AlfalfaGEDB | MsG0480021914.01

M. sativa cultivar ZhongmuNo.1 / MsG0480021914.01

KEGG: K03028
GO: GO:0005488
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021914.01.T01	XP_003607505.1	99.452	365	2	0	1	365	522	886	0	745
MsG0480021914.01.T02	KEH30710.1	99.065	107	1	0	19	125	415	521	7.27E-66	223

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021914.01.T01	Q9SIV2	88.219	365	43	0	1	365	527	891	0	655
MsG0480021914.01.T02	Q9SIV2	80.374	107	21	0	19	125	420	526	5.40E-54	184

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021914.01.T01	G7JTX3	99.452	365	2	0	1	365	522	886	0.0	745
MsG0480021914.01.T02	A0A072UXV8	99.065	107	1	0	19	125	415	521	3.47e-66	223

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047814.01	MsG0480021914.01	0.828184	1.035410e-54	1.021577e-51
MsG0080048058.01	MsG0480021914.01	0.853923	1.733071e-61	3.805475e-58
MsG0080048602.01	MsG0480021914.01	0.821836	3.266840e-53	2.687874e-50
MsG0080048765.01	MsG0480021914.01	0.819001	1.460507e-52	1.110486e-49
MsG0180000001.01	MsG0480021914.01	0.851266	9.926322e-61	1.998024e-57
MsG0180000101.01	MsG0480021914.01	0.884741	1.480100e-71	9.881396e-68
MsG0180000146.01	MsG0480021914.01	0.810357	1.199841e-50	7.216967e-48
MsG0180000235.01	MsG0480021914.01	0.860622	1.816464e-63	4.996570e-60
MsG0180000300.01	MsG0480021914.01	0.821588	3.728263e-53	3.045706e-50
MsG0180000401.01	MsG0480021914.01	0.886173	4.306529e-72	3.043123e-68

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021914.01.T01	MTR_4g078780	99.452	365	2	1	365	522	886	0.0	745
MsG0480021914.01.T01	MTR_4g078780	99.452	365	2	1	365	521	885	0.0	744
MsG0480021914.01.T01	MTR_4g078780	99.593	246	1	1	246	522	767	6.15e-174	503
MsG0480021914.01.T01	MTR_1g058950	83.333	96	16	263	358	4	99	7.92e-51	165
MsG0480021914.01.T01	MTR_0265s0020	87.719	57	7	302	358	145	201	3.06e-27	107
MsG0480021914.01.T02	MTR_4g078780	99.065	107	1	19	125	415	521	8.81e-70	223
MsG0480021914.01.T02	MTR_4g078780	99.065	107	1	19	125	415	521	3.47e-69	223
MsG0480021914.01.T02	MTR_4g078780	99.065	107	1	19	125	414	520	3.73e-69	223

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021914.01.T01	AT2G20580	88.219	365	43	1	365	527	891	0.0	655
MsG0480021914.01.T01	AT4G28470	88.219	365	43	1	365	527	891	0.0	654
MsG0480021914.01.T02	AT2G20580	80.374	107	21	19	125	420	526	5.51e-55	184
MsG0480021914.01.T02	AT4G28470	73.832	107	28	19	125	420	526	1.84e-49	168

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Find 110 sgRNAs with CRISPR-Local

