AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680035777.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035777.01.T01 MTR_6g088745 96.682 211 6 1 1 211 1 210 8.18e-147 407
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035777.01.T01 AT2G37410 61.290 248 54 3 1 211 1 243 2.24e-98 286
MsG0680035777.01.T01 AT2G37410 61.290 248 54 3 1 211 1 243 2.24e-98 286
MsG0680035777.01.T01 AT1G20350 58.257 218 84 2 1 211 1 218 6.09e-85 251
MsG0680035777.01.T01 AT5G11690 58.268 127 53 0 1 127 1 127 2.60e-47 152

Find 73 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGTGGTAGTTTCGCTGTTT+GGG 0.213111 6:+112117373 MsG0680035777.01.T01:CDS
GGAGAATTCGAATACGAATT+TGG 0.234336 6:+112117652 MsG0680035777.01.T01:CDS
CAAGGCTCGCGTGAGGTTTC+CGG 0.263330 6:-112117197 None:intergenic
CTTGCTATGCGTCAGGGTTT+AGG 0.280627 6:+112117494 MsG0680035777.01.T01:CDS
CCGGTGAAACGTGCTCCTTT+AGG 0.286831 6:-112117314 None:intergenic
CGGTGAAACGTGCTCCTTTA+GGG 0.297343 6:-112117313 None:intergenic
AAGCCGTGGTTTGGTGGATT+TGG 0.323797 6:+112117692 MsG0680035777.01.T01:CDS
TCGGTGGTAGTTTCGCTGTT+TGG 0.324015 6:+112117372 MsG0680035777.01.T01:CDS
TCGGCGCGGTCGGCGGCGTT+TGG 0.332942 6:+112117521 MsG0680035777.01.T01:CDS
ATTGTTGCTGGTGCTGCTAC+TGG 0.376461 6:+112117464 MsG0680035777.01.T01:CDS
TGGTAGTGAGGTGAAGGTTT+TGG 0.385516 6:+112117757 MsG0680035777.01.T01:CDS
GACGACATCGGCGGTGCGTT+TGG 0.387738 6:+112117236 MsG0680035777.01.T01:CDS
CATCGGCGGTGCGTTTGGTA+TGG 0.397202 6:+112117241 MsG0680035777.01.T01:CDS
TGTAGAGACCTTTGAGGAAG+TGG 0.408598 6:-112117286 None:intergenic
CGACTCAACGCGCCTCGAAT+CGG 0.422021 6:+112117353 MsG0680035777.01.T01:CDS
CCGTGGAATTCTATTGTTGC+TGG 0.424117 6:+112117452 MsG0680035777.01.T01:CDS
CCGTGGTTTGGTGGATTTGG+TGG 0.430833 6:+112117695 MsG0680035777.01.T01:CDS
GCGCGGTCGGCGGCGTTTGG+TGG 0.436907 6:+112117524 MsG0680035777.01.T01:CDS
TTCGAATTCTCCTGCGCCCC+CGG 0.438450 6:-112117641 None:intergenic
CAGAAGGAGGAAGAGAAGAT+TGG 0.449106 6:+112117728 MsG0680035777.01.T01:CDS
GCGTCAGGGTTTAGGTGCTT+CGG 0.463311 6:+112117502 MsG0680035777.01.T01:CDS
TGGTGGTGGTAGTGAGGTGA+AGG 0.479849 6:+112117751 MsG0680035777.01.T01:CDS
TTTAGGTGCTTCGGCGCGGT+CGG 0.483924 6:+112117511 MsG0680035777.01.T01:CDS
AAAGGTGGGAAGCCGTGGTT+TGG 0.489022 6:+112117683 MsG0680035777.01.T01:CDS
GGTGGGAAGCCGTGGTTTGG+TGG 0.493009 6:+112117686 MsG0680035777.01.T01:CDS
