Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006304.01.T01 | XP_003626539.1 | 82.805 | 442 | 35 | 5 | 1 | 405 | 1 | 438 | 0 | 699 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006304.01.T01 | Q9XHM1 | 81.9 | 442 | 38 | 6 | 1 | 405 | 1 | 437 | 0 | 679 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006304.01.T01 | G7L407 | 82.805 | 442 | 35 | 5 | 1 | 405 | 1 | 438 | 0.0 | 699 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047793.01 | MsG0280006304.01 | 0.811597 | 6.466806e-51 | 4.019588e-48 |
| MsG0080048374.01 | MsG0280006304.01 | 0.933156 | 2.883441e-95 | 2.016503e-90 |
| MsG0080048602.01 | MsG0280006304.01 | 0.822031 | 2.943876e-53 | 2.435449e-50 |
| MsG0180000146.01 | MsG0280006304.01 | 0.822512 | 2.276492e-53 | 1.909545e-50 |
| MsG0180003303.01 | MsG0280006304.01 | 0.805677 | 1.189251e-49 | 6.330644e-47 |
| MsG0180003448.01 | MsG0280006304.01 | 0.833852 | 4.202023e-56 | 4.900143e-53 |
| MsG0180003799.01 | MsG0280006304.01 | 0.821611 | 3.683558e-53 | 3.011194e-50 |
| MsG0180003928.01 | MsG0280006304.01 | 0.834364 | 3.128602e-56 | 3.704655e-53 |
| MsG0180003937.01 | MsG0280006304.01 | 0.826560 | 2.536805e-54 | 2.387280e-51 |
| MsG0180004014.01 | MsG0280006304.01 | 0.806990 | 6.290554e-50 | 3.463074e-47 |
| MsG0180004383.01 | MsG0280006304.01 | 0.869252 | 3.558888e-66 | 1.326624e-62 |
| MsG0180005026.01 | MsG0280006304.01 | 0.801929 | 7.140042e-49 | 3.452438e-46 |
| MsG0180005504.01 | MsG0280006304.01 | 0.818177 | 2.245583e-52 | 1.668854e-49 |
| MsG0180005985.01 | MsG0280006304.01 | 0.843908 | 1.048883e-58 | 1.665641e-55 |
| MsG0280006304.01 | MsG0280006351.01 | 0.827767 | 1.304477e-54 | 1.271384e-51 |
| MsG0280006304.01 | MsG0280007352.01 | 0.800108 | 1.682576e-48 | 7.768996e-46 |
| MsG0280006304.01 | MsG0280007726.01 | 0.823818 | 1.128379e-53 | 9.821457e-51 |
| MsG0280006304.01 | MsG0280007803.01 | 0.825218 | 5.285501e-54 | 4.787723e-51 |
| MsG0280006304.01 | MsG0280007829.01 | 0.807110 | 5.930761e-50 | 3.275162e-47 |
| MsG0280006304.01 | MsG0280007830.01 | 0.824297 | 8.712211e-54 | 7.685142e-51 |
| MsG0280006304.01 | MsG0280008574.01 | 0.828846 | 7.163533e-55 | 7.204434e-52 |
| MsG0280006304.01 | MsG0280009386.01 | 0.833216 | 6.056165e-56 | 6.928554e-53 |
| MsG0280006304.01 | MsG0280009462.01 | 0.811072 | 8.404299e-51 | 5.152402e-48 |
| MsG0280006304.01 | MsG0280010563.01 | 0.871695 | 5.616292e-67 | 2.286759e-63 |
| MsG0280006304.01 | MsG0280010735.01 | 0.800205 | 1.607692e-48 | 7.441532e-46 |
| MsG0280006304.01 | MsG0280010866.01 | 0.836213 | 1.067743e-56 | 1.336736e-53 |
| MsG0280006304.01 | MsG0280010932.01 | 0.804651 | 1.950429e-49 | 1.011177e-46 |
| MsG0280006304.01 | MsG0280011075.01 | 0.827059 | 1.928624e-54 | 1.841342e-51 |
| MsG0280006304.01 | MsG0280011085.01 | 0.829184 | 5.934025e-55 | 6.028614e-52 |
| MsG0280006304.01 | MsG0380013069.01 | 0.813099 | 3.037963e-51 | 1.965546e-48 |
| MsG0280006304.01 | MsG0380015495.01 | 0.850336 | 1.813496e-60 | 3.540354e-57 |
| MsG0280006304.01 | MsG0380016034.01 | 0.801581 | 8.414863e-49 | 4.033067e-46 |
| MsG0280006304.01 | MsG0380016589.01 | 0.896760 | 2.707479e-76 | 2.969506e-72 |
| MsG0280006304.01 | MsG0380016850.01 | 0.805285 | 1.436855e-49 | 7.571816e-47 |
| MsG0280006304.01 | MsG0380016871.01 | 0.836423 | 9.442320e-57 | 1.189399e-53 |
| MsG0280006304.01 | MsG0380016872.01 | 0.814181 | 1.755852e-51 | 1.169970e-48 |
| MsG0280006304.01 | MsG0380016927.01 | 0.827486 | 1.523613e-54 | 1.472972e-51 |
| MsG0280006304.01 | MsG0380017045.01 | 0.801599 | 8.345099e-49 | 4.001297e-46 |
| MsG0280006304.01 | MsG0380017374.01 | 0.858720 | 6.785000e-63 | 1.748255e-59 |
| MsG0280006304.01 | MsG0480018256.01 | 0.823303 | 1.489273e-53 | 1.277396e-50 |