Find 589 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAATTCCAAGTTAAGTGA+AGG	0.556109	4:-65908095	MsG0480021914.01.T01:five_prime_UTR
AAGAGCAACCTTCTGCAACC+CGG	0.510865	4:+65911460	None:intergenic
AAGTTTCTACGGCCACATTA+CGG	0.488041	4:-65911257	MsG0480021914.01.T01:five_prime_UTR
AATAGTTGCCTTTCACATGA+AGG	0.510708	4:-65910452	MsG0480021914.01.T01:intron
AATGATAACCATGTCATTGC+TGG	0.449019	4:-65906009	MsG0480021914.01.T01:five_prime_UTR
AATGCAATTTGCAGTGCATT+TGG	0.325383	4:+65908659	None:intergenic
AATGTCTGCTGAGGGAGAAC+GGG	0.467324	4:-65911059	MsG0480021914.01.T01:intron
ACCAGATGATATGCTAGTAT+TGG	0.515944	4:-65908720	MsG0480021914.01.T01:five_prime_UTR
ACTAGCATATCATCTGGTCC+AGG	0.575933	4:+65908725	None:intergenic
ACTGCAAATTGCATTGTTCA+TGG	0.433433	4:-65908651	MsG0480021914.01.T01:five_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATAAGCAGAAACAGACA+GGG	-	Chr4:65907802-65907821	MsG0480021914.01.T01:intron	30.0%
AAAGGACAATCCAGATTATA+GGG	-	Chr4:65911628-65911647	MsG0480021914.01.T01:intron	30.0%
AACAATTCCAAGTTAAGTGA+AGG	-	Chr4:65907116-65907135	MsG0480021914.01.T01:intron	30.0%
AACTAAATACTGTTCAGACA+CGG	+	Chr4:65911692-65911711	None:intergenic	30.0%
AACTTCAATAATAGATAGGC+CGG	-	Chr4:65905549-65905568	MsG0480021914.01.T01:CDS	30.0%
AAGACCAAAAAAGGATAGAT+AGG	-	Chr4:65907486-65907505	MsG0480021914.01.T01:intron	30.0%
AATCATTGTTGTCTATGGTT+TGG	-	Chr4:65910649-65910668	MsG0480021914.01.T01:intron	30.0%
AATTGGGGTGAATAGTTTAA+AGG	-	Chr4:65906313-65906332	MsG0480021914.01.T01:intron	30.0%
ACACAATAGCTTTCATATCA+AGG	+	Chr4:65911178-65911197	None:intergenic	30.0%
ACATTGACCTGATCAAATAA+AGG	+	Chr4:65910556-65910575	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	65903359	65911874	65903359	ID=MsG0480021914.01;Name=MsG0480021914.01
Chr4	mRNA	65903359	65911874	65903359	ID=MsG0480021914.01.T01;Parent=MsG0480021914.01;Name=MsG0480021914.01.T01;_AED=0.43;_eAED=0.43;_QI=1678\|1\|1\|1\|0.91\|0.88\|25\|216\|365
Chr4	exon	65905954	65906093	65905954	ID=MsG0480021914.01.T01:exon:18199;Parent=MsG0480021914.01.T01
Chr4	exon	65906186	65906290	65906186	ID=MsG0480021914.01.T01:exon:18198;Parent=MsG0480021914.01.T01
Chr4	exon	65906387	65906494	65906387	ID=MsG0480021914.01.T01:exon:18197;Parent=MsG0480021914.01.T01
Chr4	exon	65908031	65908189	65908031	ID=MsG0480021914.01.T01:exon:18196;Parent=MsG0480021914.01.T01
Chr4	exon	65908423	65908497	65908423	ID=MsG0480021914.01.T01:exon:18195;Parent=MsG0480021914.01.T01
Chr4	exon	65908640	65908754	65908640	ID=MsG0480021914.01.T01:exon:18194;Parent=MsG0480021914.01.T01
Chr4	exon	65910129	65910211	65910129	ID=MsG0480021914.01.T01:exon:18193;Parent=MsG0480021914.01.T01
