:intergenic 35.0%
CATCAGAACGAATTCATTGT+TGG - Chr3:92477477-92477496 None:intergenic 35.0%
GAAACAAAAATGGAGTCACA+AGG + Chr3:92477248-92477267 MsG0380017135.01.T01:exon 35.0%
GTTTATCCACGATCTAGTAA+AGG + Chr3:92477201-92477220 MsG0380017135.01.T01:five_prime_UTR 35.0%
TCACGGAAAAATGAATTTCG+AGG + Chr3:92477661-92477680 MsG0380017135.01.T01:CDS 35.0%
TCGGATTCTGTCTTAATTGT+GGG - Chr3:92477174-92477193 None:intergenic 35.0%
TCGTGGATAAACTATAAGCT+CGG - Chr3:92477193-92477212 None:intergenic 35.0%
TCTAATCCTTTACTAGATCG+TGG - Chr3:92477210-92477229 None:intergenic 35.0%
TTCATTGTTGGAACTACTGA+CGG - Chr3:92477465-92477484 None:intergenic 35.0%
! ACTCGACTCGAATTAACTAT+TGG + Chr3:92477125-92477144 MsG0380017135.01.T01:five_prime_UTR 35.0%
! CTCGACTCGAATTAACTATT+GGG + Chr3:92477126-92477145 MsG0380017135.01.T01:five_prime_UTR 35.0%
AACCGCGAAAATGATAGAAG+CGG - Chr3:92477284-92477303 None:intergenic 40.0%
CTCGGATTCTGTCTTAATTG+TGG - Chr3:92477175-92477194 None:intergenic 40.0%
GAAGACACAAAAAAACTCGC+CGG + Chr3:92477341-92477360 MsG0380017135.01.T01:CDS 40.0%
GACGTTGTTGGAAGTTAAAG+CGG - Chr3:92477411-92477430 None:intergenic 40.0%
GCGAGTGTGAGATTGAATTA+GGG - Chr3:92477369-92477388 None:intergenic 40.0%
GTGAGATTGAATTAGGGTTC+CGG - Chr3:92477363-92477382 None:intergenic 40.0%
TGAATTTCGAGGCAATGCTA+AGG + Chr3:92477672-92477691 MsG0380017135.01.T01:CDS 40.0%
TGCGAGTGTGAGATTGAATT+AGG - Chr3:92477370-92477389 None:intergenic 40.0%
TGTCTTCAAAACGAGTTGCA+AGG - Chr3:92477328-92477347 None:intergenic 40.0%
! ATCCGCTTCTATCATTTTCG+CGG + Chr3:92477279-92477298 MsG0380017135.01.T01:CDS 40.0%
!!! CGTTGTTTTAGGTTCGATCA+CGG + Chr3:92477644-92477663 MsG0380017135.01.T01:intron 40.0%
AAATGGAGTCACAAGGACAG+TGG + Chr3:92477255-92477274 MsG0380017135.01.T01:exon 45.0%
CTTCAAAACGAGTTGCAAGG+TGG - Chr3:92477325-92477344 None:intergenic 45.0%
GCGAAAGCAAAGCAATCCAA+TGG - Chr3:92477522-92477541 None:intergenic 45.0%
GGAACTACTGACGGTGTAAA+CGG - Chr3:92477456-92477475 None:intergenic 45.0%
GTTGTTGGAAGTTAAAGCGG+CGG - Chr3:92477408-92477427 None:intergenic 45.0%
! CAAGGTGGTTACAGTGTTGA+TGG - Chr3:92477310-92477329 None:intergenic 45.0%
ACTACTGACGGTGTAAACGG+AGG - Chr3:92477453-92477472 None:intergenic 50.0%
AGAGGAACGTTACCTGAGCA+AGG - Chr3:92477564-92477583 None:intergenic 50.0%
AGGAAGCTTCAGCGTCTTCT+TGG - Chr3:92477544-92477563 None:intergenic 50.0%