| MsG0280006304.01 | MsG0480018643.01 | 0.856650 | 2.787540e-62 | 6.699543e-59 |
| MsG0280006304.01 | MsG0480019395.01 | 0.832990 | 6.896301e-56 | 7.836642e-53 |
| MsG0280006304.01 | MsG0480020047.01 | 0.804172 | 2.453989e-49 | 1.256654e-46 |
| MsG0280006304.01 | MsG0480021216.01 | 0.856971 | 2.242171e-62 | 5.447141e-59 |
| MsG0280006304.01 | MsG0480021914.01 | 0.829296 | 5.573810e-55 | 5.682000e-52 |
| MsG0280006304.01 | MsG0480022317.01 | 0.909686 | 4.328518e-82 | 8.521503e-78 |
| MsG0280006304.01 | MsG0480022398.01 | 0.881114 | 3.141689e-70 | 1.817306e-66 |
| MsG0280006304.01 | MsG0480022719.01 | 0.838140 | 3.432664e-57 | 4.555617e-54 |
| MsG0280006304.01 | MsG0480022819.01 | 0.833355 | 5.593427e-56 | 6.425825e-53 |
| MsG0280006304.01 | MsG0480023609.01 | 0.832778 | 7.783896e-56 | 8.788911e-53 |
| MsG0280006304.01 | MsG0480023697.01 | 0.830140 | 3.473526e-55 | 3.628215e-52 |
| MsG0280006304.01 | MsG0580024612.01 | 0.821078 | 4.888646e-53 | 3.937846e-50 |
| MsG0280006304.01 | MsG0580024770.01 | 0.827496 | 1.515156e-54 | 1.465213e-51 |
| MsG0280006304.01 | MsG0580024959.01 | 0.852644 | 4.033580e-61 | 8.484103e-58 |
| MsG0280006304.01 | MsG0580025013.01 | 0.820521 | 6.564399e-53 | 5.205743e-50 |
| MsG0280006304.01 | MsG0580025128.01 | 0.884349 | 2.068752e-71 | 1.360333e-67 |
| MsG0280006304.01 | MsG0580025730.01 | 0.802901 | 4.501757e-49 | 2.231390e-46 |
| MsG0280006304.01 | MsG0580028472.01 | 0.849138 | 3.918142e-60 | 7.357630e-57 |
| MsG0280006304.01 | MsG0580029007.01 | 0.835325 | 1.792289e-56 | 2.184525e-53 |
| MsG0280006304.01 | MsG0580029036.01 | 0.828446 | 8.952211e-55 | 8.900425e-52 |
| MsG0280006304.01 | MsG0580029214.01 | 0.865476 | 5.744079e-65 | 1.871377e-61 |
| MsG0280006304.01 | MsG0580029540.01 | 0.843559 | 1.300626e-58 | 2.042771e-55 |
| MsG0280006304.01 | MsG0580030100.01 | 0.800715 | 1.265553e-48 | 5.934036e-46 |
| MsG0280006304.01 | MsG0680035681.01 | 0.815219 | 1.034254e-51 | 7.087383e-49 |
| MsG0280006304.01 | MsG0680035874.01 | 0.810507 | 1.113659e-50 | 6.725308e-48 |
| MsG0280006304.01 | MsG0780035936.01 | 0.839623 | 1.418246e-57 | 1.969681e-54 |
| MsG0280006304.01 | MsG0780037301.01 | 0.864018 | 1.644386e-64 | 5.086953e-61 |
| MsG0280006304.01 | MsG0780037876.01 | 0.830618 | 2.653272e-55 | 2.810211e-52 |
| MsG0280006304.01 | MsG0780037920.01 | 0.832123 | 1.131050e-55 | 1.252408e-52 |
| MsG0280006304.01 | MsG0780039497.01 | 0.872310 | 3.508755e-67 | 1.461163e-63 |
| MsG0280006304.01 | MsG0780039530.01 | 0.920741 | 8.711079e-88 | 3.002449e-83 |
| MsG0280006304.01 | MsG0780040027.01 | 0.809784 | 1.594323e-50 | 9.447537e-48 |
| MsG0280006304.01 | MsG0780040049.01 | 0.841713 | 4.022009e-58 | 5.959942e-55 |
| MsG0280006304.01 | MsG0780040058.01 | 0.828396 | 9.199942e-55 | 9.133766e-52 |
| MsG0280006304.01 | MsG0780040136.01 | 0.819938 | 8.929114e-53 | 6.967985e-50 |
| MsG0280006304.01 | MsG0780040687.01 | 0.827915 | 1.201655e-54 | 1.176307e-51 |
| MsG0280006304.01 | MsG0780040761.01 | 0.831582 | 1.538686e-55 | 1.677011e-52 |
| MsG0280006304.01 | MsG0780041371.01 | 0.810388 | 1.181803e-50 | 7.113941e-48 |
| MsG0280006304.01 | MsG0780041598.01 | 0.842087 | 3.202974e-58 | 4.803150e-55 |
| MsG0280006304.01 | MsG0880041982.01 | 0.814010 | 1.915523e-51 | 1.270455e-48 |
| MsG0280006304.01 | MsG0880042838.01 | 0.867532 | 1.277293e-65 | 4.474245e-62 |
| MsG0280006304.01 | MsG0880042983.01 | 0.805591 | 1.239508e-49 | 6.583520e-47 |
| MsG0280006304.01 | MsG0880045505.01 | 0.805743 | 1.152183e-49 | 6.143915e-47 |
| MsG0280006304.01 | MsG0880045841.01 | 0.877024 | 8.752311e-69 | 4.334726e-65 |
| MsG0280006304.01 | MsG0880046100.01 | 0.850113 | 2.095045e-60 | 4.060153e-57 |
| MsG0280006304.01 | MsG0880046893.01 | 0.843740 | 1.163189e-58 | 1.837264e-55 |
