AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023327.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023327.01.T01 MTR_8g090275 98.249 514 9 0 1 514 1 514 0.0 1027
MsG0480023327.01.T01 MTR_8g090285 79.457 516 101 3 1 514 2 514 0.0 832
MsG0480023327.01.T01 MTR_8g090270 76.641 518 82 4 1 514 2 484 0.0 730
MsG0480023327.01.T01 MTR_5g063390 71.069 477 134 1 1 473 1 477 0.0 690
MsG0480023327.01.T01 MTR_5g063390 65.517 348 116 1 130 473 1 348 1.34e-163 469
MsG0480023327.01.T01 MTR_5g019490 41.901 463 251 3 5 455 38 494 2.31e-110 337
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023327.01.T01 AT5G10190 69.485 485 143 2 1 480 1 485 0.0 684
MsG0480023327.01.T01 AT1G78130 70.064 471 138 1 1 468 1 471 0.0 658
MsG0480023327.01.T01 AT5G10190 71.594 345 98 0 1 345 1 345 1.19e-175 498
MsG0480023327.01.T01 AT5G10190 64.326 356 122 2 130 480 1 356 1.13e-163 468
MsG0480023327.01.T01 AT4G36790 42.826 453 251 4 5 455 42 488 1.31e-119 360
MsG0480023327.01.T01 AT2G18590 38.126 459 264 7 12 455 19 472 1.37e-96 301

Find 120 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTCCTGTTGTTGGATTAT+TGG 0.114248 4:-83758749 MsG0480023327.01.T01:CDS
TTTATAGTTTCTGCTTCATT+TGG 0.212769 4:-83759308 MsG0480023327.01.T01:CDS
CATCTCCTCCACCTTCTTAC+AGG 0.213357 4:-83760154 MsG0480023327.01.T01:intron
GGAACTATTTCTGCAAATAT+TGG 0.230215 4:+83758838 None:intergenic
GGAAACCTTGGAAGTATTAT+TGG 0.246283 4:-83759689 MsG0480023327.01.T01:CDS
ACATCAATCTTTGGTATATC+TGG 0.252770 4:-83759638 MsG0480023327.01.T01:CDS
GCAATTTCAAGAGGTTTAAA+TGG 0.266877 4:-83759812 MsG0480023327.01.T01:CDS
GTTTGTCATTTGCCACTCTT+TGG 0.291019 4:-83759382 MsG0480023327.01.T01:CDS
GCTTCATTTGGGTCTCTGTT+TGG 0.295240 4:-83759296 MsG0480023327.01.T01:CDS
TCATTTGCTCCTCCTGTTGT+TGG 0.303303 4:-83758757 MsG0480023327.01.T01:CDS
ATGGTACAACTGCAATTCTT+TGG 0.304541 4:-83759337 MsG0480023327.01.T01:CDS
TCTTTACCATTCAGTTCATT+TGG 0.312746 4:+83758442 None:intergenic
ACGGAATCAGAAGATCCTTT+TGG 0.318779 4:+83758697 None:intergenic
AACATTATTCTTCCGGTGTT+TGG 0.335561 4:+83759239 None:intergenic
TAGTGTTATAGTTGGTATAT+TGG 0.339062 4:-83759582 MsG0480023327.01.T01:CDS
GTTAGTTGCCGGAGCATTCC+AGG 0.341774 4:+83759094 None:intergenic
AGCAACCGTGGTGTGGCATT+TGG 0.346177 4:-83759728 MsG0480023327.01.T01:CDS
TTATAGTTTCTGCTTCATTT+GGG 0.358433 4:-83759307 MsG0480023327.01.T01:CDS
TTGTGACAACATTATTCTTC+CGG 0.360186 4:+83759232 None:intergenic
AGCAAGAATGGTTGCATTAG+AGG 0.370278 4:-83758542 MsG0480023327.01.T01:CDS
AGTACATACTTGTTAGTTGC+CGG 0.383635 4:+83759083 None:intergenic
AACTCTATGTTCTCTTCCCT+TGG 0.389261 4:+83758388 None:intergenic
CACAGCCTTCATGCATGGTC+TGG 0.391306 4:-83759144 MsG0480023327.01.T01:CDS
TTTGGATCATTCCCATGGTC+AGG 0.395458 4:-83759404 MsG0480023327.01.T01:CDS
GCAACTAGAGACTGAATTGC+TGG 0.405446 4:+83759767 None:intergenic
CTAGCCTCAACATCAATCTT+TGG 0.405866 4:-83759647 MsG0480023327.01.T01:CDS
GCAACCGTGGTGTGGCATTT+GGG 0.418380 4:-83759727 MsG0480023327.01.T01:CDS
CCGGACCGCTATATATGCTT+TGG 0.423157 4:-83758806 MsG0480023327.01.T01:CDS
TTGGGTCTCTGTTTGGAGGA+TGG 0.423181 4:-83759289 MsG0480023327.01.T01:CDS
ACTCTTTGGTTAGAACTGAT+AGG 0.425663 4:-83759368 MsG0480023327.01.T01:CDS