GGACTTAGCACCTTCAGCAT+CGG - Chr3:92477501-92477520 None:intergenic 50.0%
GTTCTGATGTCCGATGCTGA+AGG + Chr3:92477488-92477507 MsG0380017135.01.T01:CDS 50.0%
!! GCTGAAGGTGCTAAGTCCAT+TGG + Chr3:92477503-92477522 MsG0380017135.01.T01:CDS 50.0%
ACGCTGAAGCTTCCTTGCTC+AGG + Chr3:92477549-92477568 MsG0380017135.01.T01:CDS 55.0%
AGTTAAAGCGGCGGCGAAAG+CGG - Chr3:92477399-92477418 None:intergenic 55.0%
GACGGTGTAAACGGAGGTTC+CGG - Chr3:92477447-92477466 None:intergenic 55.0%
GAGGGATTTGGCGACGTTGT+TGG - Chr3:92477423-92477442 None:intergenic 55.0%
AACGTCGCCAAATCCCTCGC+CGG + Chr3:92477425-92477444 MsG0380017135.01.T01:CDS 60.0%
GTAAACGGAGGTTCCGGCGA+GGG - Chr3:92477441-92477460 None:intergenic 60.0%
GTTAAAGCGGCGGCGAAAGC+GGG - Chr3:92477398-92477417 None:intergenic 60.0%
TGTAAACGGAGGTTCCGGCG+AGG - Chr3:92477442-92477461 None:intergenic 60.0%
TTAAAGCGGCGGCGAAAGCG+GGG - Chr3:92477397-92477416 None:intergenic 60.0%
GGAGGTTCCGGCGAGGGATT+TGG - Chr3:92477435-92477454 None:intergenic 65.0%
AGCGGCGGCGAAAGCGGGGT+TGG - Chr3:92477393-92477412 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr3 gene 92477121 92477706 92477121 ID=MsG0380017135.01;Name=MsG0380017135.01
Chr3 mRNA 92477121 92477706 92477121 ID=MsG0380017135.01.T01;Parent=MsG0380017135.01;Name=MsG0380017135.01.T01;_AED=0.35;_eAED=0.35;_QI=136|1|1|1|0.12|0.11|9|0|121
Chr3 exon 92477121 92477570 92477121 ID=MsG0380017135.01.T01:exon:12576;Parent=MsG0380017135.01.T01
Chr3 exon 92477655 92477706 92477655 ID=MsG0380017135.01.T01:exon:12577;Parent=MsG0380017135.01.T01
Chr3 five_prime_UTR 92477121 92477256 92477121 ID=MsG0380017135.01.T01:five_prime_utr;Parent=MsG0380017135.01.T01
Chr3 CDS 92477257 92477570 92477257 ID=MsG0380017135.01.T01:cds;Parent=MsG0380017135.01.T01
Chr3 CDS 92477655 92477706 92477655 ID=MsG0380017135.01.T01:cds;Parent=MsG0380017135.01.T01
Gene Sequence

>MsG0380017135.01.T01

ATGGAGTCACAAGGACAGTGGAATCCGCTTCTATCATTTTCGCGGTTCATCCATCAACACTGTAACCACCTTGCAACTCGTTTTGAAGACACAAAAAAACTCGCCGGAACCCTAATTCAATCTCACACTCGCACCAACCCCGCTTTCGCCGCCGCTTTAACTTCCAACAACGTCGCCAAATCCCTCGCCGGAACCTCCGTTTACACCGTCAGTAGTTCCAACAATGAATTCGTTCTGATGTCCGATGCTGAAGGTGCTAAGTCCATTGGATTGCTTTGCTTTCGCCAAGAAGACGCTGAAGCTTCCTTGCTCAGGTTCGATCACGGAAAAATGAATTTCGAGGCAATGCTAAGGTTGTTCCAATAA

Protein sequence

>MsG0380017135.01.T01

MESQGQWNPLLSFSRFIHQHCNHLATRFEDTKKLAGTLIQSHTRTNPAFAAALTSNNVAKSLAGTSVYTVSSSNNEFVLMSDAEGAKSIGLLCFRQEDAEASLLRFDHGKMNFEAMLRLFQ*