AATCACTTATACTCAAAGTT+CGG 0.494929 6:-112117803 None:intergenic
AAGGAGGAAGAGAAGATTGG+TGG 0.495445 6:+112117731 MsG0680035777.01.T01:CDS
TCCGCTTTCCACTTCCTCAA+AGG 0.500336 6:+112117278 MsG0680035777.01.T01:CDS
GGTCTCTACAATTCCCCTAA+AGG 0.507760 6:+112117299 MsG0680035777.01.T01:CDS
GCCTTTGCCGATGATGATTG+AGG 0.510168 6:+112117610 MsG0680035777.01.T01:CDS
ATCGGCGGTGCGTTTGGTAT+GGG 0.511722 6:+112117242 MsG0680035777.01.T01:CDS
GAAGAGAAGATTGGTGGTGG+TGG 0.514173 6:+112117737 MsG0680035777.01.T01:CDS
CCTAAAGGAGCACGTTTCAC+CGG 0.521130 6:+112117314 MsG0680035777.01.T01:CDS
GAGGAAGAGAAGATTGGTGG+TGG 0.528373 6:+112117734 MsG0680035777.01.T01:CDS
ATGATTGAGGATATGCCACC+GGG 0.529612 6:+112117623 MsG0680035777.01.T01:CDS
TATGGTTTATGTGAGACAAA+AGG 0.530843 6:+112117424 MsG0680035777.01.T01:CDS
AGGTGCTTCGGCGCGGTCGG+CGG 0.538109 6:+112117514 MsG0680035777.01.T01:CDS
GGGAATTGTAGAGACCTTTG+AGG 0.542928 6:-112117292 None:intergenic
GATGTCGTCGAGGATGCGAT+CGG 0.550261 6:-112117222 None:intergenic
ATGTCGTCGAGGATGCGATC+GGG 0.552232 6:-112117221 None:intergenic
TACTCAAAGTTCGGCACAGG+AGG 0.552301 6:-112117794 None:intergenic
GCGAAACTACCACCGATTCG+AGG 0.553488 6:-112117365 None:intergenic
TCGAGGATGCGATCGGGACA+AGG 0.558352 6:-112117215 None:intergenic
TCCTCAATCATCATCGGCAA+AGG 0.567838 6:-112117611 None:intergenic
GATCGCATCCTCGACGACAT+CGG 0.568551 6:+112117224 MsG0680035777.01.T01:CDS
GTTGCTGGTGCTGCTACTGG+TGG 0.571703 6:+112117467 MsG0680035777.01.T01:CDS
GATGATTGAGGATATGCCAC+CGG 0.573026 6:+112117622 MsG0680035777.01.T01:CDS
GGTAGTTTCGCTGTTTGGGG+TGG 0.580485 6:+112117377 MsG0680035777.01.T01:CDS
GGTGGTAGTTTCGCTGTTTG+GGG 0.580757 6:+112117374 MsG0680035777.01.T01:CDS
TTTGGTATGGGAGCCGTCGG+TGG 0.584337 6:+112117254 MsG0680035777.01.T01:CDS
GGCATATCCTCAATCATCAT+CGG 0.585759 6:-112117617 None:intergenic
CTCAACGCGCCTCGAATCGG+TGG 0.594285 6:+112117356 MsG0680035777.01.T01:CDS
GTGGAAAGCGGAGCCACCGA+CGG 0.594868 6:-112117267 None:intergenic
TTATACTCAAAGTTCGGCAC+AGG 0.596938 6:-112117797 None:intergenic
GAATTCGAATACGAATTTGG+AGG 0.597122 6:+112117655 MsG0680035777.01.T01:CDS
GCGTTTGGTATGGGAGCCGT+CGG 0.599391 6:+112117251 MsG0680035777.01.T01:CDS
TCGAACAGCTTGTGTTGCGC+CGG 0.607726 6:-112117333 None:intergenic
ACCTTTGAGGAAGTGGAAAG+CGG 0.610627 6:-112117279 None:intergenic
AAAACAAAGGTGGGAAGCCG+TGG 0.612739 6:+112117678 MsG0680035777.01.T01:CDS