TTTGGGTGGCTACAACTAAC+AGG 0.432702 4:-83759710 MsG0480023327.01.T01:CDS
GTTTCAGATAATTGTGGCTC+AGG 0.441621 4:-83759432 MsG0480023327.01.T01:CDS
TGTGCCAATAATCCAACAAC+AGG 0.446669 4:+83758745 None:intergenic
TCAAGAGGTTTAAATGGCAT+TGG 0.449252 4:-83759806 MsG0480023327.01.T01:CDS
CCGTTGAAATCGAAACAGAT+AGG 0.449263 4:-83758678 MsG0480023327.01.T01:CDS
CTTGCTCGATCTCTGTCTCT+CGG 0.450052 4:+83758559 None:intergenic
TCATTTGGGTCTCTGTTTGG+AGG 0.454615 4:-83759293 MsG0480023327.01.T01:CDS
AGGAGCAAATGATTGCAATA+TGG 0.459790 4:+83758768 None:intergenic
AGACAGAGATCGAGCAAGAA+TGG 0.461109 4:-83758554 MsG0480023327.01.T01:CDS
AACGCTGATCCAACTGCGTT+AGG 0.461170 4:-83760312 MsG0480023327.01.T01:CDS
ATAGCGGTCCGGGACTTCTC+AGG 0.464308 4:+83758817 None:intergenic
AAGGCTGTGGATGGATCATC+AGG 0.468694 4:+83759158 None:intergenic
CACACCACGGTTGCTATCAA+TGG 0.472389 4:+83759736 None:intergenic
TCTCTGTTTGGAGGATGGTT+CGG 0.476250 4:-83759284 MsG0480023327.01.T01:CDS
TCAATCGTTCTGTTATCCTC+TGG 0.481918 4:-83760262 MsG0480023327.01.T01:CDS
CTGTAAGAAGGTGGAGGAGA+TGG 0.486917 4:+83760155 None:intergenic
CGTGCACATGTTATTGCTCT+AGG 0.489987 4:-83760213 MsG0480023327.01.T01:CDS
CCAAAGCATATATAGCGGTC+CGG 0.491618 4:+83758806 None:intergenic
GGCTATAGACCAGTTCCAAA+AGG 0.492838 4:-83758712 MsG0480023327.01.T01:CDS
TTAGAACCAAATGAACTGAA+TGG 0.503151 4:-83758448 MsG0480023327.01.T01:CDS
TCATGGGATTTGCTACATCC+TGG 0.505406 4:-83759112 MsG0480023327.01.T01:CDS
TTGCTCGATCTCTGTCTCTC+GGG 0.510352 4:+83758560 None:intergenic
CTCTCGGGTATGTGCAGTAA+AGG 0.511918 4:+83758575 None:intergenic
TCCGGTGTTTGGTAACCGTA+GGG 0.514777 4:+83759250 None:intergenic
TTGATTCAGAATAAAAGGAC+TGG 0.516795 4:+83759498 None:intergenic
AACAGCAGAATGGCTGCTAA+AGG 0.518585 4:+83759188 None:intergenic
TGCTACATCCTGGAATGCTC+CGG 0.525885 4:-83759102 MsG0480023327.01.T01:CDS
CCAACTTCTTTGTATACTCC+TGG 0.527749 4:+83760345 None:intergenic
GTAAATAAGAACCTGTAAGA+AGG 0.528322 4:+83760143 None:intergenic
CAATCTTTGGTATATCTGGT+TGG 0.529034 4:-83759634 MsG0480023327.01.T01:CDS
ATGATGATTTAGATTATCCA+AGG 0.531255 4:-83758405 MsG0480023327.01.T01:CDS
CCTATCTGTTTCGATTTCAA+CGG 0.536774 4:+83758678 None:intergenic
GCTGATGAGTCATTGCTGCC+AGG 0.538215 4:-83760363 MsG0480023327.01.T01:CDS
TGAAGCAGAAACTATAAAGA+AGG 0.539808 4:+83759313 None:intergenic
CAAAGCATATATAGCGGTCC+GGG 0.540208 4:+83758807 None:intergenic
GCATTGATTAGTGTTATAGT+TGG 0.540633 4:-83759590 MsG0480023327.01.T01:CDS
CGTTGAAATCGAAACAGATA+GGG 0.541194 4:-83758677 MsG0480023327.01.T01:CDS
AAATAGTTCCTGAGAAGTCC+CGG 0.542292 4:-83758825 MsG0480023327.01.T01:CDS
CCTTGCATATATGAACTATC+GGG 0.548221 4:+83758336 None:intergenic
GCCACAATTATCTGAAACGA+TGG 0.552039 4:+83759437 None:intergenic
TCGGAGATTACTTATCCCTA+CGG 0.552310 4:-83759265 MsG0480023327.01.T01:CDS
TATACCAAAGATTGATGTTG+AGG 0.552826 4:+83759643 None:intergenic
AACCTTGGAAGTATTATTGG+TGG 0.556204 4:-83759686 MsG0480023327.01.T01:CDS
TTTAATAAAAGAAGCAAAAG+CGG 0.556479 4:-83759471 MsG0480023327.01.T01:CDS
GTGAGGGAACCTAACGCAGT+TGG 0.559692 4:+83760303 None:intergenic
ATGCAGACACAAAGTGTCAA+AGG 0.562578 4:+83758607 None:intergenic
CCAGGAGTATACAAAGAAGT+TGG 0.569677 4:-83760345 MsG0480023327.01.T01:CDS
