AlfalfaGEDB | MsG0480023609.01

M. sativa cultivar ZhongmuNo.1 / MsG0480023609.01

KEGG: -
GO: GO:0003677 GO:0005634
PFAM: PF01429

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023609.01.T01	XP_013447003.1	81.221	213	16	1	56	244	169	381	7.39E-106	343
MsG0480023609.01.T01	XP_013447003.1	57.724	123	20	1	1	123	1	91	1.46E-31	134

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023609.01.T01	A0A072U5A2	81.221	213	16	1	56	244	169	381	3.53e-106	343

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048374.01	MsG0480023609.01	0.820418	6.932566e-53	5.482634e-50
MsG0080048602.01	MsG0480023609.01	0.856179	3.832495e-62	9.067589e-59
MsG0180004383.01	MsG0480023609.01	0.831817	1.346103e-55	1.477441e-52
MsG0180004872.01	MsG0480023609.01	0.832134	1.123950e-55	1.244942e-52
MsG0180005246.01	MsG0480023609.01	0.854991	8.509688e-62	1.935625e-58
MsG0280006304.01	MsG0480023609.01	0.832778	7.783896e-56	8.788911e-53
MsG0280007726.01	MsG0480023609.01	0.825719	4.021422e-54	3.694977e-51
MsG0280007830.01	MsG0480023609.01	0.813498	2.482551e-51	1.623936e-48
MsG0280010563.01	MsG0480023609.01	0.861195	1.216491e-63	3.413337e-60
MsG0280010932.01	MsG0480023609.01	0.820213	7.726056e-53	6.075135e-50

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023609.01.T01	MTR_8g096930	81.221	213	16	1	56	244	169	381	8.95e-110	343
MsG0480023609.01.T01	MTR_8g096930	57.724	123	20	1	1	123	1	91	1.77e-35	134

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023609.01.T01	AT5G52230	38.298	94	51	3	45	136	16	104	1.95e-11	63.9
MsG0480023609.01.T01	AT5G52230	38.298	94	51	3	45	136	16	104	1.95e-11	63.9
MsG0480023609.01.T01	AT5G52230	38.298	94	51	3	45	136	16	104	1.95e-11	63.9

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Find 82 sgRNAs with CRISPR-Local

Find 342 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACGTTGTACATCCTTCT+TGG	0.276092	4:+87601653	None:intergenic
AAATTAAACGTGTCAGTGTC+GGG	0.467095	4:-87604217	MsG0480023609.01.T01:five_prime_UTR
AAGATTCGGAAGCACAGAAG+TGG	0.628849	4:-87601940	MsG0480023609.01.T01:CDS
AAGCTGACGAACGTCCTGAA+TGG	0.559865	4:-87603841	MsG0480023609.01.T01:CDS
AAGTGGCATAAAGAAAGATT+GGG	0.512803	4:-87601923	MsG0480023609.01.T01:intron
AATCTGGAGGAACCAACAAA+CGG	0.560339	4:-87604014	MsG0480023609.01.T01:CDS
ACATGTGTGAGTGTCAGTTG+CGG	0.703758	4:+87604267	None:intergenic
ACATTGTCAACCAACTTGAC+AGG	0.528995	4:+87599343	None:intergenic
ACCAGTTGAAAGCTTAGAAT+TGG	0.381405	4:-87599536	MsG0480023609.01.T01:CDS
ACGAAATGGATAATAATTGA+AGG	0.522574	4:+87604877	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACAATGGAGAGAGAAAA+AGG	+	Chr4:87599790-87599809	None:intergenic	30.0%
AAAAGCAGCAATAATGAGAT+AGG	+	Chr4:87602681-87602700	None:intergenic	30.0%
AAACATGAAAAATAAAGCGG+GGG	-	Chr4:87603105-87603124	MsG0480023609.01.T01:intron	30.0%
AAATATGTAACAGAAGGATG+GGG	+	Chr4:87603872-87603891	None:intergenic	30.0%
AAGTGGCATAAAGAAAGATT+GGG	-	Chr4:87602291-87602310	MsG0480023609.01.T01:intron	30.0%
AAGTTGAAATACAAAGAAGC+GGG	+	Chr4:87604641-87604660	None:intergenic	30.0%
AATACAAACTAATGTGAGCA+TGG	+	Chr4:87600877-87600896	None:intergenic	30.0%
ACAAATATGTAACAGAAGGA+TGG	+	Chr4:87603874-87603893	None:intergenic	30.0%
ACGACATCTTGAAACTATAA+AGG	-	Chr4:87601252-87601271	MsG0480023609.01.T01:intron	30.0%
ACTTTGCATGTAGACTTAAA+AGG	+	Chr4:87600635-87600654	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	87599303	87604933	87599303	ID=MsG0480023609.01;Name=MsG0480023609.01
Chr4	mRNA	87599303	87604933	87599303	ID=MsG0480023609.01.T01;Parent=MsG0480023609.01;Name=MsG0480023609.01.T01;_AED=0.37;_eAED=0.37;_QI=682\|0.85\|1\|1\|0.71\|0.5\|8\|0\|246
Chr4	exon	87599303	87599565	87599303	ID=MsG0480023609.01.T01:exon:1886;Parent=MsG0480023609.01.T01
Chr4	exon	87599691	87599754	87599691	ID=MsG0480023609.01.T01:exon:1885;Parent=MsG0480023609.01.T01
Chr4	exon	87601578	87601712	87601578	ID=MsG0480023609.01.T01:exon:1884;Parent=MsG0480023609.01.T01
Chr4	exon	87601924	87602019	87601924	ID=MsG0480023609.01.T01:exon:1883;Parent=MsG0480023609.01.T01
Chr4	exon	87603772	87603873	87603772	ID=MsG0480023609.01.T01:exon:1882;Parent=MsG0480023609.01.T01
Chr4	exon	87603978	87604347	87603978	ID=MsG0480023609.01.T01:exon:1881;Parent=MsG0480023609.01.T01
Chr4	exon	87604541	87604933	87604541	ID=MsG0480023609.01.T01:exon:1880;Parent=MsG0480023609.01.T01
Chr4	five_prime_UTR	87604541	87604933	87604541	ID=MsG0480023609.01.T01:five_prime_utr;Parent=MsG0480023609.01.T01
Chr4	five_prime_UTR	87604059	87604347	87604059	ID=MsG0480023609.01.T01:five_prime_utr;Parent=MsG0480023609.01.T01
Chr4	CDS	87603978	87604058	87603978	ID=MsG0480023609.01.T01:cds;Parent=MsG0480023609.01.T01
Chr4	CDS	87603772	87603873	87603772	ID=MsG0480023609.01.T01:cds;Parent=MsG0480023609.01.T01
Chr4	CDS	87601924	87602019	87601924	ID=MsG0480023609.01.T01:cds;Parent=MsG0480023609.01.T01
Chr4	CDS	87601578	87601712	87601578	ID=MsG0480023609.01.T01:cds;Parent=MsG0480023609.01.T01
Chr4	CDS	87599691	87599754	87599691	ID=MsG0480023609.01.T01:cds;Parent=MsG0480023609.01.T01
Chr4	CDS	87599303	87599565	87599303	ID=MsG0480023609.01.T01:cds;Parent=MsG0480023609.01.T01