GATTGGTGGTGGTGGTAGTG+AGG 0.613196 6:+112117745 MsG0680035777.01.T01:CDS
ATCGGGACAAGGCTCGCGTG+AGG 0.616293 6:-112117204 None:intergenic
CGCATCCTCGACGACATCGG+CGG 0.629489 6:+112117227 MsG0680035777.01.T01:CDS
ATTTGGAGGAAAACAAAGGT+GGG 0.635580 6:+112117669 MsG0680035777.01.T01:CDS
AATTTGGAGGAAAACAAAGG+TGG 0.642320 6:+112117668 MsG0680035777.01.T01:CDS
TGAGACAAAAGGAAGATCCG+TGG 0.646748 6:+112117435 MsG0680035777.01.T01:CDS
ACGAATTTGGAGGAAAACAA+AGG 0.650310 6:+112117665 MsG0680035777.01.T01:CDS
GGTGAAACGTGCTCCTTTAG+GGG 0.651062 6:-112117312 None:intergenic
GATTGAGGATATGCCACCGG+GGG 0.657615 6:+112117625 MsG0680035777.01.T01:CDS
CCACCAAATCCACCAAACCA+CGG 0.665947 6:-112117695 None:intergenic
ACGCACCGCCGATGTCGTCG+AGG 0.669526 6:-112117232 None:intergenic
AGGGTTTAGGTGCTTCGGCG+CGG 0.674148 6:+112117507 MsG0680035777.01.T01:CDS
CCAGCAACAATAGAATTCCA+CGG 0.674217 6:-112117452 None:intergenic
TGATTGAGGATATGCCACCG+GGG 0.779991 6:+112117624 MsG0680035777.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCAACTTTTGATTGTACTA+TGG + Chr6:112117406-112117425 MsG0680035777.01.T01:CDS 25.0%
TATGGTTTATGTGAGACAAA+AGG + Chr6:112117424-112117443 MsG0680035777.01.T01:CDS 30.0%
AATTTGGAGGAAAACAAAGG+TGG + Chr6:112117668-112117687 MsG0680035777.01.T01:CDS 35.0%
ACGAATTTGGAGGAAAACAA+AGG + Chr6:112117665-112117684 MsG0680035777.01.T01:CDS 35.0%
ATTTGGAGGAAAACAAAGGT+GGG + Chr6:112117669-112117688 MsG0680035777.01.T01:CDS 35.0%
GAATTCGAATACGAATTTGG+AGG + Chr6:112117655-112117674 MsG0680035777.01.T01:CDS 35.0%
GGAGAATTCGAATACGAATT+TGG + Chr6:112117652-112117671 MsG0680035777.01.T01:CDS 35.0%
CCAGCAACAATAGAATTCCA+CGG - Chr6:112117455-112117474 None:intergenic 40.0%
GGCATATCCTCAATCATCAT+CGG - Chr6:112117620-112117639 None:intergenic 40.0%
TTATACTCAAAGTTCGGCAC+AGG - Chr6:112117800-112117819 None:intergenic 40.0%
!! TGGAATTTTTGGACAGAAGG+AGG + Chr6:112117715-112117734 MsG0680035777.01.T01:CDS 40.0%
!! TGGTGGAATTTTTGGACAGA+AGG + Chr6:112117712-112117731 MsG0680035777.01.T01:CDS 40.0%
!!! GGTGGATTTGGTGGAATTTT+TGG + Chr6:112117704-112117723 MsG0680035777.01.T01:CDS 40.0%
!!! GTGCTTTTAGCGTTGATTGA+GGG + Chr6:112117548-112117567 MsG0680035777.01.T01:CDS 40.0%
!!! TGCTTTTAGCGTTGATTGAG+GGG + Chr6:112117549-112117568 MsG0680035777.01.T01:CDS 40.0%
!!! TGTGCTTTTAGCGTTGATTG+AGG + Chr6:112117547-112117566 MsG0680035777.01.T01:CDS 40.0%