Chr4	exon	65910301	65910339	65910301	ID=MsG0480021914.01.T01:exon:18192;Parent=MsG0480021914.01.T01
Chr4	exon	65910453	65910518	65910453	ID=MsG0480021914.01.T01:exon:18191;Parent=MsG0480021914.01.T01
Chr4	exon	65910652	65910694	65910652	ID=MsG0480021914.01.T01:exon:18190;Parent=MsG0480021914.01.T01
Chr4	exon	65910869	65910936	65910869	ID=MsG0480021914.01.T01:exon:18189;Parent=MsG0480021914.01.T01
Chr4	exon	65911060	65911119	65911060	ID=MsG0480021914.01.T01:exon:18188;Parent=MsG0480021914.01.T01
Chr4	exon	65911211	65911331	65911211	ID=MsG0480021914.01.T01:exon:18187;Parent=MsG0480021914.01.T01
Chr4	exon	65911449	65911549	65911449	ID=MsG0480021914.01.T01:exon:18186;Parent=MsG0480021914.01.T01
Chr4	exon	65911659	65911874	65911659	ID=MsG0480021914.01.T01:exon:18185;Parent=MsG0480021914.01.T01
Chr4	five_prime_UTR	65911659	65911874	65911659	ID=MsG0480021914.01.T01:five_prime_utr;Parent=MsG0480021914.01.T01
Chr4	five_prime_UTR	65911449	65911549	65911449	ID=MsG0480021914.01.T01:five_prime_utr;Parent=MsG0480021914.01.T01
Chr4	five_prime_UTR	65911211	65911331	65911211	ID=MsG0480021914.01.T01:five_prime_utr;Parent=MsG0480021914.01.T01
Chr4	five_prime_UTR	65911060	65911119	65911060	ID=MsG0480021914.01.T01:five_prime_utr;Parent=MsG0480021914.01.T01
Chr4	five_prime_UTR	65910869	65910936	65910869	ID=MsG0480021914.01.T01:five_prime_utr;Parent=MsG0480021914.01.T01
Chr4	five_prime_UTR	65910652	65910694	65910652	ID=MsG0480021914.01.T01:five_prime_utr;Parent=MsG0480021914.01.T01
Chr4	five_prime_UTR	65910453	65910518	65910453	ID=MsG0480021914.01.T01:five_prime_utr;Parent=MsG0480021914.01.T01
Chr4	five_prime_UTR	65910301	65910339	65910301	ID=MsG0480021914.01.T01:five_prime_utr;Parent=MsG0480021914.01.T01
Chr4	five_prime_UTR	65910129	65910211	65910129	ID=MsG0480021914.01.T01:five_prime_utr;Parent=MsG0480021914.01.T01
Chr4	five_prime_UTR	65908640	65908754	65908640	ID=MsG0480021914.01.T01:five_prime_utr;Parent=MsG0480021914.01.T01
Chr4	five_prime_UTR	65908423	65908497	65908423	ID=MsG0480021914.01.T01:five_prime_utr;Parent=MsG0480021914.01.T01
Chr4	five_prime_UTR	65908031	65908189	65908031	ID=MsG0480021914.01.T01:five_prime_utr;Parent=MsG0480021914.01.T01
Chr4	five_prime_UTR	65906387	65906494	65906387	ID=MsG0480021914.01.T01:five_prime_utr;Parent=MsG0480021914.01.T01
Chr4	five_prime_UTR	65906186	65906290	65906186	ID=MsG0480021914.01.T01:five_prime_utr;Parent=MsG0480021914.01.T01
Chr4	five_prime_UTR	65905954	65906093	65905954	ID=MsG0480021914.01.T01:five_prime_utr;Parent=MsG0480021914.01.T01
Chr4	five_prime_UTR	65905764	65905868	65905764	ID=MsG0480021914.01.T01:five_prime_utr;Parent=MsG0480021914.01.T01
