AlfalfaGEDB | MsG0480023138.01

M. sativa cultivar ZhongmuNo.1 / MsG0480023138.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023138.01.T01	MBA0736564.1	56.25	160	51	1	3	143	38	197	2.61E-51	172

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023138.01.T01	Q8L5Z1	54.255	94	24	1	3	77	36	129	3.50E-23	96.3

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023138.01.T01	A0A1U8P8R5	56.875	160	50	1	3	143	38	197	8.33e-52	175

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0380014221.01	MsG0480023138.01	0.854309	1.340934e-61	2.982080e-58
MsG0480023138.01	MsG0480023150.01	0.821964	3.051263e-53	2.519492e-50
MsG0480023138.01	MsG0580024952.01	0.864676	1.024105e-64	3.242687e-61
MsG0480023138.01	MsG0580025053.01	0.880859	3.879781e-70	2.222956e-66
MsG0480023138.01	MsG0580028407.01	0.830629	2.636896e-55	2.793702e-52
MsG0480023138.01	MsG0680031263.01	0.879889	8.620523e-70	4.755253e-66
MsG0480023138.01	MsG0680031264.01	0.841501	4.574110e-58	6.734348e-55
MsG0480023138.01	MsG0680031621.01	0.869112	3.951413e-66	1.465358e-62
MsG0480023138.01	MsG0680031702.01	0.819445	1.157324e-52	8.907763e-50
MsG0480023138.01	MsG0780039940.01	0.896477	3.552396e-76	3.849552e-72
MsG0480023138.01	MsG0780040430.01	0.808326	3.272328e-50	1.865655e-47
MsG0480023138.01	MsG0880041897.01	0.820273	7.483749e-53	5.894490e-50
MsG0480023138.01	MsG0880045162.01	0.863050	3.282655e-64	9.818035e-61
MsG0480023138.01	MsG0880045735.01	0.849682	2.764981e-60	5.283661e-57
MsG0480023138.01	MsG0880046421.01	0.870137	1.831015e-66	7.045240e-63
MsG0380017893.01	MsG0480023138.01	0.853330	2.566327e-61	5.525412e-58
MsG0280006592.01	MsG0480023138.01	0.825767	3.916699e-54	3.603777e-51
MsG0280008899.01	MsG0480023138.01	0.820610	6.264294e-53	4.979910e-50

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023138.01.T01	MTR_8g087870	85.714	77	10	1	63	138	190	266	4.65e-38	132
MsG0480023138.01.T01	MTR_8g087870	74.725	91	4	1	3	74	31	121	1.22e-37	131
MsG0480023138.01.T01	MTR_3g023220	64.368	87	13	1	3	71	35	121	1.70e-29	110
MsG0480023138.01.T01	MTR_3g023220	47.619	63	29	1	76	138	208	266	1.96e-12	63.5
MsG0480023138.01.T01	MTR_2g017925	65.517	87	11	1	3	70	35	121	1.70e-29	110
MsG0480023138.01.T01	MTR_2g017925	58.730	63	25	1	74	136	207	268	5.31e-17	76.6
MsG0480023138.01.T01	MTR_2g017955	65.517	87	11	1	3	70	35	121	2.60e-29	110
MsG0480023138.01.T01	MTR_2g017955	51.613	62	29	1	75	136	208	268	1.12e-14	70.1
MsG0480023138.01.T01	MTR_2g017915	63.218	87	13	1	3	70	35	121	1.11e-28	108
MsG0480023138.01.T01	MTR_2g017875	65.169	89	11	2	3	71	36	124	1.28e-27	105
MsG0480023138.01.T01	MTR_2g017875	47.619	63	32	1	76	138	212	273	1.14e-12	64.3
MsG0480023138.01.T01	MTR_3g005660	56.383	94	22	1	3	77	38	131	3.13e-27	104
MsG0480023138.01.T01	MTR_0565s0010	56.044	91	21	1	3	74	34	124	1.97e-25	99.4
MsG0480023138.01.T01	MTR_3g024510	57.143	91	20	1	3	74	40	130	7.62e-25	98.2
MsG0480023138.01.T01	MTR_3g024510	33.962	106	56	3	33	138	184	275	4.53e-13	65.5
MsG0480023138.01.T01	MTR_2g017965	59.091	88	16	2	3	70	35	122	1.82e-24	97.1
MsG0480023138.01.T01	MTR_2g017965	50.000	62	30	1	75	136	209	269	4.93e-13	65.5
MsG0480023138.01.T01	MTR_2g018015	56.522	92	20	1	3	74	38	129	6.27e-22	89.4
MsG0480023138.01.T01	MTR_8g087890	44.030	134	43	5	3	110	33	160	1.97e-20	85.9
MsG0480023138.01.T01	MTR_8g087890	41.096	73	43	0	66	138	196	268	5.69e-13	65.1
MsG0480023138.01.T01	MTR_3g005650	51.648	91	26	1	3	75	32	122	3.73e-20	85.1
MsG0480023138.01.T01	MTR_2g033930	49.438	89	24	2	3	71	34	121	1.16e-19	84.0