TTCCGGTGTTTGGTAACCGT+AGG 0.572409 4:+83759249 None:intergenic
CACGATTGTGTCGCGTGGCG+AGG 0.573750 4:+83760232 None:intergenic
CTACAACTAACAGGAAACCT+TGG 0.573919 4:-83759701 MsG0480023327.01.T01:CDS
CCATCCACAGCCTTCATGCA+TGG 0.574542 4:-83759149 MsG0480023327.01.T01:CDS
CCATGCATGAAGGCTGTGGA+TGG 0.574600 4:+83759149 None:intergenic
ATGTTGTCACAAATAAGTGC+TGG 0.574971 4:-83759221 MsG0480023327.01.T01:CDS
CAGACCATGCATGAAGGCTG+TGG 0.579264 4:+83759145 None:intergenic
GACCACCAATAATACTTCCA+AGG 0.580854 4:+83759684 None:intergenic
GATAACAGAACGATTGAACA+AGG 0.593019 4:+83760268 None:intergenic
TCCATCGTTTCAGATAATTG+TGG 0.595497 4:-83759438 MsG0480023327.01.T01:CDS
AAGAACCTGTAAGAAGGTGG+AGG 0.596913 4:+83760149 None:intergenic
GCAAATGACAAACCTGACCA+TGG 0.597618 4:+83759392 None:intergenic
AGAAATGCAGCAACTAGAGG+TGG 0.598755 4:-83758515 MsG0480023327.01.T01:CDS
GATTAGGTGGCAATTTCAAG+AGG 0.603130 4:-83759821 MsG0480023327.01.T01:intron
GAAGGCAGAGACGTTGACAT+TGG 0.604050 4:-83760418 MsG0480023327.01.T01:CDS
CAAATGACAAACCTGACCAT+GGG 0.604095 4:+83759393 None:intergenic
GGAGATGGCGACGAGGAATG+TGG 0.604110 4:+83760170 None:intergenic
TTTCAGATAATTGTGGCTCA+GGG 0.609333 4:-83759431 MsG0480023327.01.T01:CDS
CATTGATAGCAACCGTGGTG+TGG 0.609671 4:-83759735 MsG0480023327.01.T01:CDS
AAATGCAGCATCACTCGCCA+AGG 0.610382 4:-83758653 MsG0480023327.01.T01:CDS
CTGAATTGCTGGAATTACAA+TGG 0.612577 4:+83759778 None:intergenic
AGGCAACATTAACAGCAGAA+TGG 0.615504 4:+83759178 None:intergenic
CAAGGGATCGGAAAAGAGTG+AGG 0.615891 4:+83760286 None:intergenic
AATAAGAACCTGTAAGAAGG+TGG 0.616116 4:+83760146 None:intergenic
GCCAATAATCCAACAACAGG+AGG 0.618850 4:+83758748 None:intergenic
ATCAGAAATGCAGCAACTAG+AGG 0.627643 4:-83758518 MsG0480023327.01.T01:CDS
TAAAACCAGACCATGCATGA+AGG 0.628937 4:+83759139 None:intergenic
ACCACGGTTGCTATCAATGG+TGG 0.632248 4:+83759739 None:intergenic
AGAGACGTTGACATTGGTGC+TGG 0.637129 4:-83760412 MsG0480023327.01.T01:CDS
ACCGTGGTGTGGCATTTGGG+TGG 0.638640 4:-83759724 MsG0480023327.01.T01:CDS
AGAACGATTGAACAAGGGAT+CGG 0.640756 4:+83760274 None:intergenic
TCCCTACGGTTACCAAACAC+CGG 0.649237 4:-83759251 MsG0480023327.01.T01:CDS
TATCAGTTCTAACCAAAGAG+TGG 0.669602 4:+83759370 None:intergenic
TGATGATTTAGATTATCCAA+GGG 0.673834 4:-83758404 MsG0480023327.01.T01:CDS
AAGGGATCGGAAAAGAGTGA+GGG 0.678118 4:+83760287 None:intergenic
TCCACCATTGATAGCAACCG+TGG 0.681448 4:-83759740 MsG0480023327.01.T01:CDS
GTGGCGAGGTAAGCAGCCAG+AGG 0.682933 4:+83760246 None:intergenic
AGGTGGAGGAGATGGCGACG+AGG 0.683207 4:+83760163 None:intergenic
GAACTGATAGGCTTCTCACA+TGG 0.685795 4:-83759356 MsG0480023327.01.T01:CDS
GCCACCCAAATGCCACACCA+CGG 0.691633 4:+83759723 None:intergenic
TTGATCCAAAGCATATATAG+CGG 0.704808 4:+83758801 None:intergenic
ATGTGCACGATTGTGTCGCG+TGG 0.710569 4:+83760227 None:intergenic
ATAACAGAACGATTGAACAA+GGG 0.711522 4:+83760269 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTCAAAAATTAACTATT+TGG - Chr4:83759905-83759924 MsG0480023327.01.T01:intron 10.0%
!! ATATGCTAGAAAATTATAAA+AGG + Chr4:83759732-83759751 None:intergenic 15.0%
!!! TTTTTAGGATATATACAAAA+TGG + Chr4:83759789-83759808 None:intergenic 15.0%