Chr4	five_prime_UTR	65905599	65905672	65905599	ID=MsG0480021914.01.T01:five_prime_utr;Parent=MsG0480021914.01.T01
Chr4	CDS	65905452	65905598	65905452	ID=MsG0480021914.01.T01:cds;Parent=MsG0480021914.01.T01
Chr4	CDS	65905127	65905240	65905127	ID=MsG0480021914.01.T01:cds;Parent=MsG0480021914.01.T01
Chr4	CDS	65904817	65905032	65904817	ID=MsG0480021914.01.T01:cds;Parent=MsG0480021914.01.T01
Chr4	CDS	65904606	65904665	65904606	ID=MsG0480021914.01.T01:cds;Parent=MsG0480021914.01.T01
Chr4	CDS	65904409	65904522	65904409	ID=MsG0480021914.01.T01:cds;Parent=MsG0480021914.01.T01
Chr4	CDS	65904244	65904329	65904244	ID=MsG0480021914.01.T01:cds;Parent=MsG0480021914.01.T01
Chr4	CDS	65903989	65904097	65903989	ID=MsG0480021914.01.T01:cds;Parent=MsG0480021914.01.T01
Chr4	CDS	65903731	65903909	65903731	ID=MsG0480021914.01.T01:cds;Parent=MsG0480021914.01.T01
Chr4	CDS	65903575	65903647	65903575	ID=MsG0480021914.01.T01:cds;Parent=MsG0480021914.01.T01
Chr4	three_prime_UTR	65903359	65903574	65903359	ID=MsG0480021914.01.T01:three_prime_utr;Parent=MsG0480021914.01.T01
Chr4	mRNA	65903359	65906148	65903359	ID=MsG0480021914.01.T02;Parent=MsG0480021914.01;Name=MsG0480021914.01.T02;_AED=0.46;_eAED=0.48;_QI=0\|1\|0.90\|1\|1\|0.90\|11\|1304\|127
Chr4	exon	65905954	65906148	65905954	ID=MsG0480021914.01.T02:exon:18210;Parent=MsG0480021914.01.T02
Chr4	CDS	65905954	65906148	65905954	ID=MsG0480021914.01.T02:cds;Parent=MsG0480021914.01.T02
Chr4	CDS	65905764	65905868	65905764	ID=MsG0480021914.01.T02:cds;Parent=MsG0480021914.01.T02
Chr4	CDS	65905589	65905672	65905589	ID=MsG0480021914.01.T02:cds;Parent=MsG0480021914.01.T02
Chr4	three_prime_UTR	65905452	65905588	65905452	ID=MsG0480021914.01.T02:three_prime_utr;Parent=MsG0480021914.01.T02
Chr4	three_prime_UTR	65905127	65905240	65905127	ID=MsG0480021914.01.T02:three_prime_utr;Parent=MsG0480021914.01.T02
Chr4	three_prime_UTR	65904817	65905032	65904817	ID=MsG0480021914.01.T02:three_prime_utr;Parent=MsG0480021914.01.T02
Chr4	three_prime_UTR	65904606	65904665	65904606	ID=MsG0480021914.01.T02:three_prime_utr;Parent=MsG0480021914.01.T02
Chr4	three_prime_UTR	65904409	65904522	65904409	ID=MsG0480021914.01.T02:three_prime_utr;Parent=MsG0480021914.01.T02
Chr4	three_prime_UTR	65904244	65904329	65904244	ID=MsG0480021914.01.T02:three_prime_utr;Parent=MsG0480021914.01.T02
Chr4	three_prime_UTR	65903989	65904097	65903989	ID=MsG0480021914.01.T02:three_prime_utr;Parent=MsG0480021914.01.T02
Chr4	three_prime_UTR	65903731	65903909	65903731	ID=MsG0480021914.01.T02:three_prime_utr;Parent=MsG0480021914.01.T02
Chr4	three_prime_UTR	65903359	65903647	65903359	ID=MsG0480021914.01.T02:three_prime_utr;Parent=MsG0480021914.01.T02