MsG0480023138.01.T01	MTR_3g098270	47.959	98	28	2	3	77	36	133	2.50e-19	83.2
MsG0480023138.01.T01	MTR_2g033920	52.222	90	22	2	2	71	36	124	5.88e-19	81.3
MsG0480023138.01.T01	MTR_2g033920	52.222	90	22	2	2	71	36	124	1.38e-18	80.9
MsG0480023138.01.T01	MTR_2g017925	58.730	63	25	1	74	136	104	165	3.40e-17	75.9
MsG0480023138.01.T01	MTR_2g461580	49.438	89	24	1	3	70	33	121	4.87e-17	76.6
MsG0480023138.01.T01	MTR_4g035353	43.956	91	31	1	3	73	33	123	2.36e-16	74.7
MsG0480023138.01.T01	MTR_3g021460	49.438	89	24	1	3	70	33	121	2.50e-16	74.7
MsG0480023138.01.T01	MTR_4g122200	50.562	89	23	2	3	71	37	124	5.03e-16	73.9
MsG0480023138.01.T01	MTR_1g079530	45.161	93	30	2	3	74	31	123	5.38e-16	73.6
MsG0480023138.01.T01	MTR_8g079050	47.872	94	25	2	2	71	38	131	3.18e-15	71.6
MsG0480023138.01.T01	MTR_5g078210	45.833	96	30	3	1	74	25	120	5.60e-15	70.5
MsG0480023138.01.T01	MTR_2g087292	31.214	173	76	5	5	135	37	208	7.61e-15	69.7
MsG0480023138.01.T01	MTR_7g116570	43.011	93	32	2	3	74	36	128	9.85e-15	70.1
MsG0480023138.01.T01	MTR_5g078210	45.833	96	30	3	1	74	25	120	1.00e-14	70.1
MsG0480023138.01.T01	MTR_1g079590	39.785	93	35	2	3	74	33	125	1.15e-13	65.9
MsG0480023138.01.T01	MTR_1g025700	42.353	85	27	2	3	65	52	136	2.39e-13	66.2
MsG0480023138.01.T01	MTR_7g116520	41.935	93	33	2	3	74	36	128	2.63e-13	66.2
MsG0480023138.01.T01	MTR_7g116510	42.708	96	33	3	3	77	27	121	3.19e-13	65.9
MsG0480023138.01.T01	MTR_1g069175	39.623	106	42	2	3	86	35	140	3.63e-13	65.9
MsG0480023138.01.T01	MTR_4g108690	43.299	97	32	3	1	74	27	123	6.41e-13	65.1
MsG0480023138.01.T01	MTR_1g024890	42.222	90	31	2	3	71	34	123	6.60e-13	65.1
MsG0480023138.01.T01	MTR_0009s0250	43.182	88	28	2	5	70	37	124	9.97e-13	64.3
MsG0480023138.01.T01	MTR_1g025720	42.353	85	27	2	3	65	39	123	1.43e-12	63.9
MsG0480023138.01.T01	MTR_1g079540	39.785	93	35	2	3	74	33	125	1.61e-12	63.9
MsG0480023138.01.T01	MTR_5g084750	41.837	98	35	2	5	80	34	131	1.82e-12	63.5
MsG0480023138.01.T01	MTR_1g025780	39.000	100	39	2	5	82	45	144	1.86e-12	63.5
MsG0480023138.01.T01	MTR_1g025680	38.000	100	36	4	5	78	38	137	1.93e-12	63.9
MsG0480023138.01.T01	MTR_5g009100	41.667	96	35	2	3	77	48	143	2.12e-12	63.5
MsG0480023138.01.T01	MTR_7g116500	40.860	93	34	2	3	74	35	127	2.59e-12	63.2
MsG0480023138.01.T01	MTR_3g082830	41.000	100	37	3	5	82	42	141	3.31e-12	63.2
MsG0480023138.01.T01	MTR_3g435540	41.284	109	35	4	1	86	23	125	3.78e-12	62.8
MsG0480023138.01.T01	MTR_6g021530	41.304	92	32	2	5	74	33	124	4.06e-12	62.8
MsG0480023138.01.T01	MTR_3g114120	37.755	98	39	2	5	80	51	148	4.77e-12	62.4
MsG0480023138.01.T01	MTR_6g049220	45.570	79	33	2	3	80	32	101	5.46e-12	60.5
MsG0480023138.01.T01	MTR_2g084585	40.659	91	32	2	3	71	32	122	7.87e-12	62.0
MsG0480023138.01.T01	MTR_5g084790	48.387	62	32	0	76	137	207	268	1.46e-11	61.2
MsG0480023138.01.T01	MTR_5g084790	37.000	100	41	2	5	82	36	135	7.40e-11	59.3
MsG0480023138.01.T01	MTR_5g022640	42.857	84	25	2	5	65	47	130	1.76e-11	60.8
MsG0480023138.01.T01	MTR_1g025760	36.275	102	43	2	3	82	82	183	2.13e-11	60.8
MsG0480023138.01.T01	MTR_5g084880	43.284	67	38	0	76	142	28	94	4.18e-11	57.8
MsG0480023138.01.T01	MTR_8g031420	42.045	88	31	2	3	70	37	124	6.68e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023138.01.T01	AT1G33811	54.255	94	24	1	3	77	36	129	3.57e-24	96.3
MsG0480023138.01.T01	AT1G33811	40.845	71	39	2	66	133	201	271	1.20e-11	61.6
MsG0480023138.01.T01	AT5G45670	54.945	91	22	1	3	74	31	121	1.34e-23	94.7
MsG0480023138.01.T01	AT4G18970	54.945	91	22	1	3	74	30	120	1.51e-23	94.4
MsG0480023138.01.T01	AT4G18970	54.945	91	22	1	3	74	30	120	1.81e-23	94.7