AAGGAGGAAGAGAAGATTGG+TGG + Chr6:112117731-112117750 MsG0680035777.01.T01:CDS 45.0%
ATGATTGAGGATATGCCACC+GGG + Chr6:112117623-112117642 MsG0680035777.01.T01:CDS 45.0%
CAGAAGGAGGAAGAGAAGAT+TGG + Chr6:112117728-112117747 MsG0680035777.01.T01:CDS 45.0%
CCGTGGAATTCTATTGTTGC+TGG + Chr6:112117452-112117471 MsG0680035777.01.T01:CDS 45.0%
GATGATTGAGGATATGCCAC+CGG + Chr6:112117622-112117641 MsG0680035777.01.T01:CDS 45.0%
GGGAATTGTAGAGACCTTTG+AGG - Chr6:112117295-112117314 None:intergenic 45.0%
GGTCTCTACAATTCCCCTAA+AGG + Chr6:112117299-112117318 MsG0680035777.01.T01:CDS 45.0%
TCCTCAATCATCATCGGCAA+AGG - Chr6:112117614-112117633 None:intergenic 45.0%
TGAGACAAAAGGAAGATCCG+TGG + Chr6:112117435-112117454 MsG0680035777.01.T01:CDS 45.0%
! ACCTTTGAGGAAGTGGAAAG+CGG - Chr6:112117282-112117301 None:intergenic 45.0%
! TGTAGAGACCTTTGAGGAAG+TGG - Chr6:112117289-112117308 None:intergenic 45.0%
!! GGTTTTCTTGCTATGCGTCA+GGG + Chr6:112117488-112117507 MsG0680035777.01.T01:CDS 45.0%
!! TGGTAGTGAGGTGAAGGTTT+TGG + Chr6:112117757-112117776 MsG0680035777.01.T01:CDS 45.0%
!! TGGTTTTCTTGCTATGCGTC+AGG + Chr6:112117487-112117506 MsG0680035777.01.T01:CDS 45.0%
!!! GCTTTTAGCGTTGATTGAGG+GGG + Chr6:112117550-112117569 MsG0680035777.01.T01:CDS 45.0%
AAAACAAAGGTGGGAAGCCG+TGG + Chr6:112117678-112117697 MsG0680035777.01.T01:CDS 50.0%
CCACCAAATCCACCAAACCA+CGG - Chr6:112117698-112117717 None:intergenic 50.0%
CGGTGAAACGTGCTCCTTTA+GGG - Chr6:112117316-112117335 None:intergenic 50.0%
CGGTGGTAGTTTCGCTGTTT+GGG + Chr6:112117373-112117392 MsG0680035777.01.T01:CDS 50.0%
GAGGAAGAGAAGATTGGTGG+TGG + Chr6:112117734-112117753 MsG0680035777.01.T01:CDS 50.0%
GCCTTTGCCGATGATGATTG+AGG + Chr6:112117610-112117629 MsG0680035777.01.T01:CDS 50.0%
GGTGAAACGTGCTCCTTTAG+GGG - Chr6:112117315-112117334 None:intergenic 50.0%
GGTGGTAGTTTCGCTGTTTG+GGG + Chr6:112117374-112117393 MsG0680035777.01.T01:CDS 50.0%
TACTCAAAGTTCGGCACAGG+AGG - Chr6:112117797-112117816 None:intergenic 50.0%
TCCGCTTTCCACTTCCTCAA+AGG + Chr6:112117278-112117297 MsG0680035777.01.T01:CDS 50.0%
TCGGTGGTAGTTTCGCTGTT+TGG + Chr6:112117372-112117391 MsG0680035777.01.T01:CDS 50.0%
TGATTGAGGATATGCCACCG+GGG + Chr6:112117624-112117643 MsG0680035777.01.T01:CDS 50.0%
! CCTAAAGGAGCACGTTTCAC+CGG + Chr6:112117314-112117333 MsG0680035777.01.T01:CDS 50.0%
! CTTGCTATGCGTCAGGGTTT+AGG + Chr6:112117494-112117513 MsG0680035777.01.T01:CDS 50.0%