| MsG0280006304.01 | MsG0880047289.01 | 0.805986 | 1.024316e-49 | 5.496181e-47 |
| MsG0280006304.01 | MsG0880047293.01 | 0.813778 | 2.154596e-51 | 1.420028e-48 |
| MsG0280006304.01 | MsG0880047457.01 | 0.810151 | 1.329442e-50 | 7.953344e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006304.01.T01 | MTR_7g116960 | 82.805 | 442 | 35 | 5 | 1 | 405 | 1 | 438 | 0.0 | 699 |
| MsG0280006304.01.T01 | MTR_2g005610 | 83.371 | 445 | 26 | 5 | 1 | 405 | 50 | 486 | 0.0 | 687 |
| MsG0280006304.01.T01 | MTR_1g023190 | 81.674 | 442 | 39 | 6 | 1 | 405 | 1 | 437 | 0.0 | 676 |
| MsG0280006304.01.T01 | MTR_6g046050 | 46.082 | 319 | 88 | 7 | 89 | 405 | 4 | 240 | 3.84e-72 | 234 |
| MsG0280006304.01.T01 | MTR_6g046630 | 63.536 | 181 | 37 | 4 | 226 | 404 | 62 | 215 | 3.09e-62 | 210 |
| MsG0280006304.01.T01 | MTR_2g078570 | 53.403 | 191 | 19 | 4 | 217 | 406 | 45 | 166 | 3.24e-53 | 177 |
| MsG0280006304.01.T01 | MTR_6g045905 | 54.286 | 105 | 29 | 4 | 217 | 321 | 52 | 137 | 6.50e-24 | 97.1 |
| MsG0280006304.01.T01 | MTR_0256s0080 | 64.384 | 73 | 15 | 1 | 304 | 376 | 124 | 185 | 1.07e-19 | 90.5 |
| MsG0280006304.01.T01 | MTR_6g045925 | 47.917 | 96 | 30 | 3 | 89 | 166 | 4 | 97 | 2.49e-13 | 71.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006304.01.T01 | AT3G56150 | 60.369 | 434 | 131 | 6 | 1 | 405 | 1 | 422 | 1.19e-164 | 486 |
| MsG0280006304.01.T01 | AT3G56150 | 60.369 | 434 | 131 | 6 | 1 | 405 | 1 | 422 | 1.19e-164 | 486 |
| MsG0280006304.01.T01 | AT3G22860 | 47.381 | 420 | 164 | 12 | 3 | 405 | 5 | 384 | 1.53e-100 | 317 |
Find 106 sgRNAs with CRISPR-Local
Find 131 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGGTTGTTATGAGAATTT+AGG | 0.156316 | 2:+260543 | None:intergenic |
| CAAGAAGTTCAAAGAGATTC+TGG | 0.204715 | 2:-259622 | MsG0280006304.01.T01:CDS |
| GCTGGGACTCATCTTCTTCT+TGG | 0.219134 | 2:+259985 | None:intergenic |
| TGTAAAGATTAGGGATCTTT+TGG | 0.232109 | 2:+260065 | None:intergenic |
| AACCAATGGCTTCGAGATTC+TGG | 0.262577 | 2:-260517 | MsG0280006304.01.T01:exon |
| TGCTCAGGCTTCTGCTAATA+AGG | 0.263529 | 2:-260011 | MsG0280006304.01.T01:CDS |
| CTGGTTGTTATGAGAATTTA+GGG | 0.266616 | 2:+260544 | None:intergenic |
| GAATCATCAACCTTTATGTT+TGG | 0.286060 | 2:+259378 | None:intergenic |
| TTTGGTTTGTAGGTATCTAT+TGG | 0.296703 | 2:+260288 | None:intergenic |
| CAACAACGGGTTTGGTTTGT+AGG | 0.300152 | 2:+260278 | None:intergenic |
| TCCAGTTCCCTTCCTGCCCC+TGG | 0.302069 | 2:+259596 | None:intergenic |
| CCTTTATGAGCACGATAAAA+TGG | 0.316849 | 2:-259068 | MsG0280006304.01.T01:CDS |
| CAGATTTAACAACACGCTTT+TGG | 0.326666 | 2:+260230 | None:intergenic |
| GTTGAACTCATTACACAAAA+TGG | 0.327400 | 2:-259096 | MsG0280006304.01.T01:CDS |
| AATCATCAACCTTTATGTTT+GGG | 0.349179 | 2:+259379 | None:intergenic |
| TCTATTGGCTGTATTGTTGT+TGG | 0.355511 | 2:+260303 | None:intergenic |
| TAGCAAGAAAGACAGGCTTT+TGG | 0.359604 | 2:-259694 | MsG0280006304.01.T01:CDS |
| CAATGCTTTCAAGATATTCC+TGG | 0.380146 | 2:+259157 | None:intergenic |
| TCCGGCTTCCTGAGCTGATC+AGG | 0.393176 | 2:+259801 | None:intergenic |
| AGATTTCAAAGCTTCTTCTA+AGG | 0.393340 | 2:-259133 | MsG0280006304.01.T01:CDS |
| CTATTGGCTGTATTGTTGTT+GGG | 0.393873 | 2:+260304 | None:intergenic |
| GCCAGGGGCAGGAAGGGAAC+TGG | 0.399839 | 2:-259597 | MsG0280006304.01.T01:CDS |
| TGATGATGGCGATGCTCCTT+GGG | 0.400541 | 2:-259727 | MsG0280006304.01.T01:CDS |
| GCTCAGTTTGATGTTAATCC+AGG | 0.401276 | 2:-259483 | MsG0280006304.01.T01:CDS |
| TCGAAGCCATTGGTTTAGTC+TGG | 0.401988 | 2:+260525 | None:intergenic |
| AGAGCCTTGATGTAAAGATT+AGG | 0.402756 | 2:+260055 | None:intergenic |
| GTGATGATGGCGATGCTCCT+TGG | 0.404468 | 2:-259728 | MsG0280006304.01.T01:CDS |
| GTCCTGAGAGTGAGGGGAAA+AGG | 0.405233 | 2:-259877 | MsG0280006304.01.T01:intron |