!! ACATTATCAATCTATGTATT+TGG - Chr4:83758871-83758890 MsG0480023327.01.T01:intron 20.0%
!! GTTTGAATTACTATATAGTT+AGG + Chr4:83759836-83759855 None:intergenic 20.0%
!! TATATCCTAAAAATCGAAAA+TGG - Chr4:83759796-83759815 MsG0480023327.01.T01:CDS 20.0%
!! TTTAATAAAAGAAGCAAAAG+CGG - Chr4:83759300-83759319 MsG0480023327.01.T01:CDS 20.0%
!!! AGTTTTGCATAAATATGATT+AGG - Chr4:83758934-83758953 MsG0480023327.01.T01:intron 20.0%
!!! ATCTTTTGATTCAGAATAAA+AGG + Chr4:83759281-83759300 None:intergenic 20.0%
! AAACATTAATGACATGCATA+TGG + Chr4:83759887-83759906 None:intergenic 25.0%
! ATATGTTGAAACAAGTCAAA+AGG + Chr4:83758658-83758677 None:intergenic 25.0%
! ATGATGATTTAGATTATCCA+AGG - Chr4:83760366-83760385 MsG0480023327.01.T01:CDS 25.0%
! TATTTGGTAAGTATAGTGAA+TGG - Chr4:83758887-83758906 MsG0480023327.01.T01:intron 25.0%
! TGATGATTTAGATTATCCAA+GGG - Chr4:83760367-83760386 MsG0480023327.01.T01:CDS 25.0%
! TTATAGTTTCTGCTTCATTT+GGG - Chr4:83759464-83759483 MsG0480023327.01.T01:CDS 25.0%
! TTTATAGTTTCTGCTTCATT+TGG - Chr4:83759463-83759482 MsG0480023327.01.T01:CDS 25.0%
! TTTGCATAAATATGATTAGG+TGG - Chr4:83758937-83758956 MsG0480023327.01.T01:intron 25.0%
!! AGTTACCATTTTCGATTTTT+AGG + Chr4:83759804-83759823 None:intergenic 25.0%
!! GGTAGTACTAAATGAAATTT+TGG - Chr4:83758735-83758754 MsG0480023327.01.T01:CDS 25.0%
!! TAGTGTTATAGTTGGTATAT+TGG - Chr4:83759189-83759208 MsG0480023327.01.T01:CDS 25.0%
!! TTATTGGCACAAAATGTTTT+TGG - Chr4:83760038-83760057 MsG0480023327.01.T01:intron 25.0%
!!! TGATATCTGTTGTCATTTTT+TGG + Chr4:83759244-83759263 None:intergenic 25.0%
AAAAACAATACAGATTGCCT+TGG + Chr4:83760138-83760157 None:intergenic 30.0%
ACATCAATCTTTGGTATATC+TGG - Chr4:83759133-83759152 MsG0480023327.01.T01:CDS 30.0%
ACTCTATGTTTAATAGCTGT+TGG + Chr4:83759858-83759877 None:intergenic 30.0%
ATAACAGAACGATTGAACAA+GGG + Chr4:83758505-83758524 None:intergenic 30.0%
ATCGAAAATGGTAACTTCTT+AGG - Chr4:83759808-83759827 MsG0480023327.01.T01:CDS 30.0%
GCAATTTCAAGAGGTTTAAA+TGG - Chr4:83758959-83758978 MsG0480023327.01.T01:intron 30.0%
GTAAATAAGAACCTGTAAGA+AGG + Chr4:83758631-83758650 None:intergenic 30.0%
TATACCAAAGATTGATGTTG+AGG + Chr4:83759131-83759150 None:intergenic 30.0%
TCAACATATCTGTACTTGTT+TGG - Chr4:83758669-83758688 MsG0480023327.01.T01:CDS 30.0%
TCTTTACCATTCAGTTCATT+TGG + Chr4:83760332-83760351 None:intergenic 30.0%
TGAAGCAGAAACTATAAAGA+AGG + Chr4:83759461-83759480 None:intergenic 30.0%
TTAGAACCAAATGAACTGAA+TGG - Chr4:83760323-83760342 MsG0480023327.01.T01:CDS 30.0%
TTGATCCAAAGCATATATAG+CGG + Chr4:83759973-83759992 None:intergenic 30.0%
TTGATTCAGAATAAAAGGAC+TGG + Chr4:83759276-83759295 None:intergenic 30.0%
TTGTGACAACATTATTCTTC+CGG + Chr4:83759542-83759561 None:intergenic 30.0%
!! GCATTGATTAGTGTTATAGT+TGG - Chr4:83759181-83759200 MsG0480023327.01.T01:CDS 30.0%
!! GGAACTATTTCTGCAAATAT+TGG + Chr4:83759936-83759955 None:intergenic 30.0%
!!! TTGTCATTTTTTGGAAAGTG+TGG + Chr4:83759235-83759254 None:intergenic 30.0%
AACATTATTCTTCCGGTGTT+TGG + Chr4:83759535-83759554 None:intergenic 35.0%
AATAAGAACCTGTAAGAAGG+TGG + Chr4:83758628-83758647 None:intergenic 35.0%
ACAGTTAGTGTAGAAGTTGT+TGG - Chr4:83758784-83758803 MsG0480023327.01.T01:CDS 35.0%