!! AAGCCGTGGTTTGGTGGATT+TGG + Chr6:112117692-112117711 MsG0680035777.01.T01:CDS 50.0%
!! ATTGTTGCTGGTGCTGCTAC+TGG + Chr6:112117464-112117483 MsG0680035777.01.T01:CDS 50.0%
!! GAAGAGAAGATTGGTGGTGG+TGG + Chr6:112117737-112117756 MsG0680035777.01.T01:CDS 50.0%
AAAGGTGGGAAGCCGTGGTT+TGG + Chr6:112117683-112117702 MsG0680035777.01.T01:CDS 55.0%
ATCGGCGGTGCGTTTGGTAT+GGG + Chr6:112117242-112117261 MsG0680035777.01.T01:CDS 55.0%
ATGTCGTCGAGGATGCGATC+GGG - Chr6:112117224-112117243 None:intergenic 55.0%
CCGGTGAAACGTGCTCCTTT+AGG - Chr6:112117317-112117336 None:intergenic 55.0%
GATCGCATCCTCGACGACAT+CGG + Chr6:112117224-112117243 MsG0680035777.01.T01:CDS 55.0%
GATGTCGTCGAGGATGCGAT+CGG - Chr6:112117225-112117244 None:intergenic 55.0%
GATTGAGGATATGCCACCGG+GGG + Chr6:112117625-112117644 MsG0680035777.01.T01:CDS 55.0%
GCGTCAGGGTTTAGGTGCTT+CGG + Chr6:112117502-112117521 MsG0680035777.01.T01:CDS 55.0%
GGTAGTTTCGCTGTTTGGGG+TGG + Chr6:112117377-112117396 MsG0680035777.01.T01:CDS 55.0%
TCGAACAGCTTGTGTTGCGC+CGG - Chr6:112117336-112117355 None:intergenic 55.0%
!! CCGTGGTTTGGTGGATTTGG+TGG + Chr6:112117695-112117714 MsG0680035777.01.T01:CDS 55.0%
!! GATTGGTGGTGGTGGTAGTG+AGG + Chr6:112117745-112117764 MsG0680035777.01.T01:CDS 55.0%
!! GCGAAACTACCACCGATTCG+AGG - Chr6:112117368-112117387 None:intergenic 55.0%
!! TGGTGGTGGTAGTGAGGTGA+AGG + Chr6:112117751-112117770 MsG0680035777.01.T01:CDS 55.0%
!! TTAGCGTTGATTGAGGGGGC+TGG + Chr6:112117554-112117573 MsG0680035777.01.T01:CDS 55.0%
AGGGTTTAGGTGCTTCGGCG+CGG + Chr6:112117507-112117526 MsG0680035777.01.T01:CDS 60.0%
CAAGGCTCGCGTGAGGTTTC+CGG - Chr6:112117200-112117219 None:intergenic 60.0%
CATCGGCGGTGCGTTTGGTA+TGG + Chr6:112117241-112117260 MsG0680035777.01.T01:CDS 60.0%
CGACTCAACGCGCCTCGAAT+CGG + Chr6:112117353-112117372 MsG0680035777.01.T01:CDS 60.0%
GCGTTTGGTATGGGAGCCGT+CGG + Chr6:112117251-112117270 MsG0680035777.01.T01:CDS 60.0%
TCGAGGATGCGATCGGGACA+AGG - Chr6:112117218-112117237 None:intergenic 60.0%
TTCGAATTCTCCTGCGCCCC+CGG - Chr6:112117644-112117663 None:intergenic 60.0%
TTTAGGTGCTTCGGCGCGGT+CGG + Chr6:112117511-112117530 MsG0680035777.01.T01:CDS 60.0%
TTTGGTATGGGAGCCGTCGG+TGG + Chr6:112117254-112117273 MsG0680035777.01.T01:CDS 60.0%
!! GTTGCTGGTGCTGCTACTGG+TGG + Chr6:112117467-112117486 MsG0680035777.01.T01:CDS 60.0%
ATCGGGACAAGGCTCGCGTG+AGG - Chr6:112117207-112117226 None:intergenic 65.0%