| GCTTGAAGATTTCCTTGCTC+AGG | 0.408980 | 2:-260026 | MsG0280006304.01.T01:CDS |
| GAGCCTTGATGTAAAGATTA+GGG | 0.415850 | 2:+260056 | None:intergenic |
| ATGACGGACCCATTCATGTC+TGG | 0.416244 | 2:-259314 | MsG0280006304.01.T01:CDS |
| ACTGTCATCAACAACGGGTT+TGG | 0.429963 | 2:+260270 | None:intergenic |
| AATGAGTTCAACCCTCTTCA+AGG | 0.433459 | 2:+259107 | None:intergenic |
| CGTGCAGCACCCAAACATAA+AGG | 0.438376 | 2:-259388 | MsG0280006304.01.T01:CDS |
| TCAAAGAGATTCTGGAAGCC+AGG | 0.439840 | 2:-259614 | MsG0280006304.01.T01:CDS |
| GGCATATGCCCACTGAGGCC+TGG | 0.449256 | 2:+259465 | None:intergenic |
| ATGAGAATGAAACTAAGAAA+GGG | 0.464510 | 2:-259344 | MsG0280006304.01.T01:CDS |
| TCAGAATCCGACACGGGCTC+CGG | 0.466090 | 2:+259783 | None:intergenic |
| AGCTGCAGTTTCTTCTTCAT+CGG | 0.482972 | 2:-260600 | MsG0280006304.01.T01:five_prime_UTR |
| GGGAACTGGAAGGTTTGAAC+AGG | 0.485450 | 2:-259583 | MsG0280006304.01.T01:CDS |
| GATTCTGAAACTTCTGAGCT+TGG | 0.486351 | 2:-259768 | MsG0280006304.01.T01:CDS |
| TTGTTGGGTTCATCATCAAC+GGG | 0.486651 | 2:+260319 | None:intergenic |
| TAAGTTGCCCCAGACATGAA+TGG | 0.490965 | 2:+259305 | None:intergenic |
| GTGAATATGTTGAGAGACTC+AGG | 0.493281 | 2:-259215 | MsG0280006304.01.T01:CDS |
| GTTGTTGGGTTCATCATCAA+CGG | 0.494263 | 2:+260318 | None:intergenic |
| TCGTCACTGTCATCAACAAC+GGG | 0.495380 | 2:+260265 | None:intergenic |
| TGACGGACCCATTCATGTCT+GGG | 0.497542 | 2:-259313 | MsG0280006304.01.T01:CDS |
| CTTGGTAATGACAGGCCTCC+TGG | 0.498069 | 2:-259750 | MsG0280006304.01.T01:CDS |
| CAGGAATATCTTGAAAGCAT+TGG | 0.498525 | 2:-259156 | MsG0280006304.01.T01:CDS |
| CTGGAAGCCAGGGGCAGGAA+GGG | 0.505830 | 2:-259603 | MsG0280006304.01.T01:CDS |
| TCAACGGGTTCAGTATCGTT+AGG | 0.505992 | 2:+260334 | None:intergenic |
| ATGTTAATCCAGGCCTCAGT+GGG | 0.508312 | 2:-259473 | MsG0280006304.01.T01:CDS |
| AGATTCTGGAAGCCAGGGGC+AGG | 0.509743 | 2:-259608 | MsG0280006304.01.T01:CDS |
| AGAATCTGCAGCTTCTGAGC+AGG | 0.511073 | 2:+259522 | None:intergenic |
| AAGTTGCCCCAGACATGAAT+GGG | 0.512091 | 2:+259306 | None:intergenic |
| GATGAGAATGAAACTAAGAA+AGG | 0.515170 | 2:-259345 | MsG0280006304.01.T01:CDS |
| ACCTGATCAGCTCAGGAAGC+CGG | 0.516893 | 2:-259802 | MsG0280006304.01.T01:CDS |
| TCATTGAACCTGATCAGCTC+AGG | 0.519230 | 2:-259809 | MsG0280006304.01.T01:CDS |
| GATCCCTAATCTTTACATCA+AGG | 0.521376 | 2:-260059 | MsG0280006304.01.T01:CDS |
| CTCAACATATTCACGTGTGT+GGG | 0.525787 | 2:+259224 | None:intergenic |
| TGTTCTTGCACAGAATGTCC+AGG | 0.530188 | 2:-259175 | MsG0280006304.01.T01:CDS |
| AAAAGCTTAGCAAGAAAGAC+AGG | 0.531266 | 2:-259701 | MsG0280006304.01.T01:CDS |
| GGAAAAGGATGACGAGGATG+AGG | 0.531635 | 2:-259862 | MsG0280006304.01.T01:CDS |
| GTCCAGAATCTCGAAGCCAT+TGG | 0.533546 | 2:+260515 | None:intergenic |
| GACAGGCCTCCTGGTGATGA+TGG | 0.536973 | 2:-259741 | MsG0280006304.01.T01:CDS |
| ATGCTATCAAGATCAATGAC+TGG | 0.537887 | 2:-260156 | MsG0280006304.01.T01:CDS |
| CTTCTGAGCTTGGTAATGAC+AGG | 0.550457 | 2:-259758 | MsG0280006304.01.T01:CDS |
| CATAAAGGTTGATGATTCTG+TGG | 0.552363 | 2:-259373 | MsG0280006304.01.T01:CDS |
| CTTCTAAGGTAGCCTTGAAG+AGG | 0.561694 | 2:-259119 | MsG0280006304.01.T01:CDS |
| CAAAGAGATTCTGGAAGCCA+GGG | 0.563451 | 2:-259613 | MsG0280006304.01.T01:CDS |
| TCTGGAAGCCAGGGGCAGGA+AGG | 0.564829 | 2:-259604 | MsG0280006304.01.T01:CDS |
| GTGTCCCATGTGATCTCACT+AGG | 0.569429 | 2:+259651 | None:intergenic |
| GGAGCATCGCCATCATCACC+AGG | 0.573601 | 2:+259732 | None:intergenic |
| TCTCAACATATTCACGTGTG+TGG | 0.579942 | 2:+259223 | None:intergenic |
| AAAAGCCTAGTGAGATCACA+TGG | 0.585381 | 2:-259656 | MsG0280006304.01.T01:CDS |
| AGGTTCAATGATCTCATCAT+CGG | 0.587424 | 2:+259821 | None:intergenic |
| TGAGAATGAAACTAAGAAAG+GGG | 0.591503 | 2:-259343 | MsG0280006304.01.T01:CDS |