MsG0480023138.01.T01	AT2G19010	52.747	91	24	1	3	74	32	122	3.17e-23	93.6
MsG0480023138.01.T01	AT2G19010	52.747	91	24	1	3	74	62	152	4.04e-23	93.6
MsG0480023138.01.T01	AT1G29660	53.846	91	22	2	3	74	34	123	3.58e-22	90.9
MsG0480023138.01.T01	AT1G29670	53.846	91	22	2	3	74	34	123	2.97e-21	88.2
MsG0480023138.01.T01	AT2G19050	52.747	91	24	1	3	74	33	123	3.74e-21	87.0
MsG0480023138.01.T01	AT1G29670	53.846	91	22	2	3	74	102	191	3.75e-21	88.6
MsG0480023138.01.T01	AT2G19050	52.747	91	24	1	3	74	33	123	6.62e-21	87.0
MsG0480023138.01.T01	AT2G19060	50.549	91	26	1	3	74	32	122	2.69e-19	82.8
MsG0480023138.01.T01	AT5G15720	52.747	91	22	2	3	73	32	121	5.02e-19	81.6
MsG0480023138.01.T01	AT1G71691	52.874	87	23	1	3	71	57	143	5.07e-19	81.6
MsG0480023138.01.T01	AT4G16230	53.333	90	20	2	3	71	32	120	5.57e-19	80.5
MsG0480023138.01.T01	AT1G71691	52.874	87	23	1	3	71	57	143	7.30e-19	82.0
MsG0480023138.01.T01	AT5G15720	52.747	91	22	2	3	73	32	121	9.00e-19	81.6
MsG0480023138.01.T01	AT5G08460	50.538	93	25	2	3	74	51	143	9.23e-19	81.6
MsG0480023138.01.T01	AT5G08460	39.130	69	42	0	66	134	216	284	3.42e-11	60.1
MsG0480023138.01.T01	AT4G30140	49.451	91	27	1	3	74	35	125	1.59e-18	80.5
MsG0480023138.01.T01	AT2G23540	47.872	94	25	2	2	71	49	142	5.12e-16	73.9
MsG0480023138.01.T01	AT1G71250	46.067	89	28	1	3	71	43	131	9.19e-16	73.2
MsG0480023138.01.T01	AT4G28780	44.340	106	36	4	3	86	33	137	1.42e-15	72.4
MsG0480023138.01.T01	AT5G41890	47.312	93	25	2	3	71	33	125	2.57e-15	72.0
MsG0480023138.01.T01	AT3G50400	46.316	95	27	2	2	72	35	129	6.99e-15	70.5
MsG0480023138.01.T01	AT4G16220	48.352	91	25	2	3	72	32	121	2.53e-14	68.2
MsG0480023138.01.T01	AT5G18430	42.553	94	32	2	3	74	30	123	8.47e-14	67.4
MsG0480023138.01.T01	AT1G20130	42.353	85	27	2	3	65	206	290	1.61e-13	67.0
MsG0480023138.01.T01	AT1G74460	45.872	109	30	4	1	86	23	125	1.67e-13	66.6
MsG0480023138.01.T01	AT1G20130	42.353	85	27	2	3	65	186	270	1.71e-13	67.0
MsG0480023138.01.T01	AT1G20132	41.111	90	31	2	3	70	63	152	2.35e-13	66.2
MsG0480023138.01.T01	AT5G37690	42.708	96	33	3	1	74	27	122	2.67e-13	66.2
MsG0480023138.01.T01	AT3G04290	41.489	94	34	2	3	75	31	124	2.77e-13	66.2
MsG0480023138.01.T01	AT5G33370	41.758	91	32	2	5	74	34	124	2.79e-13	65.9
MsG0480023138.01.T01	AT5G33370	41.758	91	32	2	5	74	34	124	4.61e-13	65.5
MsG0480023138.01.T01	AT3G14820	39.362	94	35	2	5	76	36	129	6.30e-13	65.1
MsG0480023138.01.T01	AT2G03980	42.857	91	30	2	6	74	48	138	1.71e-12	63.9
MsG0480023138.01.T01	AT2G03980	42.857	91	30	2	6	74	48	138	1.71e-12	63.9
MsG0480023138.01.T01	AT2G03980	42.857	91	30	2	6	74	48	138	1.71e-12	63.9
MsG0480023138.01.T01	AT2G03980	42.857	91	30	2	6	74	48	138	1.71e-12	63.9
MsG0480023138.01.T01	AT4G10950	44.944	89	32	2	3	74	73	161	1.91e-12	63.9
MsG0480023138.01.T01	AT2G04570	44.444	81	43	1	59	139	188	266	2.13e-12	63.5
MsG0480023138.01.T01	AT2G04020	44.318	88	27	2	6	71	48	135	2.20e-12	62.8
MsG0480023138.01.T01	AT2G04020	44.318	88	27	2	6	71	48	135	3.09e-12	63.2
MsG0480023138.01.T01	AT1G75890	38.384	99	35	4	5	77	51	149	4.27e-12	62.8
MsG0480023138.01.T01	AT3G43570	37.234	94	37	2	5	76	34	127	4.32e-12	62.4
MsG0480023138.01.T01	AT5G42170	38.144	97	38	2	5	79	54	150	4.41e-12	62.8
MsG0480023138.01.T01	AT3G43550	37.234	94	37	2	5	76	34	127	4.41e-12	62.4
MsG0480023138.01.T01	AT1G59406	37.234	94	37	2	5	76	34	127	5.03e-12	62.4
MsG0480023138.01.T01	AT1G59030	37.234	94	37	2	5	76	34	127	5.03e-12	62.4
MsG0480023138.01.T01	AT1G58725	37.234	94	37	2	5	76	34	127	5.03e-12	62.4
MsG0480023138.01.T01	AT4G10950	43.011	93	32	2	3	74	73	165	6.43e-12	62.4