Gene Sequence

>MsG0480021914.01.T01

ATGGGCTTGATTTACGTGGGTTCTTGCAATGAGGAAGTTGCTCAAGCAATCATATTTACATTGATGGATCGGAGTGAGTCAGAGTTGGGAGAGCCTCTTACGCGACTTCTCCCACTTGGCCTTGGTCTTCTTTATCTTGGGAAGCAGGAAAGTGTTGAGGCAACTGCCGAAGTTTCAAAGACTTTTAATGAAAAAATAAGAAAGTATTGTGACATGACACTACTATCATGTGCCTATGCTGGAACTGGGAATGTTCTCAAGGTGCAAAATCTTTTGGGTCATTGTTCACAACATCTTGACAAAGGTGAGATGCACCAAGGTCCTGCAGTCCTTGGAATTGCTATGGTAGCAATGGCTGAAGAATTAGGTGTTGAGATGGCTATCCGATCGTTAGAACATCTTCTACAGTATGGAGAGCAGAATATTCGCCGAGCAGTTCCTCTGGCACTTGGTCTGCTGTGTATATCAAACCCAAAGGTCAATGTTATGGATACCTTAAGCAGACTTAGCCATGATACTGATTCAGAAGTTGCAATGGCAGCAGTTATCTCCTTGGGTCTAATAGGCGCTGGAACAAACAATGCTCGAATTGCCGGCATGCTTCGCAATCTTTCAAGCTATTACTACAAGGATACCAGCCTTCTATTTTGTGTGCGGATTGCCCAAGGTCTTGTACATTTGGGAAAGGGACTATTAACACTTAATCCTTATCATTCTGACCGTGTCTTACTATCACCGACAGCGCTTGCAGGACTAATTACTATGTTGCATGCATGCCTTGATATGAAAGCTATTGTGTTGGGAAAATATCACTATGTTCTCTACTTCCTCGTTTTGGCCATGCAGCCGAGGATGTTGTTGACTGTGGATGAGGACTTGAAGCCTCTATCAGTGCCTGTTCGGGTTGGCCAAGCAGTAGACGTTGTTGGACAGGCAGGTCGTCCTAAGACAATCACCGGCTTTCAGACTCATTCTACCCCTGTTCTCCTGGCTGCTGGGGATAGGGCAGAGCTAGCCACAGAAAAATATCTTCCCCTATCACCAATTCTTGAAGGTTTTGTAATTTTAAAGGACAATCCAGATTATAGGGAAGAGTGA

>MsG0480021914.01.T02

ATGGATCACATAAATTTCATTTTCTATACTTATGTTATCCCTTGTATTATAACAGGTATGATTTTACTATGGGATGTTGACTCTGGACTTGCACAACTTGACAAATACTTCCATAGTAATGATAACCATGTCATTGCTGGTGCATTACTGGGAGTTGGAATTGTCAATTGTAGTATCAAGAATGACTGTGATCCTGCAATGGCACTCCTTGGTGATTTTATTGAAAAAGAAGATAGTTCGACTAGAATTGGTGCAATTATGGGATTAGGAATTGCATATGCTGGTTCCCAGAATGAGCAGTTACGATACAAGTTGGCACCTATTCTGAGTGATCCTAAAGCTTCGCTTGACGTGATTGCCTTCACTGCAATTGCATGGGCTTGA

Protein sequence

>MsG0480021914.01.T01

MGLIYVGSCNEEVAQAIIFTLMDRSESELGEPLTRLLPLGLGLLYLGKQESVEATAEVSKTFNEKIRKYCDMTLLSCAYAGTGNVLKVQNLLGHCSQHLDKGEMHQGPAVLGIAMVAMAEELGVEMAIRSLEHLLQYGEQNIRRAVPLALGLLCISNPKVNVMDTLSRLSHDTDSEVAMAAVISLGLIGAGTNNARIAGMLRNLSSYYYKDTSLLFCVRIAQGLVHLGKGLLTLNPYHSDRVLLSPTALAGLITMLHACLDMKAIVLGKYHYVLYFLVLAMQPRMLLTVDEDLKPLSVPVRVGQAVDVVGQAGRPKTITGFQTHSTPVLLAAGDRAELATEKYLPLSPILEGFVILKDNPDYREE*

>MsG0480021914.01.T02

MDHINFIFYTYVIPCIITGMILLWDVDSGLAQLDKYFHSNDNHVIAGALLGVGIVNCSIKNDCDPAMALLGDFIEKEDSSTRIGAIMGLGIAYAGSQNEQLRYKLAPILSDPKASLDVIAFTAIAWA*