ACTCTTTGGTTAGAACTGAT+AGG - Chr4:83759403-83759422 MsG0480023327.01.T01:CDS 35.0%
AGGAGCAAATGATTGCAATA+TGG + Chr4:83760006-83760025 None:intergenic 35.0%
ATGGTACAACTGCAATTCTT+TGG - Chr4:83759434-83759453 MsG0480023327.01.T01:CDS 35.0%
ATGTTGTCACAAATAAGTGC+TGG - Chr4:83759550-83759569 MsG0480023327.01.T01:CDS 35.0%
CAATCTTTGGTATATCTGGT+TGG - Chr4:83759137-83759156 MsG0480023327.01.T01:CDS 35.0%
CAGTTAGTGTAGAAGTTGTT+GGG - Chr4:83758785-83758804 MsG0480023327.01.T01:CDS 35.0%
CATTTAGTACTACCAAGTCT+AGG + Chr4:83758729-83758748 None:intergenic 35.0%
CCTATCTGTTTCGATTTCAA+CGG + Chr4:83760096-83760115 None:intergenic 35.0%
CGTTGAAATCGAAACAGATA+GGG - Chr4:83760094-83760113 MsG0480023327.01.T01:intron 35.0%
CTGAATTGCTGGAATTACAA+TGG + Chr4:83758996-83759015 None:intergenic 35.0%
GACTTGAACCAAAATTCATG+TGG + Chr4:83758695-83758714 None:intergenic 35.0%
GATAACAGAACGATTGAACA+AGG + Chr4:83758506-83758525 None:intergenic 35.0%
TCAAGAGGTTTAAATGGCAT+TGG - Chr4:83758965-83758984 MsG0480023327.01.T01:intron 35.0%
TCCATCGTTTCAGATAATTG+TGG - Chr4:83759333-83759352 MsG0480023327.01.T01:CDS 35.0%
TTGAAACAAGTCAAAAGGAG+AGG + Chr4:83758653-83758672 None:intergenic 35.0%
TTTCAGATAATTGTGGCTCA+GGG - Chr4:83759340-83759359 MsG0480023327.01.T01:CDS 35.0%
! AGTACATACTTGTTAGTTGC+CGG + Chr4:83759691-83759710 None:intergenic 35.0%
! TATCAGTTCTAACCAAAGAG+TGG + Chr4:83759404-83759423 None:intergenic 35.0%
! TTGTTTGGCCACATGAATTT+TGG - Chr4:83758684-83758703 MsG0480023327.01.T01:CDS 35.0%
!! AACCTTGGAAGTATTATTGG+TGG - Chr4:83759085-83759104 MsG0480023327.01.T01:intron 35.0%
!! GGAAACCTTGGAAGTATTAT+TGG - Chr4:83759082-83759101 MsG0480023327.01.T01:intron 35.0%
!!! CAACTTTTTTTGCCTAGACT+TGG - Chr4:83758714-83758733 MsG0480023327.01.T01:CDS 35.0%
AAATAGTTCCTGAGAAGTCC+CGG - Chr4:83759946-83759965 MsG0480023327.01.T01:intron 40.0%
AACTCTATGTTCTCTTCCCT+TGG + Chr4:83760386-83760405 None:intergenic 40.0%
AGAACGATTGAACAAGGGAT+CGG + Chr4:83758500-83758519 None:intergenic 40.0%
AGCAAGAATGGTTGCATTAG+AGG - Chr4:83760229-83760248 MsG0480023327.01.T01:CDS 40.0%
AGGCAACATTAACAGCAGAA+TGG + Chr4:83759596-83759615 None:intergenic 40.0%
ATCAGAAATGCAGCAACTAG+AGG - Chr4:83760253-83760272 MsG0480023327.01.T01:CDS 40.0%
CAAATGACAAACCTGACCAT+GGG + Chr4:83759381-83759400 None:intergenic 40.0%
CCAACTTCTTTGTATACTCC+TGG + Chr4:83758429-83758448 None:intergenic 40.0%
CCGTTGAAATCGAAACAGAT+AGG - Chr4:83760093-83760112 MsG0480023327.01.T01:intron 40.0%
CTACAACTAACAGGAAACCT+TGG - Chr4:83759070-83759089 MsG0480023327.01.T01:intron 40.0%
CTAGCCTCAACATCAATCTT+TGG - Chr4:83759124-83759143 MsG0480023327.01.T01:CDS 40.0%
GACCACCAATAATACTTCCA+AGG + Chr4:83759090-83759109 None:intergenic 40.0%
GATTAGGTGGCAATTTCAAG+AGG - Chr4:83758950-83758969 MsG0480023327.01.T01:intron 40.0%
GTTTCAGATAATTGTGGCTC+AGG - Chr4:83759339-83759358 MsG0480023327.01.T01:CDS 40.0%
TAAAACCAGACCATGCATGA+AGG + Chr4:83759635-83759654 None:intergenic 40.0%
TCAATCGTTCTGTTATCCTC+TGG - Chr4:83758509-83758528 MsG0480023327.01.T01:CDS 40.0%
TCCTCCTGTTGTTGGATTAT+TGG - Chr4:83760022-83760041 MsG0480023327.01.T01:intron 40.0%