MsG0480023138.01.T01	AT1G75880	38.144	97	37	3	5	79	56	151	8.13e-12	62.0
MsG0480023138.01.T01	AT1G75890	41.667	84	26	3	5	65	51	134	1.45e-11	61.2
MsG0480023138.01.T01	AT1G75890	41.667	84	26	3	5	65	80	163	1.45e-11	61.2
MsG0480023138.01.T01	AT1G75880	39.759	83	28	2	5	65	56	138	3.03e-11	60.5
MsG0480023138.01.T01	AT1G58520	58.333	48	19	1	5	51	34	81	3.09e-11	60.1
MsG0480023138.01.T01	AT1G58525	58.333	48	19	1	5	51	34	81	3.09e-11	60.1
MsG0480023138.01.T01	AT2G42990	40.206	97	36	2	5	79	32	128	6.03e-11	59.3
MsG0480023138.01.T01	AT1G75930	38.614	101	39	2	3	80	31	131	6.36e-11	59.3

Find 34 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTAGTTGGATTAGGCCTATT+AGG	0.251903	4:-81320899	MsG0480023138.01.T01:CDS
AAAGTTAATTACAGGCCTTA+TGG	0.291310	4:-81322951	MsG0480023138.01.T01:CDS
AAGTTAATTACAGGCCTTAT+GGG	0.326292	4:-81322950	MsG0480023138.01.T01:CDS
CACTGTTAATGAAGATGAAT+TGG	0.336356	4:+81320742	None:intergenic
CCGATGCCAAAGTTAATTAC+AGG	0.368345	4:-81322959	MsG0480023138.01.T01:CDS
CATGTGGAACGCAACCTAAT+AGG	0.400237	4:+81320885	None:intergenic
GCAGGGATCCGCAATGAGAC+TGG	0.431672	4:-81322434	MsG0480023138.01.T01:CDS
AATTATGCATCCGGTGCAGC+AGG	0.432883	4:-81322452	MsG0480023138.01.T01:CDS
ATAAGGCCTGTAATTAACTT+TGG	0.440046	4:+81322953	None:intergenic
GAGCAGGCTATACATGGGCT+TGG	0.450690	4:-81320938	MsG0480023138.01.T01:intron
CAAGGTGTTAATTATGCATC+CGG	0.468458	4:-81322461	MsG0480023138.01.T01:CDS
ATTGCGGATCCCTGCTGCAC+CGG	0.476096	4:+81322442	None:intergenic
GTGCATGAGCAGGCTATACA+TGG	0.486706	4:-81320944	MsG0480023138.01.T01:intron
TAACTTTGGCATCGGTAGCT+AGG	0.487931	4:+81322967	None:intergenic
AATCTTCCCGTAGGACCAGC+AGG	0.519591	4:+81322918	None:intergenic
CCACATGAAATTTCTACCCA+TGG	0.524099	4:-81320869	MsG0480023138.01.T01:CDS
CAATGAGACTGGAACACACT+TGG	0.529479	4:-81322423	MsG0480023138.01.T01:intron
GCAGGAAAATCAATCCCATA+AGG	0.529895	4:+81322936	None:intergenic
CATGGTGCAGACATAGTTCA+AGG	0.533410	4:-81322479	MsG0480023138.01.T01:intron
CACATGAAATTTCTACCCAT+GGG	0.551621	4:-81320868	MsG0480023138.01.T01:CDS
CCTGTAATTAACTTTGGCAT+CGG	0.559604	4:+81322959	None:intergenic
GTCAACTGAAGTACGGCCAT+TGG	0.569497	4:+81322893	None:intergenic
TGATTATGTCAACTGAAGTA+CGG	0.593606	4:+81322886	None:intergenic
CCATGGGTAGAAATTTCATG+TGG	0.599051	4:+81320869	None:intergenic
ATCTCCAAAATGAAAAGACA+TGG	0.607075	4:+81323019	None:intergenic
TTTATTCAAACGATCAACGA+GGG	0.616697	4:+81320778	None:intergenic
TGATTCAAGGTGTATTCAAG+AGG	0.623109	4:-81320840	MsG0480023138.01.T01:CDS
GGAGATTCTTTATCGGACAG+TGG	0.630401	4:-81323002	MsG0480023138.01.T01:CDS
CTTTATTCAAACGATCAACG+AGG	0.650020	4:+81320777	None:intergenic
ATTATGCATCCGGTGCAGCA+GGG	0.675210	4:-81322451	MsG0480023138.01.T01:CDS
CCTACGGGAAGATTTACCAA+TGG	0.686839	4:-81322909	MsG0480023138.01.T01:CDS
CCATTGGTAAATCTTCCCGT+AGG	0.687811	4:+81322909	None:intergenic
TGCATGAGCAGGCTATACAT+GGG	0.691621	4:-81320943	MsG0480023138.01.T01:intron
AGTGTGTTCCAGTCTCATTG+CGG	0.749177	4:+81322426	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGAATTATCAACTTATTTAA+AGG	-	Chr4:81321742-81321761	MsG0480023138.01.T01:intron	15.0%
!!!	CGTAATTCTTAAATTTTTAT+TGG	-	Chr4:81321989-81322008	MsG0480023138.01.T01:intron	15.0%
!!!	TTAAATAAGTTGATAATTCT+TGG	+	Chr4:81321742-81321761	None:intergenic	15.0%
!!	AGAAAATCAACTAAATAGAA+AGG	+	Chr4:81322043-81322062	None:intergenic	20.0%
!!	ATGAAATCCAAAAAAATGTA+AGG	-	Chr4:81322560-81322579	MsG0480023138.01.T01:intron	20.0%
!!	CTAAATAGAAAGGTAAAAAA+TGG	+	Chr4:81322033-81322052	None:intergenic	20.0%
!!	CTGATACAATATTGTATATT+AGG	-	Chr4:81322140-81322159	MsG0480023138.01.T01:intron	20.0%