CGCATCCTCGACGACATCGG+CGG + Chr6:112117227-112117246 MsG0680035777.01.T01:CDS 65.0%
CTCAACGCGCCTCGAATCGG+TGG + Chr6:112117356-112117375 MsG0680035777.01.T01:CDS 65.0%
GACGACATCGGCGGTGCGTT+TGG + Chr6:112117236-112117255 MsG0680035777.01.T01:CDS 65.0%
GTGGAAAGCGGAGCCACCGA+CGG - Chr6:112117270-112117289 None:intergenic 65.0%
! GGTGGGAAGCCGTGGTTTGG+TGG + Chr6:112117686-112117705 MsG0680035777.01.T01:CDS 65.0%
ACGCACCGCCGATGTCGTCG+AGG - Chr6:112117235-112117254 None:intergenic 70.0%
GAATTCTCCTGCGCCCCCGG+TGG - Chr6:112117641-112117660 None:intergenic 70.0%
AGGTGCTTCGGCGCGGTCGG+CGG + Chr6:112117514-112117533 MsG0680035777.01.T01:CDS 75.0%
! GGATATGCCACCGGGGGCGC+AGG + Chr6:112117631-112117650 MsG0680035777.01.T01:CDS 75.0%
! GCGCGGTCGGCGGCGTTTGG+TGG + Chr6:112117524-112117543 MsG0680035777.01.T01:CDS 80.0%
! TCGGCGCGGTCGGCGGCGTT+TGG + Chr6:112117521-112117540 MsG0680035777.01.T01:CDS 80.0%
Chromosome Type Strat End Strand Name
Chr6 gene 112117188 112117823 112117188 ID=MsG0680035777.01;Name=MsG0680035777.01
Chr6 mRNA 112117188 112117823 112117188 ID=MsG0680035777.01.T01;Parent=MsG0680035777.01;Name=MsG0680035777.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|211
Chr6 exon 112117188 112117823 112117188 ID=MsG0680035777.01.T01:exon:15028;Parent=MsG0680035777.01.T01
Chr6 CDS 112117188 112117823 112117188 ID=MsG0680035777.01.T01:cds;Parent=MsG0680035777.01.T01
Gene Sequence

>MsG0680035777.01.T01

ATGGGAACACCGGAAACCTCACGCGAGCCTTGTCCCGATCGCATCCTCGACGACATCGGCGGTGCGTTTGGTATGGGAGCCGTCGGTGGCTCCGCTTTCCACTTCCTCAAAGGTCTCTACAATTCCCCTAAAGGAGCACGTTTCACCGGCGCAACACAAGCTGTTCGACTCAACGCGCCTCGAATCGGTGGTAGTTTCGCTGTTTGGGGTGGTTTGTTTTCAACTTTTGATTGTACTATGGTTTATGTGAGACAAAAGGAAGATCCGTGGAATTCTATTGTTGCTGGTGCTGCTACTGGTGGTTTTCTTGCTATGCGTCAGGGTTTAGGTGCTTCGGCGCGGTCGGCGGCGTTTGGTGGTGTGCTTTTAGCGTTGATTGAGGGGGCTGGAATTGCTTTGAATAAGTTTTTGAGTGCTCAGCAGCCTTTGCCGATGATGATTGAGGATATGCCACCGGGGGCGCAGGAGAATTCGAATACGAATTTGGAGGAAAACAAAGGTGGGAAGCCGTGGTTTGGTGGATTTGGTGGAATTTTTGGACAGAAGGAGGAAGAGAAGATTGGTGGTGGTGGTAGTGAGGTGAAGGTTTTGGAGAGTTTTGATGCGCCTCCTGTGCCGAACTTTGAGTATAAGTGA

Protein sequence

>MsG0680035777.01.T01

MGTPETSREPCPDRILDDIGGAFGMGAVGGSAFHFLKGLYNSPKGARFTGATQAVRLNAPRIGGSFAVWGGLFSTFDCTMVYVRQKEDPWNSIVAGAATGGFLAMRQGLGASARSAAFGGVLLALIEGAGIALNKFLSAQQPLPMMIEDMPPGAQENSNTNLEENKGGKPWFGGFGGIFGQKEEEKIGGGGSEVKVLESFDAPPVPNFEYK*