Gene Sequence

>MsG0480023609.01.T01

ATGGGAAAACCAAACGTTAAAAAATCTGGAGGAACCAACAAACGGATTGACAATGACACAAAACGCAACTCTAAACAACAAACTCCGGAGAAAGCTGACGAACGTCCTGAATGGTTGCCTAATGGCTGGGATATCGAAGTTAGAACCCGACAAAGTGGTCCGGCTATGGGATCTGCATATAAGGTTGCAGGCGAGAATTCCATTGTGGAGGATCTACCACCTGGATGGATTAAAGAAGTAAAGATTCGGAAGCACAGAAGTGGCATAAAGAAAGATTGGTTTTATATTGATCCAGTGAGTGGATATGTATTCCATTCCAAGAAGGATGTACAACGTTTTCTCAAATCTGGAGATATAGCCAAGTGTGCCATTAAACCAAGTAGAAGACAATATCAAGATGAAGACAACTCAACTCCATCACCTGCTGCCAAGAGACAAAAACCAAAGCAGTCTACACCCATGCAAAAGATTTTTGTTGCCAAAGAACCAGTTGAAAGCTTAGAATTGGCCGATGTTAACAACTCAAGGAAAGGGCAAGATGTGAATGTGCCATCTCGAATGATGGTTGCTCTTGATACCTCAGGGGAATCCTTGAAAATGAATCACAGTAACCATAGCATATCCAAAAATGATGTCAATGTGAGCCATCGGTTTTCACGGAGACTGGCTGGTGTTGAACCTGTCAAGTTGGTTGACAATGTGATTAATGATGAGGCTCTTCTAGTTCCAAAAGAAACTTGA

Protein sequence

>MsG0480023609.01.T01

MGKPNVKKSGGTNKRIDNDTKRNSKQQTPEKADERPEWLPNGWDIEVRTRQSGPAMGSAYKVAGENSIVEDLPPGWIKEVKIRKHRSGIKKDWFYIDPVSGYVFHSKKDVQRFLKSGDIAKCAIKPSRRQYQDEDNSTPSPAAKRQKPKQSTPMQKIFVAKEPVESLELADVNNSRKGQDVNVPSRMMVALDTSGESLKMNHSNHSISKNDVNVSHRFSRRLAGVEPVKLVDNVINDEALLVPKET*