!!	GAAAATATGTTTATAAGTTG+CGG	-	Chr4:81320951-81320970	MsG0480023138.01.T01:intron	20.0%
!!	TGTAAGATTTGAATTAAGTT+CGG	-	Chr4:81320994-81321013	MsG0480023138.01.T01:intron	20.0%
!!	TGTTTGTATTATGTGAATAT+AGG	-	Chr4:81321626-81321645	MsG0480023138.01.T01:intron	20.0%
!!	TTAGATTCAAATTTAGGAAT+TGG	-	Chr4:81321054-81321073	MsG0480023138.01.T01:intron	20.0%
!!!	AGTAGATTTTAAGAATAATC+GGG	-	Chr4:81322374-81322393	MsG0480023138.01.T01:intron	20.0%
!!!	ATGGACTATTATGTTATATT+GGG	-	Chr4:81322115-81322134	MsG0480023138.01.T01:intron	20.0%
!!!	CTTTTTAACACACTTTTTAT+TGG	+	Chr4:81321957-81321976	None:intergenic	20.0%
!!!	GAGTAGATTTTAAGAATAAT+CGG	-	Chr4:81322373-81322392	MsG0480023138.01.T01:intron	20.0%
!!!	TAGTTGATTTTCTTTTGTTT+AGG	-	Chr4:81322050-81322069	MsG0480023138.01.T01:intron	20.0%
!!!	TATGGACTATTATGTTATAT+TGG	-	Chr4:81322114-81322133	MsG0480023138.01.T01:intron	20.0%
!!!	TCATTCTTTAATCAACTTTT+TGG	-	Chr4:81321166-81321185	MsG0480023138.01.T01:intron	20.0%
!!!	TTTCCTTGTTATCAAAATTT+AGG	+	Chr4:81321104-81321123	None:intergenic	20.0%
!!!	TTTTTAGTTTTTTTTGGGAT+TGG	-	Chr4:81322091-81322110	MsG0480023138.01.T01:intron	20.0%
!	AAATCCCGCTAAATTTATTT+GGG	+	Chr4:81321924-81321943	None:intergenic	25.0%
!	ATAATCCGAACTAAATGTAA+AGG	+	Chr4:81322542-81322561	None:intergenic	25.0%
!	ATCTTACAAAGACATGTATA+AGG	+	Chr4:81321462-81321481	None:intergenic	25.0%
!	ATGAATGAATTATCACATGT+AGG	-	Chr4:81322688-81322707	MsG0480023138.01.T01:intron	25.0%
!	CCTCAAAAACTAATTTAACT+GGG	+	Chr4:81321832-81321851	None:intergenic	25.0%
!	CTCAAAAACTAATTTAACTG+GGG	+	Chr4:81321831-81321850	None:intergenic	25.0%
!	GAATCAATTTGAATTTCAAC+TGG	+	Chr4:81321885-81321904	None:intergenic	25.0%
!	GCAATCTTAGATTCAAATTT+AGG	-	Chr4:81321048-81321067	MsG0480023138.01.T01:intron	25.0%
!	TAAATCCCGCTAAATTTATT+TGG	+	Chr4:81321925-81321944	None:intergenic	25.0%
!	TAAGATTAATTAAGCACCTT+TGG	-	Chr4:81321026-81321045	MsG0480023138.01.T01:intron	25.0%
!	TAATCCGAACTAAATGTAAA+GGG	+	Chr4:81322541-81322560	None:intergenic	25.0%
!	TACATGTGATAATTCATTCA+TGG	+	Chr4:81322689-81322708	None:intergenic	25.0%
!	TAGATCCCAAATAAATTTAG+CGG	-	Chr4:81321916-81321935	MsG0480023138.01.T01:intron	25.0%
!	TATATGCCATTAGATCATAT+TGG	-	Chr4:81322435-81322454	MsG0480023138.01.T01:CDS	25.0%
!	TCACAAATTATTAGTCACTT+TGG	-	Chr4:81321486-81321505	MsG0480023138.01.T01:intron	25.0%
!	TCGTCCAATATAAAATTTGT+CGG	+	Chr4:81320921-81320940	None:intergenic	25.0%
!	TTTCAAAAGTTAGAACCATT+TGG	-	Chr4:81321799-81321818	MsG0480023138.01.T01:intron	25.0%
!!	AAGCTTCCAAAGTAATTTTA+GGG	+	Chr4:81322182-81322201	None:intergenic	25.0%
!!	AAGTTTTCTTTAGTTGGATT+AGG	-	Chr4:81322842-81322861	MsG0480023138.01.T01:intron	25.0%
!!	TAAAATTACTTTGGAAGCTT+GGG	-	Chr4:81322182-81322201	MsG0480023138.01.T01:intron	25.0%
!!!	AAAAAAATGTAAGGTGTTGA+AGG	-	Chr4:81322569-81322588	MsG0480023138.01.T01:intron	25.0%
!!!	AAAAAATGTAAGGTGTTGAA+GGG	-	Chr4:81322570-81322589	MsG0480023138.01.T01:intron	25.0%
!!!	AAGCTCCCTTTTTTTTTTTT+GGG	-	Chr4:81322404-81322423	MsG0480023138.01.T01:intron	25.0%
!!!	CCAGTTAAATTAGTTTTTGA+GGG	-	Chr4:81321830-81321849	MsG0480023138.01.T01:intron	25.0%
!!!	GTAGATTTTAAGAATAATCG+GGG	-	Chr4:81322375-81322394	MsG0480023138.01.T01:intron	25.0%
!!!	TCATTTTGGAGATTCTTTAT+CGG	-	Chr4:81320741-81320760	MsG0480023138.01.T01:CDS	25.0%
!!!	TCTCCTAAATTTTGATAACA+AGG	-	Chr4:81321098-81321117	MsG0480023138.01.T01:intron	25.0%
!!!	TTCAACACCTTACATTTTTT+TGG	+	Chr4:81322570-81322589	None:intergenic	25.0%
	AAAAACTAATTTAACTGGGG+CGG	+	Chr4:81321828-81321847	None:intergenic	30.0%