| GGTTCATCATCAATATCAGT+GGG | 0.592619 | 2:+260355 | None:intergenic |
| AGGTTCATCATCAATATCAG+TGG | 0.593036 | 2:+260354 | None:intergenic |
| TTCTAAGGTAGCCTTGAAGA+GGG | 0.593307 | 2:-259118 | MsG0280006304.01.T01:CDS |
| TGCTATCAAGATCAATGACT+GGG | 0.597590 | 2:-260155 | MsG0280006304.01.T01:CDS |
| GTGAGGGGAAAAGGATGACG+AGG | 0.602232 | 2:-259868 | MsG0280006304.01.T01:intron |
| GAACTCATTACACAAAATGG+AGG | 0.604238 | 2:-259093 | MsG0280006304.01.T01:CDS |
| CAGGAAGCCGGAGCCCGTGT+CGG | 0.608070 | 2:-259790 | MsG0280006304.01.T01:CDS |
| GACAGTGACGACGACGATGA+TGG | 0.616344 | 2:-260253 | MsG0280006304.01.T01:CDS |
| TAACAACCAGACTAAACCAA+TGG | 0.622757 | 2:-260531 | MsG0280006304.01.T01:exon |
| TATTAGCAGAAGCCTGAGCA+AGG | 0.625753 | 2:+260014 | None:intergenic |
| GTCGTCACTGTCATCAACAA+CGG | 0.633735 | 2:+260264 | None:intergenic |
| AACTCATTACACAAAATGGA+GGG | 0.636192 | 2:-259092 | MsG0280006304.01.T01:CDS |
| AAGAAAGGGGATGACTATGA+CGG | 0.642172 | 2:-259330 | MsG0280006304.01.T01:CDS |
| TCAACATATTCACGTGTGTG+GGG | 0.645878 | 2:+259225 | None:intergenic |
| GGCTTCGAGATTCTGGACAC+AGG | 0.648433 | 2:-260510 | MsG0280006304.01.T01:intron |
| GAAGTTTCAGAATCCGACAC+GGG | 0.650738 | 2:+259777 | None:intergenic |
| AAAATGCGTGCAGAATATGC+TGG | 0.655482 | 2:-259427 | MsG0280006304.01.T01:CDS |
| GGGGCAGGAAGGGAACTGGA+AGG | 0.655940 | 2:-259593 | MsG0280006304.01.T01:CDS |
| GATGTTAATCCAGGCCTCAG+TGG | 0.669943 | 2:-259474 | MsG0280006304.01.T01:CDS |
| TGTGCAAGAACAACAAACTG+AGG | 0.676475 | 2:+259186 | None:intergenic |
| GGCATATGCCCATAAATGTG+TGG | 0.678845 | 2:-259452 | MsG0280006304.01.T01:CDS |
| GACGGACCCATTCATGTCTG+GGG | 0.680909 | 2:-259312 | MsG0280006304.01.T01:CDS |
| TGAATATGTTGAGAGACTCA+GGG | 0.682868 | 2:-259214 | MsG0280006304.01.T01:CDS |
| AGAAGTTTCAGAATCCGACA+CGG | 0.685846 | 2:+259776 | None:intergenic |
| ATTCTTGATCTGATCAACAG+TGG | 0.701566 | 2:+260177 | None:intergenic |
| AAAGAGATTCTGGAAGCCAG+GGG | 0.701656 | 2:-259612 | MsG0280006304.01.T01:CDS |
| GCATCGCCATCATCACCAGG+AGG | 0.707685 | 2:+259735 | None:intergenic |
| AAAGCCTAGTGAGATCACAT+GGG | 0.747188 | 2:-259655 | MsG0280006304.01.T01:CDS |
| TTATGGGCATATGCCCACTG+AGG | 0.770132 | 2:+259460 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATCATCAACCTTTATGTTT+GGG | + | Chr2:260297-260316 | None:intergenic | 25.0% |
| ! | ATGAGAATGAAACTAAGAAA+GGG | - | Chr2:260329-260348 | MsG0280006304.01.T01:CDS | 25.0% |
| !!! | CATTTTTTCCACACATTTAT+GGG | + | Chr2:260232-260251 | None:intergenic | 25.0% |
| AACTCATTACACAAAATGGA+GGG | - | Chr2:260581-260600 | MsG0280006304.01.T01:five_prime_UTR | 30.0% | |
| AGATTTCAAAGCTTCTTCTA+AGG | - | Chr2:260540-260559 | MsG0280006304.01.T01:five_prime_UTR | 30.0% | |
| AGGACTTGATTATCAAATGT+AGG | - | Chr2:259769-259788 | MsG0280006304.01.T01:CDS | 30.0% | |
| CTGGTTGTTATGAGAATTTA+GGG | + | Chr2:259132-259151 | None:intergenic | 30.0% | |
| GAATCATCAACCTTTATGTT+TGG | + | Chr2:260298-260317 | None:intergenic | 30.0% | |
| GATGAGAATGAAACTAAGAA+AGG | - | Chr2:260328-260347 | MsG0280006304.01.T01:CDS | 30.0% | |
| GGACTTGATTATCAAATGTA+GGG | - | Chr2:259770-259789 | MsG0280006304.01.T01:CDS | 30.0% | |
| GTTGAACTCATTACACAAAA+TGG | - | Chr2:260577-260596 | MsG0280006304.01.T01:five_prime_UTR | 30.0% | |
| TCTGGTTGTTATGAGAATTT+AGG | + | Chr2:259133-259152 | None:intergenic | 30.0% | |
| TGAGAATGAAACTAAGAAAG+GGG | - | Chr2:260330-260349 | MsG0280006304.01.T01:CDS | 30.0% | |
| ! | GTAAAGATTAGGGATCTTTT+GGG | + | Chr2:259610-259629 | None:intergenic | 30.0% |
| ! | TGTAAAGATTAGGGATCTTT+TGG | + | Chr2:259611-259630 | None:intergenic | 30.0% |
| ! | TTTAGCAACTTTTGTTAGGA+AGG | + | Chr2:260128-260147 | None:intergenic | 30.0% |
| !! | TTTGGTTTGTAGGTATCTAT+TGG | + | Chr2:259388-259407 | None:intergenic | 30.0% |