TCGGAGATTACTTATCCCTA+CGG - Chr4:83759506-83759525 MsG0480023327.01.T01:CDS 40.0%
TGTGCCAATAATCCAACAAC+AGG + Chr4:83760029-83760048 None:intergenic 40.0%
! ACGGAATCAGAAGATCCTTT+TGG + Chr4:83760077-83760096 None:intergenic 40.0%
! ATGCAGACACAAAGTGTCAA+AGG + Chr4:83760167-83760186 None:intergenic 40.0%
! CATTCACTATTAGCTCTCAC+TGG + Chr4:83758851-83758870 None:intergenic 40.0%
! CCAGGAGTATACAAAGAAGT+TGG - Chr4:83758426-83758445 MsG0480023327.01.T01:CDS 40.0%
! GCCACAATTATCTGAAACGA+TGG + Chr4:83759337-83759356 None:intergenic 40.0%
! GTTTGTCATTTGCCACTCTT+TGG - Chr4:83759389-83759408 MsG0480023327.01.T01:CDS 40.0%
!! GAGTTTTTGGATCATTCCCA+TGG - Chr4:83759362-83759381 MsG0480023327.01.T01:CDS 40.0%
!! TGCTCTAGGTGCTTTTCTTT+GGG - Chr4:83758572-83758591 MsG0480023327.01.T01:CDS 40.0%
!! TTGCTCTAGGTGCTTTTCTT+TGG - Chr4:83758571-83758590 MsG0480023327.01.T01:CDS 40.0%
!!! GGTCTGGTTTTAGTCATCAT+GGG - Chr4:83759643-83759662 MsG0480023327.01.T01:CDS 40.0%
!!! TCAGAAGATCCTTTTGGAAC+TGG + Chr4:83760071-83760090 None:intergenic 40.0%
!!! TGGTCTGGTTTTAGTCATCA+TGG - Chr4:83759642-83759661 MsG0480023327.01.T01:CDS 40.0%
AACAGCAGAATGGCTGCTAA+AGG + Chr4:83759586-83759605 None:intergenic 45.0%
AAGAACCTGTAAGAAGGTGG+AGG + Chr4:83758625-83758644 None:intergenic 45.0%
AAGGGATCGGAAAAGAGTGA+GGG + Chr4:83758487-83758506 None:intergenic 45.0%
AGAAATGCAGCAACTAGAGG+TGG - Chr4:83760256-83760275 MsG0480023327.01.T01:CDS 45.0%
AGACAGAGATCGAGCAAGAA+TGG - Chr4:83760217-83760236 MsG0480023327.01.T01:CDS 45.0%
CAAAGCATATATAGCGGTCC+GGG + Chr4:83759967-83759986 None:intergenic 45.0%
CCAAAGCATATATAGCGGTC+CGG + Chr4:83759968-83759987 None:intergenic 45.0%
CGTGCACATGTTATTGCTCT+AGG - Chr4:83758558-83758577 MsG0480023327.01.T01:CDS 45.0%
GAACTGATAGGCTTCTCACA+TGG - Chr4:83759415-83759434 MsG0480023327.01.T01:CDS 45.0%
GCAAATGACAAACCTGACCA+TGG + Chr4:83759382-83759401 None:intergenic 45.0%
GCAACTAGAGACTGAATTGC+TGG + Chr4:83759007-83759026 None:intergenic 45.0%
GCCAATAATCCAACAACAGG+AGG + Chr4:83760026-83760045 None:intergenic 45.0%
GCTTCATTTGGGTCTCTGTT+TGG - Chr4:83759475-83759494 MsG0480023327.01.T01:CDS 45.0%
GGCTATAGACCAGTTCCAAA+AGG - Chr4:83760059-83760078 MsG0480023327.01.T01:intron 45.0%
TCATTTGCTCCTCCTGTTGT+TGG - Chr4:83760014-83760033 MsG0480023327.01.T01:intron 45.0%
TCATTTGGGTCTCTGTTTGG+AGG - Chr4:83759478-83759497 MsG0480023327.01.T01:CDS 45.0%
TCTCTGTTTGGAGGATGGTT+CGG - Chr4:83759487-83759506 MsG0480023327.01.T01:CDS 45.0%
TTCTACACTAACTGTCCCCA+TGG + Chr4:83758779-83758798 None:intergenic 45.0%
TTTGGATCATTCCCATGGTC+AGG - Chr4:83759367-83759386 MsG0480023327.01.T01:CDS 45.0%
TTTGGGTGGCTACAACTAAC+AGG - Chr4:83759061-83759080 MsG0480023327.01.T01:intron 45.0%
! ATTGTGGCTCAGGGAGTTTT+TGG - Chr4:83759349-83759368 MsG0480023327.01.T01:CDS 45.0%
!! TCATGGGATTTGCTACATCC+TGG - Chr4:83759659-83759678 MsG0480023327.01.T01:CDS 45.0%
AAATGCAGCATCACTCGCCA+AGG - Chr4:83760118-83760137 MsG0480023327.01.T01:intron 50.0%
AACGCTGATCCAACTGCGTT+AGG - Chr4:83758459-83758478 MsG0480023327.01.T01:CDS 50.0%
AAGGCTGTGGATGGATCATC+AGG + Chr4:83759616-83759635 None:intergenic 50.0%
ACCACGGTTGCTATCAATGG+TGG + Chr4:83759035-83759054 None:intergenic 50.0%