	AAAGTTAATTACAGGCCTTA+TGG	-	Chr4:81320799-81320818	MsG0480023138.01.T01:CDS	30.0%
	AAGTGAGTGTATACTATAAG+TGG	+	Chr4:81322663-81322682	None:intergenic	30.0%
	AATCCCCCCAAAAAAAAAAA+AGG	+	Chr4:81322413-81322432	None:intergenic	30.0%
	ACATCCAGATGAATAAATCT+GGG	-	Chr4:81322348-81322367	MsG0480023138.01.T01:intron	30.0%
	AGATCCCAAATAAATTTAGC+GGG	-	Chr4:81321917-81321936	MsG0480023138.01.T01:intron	30.0%
	ATAAGGCCTGTAATTAACTT+TGG	+	Chr4:81320800-81320819	None:intergenic	30.0%
	ATCCCCCCAAAAAAAAAAAA+GGG	+	Chr4:81322412-81322431	None:intergenic	30.0%
	CACTGTTAATGAAGATGAAT+TGG	+	Chr4:81323011-81323030	None:intergenic	30.0%
	CCCTCAAAAACTAATTTAAC+TGG	+	Chr4:81321833-81321852	None:intergenic	30.0%
	TAGAGACCAATATGATCTAA+TGG	+	Chr4:81322444-81322463	None:intergenic	30.0%
	TCCAAAATTGCTAAGAAACT+TGG	-	Chr4:81321574-81321593	MsG0480023138.01.T01:intron	30.0%
	TGATTATGTCAACTGAAGTA+CGG	+	Chr4:81320867-81320886	None:intergenic	30.0%
	TTTATTCAAACGATCAACGA+GGG	+	Chr4:81322975-81322994	None:intergenic	30.0%
	TTTCCCTATTCGAAATTACA+GGG	+	Chr4:81322311-81322330	None:intergenic	30.0%
!	AAGTTAATTACAGGCCTTAT+GGG	-	Chr4:81320800-81320819	MsG0480023138.01.T01:CDS	30.0%
!	AGCTTCCAAAGTAATTTTAG+GGG	+	Chr4:81322181-81322200	None:intergenic	30.0%
!	CAAGCTTCCAAAGTAATTTT+AGG	+	Chr4:81322183-81322202	None:intergenic	30.0%
!	CATGCCGACAAATTTTATAT+TGG	-	Chr4:81320914-81320933	MsG0480023138.01.T01:CDS	30.0%
!	CTAAAATTACTTTGGAAGCT+TGG	-	Chr4:81322181-81322200	MsG0480023138.01.T01:intron	30.0%
!	GCAAGAAAGTTTTCTTTAGT+TGG	-	Chr4:81322836-81322855	MsG0480023138.01.T01:intron	30.0%
!	TCCAAGTTTCTTAGCAATTT+TGG	+	Chr4:81321578-81321597	None:intergenic	30.0%
!	TTTGAATCTAAGATTGCCAA+AGG	+	Chr4:81321045-81321064	None:intergenic	30.0%
!	TTTTCCCTATTCGAAATTAC+AGG	+	Chr4:81322312-81322331	None:intergenic	30.0%
!!!	AGCTCCCTTTTTTTTTTTTG+GGG	-	Chr4:81322405-81322424	MsG0480023138.01.T01:intron	30.0%
!!!	AGTTTTTTTTGGGATTGGTA+TGG	-	Chr4:81322096-81322115	MsG0480023138.01.T01:intron	30.0%
!!!	CCCAGTTAAATTAGTTTTTG+AGG	-	Chr4:81321829-81321848	MsG0480023138.01.T01:intron	30.0%
!!!	GAAGCTCCCTTTTTTTTTTT+TGG	-	Chr4:81322403-81322422	MsG0480023138.01.T01:intron	30.0%
!!!	TTTTTTTTTTTTGGGGGGAT+TGG	-	Chr4:81322412-81322431	MsG0480023138.01.T01:intron	30.0%
	AGCTGCAATAGTAGAATTCT+TGG	+	Chr4:81321207-81321226	None:intergenic	35.0%
	CAAACCCCCTAAAATTACTT+TGG	-	Chr4:81322173-81322192	MsG0480023138.01.T01:intron	35.0%
	CACATCCAGATGAATAAATC+TGG	-	Chr4:81322347-81322366	MsG0480023138.01.T01:intron	35.0%
	CACATGAAATTTCTACCCAT+GGG	-	Chr4:81322882-81322901	MsG0480023138.01.T01:intron	35.0%
	CCACAACATTATCCAACTAA+GGG	-	Chr4:81321680-81321699	MsG0480023138.01.T01:intron	35.0%
	CCTGTAATTAACTTTGGCAT+CGG	+	Chr4:81320794-81320813	None:intergenic	35.0%
	CTTTATTCAAACGATCAACG+AGG	+	Chr4:81322976-81322995	None:intergenic	35.0%
	TAGGAGTTTACATCACTTGT+AGG	-	Chr4:81322707-81322726	MsG0480023138.01.T01:intron	35.0%
	TGATTCAAGGTGTATTCAAG+AGG	-	Chr4:81322910-81322929	MsG0480023138.01.T01:CDS	35.0%
	TTCCCTATTCGAAATTACAG+GGG	+	Chr4:81322310-81322329	None:intergenic	35.0%
	TTTAAGAATAATCGGGGTGT+AGG	-	Chr4:81322381-81322400	MsG0480023138.01.T01:intron	35.0%
	TTTGCATAGGTGGGATAAAA+TGG	+	Chr4:81321247-81321266	None:intergenic	35.0%
!	ACTATTGCAGCTGAACTTTT+GGG	-	Chr4:81321215-81321234	MsG0480023138.01.T01:intron	35.0%
!	CAAGGTGTTAATTATGCATC+CGG	-	Chr4:81321289-81321308	MsG0480023138.01.T01:intron	35.0%
!	GCTTCCAAAGTAATTTTAGG+GGG	+	Chr4:81322180-81322199	None:intergenic	35.0%