| !!! | GCATTTTTTCCACACATTTA+TGG | + | Chr2:260233-260252 | None:intergenic | 30.0% |
| !!! | GTGTTTTAGCAACTTTTGTT+AGG | + | Chr2:260132-260151 | None:intergenic | 30.0% |
| !!! | TTTTTGCTTCATTGAGTTGA+AGG | + | Chr2:259724-259743 | None:intergenic | 30.0% |
| AAAAGCTTAGCAAGAAAGAC+AGG | - | Chr2:259972-259991 | MsG0280006304.01.T01:intron | 35.0% | |
| AGAGCCTTGATGTAAAGATT+AGG | + | Chr2:259621-259640 | None:intergenic | 35.0% | |
| AGGTTCAATGATCTCATCAT+CGG | + | Chr2:259855-259874 | None:intergenic | 35.0% | |
| AGGTTCATCATCAATATCAG+TGG | + | Chr2:259322-259341 | None:intergenic | 35.0% | |
| ATGCTATCAAGATCAATGAC+TGG | - | Chr2:259517-259536 | MsG0280006304.01.T01:CDS | 35.0% | |
| ATTCTTGATCTGATCAACAG+TGG | + | Chr2:259499-259518 | None:intergenic | 35.0% | |
| CAAGAAGTTCAAAGAGATTC+TGG | - | Chr2:260051-260070 | MsG0280006304.01.T01:CDS | 35.0% | |
| CAATGCTTTCAAGATATTCC+TGG | + | Chr2:260519-260538 | None:intergenic | 35.0% | |
| CCTTTATGAGCACGATAAAA+TGG | - | Chr2:260605-260624 | MsG0280006304.01.T01:five_prime_UTR | 35.0% | |
| GAACTCATTACACAAAATGG+AGG | - | Chr2:260580-260599 | MsG0280006304.01.T01:five_prime_UTR | 35.0% | |
| GAAGAACAATAAGCAATACG+AGG | - | Chr2:259749-259768 | MsG0280006304.01.T01:CDS | 35.0% | |
| GAGCCTTGATGTAAAGATTA+GGG | + | Chr2:259620-259639 | None:intergenic | 35.0% | |
| GATCCCTAATCTTTACATCA+AGG | - | Chr2:259614-259633 | MsG0280006304.01.T01:CDS | 35.0% | |
| GGTTCATCATCAATATCAGT+GGG | + | Chr2:259321-259340 | None:intergenic | 35.0% | |
| TAACAACCAGACTAAACCAA+TGG | - | Chr2:259142-259161 | MsG0280006304.01.T01:CDS | 35.0% | |
| TGAATAAACACACAGAGAGA+CGG | + | Chr2:259240-259259 | None:intergenic | 35.0% | |
| TGAATATGTTGAGAGACTCA+GGG | - | Chr2:260459-260478 | MsG0280006304.01.T01:intron | 35.0% | |
| TGCTATCAAGATCAATGACT+GGG | - | Chr2:259518-259537 | MsG0280006304.01.T01:CDS | 35.0% | |
| ! | CAGATTTAACAACACGCTTT+TGG | + | Chr2:259446-259465 | None:intergenic | 35.0% |
| ! | CAGGAATATCTTGAAAGCAT+TGG | - | Chr2:260517-260536 | MsG0280006304.01.T01:exon | 35.0% |
| ! | CCATTTTATCGTGCTCATAA+AGG | + | Chr2:260608-260627 | None:intergenic | 35.0% |
| ! | CTATTGGCTGTATTGTTGTT+GGG | + | Chr2:259372-259391 | None:intergenic | 35.0% |
| ! | TCTATTGGCTGTATTGTTGT+TGG | + | Chr2:259373-259392 | None:intergenic | 35.0% |
| !! | AGATAAGCGTTTTGATGAGA+TGG | - | Chr2:259470-259489 | MsG0280006304.01.T01:CDS | 35.0% |
| !! | CATAAAGGTTGATGATTCTG+TGG | - | Chr2:260300-260319 | MsG0280006304.01.T01:CDS | 35.0% |
| AAAAGCCTAGTGAGATCACA+TGG | - | Chr2:260017-260036 | MsG0280006304.01.T01:CDS | 40.0% | |
| AAAATGCGTGCAGAATATGC+TGG | - | Chr2:260246-260265 | MsG0280006304.01.T01:CDS | 40.0% | |
| AAAGCCTAGTGAGATCACAT+GGG | - | Chr2:260018-260037 | MsG0280006304.01.T01:CDS | 40.0% | |
| AAGAAAGGGGATGACTATGA+CGG | - | Chr2:260343-260362 | MsG0280006304.01.T01:CDS | 40.0% | |
| AATGAGTTCAACCCTCTTCA+AGG | + | Chr2:260569-260588 | None:intergenic | 40.0% | |
| AGAAGTTTCAGAATCCGACA+CGG | + | Chr2:259900-259919 | None:intergenic | 40.0% | |
| AGCTGCAGTTTCTTCTTCAT+CGG | - | Chr2:259073-259092 | MsG0280006304.01.T01:CDS | 40.0% | |
| CTCAACATATTCACGTGTGT+GGG | + | Chr2:260452-260471 | None:intergenic | 40.0% | |
| CTGCAAGCTCTTGAAAAACT+CGG | + | Chr2:260419-260438 | None:intergenic | 40.0% | |
| GCTCAGTTTGATGTTAATCC+AGG | - | Chr2:260190-260209 | MsG0280006304.01.T01:CDS | 40.0% | |
| GTGAATATGTTGAGAGACTC+AGG | - | Chr2:260458-260477 | MsG0280006304.01.T01:intron | 40.0% | |
| GTTGTTGGGTTCATCATCAA+CGG | + | Chr2:259358-259377 | None:intergenic | 40.0% | |
| TCAACATATTCACGTGTGTG+GGG | + | Chr2:260451-260470 | None:intergenic | 40.0% | |
| TCTCAACATATTCACGTGTG+TGG | + | Chr2:260453-260472 | None:intergenic | 40.0% | |