AGAGACGTTGACATTGGTGC+TGG - Chr4:83758359-83758378 MsG0480023327.01.T01:CDS 50.0%
CAAGGGATCGGAAAAGAGTG+AGG + Chr4:83758488-83758507 None:intergenic 50.0%
CACACCACGGTTGCTATCAA+TGG + Chr4:83759038-83759057 None:intergenic 50.0%
CATCTCCTCCACCTTCTTAC+AGG - Chr4:83758617-83758636 MsG0480023327.01.T01:CDS 50.0%
CCGGACCGCTATATATGCTT+TGG - Chr4:83759965-83759984 MsG0480023327.01.T01:intron 50.0%
CTCTCGGGTATGTGCAGTAA+AGG + Chr4:83760199-83760218 None:intergenic 50.0%
CTGTAAGAAGGTGGAGGAGA+TGG + Chr4:83758619-83758638 None:intergenic 50.0%
CTTGCTCGATCTCTGTCTCT+CGG + Chr4:83760215-83760234 None:intergenic 50.0%
GAAGGCAGAGACGTTGACAT+TGG - Chr4:83758353-83758372 MsG0480023327.01.T01:CDS 50.0%
TCCCTACGGTTACCAAACAC+CGG - Chr4:83759520-83759539 MsG0480023327.01.T01:CDS 50.0%
TGCTACATCCTGGAATGCTC+CGG - Chr4:83759669-83759688 MsG0480023327.01.T01:CDS 50.0%
TTGCTCGATCTCTGTCTCTC+GGG + Chr4:83760214-83760233 None:intergenic 50.0%
TTGGGTCTCTGTTTGGAGGA+TGG - Chr4:83759482-83759501 MsG0480023327.01.T01:CDS 50.0%
! CATTGATAGCAACCGTGGTG+TGG - Chr4:83759036-83759055 MsG0480023327.01.T01:intron 50.0%
! TCCACCATTGATAGCAACCG+TGG - Chr4:83759031-83759050 MsG0480023327.01.T01:intron 50.0%
! TCCGGTGTTTGGTAACCGTA+GGG + Chr4:83759524-83759543 None:intergenic 50.0%
! TTCCGGTGTTTGGTAACCGT+AGG + Chr4:83759525-83759544 None:intergenic 50.0%
AACTGTCCCCATGGACAGCT+GGG + Chr4:83758770-83758789 None:intergenic 55.0%
AGCAACCGTGGTGTGGCATT+TGG - Chr4:83759043-83759062 MsG0480023327.01.T01:intron 55.0%
ATGTGCACGATTGTGTCGCG+TGG + Chr4:83758547-83758566 None:intergenic 55.0%
CACAGCCTTCATGCATGGTC+TGG - Chr4:83759627-83759646 MsG0480023327.01.T01:CDS 55.0%
CAGACCATGCATGAAGGCTG+TGG + Chr4:83759629-83759648 None:intergenic 55.0%
CCATCCACAGCCTTCATGCA+TGG - Chr4:83759622-83759641 MsG0480023327.01.T01:CDS 55.0%
CCATGCATGAAGGCTGTGGA+TGG + Chr4:83759625-83759644 None:intergenic 55.0%
GCAACCGTGGTGTGGCATTT+GGG - Chr4:83759044-83759063 MsG0480023327.01.T01:intron 55.0%
GCTGATGAGTCATTGCTGCC+AGG - Chr4:83758408-83758427 MsG0480023327.01.T01:CDS 55.0%
GTGAGGGAACCTAACGCAGT+TGG + Chr4:83758471-83758490 None:intergenic 55.0%
TAACTGTCCCCATGGACAGC+TGG + Chr4:83758771-83758790 None:intergenic 55.0%
! GTTAGTTGCCGGAGCATTCC+AGG + Chr4:83759680-83759699 None:intergenic 55.0%
ACCGTGGTGTGGCATTTGGG+TGG - Chr4:83759047-83759066 MsG0480023327.01.T01:intron 60.0%
ATAGCGGTCCGGGACTTCTC+AGG + Chr4:83759957-83759976 None:intergenic 60.0%
GCCACCCAAATGCCACACCA+CGG + Chr4:83759051-83759070 None:intergenic 60.0%
GGAGATGGCGACGAGGAATG+TGG + Chr4:83758604-83758623 None:intergenic 60.0%
AGGTGGAGGAGATGGCGACG+AGG + Chr4:83758611-83758630 None:intergenic 65.0%
CACGATTGTGTCGCGTGGCG+AGG + Chr4:83758542-83758561 None:intergenic 65.0%
CCATGGACAGCTGGGACAGG+GGG + Chr4:83758762-83758781 None:intergenic 65.0%
CCCATGGACAGCTGGGACAG+GGG + Chr4:83758763-83758782 None:intergenic 65.0%
CCCCATGGACAGCTGGGACA+GGG + Chr4:83758764-83758783 None:intergenic 65.0%
CCCCTGTCCCAGCTGTCCAT+GGG - Chr4:83758760-83758779 MsG0480023327.01.T01:CDS 65.0%
CCCTGTCCCAGCTGTCCATG+GGG - Chr4:83758761-83758780 MsG0480023327.01.T01:CDS 65.0%
GTGGCGAGGTAAGCAGCCAG+AGG + Chr4:83758528-83758547 None:intergenic 65.0%