!	TACTATTGCAGCTGAACTTT+TGG	-	Chr4:81321214-81321233	MsG0480023138.01.T01:intron	35.0%
!	TTAGTTGGATTAGGCCTATT+AGG	-	Chr4:81322851-81322870	MsG0480023138.01.T01:intron	35.0%
!!	ACCTTGAATCATTTTTCCCA+TGG	+	Chr4:81322901-81322920	None:intergenic	35.0%
!!	CCTTGAATCATTTTTCCCAT+GGG	+	Chr4:81322900-81322919	None:intergenic	35.0%
!!!	CTCCCTTTTTTTTTTTTGGG+GGG	-	Chr4:81322407-81322426	MsG0480023138.01.T01:intron	35.0%
!!!	GCTCCCTTTTTTTTTTTTGG+GGG	-	Chr4:81322406-81322425	MsG0480023138.01.T01:intron	35.0%
	ACACCCCTGTAATTTCGAAT+AGG	-	Chr4:81322304-81322323	MsG0480023138.01.T01:intron	40.0%
	CACCCCTGTAATTTCGAATA+GGG	-	Chr4:81322305-81322324	MsG0480023138.01.T01:intron	40.0%
	CCACATGAAATTTCTACCCA+TGG	-	Chr4:81322881-81322900	MsG0480023138.01.T01:intron	40.0%
	CCATGGGTAGAAATTTCATG+TGG	+	Chr4:81322884-81322903	None:intergenic	40.0%
	CCCATGGGAAAAATGATTCA+AGG	-	Chr4:81322897-81322916	MsG0480023138.01.T01:CDS	40.0%
	CCCTTAGTTGGATAATGTTG+TGG	+	Chr4:81321683-81321702	None:intergenic	40.0%
	CCGATGCCAAAGTTAATTAC+AGG	-	Chr4:81320791-81320810	MsG0480023138.01.T01:CDS	40.0%
	CGCACCCTTTACATTTAGTT+CGG	-	Chr4:81322534-81322553	MsG0480023138.01.T01:intron	40.0%
	GCAGGAAAATCAATCCCATA+AGG	+	Chr4:81320817-81320836	None:intergenic	40.0%
	GCCACAACATTATCCAACTA+AGG	-	Chr4:81321679-81321698	MsG0480023138.01.T01:intron	40.0%
	GTAGTGTACTTTCCCTTAGT+TGG	+	Chr4:81321695-81321714	None:intergenic	40.0%
	TAGTTGGATAATGTTGTGGC+AGG	+	Chr4:81321679-81321698	None:intergenic	40.0%
	TCCACCCAGATTTATTCATC+TGG	+	Chr4:81322355-81322374	None:intergenic	40.0%
	TCCAGATGAATAAATCTGGG+TGG	-	Chr4:81322351-81322370	MsG0480023138.01.T01:intron	40.0%
	TCTACAGTAAAAGTCCAGCA+TGG	+	Chr4:81322470-81322489	None:intergenic	40.0%
	TTACTGTAGAACCCAGTTAG+TGG	-	Chr4:81322480-81322499	MsG0480023138.01.T01:intron	40.0%
	TTGAACTATGTCTGCACCAT+GGG	+	Chr4:81321272-81321291	None:intergenic	40.0%
	TTGGGTAGTTGTTGAGTTAG+AGG	-	Chr4:81322200-81322219	MsG0480023138.01.T01:intron	40.0%
!	AGAGTGCTTGAGAAACTAGT+AGG	-	Chr4:81322618-81322637	MsG0480023138.01.T01:intron	40.0%
!	ATTGGTCTCTAATACCATGC+TGG	-	Chr4:81322453-81322472	MsG0480023138.01.T01:CDS	40.0%
!!	ACTTTGGCAATGTTGATTCC+AGG	-	Chr4:81321502-81321521	MsG0480023138.01.T01:intron	40.0%
!!	CTTTGGCAATGTTGATTCCA+GGG	-	Chr4:81321503-81321522	MsG0480023138.01.T01:intron	40.0%
!!	TATGGGATTGATTTTCCTGC+TGG	-	Chr4:81320817-81320836	MsG0480023138.01.T01:CDS	40.0%
	ACCATGGGTATTTGCATAGG+TGG	+	Chr4:81321257-81321276	None:intergenic	45.0%
	ACCCAGTTAGTGGAGTAATG+CGG	-	Chr4:81322490-81322509	MsG0480023138.01.T01:intron	45.0%
	ACCGCATTACTCCACTAACT+GGG	+	Chr4:81322494-81322513	None:intergenic	45.0%
	AGTGTGTTCCAGTCTCATTG+CGG	+	Chr4:81321327-81321346	None:intergenic	45.0%
	ATTTGTGTGAGTGCATGAGC+AGG	-	Chr4:81322796-81322815	MsG0480023138.01.T01:intron	45.0%
	CAATGAGACTGGAACACACT+TGG	-	Chr4:81321327-81321346	MsG0480023138.01.T01:intron	45.0%
	CATGGTGCAGACATAGTTCA+AGG	-	Chr4:81321271-81321290	MsG0480023138.01.T01:intron	45.0%
	CATGTGGAACGCAACCTAAT+AGG	+	Chr4:81322868-81322887	None:intergenic	45.0%
	CCATGGGTATTTGCATAGGT+GGG	+	Chr4:81321256-81321275	None:intergenic	45.0%
	CCATTGGTAAATCTTCCCGT+AGG	+	Chr4:81320844-81320863	None:intergenic	45.0%
	CCTACGGGAAGATTTACCAA+TGG	-	Chr4:81320841-81320860	MsG0480023138.01.T01:CDS	45.0%
	CTTGAACTATGTCTGCACCA+TGG	+	Chr4:81321273-81321292	None:intergenic	45.0%
	GGAGATTCTTTATCGGACAG+TGG	-	Chr4:81320748-81320767	MsG0480023138.01.T01:CDS	45.0%
	GTGGCAGGAGGTAATTGTTA+AGG	+	Chr4:81321664-81321683	None:intergenic	45.0%
	TGCACCATGGGTATTTGCAT+AGG	+	Chr4:81321260-81321279	None:intergenic	45.0%