| TGTGCAAGAACAACAAACTG+AGG | + | Chr2:260490-260509 | None:intergenic | 40.0% | |
| TTGTTGGGTTCATCATCAAC+GGG | + | Chr2:259357-259376 | None:intergenic | 40.0% | |
| ! | TAGCAAGAAAGACAGGCTTT+TGG | - | Chr2:259979-259998 | MsG0280006304.01.T01:intron | 40.0% |
| ! | TTGCTAAGCTTTTGATCCCA+AGG | + | Chr2:259965-259984 | None:intergenic | 40.0% |
| !! | CTTCATTGAGTTGAAGGCTT+TGG | + | Chr2:259718-259737 | None:intergenic | 40.0% |
| !! | GATTCTGAAACTTCTGAGCT+TGG | - | Chr2:259905-259924 | MsG0280006304.01.T01:intron | 40.0% |
| !! | TTCTAAGGTAGCCTTGAAGA+GGG | - | Chr2:260555-260574 | MsG0280006304.01.T01:five_prime_UTR | 40.0% |
| AACCAATGGCTTCGAGATTC+TGG | - | Chr2:259156-259175 | MsG0280006304.01.T01:CDS | 45.0% | |
| AAGTTGCCCCAGACATGAAT+GGG | + | Chr2:260370-260389 | None:intergenic | 45.0% | |
| ATGTTAATCCAGGCCTCAGT+GGG | - | Chr2:260200-260219 | MsG0280006304.01.T01:CDS | 45.0% | |
| GAAGTTTCAGAATCCGACAC+GGG | + | Chr2:259899-259918 | None:intergenic | 45.0% | |
| GCTTGAAGATTTCCTTGCTC+AGG | - | Chr2:259647-259666 | MsG0280006304.01.T01:CDS | 45.0% | |
| GGCATATGCCCATAAATGTG+TGG | - | Chr2:260221-260240 | MsG0280006304.01.T01:CDS | 45.0% | |
| GTCGTCACTGTCATCAACAA+CGG | + | Chr2:259412-259431 | None:intergenic | 45.0% | |
| TAAGTTGCCCCAGACATGAA+TGG | + | Chr2:260371-260390 | None:intergenic | 45.0% | |
| TATTAGCAGAAGCCTGAGCA+AGG | + | Chr2:259662-259681 | None:intergenic | 45.0% | |
| TCATTGAACCTGATCAGCTC+AGG | - | Chr2:259864-259883 | MsG0280006304.01.T01:CDS | 45.0% | |
| TCGTCACTGTCATCAACAAC+GGG | + | Chr2:259411-259430 | None:intergenic | 45.0% | |
| TGCTCAGGCTTCTGCTAATA+AGG | - | Chr2:259662-259681 | MsG0280006304.01.T01:CDS | 45.0% | |
| TGTTCTTGCACAGAATGTCC+AGG | - | Chr2:260498-260517 | MsG0280006304.01.T01:intron | 45.0% | |
| ! | AAAGAGATTCTGGAAGCCAG+GGG | - | Chr2:260061-260080 | MsG0280006304.01.T01:CDS | 45.0% |
| ! | ACTGTCATCAACAACGGGTT+TGG | + | Chr2:259406-259425 | None:intergenic | 45.0% |
| ! | CAAAGAGATTCTGGAAGCCA+GGG | - | Chr2:260060-260079 | MsG0280006304.01.T01:CDS | 45.0% |
| ! | GCTGCAGATTCTTTTCAGTG+TGG | - | Chr2:260162-260181 | MsG0280006304.01.T01:CDS | 45.0% |
| ! | TCAAAGAGATTCTGGAAGCC+AGG | - | Chr2:260059-260078 | MsG0280006304.01.T01:CDS | 45.0% |
| ! | TCAACGGGTTCAGTATCGTT+AGG | + | Chr2:259342-259361 | None:intergenic | 45.0% |
| ! | TTGAAGGCTTTGGCATTGCT+GGG | + | Chr2:259708-259727 | None:intergenic | 45.0% |
| !! | CAACAACGGGTTTGGTTTGT+AGG | + | Chr2:259398-259417 | None:intergenic | 45.0% |
| !! | CTTCTAAGGTAGCCTTGAAG+AGG | - | Chr2:260554-260573 | MsG0280006304.01.T01:five_prime_UTR | 45.0% |
| !! | CTTCTGAGCTTGGTAATGAC+AGG | - | Chr2:259915-259934 | MsG0280006304.01.T01:intron | 45.0% |
| !! | TCGAAGCCATTGGTTTAGTC+TGG | + | Chr2:259151-259170 | None:intergenic | 45.0% |
| !!! | GGTTTTGTTTTGCGCGATGA+AGG | + | Chr2:259111-259130 | None:intergenic | 45.0% |
| !! | TATTATTATTATTATTCTTA+GGG | - | Chr2:259270-259289 | MsG0280006304.01.T01:CDS | 5.0% |
| !! | TTATTATTATTATTATTCTT+AGG | - | Chr2:259269-259288 | MsG0280006304.01.T01:CDS | 5.0% |
| AGAATCTGCAGCTTCTGAGC+AGG | + | Chr2:260154-260173 | None:intergenic | 50.0% | |
| CGTGCAGCACCCAAACATAA+AGG | - | Chr2:260285-260304 | MsG0280006304.01.T01:CDS | 50.0% | |
| GATGTTAATCCAGGCCTCAG+TGG | - | Chr2:260199-260218 | MsG0280006304.01.T01:CDS | 50.0% | |
| GCTGGGACTCATCTTCTTCT+TGG | + | Chr2:259691-259710 | None:intergenic | 50.0% | |
| GGAAAAGGATGACGAGGATG+AGG | - | Chr2:259811-259830 | MsG0280006304.01.T01:CDS | 50.0% | |
| GGGAACTGGAAGGTTTGAAC+AGG | - | Chr2:260090-260109 | MsG0280006304.01.T01:CDS | 50.0% | |
| GTCCAGAATCTCGAAGCCAT+TGG | + | Chr2:259161-259180 | None:intergenic | 50.0% | |
| GTGTCCCATGTGATCTCACT+AGG | + | Chr2:260025-260044 | None:intergenic | 50.0% | |