TCCCCATGGACAGCTGGGAC+AGG + Chr4:83758765-83758784 None:intergenic 65.0%
CCCCCTGTCCCAGCTGTCCA+TGG - Chr4:83758759-83758778 MsG0480023327.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 83758351 83760442 83758351 ID=MsG0480023327.01;Name=MsG0480023327.01
Chr4 mRNA 83758351 83760442 83758351 ID=MsG0480023327.01.T01;Parent=MsG0480023327.01;Name=MsG0480023327.01.T01;_AED=0.48;_eAED=0.48;_QI=0|0|0|1|1|1|3|0|514
Chr4 exon 83760155 83760442 83760155 ID=MsG0480023327.01.T01:exon:5589;Parent=MsG0480023327.01.T01
Chr4 exon 83759092 83759837 83759092 ID=MsG0480023327.01.T01:exon:5588;Parent=MsG0480023327.01.T01
Chr4 exon 83758351 83758861 83758351 ID=MsG0480023327.01.T01:exon:5587;Parent=MsG0480023327.01.T01
Chr4 CDS 83760155 83760442 83760155 ID=MsG0480023327.01.T01:cds;Parent=MsG0480023327.01.T01
Chr4 CDS 83759092 83759837 83759092 ID=MsG0480023327.01.T01:cds;Parent=MsG0480023327.01.T01
Chr4 CDS 83758351 83758861 83758351 ID=MsG0480023327.01.T01:cds;Parent=MsG0480023327.01.T01
Gene Sequence

>MsG0480023327.01.T01

ATGAAGGCAGAGACGTTGACATTGGTGCTGGTGAATCTAGCAAGTATTATGCAGAGAGCTGATGAGTCATTGCTGCCAGGAGTATACAAAGAAGTTGGTGCTGAACTTAACGCTGATCCAACTGCGTTAGGTTCCCTCACTCTTTTCCGATCCCTTGTTCAATCGTTCTGTTATCCTCTGGCTGCTTACCTCGCCACGCGACACAATCGTGCACATGTTATTGCTCTAGGTGCTTTTCTTTGGGCTGCTGCCACATTCCTCGTCGCCATCTCCTCCACCTTCTTACAGGTGGCAATTTCAAGAGGTTTAAATGGCATTGGACTAGCCATTGTAATTCCAGCAATTCAGTCTCTAGTTGCTGACTCCACCATTGATAGCAACCGTGGTGTGGCATTTGGGTGGCTACAACTAACAGGAAACCTTGGAAGTATTATTGGTGGTCTTTTCTCAGTACTACTAGCCTCAACATCAATCTTTGGTATATCTGGTTGGAGAATAGCTTTTCATCTTGTTGCATTGATTAGTGTTATAGTTGGTATATTGGTACGCATCTACGCTAATGATCCACACTTTCCAAAAAATGACAACAGATATCAAACTTCAAACCAGTCCTTTTATTCTGAATCAAAAGATTTAATAAAAGAAGCAAAAGCGGTTATAAAAATTCCATCGTTTCAGATAATTGTGGCTCAGGGAGTTTTTGGATCATTCCCATGGTCAGGTTTGTCATTTGCCACTCTTTGGTTAGAACTGATAGGCTTCTCACATGGTACAACTGCAATTCTTTGGACCTTCTTTATAGTTTCTGCTTCATTTGGGTCTCTGTTTGGAGGATGGTTCGGAGATTACTTATCCCTACGGTTACCAAACACCGGAAGAATAATGTTGTCACAAATAAGTGCTGGTTCAGCTGTTCCTTTAGCAGCCATTCTGCTGTTAATGTTGCCTGATGATCCATCCACAGCCTTCATGCATGGTCTGGTTTTAGTCATCATGGGATTTGCTACATCCTGGAATGCTCCGGCAACTAACAATCCAATATTTGCAGAAATAGTTCCTGAGAAGTCCCGGACCGCTATATATGCTTTGGATCAATCTTTCGAGTCCATATTGCAATCATTTGCTCCTCCTGTTGTTGGATTATTGGCACAAAATGTTTTTGGCTATAGACCAGTTCCAAAAGGATCTTCTGATTCCGTTGAAATCGAAACAGATAGGGAAAATGCAGCATCACTCGCCAAGGCAATCTGTATTGTTTTTGTAATTCCTTTGACACTTTGTGTCTGCATCTACTCATTCCTTTACTGCACATACCCGAGAGACAGAGATCGAGCAAGAATGGTTGCATTAGAGGAATCAGAAATGCAGCAACTAGAGGTGGAAGATTGCACAAGAGAAAAAGAAGAATATTGTGAAATTCATGTTTTAGAACCAAATGAACTGAATGGTAAAGAAAGTAGCAAGACTGATGATGATTTAGATTATCCAAGGGAAGAGAACATAGAGTTAGATGATAATGATGAGAAAGTTTTGTTGTCCCGATAG
Protein sequence

>MsG0480023327.01.T01

MKAETLTLVLVNLASIMQRADESLLPGVYKEVGAELNADPTALGSLTLFRSLVQSFCYPLAAYLATRHNRAHVIALGAFLWAAATFLVAISSTFLQVAISRGLNGIGLAIVIPAIQSLVADSTIDSNRGVAFGWLQLTGNLGSIIGGLFSVLLASTSIFGISGWRIAFHLVALISVIVGILVRIYANDPHFPKNDNRYQTSNQSFYSESKDLIKEAKAVIKIPSFQIIVAQGVFGSFPWSGLSFATLWLELIGFSHGTTAILWTFFIVSASFGSLFGGWFGDYLSLRLPNTGRIMLSQISAGSAVPLAAILLLMLPDDPSTAFMHGLVLVIMGFATSWNAPATNNPIFAEIVPEKSRTAIYALDQSFESILQSFAPPVVGLLAQNVFGYRPVPKGSSDSVEIETDRENAASLAKAICIVFVIPLTLCVCIYSFLYCTYPRDRDRARMVALEESEMQQLEVEDCTREKEEYCEIHVLEPNELNGKESSKTDDDLDYPREENIELDDNDEKVLLSR*