	TGCATGAGCAGGCTATACAT+GGG	-	Chr4:81322807-81322826	MsG0480023138.01.T01:intron	45.0%
	TTGGATAATGTTGTGGCAGG+AGG	+	Chr4:81321676-81321695	None:intergenic	45.0%
!	TGATTTTCCTGCTGGTCCTA+CGG	-	Chr4:81320825-81320844	MsG0480023138.01.T01:CDS	45.0%
!!	TAACTTTGGCATCGGTAGCT+AGG	+	Chr4:81320786-81320805	None:intergenic	45.0%
!!!	ATTATATTTATCTTAAATTT+TGG	-	Chr4:81321374-81321393	MsG0480023138.01.T01:intron	5.0%
!!!	ATTTATTTTTAGTTTTTTTT+GGG	-	Chr4:81322086-81322105	MsG0480023138.01.T01:intron	5.0%
!!!	TATTTATTTTTAGTTTTTTT+TGG	-	Chr4:81322085-81322104	MsG0480023138.01.T01:intron	5.0%
	AATTATGCATCCGGTGCAGC+AGG	-	Chr4:81321298-81321317	MsG0480023138.01.T01:intron	50.0%
	AGGGTCCAGATATCAGCATG+GGG	-	Chr4:81321522-81321541	MsG0480023138.01.T01:intron	50.0%
	ATTATGCATCCGGTGCAGCA+GGG	-	Chr4:81321299-81321318	MsG0480023138.01.T01:intron	50.0%
	CAGGGTCCAGATATCAGCAT+GGG	-	Chr4:81321521-81321540	MsG0480023138.01.T01:intron	50.0%
	CCCACCTATGCAAATACCCA+TGG	-	Chr4:81321253-81321272	MsG0480023138.01.T01:intron	50.0%
	GACCGCATTACTCCACTAAC+TGG	+	Chr4:81322495-81322514	None:intergenic	50.0%
	GTCAACTGAAGTACGGCCAT+TGG	+	Chr4:81320860-81320879	None:intergenic	50.0%
	GTGCATGAGCAGGCTATACA+TGG	-	Chr4:81322806-81322825	MsG0480023138.01.T01:intron	50.0%
	TGCAACCCCATGCTGATATC+TGG	+	Chr4:81321530-81321549	None:intergenic	50.0%
!	GATTTTCCTGCTGGTCCTAC+GGG	-	Chr4:81320826-81320845	MsG0480023138.01.T01:CDS	50.0%
	AATCTTCCCGTAGGACCAGC+AGG	+	Chr4:81320835-81320854	None:intergenic	55.0%
	CCAGGGTCCAGATATCAGCA+TGG	-	Chr4:81321520-81321539	MsG0480023138.01.T01:intron	55.0%
	CCATGCTGATATCTGGACCC+TGG	+	Chr4:81321523-81321542	None:intergenic	55.0%
	GAGCAGGCTATACATGGGCT+TGG	-	Chr4:81322812-81322831	MsG0480023138.01.T01:intron	55.0%
	TAACTGGGGCGGAGTCCAAA+TGG	+	Chr4:81321817-81321836	None:intergenic	55.0%
	ATTGCGGATCCCTGCTGCAC+CGG	+	Chr4:81321311-81321330	None:intergenic	60.0%
	GCAGGGATCCGCAATGAGAC+TGG	-	Chr4:81321316-81321335	MsG0480023138.01.T01:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	81320733	81323039	81320733	ID=MsG0480023138.01;Name=MsG0480023138.01
Chr4	mRNA	81320733	81323039	81320733	ID=MsG0480023138.01.T01;Parent=MsG0480023138.01;Name=MsG0480023138.01.T01;_AED=0.46;_eAED=0.47;_QI=0\|0\|0\|1\|1\|1\|3\|0\|147
Chr4	exon	81322886	81323039	81322886	ID=MsG0480023138.01.T01:exon:5220;Parent=MsG0480023138.01.T01
Chr4	exon	81322424	81322491	81322424	ID=MsG0480023138.01.T01:exon:5219;Parent=MsG0480023138.01.T01
Chr4	exon	81320733	81320954	81320733	ID=MsG0480023138.01.T01:exon:5218;Parent=MsG0480023138.01.T01
Chr4	CDS	81322886	81323039	81322886	ID=MsG0480023138.01.T01:cds;Parent=MsG0480023138.01.T01
Chr4	CDS	81322424	81322491	81322424	ID=MsG0480023138.01.T01:cds;Parent=MsG0480023138.01.T01
Chr4	CDS	81320733	81320954	81320733	ID=MsG0480023138.01.T01:cds;Parent=MsG0480023138.01.T01

Gene Sequence

>MsG0480023138.01.T01

ATGTCTTTTCATTTTGGAGATTCTTTATCGGACAGTGGAAACAACAATAACCTAGCTACCGATGCCAAAGTTAATTACAGGCCTTATGGGATTGATTTTCCTGCTGGTCCTACGGGAAGATTTACCAATGGCCGTACTTCAGTTGACATAATCAACATAGTTCAAGGTGTTAATTATGCATCCGGTGCAGCAGGGATCCGCAATGAGACTGGAACACACTTGGCTATACATGGGCTTGGAGCAAGAAAGTTTTCTTTAGTTGGATTAGGCCTATTAGGTTGCGTTCCACATGAAATTTCTACCCATGGGAAAAATGATTCAAGGTGTATTCAAGAGGAGAATAATGCAGCACTACTTTTCAATGACAAACTTAAAGCCCTCGTTGATCGTTTGAATAAAGAATTAACTGATTCCAATTCATCTTCATTAACAGTGCTGTCATAA

Protein sequence

>MsG0480023138.01.T01

MSFHFGDSLSDSGNNNNLATDAKVNYRPYGIDFPAGPTGRFTNGRTSVDIINIVQGVNYASGAAGIRNETGTHLAIHGLGARKFSLVGLGLLGCVPHEISTHGKNDSRCIQEENNAALLFNDKLKALVDRLNKELTDSNSSSLTVLS*