| TTATGGGCATATGCCCACTG+AGG | + | Chr2:260216-260235 | None:intergenic | 50.0% | |
| ! | ATCCTTTTCCCCTCACTCTC+AGG | + | Chr2:259801-259820 | None:intergenic | 50.0% |
| ! | GTTGAAGGCTTTGGCATTGC+TGG | + | Chr2:259709-259728 | None:intergenic | 50.0% |
| ! | TGACGGACCCATTCATGTCT+GGG | - | Chr2:260360-260379 | MsG0280006304.01.T01:CDS | 50.0% |
| !! | ATGACGGACCCATTCATGTC+TGG | - | Chr2:260359-260378 | MsG0280006304.01.T01:CDS | 50.0% |
| !! | TGATGATGGCGATGCTCCTT+GGG | - | Chr2:259946-259965 | MsG0280006304.01.T01:intron | 50.0% |
| ACCTGATCAGCTCAGGAAGC+CGG | - | Chr2:259871-259890 | MsG0280006304.01.T01:intron | 55.0% | |
| AGGGAGAGTCCTGAGAGTGA+GGG | - | Chr2:259789-259808 | MsG0280006304.01.T01:CDS | 55.0% | |
| GACAGTGACGACGACGATGA+TGG | - | Chr2:259420-259439 | MsG0280006304.01.T01:CDS | 55.0% | |
| GACGGACCCATTCATGTCTG+GGG | - | Chr2:260361-260380 | MsG0280006304.01.T01:CDS | 55.0% | |
| GGCTTCGAGATTCTGGACAC+AGG | - | Chr2:259163-259182 | MsG0280006304.01.T01:CDS | 55.0% | |
| GTCCTGAGAGTGAGGGGAAA+AGG | - | Chr2:259796-259815 | MsG0280006304.01.T01:CDS | 55.0% | |
| GTGAGGGGAAAAGGATGACG+AGG | - | Chr2:259805-259824 | MsG0280006304.01.T01:CDS | 55.0% | |
| TAGGGAGAGTCCTGAGAGTG+AGG | - | Chr2:259788-259807 | MsG0280006304.01.T01:CDS | 55.0% | |
| !! | CTTGGTAATGACAGGCCTCC+TGG | - | Chr2:259923-259942 | MsG0280006304.01.T01:intron | 55.0% |
| !! | GTGATGATGGCGATGCTCCT+TGG | - | Chr2:259945-259964 | MsG0280006304.01.T01:intron | 55.0% |
| GCATCGCCATCATCACCAGG+AGG | + | Chr2:259941-259960 | None:intergenic | 60.0% | |
| GGGAGAGTCCTGAGAGTGAG+GGG | - | Chr2:259790-259809 | MsG0280006304.01.T01:CDS | 60.0% | |
| TCAGAATCCGACACGGGCTC+CGG | + | Chr2:259893-259912 | None:intergenic | 60.0% | |
| TCCGGCTTCCTGAGCTGATC+AGG | + | Chr2:259875-259894 | None:intergenic | 60.0% | |
| ! | AGATTCTGGAAGCCAGGGGC+AGG | - | Chr2:260065-260084 | MsG0280006304.01.T01:CDS | 60.0% |
| ! | GACAGGCCTCCTGGTGATGA+TGG | - | Chr2:259932-259951 | MsG0280006304.01.T01:intron | 60.0% |
| !! | GGAGCATCGCCATCATCACC+AGG | + | Chr2:259944-259963 | None:intergenic | 60.0% |
| CTGGAAGCCAGGGGCAGGAA+GGG | - | Chr2:260070-260089 | MsG0280006304.01.T01:CDS | 65.0% | |
| GGCATATGCCCACTGAGGCC+TGG | + | Chr2:260211-260230 | None:intergenic | 65.0% | |
| GGGGCAGGAAGGGAACTGGA+AGG | - | Chr2:260080-260099 | MsG0280006304.01.T01:CDS | 65.0% | |
| TCCAGTTCCCTTCCTGCCCC+TGG | + | Chr2:260080-260099 | None:intergenic | 65.0% | |
| TCTGGAAGCCAGGGGCAGGA+AGG | - | Chr2:260069-260088 | MsG0280006304.01.T01:CDS | 65.0% | |
| CAGGAAGCCGGAGCCCGTGT+CGG | - | Chr2:259883-259902 | MsG0280006304.01.T01:intron | 70.0% | |
| GCCAGGGGCAGGAAGGGAAC+TGG | - | Chr2:260076-260095 | MsG0280006304.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 259062 | 260633 | 259062 | ID=MsG0280006304.01;Name=MsG0280006304.01 |
| Chr2 | mRNA | 259062 | 260633 | 259062 | ID=MsG0280006304.01.T01;Parent=MsG0280006304.01;Name=MsG0280006304.01.T01;_AED=0.47;_eAED=0.47;_QI=99|0.5|0.33|1|1|1|3|0|419 |
| Chr2 | exon | 259062 | 259885 | 259062 | ID=MsG0280006304.01.T01:exon:579;Parent=MsG0280006304.01.T01 |
| Chr2 | exon | 259993 | 260404 | 259993 | ID=MsG0280006304.01.T01:exon:578;Parent=MsG0280006304.01.T01 |
| Chr2 | exon | 260511 | 260633 | 260511 | ID=MsG0280006304.01.T01:exon:577;Parent=MsG0280006304.01.T01 |
| Chr2 | five_prime_UTR | 260535 | 260633 | 260535 | ID=MsG0280006304.01.T01:five_prime_utr;Parent=MsG0280006304.01.T01 |
| Chr2 | CDS | 260511 | 260534 | 260511 | ID=MsG0280006304.01.T01:cds;Parent=MsG0280006304.01.T01 |
| Chr2 | CDS | 259993 | 260404 | 259993 | ID=MsG0280006304.01.T01:cds;Parent=MsG0280006304.01.T01 |
| Chr2 | CDS | 259062 | 259885 | 259062 | ID=MsG0280006304.01.T01:cds;Parent=MsG0280006304.01.T01 |
| Gene Sequence |
| Protein sequence |
